CN111213458A - Planting method of paeonia rockii - Google Patents
Planting method of paeonia rockii Download PDFInfo
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- CN111213458A CN111213458A CN201811327617.9A CN201811327617A CN111213458A CN 111213458 A CN111213458 A CN 111213458A CN 201811327617 A CN201811327617 A CN 201811327617A CN 111213458 A CN111213458 A CN 111213458A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G27/00—Self-acting watering devices, e.g. for flower-pots
- A01G27/04—Self-acting watering devices, e.g. for flower-pots using wicks or the like
- A01G27/06—Self-acting watering devices, e.g. for flower-pots using wicks or the like having a water reservoir, the main part thereof being located wholly around or directly beside the growth substrate
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C1/00—Ammonium nitrate fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a planting method of paeonia rockii. The method comprises the following steps: 1) preparing before planting, wherein 3 months before planting, deeply ploughing soil, exposing in the sun, and applying base fertilizer to the soil; 2) seedling raising, namely putting the pretreated paeonia rockii seeds into a seedling raising tray, and continuously culturing for 15 days after the seeds germinate to obtain seedlings; 3) and (5) planting and managing. The base fertilizer adopted by the invention takes green pollution-free straws, wheat bran and fresh walnut green husks as raw materials, so that the content of nutrients such as calcium, magnesium and the like in the base fertilizer is effectively improved, the water absorption and retention capacity of the base fertilizer is improved, the resources are reasonably utilized, the cost is reduced, and the ecological protection is facilitated.
Description
Technical Field
The invention relates to a planting method, in particular to a planting method of paeonia rockii.
Background
Paeonia rockii in gansu almost all show the basic characteristics of paeonia rockii: the plant is tall and big, the comfort is strong, the internode distance of the branch is long, the high growth quantity is large, and the annual branch of part of varieties can grow to 70 centimeters. The plant height is over 1 meter (part of varieties are up to 3 meters), the number of small leaves is more than 15, the leaves are small (anti-transpiration), the leaf backs are hairy, and the bases of the petals of all varieties have obvious large purple spots and purple erythema. Most of the flower hearts and ovaries are yellow white or white, and some of the flower hearts are purple red.
Because the paeonia rockii trees are tall (the trunk is 1m-3m tall), the flower quantity is large, and the flowering period is easily influenced by natural disasters such as strong wind and the like, the damage of branch breaking and even integral scraping of centuries old trees is caused. At present, only the wood strips are used for manual reinforcement, the workload is large, and certain influence is caused on ornamental flowers. Therefore, a planting method is urgently needed to optimize the strength of the branch and the trunk of the paeonia rockii.
Disclosure of Invention
The invention aims to provide a planting method of paeonia rockii.
In order to achieve the purpose, the planting method of paeonia rockii provided by the invention comprises the following steps:
1) preparation before planting:
3 months before planting, deeply ploughing the soil to a depth of 30-40 cm, insolating for 3-4 days in the sun, applying a base fertilizer to the soil according to 800 kg/mu, and applying a soil conditioner to the soil according to 1 kg/mu, wherein the base fertilizer is corn straw with a water content of 50-60% after crushing, wheat bran and fresh walnut green husk after crushing according to a mass ratio of 35: 50: 25 mixing, placing in nitrogen, fermenting at 45 deg.C for 2 days, at 55 deg.C for 2 days, and at 60 deg.C for 3 days; after fertilizing for 10 days, spraying magnetized water to the soil according to 300 kg/mu, after spraying for 2 days, paving buckwheat bran with the thickness of 2cm and wheat bran with the thickness of 1cm on the surface of the soil in sequence, wherein the water content of the buckwheat bran is 35-50%, the water content of the wheat bran is 30-33%, after spraying for 5 days, applying a compound microbial agent, wherein the compound microbial agent consists of a bacillus subtilis microbial agent, a rhodopseudomonas palustris microbial agent, a trichoderma harzianum microbial agent and a paecilomyces lilacinus microbial agent, the mass ratio of the four microbial agents is 2:2:1:2, and the total effective viable count of the compound microbial agent is 100 cfu/g;
2) seedling culture:
putting pretreated paeonia rockii seeds into a seedling raising tray, wherein a sandstone layer with the thickness of 1.5cm, a sponge water absorption layer with the thickness of 1cm and a black soil layer with the thickness of 3cm are sequentially paved in the seedling raising tray from bottom to top, and the sponge water absorption layer is composed of sponge water absorption layer particles with the diameter of 0.3 cm; keeping the room temperature at 25-30 ℃, and culturing for 15 days after the seeds germinate to obtain paeonia rockii seedlings; wherein the pretreatment specifically comprises the following steps: putting Paeonia suffruticosa seeds into clear water, treating for 30min in ultrasonic wave with frequency of 30KHz, draining, putting Paeonia suffruticosa seeds into 10% ammonium nitrate solution by mass percent, soaking for 3h, and irradiating for 3min in He-Ne laser with wavelength of 632.8 nm;
3) planting and managing: according to 9 strains/m2Planting the paeonia rockii seedlings in soil, watering at 6 points in the morning once every day within 10 days after planting, and applying white sugar powder to the roots of the paeonia rockii seedlings on the 2 nd day after planting, wherein the nutrient solution comprises ammonium nitrate, potassium nitrate and purified water in a mass ratio of 19:20: 500.
The magnetized water is obtained by magnetizing deep ocean water in a magnetic field with the magnetic field intensity of 0.3T.
In the step 3), white sugar powder is applied to the roots of the paeonia rockii seedlings according to the adding amount of 40g of each paeonia rockii seedling.
The buckwheat bran in the step 1) is buckwheat bran fermented at 40 ℃.
In the step 1), after deep ploughing, the starch grafted acrylate is put into the soil according to 100 kg/mu.
In the step 1), the preparation method of the compound microbial agent is characterized by comprising the following steps:
(1) preparation of a Single microbial inoculum
a. Slant culture: respectively inoculating bacillus subtilis, rhodopseudomonas palustris, trichoderma harzianum and paecilomyces lilacinus on a slant culture medium under an aseptic condition, and culturing for 36-48 hours at 35-37 ℃; the slant culture medium is as follows: 10-15g of glucose, 5-7g of peptone, 3-5g of beef extract, 2g of sodium chloride, 16g of agar powder and 1000mL of water, wherein the pH value is 7.0-7.5;
b. shake cultivation: inoculating the strain cultured in the step a into a shake flask culture medium under the aseptic condition, and carrying out shake culture for 12-18 hours at 35-37 ℃ under the condition of 180-200 r/min; the shake flask culture medium is as follows: 10-15g of glucose, 5-7g of peptone, 3-5g of beef extract, 1-3g of yeast extract, 2.5g of monopotassium phosphate, 5g of magnesium sulfate and 1000mL of water, wherein the pH value is 7.0-7.5;
c. culturing in a fermentation tank: b, inoculating the strain cultured in the step b into a fermentation medium under the aseptic condition, carrying out fermentation culture for 36-48 hours under the conditions of 0.5kg of tank pressure, 35-37 ℃ of temperature, 1:0.8-1.0 of ventilation capacity and 180-200r/min of rotation speed, and taking out of the tank when 80% of bacteria become spores to obtain fermentation liquor; the fermentation medium is as follows: 30-45g of sucrose, 20-25g of soybean meal, 10-15g of peptone, 5g of ammonium sulfate, 5-7g of fish meal, 5g of magnesium sulfate, 3g of manganese sulfate, 2.5g of monopotassium phosphate, 1.5g of calcium carbonate and 1000mL of water, wherein the pH value is 7.5-8.0;
d. and c, concentrating the fermentation liquor obtained in the step c, and drying to prepare spore powder to obtain the spore powder with the effective viable count of 100 hundred million cfu/g, namely respectively obtaining the bacillus subtilis microbial inoculum, the rhodopseudomonas palustris microbial inoculum, the trichoderma harzianum microbial inoculum and the paecilomyces lilacinus microbial inoculum.
The planting method of paeonia rockii provided by the invention has the beneficial effects that: the base fertilizer adopted by the invention takes green pollution-free straws, wheat bran and fresh walnut green husks as raw materials, so that the content of nutrients such as calcium, magnesium and the like in the base fertilizer is effectively improved, the water absorption and retention capacity of the base fertilizer is improved, the resources are reasonably utilized, the cost is reduced, and the ecological protection is facilitated; the organic waste is subjected to step fermentation, and fermentation is performed gradually, so that fermentation is more complete; according to the paeonia rockii planting method provided by the invention, the sponge water absorption layer is laid on the seedling raising plate, so that water can be absorbed and stored firstly, and then water is released in the planting process of the paeonia rockii, so that the balance of water in the planting process of the paeonia rockii is kept, and the survival rate and the growth rate of the planting of the paeonia rockii are improved; according to the method, the paeonia rockii seeds are subjected to ultrasonic treatment in the steps, so that the activity of the seeds is improved, and the seeds are promoted to germinate; the seeds are placed in He-Ne laser, so that the activity of enzymes in the seeds can be obviously improved, the metabolism is accelerated, and the germination rate is improved; white sugar powder is added according to the mass and applied to the roots of paeonia rockii seedlings; the penetration type of the seedling roots is increased, so that the nutrient substances can be quickly penetrated into the seedling roots, and the growth of the paeonia rockii seedlings is facilitated.
Detailed Description
Example 1
The planting method of paeonia rockii provided by the invention comprises the following steps:
1) preparation before planting:
3 months before planting, deeply ploughing the soil with the depth of 30cm, exposing the soil for 3-4 days in the sun, applying a base fertilizer to the soil according to 800 kg/mu, and applying a soil conditioner to the soil according to 1 kg/mu, wherein the base fertilizer is the crushed corn straws with the water content of 50%, wheat bran and the crushed fresh walnut green husks according to the mass ratio of 35: 50: 25 mixing, placing in nitrogen, fermenting at 45 deg.C for 2 days, at 55 deg.C for 2 days, and at 60 deg.C for 3 days; after fertilizing for 10 days, spraying magnetized water to the soil according to 300 kg/mu, after spraying for 2 days, paving buckwheat bran with the thickness of 2cm and wheat bran with the thickness of 1cm on the surface of the soil in sequence, wherein the water content of the buckwheat bran is 35%, the water content of the wheat bran is 30%, and after spraying for 5 days, applying a compound microbial agent, wherein the compound microbial agent consists of a bacillus subtilis microbial agent, a rhodopseudomonas palustris microbial agent, a trichoderma harzianum microbial agent and a paecilomyces lilacinus microbial agent, the mass ratio of the four microbial agents is 2:2:1:2, and the total effective viable count of the compound microbial agent is 100 cfu/g;
2) seedling culture:
putting pretreated paeonia rockii seeds into a seedling raising tray, wherein a sandstone layer with the thickness of 1.5cm, a sponge water absorption layer with the thickness of 1cm and a black soil layer with the thickness of 3cm are sequentially paved in the seedling raising tray from bottom to top, and the sponge water absorption layer is composed of a sponge layer with the thickness of 0.3 cm; keeping the room temperature at 25 ℃, and culturing for 15 days after the seeds germinate to obtain paeonia rockii seedlings; wherein the pretreatment specifically comprises the following steps: putting Paeonia suffruticosa seeds into clear water, treating for 30min in ultrasonic wave with frequency of 30KHz, draining, putting Paeonia suffruticosa seeds into 10% ammonium nitrate solution by mass percent, soaking for 3h, and irradiating for 3min in He-Ne laser with wavelength of 632.8 nm;
3) planting and managing: according to 9 strains/m2Planting the paeonia rockii seedlings in soil, watering at 6 points in the morning once every day within 10 days after planting, and applying white sugar powder to the roots of the paeonia rockii seedlings on the 2 nd day after planting, wherein the nutrient solution comprises ammonium nitrate, potassium nitrate and purified water in a mass ratio of 19:20: 500.
The magnetized water is obtained by magnetizing deep ocean water in a magnetic field with the magnetic field intensity of 0.3T.
In the step 3), white sugar powder is applied to the roots of the paeonia rockii seedlings according to the adding amount of 40g of each paeonia rockii seedling.
The buckwheat bran in the step 1) is buckwheat bran fermented at 40 ℃.
In the step 1), after deep ploughing, the starch grafted acrylate is put into the soil according to 100 kg/mu.
In the step 1), the preparation method of the compound microbial agent is characterized by comprising the following steps:
(1) preparation of a Single microbial inoculum
a. Slant culture: respectively inoculating bacillus subtilis, rhodopseudomonas palustris, trichoderma harzianum and paecilomyces lilacinus on a slant culture medium under an aseptic condition, and culturing for 36 hours at 35 ℃; the slant culture medium is as follows: 10g of glucose, 5g of peptone, 3g of beef extract, 2g of sodium chloride, 16g of agar powder and 1000mL of water, wherein the pH value is 7.0;
b. shake cultivation: b, inoculating the strain cultured in the step a into a shake flask culture medium under the aseptic condition, and carrying out shake culture for 12 hours at 35 ℃ under the condition of 180 r/min; the shake flask culture medium is as follows: 10g of glucose, 5g of peptone, 3g of beef extract, 1g of yeast extract, 2.5g of monopotassium phosphate, 5g of magnesium sulfate and 1000mL of water, wherein the pH value is 7.0;
c. culturing in a fermentation tank: b, inoculating the strain cultured in the step b into a fermentation medium under the aseptic condition, carrying out fermentation culture for 36 hours under the conditions of 0.5kg of tank pressure, 35 ℃ of temperature, 1:0.8 of ventilation capacity and 180r/min of rotation speed, and taking out of the tank when 80% of bacteria become spores to obtain fermentation liquor; the fermentation medium is as follows: 30g of cane sugar, 20g of soybean meal, 10g of peptone, 5g of ammonium sulfate, 5g of fish meal, 5g of magnesium sulfate, 3g of manganese sulfate, 2.5g of monopotassium phosphate, 1.5g of calcium carbonate and 1000mL of water, wherein the pH value is 7.5;
d. and c, concentrating the fermentation liquor obtained in the step c, and drying to prepare spore powder to obtain the spore powder with the effective viable count of 100 hundred million cfu/g, namely respectively obtaining the bacillus subtilis microbial inoculum, the rhodopseudomonas palustris microbial inoculum, the trichoderma harzianum microbial inoculum and the paecilomyces lilacinus microbial inoculum.
Example 2
The planting method of paeonia rockii provided by the invention comprises the following steps:
1) preparation before planting:
3 months before planting, deeply ploughing the soil to a depth of 40cm, insolating for 4 days in the sun, applying a base fertilizer to the soil according to 800 kg/mu, and applying a soil conditioner to the soil according to 1 kg/mu, wherein the base fertilizer is the crushed corn straws with a water content of 60%, wheat bran and the crushed fresh walnut green husks according to a mass ratio of 35: 50: 25 mixing, placing in nitrogen, fermenting at 45 deg.C for 2 days, at 55 deg.C for 2 days, and at 60 deg.C for 3 days; after fertilizing for 10 days, spraying magnetized water to the soil according to 300 kg/mu, after spraying for 2 days, paving buckwheat bran with the thickness of 2cm and wheat bran with the thickness of 1cm on the surface of the soil in sequence, wherein the water content of the buckwheat bran is 50%, the water content of the wheat bran is 33%, and after spraying for 5 days, applying a compound microbial agent, wherein the compound microbial agent consists of a bacillus subtilis microbial agent, a rhodopseudomonas palustris microbial agent, a trichoderma harzianum microbial agent and a paecilomyces lilacinus microbial agent, the mass ratio of the four microbial agents is 2:2:1:2, and the total effective viable count of the compound microbial agent is 100 cfu/g;
2) seedling culture:
putting pretreated paeonia rockii seeds into a seedling raising tray, wherein a sandstone layer with the thickness of 1.5cm, a sponge water absorption layer with the thickness of 1cm and a black soil layer with the thickness of 3cm are sequentially paved in the seedling raising tray from bottom to top, and the sponge water absorption layer is composed of a sponge layer with the thickness of 0.3 cm; keeping the room temperature at 30 ℃, and culturing for 15 days after the seeds germinate to obtain paeonia rockii seedlings; wherein the pretreatment specifically comprises the following steps: putting Paeonia suffruticosa seeds into clear water, treating for 30min in ultrasonic wave with frequency of 30KHz, draining, putting Paeonia suffruticosa seeds into 10% ammonium nitrate solution by mass percent, soaking for 3h, and irradiating for 3min in He-Ne laser with wavelength of 632.8 nm;
3) planting and managing: according to 9 strains/m2Planting the paeonia rockii seedlings in soil, watering at 6 points in the morning once every day within 10 days after planting, and applying white sugar powder to the roots of the paeonia rockii seedlings on the 2 nd day after planting, wherein the nutrient solution comprises ammonium nitrate, potassium nitrate and purified water in a mass ratio of 19:20: 500.
The magnetized water is obtained by magnetizing deep ocean water in a magnetic field with the magnetic field intensity of 0.3T.
In the step 3), white sugar powder is applied to the roots of the paeonia rockii seedlings according to the adding amount of 40g of each paeonia rockii seedling.
The buckwheat bran in the step 1) is buckwheat bran fermented at 40 ℃.
In the step 1), after deep ploughing, the starch grafted acrylate is put into the soil according to 100 kg/mu.
In the step 1), the preparation method of the compound microbial agent is characterized by comprising the following steps:
(1) preparation of a Single microbial inoculum
a. Slant culture: respectively inoculating bacillus subtilis, rhodopseudomonas palustris, trichoderma harzianum and paecilomyces lilacinus on a slant culture medium under an aseptic condition, and culturing for 48 hours at 37 ℃; the slant culture medium is as follows: 15g of glucose, 7g of peptone, 5g of beef extract, 2g of sodium chloride, 16g of agar powder and 1000mL of water, wherein the pH value is 7.5;
b. shake cultivation: inoculating the strain cultured in the step a into a shake flask culture medium under the aseptic condition, and carrying out shake culture for 18 hours at 37 ℃ under the condition of 200 r/min; the shake flask culture medium is as follows: 10-15g of glucose, 7g of peptone, 5g of beef extract, 3g of yeast extract, 2.5g of monopotassium phosphate, 5g of magnesium sulfate and 1000mL of water, wherein the pH value is 7.5;
c. culturing in a fermentation tank: b, inoculating the strain cultured in the step b into a fermentation medium under the aseptic condition, carrying out fermentation culture for 48 hours under the conditions of 0.5kg of tank pressure, 37 ℃ of temperature, 1:1.0 of ventilation capacity and 200r/min of rotation speed, and taking out of the tank when 80% of bacteria become spores to obtain fermentation liquor; the fermentation medium is as follows: 45g of cane sugar, 25g of soybean meal, 10-15g of peptone, 5g of ammonium sulfate, 7g of fish meal, 5g of magnesium sulfate, 3g of manganese sulfate, 2.5g of monopotassium phosphate, 1.5g of calcium carbonate and 1000mL of water, wherein the pH value is 8.0;
d. and c, concentrating the fermentation liquor obtained in the step c, and drying to prepare spore powder to obtain the spore powder with the effective viable count of 100 hundred million cfu/g, namely respectively obtaining the bacillus subtilis microbial inoculum, the rhodopseudomonas palustris microbial inoculum, the trichoderma harzianum microbial inoculum and the paecilomyces lilacinus microbial inoculum.
Example 3
The planting method of paeonia rockii provided by the invention comprises the following steps:
1) preparation before planting:
3 months before planting, deeply ploughing the soil to 35cm in depth, insolating for 3 days in the sun, applying a base fertilizer to the soil according to 800 kg/mu, and applying a soil conditioner to the soil according to 1 kg/mu, wherein the base fertilizer is the crushed corn straws with the water content of 55%, wheat bran and the crushed fresh walnut green husks according to the mass ratio of 35: 50: 25 mixing, placing in nitrogen, fermenting at 45 deg.C for 2 days, at 55 deg.C for 2 days, and at 60 deg.C for 3 days; after fertilizing for 10 days, spraying magnetized water to the soil according to 300 kg/mu, after spraying for 2 days, paving buckwheat bran with the thickness of 2cm and wheat bran with the thickness of 1cm on the surface of the soil in sequence, wherein the water content of the buckwheat bran is 40%, the water content of the wheat bran is 33%, and after spraying for 5 days, applying a compound microbial agent, wherein the compound microbial agent consists of a bacillus subtilis microbial agent, a rhodopseudomonas palustris microbial agent, a trichoderma harzianum microbial agent and a paecilomyces lilacinus microbial agent, the mass ratio of the four microbial agents is 2:2:1:2, and the total effective viable count of the compound microbial agent is 100 cfu/g;
2) seedling culture:
putting pretreated paeonia rockii seeds into a seedling raising tray, wherein a sandstone layer with the thickness of 1.5cm, a sponge water absorption layer with the thickness of 1cm and a black soil layer with the thickness of 3cm are sequentially paved in the seedling raising tray from bottom to top, and the sponge water absorption layer is composed of a sponge layer with the thickness of 0.3 cm; keeping the room temperature at 28 ℃, and culturing for 15 days after the seeds germinate to obtain paeonia rockii seedlings; wherein the pretreatment specifically comprises the following steps: putting Paeonia suffruticosa seeds into clear water, treating for 30min in ultrasonic wave with frequency of 30KHz, draining, putting Paeonia suffruticosa seeds into 10% ammonium nitrate solution by mass percent, soaking for 3h, and irradiating for 3min in He-Ne laser with wavelength of 632.8 nm;
3) planting and managing: according to 9 strains/m2Planting the paeonia rockii seedlings in soil, watering at 6 points in the morning once every day within 10 days after planting, and applying white sugar powder to the roots of the paeonia rockii seedlings on the 2 nd day after planting, wherein the nutrient solution comprises ammonium nitrate, potassium nitrate and purified water in a mass ratio of 19:20: 500.
The magnetized water is obtained by magnetizing deep ocean water in a magnetic field with the magnetic field intensity of 0.3T.
In the step 3), white sugar powder is applied to the roots of the paeonia rockii seedlings according to the adding amount of 40g of each paeonia rockii seedling.
The buckwheat bran in the step 1) is buckwheat bran fermented at 40 ℃.
In the step 1), after deep ploughing, the starch grafted acrylate is put into the soil according to 100 kg/mu.
In the step 1), the preparation method of the compound microbial agent is characterized by comprising the following steps:
(1) preparation of a Single microbial inoculum
a. Slant culture: respectively inoculating bacillus subtilis, rhodopseudomonas palustris, trichoderma harzianum and paecilomyces lilacinus on a slant culture medium under an aseptic condition, and culturing for 40 hours at 36 ℃; the slant culture medium is as follows: 12g of glucose, 6g of peptone, 4g of beef extract, 2g of sodium chloride, 16g of agar powder and 1000mL of water, wherein the pH value is 7.0;
b. shake cultivation: inoculating the strain cultured in the step a into a shake flask culture medium under the aseptic condition, and carrying out shake culture for 16 hours at 36 ℃ under the condition of 180-200 r/min; the shake flask culture medium is as follows: 12g of glucose, 6g of peptone, 4g of beef extract, 2g of yeast extract, 2.5g of monopotassium phosphate, 5g of magnesium sulfate and 1000mL of water, wherein the pH value is 7.0-7.5;
c. culturing in a fermentation tank: b, inoculating the strain cultured in the step b into a fermentation medium under the aseptic condition, carrying out fermentation culture for 40 hours under the conditions of 0.5kg of tank pressure, 36 ℃ of temperature, 1:0.9 of ventilation capacity and 190r/min of rotation speed, and taking out of the tank when 80% of bacteria become spores to obtain fermentation liquor; the fermentation medium is as follows: 42g of cane sugar, 23g of soybean meal, 13g of peptone, 5g of ammonium sulfate, 6g of fish meal, 5g of magnesium sulfate, 3g of manganese sulfate, 2.5g of monopotassium phosphate, 1.5g of calcium carbonate and 1000mL of water, wherein the pH value is 7.5;
d. and c, concentrating the fermentation liquor obtained in the step c, and drying to prepare spore powder to obtain the spore powder with the effective viable count of 100 hundred million cfu/g, namely respectively obtaining the bacillus subtilis microbial inoculum, the rhodopseudomonas palustris microbial inoculum, the trichoderma harzianum microbial inoculum and the paecilomyces lilacinus microbial inoculum.
Claims (6)
1. A planting method of paeonia rockii is characterized in that: the method comprises the following steps:
1) preparation before planting:
3 months before planting, deeply ploughing the soil to a depth of 30-40 cm, insolating for 3-4 days in the sun, applying a base fertilizer to the soil according to 800 kg/mu, and applying a soil conditioner to the soil according to 1 kg/mu, wherein the base fertilizer is corn straw with a water content of 50-60% after crushing, wheat bran and fresh walnut green husk after crushing according to a mass ratio of 35: 50: 25 mixing, placing in nitrogen, fermenting at 45 deg.C for 2 days, at 55 deg.C for 2 days, and at 60 deg.C for 3 days; after fertilizing for 10 days, spraying magnetized water to the soil according to 300 kg/mu, after spraying for 2 days, paving buckwheat bran with the thickness of 2cm and wheat bran with the thickness of 1cm on the surface of the soil in sequence, wherein the water content of the buckwheat bran is 35-50%, the water content of the wheat bran is 30-33%, after spraying for 5 days, applying a compound microbial agent, wherein the compound microbial agent consists of a bacillus subtilis microbial agent, a rhodopseudomonas palustris microbial agent, a trichoderma harzianum microbial agent and a paecilomyces lilacinus microbial agent, the mass ratio of the four microbial agents is 2:2:1:2, and the total effective viable count of the compound microbial agent is 100 cfu/g;
2) seedling culture:
putting pretreated paeonia rockii seeds into a seedling raising tray, wherein a sandstone layer with the thickness of 1.5cm, a sponge water absorption layer with the thickness of 1cm and a black soil layer with the thickness of 3cm are sequentially paved in the seedling raising tray from bottom to top, and the sponge water absorption layer is composed of a sponge layer with the thickness of 0.3 cm; keeping the room temperature at 25-30 ℃, and culturing for 15 days after the seeds germinate to obtain paeonia rockii seedlings; wherein the pretreatment specifically comprises the following steps: putting Paeonia suffruticosa seeds into clear water, treating for 30min in ultrasonic wave with frequency of 30KHz, draining, putting Paeonia suffruticosa seeds into 10% ammonium nitrate solution by mass percent, soaking for 3h, and irradiating for 3min in He-Ne laser with wavelength of 632.8 nm;
3) planting and managing: according to 9 strains/m2Planting the paeonia rockii seedlings in soil, watering the paeonia rockii seedlings once at 6 o' clock each day within 10 days after planting, and applying nutrient solution to roots of the paeonia rockii seedlings on 2 nd day after planting, wherein the nutrient solution comprises ammonium nitrate, potassium nitrate and purified water in a mass ratio of 19:20: 500.
2. The paeonia rockii planting method of claim 1, wherein: the magnetized water is obtained by magnetizing deep ocean water in a magnetic field with the magnetic field intensity of 0.3T.
3. The paeonia rockii planting method of claim 1, wherein: in the step 3), white sugar powder is applied to the roots of the paeonia rockii seedlings according to the adding amount of 40g of each paeonia rockii seedling.
4. The paeonia rockii planting method of claim 1, wherein: the buckwheat bran in the step 1) is buckwheat bran fermented at 40 ℃.
5. The paeonia rockii planting method of claim 1, wherein: in the step 1), after deep ploughing, the starch grafted acrylate is put into the soil according to 100 kg/mu.
6. The paeonia rockii planting method of claim 1, wherein: in the step 1), the preparation method of the compound microbial agent is characterized by comprising the following steps:
(1) preparation of a Single microbial inoculum
a. Slant culture: respectively inoculating bacillus subtilis, rhodopseudomonas palustris, trichoderma harzianum and paecilomyces lilacinus on a slant culture medium under an aseptic condition, and culturing for 36-48 hours at 35-37 ℃; the slant culture medium is as follows: 10-15g of glucose, 5-7g of peptone, 3-5g of beef extract, 2g of sodium chloride, 16g of agar powder and 1000mL of water, wherein the pH value is 7.0-7.5;
b. shake cultivation: inoculating the strain cultured in the step a into a shake flask culture medium under the aseptic condition, and carrying out shake culture for 12-18 hours at 35-37 ℃ under the condition of 180-200 r/min; the shake flask culture medium is as follows: 10-15g of glucose, 5-7g of peptone, 3-5g of beef extract, 1-3g of yeast extract, 2.5g of monopotassium phosphate, 5g of magnesium sulfate and 1000mL of water, wherein the pH value is 7.0-7.5;
c. culturing in a fermentation tank: b, inoculating the strain cultured in the step b into a fermentation medium under the aseptic condition, carrying out fermentation culture for 36-48 hours under the conditions of 0.5kg of tank pressure, 35-37 ℃ of temperature, 1:0.8-1.0 of ventilation capacity and 180-200r/min of rotation speed, and taking out of the tank when 80% of bacteria become spores to obtain fermentation liquor; the fermentation medium is as follows: 30-45g of sucrose, 20-25g of soybean meal, 10-15g of peptone, 5g of ammonium sulfate, 5-7g of fish meal, 5g of magnesium sulfate, 3g of manganese sulfate, 2.5g of monopotassium phosphate, 1.5g of calcium carbonate and 1000mL of water, wherein the pH value is 7.5-8.0;
d. and c, concentrating the fermentation liquor obtained in the step c, and drying to prepare spore powder to obtain the spore powder with the effective viable count of 100 hundred million cfu/g, namely respectively obtaining the bacillus subtilis microbial inoculum, the rhodopseudomonas palustris microbial inoculum, the trichoderma harzianum microbial inoculum and the paecilomyces lilacinus microbial inoculum.
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