CN111213512A - Planting method for improving total flavone content and antioxidant activity of wampee fruits - Google Patents
Planting method for improving total flavone content and antioxidant activity of wampee fruits Download PDFInfo
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- CN111213512A CN111213512A CN202010031532.7A CN202010031532A CN111213512A CN 111213512 A CN111213512 A CN 111213512A CN 202010031532 A CN202010031532 A CN 202010031532A CN 111213512 A CN111213512 A CN 111213512A
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- wampee
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- sodium bisulfite
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
Abstract
The invention discloses a planting method for improving the content of total flavonoids in wampee fruits and the antioxidant activity, and belongs to the technical field of fruit tree cultivation. Spraying sodium bisulfite to the Chinese wampee during the growth and development of Chinese wampee fruit. The specific use mode is that sodium bisulfite is prepared into the mass concentration of 600-1000 mg.L from 30 days after the yellow skin is withered‑1The aqueous solution of (A) is sprayed by a sprayer for 3-4 times, wherein the spraying interval is 7-10 days every time, and the leaf wetting degree is adopted for each time; other production management is carried out according to the daily management of a normal wampee garden. The method is simple and easy to operate, has low cost, and can effectively improve the total flavone content of the wampee fruits and the wampee fruitsThe antioxidant activity, thus greatly improving the medicinal health care function of the wampee fruits, having good application prospect and being worth popularizing and using.
Description
Technical Field
The invention belongs to the technical field of fruit tree cultivation, and particularly relates to a planting method for improving the content of total flavonoids in wampee fruits and the antioxidant activity.
Background
Clausena lansium (also known as King jar, Huangkui, Huang dui, Huang Feng, Huang Dan, is a plant of the genus Clausena of the family Rutaceae, and a fruit tree of the tropical subtropical evergreen color. The clausena lansium is native to south China, has been cultivated for more than 1500 years to date, and is mainly distributed in the Jinshajiang river valley areas of Guangdong, Guangxi, Hainan, Fujian, Yunnan, southern Guizhou, Sichuan and Taiwan in China. The wampee fruit is attractive in appearance, unique in flavor, sour, sweet and delicious, rich in sugar, organic acid, dietary fiber, various vitamins and trace elements and rich in nutrition. In addition, the wampee fruit also has important medicinal value, and the traditional Chinese medicine is used for treating cough, phlegm and asthma, cold, fever, difficult urination, malaria and other diseases. Therefore, as a functional health-care fruit, the Chinese wampee is more and more favored by consumers; under the current situation that the price of a large number of fruits in China fluctuates greatly, the sales price of the fresh wampee fruits steadily rises year by year, wherein the sales price of fine fruits is as high as 30-60 yuan/kg, and the fresh wampee fruits become a bright spot which is difficult to see in the fruit industry. Under the drive of high economic benefits, the yellow skin production in China is changed from the past sporadic planting after the anterior chamber to large-scale commercial planting, the cultivation area is gradually increased, and the development prospect is wide.
The total flavone is an important nutritional health-care component in the wampee fruit, has stronger antioxidant activity because the structure of the total flavone is commonly connected with functional groups such as phenolic hydroxyl, methoxyl, methyl, isopentenyl and the like, and has great cleaning capability on oxygen free radicals and lipid peroxidation. In recent years, flavonoids extracted from wampee have been proved to have the functions of delaying aging, inhibiting tumor cell proliferation, preventing neurodegenerative diseases and the like. Therefore, the method for improving the content of the total flavonoids in the wampee fruits can effectively improve the nutrition and health care functions and the edible value of the wampee fruits, and has important significance for improving the income of fruit growers and promoting the further development of the industry.
Disclosure of Invention
Aiming at the problems, the invention aims to provide a planting method for improving the total flavone content and the antioxidant activity of wampee fruits.
In order to achieve the purpose, the invention adopts the following technical scheme:
a planting method for improving the content of total flavonoids in wampee fruits and the antioxidant activity comprises the following steps: spraying sodium bisulfite aqueous solution on the Chinese wampee during the growth and development of the Chinese wampee fruit.
Preferably, the mass concentration of the sodium bisulfite aqueous solution is 600-1000 mg.L-1。
Preferably, the mass concentration of the sodium bisulfite aqueous solution is 800 mg.L-1。
Preferably, the planting method for improving the total flavone content and the antioxidant activity of the wampee fruits comprises the following steps: and (3) spraying the prepared sodium bisulfite aqueous solution with the concentration to the wampee tree 30 days after the wampee is subjected to flower withering, spraying for 3-4 times, wherein the spraying interval is 7-10 days every time, and the spraying is performed by taking the leaf surface wetting degree as each time.
Preferably, the clausena lansium variety is chicken heart clausena lansium or seedless clausena lansium.
The invention has the technical effects that: in the invention, the wampee tree is sprayed with the sodium bisulfite medicament to treat the wampee tree in the growth and development process of the wampee fruit, so that the purpose of obviously improving the content of total flavonoids and the antioxidant activity in the wampee fruit is achieved, and the nutrition and health care functions and the medicinal value of the wampee fruit are greatly improved. The method is simple and convenient to operate, does not generate any side effect, is low in implementation cost, has a wide market prospect, and is worthy of popularization and application.
Detailed Description
In order to make the above objects of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to specific examples, but the scope of the present invention is not limited to the following examples.
1. Test method
The specific implementation effect in the subsequent examples was examined by the following test methods.
1.1 preparation of sample extract: collecting fruit samples in the fruit mature period, removing cores, cutting into pieces and mixing uniformly. Accurately weighing 25g of sample to be detected, adding 300mL of precooled 70% v/v ethanol solution for high-speed homogenization, leaching overnight, then carrying out vacuum filtration, carrying out vacuum rotary evaporation and concentration on the filtrate at 45 ℃ until the total volume is less than 10mL, finally using 70% v/v ethanol to fix the volume to 25mL, subpackaging and storing in a refrigerator at-40 ℃ for later use.
1.2 determination of total flavone content: by using AlCl3The measurement was carried out by colorimetry. Adding 1.2mLH into 300 μ L of sample extractive solution2O and 120. mu.L of 5 wt% NaNO2Reacting the solution for 5min, and adding 90 mu L of 10 wt% AlCl3·6H2O solution, after 6min, 600 mu L of 1mol/L NaOH solution is added, distilled water is added to make up the total volume to be 3mL, the light absorption value is measured at 510nm, and 70% v/v ethanol solution is used as a blank control instead of a sample. A standard curve was plotted using Catechin (Catechin) as a standard substance, and the final result was expressed as the unit of mg CE/100g FW in terms of Catechin Equivalent (CE) per 100g of fresh sample.
1.3 determination of antioxidant Activity: the antioxidant activity is comprehensively evaluated by 2 methods of DPPH free radical scavenging capacity and FRAP antioxidant capacity respectively.
Determination of DPPH radical scavenging Capacity: adding 900 μ L of 80 μmol/L sample extractive solution 100 μ L-1The DPPH methanol solution is fully mixed, placed in a dark room for reaction for 30min, the light absorption value is measured at the wavelength of 517nm, a methanol solution with 70% v/v replaces a sample to be used as a blank, and the removal rate (%) is equal to (A) according to a formula0-A1)/A0X 100% calculation of DPPH clearance for samples, where A1Absorbance value after addition of sample, A0Absorbance of blank control. With ascorbic acid (As)corbic acid, AsA) was used as a standard substance, the removal rate of DPPH was measured in the same manner, a calibration curve was prepared, and the final result was expressed as Ascorbic acid equivalent (AsA equivalent) per 100g of fresh sample in mg asaequiv/100 g FW.
Determination of FRAP antioxidant capacity: collecting 100 μ L of sample extractive solution, adding 900 μ L of FRAP working solution (containing 300 mmol. L of FRAP working solution)-1pH 3.6 acetate buffer 25mL, 10 mmol. multidot.L-12.5mL of a solution of 2,4, 6-tripyridyl-s-triazine (TPTZ) in 20 mmol. multidot.L-1FeC13·6H22.5mL of O solution for use in preparation now), mixing uniformly, reacting in a water bath kettle at 37 ℃ for 40min in a dark place, measuring the light absorption value at the wavelength of 593nm, and replacing the sample with 70% v/v methanol solution as a blank. With FeSO4For the standard substance, a calibration curve was prepared in the same manner, and the final result was equivalent to Fe per 100g of fresh sample2+Equivalent weight (Fe)2+equivalent,Fe2+equiv.) in mmol of Fe2+equiv./100g FW。
Example 1
The test was carried out in 2017 in an orchard of Binxiang shun yellow-skinned professional cooperative society, Yunhuo city, Yunan county, Guangdong province, wherein the yellow-skinned variety (series) to be tested is Yunan yellow-skinned', 8 years of tree age, and the planting specification is 3m × 4 m. Spraying crown on the test and control trees with sprayer 30 days after blossom-1The sodium bisulfite aqueous solution is sprayed with clear water compared with the tree, the wampee leaves are sprayed to be wet each time, the sodium bisulfite aqueous solution is sprayed once every 7 days for 4 times, and other production management is carried out according to the daily management of a wampee garden.
When the fruits are ripe, 4 ears of ripe fruits are randomly collected from the east, south, west and north directions of each tree respectively, 5 fruits are randomly collected from each ear of fruit and mixed, and the total flavone content, DPPH free radical scavenging capacity and FRAP antioxidant capacity of the fruits are measured, and the results are shown in the following table 1:
TABLE 1
Note: the lower case letters in the same column represent significant differences at the 0.05 level.
As can be seen from Table 1, 600 mg.L was used-1Compared with a control, the total flavone content, DPPH removing capacity and FRAP oxidation resistance content of the ripe wampee fruit subjected to spraying treatment by the sodium bisulfite aqueous solution are respectively improved by 8.3%, 10.5% and 11.8%, and the difference reaches a significant level.
Example 2
The test was carried out in 2017 in an orchard of Binxiang shun yellow-skinned professional cooperative society, Yunhuo city, Yunan county, Guangdong province, wherein the yellow-skinned variety (series) to be tested is Yunan yellow-skinned', 8 years of tree age, and the planting specification is 3m × 4 m. Spraying crown of wampee test tree and control tree with sprayer 30 days after the wampee leaves, wherein the mass concentration of the sprayed tree is 800 mg.L-1The sodium bisulfite aqueous solution is sprayed with clear water compared with the tree, the wampee leaves are sprayed to be wet each time, the sodium bisulfite aqueous solution is sprayed once every 7 days for 3 times, and other production management is carried out according to the daily management of a wampee garden.
When the fruits are ripe, 4 ears of ripe fruits are randomly collected from the east, south, west and north directions of each tree respectively, 5 fruits are randomly collected from each ear of fruit and mixed, and the total flavone content, DPPH free radical scavenging capacity and FRAP antioxidant capacity of the fruits are measured, and the results are shown in the following table 2:
TABLE 2
Note: the lower case letters in the same column represent significant differences at the 0.05 level.
As can be seen from Table 2, 800 mg.L was used-1Compared with a control, the total flavone content, DPPH scavenging capacity and FRAP oxidation resistance content of the mature fruit after the spraying treatment of the sodium bisulfite aqueous solution are respectively improved by 17.0 percent, 20.9 percent and 21.7 percent, and the difference reaches a significant level.
Example 3
The test was carried out in 2017 in an orchard of Binxiang shun yellow-skinned professional cooperative society, Yunhuo city, Yunan county, Guangdong province, wherein the yellow-skinned variety (series) to be tested is Yunan yellow-skinned', 8 years of tree age, and the planting specification is 3m × 4 m. Spraying crown of wampee test tree and control tree with sprayer 30 days after the wampee leaves flowers, wherein the mass concentration of the sprayed test tree is 1000 mg.L-1The sodium bisulfite aqueous solution is sprayed with clear water compared with the tree, the wampee leaves are sprayed to be wet each time, the sodium bisulfite aqueous solution is sprayed once every 10 days for 4 times, and other production management is carried out according to the daily management of a wampee garden.
When the fruits are ripe, 4 ears of ripe fruits are randomly collected from the east, south, west and north directions of each tree respectively, 5 fruits are randomly collected from each ear of fruit and mixed, and the total flavone content, DPPH free radical scavenging capacity and FRAP antioxidant capacity of the fruits are measured, and the results are shown in the following table 3:
TABLE 3
Note: the lower case letters in the same column represent significant differences at the 0.05 level.
As can be seen from Table 3, 1000 mg.L was used-1Compared with a control, the total flavone content, DPPH scavenging capacity and FRAP oxidation resistance content of the mature fruit after the spraying treatment of the sodium bisulfite aqueous solution are respectively improved by 10.6 percent, 14.5 percent and 17.8 percent, and the difference reaches a significant level.
Example 4
The test was carried out in 2018 in orchard of Huazhou Yendong Huangpi specialty Co Ltd in Guangzhou, Guangdong province, the variety (system) of the yellow skin to be tested was ` Cor flavedo `, the age of the tree was 7 years, and the planting specification was 3m × 4 m. Spraying crown of wampee test tree and control tree with sprayer 30 days after the wampee leaves, wherein the mass concentration of the sprayed tree is 800 mg.L-1Spraying clear water to the control tree, spraying wampee leaf wetting every time, spraying once every 10 days for 3 times, and performing other production management according to daily management of wampee garden。
When the fruits are ripe, 4 ears of ripe fruits are randomly collected from the east, south, west and north directions of each tree respectively, 5 fruits are randomly collected from each ear of fruit and mixed, and the total flavone content, DPPH free radical scavenging capacity and FRAP antioxidant capacity of the fruits are measured, and the results are shown in the following table 4:
TABLE 4
Note: the lower case letters in the same column represent significant differences at the 0.05 level.
As can be seen from Table 4, 800 mg.L was used-1Compared with a control, the total flavone content, DPPH scavenging capacity and FRAP oxidation resistance content of the mature fruit after the spraying treatment of the sodium bisulfite aqueous solution are respectively improved by 14.2 percent, 19.0 percent and 18.2 percent, and the difference reaches a significant level.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.
Claims (5)
1. A planting method for improving the content of total flavonoids in wampee fruits and the antioxidant activity is characterized in that a sodium bisulfite aqueous solution is sprayed on wampee trees in the growth and development process of the wampee fruits.
2. The planting method according to claim 1, wherein the mass concentration of the sodium bisulfite aqueous solution is 600-1000 mg-L-1。
3. The planting method of claim 2, wherein the aqueous sodium bisulfite solution has a mass concentration of 800 mg-L-1。
4. The planting method according to any one of claims 1 to 3, wherein the wampee tree is sprayed with the sodium bisulfite aqueous solution with the adjusted concentration 3 to 4 times from 30 days after the wampee leaves wither, wherein the spraying interval is 7 to 10 days every time, and the spraying is performed until the leaf surface is wet.
5. The growing method according to any one of claims 1 to 3, wherein the species Clausena lansium is Cornus officinalis or Coreless Clausena lansium.
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