JP7784804B2 - 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法 - Google Patents

組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法

Info

Publication number
JP7784804B2
JP7784804B2 JP2020523432A JP2020523432A JP7784804B2 JP 7784804 B2 JP7784804 B2 JP 7784804B2 JP 2020523432 A JP2020523432 A JP 2020523432A JP 2020523432 A JP2020523432 A JP 2020523432A JP 7784804 B2 JP7784804 B2 JP 7784804B2
Authority
JP
Japan
Prior art keywords
asparaginase
protein
expression
liter
crisantaspase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2020523432A
Other languages
English (en)
Japanese (ja)
Other versions
JP2021500896A5 (https=
JP2021500896A (ja
Inventor
ラッセル・ジェイ・コールマン
トーベン・ブルック
Original Assignee
フェネックス インク．
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by フェネックス インク． filed Critical フェネックス インク．
Publication of JP2021500896A publication Critical patent/JP2021500896A/ja
Publication of JP2021500896A5 publication Critical patent/JP2021500896A5/ja
Priority to JP2023093346A priority Critical patent/JP2023123512A/ja
Application granted granted Critical
Publication of JP7784804B2 publication Critical patent/JP7784804B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01001—Asparaginase (3.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C12R2001/39—Pseudomonas fluorescens

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2020523432A 2017-10-27 2018-10-17 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法 Active JP7784804B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023093346A JP2023123512A (ja) 2017-10-27 2023-06-06 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762578305P 2017-10-27 2017-10-27
US62/578,305 2017-10-27
PCT/US2018/056374 WO2019083793A1 (en) 2017-10-27 2018-10-17 Method for production of recombinant erwinia asparaginase

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023093346A Division JP2023123512A (ja) 2017-10-27 2023-06-06 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法

Publications (3)

Publication Number Publication Date
JP2021500896A JP2021500896A (ja) 2021-01-14
JP2021500896A5 JP2021500896A5 (https=) 2021-11-18
JP7784804B2 true JP7784804B2 (ja) 2025-12-12

Family

ID=66242732

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2020523432A Active JP7784804B2 (ja) 2017-10-27 2018-10-17 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法
JP2023093346A Withdrawn JP2023123512A (ja) 2017-10-27 2023-06-06 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2023093346A Withdrawn JP2023123512A (ja) 2017-10-27 2023-06-06 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法

Country Status (7)

Country Link
US (2) US10787671B2 (https=)
EP (1) EP3700921A4 (https=)
JP (2) JP7784804B2 (https=)
CN (1) CN111278852B (https=)
AU (1) AU2018354067B2 (https=)
SG (1) SG11202002943PA (https=)
WO (1) WO2019083793A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019083794A1 (en) * 2017-10-27 2019-05-02 Pfenex Inc. PROCESS FOR PRODUCING EAR ASPARAGINASE. RECOMBINED COLI
JP7784804B2 (ja) * 2017-10-27 2025-12-12 フェネックス インク． 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法
EP3747461A1 (en) * 2019-06-04 2020-12-09 Kyon Biotech AG Asparaginase-based cancer therapy
GB201912020D0 (en) * 2019-08-21 2019-10-02 Porton Biopharma Ltd Therapeutic Conjugate
JP2022553399A (ja) * 2019-10-25 2022-12-22 ジャズ ファーマシューティカルズ アイルランド リミテッド 組換えｌ－アスパラギナーゼ
CN112341533A (zh) * 2020-11-20 2021-02-09 东北师范大学 无标签人半乳糖凝集素13及其制备方法和应用
US20220227805A1 (en) 2020-12-23 2022-07-21 Jazz Pharmaceuticals Ireland Ltd. Methods of purifying charge-shielded fusion proteins
WO2022211829A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase
WO2024015529A2 (en) 2022-07-14 2024-01-18 Jazz Pharmaceuticals Ireland Ltd. Combination therapies involving l-asparaginase
WO2024186259A1 (en) * 2023-03-07 2024-09-12 Karyolaimos Alexandros A method for enhancing recombinant production in a production host, an expression system, a library, a platform system, a production strain, an e. coli strain, a kit of parts, a method of using the platform system and a computer program, related to the method
EP4689147A2 (en) 2023-04-05 2026-02-11 Primrose Bio, Inc. Methods and compositions for nucleic acid synthesis
CN117165564B (zh) * 2023-08-16 2025-01-28 云南师范大学 一种ii型l-天冬酰胺酶及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010517532A (ja) 2007-01-31 2010-05-27 ダウ グローバル テクノロジーズ インコーポレイティド 発現上昇のための細菌リーダー配列
JP2010524506A (ja) 2007-04-27 2010-07-22 ダウ グローバル テクノロジーズ インコーポレイティド 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および／または質が改善されている特定の株を同定する方法
JP2012532185A (ja) 2009-07-06 2012-12-13 アリゼ ファーマ トゥー ペグ化されたｌ−アスパラギナーゼ
JP2013529064A (ja) 2010-03-30 2013-07-18 フェニックス インク． 組み換え型毒素タンパク質の高レベルの発現

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US4755465A (en) 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US5281532A (en) 1983-07-27 1994-01-25 Mycogen Corporation Pseudomas hosts transformed with bacillus endotoxin genes
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
US4695455A (en) 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
GB8517071D0 (en) 1985-07-05 1985-08-14 Hoffmann La Roche Gram-positive expression control sequence
GB8519753D0 (en) * 1985-08-06 1985-09-11 Health Lab Service Board Production of 1-asparaginase
US5128130A (en) 1988-01-22 1992-07-07 Mycogen Corporation Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens
US5055294A (en) 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
US5169760A (en) 1989-07-27 1992-12-08 Mycogen Corporation Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters
GB9017002D0 (en) 1990-08-02 1990-09-19 Health Lab Service Board Improved method for the purification of erwina l-asparaginase
US9453251B2 (en) * 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
CA2545610C (en) 2003-11-19 2014-03-25 Dow Global Technolgies Inc. Auxotrophic pseudomonas fluorescens bacteria for recombinant protein expression
AU2004317306B2 (en) 2003-11-21 2010-09-02 Pelican Technology Holdings, Inc. Improved expression systems with Sec-system secretion
US8603824B2 (en) 2004-07-26 2013-12-10 Pfenex, Inc. Process for improved protein expression by strain engineering
EP2021489A2 (en) 2006-05-30 2009-02-11 Dow Global Technologies Inc. Codon optimization method
MX2009000284A (es) * 2006-06-30 2009-01-26 Enzon Pharmaceuticals Inc Hospedero recombinante para produccion de l-asparaginasa ii.
WO2008128975A1 (en) 2007-04-20 2008-10-30 Dsm Ip Assets B.V. Novel asparaginases and uses thereof
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
PL2451486T5 (pl) * 2009-07-06 2023-05-15 Jazz Pharmaceuticals II S.A.S. Pegylowana l-asparaginaza
GB0917647D0 (en) 2009-10-08 2009-11-25 Glaxosmithkline Biolog Sa Expression system
RU2441914C1 (ru) * 2010-10-06 2012-02-10 Учреждение Российской академии медицинских наук Научно-исследовательский институт биомедицинской химии имени В.Н. Ореховича РАМН (ИБМХ РАМН) Способ получения субстанции рекомбинантной l-аспарагиназы erwinia carotovora
BR102014000585B1 (pt) 2014-01-10 2021-01-12 Coppe/Ufrj-Instituto Alberto Luiz Coimbra De Pós-Graduação l-asparaginase recombinante de zymomonas
KR102092225B1 (ko) 2014-04-30 2020-03-23 주식회사 엘지화학 고효율 분비능을 가지는 단백질 분비 인자 및 이를 포함하는 발현 벡터
JP6817939B2 (ja) 2014-12-01 2021-01-20 フェネックス インク． ペプチド産生のための融合パートナー
JP7784804B2 (ja) * 2017-10-27 2025-12-12 フェネックス インク． 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法
AU2018354069A1 (en) 2017-10-27 2020-06-11 Pelican Technology Holdings, Inc. Bacterial leader sequences for periplasmic protein expression
WO2019083794A1 (en) 2017-10-27 2019-05-02 Pfenex Inc. PROCESS FOR PRODUCING EAR ASPARAGINASE. RECOMBINED COLI

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010517532A (ja) 2007-01-31 2010-05-27 ダウ グローバル テクノロジーズ インコーポレイティド 発現上昇のための細菌リーダー配列
JP2010524506A (ja) 2007-04-27 2010-07-22 ダウ グローバル テクノロジーズ インコーポレイティド 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および／または質が改善されている特定の株を同定する方法
JP2012532185A (ja) 2009-07-06 2012-12-13 アリゼ ファーマ トゥー ペグ化されたｌ−アスパラギナーゼ
JP2013529064A (ja) 2010-03-30 2013-07-18 フェニックス インク． 組み換え型毒素タンパク質の高レベルの発現

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Diane M. Retallack et al., Reliable protein production in a Pseudomonas fluorescens expression system, Protein expression and purification, ２０１１年９月２４日, Vol.81, p.157-165

Also Published As

Publication number Publication date
US20210032640A1 (en) 2021-02-04
US10787671B2 (en) 2020-09-29
US11377661B2 (en) 2022-07-05
CN111278852B (zh) 2024-07-16
WO2019083793A1 (en) 2019-05-02
JP2021500896A (ja) 2021-01-14
AU2018354067A1 (en) 2020-06-04
US20190127742A1 (en) 2019-05-02
CN111278852A (zh) 2020-06-12
EP3700921A4 (en) 2021-12-15
AU2018354067B2 (en) 2023-03-30
EP3700921A1 (en) 2020-09-02
SG11202002943PA (en) 2020-05-28
JP2023123512A (ja) 2023-09-05

Similar Documents

Publication Publication Date Title
JP7784804B2 (ja) 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法
US11046964B2 (en) Method for production of recombinant E. coli asparaginase
EP2553102B1 (en) High level expression of recombinant toxin proteins
EP0244042B1 (en) Secretory signal selection vectors for extracellular protein synthesis in bacilli
AU2010201410B2 (en) High level expression of recombinant CRM197
KR20190121331A (ko) 바실러스 리체니포르미스에서 단백질 생산을 증가시키기 위한 조성물 및 방법
US20230100757A1 (en) Bacterial hosts for recombinant protein expression
CN111372941A (zh) 用于周质蛋白质表达的细菌前导序列
JP5828634B2 (ja) 組換え微生物を用いたタンパク質の製造方法
WO2024123627A1 (en) Methods for expression of fusion-free bovine ultralong cdr3 scaffold

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211006

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20211006

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220830

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20220831

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20221129

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20230207

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230606

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20230630

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20230804

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20240502

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20250217

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20250528

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250904

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20251202

R150 Certificate of patent or registration of utility model

Ref document number: 7784804

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150