CN111228186A

CN111228186A - Composition for preventing alopecia and promoting hair growth and application

Info

Publication number: CN111228186A
Application number: CN202010251231.5A
Authority: CN
Inventors: 何瀛; 陈恺
Original assignee: Shanghai Kezhi Beixin Daily Chemical Technology Co Ltd
Current assignee: Shanghai Kezhi Beixin Daily Chemical Technology Co Ltd
Priority date: 2020-04-01
Filing date: 2020-04-01
Publication date: 2020-06-05
Anticipated expiration: 2040-04-01
Also published as: CN111228186B

Abstract

The invention relates to a composition for preventing alopecia and promoting hair growth and application thereof, wherein the composition for preventing alopecia and promoting hair growth comprises the following raw material components: hydrolyzed lupin protein, yeast extract, Glycyrrhrizae radix extract, folium Platycladi extract, and hydrolyzed sericin. The composition for preventing alopecia and growing hair can provide necessary trace element nutrition for each stage of hair growth, shorten the process of the hair from a resting stage to a growing stage, delay the conversion from the growing stage to a retreating stage, effectively repair scalp hair follicle injury, promote hair growth, nourish and fix hair and prevent hair from falling off. The traditional Chinese medicine has obvious effect on alopecia caused by various reasons, and has the characteristics of quick response, wide applicable population and mild effect.

Description

Composition for preventing alopecia and promoting hair growth and application

Technical Field

The invention belongs to the technical field of daily chemicals, and particularly relates to a composition for preventing alopecia and growing hair and application thereof.

Background

The growth of hair is periodic and divided into growth phase, catagen phase and telogen phase. (1) And (3) growing period: the growing hair grows for 2-5 years, for 0.27-0.40mm per day, characterized by continuous growth, and then enters the catagen. (2) A return period: at this point the hair stops growing, is shed easily for several days to several weeks, and then enters a resting period. (3) Rest period: the telogen phase typically lasts 3-4 months until a new follicular cycle begins. Under normal conditions, more than 90% of hair is in the growth phase, 10% of hair is in the resting phase, only 1% of hair is in the catagen phase, which is the normal metabolism of hair follicles, normal people fall off less than 100 hairs every day, the phenomenon is normal, large-area sparsity cannot be caused, and the hairs can enter the growth phase again after falling off. If a problem occurs during this process, the follicle does not circulate properly: for example, hair follicles fall off after a resting period, but cannot enter a growing period again, and a large number of the hair follicles are present, so that hair is sparse, and hair loss occurs. There are many factors that cause the follicle to no longer enter anagen phase: malnutrition, hormones, stress, chemical or physical effects, drugs, genetics, etc.

The Chinese scalp health white paper shows that about 2 hundred million people suffering from alopecia in China, the market penetration rate of the hair care and treatment industry is estimated to reach 20% in 2020, and the economic scale of the whole hair care and treatment industry and related industries is expected to exceed 400 hundred million yuan. Data show that the Chinese alopecia has high incidence rate and rapidly develops at a growth rate of 15 to 18 percent each year. The number of people in the Chinese adult alopecia population is more, particularly in the male alopecia population, according to the data of the world health organization, 1 in every 5 Chinese men has alopecia symptoms, and in the alopecia population, the alopecia accounts for 84% before the age of 30, and the alopecia is more and more generalized and has a low-age trend.

In recent years, in scalp care, alopecia prevention and hair loss prevention become the rigid requirements of individual crowds, products for alopecia prevention and hair growth are more on the market, however, the effects of alopecia prevention and hair growth are not ideal, the range of applicable crowds is narrow, and the differentiation of the using effects of the crowds is obvious, so that the development of an alopecia prevention and hair growth product which is quick in alopecia prevention and hair growth effect, suitable for most crowds at the present stage and high in use safety and comfort has very important practical significance and wide market application prospect.

Disclosure of Invention

The invention aims to provide an anti-hair loss and hair growth composition and application, wherein the anti-hair loss and hair growth composition is prepared by combining 4 natural source components, can act on the period of hair growth, and plays an excellent role in hair growth and hair solidification.

The technical scheme of the invention is as follows:

the invention provides a composition for preventing alopecia and growing hair, which comprises the following raw material components: hydrolyzed lupin protein, yeast extract, Glycyrrhrizae radix extract, folium Platycladi extract, and hydrolyzed sericin.

The hydrolyzed lupin protein added in the invention is rich in glutamic acid, peptide, trace protein and vitamin, acts on hair growth cycle mainly by regulating 3 aspects of hormone balance, vascular system and cell metabolism, 1, androgenetic alopecia is an important factor causing alopecia, the sensitivity of local scalp hair follicles of a patient to 5 α -DHT is increased, leading to hair follicle miniaturization, hair shaft thinning, and clinical manifestation of hair thinning and thinning, 5 α -DHT is transformed by testosterone under the action of 5 α -reductase II, the added hydrolyzed lupin the invention has the effect of inhibiting the activity of 5 α -reductase II, thus inhibiting the production of 5 α -DHT, thereby reducing androgenetic alopecia caused by 5 α -DHT, 2, the hair follicle has a very important role for normal growth of hair, especially for hair in growth phase, the hair contains growth factors, cytokines and other molecules required for regulating growth cycle, cytokines and other molecules, wherein VEGF is a known important vascular endothelial factor, is a vascular system which is a high vascular permeability promoting factor for hair growth, thus the growth factor can promote hair cell proliferation, cell proliferation and cell proliferation of hair growth cycle formation of hair cells are increased, cell proliferation is an important factor for promoting hair growth, the invention, the aspects of hair growth factor, the invention can promote hair growth cycle, the hair growth factor is capable of promoting hair growth, the growth of hair growth, the cell growth factor is capable of promoting hair growth, the cell growth of promoting hair growth, the hair growth of hair growth, the cell growth of promoting hair growth, the hair growth factor of promoting hair growth, the hair growth of hair growth, the growth of hair growth, the hair growth of hair growth, the hair growth of hair is capable of promoting hair growth of promoting hair growth of hair, the hair growth of promoting hair growth of.

Another component of the composition of the invention, yeast extract, provides a synergistic effect with the other components, mainly from 4 aspects: 1. mitochondrial dynamics aspects: the growth of hair follicles is a process requiring a large supply of energy, the energy production in cells is provided by mitochondria, which behave as a highly dynamic organelle within the cell, maintaining mitochondrial homeostasis, i.e., mitochondrial dynamics, by constantly dividing and fusing. Compared to fission, the fusion process between mitochondria with the involvement of mitochondrial outer membrane fusion protein 1(MFN1) enables organelles to enhance their metabolism and provide more energy in the form of ATP. The supply of this energy is to the hair in the anagen phase and is thus of great concern for maintaining the entire hair growth cycle. In addition, mitochondria are not only major energy-producing organelles in the cell, but also regulate the production of active oxygen, the production of free radicals, and the like. The data show that the results of tests performed on human hair follicle dermal papilla cells indicate that 0.04% yeast extract is able to increase the synthesis of MFN1 by 52%, indicating that yeast extract plays a very positive role in promoting mitochondrial fusion and, in turn, providing the energy supply required for hair follicle cell growth. 2. Aspect of signal molecule expression: signal molecules in the microenvironment of the papilla are also important for hair growth, and overexpression of 3 signal molecules including IL-6, DKK1, P16, which are currently known, is closely associated with androgenic alopecia. The data show that DHT treated human hair follicle dermal papilla cells tested in vitro with 0.04% yeast extract reduced IL-6 secretion by 97% and DKK1 and P16 expression by 91% and 71%, respectively, suggesting that yeast extract plays a role in correcting aberrant expression of signaling molecules responsible for androgenetic alopecia. 3. Epigenetic aspect: research has shown that iRNA plays an important role in hair biology, and is involved in regulating proliferation, differentiation and apoptosis of dermal papilla fibroblasts and hair keratinocytes. 3663-3p and let-7a-3p miRNA overexpression is associated with hair loss formation, and data show that DHT-treated human hair follicle dermal papilla cell in vitro tests show that 0.04% yeast extract limits 3663-3p and let-7a-3p miRNA expression by 50% and 89%, respectively, which indicates that yeast extract plays a positive role in reducing epigenetic disorders causing hair loss. 4. Dermal papilla activity: proteoglycans are characteristic markers of the hair in the anagen phase, the reduction of which suggests a shortening of the anagen phase, tests on 3D models of human hair follicle dermal papilla cells show that 0.04% yeast extract promotes proteoglycan synthesis. After treatment of the hair follicles with DHT, the yeast extract increased proteoglycan by 37% after 7 days and remained continuously synthesized, i.e., the yeast extract reduced the risk of hair loss by keeping the hair follicles in the anagen phase all the time, suggesting that it plays an important role in maintaining dermal papilla activity and promoting hair growth.

The composition also comprises a licorice extract, a cacumen biotae extract and hydrolyzed sericin, wherein the main active ingredients of the licorice extract comprise glycyrrhizic acid, ammonium glycyrrhizinate, sodium glycyrrhetinate, licorice flavonoids, semaphorin, formononetin, quercetin and the like, and the licorice extract is yellow to brown yellow powder. The Glycyrrhrizae radix extract has certain catalytic effect on antihistaminic and vasodilatation in human body, and can relieve skin inflammation reaction due to various skin appearance. Wherein glycyrrhizic acid has inhibitory effect on Gossypium granuloma, formaldehyde edema, tuberculin reaction, and subcutaneous granulomatosis of white rat. Ammonium glycyrrhizinate and sodium glycyrrhetinate can effectively influence the exudation phase and the proliferation phase of subcutaneous granulomatous inflammation, and the action intensity of the ammonium glycyrrhizinate and the sodium glycyrrhetinate is weaker than or close to that of cortisone. In addition, the licorice extract can effectively prevent the formation of melanin by inhibiting the activity of tyrosinase and dopachrome tautomerase (TRP-2), thereby having a certain skin whitening effect.

The cacumen biotae extract is branch tips and leaves of Franco of Platylladus orientalis (L.) of Cupressaceae, and is regarded as bitter and cold in Chinese medicine, enters liver channel, and liver governs wind, mainly stores blood, and hair is the rest of blood, so that the cacumen biotae extract can cool blood and blacken hair, and can be used for blood heat alopecia, early white beard and hair and the like. Modern researches show that the cacumen biotae extract has a strong inhibition effect on the biosynthesis of leucocyte LTB4 and 5-HETE (5-lipoxygenase), and also shows a strong inhibition effect on cyclooxygenase, which indicates that the cacumen biotae extract has a certain anti-inflammatory effect by inhibiting the activity of 5-lipoxygenase and the components of cyclooxygenase.

Sericin is similar to natural moisture regulating factors in human skin horny layer, can keep proper amount of water in skin to make skin smooth, soft and elastic, sericin and hydrolysate thereof are natural antioxidant, and the capability of inhibiting fat peroxidation is comparable with VC. It also has tyrosinase activity inhibiting effect. In addition, the water-soluble sericin and the hydrolysate thereof have obvious curative effect on certain skin diseases, have the function of skin inflammation diminishing, simultaneously promote the generation of collagen of dermal fiber cells, and effectively prevent or slow down various skin diseases such as skin wrinkling, aging, elasticity reduction, roughness, blackening, relaxation and the like.

Preferably, the raw material components comprise the following components in parts by weight: 1-10 parts of hydrolyzed lupin protein, 0.1-0.3 part of yeast extract, 1-5 parts of licorice extract, 5-10 parts of cacumen biotae extract and 1-5 parts of hydrolyzed sericin.

Preferably, the raw material components comprise the following components in parts by weight: 5 parts of hydrolyzed lupin protein, 0.2 part of yeast extract, 3 parts of liquorice extract, 8 parts of cacumen biotae extract and 3.6 parts of hydrolyzed sericin.

A hair-growing essence containing the hair-loss-preventing and hair-growing composition comprises the following raw material components:

57-60 parts of water, 13-17 parts of humectant, 1-3 parts of freshener, 4.7-6.2 parts of skin conditioner, 0.2-0.3 part of preservative, 0.03-0.08 part of chelating agent and 16.3-22.1 parts of anti-alopecia and hair-growing composition.

Preferably, the humectant is one or two of propylene glycol and glycerol.

Preferably, the cooling agent is ethanol.

Preferably, the skin conditioning agent is a mixture of panthenol, dipotassium glycyrrhizinate, and niacinamide.

Preferably, the skin conditioner is a mixture of panthenol, dipotassium glycyrrhizinate and niacinamide in a weight ratio of 1.2-2.4:0.8-1.5: 2-3. The skin conditioner composition provided by the invention can be matched with a composition for preventing alopecia and promoting hair growth, and provides the best use feeling.

The composition has multiple purposes, can be used in various hair care products such as shampoo, hair moisturizing cream, hair nourishing essence, hair follicle nutrient solution and the like, and more preferably, the addition amount of the composition for preventing alopecia and growing hair is 10-30 wt%.

The invention has the beneficial effects that:

the composition for preventing hair loss and growing hair provided by the invention has the advantages that 4 raw materials of hydrolyzed lupin protein, yeast extract, licorice extract, cacumen biotae extract and hydrolyzed sericin are combined and reasonably proportioned, so that the 4 raw materials are complementary in the mechanism of preventing hair loss and play a synergistic effect. The traditional Chinese medicine has obvious effect on alopecia caused by various reasons, and has the characteristics of quick response, wide applicable population and mild effect.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention. In the following examples, 1 part by weight represents 1 g.

Example 1

The embodiment provides a composition for preventing alopecia and growing hair, which comprises the following raw materials in parts by weight:

5 parts of hydrolyzed lupin protein, 0.2 part of yeast extract, 3 parts of liquorice extract, 8 parts of cacumen biotae extract and 3.6 parts of hydrolyzed sericin.

The preparation method of the composition for preventing alopecia and promoting hair growth, which is disclosed by the embodiment, comprises the following specific steps: respectively taking hydrolyzed lupin protein, yeast extract, Glycyrrhrizae radix extract, folium Platycladi extract, and hydrolyzed sericin, mixing and stirring well to obtain the final product.

Example 2

1 part by weight of hydrolyzed lupin protein, 0.3 part by weight of yeast extract, 5 parts by weight of licorice extract, 5 parts by weight of cacumen biotae extract and 5 parts by weight of hydrolyzed sericin.

Example 3

10 parts of hydrolyzed lupin protein, 0.1 part of yeast extract, 1 part of liquorice extract, 10 parts of cacumen biotae extract and 1 part of hydrolyzed sericin.

Example 4

The embodiment provides a hair-growing essence which is prepared from the following raw materials in parts by weight:

58 parts of water, 5 parts of propylene glycol, 10 parts of glycerol, 2 parts of ethanol, 1.8 parts of panthenol, 1.2 parts of dipotassium glycyrrhizinate, 2.5 parts of nicotinamide, 0.3 part of methylparaben, 0.05 part of disodium EDTA and 19.8 parts of the hair loss preventing and hair growing composition in example 1.

The preparation method of the composition for preventing alopecia and promoting hair growth, which is disclosed by the embodiment, comprises the following specific steps: adding methyl hydroxybenzoate into ethanol, mixing and stirring to dissolve uniformly to obtain a solution 1; mixing and stirring the rest raw materials to dissolve uniformly to obtain a solution 2; and uniformly mixing the solution 1 and the solution 2 to obtain the composite material.

Example 5

60 parts of water, 2 parts of propylene glycol, 15 parts of glycerol, 1 part of ethanol, 2.4 parts of panthenol, 0.8 part of dipotassium glycyrrhizinate, 3 parts of nicotinamide, 0.2 part of methyl hydroxybenzoate, 0.03 part of disodium EDTA and 16.3 parts of the hair loss preventing and hair growth promoting composition in example 2.

Example 6

57 parts by weight of water, 8 parts by weight of propylene glycol, 5 parts by weight of glycerin, 3 parts by weight of ethanol, 1.2 parts by weight of panthenol, 1.5 parts by weight of dipotassium glycyrrhizinate, 2 parts by weight of nicotinamide, 0.3 part by weight of methylparaben, 0.08 part by weight of disodium EDTA, and 22.1 parts by weight of the composition for preventing alopecia and growing hair in example 3.

Comparative example 1

The hair loss preventing and hair growing composition is different from the hair loss preventing and hair growing composition in example 4, and specifically, the hair loss preventing and hair growing composition added in the comparative example consists of the following raw materials in parts by weight: 5.2 parts of hydrolyzed lupin protein, 3 parts of liquorice extract, 8 parts of cacumen biotae extract and 3.6 parts of hydrolyzed sericin.

Comparative example 2

The hair loss preventing and hair growing composition is different from the hair loss preventing and hair growing composition in example 4, and specifically, the hair loss preventing and hair growing composition added in the comparative example consists of the following raw materials in parts by weight: 10 parts of hydrolyzed lupin protein and 0.5 part of yeast extract.

Effect of the present invention on mouse Hair growth

1. Test animals: 50C 57BL6 mice with hair growing into resting stage, each half of male and female, and the weight of 15-20 g;

2. the test method comprises the following steps: dividing all mice into 5 groups of a negative control group, a positive control group, a test group 1, a test group 2 and a test group 3, wherein each group comprises 10 mice, selecting a 3 x 3cm area on the back as a tested area after the mice in each group are slightly anesthetized, heating and melting a rosin/paraffin mixture (1:1), applying the mixture to the tested area when the temperature is proper, and tearing down the mixture after solidification to pluck hairs so as to induce the hairs of the mice to enter a growth period from a resting period. The samples were applied evenly to the depilatory area of each group, 0.2ml of the essence vehicle base (the composition of example 4 was replaced with water) was given to the negative control group, and equal volumes of minoxidil (3 wt%) and hair conditioner of examples 4-6 were given to the positive control group and the test groups 1-3, respectively, 2 times per day for 28 days. The hair growth cycle was observed at the plucked area and the time required for the skin color at the pluck to change from pink (telogen) to black (anagen) and from black (anagen) to gray (catagen) was recorded. On the last day of application of the samples, 10 new hairs were randomly picked from each mouse at the plucking area, the length was measured with a vernier caliper, the average hair length was calculated, and all the new hairs at the plucking area were weighed.

3. The test results are shown in tables 1 and 2.

TABLE 1 Effect on the Hair growth cycle

Group of	Time required from resting period to growing period/d	Time required from growth phase to withdrawal phase/d
			Negative control group	6.23±0.26	11.15±1.37
Positive control group	4.78±0.18^**	15.32±1.24^**
			Test group 1	5.06±0.15^**	15.28±1.06^**
Test group 2	6.08±0.09	12.34±0.98
			Test group 3	6.11±0.12	12.25±1.15

Compared with the blank control group, the ratio of the active ingredients,^*p<0.05,^**p<0.01

TABLE 2 Effect on Hair Length

Group of	Average hair length/mm	Total weight of fresh hair/mg
			Negative control group	5.72±0.63	2.51±0.33
Positive control group	7.14±0.42^##	7.09±0.21^##
			Test group 1	7.12±0.37^##	7.08±0.18^##
Test group 2	5.96±0.51	2.59±0.24
			Test group 3	5.85±0.46	2.55±0.27

Comparison with blankThe ratio of the groups is determined,^#p<0.05,^##p<0.01

the results show that compared with the negative control group, the time required by the hair of the mouse using the sample of the invention from the resting stage to the growing stage is obviously shortened, the time required by the hair of the mouse from the growing stage to the catagen stage is obviously prolonged, the average hair length and the total weight of new hair are obviously increased (P <0.01), the effect is obviously better than that of the sample of the comparative example, and the effect is equivalent to that of the positive control group. The sample of the invention can affect the hair growth cycle, shorten the time required by the hair of the mouse to enter the growth phase from the resting phase and prolong the growth phase of the hair, thereby having the obvious effect of promoting the hair growth.

The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.

Claims

1. The composition for preventing alopecia and growing hair is characterized by comprising the following raw materials: hydrolyzed lupin protein, yeast extract, Glycyrrhrizae radix extract, folium Platycladi extract, and hydrolyzed sericin.

2. The composition for preventing hair loss and growing hair according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 1-10 parts of hydrolyzed lupin protein, 0.1-0.3 part of yeast extract, 1-5 parts of licorice extract, 5-10 parts of cacumen biotae extract and 1-5 parts of hydrolyzed sericin.

3. The composition for preventing hair loss and growing hair according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 5 parts of hydrolyzed lupin protein, 0.2 part of yeast extract, 3 parts of liquorice extract, 8 parts of cacumen biotae extract and 3.6 parts of hydrolyzed sericin.

4. A hair tonic comprising the composition for preventing hair loss and growing hair according to any one of claims 1 to 3, wherein the raw material components comprise:

5. The hair tonic according to claim 4, wherein the humectant is either one or both of propylene glycol and glycerin.

6. The hair growth shampoo of claim 4 wherein the cooling agent is ethanol.

7. The hair tonic according to claim 4, wherein the skin conditioner is a mixture of panthenol, dipotassium glycyrrhizinate and niacinamide.

8. The hair growth shampoo of claim 7 wherein the skin conditioner is a mixture of panthenol, dipotassium glycyrrhizinate, and niacinamide in a weight ratio of 1.2-2.4:0.8-1.5: 2-3.

9. The composition for preventing hair loss and growing hair according to any one of claims 1 to 3, which is used in a shampoo, a hair cream, a hair-growing essence, and a hair follicle nutrient solution.

10. The use according to claim 9, wherein the alopecia preventing and hair growth promoting composition is added in an amount of 10-30 wt%.