KR20220008790A - Composition for preventing hair loss and promoting hair growth - Google Patents

Abstract

The present disclosure relates to a composition for preventing hair loss and promoting hair growth, containing an ingredient which induces hair growth promotion. The composition for preventing hair loss and promoting hair growth of the present disclosure promotes the activity of dermal papilla cells, the mitochondrial activity of dermal papilla cells, and the activity of a Wnt/beta-catenin signaling system through the combination of specific active ingredients, thereby producing a remarkable synergistic effect on preventing hair loss and promoting hair growth.

Description

Composition for preventing hair loss and promoting hair growth

The present invention relates to a composition for preventing hair loss and promoting hair growth.

In the process of increasing interest in beauty as the times change, hair occupies an important part. Hair is a thin, keratinized structure produced on the surface of the skin. In addition to the role of cushion against external shock, it protects the human body from external stimuli such as direct sunlight, cold, friction, and danger, and discharges heavy metals such as arsenic, mercury and zinc that are harmful to the body. Aspects are also highlighted. However, an increasing number of people complain of hair loss due to various causes, including changes in diet and increased internal and external stress. It is known that genetic factors act as the main cause of hair loss, but as mentioned above, increase in social stress, environmental pollution, frequent perms and dyeing, and incorrect scalp care are also emerging as causes of hair loss.

Human hair has about 100,000 to 150,000 hairs, and each hair has a different growth cycle. In early development of hair follicles, placode is formed by basal epidermal cells by the action of beta-catenin (Sarah E. Miller, 2002). Dermal fibroblasts receiving a signal from the formed placode form a dermal condensate. At the same time, the placode grows and infiltrates in the dermis direction and merges with the skin condensate to form a primary hair germ. The epidermal cells that form the hair embryo grow long in the direction of the dermis, and form a hair peg, a long columnar structure made of multiple layers, while the skin condensate is formed into a spherical dermal papilla. do. The tip of the hair peg becomes thicker to form a hair bulb, which wraps around the dermal papilla to complete the hair follicle, and the epidermal cells inside the hair follicle differentiate to produce hair.

The first generated hair goes through a growth phase, a degeneration phase, and a resting phase, and then goes through a cycle of falling out and entering the growth phase again. The hair growth cycle consists of three phases: the anagen stage, in which hair is most actively grown, the catagen stage, in which hair degeneration begins, and the telogen stage, in which hair growth stops or enters rest. ) is classified as

In regulating the hair growth cycle, the activity of outer root sheath cells including dermal papilla cells and hair follicle stem cells constituting hair follicles, and various cytokines and growth factors produced in these cells play an important role is known to do For example, DKK-1 (Dickkopf-1) plays an important role in hair follicle growth inhibition and death. On the other hand, for periodic hair growth, regeneration of hair follicles and activation of stem cells are required, which is known to be accomplished by the generation and activation of Wnt/β-catenin. In addition, growth factors such as VEGF, IGF-1, and FGF are known to promote the growth phase of hair, and TGFb-1 and EGF are known as growth factors that induce the degenerative phase of hair follicles.

In general, alopecia refers to an increase in the number of abnormal hair loss due to a shorter hair growth phase and more hair in a degenerative or resting phase among these cycles. Hair loss is a normal phenomenon, but a normal person has a lot of hair in a growth phase, whereas a person with normal alopecia has a lot of hair in a telogen state, so visible hair loss is shown. A characteristic of people with alopecia is the miniaturization of their hair. As hair loss progresses, the growth period becomes shorter and the transition to the catagen and resting phases is accelerated, and the volume of the dermal papilla that follows becomes smaller and the hair follicles become smaller. Therefore, for the treatment of hair loss, it is important to allow the hair follicles in the resting state to quickly transition to the growth phase and to extend the shortened growth phase.

As mentioned above, the causes of hair loss include male hormone action, excessive sebum secretion, poor blood circulation, decreased scalp function due to peroxide or bacteria, genetic factors, aging, and stress have been discussed. However, until now, the clear cause of hair loss has not been elucidated, and it is known that very complex actions are intertwined.

For these various and complex causes of hair loss, the currently known hair loss prevention products have active ingredients that promote blood circulation, strengthen hair root function, moisturize the scalp, prevent dandruff, have an antioxidant effect, extend the hair growth phase, and suppress the action of male hormones. Although the target ingredient is included, there is still no one with a clear effect in terms of effect, and the problem of side effects is raised.

On the other hand, adenosine is a component of amino acids made in cells and is a main component of cellular energy metabolism. Adenosine is one of the constituent elements of DNA, which is a gene, and is a compound in which a base called adenine and a sugar called ribose are combined. Adenosine becomes ATP (adenosine 5'-triphosphate), which is the main source of intracellular energy when three phosphates are combined with sugar. Accordingly, adenosine is known as an important factor that plays a decisive role in all metabolism smoothly proceeding while composing the body. Because adenosine is an intracellular component, it has excellent stability and durability when penetrating into the skin, and has a synergistic effect on skin wrinkles by promoting DNA and protein synthesis in the dermal layer.

In addition, niacinamide is a kind of vitamin B complex, coexisting with nicotinic acid and widely distributed in the plant world, and exists as a component of the redox coenzyme NAD or NADP in the living body of the animal system. Niacinamide is a bioactive amide of vitamin B3, also known as pyridine-3-carboxylic acid, which is also known to act as an anti-inflammatory raw material or antioxidant. Niacinamide inhibits the movement of melanosomes, thereby inhibiting the movement of synthesized melanin to keratinocytes, thereby reducing skin pigmentation and enhancing whitening action. In addition, niacinamide increases the synthesis of collagen and intercellular lipids, thereby having an advantageous effect on skin moisturizing and the like.

In addition, D-panthenol (dexpanthenol) can be called provitamin B5 in the alcohol form of vitamin B5 (Pantothenic acid). D-panthenol is absorbed into the skin and converted directly to vitamin B5 for use. D-panthenol is a component of a skin moisturizer and wrinkle improvement agent, and is widely used in cosmetics and household products, and is also used as an ointment for wound healing, skin burns, and itchiness improvement. In addition, D-panthenol is known as a permeable moisturizer, and it is known for its effects of conditioning the skin and hair and increasing the recovery of damaged hair.

In addition, succulents are perennial herbaceous medicinal plants belonging to the family Knobaceae, and their scientific names are Pleuropterus multiflorus , Polygonum multiflorum , Fagopyrum multiflorum , Reynoutria multiflora , or Fallopia multiflora . Sauerkraut extract is used as a tonic, stimulant, analgesic, antihypertensive, or antitussive, and is known to be used to treat colds, hematemesis, nervous breakdown, or arthritis.

In addition, Eoseongcho ( Houttuynia cordata ) is a medicinal herb known as Yakmomil, Chinese medicinal herb, Jueupchae or Sipyak as a perennial plant of the family Trifolium . Eoseongcho is called eoseongcho because it contains various essential oils and gives off a unique smell like fishy smell. Eoseongcho blooms mainly from May to July, and is a medicinal herb used in folk medicine for gonorrhea, enteritis, urinary tract infection, pneumonia, or bronchitis.

In addition, zinc pyrithione has an antibacterial action, preventing the growth of microorganisms on the scalp and skin. In particular, zinc pyrithione has a proliferation inhibitory effect on malassezia and other fungi, and serves to prevent seborrheic dermatitis and dandruff on the scalp.

In addition, salicylic acid is the main component of aspirin and has been widely used as an anti-inflammatory, analgesic or antipyretic agent. Salicylic acid exfoliates and softens the skin. Salicylic acid is mainly added to cosmetics such as cleansing foams to soften and exfoliate dead skin cells to soften pores. Through this, salicylic acid can help discharge sebum in the pores and keep the skin clean. In addition, salicylic acid exhibits antibacterial and anti-inflammatory action, thereby inhibiting the growth of the scalp or skin microorganisms, thereby improving the skin and scalp or preventing dandruff.

Meanwhile, minoxidil (2,4-diamino-6-piperidinopyrimidine-3-oxide) is the only hair loss treatment approved by the US Food and Drug Administration (FDA) at present, but when used for 32 to 48 weeks, the effect is 38% each. and 13%, which is unsatisfactory, and has a disadvantage in that the effect is inferior to the bangs.

As such, there is a need for the development of a composition excellent in the effect of preventing hair loss or promoting hair growth. There is a lack of research on the effect of promoting hair growth.

The problem to be solved by the present disclosure is to provide a composition for preventing hair loss or promoting hair growth having an excellent hair loss prevention and/or hair growth promoting effect.

In addition, the problem to be solved by the present disclosure is to provide a product for hair or scalp comprising the composition for preventing hair loss or promoting hair growth.

The present inventors improve the problems such as side effects and precautions for use of conventional hair loss treatment agents, and without disadvantages such as weak hair growth promoting effect of hair loss prevention / hair growth agent, it is safe for the human body and can effectively act in preventing hair loss and promoting hair growth Efforts were made to develop a composition that has As a result, the present inventors have found that when a specific active ingredient is further combined with a mixture of adenosine, D-panthenol and niacinamide, a remarkable synergistic effect occurs in preventing hair loss and promoting hair growth.

Specifically, the present inventors added any one or more selected from the group consisting of adenosine, D-panthenol and niacinamide to a mixture of adenosine, D-panthenol and niacinamide through an in-vitro experiment When combined, there is a significant synergistic effect on the dermal papilla cell activity enhancing effect (Experimental Example 1), the dermal papilla cell mitochondrial activity promoting effect (Experimental Example 2), and the dermal papilla cell activation signal (Wnt/β-catenin) promoting effect (Experimental Example 3) was confirmed to have occurred. In addition, the present inventors confirmed that, when treating alopecia patients with a composition for preventing hair loss or promoting hair growth comprising the above combination, the number of hairs per unit area and average hair thickness were remarkably improved to have a remarkably excellent effect in the treatment of alopecia (Experimental Examples 4 and 5).

The present disclosure relates to a mixture of adenosine, D-panthenol and niacinamide; And it provides a composition for preventing hair loss or promoting hair growth, comprising as an active ingredient any one or more selected from the group consisting of sauerkraut extract, eosincho extract, zinc pyrithione and salicylic acid. In one aspect of the present disclosure, the present disclosure provides a mixture of adenosine, D-panthenol and niacinamide; Sauerkraut extract, Eosinosaurus extract, or both; and zinc pyrithione, salicylic acid, or both as an active ingredient. It provides a composition for preventing hair loss or promoting hair growth. The composition for preventing hair loss or promoting hair growth has a remarkable synergistic effect in preventing hair loss or promoting hair growth by the combination between the active ingredients.

In one aspect of the present disclosure, the sewage extract or Eoseongcho extract may be extracted by a method known in the art, the method is not particularly limited, or a commercially available extract may be used. In another aspect, the sewage extract or eoseongcho extract may be an extract obtained by extracting sewage or eoseongcho with water and/or an organic solvent, and the organic solvent may be a polar organic solvent, a non-polar organic solvent, or a mixed solvent thereof. have. The polar organic solvent may be a lower alcohol having 1 to 5 carbon atoms, ethyl acetate or acetone, and the non-polar organic solvent may be ether, chloroform, benzene, nucleic acid or dichloromethane. For example, the lower alcohol having 1 to 5 carbon atoms may be methanol, ethanol, propanol, butanol or isopropanol.

In one aspect of the present disclosure, the sewage extract or Eoseongcho extract may include a fraction obtained by fractionating the primary extract extracted using the above-described extraction solvent using an extraction solvent having a different polarity. For example, the sewage extract or eosincho extract may be a fraction obtained by extracting with an alcohol having 1 to 5 carbon atoms, and then re-fractioning with a solvent having a different polarity, such as ether, benzene, or hexane. In the fractionation, two or more solvents may be used, and each solvent extract may be prepared by sequentially using or mixing them according to the polarity of the solvent.

In one aspect of the present disclosure, the solvent may be removed by filtering or performing a concentration or drying process on the prepared extract or the fraction obtained by performing the fractionation process, and all of the filtration, concentration and drying may be performed. have. Specifically, the filtration may be performed using filter paper or a reduced pressure filter, and the concentration may be concentrated under reduced pressure using a reduced pressure concentrator or the like, and drying may be performed by a freeze-drying method. In addition, further purified fractions can be obtained by purifying the prepared extract or the fraction obtained by performing the fractionation process using various chromatography methods such as silica gel column chromatography, thin layer chromatography, or high performance liquid chromatography. .

In one aspect of the present disclosure, a mixture of adenosine, D-panthenol and niacinamide included in the composition for preventing hair loss or promoting hair growth of the present disclosure; And the content of the active ingredient comprising any one or more selected from the group consisting of sauerkraut extract, eosincho extract, zinc pyrithione and salicylic acid is 0.0001 to 10% by weight, preferably 0.001 to 7% by weight, more preferably from the total weight of the composition. It may be 0.01 to 5% by weight. When the total content of the active ingredients is less than 0.0001% by weight relative to the total weight of the composition, the effect of promoting hair growth is insignificant, and when it exceeds 10% by weight, the stability of the formulation is deteriorated, which is not preferable. In the present disclosure, it was confirmed through an experiment that the effect of preventing hair loss and promoting hair growth was remarkably excellent when the active ingredient was in the above content range.

In one aspect of the present disclosure, the content of the mixture of adenosine, D-panthenol and niacinamide included as an active ingredient in the composition for preventing hair loss or promoting hair growth of the present disclosure is 0.0001 to 10% by weight, preferably 0.001 to 10% by weight relative to the total weight of the composition. 10% by weight. When the content of the mixture of adenosine, D-panthenol, and niacinamide is less than 0.0001% by weight relative to the total weight of the composition, the effect of promoting hair growth is insignificant, and when it exceeds 10% by weight, the stability of the formulation is deteriorated, which is not preferable.

In one aspect of the present disclosure, the content of any one or more selected from the group consisting of Sauerkraut extract, Echo extract, zinc pyrithione and salicylic acid included as an additional active ingredient in the composition for preventing hair loss or promoting hair growth of the present disclosure is based on the total weight of the composition. 0.00001 to 10% by weight, preferably 0.0001 to 5% by weight. When the content of any one or more selected from the group consisting of the sauerkraut extract, eosinh extract, zinc pyrithione and salicylic acid is less than 0.00001% by weight relative to the total weight of the composition, the synergistic effect in promoting hair growth is insignificant, and when it exceeds 10% by weight, the formulation It is undesirable because of its poor stability.

In one aspect of the present disclosure, the weight ratio of the mixture of adenosine, D-panthenol and niacinamide is 1:0.3-5:0.2-8, preferably 1:0.5-4:0.3-6 (adenosine:D-panthenol:niacin) amide). When the mixture of adenosine, D-panthenol and niacinamide is combined in the specific weight ratio, the effect of preventing hair loss and promoting hair growth is excellent.

In one aspect of the present disclosure, the composition for preventing hair loss or promoting hair growth of the present disclosure may be used as a pharmaceutical composition, a composition for quasi-drugs, a cosmetic composition, or a health functional food composition. The composition of the present disclosure may be formulated in any form that can be applied to the skin, but may preferably be formulated in the form of an external preparation for skin. The composition of the present disclosure may be prepared in a formulation that can be applied to the skin, such as liquid, cream, paste, or solid, for example.

a mixture of adenosine, D-panthenol and niacinamide of the present disclosure; And any one or more selected from the group consisting of sauerkraut extract, eoseongcho extract, zinc pyrithione and salicylic acid may be included in the composition or formulation as an anti-hair loss agent or a hair growth promoter.

When the formulation of the present disclosure is liquid, a solvent, solubilizer or emulsifier may be used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

When the formulation of the present disclosure is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. have.

When the formulation of the present disclosure is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder, etc. may be used as a carrier component. In particular, in the case of a spray formulation, a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether may be additionally included.

The composition of the present disclosure may include ingredients commonly used as external preparations that can be applied to the skin in addition to the active ingredients. For example, one or more additives selected from the group consisting of water, surfactants, humectants, lower alcohols, chelating agents, bactericides, antioxidants, preservatives, colorants and fragrances, etc. may be further included.

In addition, the present disclosure provides a product for hair or scalp comprising the composition for preventing hair loss or promoting hair growth. The hair or scalp products include hair growth agents, scalp clinic agents, scalp scaling agents, scalp massage agents, scalp care agents, cleaners, shampoos, tonics, hair conditioners, hair lotions, gels, packs, creams, essences, powders, sprays, oils , but may be any one selected from the group consisting of soap, ointment, hair styling agent, hair dye and perm agent, but is not limited thereto.

In one aspect of the present disclosure, the composition for preventing hair loss or promoting hair growth may be prepared in the form of a hair tonic or hair lotion (Preparation Examples 1 to 3).

The composition for preventing hair loss or promoting hair growth of the present disclosure can be preferably used in a transdermal administration method such as directly applied to the skin or sprayed.

In addition, the present disclosure provides a health functional food composition comprising the composition for preventing hair loss or promoting hair growth. The health functional food composition may be prepared by a method commonly used in the art. In the preparation, it can be prepared by adding raw materials and components commonly added in the art. In addition, the dosage form of the health functional food may be manufactured without limitation as long as it is a dosage form recognized as a health functional food. The health functional food of the present disclosure can be prepared in various types of dosage forms, and has the advantage of not having side effects that may occur during long-term administration of drugs using food as a raw material, and has excellent portability to prevent hair loss or promote hair growth. It can be taken as a supplement for

Also disclosed herein is a mixture of adenosine, D-panthenol and niacinamide; And anti-hair loss or hair growth comprising administering to a patient a composition for preventing or promoting hair growth comprising, as an active ingredient, any one or more selected from the group consisting of sauerkraut extract, Echo extract, zinc pyrithione and salicylic acid It provides a method of promotion. In one aspect of the present disclosure, the method for preventing hair loss or promoting hair growth may include treating the composition for preventing hair loss or promoting hair growth on the scalp or hair of a patient.

As used herein, the term “administration” may refer to introducing a composition of the present disclosure by any suitable method. The administration route of the composition of the present disclosure may be administered through any general route as long as it can reach the target tissue. Preferably, it can be administered transdermally, and among them, topical application is most preferable. The number of times of application of the composition of the present disclosure may be determined according to the prescription, need or desire.

The amount of the composition for preventing hair loss or promoting hair growth of the present disclosure can be appropriately adjusted according to individual differences or formulations such as age, the degree of lesion, etc., and usually an appropriate amount is applied to the scalp once to several times a day and used for one week to several months. It is preferable to do In the experimental example of the present disclosure, composition 1 for the treatment of hair loss was used 5 times a week for 6 months, and as a result, it was confirmed that an excellent hair growth promoting effect appeared (Experimental Examples 4 and 5).

All ingredients described in the present disclosure, preferably, relevant laws and regulations of Korea, China, the United States, Europe, Japan, etc. (eg, regulations on cosmetic safety standards (Korea), cosmetic safety technical standards (China), Do not exceed the maximum use value prescribed by the Food and Additives Code (Korea), Food Additives Code (Korea), and Health Functional Food Code (Korea)). That is, preferably, the pharmaceutical composition, the quasi-drug composition, the cosmetic composition, or the health functional food composition according to the present disclosure includes the components according to the present disclosure within the content limit allowed by the relevant laws and regulations of each country.

The composition for preventing hair loss and promoting hair growth of the present disclosure promotes the activity of dermal papilla cells, mitochondrial activity of dermal papilla cells, and Wnt/β-catenin signaling system through a combination between specific active ingredients, thereby having a significant effect in preventing hair loss and promoting hair growth create a synergistic effect.

Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art to which the present invention pertains.

Preparation Example 1: Composition 1 for the treatment of hair loss (Hair Tonic)

By varying the weight ratio of the mixture of adenosine, D-panthenol, and niacinamide, a hair tonic was prepared in a conventional manner according to the prescription disclosed in Table 1 below.

Ingredients ethanol castor oil glycerin adenosine D-Panthenol Niacinamide fragrances and colors Purified water Weight ratio (%) Comparative Example 1 55 5 3 0 0 0 appropriate amount remaining amount Comparative Example 2 55 5 3 One One One appropriate amount Example 1 55 5 3 1.6 0.9 0.5 appropriate amount Example 2 55 5 3 0.8 1.5 0.7 appropriate amount Example 3 55 5 3 0.75 0.75 1.5 appropriate amount Example 4 55 5 3 0.6 0.8 1.6 appropriate amount Example 5 55 5 3 0.3 One 1.7 appropriate amount

Preparation Example 2: Composition 2 for the treatment of hair loss (Hair Tonic)

According to the prescription disclosed in Table 2 below, a hair tonic was prepared in a conventional manner by adding one each of a sauerkraut extract, eoseongcho extract, zinc pyrithione, or salicylic acid.

Ingredients ethanol castor oil glycerin adenosine D-Panthenol Niacinamide sewage extract Eoseongcho Extract zinc pyrithione salicylic acid fragrances and colors Purified water Weight ratio (%) Example 6 55 5 3 0.4 0.6 1.0 One appropriate amount remaining amount Example 7 55 5 3 0.4 0.6 1.0 One appropriate amount Example 8 55 5 3 0.4 0.6 1.0 One appropriate amount Example 9 55 5 3 0.4 0.6 1.0 One appropriate amount

Preparation Example 3: Composition 3 for the treatment of hair loss (hair lotion)

In a mixture of adenosine, D-panthenol, and niacinamide, at least one of Sauerkraut extract, Echo extract, zinc pyrithione, and salicylic acid was prepared in a conventional manner according to the prescription disclosed in Table 3 below.

Ingredients Weight ratio (%) cetostearyl alcohol 2 Stearyltriethylammonium chloride 2 Hydroxyethyl Cellulose 0.5 adenosine One D-Panthenol 1.5 Niacinamide 3 One or more of Sewage extract, Eosinaceae extract, zinc pyrithione, and salicylic acid One fragrances and colors appropriate amount Purified water Remaining amount (total 100)

Experimental Example 1: Effect of promoting dermal papilla cell activity

Human-derived dermal papilla cells (DPCs) were purchased from Promocell. The DPCs were incubated in DMEM (Hyclone Inc, UT, USA) containing 5% fetal bovine serum (FBS; Gibco, NY, USA), penicillin 100 unit/mL and streptomycin 100 μg/mL at 37° C. and 5% CO ₂ cultured under conditions. After dispensing the cultured DPCs 3,000 cells/well in a 96-well plate, they were cultured for 24 h in 0.1% serum condition. Then, DMSO (vehicle) diluted 1:1000 in serum-free DMEM in the cultured cells as a control group, minoxidil 2 μg/ml as a positive control group, adenosine, D-panthenol, and niacinamide in a mixture of S. Extract, zinc pyrithione, and at least one of salicylic acid as shown in Table 4 below Treated and incubated for 1 day. After incubation, the degree of cell activity enhancement was evaluated using the CCK method. CCK-8 was treated at 1:10 in the culture medium and incubated for 1 hour. After 1 hour, the absorbance of each well was measured at 450 nm. All experiments were repeated three times and the average of the absorbance values was calculated. The results were expressed as a percentage of the treatment group compared to the control group as 100.

treatment group dermal papilla cell activation capacity (%) untreated group 100 Adenosine (0.06%) + D-panthenol (0.08%) + Niacinamide (0.16%) 149.7 Adenosine (0.04%)+D-panthenol (0.06%)+niacinamide (0.1%)+Sewage extract (0.1%) 168.6 Adenosine (0.04%) + D-panthenol (0.06%) + niacinamide (0.1%) + Echo extract (0.1%) 169.4 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Zinc pyrithione (0.1%) 160.3 Adenosine (0.04%)+D-panthenol (0.06%)+niacinamide (0.1%)+salicylic acid (0.1%) 166.3 Adenosine (0.04%)+D-panthenol (0.06%)+niacinamide (0.1%)+Sewage extract (0.05%)+Zinc pyrithione (0.05%) 177.4 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Sewage extract (0.05%) + Salicylic acid (0.05%) 178.0 Adenosine (0.04%) + D-panthenol (0.06%) + niacinamide (0.1%) + Echo extract (0.05%) + zinc pyrithione (0.05%) 183.7 Adenosine (0.04%) + D-panthenol (0.06%) + niacinamide (0.1%) + Echo extract (0.05%) + salicylic acid (0.05%) 187.7

When looking at the results disclosed in Table 4 above, when treating with a mixture of adenosine, D-panthenol, and niacinamide, one or more of Sauerkraut extract, Echo extract, zinc pyrithione, and salicylic acid is additionally included, dermal papilla cell activation ability It was confirmed that a significant synergistic effect occurred in the enhancement.

Experimental Example 2: Effect of promoting dermal papilla cell mitochondrial activity

Human-derived dermal papilla cells (DPCs) were purchased from Promocell. The DPCs were incubated in DMEM (Hyclone Inc, UT, USA) containing 5% fetal bovine serum (FBS; Gibco, NY, USA), penicillin 100 unit/mL and streptomycin 100 μg/mL at 37° C. and 5% CO ₂ cultured under conditions. After dispensing the cultured DPCs 3,000 cells/well in a 96-well plate, they were cultured for 24 h in 0.1% serum condition. Then, DMSO (vehicle) diluted 1:1000 in serum-free DMEM in the cultured cells as a control group, minoxidil 2 μg/ml as a positive control group, adenosine, D-panthenol, and niacinamide in a mixture of S. Extract, zinc pyrithione, and at least one of salicylic acid as shown in Table 5 below Treated and incubated for 1 day. After incubation, the degree of cell proliferation was evaluated using the JC-1 method. JC-1 was treated at 1:100 in the culture medium and incubated for 1 hour. After 1 hour, the fluorescence of each well was measured at 590 nm and 530 nm. All experiments were repeated three times and the average of the fluorescence values was calculated. The results were expressed as a percentage of the treatment group compared to the control group as 100.

treatment group dermal papilla cell mitochondrial activity (%) untreated group 100 Adenosine (0.06%) + D-panthenol (0.08%) + Niacinamide (0.16%) 160.5 Adenosine (0.04%)+D-panthenol (0.06%)+niacinamide (0.1%)+Sewage extract (0.1%) 169.4 Adenosine (0.04%) + D-panthenol (0.06%) + niacinamide (0.1%) + Echo extract (0.1%) 177.5 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Zinc pyrithione (0.1%) 173.6 Adenosine (0.04%)+D-panthenol (0.06%)+niacinamide (0.1%)+salicylic acid (0.1%) 179.3 Adenosine (0.04%)+D-panthenol (0.06%)+niacinamide (0.1%)+Sewage extract (0.05%)+Zinc pyrithione (0.05%) 188.3 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Sewage extract (0.05%) + Salicylic acid (0.05%) 183.7 Adenosine (0.04%) + D-panthenol (0.06%) + niacinamide (0.1%) + Echo extract (0.05%) + zinc pyrithione (0.05%) 186.2 Adenosine (0.04%) + D-panthenol (0.06%) + niacinamide (0.1%) + Echo extract (0.05%) + salicylic acid (0.05%) 197.3

When looking at the results disclosed in Table 5 above, when treating with a mixture of adenosine, D-panthenol, and niacinamide with one or more of Sauerkraut extract, Echo extract, zinc pyrithione, and salicylic acid, dermal papilla cell mitochondrial activity It was confirmed that a significant synergistic effect occurred in the enhancement.

Experimental Example 3: Effect of dermal papilla cell activation signal (Wnt/β-catenin) regulation

In general, the Wnt/β-catenin signal activated in the dermal papilla occurs during the hair growth cycle, when transitioning from the catagen to the active phase, that is, from the beginning of hair growth to the active phase in which hair growth occurs. During the resting and degenerative phases, the Wnt/β-catenin signal weakens or disappears, causing the hair follicles to degenerate and hair loss. Therefore, in the mixture of adenosine, D-panthenol, and niacinamide of the present disclosure, the treatment group of at least one of sewage extract, seaweed extract, zinc pyrithione, and salicylic acid contributes to Wnt/β-catenin signal amplification through this experiment. Confirmed.

As a positive control to amplify the Wnt/β-catenin signal, wnt3a protein was used, and as a negative control, DMSO was used. ^{After seeding 3 x 10 4} Wnt Reporter HEK293A cells in each well of a 96-well plate in culture, adenosine, D-panthenol, and niacinamide of the present disclosure were added to the mixture of sauerkraut extract, seaweed extract, zinc pyrithione, and salicylic acid. At least one type was treated as shown in Table 6 below. Then, reporter assay was performed using Promega's Luciferase assay kit (E1960). The manufacturer's experimental method was followed, and Wnt/β-catenin promoter activity was measured using a luminometer Victor (PerkinElmer, Waltham, Massachusetts, USA).

treatment group Wnt/β-catenin signal activation ability for hair follicle activation (%) untreated group 100 100 ng/ml recombinant wnt3a 248.9 Adenosine (0.06%) + D-panthenol (0.08%) + Niacinamide (0.16%) 1363.4 Adenosine (0.04%)+D-panthenol (0.06%)+niacinamide (0.1%)+Sewage extract (0.1%) 1590.1 Adenosine (0.04%) + D-panthenol (0.06%) + niacinamide (0.1%) + Echo extract (0.1%) 1530.4 Adenosine (0.04%) + D-panthenol (0.06%) + Niacinamide (0.1%) + Zinc pyrithione (0.1%) 1571.0 Adenosine (0.04%)+D-panthenol (0.06%)+niacinamide (0.1%)+salicylic acid (0.1%) 1573.1 Adenosine (0.04%)+D-panthenol (0.06%)+niacinamide (0.1%)+Sewage extract (0.05%)+Zinc pyrithione (0.05%) 1825.9 Adenosine (0.04%) + D-panthenol (0.06%) + niacinamide (0.1%) + sewage extract (0.05%) + salicylic acid (0.05%) 1697.7 Adenosine (0.04%) + D-panthenol (0.06%) + niacinamide (0.1%) + Echo extract (0.05%) + zinc pyrithione (0.05%) 1794.8 Adenosine (0.04%) + D-panthenol (0.06%) + niacinamide (0.1%) + Echo extract (0.05%) + salicylic acid (0.05%) 1889.7

When looking at the results disclosed in Table 6 above, when treating a mixture of adenosine, D-panthenol, and niacinamide with at least one of sewage extract, Echo extract, zinc pyrithione, and salicylic acid, Wnt/β- It was confirmed that the activity of the catenin signaling system was synergistically enhanced. Since the Wnt/β-catenin signal is a hair growth promoting signal of hair follicle stem cells, it can be seen from the experimental results that the composition of the present disclosure exhibits a remarkable synergistic effect on hair growth promotion.

Experimental Example 4: Hair growth effect of composition 1 (Hair Tonic) for hair loss treatment or hair growth promotion

The hair growth effect of the composition 1 (Hair Tonic) for hair loss treatment or hair growth promotion of the present invention was tested on a total of 70 men and women with significantly less hair than normal or weak hair with symptoms of hair loss. A total of 70 males and females were divided into 7 groups of 10 each, and Comparative Examples 1 and 2 and Examples 1 to 5 were used, and used 5 times a week for hair and scalp for 6 months. After use, researcher evaluation through clinical photography and evaluation through phototricogram were conducted. The researcher's evaluation through clinical photos was evaluated with three items of 'good', 'slight', or 'no change' for Comparative Examples and Examples 2 and 6 months after application (Good: 50% to 75% improvement, Slight: 25%~50% improvement, No change: no change). The evaluation of the number of hairs per unit area and the average hair thickness through the phototricogram was measured for Comparative Examples and Examples 6 months after application, and the results are shown in Table 7.

sample Researcher evaluation through clinical pictures phototricogram no change Slight Good Number of hairs per unit area (pieces/cm ² ) Average hair thickness (μm) (number of people) (number of people) (number of people) February June February June February June october June october June Comparative Example 1 8 7 2 3 0 0 125.2±20 126.9±14 43.6±8 45.2±3 Comparative Example 2 7 4 2 4 One 2 125±16 134±10 45.3±6 55.9±4 Example 1 6 4 3 3 One 3 119±10 135±10 44.2±10 53.4±3 Example 2 7 3 2 4 One 3 128.2±17 137±23 44.7±8 54.3±4 Example 3 3 2 5 4 2 4 124.4±15 153±17 41.3±7 62.5±2 Example 4 2 2 4 3 4 5 123.1±13 179±19 44±8 77.2±8 Example 5 2 One 4 4 4 5 120±20 169±17 42.5±4 69±6

From the results disclosed in Table 7, it was confirmed that the number of hairs per unit area and the average hair thickness were improved when the treatment was performed with a mixture of adenosine, D-panthenol and niacinamide. In particular, it was confirmed that when adenosine, D-panthenol and niacinamide were mixed in a specific weight ratio and treated according to the present disclosure, the effect of improving the number of hairs per unit area and the average hair thickness was significantly improved.

Experimental Example 5: Hair growth effect of composition 2 (Hair Tonic) for hair loss treatment or hair growth promotion

The hair growth effect of the composition 2 (Hair Tonic) for treating hair loss or for promoting hair growth of the present invention was tested on a total of 60 men and women with significantly less hair than normal or weak hair with symptoms of hair loss. A total of 60 males and females were divided into 6 groups of 10 each, and Comparative Example 1 and Examples 6 to 9 were used, and used 5 times a week for hair and scalp for 6 months. After use, researcher evaluation through clinical photography and evaluation through phototricogram were conducted. The researcher's evaluation through clinical photos was evaluated with three items of 'good', 'slight', or 'no change' for Comparative Examples and Examples 2 and 6 months after application (Good: 50% to 75% improvement, Slight: 25%~50% improvement, No change: no change). The evaluation of the number of hairs per unit area and the average hair thickness through the phototricogram was measured for Comparative Examples and Examples 6 months after application, and the results are shown in Table 8.

sample Researcher evaluation through clinical pictures phototricogram no change Slight Good Number of hairs per unit area (pieces/cm2) Average hair thickness (μm) (number of people) (number of people) (number of people) February June February June February June october June october June Comparative Example 1 9 8 One 2 0 0 133.2±10 130.8±10 40.6±4 40.4±6 Example 6 One One 3 4 6 5 131.8±10 199±13 44.5±3 86.9±3 Example 7 One 0 One 2 8 8 129.8±19 188±18 42.8±5 88.3±7 Example 8 2 0 4 3 4 7 134.5±10 185±19 45.4±8 83.5±9 Example 9 0 0 3 2 7 8 132.3±18 190±17 43.3±2 86±2

When looking at the results disclosed in Table 8 above, as in Examples 6 to 9, the mixture of adenosine, D-panthenol and niacinamide was treated by additionally including one or more of sewage extract, seaweed extract, zinc pyrithione, and salicylic acid. In this case, it was confirmed that the number of hairs per unit area and the average hair thickness were significantly increased compared to Examples 1 to 5, thereby exhibiting a remarkable synergistic effect in preventing hair loss and promoting hair growth.

Formulation examples such as the hair tonic or hair lotion are only examples of the composition for preventing hair loss or promoting hair growth of the present disclosure, and it is common in the art to which the present invention pertains that the scope of the composition of the present disclosure is not limited only to these formulations. It will be obvious to those with knowledge.

Claims (11)

mixtures of adenosine, D-panthenol and niacinamide; and
A composition for preventing hair loss or promoting hair growth, comprising, as an active ingredient, any one or more selected from the group consisting of sauerkraut extract, eosinosa extract, zinc pyrithione and salicylic acid. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the content of the active ingredient is 0.0001 to 10% by weight based on the total weight of the composition. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition enhances the activity of dermal papilla cells. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition promotes mitochondrial activity of dermal papilla cells. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition promotes the activity of the Wnt/β-catenin signaling system. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition is a pharmaceutical composition. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition is a composition for quasi-drugs. The composition for preventing hair loss or promoting hair growth according to claim 1, wherein the composition is a cosmetic composition. A product for hair or scalp comprising the composition for preventing hair loss or promoting hair growth according to claim 1. 10. The method of claim 9, wherein the product is a hair growth agent, scalp clinic agent, scalp scaling agent, scalp massage agent, scalp care agent, detergent, shampoo, tonic, hair conditioner, hair lotion, gel, pack, cream, essence, powder, spray , a product for hair or scalp selected from the group consisting of oils, soaps, ointments, hair styling agents, hair dyes and perms. In the composition for preventing hair loss or promoting hair growth comprising a mixture of adenosine, D-panthenol and niacinamide as an active ingredient,
The composition for preventing hair loss or promoting hair growth, characterized in that the weight ratio of the mixture is 1: 0.3-5: 0.2-8 (adenosine: D-panthenol: niacinamide).