CN111248160A

CN111248160A - Fly maggot oviposition inducer and application thereof

Info

Publication number: CN111248160A
Application number: CN202010201561.3A
Authority: CN
Inventors: 曹帆; 刘胜利; 安振华
Original assignee: Yizheng Zhengtaiyong Biotechnology Co Ltd
Current assignee: Yizheng Zhengtaiyong Biotechnology Co Ltd
Priority date: 2020-03-20
Filing date: 2020-03-20
Publication date: 2020-06-09

Abstract

The invention belongs to the technical field of fly maggot breeding, and discloses a fly maggot oviposition inducer which comprises a base material and a humectant, wherein the mass part ratio of the base material to the humectant is (1-1.5): 1, wherein the base material comprises milk powder, brown sugar, wheat bran and water, and the humectant comprises sawdust and ammonia water. Meanwhile, the invention also provides a method for inducing the fly maggots to lay eggs, which comprises the following steps of fully and uniformly stirring the base material of the oviposition inducer, then placing the base material on the bottom layer of the fly maggot seed receiving box, fully and uniformly stirring the humectant, then placing the base material on the base material, taking out the base material after 24 hours, simultaneously placing the base material into a new seed receiving box, and circularly collecting the fly maggot eggs in such a way. The housefly maggot oviposition inducer can induce houseflies to produce seeds for 18-24 hours above the housefly maggot oviposition inducer, and the phenomenon of producing seeds at other places can not occur in the first 12 hours, so that the phenomenon of stacking and death can be greatly avoided.

Description

Fly maggot oviposition inducer and application thereof

Technical Field

The invention belongs to the technical field of fly maggot breeding, and particularly relates to a fly maggot spawning inducer and application thereof.

Background

Fly maggots, which are larvae of flies. Mainly in human and animal manure piles, garbage and rotten substances, and some of the manure and rotten substances live in rotten animal bodies. Pupating under the soil surface, and overwintering with pupae, wherein the depth of overwintering pupae in the soil can reach about 10 cm.

At present, fly maggots can be used as a high-efficiency protein feed for insects, the demand on aquatic products is very vigorous, but the breeding of the fly maggots is in the starting stage, and many traditional technologies are low in application efficiency and are immature and inconvenient. The breeding of the fly larvae is generally high-density breeding, wherein an important link is the induction of the egg laying of the fly larvae, so that a large amount of fly larvae are obtained. According to the records of the prior art, the fly maggot breeding generally adopts a substrate, feed and a substance for increasing the egg collecting effect as an egg collecting substance, wherein the egg collecting substance can be animal meat, animal blood clots and fly maggot residues, but the source quality and sanitary condition of slaughtering wastes such as the blood clots and the like cannot be strictly guaranteed, and the fly maggot breeding efficiency is greatly influenced; meanwhile, wheat bran, ammonia water or poultry feed is also adopted as an egg collection inducer in the prior art, but the induction aging is far insufficient to support the requirement on housefly larvae in actual production. Meanwhile, the attraction of the traditional egg collecting inducer to the houseflies is insufficient, so that the housefly larvae easily produce seeds everywhere, the breeding environment is damaged, meanwhile, the economic benefit is reduced due to the wasted eggs, and the traditional egg collecting mode is easy to stack and kill under the high-density breeding. Therefore, a method capable of efficiently inducing the fly maggots to lay eggs and efficiently culturing the fly maggots in a high-density culture mode is urgently needed.

Disclosure of Invention

In order to solve the technical problems, the invention provides a fly maggot spawning inducer and a method for inducing the fly maggots to spawn by adopting the inducer. By adopting the fly maggot spawning induction method, the spawning inducer is placed in a double layer manner, and the fly maggots are induced to spawn in the high-density culture mode, so that the phenomenon of seed disorderly production can be effectively reduced, the stacked death generated in the high-density culture mode is avoided, the spawning induction rate can be greatly improved, and the spawning is efficiently collected.

In a first aspect, a certain embodiment of the present invention provides a fly maggot oviposition inducer, which comprises a base material and a humectant, wherein the base material is composed of milk powder, brown sugar, wheat bran and water.

The fly maggot oviposition inducer has the beneficial effects that: the base material components completely adopt natural non-industrial chemical raw materials, the source is safe and non-toxic, a fluffy egg laying environment can be provided for the egg laying of the fly maggots, and meanwhile, the humectant in the inducer can effectively ensure the required environmental humidity of the fly maggots in the egg laying process, so that the egg laying efficiency of the fly maggots is improved.

On the basis of the technical scheme, the invention can be further improved as follows:

in some embodiments, the mass part ratio of the base material to the humectant is (1-1.5): 1.

the technical scheme of the certain embodiments has the beneficial effects that: the mass part ratio of the base material to the humectant can be controlled, the seed receiving time of a single seed receiving box can be greatly prolonged, and the effect can be generated for 24 hours.

In some embodiments, the ratio of the milk powder to the brown sugar to the wheat bran to the water in parts by weight is (0.5-1.5): (1-3): (50-150): (50-100).

The technical scheme of the certain embodiments has the beneficial effects that: wheat bran is used as a main component, and a small amount of milk powder and brown sugar are added, so that the nutrition can be greatly increased, the larvae are induced to feed to the base material, and the situation that the larvae incubated in advance feed to the place where the humectant is removed and the imagoes are interfered to lay eggs is avoided.

In certain embodiments, the ratio of the milk powder to the brown sugar to the wheat bran to the water is 1:2:100:75 by weight.

In certain embodiments, the humectant consists of sawdust and a substance capable of emitting an ammonia odor.

In some embodiments, the mass ratio of the sawdust to the substance capable of emitting ammonia odor is (0.5-1.5): (1.5-2.5).

In certain embodiments, the substance capable of emitting an ammonia odor is preferably ammonia.

In certain embodiments, the mass part ratio of the sawdust to the ammonia water is 1: 2.

The technical scheme of the certain embodiments has the beneficial effects that: the ammonia water is the inducer of laying eggs, and the source is convenient and can save the cost, adopts no nutritive value's sawdust and ammonia water stirring, forms fluffy humectant, can produce strong attraction to the adult simultaneously, reduces the wheat bran use amount by a wide margin, and the loose degree of sawdust is higher, is favorable to collecting more worm's eggs.

In a second aspect, the present invention provides a method for inducing maggots to lay eggs by using the said inducing agent, comprising the following steps:

a step of mixing base materials, namely stirring and mixing the milk powder, the brown sugar, the wheat bran and the water uniformly to obtain the mixed base materials,

a step of mixing the humectant, namely stirring and uniformly mixing the sawdust and ammonia water to obtain the mixed humectant,

a step of preparing a seed receiving box, namely placing the mixed base material at the bottom of the seed receiving box, and covering the mixed humectant on the mixed base material to obtain the prepared seed receiving box;

in some embodiments, the base material is placed at the bottom of the seed receiving box and is laid or stacked at the bottom of the seed receiving box, the thickness of the laid base material is 1.5-2.5cm, and the thickness of the inducer is not more than 4.5 cm.

And a step of inducing spawning, wherein the prepared seed receiving box is put into the culture bin when more than 4 days after the maggots pupae in the culture bin emerge into imagoes, and the imagoes are taken out after 18 to 24 hours.

In certain embodiments, the humidity in the cultivation silo is maintained at 60-75%, the humidity in the cultivation silo is 60-75%, preferably 60%, and the temperature in the cultivation farm is 26-28 ℃, preferably 28 ℃.

In some embodiments, the method further comprises the step of simultaneously placing the newly configured seed receiving boxes, and the steps are repeated.

Compared with the prior art, the invention has the beneficial effects that:

1) the spawning inducer of the invention adopts safe and non-toxic natural ingredients as the maggot spawning inducer, which can effectively avoid the toxicity to the maggot spawning, and has convenient acquisition of ingredient sources and cost saving.

2) The spawning inducer disclosed by the invention is combined with the humectant by adopting the base material, so that the humidity of the inducer and the humidity in a spawning environment can be kept simultaneously on the basis of providing nutrition, the continuous spawning is kept for 18-24 hours, and the spawning time is effectively prolonged.

3) According to the induced spawning method, the inducers are placed in double layers, the lower layer takes wheat bran as a main component and is matched with a small amount of milk powder and brown sugar, nutrition can be greatly increased, larvae are induced to be fed to a base material, and the upper layer is a fluffy sawdust and ammonia water mixture and can induce adults to spawn.

4) By adopting the method for inducing the fly maggots to lay eggs, the laid-egg receiving boxes can be taken out and placed into new receiving boxes in a high-density culture mode, so that the eggs can be collected for 20 days continuously in a circulating manner, and the eggs are efficiently collected.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments will be briefly described below.

FIG. 1 statistical analysis of induced larva weight in example 3

FIG. 2 statistical analysis of induced larva weight in example 4

FIG. 3 in example 4, the stacked death pattern induced by oviposition by the inducer of the invention and the conventional inducer, wherein FIG. A is the stacked death pattern during induction by the inducer of the invention, and FIGS. B-D are the stacked death patterns during induction by the conventional inducers 1-3

Detailed Description

Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby. It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains.

Example 1

Respectively taking milk powder, brown sugar, wheat bran and water in certain parts by mass, mixing and stirring uniformly to obtain a mixed base material, simultaneously taking sawdust and ammonia water in certain parts by mass, mixing and stirring uniformly to obtain a mixed humectant, and preparing the two into the fly maggot oviposition inducer 1-8, wherein the specific ratio of each component is shown in table 1.

TABLE 1 spawning inducers with different mass portion ratios

Example 2

The flyblow was induced to lay eggs using the inducers 1 to 8 in table 1 of example 1. Firstly, placing the mixed base material at the bottom of a seed receiving box (26cm multiplied by 19cm multiplied by 7cm), then covering the mixed humectant on the base material, and sequentially preparing the seed receiving boxes 1-8 for inducing the maggots to lay eggs. After the fly maggots in the breeding bins emerge into adults, generally for 4-10 days, the prepared seed receiving boxes 1-8 are sequentially and respectively placed in different breeding bins (breeding bins 1-8) of the same breeding room. After 24 hours of induced spawning, the seed receiving box is taken out, the collected worm eggs are cultivated to the terminal stage of 3-year-old larvae, and the larvae are separated and weighed (jin). The same operation was repeated three times, and the specific weights are shown in Table 2.

Table 2 induced larva weighing statistical table (jin)

Breeding bin	No. 1 storehouse	No. 2 storehouse	No. 3 storehouse	No. 4 storehouse	No. 5 storehouse	No. 6 storehouse	No. 7 storehouse	No. 8 storehouse
									Day one	11.6	12.1	12.3	11.9	9.4	7.8	7.3	5.9
The next day	12.4	11.9	11.9	11.9	9.2	8.2	7.2	6.1
									The third day	12.3	11.7	12.6	12.2	8.9	8.4	6.9	6.2
Mean value of	12.1	11.9	12.3	12.0	9.2	8.1	7.1	6.1

As can be seen from the weight of the larvae in Table 2, the inducers in the No. 1 compartment (inducer 1) to the No. 4 compartment (inducer 4) can effectively induce the fly maggots to lay eggs, and the weight of the larvae can reach 12.1 jin on average. When the weight of No. 5 cabin larvae without milk powder in the inducer is 9.2 jin, the weight of No. 6 cabin without brown sugar is only 8.1 jin, the weight of No. 7 cabin larvae without wheat bran is only 7.1 jin, and the weight of No. 8 cabin larvae without sawdust is only 6.1 jin, the weight is reduced by 50% compared with the average value of No. 1-4 cabins of 12.1. Therefore, the fly maggot oviposition inducer with the specific components can well induce the fly maggots to oviposit.

Example 3

The inducer 4 of example 1 was prepared into a spawning box according to the method of example 2, and meanwhile, a conventional fly maggot spawning inducer was mixed and spread at the bottom of the spawning box as a control, and the conventional fly maggot spawning inducer had the following components: wheat bran and ammonia water in a weight ratio of 1:2, and a traditional inducer of 2: wheat bran, ammonia water and chicken blood in a weight ratio of 1:1:0.5, and a traditional inducer 3: the weight part ratio of the bran to the bean dregs to the milk powder is 1:0.5: 0.5. The fly maggot oviposition inducer of the invention is respectively combined with the traditional fly maggot oviposition inducer (traditional inducer 1-3) into a group (2 spawning boxes) and put into the same breeding chamber in the same breeding room. The spawning box is taken out after 24 hours, the collected worm eggs are cultivated to the terminal stage of 3-year-old larvae, and the larvae are separated and weighed (jin). The same operation was repeated three times, and the specific weights are shown in Table 3.

TABLE 3 induced larva weighing statistical table

Through statistical variance analysis (see figure 1), the weight of the larvae induced by the inducer is very obvious (P is less than 0.01) difference with the weight of the larvae induced by the traditional spawning inducer in the prior art in the same breeding bin, in the No. 1 bin, the weight of the larvae induced by the traditional inducer 1 is only 8.3 jin, while the weight of the larvae induced by the inducer is 12.1 jin, which is increased by 45.8%; in the No. 2 cocklebur, the weight of the larva induced by the traditional inducer 2 is only 7.8 jin, while the weight of the larva induced by the inducer provided by the invention is 12.3 jin, and the weight is increased by 57.7%; in the No. 3 storehouse, the weight of the larva induced by the traditional inducer 3 is only 7.5 jin, while the weight of the larva induced by the inducer provided by the invention is 12.1 jin, and the weight is increased by 61.3%. As can be seen, the attraction of the inducer of the invention to the fly maggots is obviously stronger than that of the traditional inducer, and the fly maggots can be continuously induced to lay eggs within 24 hours.

Example 4

The inducer 4 of example 1 was prepared into a spawning box according to the method of example 2, and meanwhile, a conventional fly maggot spawning inducer was mixed and spread at the bottom of the spawning box as a control, and the conventional fly maggot spawning inducer had the following components: wheat bran and ammonia water in a weight ratio of 1:2, and a traditional inducer of 2: wheat bran, ammonia water and chicken blood in a weight ratio of 1:1:0.5, and a traditional inducer 3: the weight part ratio of the bran to the bean dregs to the milk powder is 1:0.5: 0.5. The spawning inducer spawning box for the maggots and the spawning inducer for the traditional maggots (the traditional inducer 1-3) are respectively placed in different breeding bins (breeding bins 1-4) in the same breeding room, and 2 spawning boxes are repeatedly placed in each breeding bin. The spawning box is taken out after 24 hours, the collected worm eggs are cultivated to the terminal stage of 3-year-old larvae, and the larvae are separated and weighed (jin). The same operation was repeated four times, and the specific weights are shown in Table 4.

TABLE 4 induced larva weighing statistical table

Through statistical analysis of variance (see fig. 2), it can be seen that the weight of larvae induced by the inducer is 13.1 jin on average, which is very significant (P <0.01) different from the weight of larvae induced by the conventional oviposition inducer in the prior art, and is significantly higher than that of the conventional fly maggot inducer in the prior art, and meanwhile, the oviposition inducer of the present invention does not have stacked death phenomenon in the process of inducing the adult to oviposition (see fig. 3A), while the conventional oviposition inducer has a large amount of stacked death phenomenon in the process of inducing the adult to oviposition (see fig. 3B-fig. 3D). Therefore, the induction effect of the inducer is far better than that of the traditional inducer, the survival rate of adults is effectively ensured, and more worm eggs can be continuously collected.

Unless specifically stated otherwise, the numerical values set forth in these examples do not limit the scope of the invention. In all examples shown and described herein, unless otherwise specified, any particular value should be construed as merely illustrative, and not restrictive, and thus other examples of example embodiments may have different values.

Claims

1. The fly maggot oviposition inducer is characterized by comprising a base material and a humectant, wherein the base material comprises milk powder, brown sugar, wheat bran and water.

2. The inducer according to claim 1, wherein the ratio of the base material to the humectant is (1-1.5): 1.

3. the inducer according to claim 1, wherein the ratio of the milk powder, the brown sugar, the wheat bran and the water in parts by mass is (0.5-1.5): (1-3): (50-150): (50-100).

4. The inducer of claim 3, wherein the ratio of the milk powder, the brown sugar, the wheat bran and the water is 1:2:100:75 by weight.

5. The induction agent according to claim 1, wherein said humectant consists of sawdust and a substance capable of emitting ammonia odor.

6. The inducer according to claim 5, wherein the ratio of the sawdust and the substance capable of emitting ammonia smell is (0.5-1.5): (1.5-2.5), preferably 1: 2.

7. The induction agent according to claim 6, wherein the substance capable of emitting ammonia odor is ammonia water.

8. A method for inducing maggots to lay eggs by using the inducing agent of claim 7, comprising the steps of:

a step of mixing base materials, namely stirring and mixing the milk powder, the brown sugar, the wheat bran and the water uniformly to obtain the mixed base materials;

a step of mixing the humectant, namely stirring and uniformly mixing the sawdust and ammonia water to obtain a mixed humectant;

and a step of inducing spawning, wherein after the fly maggots pupae in the culture bin emerge into adults, the prepared seed receiving box is placed into the culture bin and is taken out after 18-24 hours.

9. The method according to claim 8, wherein the humidity in the culture bin is 60-75%, preferably 60 ℃, the temperature in the farm is 26-28 ℃, preferably 28 ℃, the base material is placed at the bottom of the seed receiving box by being laid flat or stacked at the bottom of the seed receiving box, the thickness of the laid flat is 1.5-2.5cm, and the thickness of the inducer is not more than 4.5 cm.

10. The method of claim 9, further comprising simultaneously placing a newly configured seed receiving cassette and repeating.