CN111213603A - Ecological breeding method for Penaeus vannamei Boone in rough labeling stage - Google Patents

Ecological breeding method for Penaeus vannamei Boone in rough labeling stage Download PDF

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CN111213603A
CN111213603A CN201811412723.7A CN201811412723A CN111213603A CN 111213603 A CN111213603 A CN 111213603A CN 201811412723 A CN201811412723 A CN 201811412723A CN 111213603 A CN111213603 A CN 111213603A
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water
pond
days
bacillus
feeding
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周可
谢凤行
张峰峰
赵琼
赵玉洁
魏士旺
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TIANJIN AGRICULTURAL BIO-TECH RESEARCH CENTER
Tianjin Baoyide Agricultural Technology Co Ltd
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TIANJIN AGRICULTURAL BIO-TECH RESEARCH CENTER
Tianjin Baoyide Agricultural Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/59Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F9/00Multistage treatment of water, waste water or sewage
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/001Processes for the treatment of water whereby the filtration technique is of importance
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F1/52Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00Treatment of water, waste water, or sewage
    • C02F2001/007Processes including a sedimentation step
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention discloses an ecological breeding method for Penaeus vannamei Boone in the rough marking stage, which comprises the following steps: (1) laying an anti-leakage film and a nano oxygenation pipe at the bottom of the coarse marking tank; (2) storing and culturing water, introducing qualified outside pond water into a standard coarse pond after precipitation and sand filtration, wherein the water storage depth is 0.4-1.5m, and adding photosynthetic bacteria, bacillus, chlorella and brown sugar to culture water after the outside pond water is stored and exposed for 3 days; (3) seedling releasing, selecting healthy young shrimp, and seedling releasing density is 3000-3500 tail/m3A body of water; (4) performing standard rough culture, wherein the culture period is 15-20 days, feeding once every 4 hours, uniformly stirring the opening material and the fairy shrimp with lactic acid bacteria, and then feeding, and adding VC and immune polysaccharide during the feeding period; during the cultivation period, chlorella, photosynthetic bacteria and bacillus are used for regulating water quality. The shrimp larvae marked to be coarse by the method have high survival rate and good vitality, and water and antibiotics do not need to be added in the whole culture periodPlain and disinfectant to achieve the purpose of ecological breeding.

Description

Ecological breeding method for Penaeus vannamei Boone in rough labeling stage
Technical Field
The invention belongs to the field of aquaculture, and relates to an ecological breeding method for the standard-coarsening stage of Penaeus vannamei Boone.
Background
The penaeus vannamei boone is introduced into China in the eighties of the last century, and the culture area is gradually enlarged due to the advantages of quick growth, strong adaptability, delicious meat quality and the like, so that the penaeus vannamei boone has become the pillar industry of aquaculture in China. However, if the post larvae with abnormal fertilized eggs are directly thrown into an external pond, the post larvae are small and weak, so that the survival rate is low and the cost of the post larvae is high. If the size of the young shrimps is 2-3 centimeters, the young shrimps are put into an outer pond, the survival rate is greatly improved, but in the existing standard coarse breeding, part of farmers pursue large seedlings at a time, and the phenomena of excessive feeding, water quality deterioration, drug abuse, physique slipping and no obvious increase of survival rate after the young shrimps are put into the outer pond exist.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides an ecological breeding method for the standard-coarsening stage of the Penaeus vannamei Boone. The method takes the microecological preparation as the core, can effectively purify water quality, construct intestinal flora of the juvenile shrimps, improve the survival rate and the growth rate, cultivate the juvenile shrimps with strong physique and strong anti-stress capability and improve the culture benefit under the condition of not using chemical drugs.
In order to achieve the purpose, the invention adopts the technical scheme that: an ecological breeding method for the standard coarse stage of the Penaeus vannamei Boone is characterized by comprising the following steps:
(1) preparation of coarse calibration pool
The standard coarse pond area is 10m multiplied by 40m, the underground soil pond is high in south, low and north, the side of the pond is about a 45-degree slope, and the underground soil pond is provided with a water adding and sewage discharging facility and a seedling growing pond. Draining water before seeding, sun drying for more than one week, spreading an anti-leakage film at the bottom of the pond, spreading a nanometer oxygenation pipe, supporting the pond with arched metal, covering with plastic film, sealing, and keeping warm.
(2) Water storage and cultivation
Detecting the indexes of the external pond water such as sub-salt, ammonia nitrogen, pathogenic microorganism and the like, introducing the qualified external pond water into a coarse shed after precipitation and sand filtration, and if the external pond water is fresh waterAdding halogen salt or sea crystal into water source, and regulating salinity to 1.5-2.0%. The water storage depth is 0.4-1.5m, photosynthetic bacteria, bacillus, chlorella and brown sugar are added after the water storage is exposed to the sun for 3 days, so that the concentration of algae cells in the water body is 107CFU/L, concentration of photosynthetic bacteria and Bacillus is 108CFU/L, and culturing for 2-3 d.
(3) Seedling setting
Selecting young shrimp with no parasite, no pathogenic bacteria, strong adverse water ability, full intestinal tract, black liver, and thick seedling 5 days after the growth of young shrimp, and the seedling density of 3000-3500 tail/m3A body of water.
(4) Coarse cultivation in the dark
Feeding once every 4 hours, wherein the feeding amount of each time is about 2-3% of the weight of the prawn seedlings, feeding commercial prawn seedling opening material and fairy shrimp, uniformly mixing the opening material and the fairy shrimp with lactic acid bacteria, then feeding (the mixing feeding amount of the lactic acid bacteria is 300-400mL), adding immune polysaccharide and VC during the culture period to reduce stress, and performing strengthening treatment on the prawn seedlings by using VC (400-500mL each time) 3-4 days before emergence of seedlings.
The water for seedling culture is precipitated and sand-filtered, and the water quality index and the pathogenic microorganism index are checked before water enters; measuring ammonia nitrogen, sub-salt and pH of the water body every day during seedling raising; adding chlorella (2.5kg), photosynthetic bacteria (2.5kg), bacillus (1.0kg) and brown sugar (1.0kg) once every 2-3 days; the water is replenished every 2-3 days, the water is not changed in the period, and no antibiotics or disinfectants are used.
The invention has the advantages that the seedling culture water body is subjected to precipitation and sand filtration treatment, and probiotics are used for culturing water, adjusting water, mixing materials and biological baits, so that the content of harmful substances in the water body can be effectively removed, the healthy microbial flora of the water body and the shrimp intestinal tract can be established, the feed digestibility can be improved, the discharge of nitrogen and phosphorus pollutants can be reduced, the water is not changed in the culture process, antibiotics and disinfectants are not used, and the aim of ecological culture is fulfilled.
Detailed Description
The present invention is described below with reference to examples, which are not intended to limit the present invention, and those skilled in the art may make modifications and variations thereto in light of the spirit of the present invention, which should be construed as being within the scope of the present invention, the scope and spirit of the present invention being defined by the appended claims.
Example 1
(1) Water storage and cultivation: the area of No. 1 shed is 10m × 40m, the north is high, the south is low, the underground soil pool is covered with an anti-seepage film at the bottom of the pool, and the nano-tubes are paved on the film. Storing water for 4 months and 20 days, wherein the water source is external pond water, each index is qualified after detection, the water storage depth is 40cm north and 1.2m south after precipitation and sand filtration treatment, and the water is exposed for 3 days after water storage. 4 months and 23 days, 5kg of photosynthetic bacteria, 5kg of chlorella and 1kg of bacillus (1 kg of brown sugar and 100L of water are added for activation and then are sprinkled).
(2) Seedling releasing: about 10 am at 4 months and 25 days, selecting the shrimps which are free of parasites, do not carry pathogenic bacteria, have strong reverse water capacity, are full and have complete gut and black livers, and are thrown 5 days after the shrimps change from the mysid period, wherein the number of the shrimps is about 9.2 ten thousand tails/kg, the number of the shrimps is 13 kg, and the throwing amount is 120 ten thousand tails.
(3) Feeding: feeding at a fixed time and a fixed point in a quantitative feeding mode at 2:00, 6:00, 14:00, 18:00 and 22:00 every day, wherein the feeding amount is 2-3 percent of the weight of the shrimps.
TABLE 11 cultivation situation
Figure BDA0001876773350000031
(4) Water quality control
Respectively adding 5kg of photosynthetic bacteria, 5kg of chlorella and 1kg of bacillus into the culture pond for regulating water quality at 29 days in 4 months, 1 day in 5 months, 3 days in 5 months and 6 days in 5 months, directly splashing the photosynthetic bacteria and the chlorella, and splashing the bacillus after activating the bacillus for 3-4h by adding 1kg of brown sugar and 100L of culture pond water. During the whole coarse calibration period, the pH value of the water body is about 8.5, the ammonia nitrogen is less than 0.2mg/L, and the nitrite is less than 0.03 mg/L.
(5) Emergence of seedlings
Feeding once at 6 am in 8 months in the morning, and dewatering to sprout at 8 am, wherein 90kg sprouts, about 1.3 thousand tails/kg, 117 thousand tails sprout in month, the survival rate is 97.5%, and the shrimp larvae have strong activity and do not carry pathogenic bacteria.
Example 2
(1) Water storage and cultivation: the area of No. 2 shed is 10m × 40m, the north is high, the south is low, the underground soil pool is covered with anti-seepage film, and the nano tube is paved on the film. Storing water for 4 months and 20 days, wherein the water source is external pond water, each index is qualified after detection, the water storage depth is 45cm north and 1.5m south after precipitation and sand filtration treatment, and the water is exposed for 3 days after water storage. 4 months and 23 days, 5kg of photosynthetic bacteria, 5kg of chlorella and 1kg of bacillus (1 kg of brown sugar and 100L of water are added for activation and then are sprinkled).
(2) Seedling releasing: about 10 am at 4 months and 25 days, selecting the shrimps which are free of parasites, do not carry pathogenic bacteria, have strong reverse water capacity, are full and have complete gut and black livers, and are thrown 5 days after the shrimps change from the mysid period, wherein the number of the shrimps is about 9.2 ten thousand tails/kg, the number of the shrimps is 13 kg, and the throwing amount is 120 ten thousand tails.
(3) Feeding: feeding at a fixed time and a fixed point in a quantitative feeding mode at 2:00, 6:00, 14:00, 18:00 and 22:00 every day, wherein the feeding amount is 2-3 percent of the body weight.
TABLE 22 Pond culture conditions
Figure BDA0001876773350000041
(4) Water quality control
Respectively adding 5kg of photosynthetic bacteria, 5kg of chlorella and 1kg of bacillus into the culture pond for regulating water quality at 29 days in 4 months, 1 day in 5 months, 3 days in 5 months and 6 days in 5 months, directly splashing the photosynthetic bacteria and the chlorella, and splashing the bacillus after activating the bacillus for 3-4h by adding 1kg of brown sugar and 100L of culture pond water. The pH value of the water body in the whole coarse calibration period is about 8.5, the ammonia nitrogen is less than 0.2mg/L, and the nitrite is less than 0.03 mg/L.
(5) Emergence of seedlings
5, 8 days, seedlings emerge with rainfall at 10 am, 91kg of seedlings emerge together, about 1.32 thousand tails/kg, 120 ten thousand tails emerge in each month, the survival rate is 100 percent, and the shrimp larvae have strong vitality and do not carry pathogenic bacteria.
Example 3
(1) Water storage and cultivation: the area of No. 3 shed is 10m × 40m, the north is high, the south is low, the underground soil pool is covered with anti-seepage film, and the nano tube is paved on the film. Storing water for 4 months and 20 days, wherein the water source is external pond water, each index is qualified after detection, the water storage depth is 50cm north and 1.3m south after precipitation and sand filtration treatment, and the water is exposed for 3 days after water storage. 4 months and 23 days, 5kg of photosynthetic bacteria, 5kg of chlorella and 1kg of bacillus (1 kg of brown sugar and 100L of water are added for activation and then are sprinkled).
(2) Seedling releasing: about 10 am at 4 months and 27 days, selecting shrimps which are free of parasites, do not carry pathogenic bacteria, have strong reverse water capacity, are full and have complete gut and black livers, and are thrown 5 days after the shrimps change from the mysid period, wherein the throw amount is about 10.4 ten thousand tails/kg, 12.5 kg and 130 ten thousand tails.
(3) Feeding: feeding at a fixed time and a fixed point in a quantitative feeding mode at 2:00, 6:00, 14:00, 18:00 and 22:00 every day, wherein the feeding amount is 2-3 percent of the body weight.
Table 33 Pond culture conditions
Figure BDA0001876773350000051
(4) Water quality control
Respectively adding 5kg of photosynthetic bacteria, 5kg of chlorella and 1kg of bacillus into the culture pond for regulating water quality at 28 days in 4 months, 1 day in 5 months, 4 days in 5 months and 6 days in 5 months, directly splashing the photosynthetic bacteria and the chlorella, and splashing the bacillus after activating the bacillus for 3-4h by adding 1kg of brown sugar and 100L of culture pond water. The pH value of the water body in the whole coarse calibration period is about 8.5, the ammonia nitrogen is less than 0.2mg/L, and the nitrite is less than 0.03 mg/L.
(5) Emergence of seedlings
3, 5 months and 9 days, and 2 pm, the seedlings emerge by precipitation, wherein the seedlings emerge 103kg, about 1.14 thousands of seedlings per kilogram, the seedlings emerge 117.42 thousands of seedlings in months, the survival rate is 90%, and the shrimp seedlings have strong activity and do not carry pathogenic bacteria.
Example 4
(1) Water storage and cultivation: the area of No. 4 shed is 10m × 40m, the north is high, the south is low, the underground soil pool is covered with anti-seepage film, and the nano tube is paved on the film. Storing water for 20 days in 4 months, wherein the water source is external pond water, each index is qualified after detection, the water storage depth is 50cm north and 1.4m south after precipitation and sand filtration treatment, and the water is exposed for 3 days after water storage. 4 months and 23 days, 5kg of photosynthetic bacteria, 5kg of chlorella and 1kg of bacillus (1 kg of brown sugar and 100L of water are added for activation and then are sprinkled).
(2) Seedling releasing: about 10 am at 4 months and 27 days, selecting shrimps which are free of parasites, do not carry pathogenic bacteria, have strong reverse water capacity, are full and have complete gut and black livers, and are thrown 5 days after the shrimps change from the mysid period, wherein the throw amount is about 10.4 ten thousand tails/kg, 12.5 kg and 130 ten thousand tails.
(3) Feeding: feeding at a fixed time and a fixed point in a quantitative feeding mode at 2:00, 6:00, 14:00, 18:00 and 22:00 every day, wherein the feeding amount is 2-3 percent of the body weight.
Table 44 pond culture conditions
Figure BDA0001876773350000061
(4) Water quality control
Respectively adding 5kg of photosynthetic bacteria, 5kg of chlorella and 1kg of bacillus into the culture pond for regulating water quality at 28 days in 4 months, 1 day in 5 months, 4 days in 5 months and 6 days in 5 months, directly splashing the photosynthetic bacteria and the chlorella, and splashing the bacillus after activating the bacillus for 3-4h by adding 1kg of brown sugar and 100L of culture pond water. The pH value of the water body in the whole coarse calibration period is about 8.5, the ammonia nitrogen is less than 0.2mg/L, and the nitrite is less than 0.03 mg/L.
(5) Emergence of seedlings
At noon 5 months and 8 days, the seedlings emerge by precipitation, the seedlings emerge 90kg together, about 1.3 thousand tails/kg, the seedlings emerge 117 ten thousand tails per month, the survival rate is 90%, and the shrimp larvae have strong vitality and do not carry pathogenic bacteria.

Claims (6)

1. An ecological breeding method for the standard crude stage of the Penaeus vannamei Boone is characterized by comprising the following steps;
(1) preparing a coarse calibration pool;
(2) storing and cultivating water;
(3) putting seedlings;
(4) and (3) coarse breeding: the cultivation period is 15-20d, feeding is carried out once every 4h, the materials are mixed by lactic acid bacteria, immune polysaccharide and VC are added, water is adjusted by chlorella, photosynthetic bacteria and bacillus in the cultivation process, water is supplemented every 2-3d, and water is not changed.
2. The ecological breeding method for the standard rough stage of the Penaeus vannamei Boone as claimed in claim 1, which is characterized in that: (1) the underground soil pond with the standard coarse pond area of 10m multiplied by 40m has the height from south to north, the slope of 45 degrees at the side of the pond, a water adding and sewage discharging facility and a seedling growing pond. Laying an anti-leakage film at the bottom of the tank, laying a nano oxygenation pipe, supporting the upper part of the tank by using arched metal, covering a plastic film, sealing and preserving heat.
3. The ecological breeding method for the standard rough stage of the Penaeus vannamei Boone as claimed in claim 1, which is characterized in that: (2) the water storage and culture method comprises the following steps: and (3) detecting indexes such as sub-salt, ammonia nitrogen, pathogenic microorganisms and the like of the external pond water, introducing the qualified external pond water into a standard coarse pond after precipitation and sand filtration, and if the external pond water is a fresh water source, adding halogen salt or seawater crystal, and adjusting the salinity to 1.5-2.0%. The water storage depth is 0.4-1.5m, photosynthetic bacteria, bacillus, chlorella and brown sugar are added after the water storage and the insolation for 3 days, so that the concentration of algae cells in the water body is 107CFU/L, concentration of photosynthetic bacteria and Bacillus is 108CFU/L, and culturing for 2-3 d.
4. The ecological breeding method for the standard rough stage of the Penaeus vannamei Boone as claimed in claim 1, which is characterized in that: (3) the young seed is selected from young shrimp with no parasite, no pathogenic bacteria, strong adverse water ability, full intestinal tract, black liver, and young shrimp 5 days after the young shrimp changes from Mysidacea stage, and the seedling density is 3000-3500 tail/m3A body of water.
5. The ecological breeding method for the standard rough stage of the Penaeus vannamei Boone as claimed in claim 1, which is characterized in that: (4) the medium breeding period is 15-20d, feeding is carried out once every 4h, the feeding amount of each time is about 2% -3% of the weight, opening materials and fairy shrimp larvae are fed, the opening materials and the fairy shrimp larvae are uniformly mixed by lactic acid bacteria and then fed (the mixing feeding amount of the lactic acid bacteria is 300-400mL each time), immune polysaccharide and VC are added during the breeding period to reduce stress, VC (400-500mL each time) is used 3-4d before emergence of seedlings, and the prawn larvae are subjected to strengthening treatment.
6. The ecological breeding method for the standard rough stage of the Penaeus vannamei Boone as claimed in claim 1, which is characterized in that: (4) the water quality regulation and control method comprises the following steps: the water for seedling culture is precipitated and sand-filtered, and the water quality index and the pathogenic microorganism index are checked before water enters; measuring ammonia nitrogen, sub-salt and pH of the water body every day during seedling raising; adding chlorella (2.5kg), photosynthetic bacteria (2.5kg), bacillus (1.0kg) and brown sugar (1.0kg) once every 2-3 days; the water is replenished every 2-3 days, the water is not changed in the period, and no antibiotics or disinfectants are used.
CN201811412723.7A 2018-11-23 2018-11-23 Ecological breeding method for Penaeus vannamei Boone in rough labeling stage Pending CN111213603A (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112369357A (en) * 2020-11-03 2021-02-19 福建闽业生物技术有限公司 Method for culturing shrimps in muddy water

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