CN116209425A

CN116209425A - Composition for preventing alopecia and promoting hair growth

Info

Publication number: CN116209425A
Application number: CN202180060665.XA
Authority: CN
Inventors: 申载永; 金载润; 崔允豪; 李相和; 康鎣美
Original assignee: LG Household and Health Care Ltd
Current assignee: LG H&H Co Ltd
Priority date: 2020-07-14
Filing date: 2021-07-14
Publication date: 2023-06-02
Also published as: WO2022015055A1; JP2023534287A; KR20220008790A

Abstract

The present disclosure relates to a composition for preventing hair loss and promoting hair growth, which contains an ingredient that induces hair growth promotion. The composition for preventing hair loss and promoting hair growth of the present disclosure promotes activity of papilla cells, mitochondrial activity of papilla cells and activity of Wnt/β -catenin signaling system through a combination between specific active ingredients, thereby producing a remarkable synergistic effect on the effects of preventing hair loss and promoting hair growth.

Description

Composition for preventing alopecia and promoting hair growth

Technical Field

The present application claims priority based on korean patent application No. 10-2020-0086878, filed on 7 months and 14 days in 2020, and the entire contents of the specification of the related application and the disclosure in the accompanying drawings are incorporated herein by reference.

The present invention relates to a composition for preventing hair loss and promoting hair growth.

Background

With the change of the age, hair occupies an important part in the process of increasing attention to beauty. Hair is a fine and keratinized structure produced on the surface of the skin. The multifunctional external shock absorber has the buffering effect on external shock, plays a role of protecting a human body from external stimuli such as direct light, cold, friction, danger and the like, and discharges heavy metals such as arsenic, mercury, zinc and the like harmful to the body to the outside, and has the beauty aspect of modern and emphasized decoration. However, there are increasing numbers of people complaining of hair loss for various reasons including changes in dietary life and increased internal and external stress. It is known that genetic factors are the most important cause of hair loss, but as described above, increase in social stress, environmental pollution, frequent permanent waving and dyeing, and erroneous scalp management are also becoming causes of hair loss.

Human hair is about 10-15 tens of thousands, each hair having a different growth cycle from the others. The formation of the hair follicle (hair follicle) occurs under the action of β -catenin to form a basal plate (placode) from basal epidermal cells (Sarah E.Miller, 2002). Dermal fibroblasts receiving signals from the formed substrate form skin aggregates (dermal condensate). At the same time, the substrate grows and sinks into the dermis and combines with the skin aggregate to form a primary blank (primary hair germ). The epidermal cells forming the hair embryo grow long in the direction of the dermis to form a long columnar structure composed of multiple layers, i.e., hair peg, and the skin aggregate is formed of a spherical hair papilla (dermal papilla). The distal end of the hair pin gradually becomes thicker to form hair bulb (hair bulb), and the hair bulb forms a form of wrapping the hair papilla, so that hair follicle is completed, and epidermal cells inside the hair follicle are differentiated to generate hair.

The initially generated hair circulates the following period: the hair enters the catagen stage and the telogen stage after the anagen stage, and then enters the anagen stage again after the hair falls off. The hair growth cycle consists of three phases, classified as the anagen phase (anagen stage) where hair growth is most active, the catagen phase (catagen stage) where hair begins to degenerate, and the telogen stage where hair growth is stopped or enters rest.

It is well known that in regulating the growth cycle of such hair, the activity of external root sheath cells (outer root sheath cell) including papilla cells and follicle stem cells constituting hair follicles, various cytokines and growth factors produced in these cells play an important role. For example, DKK-1 (Dickkopf-1) plays an important role in hair follicle growth inhibition and killing. On the other hand, it is well known that for periodic hair growth, regeneration of hair follicles and activation of stem cells must be achieved, which is achieved by Wnt/β -catenin production and activation. In addition, growth factors such as VEGF, IGF-1, FGF, etc. are known to promote the anagen phase of hair, and TGFb-1, EGF, etc. are known to induce the catagen phase of hair follicles.

In general, alopecia refers to an abnormal increase in the number of hair loss due to an increase in the number of hair loss in the catagen phase, which is caused by a decrease in the proportion of hair in the anagen phase and an increase in the telogen phase in such a cycle. Alopecia is a normal phenomenon, but in normal people, there are many hairs in anagen phase, whereas in people with ordinary alopecia, there are many hairs in telogen phase, so that macroscopic alopecia occurs. People suffering from alopecia are characterized by miniaturization of hair. With the progress of alopecia, the time of the growing period is shortened, the transition to the retrograde period and the resting period is promoted, and then the volume of the hair papilla is reduced, and the hair follicle is gradually miniaturized. Therefore, in order to treat alopecia, it is important to rapidly transition the telogen state hair follicle to anagen phase and lengthen the shortened anagen phase.

As described above, as causes of alopecia, there are proposed male hormonal effects, hypersecretion of sebum, poor blood circulation, scalp function decline due to peroxides, bacteria, etc., genetic factors, aging, stress, etc. However, it is in a state that the clear cause of hair loss has not been revealed so far, and it is known that interleaving has a very complex effect.

For such various and complicated causes of alopecia, known alopecia preventing products include, as active ingredients, ingredients for the purpose of promoting blood circulation, strengthening hair root function, moisturizing scalp effect, preventing dandruff effect, antioxidant effect, hair growth period prolonging effect, inhibiting male hormonal effect, etc., but the effects are in a state where no significant effect has been achieved so far, and there is also a problem of side effects.

On the other hand, adenosine is a constituent of amino acids produced in cells, and is a major component of cellular energy metabolism. Adenosine is a compound in which a base called adenine and a sugar called ribose are combined as one of the elements constituting the gene DNA. For adenosine, if three phosphates bind to a sugar, it becomes the main source of intracellular energy, namely ATP (adenosine-5' -triphosphate). Thus, it is known that adenosine is an important element that plays a decisive role in the smooth progress of all metabolism while constituting the body. Adenosine is an intracellular component, and thus has excellent stability and durability when penetrating the skin, and promotes synthesis of DNA and protein in the dermis layer, thereby acting in addition to improving skin wrinkles.

Nicotinamide (Niacinamide) is a vitamin B complex, which is present in the plant kingdom and widely distributed with niacin, and exists as a component of redox coenzyme NAD or NADP in the animal kingdom organism. Nicotinamide is a physiologically active amide of vitamin B3, also known as pyridine-3-carboxylic acid, which is also known to act as an anti-inflammatory material or antioxidant. Nicotinamide blocks migration of melanosomes, thereby blocking migration of synthesized melanin to keratinocytes, thereby reducing skin pigmentation, enhancing whitening effect. In addition, nicotinamide increases collagen synthesis and intercellular lipid synthesis, thereby contributing to skin moisturization and the like.

In addition, D-panthenol (dexpanthenol) is an alcohol form of vitamin B5 (Pantothenic acid), which may be referred to as provitamin (provitamin) B5. D-panthenol is immediately after being absorbed by the skin and converted into vitamin B5 for use. D-panthenol is widely used as a component of skin moisturizer, wrinkle improver, etc. in cosmetics and living goods, and is also used as an ointment for wound healing, skin burn, itch improvement, etc. In addition, D-panthenol is known as a humectant having permeability, and is known to have effects of conditioning skin and hair and increasing recovery of damaged hair.

In addition, polygonum multiflorum is a perennial herb belonging to the Polygonaceae family, which is a vine medicinal plant and its discipline names are varied as Pleuropterus multiflorus, polygonum multiflorum, fagopyrum multiflorum, reynoutria multiflora or Fallopia multiflora. It is well known that polygonum multiflorum extract is used as a tonic, a seminal agent, a menstrual flow-promoting agent, a blood-nourishing agent or an antitussive agent, etc., and is used for treating cold, hematemesis, neurasthenia or arthritis, etc.

In addition, houttuynia cordata (Houttuynia cordata) is known as a perennial herb of the family Saururaceae as a herb of the genus Fagopyrum, a heavy herb, houttuynia cordata or ten herbs. Herba Houttuyniae contains various essential oil components, and emits unique smell such as fishy smell, so it is called herba Houttuyniae. Herba Houttuyniae flowers mainly in 5-7 months, and is a herb for treating gonorrhea, enteritis, urinary tract infection, pneumonia or bronchitis.

In addition, zinc pyrithione (zinc pyrithione) has an antibacterial effect, thus preventing the proliferation of microorganisms on the scalp and skin. In particular, zinc pyrithione has a proliferation inhibiting effect on malassezia (malassezia) and other fungi, and thus plays a role in preventing seborrheic dermatitis and dandruff formation.

In addition, salicylic acid is a major component of aspirin, and has been widely used as an anti-inflammatory agent, an analgesic agent, or a antipyretic agent. Salicylic acid plays a role in removing skin cutin and softening cutin. Salicylic acid is mainly added to cosmetics such as cleansing foam (cleansing foam), and softens and exfoliates keratin, thereby softening pores. Thus, salicylic acid can help the removal of sebum from pores, keeping the skin clean. In addition, salicylic acid has antibacterial and anti-inflammatory effects, and can inhibit proliferation of scalp or skin microorganisms, thereby improving skin and scalp or preventing dandruff.

On the other hand, the current alopecia therapeutic agent of the coating agent approved by the U.S. Food and Drug Administration (FDA) is minoxidil (2, 4-diamino-6-piperidylpyrimidine-3-oxide, 2, 4-diamido-6-piperidinopyridine-3-oxide) only, but the effect thereof is not ideal at 32 to 48 weeks, 38% and 13% respectively, and has the disadvantage of poor hair treatment effect.

Therefore, there is a need for developing a composition excellent in preventing hair loss or promoting hair growth, but there is no research on what effect is produced in preventing hair loss or promoting hair growth when the above adenosine, D-panthenol, nicotinamide, polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione, salicylic acid and the like are combined.

Disclosure of Invention

Technical problem

The present disclosure addresses the problem of providing a composition for preventing hair loss or promoting hair growth, which has excellent effects of preventing hair loss and/or promoting hair growth.

Further, the present disclosure provides a hair or scalp product comprising the composition for preventing hair loss or promoting hair growth.

Technical proposal

The present inventors have made diligent efforts to improve the problems such as side effects and precautions in use of conventional hair loss remedies, and to develop a composition which is safe for the human body and can effectively act on hair loss prevention and hair growth promotion without the drawbacks such as weak hair loss prevention/hair growth promotion effects of hair loss/hair tonic. As a result, the present inventors have found that when specific effective components are further combined in a mixture of adenosine, D-panthenol and nicotinamide, a remarkable synergistic effect is produced in preventing hair loss and promoting hair growth.

Specifically, the present inventors confirmed that when any one or more selected from the group consisting of polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione and salicylic acid is further combined in a mixture of adenosine, D-panthenol and nicotinamide, a remarkable synergistic effect is produced on the effect of enhancing papilla cell activity (experimental example 1), the effect of promoting papilla cell mitochondrial activity (experimental example 2), the effect of promoting papilla cell activity signal (Wnt/β -catenin) (experimental example 3) by an in vitro (in-vitro) experiment. In addition, the present inventors confirmed that when the composition for preventing hair loss or promoting hair growth comprising the above-described combination is applied to a patient suffering from hair loss, the number of hairs per unit area and the average hair thickness are significantly improved, thereby having significantly excellent effects on the treatment of hair loss (experimental examples 4, 5).

The present disclosure provides a composition for preventing hair loss or promoting hair growth comprising a mixture of adenosine, D-panthenol and nicotinamide; and any one or more selected from the group consisting of Polygoni Multiflori radix extract, herba Houttuyniae extract, zinc pyrithione and salicylic acid as effective components. In one aspect of the present disclosure, the present disclosure provides a composition for preventing hair loss or promoting hair growth comprising a mixture of adenosine, D-panthenol and nicotinamide; polygonum multiflorum extract, houttuynia cordata extract or all of them; and zinc pyrithione, salicylic acid or all thereof as an active ingredient. The above composition for preventing hair loss or promoting hair growth produces a remarkable synergistic effect in preventing hair loss or promoting hair growth by combining the above active ingredients.

In one aspect of the present disclosure, the above-mentioned polygonum multiflorum extract or houttuynia cordata extract may be extracted by a method well known in the art, which method is not particularly limited, or a commercially available extract may be utilized. In another aspect, the above-mentioned polygonum multiflorum extract or houttuynia cordata extract may use an extract obtained by extracting polygonum multiflorum or houttuynia cordata with water and/or an organic solvent, and the above-mentioned organic solvent may be a polar organic solvent, a nonpolar organic solvent or a mixed solvent thereof. The polar organic solvent may be a lower alcohol having 1 to 5 carbon atoms, ethyl acetate, acetone, or the like, and the nonpolar organic solvent may be ether, chloroform, benzene, hexane, methylene chloride, or the like. For example, the above lower alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol, isopropanol or the like.

In one aspect of the present disclosure, the above polygonum multiflorum extract or houttuynia cordata extract may include a fraction (fraction) of the primary extract extracted with the above extraction solvent by fractionation (fractionation) with extraction solvents having different polarities. For example, the polygonum multiflorum extract or the houttuynia cordata extract may be a fraction which is fractionated again by using a solvent having different polarities such as ether, benzene, hexane, etc. after extraction with an alcohol having 1 to 5 carbon atoms. In the above fractionation, two or more solvents may be used, and each solvent extract may be prepared by sequential use or mixed use according to the polarity of the solvents.

In one aspect of the present disclosure, the extract prepared as described above or the fraction obtained by performing the fractionation process described above may be subjected to filtration or to a concentration or drying process to remove the solvent, and all of the above filtration, concentration and drying may be performed. Specifically, the filtration may be performed by a filter paper or a reduced pressure filter, the concentration may be performed by reduced pressure concentration using a reduced pressure concentrator or the like, and the drying may be performed by a freeze drying method. Further, the extract prepared as described above or the fraction obtained by performing the above fractionation process is purified by various chromatography methods such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, or the like, whereby a further purified fraction can be obtained.

In one aspect of the present disclosure, a mixture comprising adenosine, D-panthenol and nicotinamide contained in a composition for preventing hair loss or promoting hair growth of the present disclosure relative to the total weight of the composition; and the content of any one or more active ingredients selected from the group consisting of polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione and salicylic acid may be 0.0001 to 10% by weight, preferably 0.001 to 7% by weight, more preferably 0.01 to 5% by weight. When the total content of the above-mentioned active ingredients is less than 0.0001% by weight relative to the total weight of the composition, the effect of promoting induction of hair growth is not remarkable, and when it exceeds 10% by weight, the stability of the dosage form is lowered, so that it is not preferable. In the present disclosure, it was confirmed through experiments that the effects of preventing hair loss and promoting hair growth are remarkably excellent when the effective ingredient is in the above content range.

In one aspect of the present disclosure, the mixture of adenosine, D-panthenol and nicotinamide contained as an active ingredient in the composition for preventing hair loss or promoting hair growth of the present disclosure may be contained in an amount of 0.0001 to 10 wt%, preferably 0.001 to 10 wt%, relative to the total weight of the composition. When the content of the mixture of adenosine, D-panthenol and nicotinamide is less than 0.0001 wt% relative to the total weight of the composition, the effect of promoting hair growth is not remarkable, and when it exceeds 10 wt%, the stability of the dosage form is lowered, which is not preferable.

In one aspect of the present disclosure, any one or more selected from the group consisting of polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione and salicylic acid contained as an additional effective ingredient in the composition for preventing hair loss or promoting hair growth of the present disclosure may be contained in an amount of 0.00001 to 10 wt%, preferably 0.0001 to 5 wt%, with respect to the total weight of the composition. The content of any one or more selected from the group consisting of polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione and salicylic acid is not preferable because the synergistic effect on promoting hair growth is not remarkable when the content is less than 0.00001% by weight, and the stability of the dosage form is lowered when the content exceeds 10% by weight, relative to the total weight of the composition.

In one aspect of the present disclosure, the weight ratio of the mixture of adenosine, D-panthenol and nicotinamide described above may be 1:0.3 to 5:0.2 to 8, preferably 1:0.5 to 4:0.3 to 6 (adenosine: D-panthenol: nicotinamide). When the above-mentioned mixture of adenosine, D-panthenol and nicotinamide is combined in the above-mentioned specific weight ratio, the effect of preventing hair loss and promoting hair growth is excellent.

In one aspect of the present disclosure, the composition for preventing hair loss or promoting hair growth of the present disclosure may be used as a pharmaceutical composition, a pharmaceutical external composition, a cosmetic composition, or a health functional food composition. The compositions of the present disclosure may also be formulated in generally any form that is applicable to the skin, but preferably may be formulated in the form of an external skin preparation. The compositions of the present disclosure may be formulated into skin-applicable dosage forms such as liquid, cream, paste, or solid.

A mixture of adenosine, D-panthenol and nicotinamide of the present disclosure; and any one or more selected from the group consisting of polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione and salicylic acid may be contained in the above composition or dosage form as an alopecia preventing agent or hair growth promoter.

When the dosage form of the present disclosure is in a liquid form, as a carrier component, a solvent, a solubilizing agent, an opacifying agent, or the like can be used, and for example, water, ethanol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylglycol oil, glycerin aliphatic ester, polyethylene glycol, or a fatty acid ester of sorbitan, or the like can be used.

When the dosage form of the present disclosure is a cream, or a gel, as a carrier component, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, or the like can be used.

When the dosage form of the present disclosure is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or the like can be used as a carrier ingredient. In particular, in the case of spray formulations, propellants such as chlorofluorocarbons, propane/butane or dimethyl ether and the like may additionally be included.

In addition to the above-described active ingredients, ingredients that are generally used as external agents applicable to the skin may be contained in the composition of the present disclosure. For example, one or more additives selected from the group consisting of water, surfactants, humectants, lower alcohols, chelating agents, bactericides, antioxidants, preservatives, pigments, fragrances, and the like may be additionally contained.

In addition, the present disclosure provides a product for hair or scalp comprising the above composition for preventing hair loss or promoting hair growth. The hair or scalp product may be any one selected from the group consisting of hair tonic, scalp medical agent, scalp detergent, scalp massage agent, scalp care agent, cleansing agent, shampoo, tonic, hair conditioner, hair lotion, gel, hair mask, cream, essence, powder, spray, oil, soap, ointment, hair styling agent, hair dye, and permanent wave agent, but is not limited thereto.

In one aspect of the present disclosure, the above composition for preventing hair loss or promoting hair growth may be prepared in the form of a hair tonic (hair tonic) or hair lotion (hair condition) (preparation examples 1 to 3).

The composition for preventing hair loss or promoting hair growth of the present disclosure may preferably be used in a transdermal administration method directly applied or sprayed to the skin or the like.

In addition, the present disclosure provides a health functional food composition comprising the above composition for preventing hair loss or promoting hair growth. The above health functional food composition may be prepared by a method generally used in the art. In the above preparation, it can be prepared by adding raw materials and components which are generally added in the art. In addition, the formulation of the above-mentioned health functional food may be prepared without limitation as long as it is a formulation recognized as a health functional food. The health functional food of the present disclosure can be prepared in various forms of dosage forms, and has advantages such as having no side effects or the like that may occur when the medicine is taken for a long period of time using the food as a raw material, and is excellent in portability, and thus can be taken as an auxiliary agent for enhancing the effect of preventing hair loss or promoting hair growth.

In addition, the present disclosure provides a method of preventing hair loss or promoting hair growth, comprising the step of administering to a patient (subject) a composition for preventing hair loss or promoting hair growth, the composition comprising a mixture of adenosine, D-panthenol and nicotinamide; and any one or more selected from the group consisting of Polygoni Multiflori radix extract, herba Houttuyniae extract, zinc pyrithione and salicylic acid as effective components. In one aspect of the present disclosure, the above method of preventing hair loss or promoting hair growth may include the step of treating the scalp or hair of a patient with the above composition for preventing hair loss or promoting hair growth.

The term "administering" as used in this disclosure may refer to introducing the composition of this disclosure by any suitable method. For the route of administration of the compositions of the present disclosure, administration may be by any general route as long as the target tissue is reached. Preferably, transdermal administration is possible, with topical application being most preferred. The number of applications for the compositions of the present disclosure may be determined according to prescription, need, or desire.

The amount of the composition for preventing hair loss or promoting hair growth of the present disclosure to be used may be appropriately adjusted according to individual differences in age, degree of lesions, etc. or dosage forms, and it is generally preferable that an appropriate amount is applied to the scalp 1 to several times a day for 1 week to several months. In the experimental examples of the present disclosure, the hair loss treatment composition 1 was used 5 times per week for 6 months, and as a result, it was confirmed that the hair growth promoting effect was excellent (experimental examples 4 and 5).

All ingredients described in the present disclosure preferably do not exceed the maximum use value specified in the relevant regulations, specifications (for example, relevant regulations such as cosmetic safety standards (korea), cosmetic safety technical specifications (china), food act (korea), food additive act (korea), health function food act (korea)) and the like of korea, china, united states, europe, japan and the like. That is, preferably, the pharmaceutical composition for external use, the cosmetic composition or the health functional food composition of the present disclosure contains the components of the present disclosure at the content limits allowed in the relevant regulations and specifications of each country.

Effects of the invention

The composition for preventing hair loss and promoting hair growth of the present disclosure promotes activity of papilla cells, mitochondrial activity of papilla cells and activity of Wnt/β -catenin signaling system through a combination between specific active ingredients, thereby producing a remarkable synergistic effect on the effects of preventing hair loss and promoting hair growth.

Detailed Description

In the following, examples and the like will be described in detail to aid in understanding the present invention. However, the embodiments of the present invention may be modified in various different forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the present invention are provided to more fully explain the present invention to those skilled in the art to which the present invention pertains.

Preparation example 1: composition 1 for treating alopecia (hair tonic)

A hair tonic was prepared by a conventional method according to the formula disclosed in Table 1 below, varying the weight ratio of the mixture of adenosine, D-panthenol, nicotinamide.

TABLE 1

Preparation example 2: composition 2 for treating alopecia (hair tonic)

According to the prescription disclosed in the following Table 2, each of Polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione or salicylic acid is added, respectively, and a hair tonic is prepared by a conventional method.

TABLE 2

Preparation example 3: composition 3 for treating alopecia (Hair dew)

Adding one or more of Polygoni Multiflori radix extract, herba Houttuyniae extract, zinc pyrithione, and salicylic acid into mixture of adenosine, D-panthenol and nicotinamide according to the formula disclosed in table 3, and making into hair cream by conventional method.

TABLE 3

Experimental example 1: effect of enhancing activity of papilla cells

Human hair papilla cells (dermal papilla cells, dermal papilla cells, DPCs) were purchased from PromoCell company. The DPCs were treated with 5% CO at 37℃in DMEM (Hyclone Inc, UT, USA) containing 5% fetal bovine serum (FBS; gibco, N.Y., USA), penicillin 100unit/mL and streptomycin 100. Mu.g/mL ₂ Culturing under the condition. The cultured DPC s 3,000 cells/well were plated in 96-well plates and then cultured under 0.1% serum conditions for 24 hours. Thereafter, in cultured cells, the cells will be treated with serum-free DMEMIn (1:1000) diluted DMSO (vehicle) as control group, minoxidil 2 μg/ml as positive control group, and one or more of Polygoni Multiflori radix extract, herba Houttuyniae extract, zinc pyrithione, salicylic acid in a mixture of adenosine, D-panthenol, nicotinamide were treated for 1 day and incubated (incubations) as shown in table 4 below. After incubation, the degree of enhancement of the activity of the cells was evaluated using the CCK method. CCK-8 was treated at 1:10 in culture and incubated for 1 hour. After 1 hour, the absorbance (absorptance) of each well (well) was measured at 450 nm. All experiments were repeated 3 times and the average of absorbance values was calculated. For the above results, the control group was set to 100, which is expressed as a percentage of the treatment group relative to the control group.

TABLE 4

From the results disclosed in table 4 above, it was confirmed that when one or more of polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione, salicylic acid was further contained in the mixture of adenosine, D-panthenol and nicotinamide and treated, a remarkable synergistic effect was produced in enhancing the activity of hair papilla cells.

Experimental example 2: promoting effect of mitochondrial Activity of papilla cells

Human hair papilla cells (dermal papilla cells, dermal papilla cells, DPCs) were purchased from PromoCell company. The DPCs were treated with 5% CO at 37℃in DMEM (Hyclone Inc, UT, USA) containing 5% fetal bovine serum (FBS; gibco, N.Y., USA), penicillin 100unit/mL and streptomycin 100. Mu.g/mL ₂ Culturing under the condition. The cultured DPC s 3,000 cells/well were plated in 96-well plates and then cultured under 0.1% serum conditions for 24 hours. Thereafter, in cultured cells, DMSO (vehicle) diluted at 1:1000 in serum-free DMEM was used as a control group, minoxidil 2 μg/ml was used as a positive control group, and one or more of Polygoni Multiflori radix extract, herba Houttuyniae extract, zinc pyrithione, salicylic acid in a mixture of adenosine, D-panthenol, nicotinamide was treated for 1 day as shown in Table 5 belowAnd incubation was performed. After incubation, the extent of proliferation of cells was assessed using the JC-1 method. JC-1 was treated at 1:100 in culture and incubated for 1 hour. After 1 hour, fluorescence (fluorescence) was measured for each well at 590nm and 530 nm. All experiments were repeated 3 times and the mean of the fluorescence values was calculated. For the above results, the control group was set to 100, which is expressed as a percentage of the treatment group relative to the control group.

TABLE 5

From the results disclosed in table 5 above, it was confirmed that when one or more of polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione, salicylic acid was further contained in the mixture of adenosine, D-panthenol and nicotinamide and treated, a remarkable synergistic effect was produced in enhancing mitochondrial activity of hair papilla cells.

Experimental example 3: modulation of hair papilla cell Activity Signal (Wnt/beta-catenin)

In general, wnt/β -catenin signaling activated in the hair papilla occurs when the growth cycle of the hair transitions from the catagen phase to the active phase, i.e., during the entire active phase from the beginning of hair growth to the occurrence of hair growth. In telogen and catagen phases, wnt/β -catenin signaling decreases or disappears, and hair follicle degeneration and hair loss occur. Thus, it was confirmed by this experiment how much one or more treatment groups of polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione, salicylic acid in the mixture of adenosine, D-panthenol, nicotinamide of the present disclosure contributed to Wnt/β -catenin signal amplification.

Wnt3a protein was used as a positive control group for amplifying Wnt/β -catenin signal, and DMSO was used as a negative control group. Wnt Reporter HEK293A cells 3X10 in culture 96-well plates ⁴ Inoculating into each well, and then treating Polygoni Multiflori radix extract, herba Houttuyniae extract, zinc pyrithione, and salicylic acid in the mixture of adenosine, D-panthenol, and nicotinamide of the present disclosure as shown in Table 6 belowMore than one kind of acid. Then, report analysis (reporter assay) was performed using a luciferase assay kit (Luciferase assay kit) from Promega corporation (E1960). The Wnt/β -catenin promoter activity was measured according to the experimental method of manufacturing company and using photometer Victor (luminometer Victor) (perkin elmer, walthamm, ma, USA).

TABLE 6

From the results disclosed in table 6 above, it was confirmed that when one or more of polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione, salicylic acid was further contained in the mixture of adenosine, D-panthenol and nicotinamide and treated, the activity of Wnt/β -catenin signaling system was synergistically enhanced. Since Wnt/β -catenin signaling is a hair growth promoting signaling of hair follicle stem cells, it can be seen from the above experimental results that the composition of the present disclosure exhibits a remarkable ascending effect in promoting hair growth.

Experimental example 4: hair tonic composition 1 (hair tonic) for treating alopecia or promoting hair growth

The hair growth effect of the composition 1 (hair tonic) for treating hair loss or promoting hair growth of the present invention was tested on a total of 70 men and women whose hair number is significantly smaller than that of normal or weaker hair with hair loss symptoms. The total of 70 men and women were divided into 7 groups of 10, and the hair and scalp were applied for 6 months 5 times per week using comparative examples 1, 2 and examples 1 to 5. After use, a study evaluation by clinical photograph and an evaluation by hair photographic image (photo-mask) were performed. The study evaluation by clinical photograph was carried out 2 or 6 months after coating, and the comparative examples and examples were evaluated in 3 items of "good", "slight" or "no change" (good: improvement by 50% to 75%, slight (slight): improvement by 25% to 50%, no change): no change). Evaluation of the number of hairs per unit area and the average hair thickness by the hair image was performed after 6 months of application, and the results of the measurements for the comparative examples and examples are shown in table 7.

TABLE 7

From the results disclosed in table 7 above, it was confirmed that when a mixture comprising adenosine, D-panthenol and nicotinamide was treated, the number of hairs per unit area and the average hair thickness were increased. In particular, it was confirmed that the number of hairs per unit area and the average hair thickness-increasing effect were significantly enhanced when adenosine, D-panthenol and nicotinamide were mixed and treated in a specific weight ratio according to the present disclosure.

Experimental example 5: hair tonic composition 2 (hair tonic) for treating alopecia or promoting hair growth

The hair growth effect of the composition 2 (hair tonic) for treating hair loss or promoting hair growth of the present invention was tested on a total of 60 men and women whose hair number is significantly smaller than that of normal or weaker hair with hair loss symptoms. The total of 60 men and women were divided into 6 groups by 10, and the hair and scalp were applied for 6 months 5 times per week using comparative example 1 and examples 6 to 9. After use, a study evaluation by clinical photograph and an evaluation by hair photographic image (photo-mask) were performed. The study evaluation by clinical photograph was carried out 2 or 6 months after coating, and the comparative examples and examples were evaluated in 3 items of "good", "slight" or "no change" (good: improvement by 50% to 75%, slight (slight): improvement by 25% to 50%, no change): no change). Evaluation of the number of hairs per unit area and the average hair thickness by the hair image was performed after 6 months of application, and the results of the measurements for the comparative examples and examples are shown in table 8.

TABLE 8

From the results disclosed in table 8 above, it was confirmed that when one or more of polygonum multiflorum extract, houttuynia cordata extract, zinc pyrithione, salicylic acid was further contained in the mixture of adenosine, D-panthenol and nicotinamide and treated, the number of hairs per unit area and the average hair thickness were significantly increased as compared with examples 1 to 5, thereby exhibiting significant synergistic effects in preventing hair loss and promoting hair growth, as shown in examples 6 to 9.

The above-described formulation examples of the hair tonic or hair lotion are only one example of the composition for preventing hair loss or promoting hair growth of the present disclosure, and it is apparent to those of ordinary skill in the art that the scope of the composition of the present disclosure is not limited to only these formulations.

Claims

1. A composition for preventing hair loss or promoting hair growth comprises

A mixture of adenosine, D-panthenol and nicotinamide; and

any one or more selected from the group consisting of Polygoni Multiflori radix extract, herba Houttuyniae extract, zinc pyrithione and salicylic acid as effective components.

2. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the content of the above active ingredient is 0.0001 to 10% by weight relative to the total weight of the composition.

3. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition enhances activity of hair papilla cells.

4. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition promotes mitochondrial activity of papilla cells.

5. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition promotes activity of Wnt/β -catenin signaling system.

6. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition is a pharmaceutical composition.

7. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition is a pharmaceutical external composition.

8. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition is a cosmetic composition.

9. A hair or scalp product comprising the composition for preventing hair loss or promoting hair growth of claim 1.

10. A hair or scalp product according to claim 9 wherein said product is selected from the group consisting of hair tonic, scalp treatment, scalp detergent, scalp massage, scalp care, cleansing, shampoo, tonic, hair conditioner, hair lotion, gel, hair film, cream, essence, powder, spray, oil, soap, ointment, hair styling agent, hair dye and permanent waving agent.

11. A composition for preventing hair loss or promoting hair growth, characterized in that, in a composition for preventing hair loss or promoting hair growth comprising a mixture of adenosine, D-panthenol and nicotinamide as an active ingredient,

the weight ratio of the mixture is 1:0.3-5:0.2-8 (adenosine: D-panthenol: nicotinamide).