CN111286519B - 富含dha藻油的生产、提取以及纯化工艺 - Google Patents
富含dha藻油的生产、提取以及纯化工艺 Download PDFInfo
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- CN111286519B CN111286519B CN202010281973.2A CN202010281973A CN111286519B CN 111286519 B CN111286519 B CN 111286519B CN 202010281973 A CN202010281973 A CN 202010281973A CN 111286519 B CN111286519 B CN 111286519B
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- chloroform
- seed
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/106—Production of fats or fatty oils from raw materials by extracting using ultra-sounds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
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- Life Sciences & Earth Sciences (AREA)
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Abstract
Description
组别 | 实施例1 | 对照组1 | 对照组2 | 对照组3 |
C22:6 | 57.69 | 53.07 | 51.24 | 47.76 |
C22:5 | 9.13 | 10.13 | 11.46 | 13.31 |
C16:0 | 18.22 | 21.34 | 20.69 | 23.57 |
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202010281973.2A CN111286519B (zh) | 2018-09-25 | 2018-09-25 | 富含dha藻油的生产、提取以及纯化工艺 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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CN202010281973.2A CN111286519B (zh) | 2018-09-25 | 2018-09-25 | 富含dha藻油的生产、提取以及纯化工艺 |
CN201811111845.2A CN109055448B (zh) | 2018-09-25 | 2018-09-25 | 富含dha藻油的生产、提取以及纯化工艺 |
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CN201811111845.2A Division CN109055448B (zh) | 2018-09-25 | 2018-09-25 | 富含dha藻油的生产、提取以及纯化工艺 |
Publications (2)
Publication Number | Publication Date |
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CN111286519A CN111286519A (zh) | 2020-06-16 |
CN111286519B true CN111286519B (zh) | 2021-10-26 |
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Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1359224A1 (en) * | 2002-05-01 | 2003-11-05 | Ato B.V. | A process for production of polyunsaturated fatty acids by marine microorganisms |
CN1986822A (zh) * | 2006-12-15 | 2007-06-27 | 湖北友芝友生物科技有限公司 | 用寇氏隐甲藻发酵生产二十二碳六烯酸油脂的方法 |
CN101133144A (zh) * | 2004-12-23 | 2008-02-27 | 努特诺瓦营养产品及食品成分有限公司 | 从微生物和植物的混合物中制备粗制油的方法,所述方法制备的油,由此制备的油和任选另外精炼的油的特定用途 |
US7989195B2 (en) * | 2008-02-20 | 2011-08-02 | Washington State University Research Foundation | Heterotrophic algal high cell density production method and system |
WO2011097276A8 (en) * | 2010-02-02 | 2011-10-20 | Martek Biosciences Corporation | Methods and compositions for treating arthritis with docosahexaenoic acid |
US8183227B1 (en) * | 2011-07-07 | 2012-05-22 | Chemo S. A. France | Compositions, kits and methods for nutrition supplementation |
CN103966273A (zh) * | 2014-04-29 | 2014-08-06 | 内蒙古金达威药业有限公司 | 一种双鞭甲藻发酵生产dha的方法 |
CN105670771A (zh) * | 2016-02-26 | 2016-06-15 | 深圳市荣格保健品有限公司 | 一种藻油dha提取制备方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2659603C (en) * | 2006-08-01 | 2017-03-07 | Ocean Nutrition Canada Limited | Oil producing microbes and methods of modification thereof |
CN101168501B (zh) * | 2007-07-11 | 2010-04-07 | 南京工业大学 | 一种从隐甲藻中提取并精制富含dha脂肪酸的工艺 |
CN101538592B (zh) * | 2009-04-28 | 2012-06-27 | 湖北福星生物科技有限公司 | 用寇氏隐甲藻工业化发酵生产二十二碳六烯酸的方法 |
CN104726508B (zh) * | 2015-02-03 | 2018-04-06 | 昆明藻能生物科技有限公司 | 多种化学促进剂提高微生物异养发酵生产二十二碳六烯酸的方法 |
-
2018
- 2018-09-25 CN CN202010281973.2A patent/CN111286519B/zh active Active
- 2018-09-25 CN CN201811111845.2A patent/CN109055448B/zh active Active
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1359224A1 (en) * | 2002-05-01 | 2003-11-05 | Ato B.V. | A process for production of polyunsaturated fatty acids by marine microorganisms |
CN101133144A (zh) * | 2004-12-23 | 2008-02-27 | 努特诺瓦营养产品及食品成分有限公司 | 从微生物和植物的混合物中制备粗制油的方法,所述方法制备的油,由此制备的油和任选另外精炼的油的特定用途 |
CN1986822A (zh) * | 2006-12-15 | 2007-06-27 | 湖北友芝友生物科技有限公司 | 用寇氏隐甲藻发酵生产二十二碳六烯酸油脂的方法 |
US7989195B2 (en) * | 2008-02-20 | 2011-08-02 | Washington State University Research Foundation | Heterotrophic algal high cell density production method and system |
WO2011097276A8 (en) * | 2010-02-02 | 2011-10-20 | Martek Biosciences Corporation | Methods and compositions for treating arthritis with docosahexaenoic acid |
US8183227B1 (en) * | 2011-07-07 | 2012-05-22 | Chemo S. A. France | Compositions, kits and methods for nutrition supplementation |
CN103966273A (zh) * | 2014-04-29 | 2014-08-06 | 内蒙古金达威药业有限公司 | 一种双鞭甲藻发酵生产dha的方法 |
CN105670771A (zh) * | 2016-02-26 | 2016-06-15 | 深圳市荣格保健品有限公司 | 一种藻油dha提取制备方法 |
Non-Patent Citations (2)
Title |
---|
Crypthecodinium cohnii with emphasis on DHA production: a review;Ana Mendes等;《Journal of Applied Phycology》;20080817;第21卷;第199-214页 * |
高产二十二碳六烯酸隐甲藻的选育及其发酵条件研究;孙中贯等;《食品科学》;20130531;第34卷(第05期);第202-206页 * |
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