CN111280092B - Artificial breeding method for acrossocheilus yunnanensis - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/445—Non condensed piperidines, e.g. piperocaine
- A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
- A61K31/454—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. pimozide, domperidone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/47—Quinolines; Isoquinolines
- A61K31/475—Quinolines; Isoquinolines having an indole ring, e.g. yohimbine, reserpine, strychnine, vinblastine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
- A61K38/09—Luteinising hormone-releasing hormone [LHRH], i.e. Gonadotropin-releasing hormone [GnRH]; Related peptides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention belongs to the technical field of cultivation, and particularly relates to an artificial breeding method of acrossocheilus yunnanensis, which comprises the following steps: the method disclosed by the invention has the advantages that the efficient breeding of the Yunnan Guangxi bare-lipped fishes is promoted by utilizing a proper environment and providing sufficient protein bait and hormone stimulation through the complementary of the three steps according to the nutritional requirements and the life habits of the Yunnan Guangxi bare-lipped fishes.
Description
Technical Field
The invention relates to the technical field of cultivation, in particular to an artificial breeding method of acrossocheilus yunnanensis.
Background
The Yunan Guangyi Dian fish is mainly distributed in Jinshajiang, Nanpan river and auxiliary lakes in Yunnan, and the resources are sharply reduced after 60 years of 20 th century until almost no longer in 80 years. The yunnan bare lipsticks are rich in nutrition, tender in meat quality and delicious in taste, and in order to protect and develop the yunnan bare lipsticks resource, the artificial propagation of the yunnan bare lipsticks is realized in 2014 through practices and researches of related technical personnel, and the artificial breeding of the yunnan bare lipsticks is performed in the southwest region and other places in recent years.
The Yunnan bare lipped is suitable for water temperature of 5-28 ℃, the most suitable water temperature of 14-24 ℃, is fond of living in a clear water environment, mainly lives in the upper middle layer of a water body, has strong reverse water jumping capability, is edible in the nature of food preference for other foods, can also eat compound feed, is mainly eaten by rotifers and fleas in a seedling stage, and can eat filamentous bathing, rotten organic scraps and the like more than 4 cm; the Yunnan Guangxi Kouchi fish is tender in quality, high in DHA and EPA content and popular with consumers, wild Yunnan Guangxi Kouchi fish resources are reduced sharply due to human activities such as habitat destruction and excessive fishing, the market price is high, the full-artificial propagation of the Yunnan Guangxi Kouchi fish is carried out, the large-scale production of seedlings is realized, the wild Yunnan Guangxi Kouchi fish resources are protected, the artificial propagation in natural water areas is met, and the urgent need of market supply is ensured; at present, the problems in the propagation of the Yunan Ophioglossus mainly comprise: the breeding parents are mostly caught by wild plants, the quantity cannot be guaranteed, the spawning is not concentrated, and the large-scale production of the seedlings is difficult to realize.
In the prior art, the research on the breeding and growth of the yunnan liptophagus is more, for example, a full-artificial breeding method of the yunnan liptophagus with the patent number of CN201611167311.2 achieves a higher breeding effect through juvenile fish domestication, parent selection and hormone use, but the research does not consider the whole growth environment of the fish, only focuses on the selection of fish bodies and the hormone use, and cannot maximally show the breeding performance of the yunnan liptophagus; for another example, a high-efficiency artificial propagation method for acrossocheilus yunnanensis with patent number CN201610109556.3 mainly achieves the purpose of high propagation by using a breeding device, but in documents, deep excavation is not performed on the ecological habit of the acrossocheilus yunnanensis, and the propagation performance of the acrossocheilus yunnanensis cannot be exerted to the maximum extent; therefore, the comprehensive consideration of nutrition and growth environment is lacked in the prior art, so that the breeding effect of the Yunan Ophioglossus can be improved to the maximum extent, and the large-scale breeding of the Yunan Ophioglossus is promoted.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention provides an artificial breeding method of acrossocheilus yunnanensis, which comprises the following steps:
(1) improvement of spawning pool
Injecting water into the spawning inducing pool until the pool is full, adding ozone into the water, covering the surface of the pool with a plastic film, standing for 50-60min to ensure that the ozone can fully kill pathogenic microorganisms in the pool, then emptying the water in the pool, and washing for 2-4 times with clear water; laying 3-5cm thick gravel at the bottom of the spawning inducing pool, so that the water quality in the water can be kept clean for a long time, and adding water to ensure that the water depth in the pool is 70-80 cm; the manufacturing method of the sand gravel comprises the following steps: mixing and crushing river sand and celestite into particles, flushing the particles for 3 to 5min by clear water with the flow rate of 1.5 to 2m/s, draining the particles, heating the particles to the temperature of 150 ℃ and 200 ℃ under the standard atmospheric pressure of 0.1 to 0.3, performing microwave treatment, naturally cooling the particles to the normal temperature, flushing the particles for 10 to 20min by the clear water, and drying the particles;
(2) stocking parent fish
Putting the sexually mature parent fish into an induced spawning pond, feeding biological extract feed for 1-2 weeks; the preparation method of the biological extraction feed comprises the following steps: silkworm chrysalis, locust, bamboo worms and red earthworms are mixed and pulped according to the mass ratio of 1:1:1:5, filtered by 200-mesh gauze of 300 meshes, and the obtained filtrate is dried under reduced pressure at the temperature of 20-25 ℃ until the water content is 30-35% for later use; drying the obtained filter residue at 30-35 deg.C under reduced pressure until the water content is 5-8%, and micronizing the filter residue for use; mixing the dried filtrate and the superfine crushed substances of the filter residue, and granulating to obtain the biological extraction feed;
(3) spawning induction of parent fish
Controlling the water temperature at 20-23 ℃ and the dissolved oxygen to be more than 9mg/L, injecting an oxytocic at the abdominal part of the parent fish without scales, wherein the first injection is 30 percent of the oxytocic, the second injection is 30 percent of the oxytocic, and the third injection is 40 percent of the oxytocic, and the injection time interval is 5-8 hours.
Furthermore, the concentration of the ozone in the aqueous solution is 0.5-0.8mg/L, so that the sterilizing effect of the ozone is ensured.
Furthermore, the mass ratio of the river sand to the celestite is 10: 1.
Further, the microwave treatment conditions are as follows: the power is 1200W-1500W, and the time is 10-15 min.
Furthermore, the crushing granularity of the river sand and celestite is 0.8-1.3cm, and the uniformity and the adsorption surface of the gravel are improved.
Furthermore, the granularity of the biological extraction feed is 40-60 meshes, so that the biological extraction feed is beneficial to digestion and absorption of the Yunan Ophioglossus.
Further, the dose of the oxytocic is as follows: luteinizing release hormone analogue 6ug/kg + altanone 4mg/kg + reserpine 1 mg/kg.
Advantageous effects
According to the invention, through the improvement of the induced spawning pond, the pond water is cleaner, the influence of pathogenic microorganisms on the Yunan lipped fishes is reduced, gravel is used at the bottom of the induced spawning pond, after microwave treatment, the roughness of the surface of the gravel is further improved, the adsorption efficiency is remarkably improved, after microwave treatment, the gravel is washed by clear water, and the treated gravel is slowly released in the pond water to form ions of iron, phosphorus and the like with low concentration in an aqueous solution to supplement the fishes; after the parent fish is put in the breeding, self-made high-protein feed is used for feeding, and silkworm pupas, cicadas, bamboo worms and red earthworms are used for preparing biological feed with balanced protein, vitamin and protein contents, so that the physiological function of the parent fish is effectively improved, the physiological state is improved, and the next induced spawning is facilitated; and finally, luteinizing hormone-releasing hormone analogue, DIWANONE and reserpine are used comprehensively, so that the egg laying rate and fertilization rate of the Yunan lipped fishes are improved.
The method provided by the invention fully meets the nutritional requirements and ecological habits of the Yunnan bare lipsticks, and utilizes a proper environment to provide sufficient protein bait and hormone stimulation through the complementary formation of three steps, so that the breeding potential of the Yunnan bare lipsticks is maximally stimulated, and the efficient breeding of the Yunnan bare lipsticks is promoted.
Drawings
FIG. 1 is a physiological index diagram of blood of Yunnan Guangxi lip fish cultured by the method of the invention.
FIG. 2 is a serum character diagram of Yunan Guangyi Dianchi cultured by the method of the present invention.
Detailed Description
The technical solution of the present invention is further limited by the following embodiments, but the scope of the claims is not limited to the description.
Example 1
An artificial breeding method of acrossocheilus yunnanensis comprises the following steps:
(1) improvement of spawning pool
Injecting water into the spawning inducing pool until the pool is full, adding ozone into the water, covering the surface of the pool with a plastic film, standing for 50min to ensure that the ozone can fully kill pathogenic microorganisms in the pool, then emptying the water in the pool, and washing for 2 times with clear water; laying 3 cm-thick gravel at the bottom of the induced spawning pond, so that the water quality in the water can be kept clean for a long time, and adding water to ensure that the water depth in the pond is 70 cm; the manufacturing method of the sand gravel comprises the following steps: mixing river sand and celestite, pulverizing into granules, washing with clear water at flow rate of 1.5m/s for 3min, draining, heating to 150 deg.C under 0.1 standard atmospheric pressure, microwave treating, naturally cooling to room temperature, washing with clear water for 10min, and oven drying; wherein the concentration of the ozone in the aqueous solution is 0.5mg/L, so that the sterilization effect of the ozone is ensured; the mass ratio of the river sand to the celestite is 10:1, so that gravel mineral components are balanced, and the pool water can form various ionic solutions conveniently; the microwave treatment conditions are as follows: the power is 1200W, and the time is 10 min; the crushing granularity of the river sand and celestite is 0.8cm, and the uniformity and the adsorption surface of gravel are improved.
(2) Stocking parent fish
Putting the sexually mature parent fish into an induced spawning pond, feeding the biologically extracted feed for 1 week; the preparation method of the biological extraction feed comprises the following steps: silkworm chrysalis, locust, bamboo worms and red earthworms are mixed and pulped according to the mass ratio of 1:1:1:5, the mixture is filtered by 200-mesh gauze, and the obtained filtrate is dried under reduced pressure at the temperature of 20 ℃ until the water content is 30 percent for later use; drying the obtained filter residue at 30 ℃ under reduced pressure until the water content is 5%, and then carrying out superfine grinding on the filter residue for later use; mixing the dried filtrate and the superfine crushed substance of the filter residue, and granulating to obtain the biological extraction feed; wherein, the granularity of the biological extraction feed is 40 meshes, so as to be beneficial to the digestion and absorption of the Yunan Ophioglossus.
(3) Spawning induction of parent fish
Controlling the water temperature at 20 ℃ and the dissolved oxygen to be more than 9mg/L, injecting an oxytocic at the abdominal part of the parent fish without scales, wherein the injection time interval is 5 hours, and 30 percent of the oxytocic is injected for the first time, and 30 percent of the oxytocic is injected for the second time and 40 percent of the oxytocic is injected for the third time; the oxytocic comprises the following components in dose: luteinizing release hormone analogue 6ug/kg + altanone 4mg/kg + reserpine 1 mg/kg.
Example 2
An artificial breeding method of acrossocheilus yunnanensis comprises the following steps:
(1) improvement of spawning pool
Injecting water into the spawning inducing pool until the pool is full, adding ozone into the water, covering the surface of the pool with a plastic film, standing for 60min to ensure that the ozone can fully kill pathogenic microorganisms in the pool, then emptying the water in the pool, and washing for 4 times with clear water; laying gravel with the thickness of 5cm at the bottom of the induced spawning pool, so that the water quality in the water can be kept clean for a long time, and adding water to ensure that the water depth in the pool is 80 cm; the manufacturing method of the sand gravel comprises the following steps: mixing river sand and celestite, pulverizing into granules, washing with clear water at flow rate of 2m/s for 5min, draining, heating to 200 deg.C under 0.3 standard atmospheric pressure, microwave treating, naturally cooling to room temperature, washing with clear water for 20min, and oven drying; wherein the concentration of the ozone in the aqueous solution is 0.8mg/L, so that the sterilization effect of the ozone is ensured; the mass ratio of the river sand to the celestite is 10:1, so that gravel mineral components are balanced, and the pool water can form various ionic solutions conveniently; the microwave treatment conditions are as follows: the power is 1500W, and the time is 15 min; the crushing granularity of the river sand and celestite is 1.3cm, and the uniformity and the adsorption surface of gravel are improved.
(2) Stocking parent fish
Putting the sexually mature parent fish into an induced spawning pond, feeding biological extract feed, and feeding for 2 weeks; the preparation method of the biological extraction feed comprises the following steps: silkworm chrysalis, locust, bamboo worms and red earthworms are mixed and pulped according to the mass ratio of 1:1:1:5, filtered by 300-mesh gauze, and the obtained filtrate is dried under reduced pressure at 25 ℃ until the water content is 35% for later use; drying the obtained filter residue at 35 deg.C under reduced pressure until the water content is 8%, and micronizing the filter residue for use; mixing the dried filtrate and the superfine crushed substance of the filter residue, and granulating to obtain the biological extraction feed; wherein the granularity of the biological extraction feed is 60 meshes, so as to be beneficial to the digestion and absorption of the Yunan Ophioglossus.
(3) Spawning induction of parent fish
Controlling the water temperature at 23 ℃ and the dissolved oxygen to be more than 9mg/L, injecting an oxytocic at the abdominal part of the parent fish without scales, wherein the injection time interval is 8 hours, and 30 percent of the oxytocic is injected for the first time, and 30 percent of the oxytocic is injected for the second time and 40 percent of the oxytocic is injected for the third time; the oxytocic comprises the following components in dose: luteinizing release hormone analogue 6ug/kg + altanone 4mg/kg + reserpine 1 mg/kg.
Example 3
An artificial breeding method of acrossocheilus yunnanensis comprises the following steps:
(1) improvement of spawning pool
Injecting water into the spawning inducing pool until the pool is full, adding ozone into the water, covering the surface of the pool with a plastic film, standing for 55min to ensure that the ozone can fully kill pathogenic microorganisms in the pool, then emptying the water in the pool, and washing for 3 times with clear water; laying 3-54cm thick gravel at the bottom of the induced spawning pond to keep the water quality clean for a long time, and adding water to make the water depth in the pond be 75 cm; the manufacturing method of the sand gravel comprises the following steps: mixing river sand and celestite, pulverizing into granules, washing with clear water at flow rate of 1.5m/s for 5min, draining, heating to 150 deg.C under 0.3 standard atmospheric pressure, microwave treating, naturally cooling to room temperature, washing with clear water for 20min, and oven drying; wherein the concentration of the ozone in the aqueous solution is 0.8mg/L, so that the sterilization effect of the ozone is ensured; the mass ratio of the river sand to the celestite is 10:1, so that gravel mineral components are balanced, and the pool water can form ion solution with rich types conveniently; the microwave treatment conditions are as follows: the power is 1200W, and the time is 15 min; the crushing granularity of the river sand and celestite is 1.3cm, and the uniformity and the adsorption surface of gravel are improved.
(2) Stocking parent fish
Putting the sexually mature parent fish into an induced spawning pond, feeding biological extract feed, and feeding for 2 weeks; the preparation method of the biological extraction feed comprises the following steps: silkworm chrysalis, locust, bamboo worms and red earthworms are mixed and pulped according to the mass ratio of 1:1:1:5, filtered by 300-mesh gauze, and the obtained filtrate is dried under reduced pressure at the temperature of 20 ℃ until the water content is 30% for later use; drying the obtained filter residue at 35 deg.C under reduced pressure until the water content is 8%, and micronizing the filter residue for use; mixing the dried filtrate and the superfine crushed substance of the filter residue, and granulating to obtain the biological extraction feed; wherein, the granularity of the biological extraction feed is 40 meshes, so as to be beneficial to the digestion and absorption of the Yunan Ophioglossus.
(3) Spawning induction of parent fish
Controlling the water temperature at 23 ℃ and the dissolved oxygen to be more than 9mg/L, injecting an oxytocic at the abdominal part of the parent fish without scales, wherein the injection time interval is 8 hours, and 30 percent of the oxytocic is injected for the first time, and 30 percent of the oxytocic is injected for the second time and 40 percent of the oxytocic is injected for the third time; the oxytocic comprises the following components in dose: luteinizing release hormone analogue 6ug/kg + altanone 4mg/kg + reserpine 1 mg/kg.
Comparative example 1
The difference from example 1 is that the gravel used at the bottom of the pond in step (1) is river sand, and the other operations are the same.
Comparative example 2
The difference from example 2 is that in step (2), the parent fish is directly thrown into the spawning induction pond and directly subjected to the third step without using the bio-extract feed of the present invention for adaptive feeding; the other operations are the same.
Comparative example 3
The difference from example 3 is that, in step (2), the dose of the oxytocin is as follows: luteinizing release hormone analogue 5ug/kg + delosone 5 mg/kg; and (4) other operations.
Test example 1
In a Yunnan Guangyi fingerling cultivation base in Guizhou province, Qujing City, Yunnan province, 600 pieces of selectively mature Yunnan Guangyi fingerlings are randomly divided into 6 groups, and each group comprises 100 pieces, namely an experimental group 1-3 and a control group 1-3; wherein the experimental group is operated according to the examples 1-3, the control group is operated according to the comparative examples 1-3, and each group is fed in a pond without mutual interference; and after the experiment is finished, measuring the blood indexes and fertilization and spawning conditions of each group of Yunan liptopilia.
Results of the experiment
1. The physiological index of the blood of Yunan Ophioglossus is shown in figure 1 (lymphocyte × 10)4(ii) a Hemoglobin g/L), as can be seen from the figure, the lymphocytes and the red blood cells of the experimental group 1-3 Yunan Guangshi Gai are higher than those of the control group 1-3, and obviously, the Yunan Guangshi Gai of the experimental group is caused by the control group in response to the environmental adverse stimulus.
2. Serum character of Yunan Guangyi Dianchi (phosphorus mmol/L; iron mmol/L)
Phosphorus is a major mineral element necessary for the growth of fish bodies, is one of main components of bones, teeth and scales, and has important physiological functions in the aspects of maintaining the acid-base balance of the fish bodies, regulating osmotic pressure, forming coenzyme and organophosphorus compounds, promoting blood coagulation, muscle contraction, neurotransmission, maintaining cell integrity and the like; iron participates in the metabolism and immunologic function of the substances in the fish body; as can be seen from the figure 2, the phosphorus and iron contents in the serum of the Yunan Guangxi Gaertn fishes in the experiment groups 1-3 are maintained at higher levels, while the phosphorus and iron contents in the serum of the control group are lower, so that the serum indexes in the blood of the experiment groups are obviously improved by the method, the metabolism of b vitamins can be more fully promoted, the physiological function in the fish body is improved, and the reproductive performance of the Yunan Guangxi Gaertn fishes is further improved.
3. Statistics of spawning induction rate, fertility rate and hatching rate of acrossocheilus fasciatus
| | Experiment | 2 | Experiment | 3 | Control group | 1 | |
|
|
| Catalytic yield | 86.3％ | 85.7％ | 88.3％ | 65.74％ | 63.07％ | 64.31％ | |||
| Fertilization rate | 88.5％ | 91.3％ | 89.47％ | 71.35％ | 72.19％ | 73.44％ | |||
| Hatching rate | 75.3％ | 76.31％ | 73.84％ | 55.86％ | 61.87 | 59.81％ |
As can be seen from the table, according to the method, the spawning induction rate of the Yunnan bare lipped fish exceeds 86.3%, the fertilization rate exceeds 88.5%, the hatching rate exceeds 75.3%, the results are higher than those of a control group, and the effect is obvious.
It should be noted that the above examples and experimental examples are only for further illustration and understanding of the technical solutions of the present invention, and should not be construed as further limitations of the technical solutions of the present invention, and the invention with insubstantial features and significant improvements made by those skilled in the art still falls within the scope of protection of the present invention.
Claims (5)
1. An artificial breeding method of acrossocheilus yunnanensis is characterized by comprising the following steps:
(1) improvement of spawning pool
Injecting water into the spawning inducing pool until the pool is full, adding ozone into the water, covering the surface of the pool with a plastic film, standing for 50-60min, then emptying the water in the pool, and washing for 2-4 times with clear water; laying 3-5cm thick gravel at the bottom of the spawning inducing pool, and adding water to make the water depth in the pool be 70-80 cm; the manufacturing method of the sand gravel comprises the following steps: mixing and crushing river sand and celestite into particles, flushing the particles for 3 to 5min by clear water with the flow rate of 1.5 to 2m/s, draining the particles, heating the particles to the temperature of 150 ℃ and 200 ℃ under the standard atmospheric pressure of 0.1 to 0.3, performing microwave treatment, naturally cooling the particles to the normal temperature, flushing the particles for 10 to 20min by the clear water, and drying the particles; the mass ratio of the river sand to the celestite is 10: 1; the crushed particle size of the river sand and celestite is 0.8-1.3 cm;
(2) stocking parent fish
Putting the sexually mature parent fish into an induced spawning pond, feeding biological extract feed for 1-2 weeks; the preparation method of the biological extraction feed comprises the following steps: mixing and pulping silkworm chrysalis, locust, bamboo worms and red earthworms according to the mass ratio of 1:1:1:5, filtering by 200-mesh gauze, and drying the obtained filtrate at 20-25 ℃ under reduced pressure until the water content is 30-35% for later use; drying the obtained filter residue at 30-35 deg.C under reduced pressure until the water content is 5-8%, and micronizing the filter residue for use; mixing the dried filtrate and the superfine crushed substances of the filter residue, and granulating to obtain the biological extraction feed;
(3) spawning induction of parent fish
Controlling the water temperature at 20-23 ℃ and the dissolved oxygen to be more than 9mg/L, injecting an oxytocic at the abdominal part of the parent fish without scales, injecting 30% of the oxytocic for the first time and 30% of the oxytocic for the second time and injecting 40% of the oxytocic for the third time, wherein the injection time interval is 5-8 hours.
2. The artificial breeding method of acrossocheilus yunnanensis as claimed in claim 1, wherein the concentration of ozone in the aqueous solution is 0.5-0.8 mg/L.
3. The artificial breeding method of acrossocheilus yunnanensis as claimed in claim 1, wherein the microwave treatment conditions are as follows: the power is 1200W-1500W, and the time is 10-15 min.
4. The artificial breeding method of acrossocheilus yunnanensis as claimed in claim 1, wherein the granularity of the biological extract feed is 40-60 meshes.
5. The artificial breeding method of acrossocheilus yunnanensis as claimed in claim 1, wherein the oxytocic is prepared from the following raw materials in parts by weight: luteinizing releasing hormone 6ug/kg + altanone 4mg/kg + reserpine 1 mg/kg.
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| CN117296756A (en) * | 2023-10-31 | 2023-12-29 | 珠江水资源保护科学研究所 | Artificial propagation method for Alcheilus longifolia |
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| CN101053320A (en) * | 2007-05-09 | 2007-10-17 | 广东省大亚湾水产试验中心 | Factory culturing method for grouper |
| CN103098737A (en) * | 2013-02-26 | 2013-05-15 | 浙江师范大学 | Manpower hasten parturition method of acrossocheilus fasciatus |
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| DE1607274A1 (en) * | 1966-12-29 | 1970-06-04 | Max Planck Gesellschaft | Process for the reproduction, rearing and keeping of fresh and seawater animals, in particular farm fish, in large quantities, but in a confined space |
| CN101053320A (en) * | 2007-05-09 | 2007-10-17 | 广东省大亚湾水产试验中心 | Factory culturing method for grouper |
| CN103098737A (en) * | 2013-02-26 | 2013-05-15 | 浙江师范大学 | Manpower hasten parturition method of acrossocheilus fasciatus |
| CN103444610A (en) * | 2013-09-23 | 2013-12-18 | 云南省水产技术推广站 | Artificial propagation method for acrossocheilus yunnanensis |
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