CN111265574A - Preparation method of anti-alcohol enteric coated lipid nanoparticle oral liquid - Google Patents
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Abstract
The invention provides a preparation method of an anti-alcoholism enteric-coated lipid nanoparticle oral liquid, belonging to the technical field of bioengineering and comprising the following steps: s1: drying semen Hoveniae, flos Puerariae Lobatae, and passion fruit, pulverizing, and sieving; s2: soaking semen Hoveniae powder and distilled water, decocting with slow fire, and filtering to obtain semen Hoveniae extractive solution; preparing flos Puerariae Lobatae extract and Passiflora edulis extract by the same method; s3: mixing the three solutions; s4: weighing glyceryl monostearate, soybean lecithin and absolute ethyl alcohol, adding the mixed extract, and stirring to obtain an oil phase; s5: 1.5% poloxamer F68 was heated for use as the aqueous phase; injecting the oil phase into the water phase, stirring, concentrating, stirring in ice bath, and centrifuging to obtain lipid nanoparticles; s6: injecting the coating solution into the lipid nanoparticles, and stirring. The anti-alcoholism enteric-coated lipid nanoparticle oral liquid prepared by the invention can enable the nanoparticle medicine to completely pass through the stomach until the nanoparticle medicine is smoothly dissolved in the intestine, has ideal anti-alcoholism effect, does not stimulate human bodies, and has quick drug effect.
Description
Technical Field
The invention relates to the technical field of bioengineering, in particular to a preparation method of an anti-alcoholism enteric-coated lipid nanoparticle oral liquid.
Background
When the concentration of ethanol in blood is 0.05-0.1%, people become hazy and smoothly slightly drunk; when the concentration reaches 0.3%, people have unclear mouth and teeth and stumbling gait, which is what we often say as drunkenness. In general, acetaldehyde dehydrogenase is not completely decomposed into acetic acid because of the lack of acetaldehyde dehydrogenase in human body, but acetaldehyde remains in the body, which causes intoxication symptoms such as nausea, vomiting, coma and discomfort after drinking, and excessive drinking may damage liver, resulting in chronic alcoholism.
At present, a part of people mainly use a civil soil method for dispelling the effects of alcohol, so that the scientific basis is not too much, the effect is not obvious, the alcohol-dispelling medicines in the market generally adopt capsule shells, the dissolving time is long, the absorption is not easy, and the alcohol-dispelling effect cannot be exerted in time.
The patent with the publication number of CN101756312B discloses a preparation method of a microencapsulated drink of a Nanguo pear liver-protecting anti-alcoholism agent, which is specially used for Nanguo pear fruits and provides a theoretical basis for the quantitative production of alcohol and acetaldehyde dehydrogenase in the Nanguo pear liver-protecting anti-alcoholism drink. 1. The process flow comprises the following steps: selecting raw materials → cleaning → cutting block → soaking in ice bath → grinding in ice bath → homogenizing in ice bath → leaching at 0-4 deg.C for 1h → 1-2 deg.C, centrifuging at 4000rpm for 15min → freeze concentrating → preparing enteric microcapsule of mixed concentrated solution of ethanol, acetaldehyde dehydrogenase, etc → blending beverage according to required package. The beverage has low bioavailability and poor hangover alleviating effect.
An oral medicine for relieving alcoholism is disclosed in patent No. CN102552547B, in particular to a prescription of an oral medicine for relieving alcoholism prepared by taking traditional Chinese medicines and vitamins as raw materials and a preparation process thereof. Adding water into 49-50% of pueraria lobata flower and 49-50% of semen hoveniae, adding 0.5-1% of tea polyphenol and 0.5-1% of L-glutamine, granulating after spray drying, adding water, decocting for three times, wherein the water addition amount is respectively 10 times, 8 times and 8 times of the weight of the medicinal materials, the decocting time is 0.5 hour, combining obtained liquid medicines, concentrating to the relative density of 1.1, spray drying into fine powder, adding the tea polyphenol, the L-glutamine and a proper amount of auxiliary materials, and granulating. The oral medicine can dissolve in stomach, reduce drug effect, and stimulate human body.
Disclosure of Invention
In view of the above, the invention provides a preparation method of an anti-alcoholism enteric-coated lipid nanoparticle oral liquid.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a preparation method of an anti-alcoholism enteric-coated lipid nanoparticle oral liquid comprises the following steps:
s1: taking fresh, rotten, mildewed and worm-eaten-free hovenia dulcis thunb, pueraria flower and passion fruit, cleaning with clear water, airing, and cutting the passion fruit into blocks and removing seeds; vacuum drying until the water content is lower than 8%, pulverizing with pulverizer, and sieving with 80-100 mesh sieve to obtain semen Hoveniae powder, flos Puerariae Lobatae powder and passion fruit powder;
s2: taking the semen hoveniae powder and the distilled water obtained in the step S1, soaking, decocting with slow fire for 1-1.5h, filtering to obtain filtrate, then adding the same mass of distilled water into the filter residue, decocting with slow fire for 1-1.5h, filtering to obtain filtrate, and combining the two filtrates to obtain semen hoveniae extract; preparing flos Puerariae Lobatae extract and Passiflora edulis extract according to the same method;
s3: uniformly mixing the hovenia dulcis thunb extract, the pueraria flower extract and the passion fruit extract in the step S2 for later use;
s4: weighing glyceryl monostearate, soybean lecithin and absolute ethyl alcohol, and stirring uniformly in a water bath; adding the mixed extracting solution prepared in the step S3, and uniformly stirring to be used as an oil phase;
s5: heating 1.5% poloxamer F68 to 75-80 ℃ as the aqueous phase; injecting the oil phase in the step S4 into the water phase, stirring, concentrating, rapidly dispersing the system into sterile ice water, stirring in an ice bath, and centrifuging to obtain solid lipid nanoparticles;
s6: weighing medicinal polyacrylic resin to prepare 10mg/ml-20mg/ml enteric coating, injecting the coating solution into the lipid nanoparticles in the step S5 by using a sterile needle tube, stirring at constant temperature of 30-35 ℃ for 0.5h, and completely volatilizing ethanol to obtain the enteric coating lipid nanoparticle oral liquid.
Further, in step S1, the vacuum drying conditions are as follows: 100-.
Further, in step S2, the weight ratio of hovenia dulcis thunb powder is: distilled water, arrowroot flour: water, passion fruit powder: the water is 1:8-1: 9.
Further, in step S3, the volume ratio of 1:1:1-1:2:3= hovenia dulcis thunb extract: and (3) extracting the pueraria flower: passion fruit extract.
Further, in step S4, the mass ratio of 1:2 to 1:4= glyceryl monostearate: soybean lecithin in a mass ratio of 1:60-1:80= (glyceryl monostearate and soybean lecithin): anhydrous ethanol.
Further, in step S5, the volume ratio is 1:3-1:4= oil phase: 1.5% poloxamer F68.
Further, in the step S5, the oil phase is injected into the water phase, the temperature is kept at 75-80 ℃, the stirring is carried out at 1000r/min-1500r/min until the system is concentrated to 1/4-1/3 of the total volume of the original water phase and oil phase, then the system is rapidly dispersed into sterile ice water with the volume of 2-3 times of the original volume, the stirring is carried out in an ice bath for 2h-2.5h, and the centrifugation is carried out at 4000r/min-5000r/min for 10min-15min, so as to obtain the solid lipid nanoparticles.
Along with the continuous improvement of the living standard of people and the more and more rational consumption, drinking is an expression form of work interaction, emotion communication, familiarity condensation and leisure enjoyment, and is deeply involved in the aspects of social cultural life. However, drinking alcohol and liver injury are becoming common knowledge of people as smoking and lung injury, and drinking alcohol can cause diseases such as gastric ulcer, fatty liver, alcoholic hepatitis, liver cirrhosis and the like. Traditionally, in the treatment of acute alcoholism, in order to fully separate out the effective components of the traditional Chinese medicinal materials, a water decoction method is usually adopted, but the decoction of the traditional Chinese medicines takes time, the traditional Chinese medicine decoction is inconvenient to carry and store, and the medicine is dissolved in the stomach, so the anti-alcoholism effect is not ideal. For example, patent document CN105535424A discloses a formula for relieving hangover and a preparation method thereof, wherein the preparation method comprises the following steps: 1. 9 medicinal materials of the flower of kudzuvine, the hovenia dulcis thumb, the dwarf lilyturf tuber, the hawthorn, the costustoot, the dried orange peel, the scorched medicated leaven, the codonopsis pilosula and the astragalus root are respectively ground into fine powder for standby application through processes of cleaning, drying and the like under the sterile and dustless conditions; 2. sieving the medicinal powder, and mixing; 3. leaching the mixed medicinal powder in a traditional Chinese medicine decocting machine, collecting juice, removing residue, decocting, and filtering with a medicinal liquid filter; 4. purifying the extract; 5. concentrating the purified extracting solution: the extract is properly concentrated according to the daily dose to obtain the formula for sobering up and dispelling the effects of alcohol.
Flos puerariae lobatae and semen hoveniae are two traditional anti-alcoholism traditional Chinese medicines, according to ancient documents, flos puerariae lobatae can detoxify alcoholism, and semen hoveniae can lose the taste of alcohol. In recent years, the literature reports about the two methods are focused on the influence of single-taste flos puerariae lobatae or semen hoveniae on acute alcoholism. The compatibility of the pueraria flower and the hovenia dulcis thunb is not researched much, and the compatibility of the pueraria flower and the hovenia dulcis thunb with other substances is less researched. The influence of different proportions of compatibility of the pueraria flower hovenia dulcis on the liver function of rats with alcoholic injury is discussed, and the influence of different extraction methods on the curative effect of the medicines of the pueraria flower hovenia dulcis and the compatibility of the pueraria flower hovenia dulcis on the curative effect of the medicines is also discussed in the scientific and technical reports 2012,30(28-29): 65-69.
Compared with the prior art, the invention has the following beneficial effects:
raisin tree seed: sweet taste, mild nature, entering stomach channel, relieving alcoholism, quenching thirst, relieving restlessness, relieving vomit, facilitating urination and defecation, resisting lipid peroxidation, protecting liver, inhibiting central nerve and obviously clearing free radicals in vivo.
And (3) kudzuvine flower: has sweet taste and cool nature, and can be used for treating alcoholic intoxication, dysphoria, thirst, headache, dizziness, abdominal distention, vomiting, acid regurgitation, anorexia, hematemesis, and intestinal wind induced hemorrhage, relieving alcoholic intoxication, activating spleen, and stopping bleeding.
Passion fruit: the product has effects of exciting and strengthening body, diminishing inflammation, relieving pain, promoting blood circulation, strengthening body constitution, nourishing yin, invigorating kidney, reducing blood lipid, lowering blood pressure, refreshing, relieving fatigue, removing toxic substance, caring skin, and enhancing immunity.
The glyceryl monostearate is white waxy flake or bead solid, is insoluble in water, can be dispersed in water by strong oscillation mixing with hot water, and is water-in-oil type emulsifier; soybean lecithin is an essence extracted from soybean and used as emulsifier. Poloxamer is used as an oil-in-water emulsifier.
The enteric coated anti-alcoholism solid lipid nanoparticle is prepared by taking traditional Chinese medicinal plant materials for anti-alcoholism and protecting liver as raw materials, has stronger drug-loading capacity, can effectively improve the solubility, bioavailability and stability of the drug, has sustained-release and targeting effects, almost has no toxicity to organisms, stable physicochemical property and more obvious drug effect, and achieves the aim of quickly relieving alcoholism.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings.
FIG. 1 shows the nanoparticle morphology after soaking in artificial gastric juice for 2 h.
FIG. 2 shows the form of nanoparticles after the artificial intestinal juice of the invention is soaked for 45 min.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to fig. 1-2 of the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the invention, are within the scope of the invention.
Example 1
A preparation method of an anti-alcoholism enteric-coated lipid nanoparticle oral liquid comprises the following steps:
s1: taking fresh, rotten, mildewed and worm-eaten-free hovenia dulcis thunb, pueraria flower and passion fruit, cleaning with clear water, airing, and cutting the passion fruit into blocks and removing seeds; vacuum drying at 45 deg.C under 100pa until the water content is lower than 8%, pulverizing with pulverizer, and sieving with 80 mesh sieve to obtain semen Hoveniae powder, flos Puerariae Lobatae powder and passion fruit powder;
s2: the raw materials comprise semen hoveniae powder in percentage by mass: distilled water =1: 8, soaking the hovenia dulcis thunb powder and the distilled water obtained in the step S1 for decocting for 1h with slow fire, filtering to obtain filtrate, then adding the same mass of distilled water into the filter residue for decocting for 1h with slow fire, filtering to obtain filtrate, and combining the filtrates of the two times to obtain the hovenia dulcis thunb extracting solution; preparing flos Puerariae Lobatae extract and Passiflora edulis extract according to the same method;
s3: uniformly mixing the hovenia dulcis thunb extract, the pueraria flower extract and the passion fruit extract in the step S2 for later use, wherein the volume ratio of the hovenia dulcis thunb extract is as follows: and (3) extracting the pueraria flower: passion fruit extract =1:1: 1;
s4: according to the mass ratio of 1:2= glyceryl monostearate: weighing glycerol monostearate and soybean lecithin, and then mixing the glycerol monostearate and the soybean lecithin in a mass ratio of 1:60= (glycerol monostearate and soybean lecithin): anhydrous ethanol, weighing the anhydrous ethanol, and uniformly stirring the anhydrous ethanol in a water bath at the temperature of 75 ℃; adding the mixed extracting solution prepared in the step S3, and uniformly stirring to be used as an oil phase;
s5: at a volume ratio of 1:3= oil phase in step S4: 1.5% poloxamer F68, weighing oil phase and 1.5% poloxamer F68 separately, heating 1.5% poloxamer F68 to 75 deg.c to obtain water phase; slowly injecting the oil phase into the water phase by using an injector with a No. 5 needle, keeping the temperature at 75 ℃, stirring at 1000r/min until the system is concentrated to 1/4 of the total volume of the original water phase and the oil phase, then quickly dispersing the system into sterile ice water with the volume of 2 times, stirring for 2h in an ice bath, and centrifuging at 4000r/min for 10min to obtain solid lipid nanoparticles;
s6: weighing medicinal polyacrylic resin to prepare 10mg/ml enteric coating, injecting the coating solution into the lipid nanoparticles in the step S5 by using a sterile needle tube, stirring at constant temperature of 30 ℃ for 0.5h, and completely volatilizing ethanol to obtain the enteric coating lipid nanoparticle oral liquid.
Example 2
A preparation method of an anti-alcoholism enteric-coated lipid nanoparticle oral liquid comprises the following steps:
s1: taking fresh, rotten, mildewed and worm-eaten-free hovenia dulcis thunb, pueraria flower and passion fruit, cleaning with clear water, airing, and cutting the passion fruit into blocks and removing seeds; vacuum drying at 120pa and 50 deg.C until water content is less than 8%, pulverizing with pulverizer, and sieving with 90 mesh sieve to obtain semen Hoveniae powder, flos Puerariae Lobatae powder and passion fruit powder;
s2: the raw materials comprise semen hoveniae powder in percentage by mass: distilled water =1: 8, soaking the hovenia dulcis thunb powder and the distilled water obtained in the step S1 for decocting for 1h with slow fire, filtering to obtain filtrate, then adding the same mass of distilled water into the filter residue for decocting for 1h with slow fire, filtering to obtain filtrate, and combining the filtrates of the two times to obtain the hovenia dulcis thunb extracting solution; preparing flos Puerariae Lobatae extract and Passiflora edulis extract according to the same method;
s3: uniformly mixing the hovenia dulcis thunb extract, the pueraria flower extract and the passion fruit extract in the step S2 for later use, wherein the volume ratio of the hovenia dulcis thunb extract is as follows: and (3) extracting the pueraria flower: passion fruit extract =1:2: 3;
s4: according to the mass ratio of 1:3= glyceryl monostearate: weighing glycerol monostearate and soybean lecithin, and then mixing the glycerol monostearate and the soybean lecithin in a mass ratio of 1:70= (glycerol monostearate and soybean lecithin): anhydrous ethanol, weighing the anhydrous ethanol, and uniformly stirring the anhydrous ethanol in a water bath at the temperature of 75 ℃; adding the mixed extracting solution prepared in the step S3, and uniformly stirring to be used as an oil phase;
s5: at a volume ratio of 1:3= oil phase in step S4: 1.5% poloxamer F68, weighing oil phase and 1.5% poloxamer F68 separately, heating 1.5% poloxamer F68 to 75 deg.c to obtain water phase; slowly injecting the oil phase into the water phase by using an injector with a No. 5 needle, keeping the temperature at 75 ℃, stirring at 1500r/min until the system is concentrated to 1/4 of the total volume of the original water phase and the oil phase, then quickly dispersing the system into sterile ice water with the volume of 2 times, stirring for 2h in an ice bath, and centrifuging at 4000r/min for 10min to obtain solid lipid nanoparticles;
s6: weighing medicinal polyacrylic resin to prepare 20mg/ml enteric coating, injecting the coating solution into the lipid nanoparticles obtained in step S5 by using a sterile needle tube, stirring at constant temperature of 30 ℃ for 0.5h, and volatilizing ethanol completely to obtain the enteric coating lipid nanoparticle oral liquid.
Example 3
A preparation method of an anti-alcoholism enteric-coated lipid nanoparticle oral liquid comprises the following steps:
s1: taking fresh, rotten, mildewed and worm-eaten-free hovenia dulcis thunb, pueraria flower and passion fruit, cleaning with clear water, airing, and cutting the passion fruit into blocks and removing seeds; vacuum drying at 130pa and 55 deg.C until the water content is lower than 8%, pulverizing with pulverizer, and sieving with 100 mesh sieve to obtain semen Hoveniae powder, flos Puerariae Lobatae powder and passion fruit powder;
s2: the raw materials comprise semen hoveniae powder in percentage by mass: distilled water =1: 9, soaking the hovenia dulcis thunb powder and the distilled water obtained in the step S1 for decocting for 1.5h with slow fire, filtering to obtain filtrate, then adding the same mass of distilled water into the filter residue for decocting for 1.5h with slow fire, filtering to obtain filtrate, and combining the two filtrates to obtain the hovenia dulcis thunb extracting solution; preparing flos Puerariae Lobatae extract and Passiflora edulis extract according to the same method;
s3: uniformly mixing the hovenia dulcis thunb extract, the pueraria flower extract and the passion fruit extract in the step S2 for later use, wherein the volume ratio of the hovenia dulcis thunb extract is as follows: and (3) extracting the pueraria flower: passion fruit extract =1:2: 3;
s4: according to the mass ratio of 1:3= glyceryl monostearate: weighing glycerol monostearate and soybean lecithin, and then mixing the glycerol monostearate and the soybean lecithin in a mass ratio of 1:70= (glycerol monostearate and soybean lecithin): anhydrous ethanol, weighing the anhydrous ethanol, and uniformly stirring the mixture in a water bath at the temperature of 80 ℃; adding the mixed extracting solution prepared in the step S3, and uniformly stirring to be used as an oil phase;
s5: at a volume ratio of 1:4= oil phase in step S4: 1.5% poloxamer F68, weighing oil phase and 1.5% poloxamer F68 separately, heating 1.5% poloxamer F68 to 80 ℃ to obtain water phase; slowly injecting the oil phase into the water phase by using an injector with a No. 5 needle, keeping the temperature at 80 ℃, stirring at 1500r/min until the system is concentrated to 1/3 of the total volume of the original water phase and the oil phase, then quickly dispersing the system into sterile ice water with the volume of 3 times, stirring for 2.5h in an ice bath, and centrifuging at 5000r/min for 10min to obtain solid lipid nanoparticles;
s6: weighing medicinal polyacrylic resin to prepare 10mg/ml enteric coating, injecting the coating solution into the lipid nanoparticles in the step S5 by using a sterile needle tube, stirring at the constant temperature of 30 ℃ for 0.5h, and completely volatilizing ethanol to obtain the enteric coating lipid nanoparticle oral liquid.
Example 4
A preparation method of an anti-alcoholism enteric-coated lipid nanoparticle oral liquid comprises the following steps:
s1: taking fresh, rotten, mildewed and worm-eaten-free hovenia dulcis thunb, pueraria flower and passion fruit, cleaning with clear water, airing, and cutting the passion fruit into blocks and removing seeds; vacuum drying at 45 deg.C under 100pa until the water content is lower than 8%, pulverizing with pulverizer, and sieving with 90 mesh sieve to obtain semen Hoveniae powder, flos Puerariae Lobatae powder and passion fruit powder;
s2: the raw materials comprise semen hoveniae powder in percentage by mass: distilled water =1: 9, soaking the hovenia dulcis thunb powder and the distilled water obtained in the step S1 for decocting for 1.5h with slow fire, filtering to obtain filtrate, then adding the same mass of distilled water into the filter residue for decocting for 1.5h with slow fire, filtering to obtain filtrate, and combining the two filtrates to obtain the hovenia dulcis thunb extracting solution; preparing flos Puerariae Lobatae extract and Passiflora edulis extract according to the same method;
s3: uniformly mixing the hovenia dulcis thunb extract, the pueraria flower extract and the passion fruit extract in the step S2 for later use, wherein the volume ratio of the hovenia dulcis thunb extract is as follows: and (3) extracting the pueraria flower: passion fruit extract =1:2: 3;
s4: according to the mass ratio of 1:4= glyceryl monostearate: weighing glycerol monostearate and soybean lecithin, and then mixing the raw materials in a mass ratio of 1:80= (glycerol monostearate and soybean lecithin): anhydrous ethanol, weighing the anhydrous ethanol, and uniformly stirring the mixture in a water bath at the temperature of 80 ℃; adding the mixed extracting solution prepared in the step S3, and uniformly stirring to be used as an oil phase;
s5: at a volume ratio of 1:4= oil phase in step S4: 1.5% poloxamer F68, weighing oil phase and 1.5% poloxamer F68 separately, heating 1.5% poloxamer F68 to 80 ℃ to obtain water phase; slowly injecting the oil phase into the water phase by using an injector with a No. 5 needle, keeping the temperature at 80 ℃, stirring at 1500r/min until the system is concentrated to 1/3 of the total volume of the original water phase and the oil phase, then quickly dispersing the system into sterile ice water with the volume of 3 times, stirring for 2.5h in an ice bath, and centrifuging at 5000r/min for 15min to obtain solid lipid nanoparticles;
s6: weighing medicinal polyacrylic resin to prepare 20mg/ml enteric coating, injecting the coating solution into the lipid nanoparticles obtained in step S5 by using a sterile needle tube, stirring at constant temperature of 35 ℃ for 0.5h, and volatilizing ethanol completely to obtain the enteric coating lipid nanoparticle oral liquid.
And (3) determining the hangover alleviating effect:
30 volunteers were collected, and were guaranteed to drink 60ml of 40% VOL vodka in a non-fasting state, and then drink 20ml of anti-hangover enteric-coated lipid nanoparticle oral liquid (blank and clear water as controls), and the alcohol content in the volunteers was detected using a blown alcohol detector. The alcohol detector value is 0, and the time of drinking the anti-alcoholic enteric coating lipid nanoparticle oral liquid is shortened by 80-95min compared with a blank group and 60-76min compared with a clear water group.
Lipid nanoparticle stability testing:
the prepared enteric-coated lipid nanoparticle oral liquid is treated by artificial gastric juice for 2 hours, the effect seen by naked eyes after the artificial intestinal juice is treated for 45 minutes is better than that of a blank group, a large amount of flocculent precipitates are generated after the gastric acid treatment, the coagulation effect is good, the dispersion effect is good after the artificial intestinal juice is treated for 45 minutes, a coating film can be considered to be formed on the surface of the nanoparticle by the coating, and the coating film is favorable for being dispersed and absorbed and transported by intestinal tracts after oral administration.
The anti-alcoholism enteric-coated lipid nanoparticle oral liquid prepared by the invention can enable the nanoparticle medicine to completely pass through the stomach until the nanoparticle medicine is smoothly dissolved in the intestine, has ideal anti-alcoholism effect, natural materials, simple process, convenient preparation, no stimulation to human body and quick drug effect, and is an ideal anti-alcoholism medicine.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (7)
1. A preparation method of an anti-alcoholism enteric-coated lipid nanoparticle oral liquid is characterized by comprising the following steps: comprises the following steps:
s1: taking fresh, rotten, mildewed and worm-eaten-free hovenia dulcis thunb, pueraria flower and passion fruit, cleaning with clear water, airing, and cutting the passion fruit into blocks and removing seeds; vacuum drying until the water content is lower than 8%, pulverizing with pulverizer, and sieving with 80-100 mesh sieve to obtain semen Hoveniae powder, flos Puerariae Lobatae powder and passion fruit powder;
s2: taking the semen hoveniae powder and the distilled water obtained in the step S1, soaking, decocting with slow fire for 1-1.5h, filtering to obtain filtrate, then adding the same mass of distilled water into the filter residue, decocting with slow fire for 1-1.5h, filtering to obtain filtrate, and combining the two filtrates to obtain semen hoveniae extract; preparing flos Puerariae Lobatae extract and Passiflora edulis extract according to the same method;
s3: uniformly mixing the hovenia dulcis thunb extract, the pueraria flower extract and the passion fruit extract in the step S2 for later use;
s4: weighing glyceryl monostearate, soybean lecithin and absolute ethyl alcohol, and stirring uniformly in a water bath; adding the mixed extracting solution prepared in the step S3, and uniformly stirring to be used as an oil phase;
s5: heating 1.5% poloxamer F68 to 75-80 ℃ as the aqueous phase; injecting the oil phase in the step S4 into the water phase, stirring, concentrating, rapidly dispersing the system into sterile ice water, stirring in an ice bath, and centrifuging to obtain solid lipid nanoparticles;
s6: weighing medicinal polyacrylic resin to prepare 10mg/ml-20mg/ml enteric coating, injecting the coating solution into the lipid nanoparticles in the step S5 by using a sterile needle tube, stirring at constant temperature of 30-35 ℃ for 0.5h, and completely volatilizing ethanol to obtain the enteric coating lipid nanoparticle oral liquid.
2. The preparation method of the anti-hangover enteric-coated lipid nanoparticle oral liquid according to claim 1, wherein the preparation method comprises the following steps: in step S1, the vacuum drying conditions are as follows: 100-.
3. The preparation method of the anti-hangover enteric-coated lipid nanoparticle oral liquid according to claim 1, wherein the preparation method comprises the following steps: in the step S2, the weight ratio of hovenia dulcis thunb powder is: distilled water, arrowroot flour: water, passion fruit powder: the water is 1:8-1: 9.
4. The preparation method of the anti-hangover enteric-coated lipid nanoparticle oral liquid according to claim 1, wherein the preparation method comprises the following steps: in the step S3, the volume ratio of 1:1:1-1:2:3= hovenia dulcis thunb extract: and (3) extracting the pueraria flower: passion fruit extract.
5. The preparation method of the anti-hangover enteric-coated lipid nanoparticle oral liquid according to claim 1, wherein the preparation method comprises the following steps: in the step S4, the mass ratio of 1:2-1:4= glyceryl monostearate: soybean lecithin in a mass ratio of 1:60-1:80= (glyceryl monostearate and soybean lecithin): anhydrous ethanol.
6. The preparation method of the anti-hangover enteric-coated lipid nanoparticle oral liquid according to claim 1, wherein the preparation method comprises the following steps: in step S5, the volume ratio is 1:3-1:4= oil phase: 1.5% poloxamer F68.
7. The preparation method of the anti-hangover enteric-coated lipid nanoparticle oral liquid according to claim 1, wherein the preparation method comprises the following steps: in the step S5, the oil phase is injected into the water phase, the temperature is kept at 75-80 ℃, the stirring is carried out at 1000r/min-1500r/min until the system is concentrated to 1/4-1/3 of the total volume of the original water phase and oil phase, then the system is rapidly dispersed into sterile ice water with the volume of 2-3 times of the original volume, the stirring is carried out in an ice bath for 2h-2.5h, and the centrifugation is carried out at 4000r/min-5000r/min for 10min-15min, so as to prepare the solid lipid nanoparticles.
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| CN103783605A (en) * | 2013-12-20 | 2014-05-14 | 芜湖金鹰机械科技开发有限公司 | Passion fruit healthcare beverage and preparation method thereof |
| CN105456200A (en) * | 2015-12-08 | 2016-04-06 | 郑州大学 | Preparation method and application of nanoparticle microsphere for improving oral bioavailability of poorly soluble drugs |
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| CN103783605A (en) * | 2013-12-20 | 2014-05-14 | 芜湖金鹰机械科技开发有限公司 | Passion fruit healthcare beverage and preparation method thereof |
| CN105456200A (en) * | 2015-12-08 | 2016-04-06 | 郑州大学 | Preparation method and application of nanoparticle microsphere for improving oral bioavailability of poorly soluble drugs |
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