CN111278852B - 重组欧氏杆菌天冬酰胺酶的生产方法 - Google Patents

重组欧氏杆菌天冬酰胺酶的生产方法 Download PDF

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CN111278852B
CN111278852B CN201880069961.4A CN201880069961A CN111278852B CN 111278852 B CN111278852 B CN 111278852B CN 201880069961 A CN201880069961 A CN 201880069961A CN 111278852 B CN111278852 B CN 111278852B
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asparaginase
expression
pseudomonas
protein
recombinant
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CN111278852A (zh
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R·J·科尔曼
T·布鲁克
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Phonex Corp
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Phonex Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01001—Asparaginase (3.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C12R2001/39—Pseudomonas fluorescens

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201880069961.4A 2017-10-27 2018-10-17 重组欧氏杆菌天冬酰胺酶的生产方法 Active CN111278852B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762578305P 2017-10-27 2017-10-27
US62/578,305 2017-10-27
PCT/US2018/056374 WO2019083793A1 (en) 2017-10-27 2018-10-17 Method for production of recombinant erwinia asparaginase

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CN111278852A CN111278852A (zh) 2020-06-12
CN111278852B true CN111278852B (zh) 2024-07-16

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Country Link
US (2) US10787671B2 (https=)
EP (1) EP3700921A4 (https=)
JP (2) JP7784804B2 (https=)
CN (1) CN111278852B (https=)
AU (1) AU2018354067B2 (https=)
SG (1) SG11202002943PA (https=)
WO (1) WO2019083793A1 (https=)

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WO2019083794A1 (en) * 2017-10-27 2019-05-02 Pfenex Inc. PROCESS FOR PRODUCING EAR ASPARAGINASE. RECOMBINED COLI
JP7784804B2 (ja) * 2017-10-27 2025-12-12 フェネックス インク． 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法
EP3747461A1 (en) * 2019-06-04 2020-12-09 Kyon Biotech AG Asparaginase-based cancer therapy
GB201912020D0 (en) * 2019-08-21 2019-10-02 Porton Biopharma Ltd Therapeutic Conjugate
JP2022553399A (ja) * 2019-10-25 2022-12-22 ジャズ ファーマシューティカルズ アイルランド リミテッド 組換えｌ－アスパラギナーゼ
CN112341533A (zh) * 2020-11-20 2021-02-09 东北师范大学 无标签人半乳糖凝集素13及其制备方法和应用
US20220227805A1 (en) 2020-12-23 2022-07-21 Jazz Pharmaceuticals Ireland Ltd. Methods of purifying charge-shielded fusion proteins
WO2022211829A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase
WO2024015529A2 (en) 2022-07-14 2024-01-18 Jazz Pharmaceuticals Ireland Ltd. Combination therapies involving l-asparaginase
WO2024186259A1 (en) * 2023-03-07 2024-09-12 Karyolaimos Alexandros A method for enhancing recombinant production in a production host, an expression system, a library, a platform system, a production strain, an e. coli strain, a kit of parts, a method of using the platform system and a computer program, related to the method
EP4689147A2 (en) 2023-04-05 2026-02-11 Primrose Bio, Inc. Methods and compositions for nucleic acid synthesis
CN117165564B (zh) * 2023-08-16 2025-01-28 云南师范大学 一种ii型l-天冬酰胺酶及其应用

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CN101031655A (zh) * 2004-07-26 2007-09-05 陶氏环球技术公司 通过株工程改进蛋白表达的方法
CN102869778A (zh) * 2010-03-30 2013-01-09 菲尼克斯公司 重组毒素蛋白的高水平表达

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CN101031655A (zh) * 2004-07-26 2007-09-05 陶氏环球技术公司 通过株工程改进蛋白表达的方法
CN102869778A (zh) * 2010-03-30 2013-01-09 菲尼克斯公司 重组毒素蛋白的高水平表达

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US20210032640A1 (en) 2021-02-04
JP7784804B2 (ja) 2025-12-12
US10787671B2 (en) 2020-09-29
US11377661B2 (en) 2022-07-05
WO2019083793A1 (en) 2019-05-02
JP2021500896A (ja) 2021-01-14
AU2018354067A1 (en) 2020-06-04
US20190127742A1 (en) 2019-05-02
CN111278852A (zh) 2020-06-12
EP3700921A4 (en) 2021-12-15
AU2018354067B2 (en) 2023-03-30
EP3700921A1 (en) 2020-09-02
SG11202002943PA (en) 2020-05-28
JP2023123512A (ja) 2023-09-05

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