CN111228422A - Traditional Chinese medicine composition for reducing intramuscular fat of chicken and application and preparation method thereof - Google Patents
Traditional Chinese medicine composition for reducing intramuscular fat of chicken and application and preparation method thereof Download PDFInfo
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- CN111228422A CN111228422A CN202010248271.4A CN202010248271A CN111228422A CN 111228422 A CN111228422 A CN 111228422A CN 202010248271 A CN202010248271 A CN 202010248271A CN 111228422 A CN111228422 A CN 111228422A
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a traditional Chinese medicine composition for reducing intramuscular fat of chicken and a use and preparation method thereof; belongs to the technical field of traditional Chinese medicine cultivation; the traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: 4-12 parts of bighead atractylodes rhizome, 4-12 parts of poria cocos, 4-12 parts of coix seed, 2-8 parts of rhizoma atractylodis, 0.5-6 parts of rheum officinale, 0.5-6 parts of rhizoma alismatis, 0.5-6 parts of dried orange peel, 0.5-6 parts of fructus aurantii, 0.5-6 parts of eupatorium and 0.5-6 parts of rhizoma cyperi; the invention aims to provide a traditional Chinese medicine composition for reducing intramuscular fat of chicken with scientific compatibility and good effect, and a use and preparation method thereof; is used for broiler chicken breeding.
Description
Technical Field
The invention relates to a traditional Chinese medicine composition for chicken, and particularly relates to a traditional Chinese medicine composition for reducing intramuscular fat of chicken. The invention also relates to a preparation method and a use method of the traditional Chinese medicine composition.
Background
Lipids are a generic term for fats and lipids. It not only supplies energy required by the body, but also provides essential fatty acid required by the body. However, the rapid growth of chickens often causes excessive deposition of body fat, especially abdominal fat and subcutaneous fat, reduces feed conversion rate and carcass quality, and is also prone to induce laying hen fatty liver syndrome and endangers the healthy growth of chickens. Therefore, how to reduce the excessive accumulation of chicken abdominal fat and subcutaneous fat becomes a problem which is concerned by production managers and scientific researchers together.
Disclosure of Invention
The first purpose of the present invention is to provide a traditional Chinese medicine composition for reducing intramuscular fat of chicken with scientific compatibility and good effect, which overcomes the defects of the prior art.
The second objective of the invention is to provide a method for preparing the decoction of the traditional Chinese medicine composition.
The third objective of the invention is to provide a using method of the traditional Chinese medicine composition decoction.
The first technical scheme of the invention is realized as follows: a traditional Chinese medicine composition for reducing intramuscular fat of chicken is prepared from the following traditional Chinese medicine raw materials in parts by weight: 4-12 parts of bighead atractylodes rhizome, 4-12 parts of poria cocos, 4-12 parts of coix seed, 2-8 parts of rhizoma atractylodis, 0.5-6 parts of rheum officinale, 0.5-6 parts of rhizoma alismatis, 0.5-6 parts of dried orange peel, 0.5-6 parts of fructus aurantii, 0.5-6 parts of eupatorium and 0.5-6 parts of rhizoma cyperi.
The traditional Chinese medicine composition for reducing intramuscular fat of chicken is prepared from the following traditional Chinese medicine raw materials in parts by weight: 5-9 parts of bighead atractylodes rhizome, 5-9 parts of poria cocos, 5-9 parts of coix seed, 3-6 parts of rhizoma atractylodis, 1-3 parts of rheum officinale, 1-3 parts of rhizoma alismatis, 1-5 parts of dried orange peel, 1-5 parts of fructus aurantii, 1-5 parts of eupatorium and 1-5 parts of rhizoma cyperi.
The traditional Chinese medicine composition for reducing intramuscular fat of chicken is prepared from the following traditional Chinese medicine raw materials in parts by weight: 7 parts of bighead atractylodes rhizome, 7 parts of poria cocos, 8 parts of coix seed, 4 parts of rhizoma atractylodis, 2 parts of rheum officinale, 2 parts of rhizoma alismatis, 3 parts of dried orange peel, 3 parts of fructus aurantii, 3 parts of eupatorium and 3 parts of rhizoma cyperi.
The second technical scheme of the invention is realized as follows: a method for preparing the traditional Chinese medicine composition comprises the following steps:
(1) weighing the traditional Chinese medicine raw materials according to the parts by weight, smashing the raw materials, uniformly mixing the raw materials to form traditional Chinese medicine mixed powder, and weighing the traditional Chinese medicine mixed powder according to the dosage of 0.8-2 g of receptor per kilogram of body weight for later use; (2) putting the weighed mixed powder of the traditional Chinese medicines into a ceramic container, soaking and decocting into decoction.
In the preparation method of the traditional Chinese medicine composition, the step (2) is specifically as follows: putting the weighed mixed powder of the traditional Chinese medicines into a ceramic container, adding clear water with the volume of 3-5 times of the volume of the mixed powder of the traditional Chinese medicines according to the volume of the mixed powder of the traditional Chinese medicines, soaking for 30 minutes, boiling with strong fire, then slowly decocting for 10-15 minutes with slow fire, and filtering to obtain primary medicine juice; rehydrating 1-2 times the original Chinese medicinal powder by volume, decocting with strong fire for 15-20 min, standing, naturally cooling to room temperature, filtering to obtain secondary medicinal liquid, and mixing the primary medicinal liquid and the secondary medicinal liquid to obtain Chinese medicinal decoction.
The third technical scheme of the invention is realized as follows: a use method of the traditional Chinese medicine composition decoction comprises the following steps: the water supply of the chickens is stopped for two hours before the administration, then the total amount of water required by the chickens to be fed with the medicine is calculated according to the drinking amount of each chicken for two hours, and the decoction is mixed with water according to the drinking amount to obtain the liquid medicine which is taken once a day.
The medicine properties of the traditional Chinese medicine raw materials are as follows:
bai Zhu is warm in nature, sweet and bitter in taste. It enters spleen and stomach meridians.
The efficacy is mainly as follows: invigorating spleen, invigorating qi, eliminating dampness, promoting diuresis, arresting sweating, and preventing miscarriage. They belong to the category of qi tonics under deficiency-tonifying herbs.
Poria cocos, sweet and bland in flavor, mild in nature. It enters heart, lung, spleen and kidney meridians.
The efficacy is mainly as follows: induce diuresis and drain dampness, invigorate spleen and calm heart. Can be used for treating edema, oliguria, phlegm and fluid retention, dizziness, palpitation, spleen deficiency, anorexia, loose stool, diarrhea, uneasiness, palpitation, and insomnia.
Coix seed, sweet and bland, slightly cold. It enters spleen, stomach and lung meridians.
The efficacy is mainly as follows: induce diuresis and drain dampness, invigorate spleen to arrest diarrhea, dispel dampness and remove arthralgia, clear heat and expel pus. Can be used for treating dysuria, edema, loempe, diarrhea due to spleen deficiency, rheumatalgia, spasm and spasm of tendons and vessels, pulmonary abscess, and intestinal abscess.
Cang Zhu is pungent and bitter in flavor and warm in nature. It enters spleen, stomach and liver meridians.
The efficacy is mainly as follows: dry dampness and invigorate spleen, dispel wind and cold, improve vision. Can be used for treating damp obstruction of middle warmer, abdominal distention, diarrhea, edema, tinea pedis, atrophic debility cramped, rheumatalgia, wind-cold type common cold, night blindness, dim eyesight, and astringency.
Rhubarb, bitter in taste and cold in nature, enters stomach, large intestine, liver and spleen channels, has the efficacies of removing food retention, clearing damp-heat, purging fire, cooling blood, removing blood stasis and detoxifying, and is mainly used for treating excess heat constipation; heat retention in the chest and fullness; dysentery due to damp-heat; jaundice; gonorrhea; edema and abdominal fullness; difficulty in urination; conjunctival congestion; swollen and sore throat; sores in the mouth and tongue; vomiting due to stomach heat; hematemesis; hemoptysis; bleeding; hematochezia; hematuria; storing blood; amenorrhea; postpartum abdominal pain due to stasis; the accumulation of symptoms; traumatic injury; carbuncles and ulcers due to heat toxin; erysipelas; scald and the like.
Ze Xie is sweet in flavor and cold in nature. It enters kidney and bladder meridians.
The efficacy is mainly as follows: induce diuresis, drain dampness and purge heat. It can be used for treating dysuria, edema, emesis, dysentery, phlegm retention, tinea pedis, gonorrhea, and hematuria.
Tangerine peel, pericarpium Citri Reticulatae is warm in nature, pungent and bitter in flavor. It enters lung and spleen meridians.
The efficacy is mainly as follows: regulate qi-flowing for strengthening spleen, regulate middle energizer, dry dampness and resolve phlegm. Can be used for treating abdominal distention, anorexia, vomiting, diarrhea, cough, and excessive phlegm.
Fructus Aurantii is warm in nature, bitter, pungent and sour in flavor. It enters spleen and stomach meridians.
The efficacy is mainly as follows: regulating qi-flowing, relieving epigastric distention, activating stagnancy and relieving flatulence. Pertains to qi-regulating herbs and is mainly indicated for qi stagnation in chest and hypochondrium, fullness and pain, indigestion, phlegm-fluid retention, and viscera prolapse.
Eupatorium fortunei, pungent in flavor, neutral in nature, enters spleen, stomach and lung meridians.
The efficacy is mainly as follows: relieving summer-heat and resolving dampness, removing filth and regulating the middle warmer. It can be used for the treatment of summer-heat dampness, headache due to cold and heat, damp accumulation in the interior, gastric distention and fullness without hunger, nausea, emesis, sweet and greasy taste, and diabetes.
Cyperus rotundus is pungent, slightly bitter, slightly sweet and mild in taste. It enters liver, spleen and triple energizer meridians.
The efficacy is mainly as follows: to soothe the liver, relieve depression, regulate qi, relieve epigastric distention, regulate menstruation and alleviate pain. Can be used for treating stagnation of qi due to depression of the liver, distending pain in chest and hypochondrium, hernia pain, breast pain, stagnation of qi in spleen and stomach, abdominal distention, fullness and pain, menoxenia, amenorrhea, and dysmenorrhea.
After the formula is adopted, the bighead atractylodes rhizome, the poria cocos and the coix seed are used as main medicines in the formula, the spleen is strengthened, the dampness is removed, the rhizoma atractylodis is supplemented, the dampness is removed, the spleen is enlivened, the rhubarb and the rhizoma alismatis are used as auxiliary medicines, the purgation and the accumulation elimination are performed, the water channel is regulated, the dried orange peel, the bitter orange, the eupatorium and the rhizoma cyperi are used for dissipating dampness with aromatics, and the qi regulating and the middle warmer are used as adjuvant medicines, so that.
In addition, the formula uses rhubarb and rhizoma alismatis for purgation and accumulation elimination according to the physiological characteristics that the intestinal tract of the chicken is short, the muscle stomach is covered by the endothelium corneum gigeriae galli and the like, and the drug effect is enhanced; guiding the drugs with dried orange peel, bitter orange, fortune eupatorium herb and nutgrass galingale rhizome to the channels to promote the water liquid to be gasified, and then discharging urine out of the body.
The formula is prepared into decoction, and the mode of feeding the traditional Chinese medicine is adopted when water is cut off, so that each chicken can take the traditional Chinese medicine in a sufficient amount, and the effect of reducing the blood fat of the chicken is ensured.
Drawings
The invention will be further described in detail with reference to examples of embodiments shown in the drawings to which, however, the invention is not restricted.
FIG. 1 is an electrophoretogram of extracted RNA in the experimental example of the present invention;
FIG. 2 is a standard curve and a melting curve of β -actin gene, FAS gene and LPL gene in the experimental examples of the present invention.
Wherein A, C, E are standard curves of β -actin gene, FAS gene and LPL gene respectively, and B, D, F are dissolution curves of β -actin gene, FAS gene and LPL gene respectively.
Detailed Description
The present invention will be described in further detail with reference to the following examples, but the present invention is not limited thereto.
The invention relates to a traditional Chinese medicine composition for reducing intramuscular fat of chicken, which is characterized by being prepared from the following traditional Chinese medicine raw materials in parts by weight: 4-12 parts of bighead atractylodes rhizome, 4-12 parts of poria cocos, 4-12 parts of coix seed, 2-8 parts of rhizoma atractylodis, 0.5-6 parts of rheum officinale, 0.5-6 parts of rhizoma alismatis, 0.5-6 parts of dried orange peel, 0.5-6 parts of fructus aurantii, 0.5-6 parts of eupatorium and 0.5-6 parts of rhizoma cyperi.
Preferably, the traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: 5-9 parts of bighead atractylodes rhizome, 5-9 parts of poria cocos, 5-9 parts of coix seed, 3-6 parts of rhizoma atractylodis, 1-3 parts of rheum officinale, 1-3 parts of rhizoma alismatis, 1-5 parts of dried orange peel, 1-5 parts of fructus aurantii, 1-5 parts of eupatorium and 1-5 parts of rhizoma cyperi.
Further preferably, the traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: 7 parts of bighead atractylodes rhizome, 7 parts of poria cocos, 8 parts of coix seed, 4 parts of rhizoma atractylodis, 2 parts of rheum officinale, 2 parts of rhizoma alismatis, 3 parts of dried orange peel, 3 parts of fructus aurantii, 3 parts of eupatorium and 3 parts of rhizoma cyperi.
The invention relates to a method for preparing the traditional Chinese medicine composition decoction, which comprises the following steps:
(1) weighing the traditional Chinese medicine raw materials according to the parts by weight, smashing the raw materials, uniformly mixing the raw materials to form traditional Chinese medicine mixed powder, and weighing the traditional Chinese medicine mixed powder according to the dosage of 0.8-2 g of receptor per kilogram of body weight for later use; (2) putting the weighed mixed powder of the traditional Chinese medicines into a ceramic container, adding clear water with the volume of 3-5 times of the volume of the mixed powder of the traditional Chinese medicines according to the volume of the mixed powder of the traditional Chinese medicines, soaking for 30 minutes, boiling with strong fire, then slowly decocting for 10-15 minutes with slow fire, and filtering to obtain primary medicine juice; rehydrating 1-2 times the original Chinese medicinal powder by volume, decocting with strong fire for 15-20 min, standing, naturally cooling to room temperature, filtering to obtain secondary medicinal liquid, and mixing the primary medicinal liquid and the secondary medicinal liquid to obtain Chinese medicinal decoction.
The invention relates to a using method of the traditional Chinese medicine composition decoction, which comprises the following steps: the water supply of the chickens is stopped for two hours before the administration, then the total amount of water required by the chickens to be fed with the medicine is calculated according to the drinking amount of each chicken for two hours, and the decoction is mixed with water according to the drinking amount to obtain the liquid medicine which is taken once a day.
Example 1
The formula is as follows: 4 parts of largehead atractylodes rhizome, 4 parts of Indian buead, 4 parts of coix seed, 2 parts of swordlike atractylodes rhizome, 0.5 part of rhubarb, 0.5 part of oriental waterplantain rhizome, 0.5 part of tangerine peel, 0.5 part of bitter orange, 0.5 part of fortune eupatorium herb and 0.5 part of nutgrass galingale rhizome.
The preparation method of the decoction comprises the following steps: (1) weighing the traditional Chinese medicine raw materials according to the parts by weight, smashing the raw materials, uniformly mixing the raw materials to form traditional Chinese medicine mixed powder, and weighing the traditional Chinese medicine mixed powder according to the dosage of 0.8 g of receptor per kilogram of body weight for later use; (2) putting the weighed mixed powder of the traditional Chinese medicines into a ceramic container, adding clear water with the volume 3 times of the volume of the mixed powder of the traditional Chinese medicines according to the volume of the mixed powder of the traditional Chinese medicines, soaking for 30 minutes, boiling with strong fire, then slowly decocting with slow fire for 105 minutes, and filtering to obtain primary medicinal juice; rehydrating 1 times the volume of the original Chinese medicinal powder, boiling with strong fire, decocting with slow fire for 15 min, standing, naturally cooling to room temperature, filtering to obtain secondary decoction, and mixing the primary decoction and the secondary decoction to obtain the final decoction.
The application method of the decoction comprises the following steps: the water supply of the chickens is stopped for two hours before the administration, then the total amount of water required by the chickens to be fed with the medicine is calculated according to the drinking amount of each chicken for two hours, and the decoction is mixed with water according to the drinking amount to obtain the liquid medicine which is taken once a day.
Example 2
The formula is as follows: 5 parts of bighead atractylodes rhizome, 5 parts of poria cocos, 5 parts of coix seed, 3 parts of rhizoma atractylodis, 1 part of rheum officinale, 1 part of rhizoma alismatis, 1 part of dried orange peel, 1 part of fructus aurantii, 1 part of herba eupatorii and 1 part of rhizoma cyperi.
The preparation method of the decoction comprises the following steps: (1) weighing the traditional Chinese medicine raw materials according to the parts by weight, smashing the raw materials, uniformly mixing the raw materials to form traditional Chinese medicine mixed powder, and weighing the traditional Chinese medicine mixed powder according to the dosage of 1.5 g of receptor per kilogram of body weight for later use; (2) putting the weighed mixed powder of the traditional Chinese medicines into a ceramic container, adding clear water with the volume 4 times of the volume of the mixed powder of the traditional Chinese medicines according to the volume of the mixed powder of the traditional Chinese medicines, soaking for 30 minutes, boiling with strong fire, then slowly decocting with slow fire for 12 minutes, and filtering to obtain primary medicinal juice; rehydrating 1.5 times the original Chinese medicinal powder by volume, decocting with strong fire for 18 min, standing, naturally cooling to room temperature, filtering to obtain secondary medicinal liquid, and mixing the primary medicinal liquid and the secondary medicinal liquid to obtain Chinese medicinal decoction.
The decoction was used in the same manner as in example 1.
Example 3
The formula is as follows: 7 parts of bighead atractylodes rhizome, 7 parts of poria cocos, 8 parts of coix seed, 4 parts of rhizoma atractylodis, 2 parts of rheum officinale, 2 parts of rhizoma alismatis, 3 parts of dried orange peel, 3 parts of fructus aurantii, 3 parts of eupatorium and 3 parts of rhizoma cyperi.
The preparation method of the decoction comprises the following steps: (1) weighing the traditional Chinese medicine raw materials according to the parts by weight, smashing the raw materials, uniformly mixing the raw materials to form traditional Chinese medicine mixed powder, and weighing the traditional Chinese medicine mixed powder according to the dosage of 2 g per kilogram of body weight of a receptor for later use; (2) putting the weighed mixed powder of the traditional Chinese medicines into a ceramic container, adding clear water with the volume 4 times of the volume of the mixed powder of the traditional Chinese medicines according to the volume of the mixed powder of the traditional Chinese medicines, soaking for 30 minutes, boiling with strong fire, then slowly decocting with slow fire for 15 minutes, and filtering to obtain primary medicinal juice; rehydrating 2 times the original Chinese medicinal powder by volume, decocting with strong fire for 20 min, standing, naturally cooling to room temperature, filtering to obtain secondary medicinal liquid, and mixing the primary medicinal liquid and the secondary medicinal liquid to obtain Chinese medicinal decoction.
The decoction was used in the same manner as in example 1.
Example 4
The formula is as follows: 9 parts of bighead atractylodes rhizome, 9 parts of poria cocos, 9 parts of coix seed, 6 parts of rhizoma atractylodis, 3 parts of rheum officinale, 3 parts of rhizoma alismatis, 5 parts of dried orange peel, 5 parts of fructus aurantii, 5 parts of herba eupatorii and 5 parts of rhizoma cyperi.
The preparation method of the decoction is the same as that of the example 2.
The decoction was used in the same manner as in example 1.
Example 5
The formula is as follows: 12 parts of bighead atractylodes rhizome, 12 parts of poria cocos, 12 parts of coix seed, 8 parts of rhizoma atractylodis, 6 parts of rheum officinale, 6 parts of rhizoma alismatis, 6 parts of dried orange peel, 6 parts of fructus aurantii, 6 parts of eupatorium and 6 parts of rhizoma cyperi.
The preparation method of the decoction is the same as that of the example 3.
The decoction was used in the same manner as in example 1.
Examples of the experiments
1 materials and methods
1.1 test animals and breeding management select 240 healthy Wuhua three-yellow hens of 1 day old with uniform weight. They were randomly and uniformly divided into 6 groups: control and five test treatment groups of 4 replicates each with 10 chickens per replicate. The control group was fed with the normal complete formula feed, and the five groups of test groups were fed with the decoctions prepared in the above examples 1 to 5 on the basis of the normal complete formula feed, respectively, and the test period was 120 days.
All test chickens are immunized according to an immunization routine procedure, a carton flat-breeding feeding mode is adopted in the brooding period, the chicks are fed with water and water freely, heat preservation is carried out by adopting light of a bulb for 23 hours, and the chicks are fed in 4 stages of 0-2 weeks old, 3-6 weeks old, 7-14 weeks old and 15-17 weeks old. The feed is administered 6 times per day at 0-6 weeks of age. Transferring the young pigs to a breeding period after 6 weeks of age, and performing cage culture after the young pigs enter the breeding period. 1 time of feeding in the morning, in the middle and at night. The health of the flock was observed daily regularly during the test period and recorded.
1.2 test feed the basic diet composition and nutritional level of the test chickens are shown in table 1.
Table 1 basal diet ratio (%)
1.3 sample collection day 30, 60, 90, 120 days old 5 chickens with moderate weight and healthy body conditions were randomly selected from the control group and the test group, were prohibited from feeding (water is prohibited) twelve hours before slaughtering, weighed, blood was collected from the fasting heart for 2mL (heparin sodium anticoagulation), centrifuged at 4 ℃ and 3000r/m for 10min, and the supernatant was removed and transferred to an EP tube and stored in a refrigerator at-20 ℃ for later use to measure the biochemical indices (TG, VLDL) of blood. Slaughtering, weighing liver, cutting part of liver tissue, rapidly grinding on ice to form slurry, extracting total RNA in the liver tissue by using an animal tissue RNA extraction kit (Fuji organisms, Chengdu), and detecting the real-time expression quantity of a target gene by fluorescence quantitative PCR, wherein the quantitative gene and a primer sequence thereof in the research are shown in Table 2.
TABLE 2 PCR primer sequences
Note that in the above table, the influence of genes FAS and β -actin on the growth performance of Luhua chicken and the expression of FAS and ApoB genes in the tissues [ D ] Changsha, Hunan agriculture university, 2015 ] and the gene LPL are detailed in the Wangjing, Guangxi Sanhuang chicken character analysis and the correlation study of the expression of LPL and H-FABP genes and the intramuscular fat content [ D ]. Nanning, Guangxi university, 2013 ]
1.4 detection indexes and methods for content determination of Triglyceride (TG), Total Cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) in serum are carried out according to the instruction of a kit, and the kit is purchased from Nanjing Biotechnology engineering company.
1.5 data processing all data processing and analysis the statistical software SPSS 22.0 was used for one-way anova and multiple comparisons by Duncan's method, the results were expressed as mean ± standard error, with P <0.05 as the criterion for significance of difference.
2 results of the experiment
2.1 Effect of Compound Chinese herbal medicine preparation on blood lipid level of Sanhuang chicken
As shown in table 3, the total cholesterol and hdl cholesterol levels in the serum of the wuhua sanhuang chickens increased with the age of day during the whole experiment, and at the same age of day, the total cholesterol level in the experimental group 3 was significantly lower than that in the control group (P <0.05), while the hdl cholesterol level in the experimental group was higher than that in the control group (P < 0.05); the content of triglyceride in the serum of each treatment group is in a descending trend during the experiment, particularly the difference between the experiment group 3 and the control group is obvious, but the change of low-density lipoprotein cholesterol in each group is not obvious during the whole experiment, the difference is not obvious when the experiment group is slightly lower than the control group (P is more than 0.05).
TABLE 3 influence of compound Chinese herbal medicine preparation on serum biochemical index of Wuhua Sanhuang chicken
Note: the above test groups 1 to 5 were fed with the decoction prepared in examples 1 to 5, respectively. The different lower case letters of the shoulder marks of the same row indicate that the difference in the group is significant (P <0.05), and the no letters or the same lower case letters indicate that the difference in the group is not significant (P > 0.05); the same column of shoulder marks with different capital letters indicates significant difference between groups (P <0.05), and the letters or the same capital letters indicate insignificant difference between groups (P > 0.05); the following table is the same.
2.2 Effect of Compound Chinese herbal medicine preparation on the expression quantity of FAS and LPL gene mRNA of Wuhua Sanhuang chicken liver
2.2.1 RNA extraction results Total RNA was extracted according to the kit, and 1% agarose gel electrophoresis was used to detect the integrity of RNA, the detection results are shown in FIG. 1, it is clear that there are three bands 28S, 18S and 5S in the figure, indicating that the RNA structure is complete, and the OD260/OD280 value is greater than 1.8, so it can be used as the template for RT-PCR in the subsequent experiments.
2.2.2 fluorescent quantitation of PCR results
2.2.2.1 Standard and melting curves for the gene of interest and β -actin are shown in FIG. 2.
The copy number of a sample is obtained by comparing a Ct value with a standard curve, a relative quantification mode is adopted, namely target gene copies/internal reference copies (F value) is used for expressing test data, the test takes β -actin gene as an internal reference, a standard curve is constructed in a template relative concentration gradient range of 5-1-5-5, the amplification efficiency corresponding to the internal reference gene β -actin is 104.26%, the consistency coefficient R2 is 0.9991, the amplification efficiency corresponding to the target gene FAS is 100.40%, the consistency coefficient R2 is 0.9968, the amplification efficiency corresponding to the target gene LPL is 97.44%, the consistency coefficient R2 is 0.9999, and the peak value of a dissolution curve is stable and single and has no impurity peak (figure 2).
2.2.2.2 detection of expression level of FAS and LPL genes in liver tissue of Wuhua chicken
Table 4 shows statistics of the expression levels of FAS and LPL genes in the livers of chickens of different feeding days. As can be seen from the table, the expression level of FAS gene in the liver of each group of chickens is lower than that of the control group, especially the experimental group 3(P <0.05), but the expression level of FAS gene increases along with the growth of the chickens; and the expression of the LPL gene in the chicken liver of the experimental group is higher than that of the chicken liver of the control group, particularly the difference is obvious (P is less than 0.05) during the period of feeding the chickens within 31-59d, wherein the difference between the traditional Chinese medicine treatment group 3 and the control group is most obvious.
TABLE 4 influence of Chinese herbal medicine preparations on liver FAS and LPL gene mRNA expression
Discussion of 3
Fat is widely distributed in the living body, and has an extremely important biological function for maintaining the vital activities of the living body. The level of the related indexes of lipid metabolism in vivo reflects the lipid metabolism condition of the organism, and meanwhile, the level of lipid metabolism of poultry is closely related to the activity and content of lipometabolism enzymes. The Chinese herbal medicines are rich in alkaloid, organic acid and other bioactive substances, and after different Chinese herbal medicines are compounded and matched, a synergistic effect is generated, the metabolism of substances in a body can be regulated, and some trace elements are also activators of enzyme.
Test results show that the preparation can reduce the contents of TG, TC and LDL-C in the serum of a test group to different degrees compared with a control group, obviously improve the content of HDL-C in the serum (P is less than 0.05), is approximately the same as the existing research results, and shows that the components of the formula can effectively regulate the lipid metabolism of Wuhua Sanhuang chicken and improve the blood lipid level.
A large number of researches show that FAS is a main effective gene for regulating and controlling fatty acid synthesis, and is expressed in tissues such as liver and abdomen in a large amount, fatty acid synthetase reduces acetyl coenzyme A into fatty acid in liver, and the product is combined with glycerol to form TG. The existing research finds that the expression level of FAS in tissues such as liver and the like is reduced, and the blood lipid content of TG, TC and the like in serum can be effectively reduced. In the experiment, the expression level of FAS in the liver tissue of the Wuhua Sanhuang chicken fed with the compound Chinese herbal medicine preparation is obviously lower than that of a control group (P <0.05), the TG content in plasma of the Wuhua Sanhuang chicken is obviously lower than that of the control group (P <0.05), and the TG content in the plasma and the expression level of FAS in the liver show a positive correlation relationship, which indicates that the prescription can effectively inhibit the expression of FAS gene in the liver tissue, reduce the synthesis of fatty acid in the liver and regulate and control the fat deposition of animals. LPL is a rate-limiting enzyme in the hydrolysis of plasma lipoproteins to transport lipids to peripheral tissues, and its physiological function is primarily to hydrolyze triglycerides in Very Low Density Lipoproteins (VLDL), which are responsible for fat storage.
After the Chinese herbal medicine compound preparation is added into the experimental chicken daily feed, the expression level of LPL in the liver shows a trend of increasing firstly and then decreasing along with the increase of the adding time, which shows that the Chinese herbal medicine compound preparation can improve the lipid metabolism capability of the liver by enhancing the activity of LPL.
4 conclusion
The research shows that the compound Chinese herbal medicine preparation can effectively regulate the lipid metabolism of Wuhua Sanhuang chicken, can obviously reduce the gene expression of fatty acid synthetase FAS in liver tissues and can obviously regulate the expression of lipoprotein esterase LPL gene in the liver tissues, improves the lipid metabolism capability of the liver, and has a certain regulation and control effect on the fat deposition in organisms.
The above-mentioned embodiments are only for convenience of description, and are not intended to limit the present invention in any way, and those skilled in the art will understand that the technical features of the present invention can be modified or changed by other equivalent embodiments without departing from the scope of the present invention.
Claims (6)
1. A traditional Chinese medicine composition for reducing intramuscular fat of chicken is characterized by being prepared from the following traditional Chinese medicine raw materials in parts by weight: 4-12 parts of bighead atractylodes rhizome, 4-12 parts of poria cocos, 4-12 parts of coix seed, 2-8 parts of rhizoma atractylodis, 0.5-6 parts of rheum officinale, 0.5-6 parts of rhizoma alismatis, 0.5-6 parts of dried orange peel, 0.5-6 parts of fructus aurantii, 0.5-6 parts of eupatorium and 0.5-6 parts of rhizoma cyperi.
2. The traditional Chinese medicine composition for reducing the intramuscular fat of the chicken as claimed in claim 1, which is prepared from the following traditional Chinese medicine raw materials in parts by weight: 5-9 parts of bighead atractylodes rhizome, 5-9 parts of poria cocos, 5-9 parts of coix seed, 3-6 parts of rhizoma atractylodis, 1-3 parts of rheum officinale, 1-3 parts of rhizoma alismatis, 1-5 parts of dried orange peel, 1-5 parts of fructus aurantii, 1-5 parts of eupatorium and 1-5 parts of rhizoma cyperi.
3. The traditional Chinese medicine composition for reducing the intramuscular fat of the chicken according to claim 1 or 2, which is characterized by being prepared from the following traditional Chinese medicine raw materials in parts by weight: 7 parts of bighead atractylodes rhizome, 7 parts of poria cocos, 8 parts of coix seed, 4 parts of rhizoma atractylodis, 2 parts of rheum officinale, 2 parts of rhizoma alismatis, 3 parts of dried orange peel, 3 parts of fructus aurantii, 3 parts of eupatorium and 3 parts of rhizoma cyperi.
4. A method of preparing a composition as claimed in any one of the preceding claims, comprising the steps of:
(1) weighing the traditional Chinese medicine raw materials according to the parts by weight, smashing the raw materials, uniformly mixing the raw materials to form traditional Chinese medicine mixed powder, and weighing the traditional Chinese medicine mixed powder according to the dosage of 0.8-2 g of receptor per kilogram of body weight for later use; (2) putting the weighed mixed powder of the traditional Chinese medicines into a ceramic container, soaking and decocting into decoction.
5. The preparation method of the traditional Chinese medicine composition according to claim 4, wherein the step (2) is specifically as follows: putting the weighed mixed powder of the traditional Chinese medicines into a ceramic container, adding clear water with the volume of 3-5 times of the volume of the mixed powder of the traditional Chinese medicines according to the volume of the mixed powder of the traditional Chinese medicines, soaking for 30 minutes, boiling with strong fire, then slowly decocting for 10-15 minutes with slow fire, and filtering to obtain primary medicine juice; rehydrating 1-2 times the original Chinese medicinal powder by volume, decocting with strong fire for 15-20 min, standing, naturally cooling to room temperature, filtering to obtain secondary medicinal liquid, and mixing the primary medicinal liquid and the secondary medicinal liquid to obtain Chinese medicinal decoction.
6. A method of using the Chinese medicinal composition of claim 4 or 5, wherein the method comprises: the water supply of the chickens is stopped for two hours before the administration, then the total amount of water required by the chickens to be fed with the medicine is calculated according to the drinking amount of each chicken for two hours, and the decoction is mixed with water according to the drinking amount to obtain the liquid medicine which is taken once a day.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1171236A (en) * | 1996-07-18 | 1998-01-28 | 颜培克 | Weight-lossing essence for human body |
| CN1231900A (en) * | 1998-04-10 | 1999-10-20 | 宋天灵 | Weight-loss medicine and its preparing method |
| CN1398615A (en) * | 2002-07-17 | 2003-02-26 | 崔兴中 | Chinese medicine prepn for treating fatty liver and obesity |
| CN101028463A (en) * | 2007-04-16 | 2007-09-05 | 北京艺信堂医药研究所 | Health-care product with diet function |
| CN102028900A (en) * | 2010-06-12 | 2011-04-27 | 中山大学附属第一医院 | Traditional Chinese medicine for treating metabolic syndrome and preparation method thereof |
| CN107126525A (en) * | 2017-04-26 | 2017-09-05 | 合肥海大生物医药科技有限公司 | It is a kind of to treat Chinese medicine composition of simple obesity and preparation method thereof |
| CN110368439A (en) * | 2019-08-29 | 2019-10-25 | 河南珍珠练生物科技有限公司 | A kind of Chinese medicine health slimming side |
-
2020
- 2020-04-01 CN CN202010248271.4A patent/CN111228422B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1171236A (en) * | 1996-07-18 | 1998-01-28 | 颜培克 | Weight-lossing essence for human body |
| CN1231900A (en) * | 1998-04-10 | 1999-10-20 | 宋天灵 | Weight-loss medicine and its preparing method |
| CN1398615A (en) * | 2002-07-17 | 2003-02-26 | 崔兴中 | Chinese medicine prepn for treating fatty liver and obesity |
| CN101028463A (en) * | 2007-04-16 | 2007-09-05 | 北京艺信堂医药研究所 | Health-care product with diet function |
| CN102028900A (en) * | 2010-06-12 | 2011-04-27 | 中山大学附属第一医院 | Traditional Chinese medicine for treating metabolic syndrome and preparation method thereof |
| CN107126525A (en) * | 2017-04-26 | 2017-09-05 | 合肥海大生物医药科技有限公司 | It is a kind of to treat Chinese medicine composition of simple obesity and preparation method thereof |
| CN110368439A (en) * | 2019-08-29 | 2019-10-25 | 河南珍珠练生物科技有限公司 | A kind of Chinese medicine health slimming side |
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