CN111172133A

CN111172133A - 一种碱基编辑工具及其用途

Info

Publication number: CN111172133A
Application number: CN202010163058.3A
Authority: CN
Inventors: 刘亚京; 黄师圣; 黄行许
Original assignee: ShanghaiTech University
Current assignee: ShanghaiTech University
Priority date: 2020-03-10
Filing date: 2020-03-10
Publication date: 2020-05-19
Anticipated expiration: 2040-03-10
Also published as: CN114058604B; CN114058604A; CN111172133B

Abstract

本发明涉及生物技术领域，特别是涉及一种碱基编辑工具及其用途。本发明提供一种融合蛋白，所述融合蛋白自N端至C端依次包括第一nCas9片段、嵌合插入片段、第二nCas9片段和两个UGI片段，所述嵌合插入片段选自APOBEC1片段或APOBEC3A片段。本发明提供了一种新的碱基编辑工具，其通过在nCas9上可嵌合位点能够兼容多种脱氨酶的嵌入，相较于nCas9末端融合碱基编辑器，在维持特定的靶向碱基编辑效率的同时，在DNA和RNA上的脱靶情况都极大降低，具有更高的特异性，具有良好的产业化前景。

Description

一种碱基编辑工具及其用途

技术领域

本发明涉及生物技术领域，特别是涉及一种碱基编辑工具及其用途。

背景技术

自2013年CRISPR/Cas9被发表于应用真核生物细胞中的基因编辑。此后，基于CRISPR/Cas9系统的基因编辑技术得到了极大的发展。该系统仅由两个部分组成：负责定位靶位点序列的引导RNA(guide RNA，gRNA)和核酸内切酶Cas9蛋白。二者配合能够高效特异的靶向切割目的位点，造成DNA双链断裂(Double Strain Break,BSD)，从而允许人们利用细胞自身的非同源重组修复(Non-homologous end joining,NHEJ)途径产生DNA片段的缺失或引起移码突变，从而造成基因的敲除。人们也可以利用细胞的同源重组修复(Homologydirected repair,HDR)途径对靶位点进行精确的DNA片段替换或者敲入。

随着CRISPR系统的研究逐渐深入，研究人员发现基于DNA双链断裂的基因编辑存在种种问题。首先，就是编辑产物的不可控性。细胞对DNA上DSB位点进行的NHEJ途径修复产物是随机的，有时只会产生极小片段丢失且没有造成移码突变，因此虽然能够产生DSB，但依然不能保证高效的敲除效率。其次，基于HDR修复途径的编辑效率始终不高，很难在体实现高效的基因编辑效率。最后，CRISPR/Cas9系统的脱靶效应也会导致在编辑过程中对基因组上其他位点上产生不可回复的序列改变。人类绝大多数的遗传疾病都是单碱基突变导致。因此，针对以上问题，开发能够精确编辑单碱基的技术将为能为基础研究和临床疾病治疗提供极大助益。

2017年，哈佛大学David R Liu实验室在Nature上报道了一种基于Cas9的单碱基编辑(base editing，BE)工具。该系统利用nCas9和APOBEC1以及UGI融合能够高效实现胞嘧啶C到胸腺嘧啶T的定向单碱基编辑。单碱基编辑技术一经发表便得到了广泛的关注和应用，研究人员在不同的细胞系以及植物和动物中都实现了高效的编辑。

随着剪辑编辑技术的广泛应用，科研人员也一直开发更精确和灵敏的脱靶检测技术对BE进行更加高要求的检验。2019年，杨辉和高彩霞实验室分别独立在Science上报道了CBE会产生非gRNA依赖的DNA脱靶情况。在培养细胞系中，每个细胞内产生的随机脱靶都不同，这些脱靶位点在细胞群体中会被稀释，从而无法检测。杨辉团队开发了一种更敏感的无偏差脱靶检测方法GOTI来检测BE3的脱靶情况，巧妙利用小鼠胚胎发育放大脱靶位点，便于检测。由于DNA上的随机脱靶无法预测并无法恢复，因此这一脱靶现象也引起了大家对CBE在临床治疗应用上的担忧。同年，Keith Joung实验室和杨辉实验室先后在Nature上报道了CBE在转录组上的严重脱靶情况，BE3能诱导原癌基因和抑癌基因等基因上的数百个基因突变，还可能引起其他严重危害健康的突变。真核细胞中RNA虽然不会被遗传，但是理论上所有RNA都会以表达蛋白或者直接参与细胞功能的调控，因此，脱靶突变的产生也会对细胞产生直接影响。

通过对脱氨酶进行氨基酸突变，能够部分消除BE在RNA上的脱靶编辑。但是，这种方法无法完全保证成功，消除脱靶编辑的同时，可能也会损失靶向编辑效率。此外，针对每种脱氨酶，都需要从头进化并验证，因此该种方法的工作量也巨大。最后BE3在DNA上造成的随机脱靶问题仍是个问题。因此，亟待开发一个通用、方便、低成本的进化技术或者策略对降低BE造成的RNA和DNA脱靶。

发明内容

鉴于以上所述现有技术的缺点，本发明的目的在于提供一种碱基编辑工具及其用途，用于解决现有技术中的问题。

为实现上述目的及其他相关目的，本发明一方面提供一种融合蛋白，所述融合蛋白自N端至C端依次包括第一nCas9片段、嵌合插入片段、第二nCas9片段和两个UGI片段，所述嵌合插入片段选自APOBEC1片段或APOBEC3A片段。

在本发明一些实施方式中，所述第一nCas9片段的氨基酸序列包括：

a)如SEQ ID NO.1所示的氨基酸序列；或，

b)与SEQ ID NO.1具有80％以上序列相似性、且具有a)所限定的氨基酸序列的功能的氨基酸序列，优选为具有nCas9靶向活性；

和/或，所述第二nCas9片段的氨基酸序列包括：

c)如SEQ ID NO.2所示的氨基酸序列；或，

d)与SEQ ID NO.2具有80％以上序列相似性、且具有e)所限定的氨基酸序列的功能的氨基酸序列，优选为具有nCas9靶向活性。

在本发明一些实施方式中，所述APOBEC1片段的氨基酸序列包括：

e)如SEQ ID NO.3所示的氨基酸序列；或，

f)与SEQ ID NO.3具有80％以上序列相似性的氨基酸序列、且具有a)所限定的氨基酸序列的功能，优选为具有胞嘧啶脱氨酶活性。

在本发明一些实施方式中，所述APOBEC3A片段的氨基酸序列包括：

i)如SEQ ID NO.4所示的氨基酸序列；或，

j)与SEQ ID NO.4具有80％以上序列相似性的氨基酸序列、且具有c)所限定的氨基酸序列的功能，优选为具有胞嘧啶脱氨酶活性。

在本发明一些实施方式中，所述UGI片段的氨基酸序列包括：

k)如SEQ ID NO.5所示的氨基酸序列；或，

l)与SEQ ID NO.5具有80％以上序列相似性的氨基酸序列、且具有c)所限定的氨基酸序列的功能，优选为具有抑制尿嘧啶DNA糖基化活性。

在本发明一些实施方式中，所述融合蛋白还包括核定位信号片段，优选的，所述核定位信号片段的氨基酸序列包括SEQ ID NO.6所示的氨基酸序列。

在本发明一些实施方式中，所述融合蛋白还包括柔性连接肽片段，优选的，所述柔性连接肽片段的氨基酸序列包括SEQ ID NO.7或SEQ ID NO.8所示的氨基酸序列。

在本发明一些实施方式中，所述融合蛋白的氨基酸序列如SEQ ID No.9～10其中之一所示。

本发明另一方面提供一种分离的多核苷酸，编码上述的融合蛋白。

本发明另一方面提供一种构建体，所述构建体含有上述的分离的多核苷酸。

本发明另一方面提供一种表达系统，所述表达系统含有上述的构建体或基因组中整合有外源的上述的多核苷酸。

在本发明一些实施方式中，所述表达系统的宿主细胞选自真核细胞或原核细胞，优选选自小鼠细胞、人细胞，更优选选自小鼠脑神经瘤细胞、人胚胎肾细胞、或人宫颈癌细胞、人结肠癌细胞、人骨肉瘤细胞，更优选选自N2a细胞、HEK293FT细胞、Hela细胞、HCT116细胞、或U2OS细胞。

本发明另一方面提供上述的融合蛋白、上述的分离的多核苷酸、上述的构建体或上述的表达系统在基因编辑中的用途。

在本发明一些实施方式中，所述用途具体为在真核生物的基因编辑中的用途。

本发明另一方面提供一种碱基编辑体系，包括上述的融合蛋白，所述碱基编辑体系还包括sgRNA。

本发明另一方面提供一种基因编辑方法，包括：通过上述的融合蛋白、或上述的碱基编辑体系进行基因编辑。

附图说明

图1显示为本发明基于Mu转座酶构建nCas9随机插入文库的示意图。

图2显示为本发明筛选得到的nCas9有效插入位点和碱基编辑效率结果示意图。

图3显示为本发明比较SpCas9的同源物的不保守区域的结果示意图。

图4显示为本发明筛选得到的CE-ABE在人细胞基因组上的碱基编辑结果示意图。

图5显示为本发明CE-ABE在预测RNA位点上脱靶编辑结果示意图。

图6显示为本发明CE-ABE在转录组水平产生的脱靶编辑结果示意图。

图7显示为本发明CE-ABE在脱靶检测样本中的靶向编辑效率结果示意图。

图8显示为本发明CE-ABE^1048-1063和ABEmax在293T细胞中具有相当的编辑效率结果示意图。

图9显示为本发明CE-ABE^1048-1063和ABEmax在N2a细胞中具有相当的编辑效率结果示意图。

图10显示为本发明CE-BE^1048-1063和AncBE4max在293T细胞中具有相当的编辑效率结果示意图。

图11显示为本发明CE-A3A^1048-1063和BE-A3A在293T细胞中具有相当的编辑效率结果示意图。

图12显示为本发明CE-BE和AncBE4max在293T细胞中RNA上的脱靶编辑情况示意图。

图13显示为本发明CE-A3A和BE-A3A在293T细胞中RNA上的脱靶编辑情况示意图。

图14显示为本发明BE4max、BE-A3A、CE-BE^1048-1063和CE-A3A^1048-1063在DNA上的靶向编辑结果示意图。

图15显示为本发明BE4max、CE-BE^1048-1063(CE-BE4max)在DNA上的脱靶编辑结果示意图。

图16显示为本发明BE-A3A和CE-A3A^1048-1063(CE-A3A)在DNA上的脱靶编辑结果示意图。

具体实施方式

本发明发明人经过大量探索性研究，发现在nCas9蛋白内部的合适位置嵌合融合功能片段，能够极大地同时降低BE在RNA和DNA上的脱靶情况，并且不影响BE的靶向编辑效率，在此基础上完成了本发明。

本发明第一方面提供一种融合蛋白，所述融合蛋白自N端至C端依次包括第一nCas9片段、嵌合插入片段、第二nCas9片段和两个UGI片段，所述嵌合插入片段选自APOBEC1片段或APOBEC3A片段。所述融合蛋白是将nCas9(GenBank:MK048158.1)的1048Thr-1063Ile片段替换为嵌合插入片段，在sgRNA的引导下在靶向位点做碱基编辑，能够极大地同时降低BE在RNA和DNA上的脱靶情况，并且不影响BE的靶向编辑效率。

本发明所提供的融合蛋白中，所述第一nCas9片段的氨基酸序列可以包括：a)如SEQ ID NO.1所示的氨基酸序列；或，b)与SEQ ID NO.1具有80％以上序列相似性的氨基酸序列、且具有a)所限定的氨基酸序列的功能。具体的，所述b)中的氨基酸序列具体指：如SEQID No.1所示的氨基酸序列经过取代、缺失或者添加一个或多个(具体可以是1-50、1-30个、1-20个、1-10个、1-5个、1-3个、1个、2个、或3个)氨基酸而得到的，或者在N-末端和/或C-末端添加一个或多个(具体可以是1-50个、1-30个、1-20个、1-10个、1-5个、1-3个、1个、2个、或3个)氨基酸而得到的，且具有氨基酸如SEQ ID No.1所示的多肽片段的功能的多肽片段，例如，可以是nCas9第一片段与nCas9第二片段配合后依然具有nCas9的靶向活性，更具体可以是能够在合适的gRNA的引导下靶向DNA的活性。所述b)中的氨基酸序列可与SEQ ID No.1具有80％、85％、90％、93％、95％、97％、或99％以上的相似性。所述第一nCas9片段通常源自大肠杆菌。

本申请中，序列的相似性(sequence identity)通常指参与对比的序列中相同的氨基酸残基的百分比，可采用本领域公知的计算软件计算两条或多条目的序列的相似性，例如，可以是来自NCBI的软件。

本发明所提供的融合蛋白中，所述第二nCas9片段的氨基酸序列可以包括：c)如SEQ ID NO.2所示的氨基酸序列；或，d)与SEQ ID NO.2具有80％以上序列相似性的氨基酸序列、且具有c)所限定的氨基酸序列的功能。具体的，所述d)中的氨基酸序列具体指：如SEQID No.2所示的氨基酸序列经过取代、缺失或者添加一个或多个(具体可以是1-50、1-30个、1-20个、1-10个、1-5个、1-3个、1个、2个、或3个)氨基酸而得到的，或者在N-末端和/或C-末端添加一个或多个(具体可以是1-50个、1-30个、1-20个、1-10个、1-5个、1-3个、1个、2个、或3个)氨基酸而得到的，且具有氨基酸如SEQ ID No.2所示的多肽片段的功能的多肽片段，例如，可以是nCas9第一片段与nCas9第二片段配合后依然具有nCas9的靶向活性，更具体可以是能够在合适的gRNA的引导下靶向DNA的活性。所述d)中的氨基酸序列可与SEQ ID No.2具有80％、85％、90％、93％、95％、97％、或99％以上的相似性。所述第二nCas9片段通常源自大肠杆菌(Streptococcus pyogenes)。

本发明所提供的融合蛋白中，所述APOBEC1片段的氨基酸序列可以包括：e)如SEQID NO.3所示的氨基酸序列；或，f)与SEQ ID NO.3具有80％以上序列相似性的氨基酸序列、且具有e)所限定的氨基酸序列的功能。具体的，所述f)中的氨基酸序列具体指：如SEQ IDNo.3所示的氨基酸序列经过取代、缺失或者添加一个或多个(具体可以是1-50、1-30个、1-20个、1-10个、1-5个、1-3个、1个、2个、或3个)氨基酸而得到的，或者在N-末端和/或C-末端添加一个或多个(具体可以是1-50个、1-30个、1-20个、1-10个、1-5个、1-3个、1个、2个、或3个)氨基酸而得到的，且具有氨基酸如SEQ ID No.3所示的多肽片段的功能的多肽片段，例如，可以是具有胞嘧啶脱氨酶活性，更具体可以是将胞嘧啶(cytosine，C)脱氨产生尿嘧啶(uracil，U)的功能。所述f)中的氨基酸序列可与SEQ ID No.3具有80％、85％、90％、93％、95％、97％、或99％以上的相似性。所述APOBEC1片段通常源自大鼠(rat)。

本发明所提供的融合蛋白中，所述APOBEC3A片段的氨基酸序列可以包括：g)如SEQID NO.4所示的氨基酸序列；或，h)与SEQ ID NO.4具有80％以上序列相似性的氨基酸序列、且具有g)所限定的氨基酸序列的功能。具体的，所述h)中的氨基酸序列具体指：如SEQ IDNo.4所示的氨基酸序列经过取代、缺失或者添加一个或多个(具体可以是1-50、1-30个、1-20个、1-10个、1-5个、1-3个、1个、2个、或3个)氨基酸而得到的，或者在N-末端和/或C-末端添加一个或多个(具体可以是1-50个、1-30个、1-20个、1-10个、1-5个、1-3个、1个、2个、或3个)氨基酸而得到的，且具有氨基酸如SEQ ID No.4所示的多肽片段的功能的多肽片段，例如，可以是具有胞嘧啶脱氨酶活性，更具体可以是将胞嘧啶(cytosine，C)脱氨产生尿嘧啶(uracil，U)的功能。所述h)中的氨基酸序列可与SEQ ID No.4具有80％、85％、90％、93％、95％、97％、或99％以上的相似性。所述APOBEC3A片段通常源自人。

本发明所提供的融合蛋白中，可以包括两个独立的UGI片段，这两个UGI片段的氨基酸序列可以各自独立地包括：i)如SEQ ID NO.5所示的氨基酸序列；或，j)与SEQ ID NO.5具有80％以上序列相似性的氨基酸序列、且具有i)所限定的氨基酸序列的功能。具体的，所述j)中的氨基酸序列具体指：如SEQ ID No.5所示的氨基酸序列经过取代、缺失或者添加一个或多个(具体可以是1-50、1-30个、1-20个、1-10个、1-5个、1-3个、1个、2个、或3个)氨基酸而得到的，或者在N-末端和/或C-末端添加一个或多个(具体可以是1-50个、1-30个、1-20个、1-10个、1-5个、1-3个、1个、2个、或3个)氨基酸而得到的，且具有氨基酸如SEQ ID No.5所示的多肽片段的功能的多肽片段，例如，可以是具有抑制尿嘧啶DNA糖基化反应的功能。所述j)中的氨基酸序列可与SEQ ID No.5具有80％、85％、90％、93％、95％、97％、或99％以上的相似性。所述UGI片段通常来自枯草芽孢杆菌噬菌体(Bacillus subtilisbacteriophage)。

本发明所提供的融合蛋白中，所述的取代、缺失或者添加可以是保守氨基酸取代。所述“保守氨基酸取代”具体可以是指氨基酸残基被其他具有相似侧链的氨基酸残基取代的情况。具有相似侧链的氨基酸残基家族对于本领域技术人员来说应该是已知的，例如，可以是包括但不限于碱性侧链(例如赖氨酸，精氨酸，组氨酸)，酸性侧链(例如天冬氨酸，谷氨酸)，不带电荷的极性侧链(例如，甘氨酸，天冬酰胺，谷氨酰胺，丝氨酸，苏氨酸，酪氨酸，半胱氨酸)，非极性侧链(例如丙氨酸，缬氨酸，亮氨酸，异亮氨酸，脯氨酸，苯丙氨酸，甲硫氨酸，色氨酸)异亮氨酸)和芳族侧链(例如酪氨酸，苯丙氨酸，色氨酸，组氨酸)等家族。保守型氨基酸取代更具体可以包括但不限于下表中所列的具体情况，表1(氨基酸相似度矩阵)中的数字表示两个氨基酸之间的相似度，当数字大于等于0时认为是保守氨基酸取代，表2为示例性的保守氨基酸取代的方案。

表1

	C	G	P	S	A	T	D	E	N	Q	H	K	R	V	M	I	L	F	Y	W
																					W	-8	-7	-6	-2	-6	-5	-7	-7	-4	-5	-3	-3	2	-6	-4	-5	-2	0	0	17
Y	0	-5	-5	-3	-3	-3	-4	-4	-2	-4	0	-4	-5	-2	-2	-1	-1	7	10
																					F	-4	-5	-5	-3	-4	-3	-6	-5	-4	-5	-2	-5	-4	-1	0	1	2	9
L	-6	-4	-3	-3	-2	-2	-4	-3	-3	-2	-2	-3	-3	2	4	2	6
																					I	-2	-3	-2	-1	-1	0	-2	-2	-2	-2	-2	-2	-2	4	2	5
M	-5	-3	-2	-2	-1	-1	-3	-2	0	-1	-2	0	0	2	6
																					V	-2	-1	-1	-1	0	0	-2	-2	-2	-2	-2	-2	-2	4
R	-4	-3	0	0	-2	-1	-1	-1	0	1	2	3	6
																					K	-5	-2	-1	0	-1	0	0	0	1	1	0	5
H	-3	-2	0	-1	-1	-1	1	1	2	3	6
																					Q	-5	-1	0	-1	0	-1	2	2	1	4
N	-4	0	-1	1	0	0	2	1	2
																					E	-5	0	-1	0	0	0	3	4
D	-5	1	-1	0	0	0	4
																					T	-2	0	0	1	1	3
A	-2	1	1	1	2
																					S	0	1	1	1
P	-3	-1	6
																					G	-3	5
C	12

表2

本发明所提供的融合蛋白中，所述融合蛋白还可以包括核定位信号片段(BPNLS片段)，所述核定位信号片段通常可以与入核载体相互作用，从而可以使蛋白能够被运送进细胞核。所述核定位信号片段可以位于nCas9第一段片段的N端以及两个UGI片段中第二个UGI的C端，即整个融合蛋白两端各一个BPNLS片段。所述BPNLS片段的氨基酸序列可以包括SEQID NO.6所示的氨基酸序列。

本发明所提供的融合蛋白中，所述融合蛋白还可以包括柔性连接肽片段。所述柔性连接肽片段通常是成柔软线性的一类易弯折的氨基酸片段，通常可以使连接的两个蛋白之间有一定的活动空间。例如，柔性连接肽片段可以是XTEN肽段等。所述柔性连接肽片段(例如，XTEN肽段)可以位于nCas9第一个片段和嵌入片段(ABOBEC1或APOBEC3A)之间，也可以位于嵌入片段(ABOBEC1或APOBEC3A)和nCas9第二个片段之间。所述XTEN肽段的氨基酸序列可以包括SEQ ID NO.7所示的氨基酸序列。再例如，柔性连接肽片段可以是GS肽段等。所述柔性连接肽片段(例如，GS肽段)可以位于nCas9第二个片段与两个UGI片段中第一个UGI之间，也可以位于两个UGI之间。所述柔性连接肽片段的氨基酸序列可以包括SEQ ID NO.8所示的氨基酸序列。

本发明所提供的融合蛋白中，所述融合蛋白自N端至C端可以依次包括BPNLS肽段、第一nCas9片段、XTEN肽段、APOBEC1、XTEN肽段、第二nCas9片段、GS肽段和两个UGI片段。在本发明一具体实施例中，所述融合蛋白自N端至C端可以依次包括BPNLS肽段、第一nCas9片段、XTEN肽段、APOBEC1、XTEN肽段、第二nCas9片段、GS肽段和两个UGI片段，所述融合蛋白的氨基酸序列如SEQ ID No.9所示。

本发明所提供的融合蛋白中，所述融合蛋白自N端至C端可以依次包括BPNLS肽段、第一nCas9片段、XTEN肽段、APOBEC3A、XTEN肽段、第二nCas9片段、GS肽段和两个UGI片段。在本发明一具体实施例中，所述融合蛋白自N端至C端可以依次包括BPNLS肽段、第一nCas9片段、XTEN肽段、APOBEC3A、XTEN肽段、第二nCas9片段、GS肽段和两个UGI片段，所述融合蛋白的氨基酸序列如SEQ ID No.10所示。

本发明第二方面提供一种分离的多核苷酸，编码本发明第一方面所提供的融合蛋白。

本发明第三方面提供一种构建体，所述构建体含有本发明第二方面所提供的分离的多核苷酸。所述构建体通常可以通过将所述分离的多核苷酸插入合适的表达载体中构建获得，本领域技术人员可选择合适的表达载体，例如，所述表达载体可以是包括但不限于pCMV表达载体、pSV2表达载体等。

本发明第四方面提供一种表达系统，所述表达系统含有本发明第三方面所提供的构建体或基因组中整合有外源的本发明第二方面所提供的分离的多核苷酸。所述表达系统可以是宿主细胞，所述宿主细胞可以表达如上所述的融合蛋白，所述融合蛋白可以与sgRNA相配合，从而可以将所述融合蛋白定位到目标区域，实现目标区域的碱基编辑。在本发明另一具体实施例中，所述宿主细胞可以是真核细胞和/或原核细胞，更具体可以是小鼠细胞、人细胞等，更具体可以是小鼠脑神经瘤细胞、人胚胎肾细胞、人宫颈癌细胞、人结肠癌细胞、人骨肉瘤细胞等，更具体可以是N2a细胞、HEK293FT细胞、Hela细胞、HCT116细胞、或U2OS细胞等。

本发明第五方面提供本发明第一方面所提供的融合蛋白、或本发明第二方面所提供的分离的多核苷酸、或本发明第三方面所提供的构建体、或本发明第四方面所提供的表达系统在基因编辑中的用途，优选为真核生物的基因编辑中的用途，所述真核生物具体可以是后生动物，具体可以是包括但不限于人、小鼠等。所述用途具体可以是包括但不限于由C到T的碱基编辑等，这些碱基编辑可以应用于编辑剪接受体/供体位点来调节RNA剪接，也可以用于进行模型(例如，疾病模型、细胞模型、动物模型等)的构建或人类疾病的治疗等。在本发明一具体实施例中，被编辑的对象可以是胚胎、细胞等。

本发明第六方面提供一种碱基编辑体系，包括本发明第一方面所提供的融合蛋白，所述碱基编辑体系还包括sgRNA。本领域技术人员可以根据基因的目标编辑区域，选择合适的靶向特异性位点的sgRNA。例如，所述sgRNA的序列通常可以与目标区域至少部分互补，从而可以与所述融合蛋白相配合，将所述融合蛋白定位到目标区域，实现靶点区域内的碱基编辑，例如，可以是胞嘧啶脱氨基反应，即将胞嘧啶(cytosin，C)脱氨产生胸腺嘧啶(thymine，T)。

本发明第七方面提供一种碱基编辑方法，包括：通过本发明第一方面所提供的融合蛋白、或本发明第六方面所提供的碱基编辑体系进行基因编辑。例如，所述基因编辑方法可以包括：在适当条件下培养本发明第四方面所提供的表达系统，从而表达所述融合蛋白，所述融合蛋白可以在与其配合的靶向目标区域的sgRNA存在的条件下，对靶标区域进行碱基编辑。提供所述sgRNA存在的条件的方法对于本领域技术人员来说应该是已知的，例如，可以是在适当条件下培养能够表达所述sgRNA的表达系统，所述表达系统可以是包括含有编码所述sgRNA的多核苷酸的表达载体的宿主细胞、或染色体中整合有编码所述sgRNA的多核苷酸的宿主细胞。在本发明一具体实施例中，所述sgRNA与所述融合蛋白可以在同一宿主细胞中表达，所述宿主细胞可以为靶细胞。在本发明另一具体实施例中，所述基因编辑为体外基因编辑。

本发明提供了一种新的碱基编辑工具，其通过在nCas9上可嵌合位点能够兼容多种脱氨酶的嵌入，相较于nCas9末端融合碱基编辑器，在维持特定的靶向碱基编辑效率的同时，在DNA和RNA上的脱靶情况都极大降低，具有更高的特异性，具有良好的产业化前景。

以下通过特定的具体实例说明本发明的实施方式，本领域技术人员可由本说明书所揭露的内容轻易地了解本发明的其他优点与功效。本发明还可以通过另外不同的具体实施方式加以实施或应用，本说明书中的各项细节也可以基于不同观点与应用，在没有背离本发明的精神下进行各种修饰或改变。

在进一步描述本发明具体实施方式之前，应理解，本发明的保护范围不局限于下述特定的具体实施方案；还应当理解，本发明实施例中使用的术语是为了描述特定的具体实施方案，而不是为了限制本发明的保护范围；在本发明说明书和权利要求书中，除非文中另外明确指出，单数形式“一个”、“一”和“这个”包括复数形式。

当实施例给出数值范围时，应理解，除非本发明另有说明，每个数值范围的两个端点以及两个端点之间任何一个数值均可选用。除非另外定义，本发明中使用的所有技术和科学术语与本技术领域技术人员通常理解的意义相同。除实施例中使用的具体方法、设备、材料外，根据本技术领域的技术人员对现有技术的掌握及本发明的记载，还可以使用与本发明实施例中所述的方法、设备、材料相似或等同的现有技术的任何方法、设备和材料来实现本发明。

除非另外说明，本发明中所公开的实验方法、检测方法、制备方法均采用本技术领域常规的分子生物学、生物化学、染色质结构和分析、分析化学、细胞培养、重组DNA技术及相关领域的常规技术。这些技术在现有文献中已有完善说明，具体可参见Sambrook等MOLECULAR CLONING：A LABORATORY MANUAL，Second edition，Cold Spring HarborLaboratory Press，1989 and Third edition，2001；Ausubel等，CURRENT PROTOCOLS INMOLECULAR BIOLOGY，John Wiley&Sons，New York，1987 and periodic updates；theseries METHODS IN ENZYMOLOGY，Academic Press，San Diego；Wolffe，CHROMATINSTRUCTURE AND FUNCTION，Third edition，Academic Press，San Diego，1998；METHODS INENZYMOLOGY，Vol.304，Chromatin(P.M.Wassarman and A.P.Wolffe，eds.)，AcademicPress，San Diego，1999；和METHODS IN MOLECULAR BIOLOGY，Vol.119，ChromatinProtocols(P.B.Becker，ed.)Humana Press，Totowa，1999等。

实施例1

1、基于MuA转座酶的TadA-TadA*转座子的构建

由上海铂尚生物科技有限公司合成TadA-TadA*转座子序列(SEQ ID NO.11)，使用诺唯赞公司的高保真酶试剂盒(Vazyme,P501-d2)进行PCR扩增。正向引物：GGTCTCTGATCCGGCGCACGAA(SEQ ID NO.71)，反相引物：GGTCTCTGATCCGGCGCACGAA(SEQ IDNO.72)；

扩增体系如下：

表3

PCR条件如下：

表4

PCR扩增产物经通过AxyPrep PCR Clean-up试剂盒(Axygen,AP-PCR-500G)纯化回收，待用。

2、sgRNA的构建

检测ABE(Adenine base editing)在真核细胞中靶向编辑效率所用sgRNA为ABE-site1。后续检测ABE和CE-ABE(centrally encapsulate ABE)在HEK293T细胞的8个内源基因位点sgRNA为site2-site9。后续检测ABE和CE-ABE在N2a细胞的12个内源基因位点sgRNA为site10-site21。位点序列如SEQ ID NO.12～32所示。检测CE-CBE和CE-A3A所用sgRNA均为靶向HEK293T细胞的内源基因位点，sgRNA为site22-site32。位点序列如SEQ ID NO.57～67所示。根据靶向位点序列设计20个碱基互补配对的上下游引物，加灭菌水溶解至100μM。经退火后连接到pGL3-U6-sgRNA(Addgene#51133)载体上，以构建靶向特异性sgRNA。

退火体系如下所示：

表5

正向引物	4.5μL
		反向引物	4.5μL
10×NEB buffer2	1μL

退火程序如下：

表6

95℃	5min
		95-85℃	-2℃/s
85-25℃	-0.1℃/s
		4℃	∞

利用BsaI(NEB,R0535S)对pGL3-U6-sgRNA(Addgene#51133)质粒进行酶切以得到线性化sgRNA载体。酶切体系如下所示：

表7

水	补水至50μL
		PGL3-U6质粒	10μg
10×cutsmart buffer	5μL
		BsaI酶	5μL

以上反应体系配置好后，置于37℃条件下反应5h，酶切产物用AxyPrep DNA凝胶回收试剂盒(Axygen,AP-GX-250G)做割胶回收得到线性化载体。取50ng线性化载体与3μL退火产物通过T4连接酶(NEB,M0202S)连接，16℃孵育2小时后并转化涂板，经Sanger测序得到正确的靶向特异性sgRNA。连接体系如下：

表8

水	补水至10μL
		PGL3-U6-BsaI酶切线性片段	20ng
退货产物	1μL
		Solution I	5μL

连接产物随后进行转化，复苏30min，涂板于氨卞抗性的LB琼脂平板，37℃培养过夜。挑选单克隆进行测序验证，ABEsite1-site21的sgRNA。

3、用于MuA转座酶随机插入的受体质粒构建

质粒构建所用引物均由上海铂尚生物科技有限公司合成。

首先以pCMV-ABEmax(Addgene，#112095)质粒为模板，正向引物：GACAAGAAGTACAGCATCGGCC(SEQ ID NO.73)，反向引物：GCTGTACTTCTTGTCACTGCTGACTTTCCGCTTCTTC(SEQ ID NO.74)，得到7629bp长度的片段，PCR扩增产物经通过AxyPrep PCRClean-up试剂盒(Axygen,AP-PCR-500G)纯化回收，再使用Gibson Assembly Master Mix重组试剂盒(NEB，E2611S)对该片段进行重组，反应体系如下：

表9

Gibson Assembly Master Mix(2X)	5μl
		7629bp PCR片段	200ng
无菌水	补水至10μl

反应液混合后置于50℃孵育1h，随后进行转化，复苏30min，涂板于氨卞抗性的LB琼脂平板，37℃培养过夜。挑选单克隆进行测序验证，得到pCMV-nCas9质粒(SEQ IDNO.33)。构建成功的质粒(SEQ ID NO.33)采用AxyPrep plasmids miniprep试剂盒(Axygen,AP-MN-P-250G)进行质粒小提。

以SEQ ID NO.33为模板，正向引物：

GAAGAAGCGGAAAGTCGACAAGAAGTACAGCATCGG(SEQ ID NO.75)，反相引物：CTGAGCTAGCTGTCAACGAGCCCCAGCTGGTTCTTT(SEQ ID NO.76)，PCR扩增得到4507bp长度的nCas9片段；

以PET30质粒为模板，正向引物：CTCACTGATTAAGCATTGGTAAGCGCGGAACCCCTATTTGTT(SEQ ID NO.77)，反相引物：CCGTTTCATGGTGGCATGTATATCTCCTTCTTAAAGTTAAACAAAATT(SEQID NO.78)；PCR扩增4620bp长度的KanR片段；

以pGL3-U6-sgRNA质粒为模板，正向引物：GTATAATACTAGTGCTCTTGCCCGGCGTCAATACGTTTTAGAGCTAGAAATAGCAAGTT(SEQ ID NO.79)，反相引物：gttagcagccggatcaaaaaaagcaccgactcgg(SEQ ID NO.80)，进行PCR扩增132bp长度的U6-sgRNA片段，再以U6-sgRNA产物为模板，正向引物：TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGTGCTCTTGCC(SEQ ID NO.81)，反相引物：GTTAGCAGCCGGATCAAAAAAAGCACCGACTCGG(SEQ ID NO.82)，PCR扩增154bp长度的J23119promoter-gRNA片段；

以pCMV-ABEmax(Addgene，#112095)质粒为模板，正向引物：CTTTTCGGGGAAATGTGGGAAATGTGCGCGGAACC(SEQ ID NO.83)，反相引物：CCCGGCGTCAATACGGGATA(SEQ ID NO.84)，PCR进行扩增，得到386bp长度的AmpR-1片段

以pCMV-ABEmax(Addgene，#112095)质粒为模板，正向引物：GTATTGACGCCGGGTAAGAGCAACTCGGTCGCCGC(SEQ ID NO.85)，反向引物：TTACCAATGCTTAATCAGTGAGGCACC(SEQ IDNO.86)，PCR进行扩增，得到620bp长度的AmpR-1片段。

以上PCR所用的都是诺唯赞公司的高保真酶试剂盒(Vazyme,P501-d2)进行，PCR反应体系如下：

表10

水	补加至50μl
		2xbuffer	25μl
dNTP	1μl
		正向引物(10μM)	2μl
反向引物(10μM)	2μl
		高保真酶	1μl
模板	1ng

PCR程序如下：

表11

以上所有PCR扩增产物都经AxyPrep PCR Clean-up试剂盒(Axygen,AP-PCR-500G)纯化回收，再使用Gibson Assembly Master Mix重组试剂盒(NEB，E2611S)对该片段进行重组，反应体系如下：

表12

Gibson Assembly Master Mix(2X)	10μl
		nCas9片段(4507bp)	80ng
KanR片段(4620bp)	80ng
		J23119promoter-gRNA片段(154bp)	10ng
AmpR-1片段(386bp)	20ng
		AmpR-2片段(620bp)	30ng
无菌水	补水至20μl

反应液混合后置于50℃孵育1h，随后进行转化，复苏30min，涂板于卡那抗性的LB琼脂平板，37℃培养过夜。挑选单克隆进行测序验证，得到pET-nCas9-gRNA-AmpR(A118X)-KanR质粒(SEQ ID NO.34)。构建成功的质粒(SEQ ID NO.34)采用AxyPrep plasmidsminiprep试剂盒(Axygen,AP-MN-P-250G)进行质粒小提。

4、体外随机插入文库的构建

PCR得到的TadA-TadA*转座子片段、pET-nCas9-gRNA-AmpR(A118X)-KanR质粒(SEQID NO.34)和MuA转座酶(Thermo Fisher，F-701)在体外反应，形成质粒中随机插入TadA-TadA*转座子片段的插入质粒库，流程具体如图1所示。

具体反应体系如下：

表13

TadA-TadA*片段	250ng
		质粒(SEQ ID NO.34)	500ng
MuA转座酶	1μL
		5×Reaction Buffer for MuA Transposase	4μL
水	补水至20μL

反应液在30℃孵育1小时以实现随机插入，然后在75℃孵育10分钟以灭活MuA转座酶。随后用异丙醇沉淀纯化出DNA，并重悬于5μL去离子水中，然后电转到100μLBL21(DE3)Electro(Shanghai Weidi Biotechnology，EE1002)感受态细胞中。然后加入1ml SOC培养基，并将细菌在37℃下培养1小时。将上述转化后在SOC培养基中复苏1小后的细菌涂布在数个含10μg/mL卡那霉素的LB琼脂板上，并在37℃下孵育16小时。然后刮下来平板上的菌落，然后采用AxyPrep plasmids miniprep试剂盒(Axygen,AP-MN-P-250G)进行质粒小提。提取的MuA随机插入质粒库在中国北京的Novogene生物信息学研究所进行测序，使用IlluminaHiSeq X Ten(2×150PE)对构建的转座子文库进行测序。首先使用默认参数BWA v0.7.16将所有数据读段映射到主链序列。提取断的读段，然后将其映射到插入序列。检查所有映射的读取，并将断点记录为插入位点。最终得到插入文库的随机插入情况，具体在nCas9上的插入位点以氨基酸碳基端计算(如：插入发生在第五位天冬氨酸碳基端，则该插入位点为5)，统计后发现，基于MuA的随机插入文库覆盖度非常高，nCas9上99.99％的氨基酸位点都至少有一次插入发生，将插入频次和插入位点从小到大排列如下：

表14

5、大肠杆菌中筛选功能性嵌入融合ABE蛋白的表达质粒

将上述转化后在SOC培养基中复苏1小后的细菌涂布在数个含10μg/mL卡那霉素的LB琼脂板上，并在37℃下孵育16小时。然后刮下来平板上的菌落，并将其重悬于含有500μMIPTG的100mLLB中。将培养物温育10-12h以诱导nCas9表达并修复AmpR(A118X)上的突变。然后将减少量的细胞(5mL，1mL，500μL，100μL)接种到含有氨苄青霉素(10μg/mL)和卡那霉素(10μg/mL)的15cm LB琼脂平板上。37℃孵育过夜后，挑选菌落并进行Sanger测序，以评估AmpR(A118X)上的碱基编辑并确定TadA-TadA*插入位点。筛选到以下插入位点，具体位置为51，62，63，249，531，584，719，768，770，776，782，790，808，819，831，832，842，893，924，1009，1010，1018，1033，1050，1051，1063，1072，1073，1090，1227，1246，1248，1253，1260，1263，1276，1290，1302，1346，TadA-TadA*片段插入在这些位点的碳端。经过AmpR抗性筛选，以及测序分析AmpR(A118X)位点的修复情况，发现以上位点插入TadA-TadA*能够形成有碱基编辑功能的嵌合融合版本ABE，对应的插入位置和碱基编辑效率如图2所示。

6、大肠杆菌中CE-ABE突变效率的检测

首先，我们在将上述电转随机插入文库的大肠杆菌均匀涂布于含有氨苄抗生素的琼脂糖平板上，培养箱中过夜培养。挑取阳性克隆，分别用引物(cttttcggggaaatgtgggaaatgtgcgcggaacc)(SEQ ID NO.87)和引物(cggatgcctagacaggtgttcaa)(SEQ ID NO.88)对A118X位点腺嘌呤突变效率以及对应的TadA-TadA*片段在nCas9上插入位置进行Sanger测序分析(图2)。在筛选的文库中回收的43个插入位点中，有9个位点聚集在短片段(16-aa)内，它们位于1048Thr，1050Ile，1051Thr，1052Leu，1054Asn，1056Glu，1057Ile，1059Lys和1063Ile的位置。这些位点在筛选库中的富集是特定的，因为在未筛选的库中，这些位点分别仅被插入了61、39、90、38、5、29、76、53和25次，比某些位置少得多筛选后未恢复的其他位点(例如1090Pro，插入280次)。因此，16个氨基酸的片段对外源片段插入具有高度的耐受性，并且可能对于nCas9功能是非必需的。该片段在28个SpCas9直系同源物中不保守(图3)。因此，在后续构建真核表达载体时，我们用TadA-TadA*替换了1048Thr-1063Ile区，以生成CE-ABE^1048-1063。

7、比较ABEmax和多种CE-ABE在人细胞中靶向编辑效率

在原核细胞中筛选得到功能性的CE-ABE后，我们进一步检测了CE-ABE在HEK293T细胞中检测靶向碱基编辑效率，过程如下：

首先分别构建CE-ABE真核表达载体：

利用正向引物(agggagagccgccaccatgaaacggacagccgac)(SEQ ID NO.89)和反向引物(tcctcttcttcttgggctcgaattcgctgccgtcggc)(SEQ ID NO.90)对上述43个TadA-TadA*片段成功插入并具有腺嘌呤脱氨作用的编辑器进行PCR扩增，得到20个CE-ABE片段。

利用正向引物(ggtggcggctctccctatagtgagtc)(SEQ ID NO.91)和反向引物(cccaagaagaagaggaaagtctaacc)(SEQ ID NO.92)对pCMV-ABEmax进行扩增，得到片段SEQID NO.35。

用诺唯赞高保真酶试剂盒(Vazyme,p501-d2)PCR扩增片段。PCR反应体系如下所示：

表15

水	补加至50μL
		2xbuffer	25μL
dNTP	1μL
		正向引物(10μM)	2μL
反向引物(10μM)	2μL
		高保真酶	1μL
细胞裂解液	3-5μL

PCR程序如下：

表16

PCR扩增产物经AxyPrep PCR Clean-up试剂盒(Axygen,AP-PCR-500G)纯化回收，并且进行重组反应，再使用Gibson Assembly Master Mix重组试剂盒(NEB，E2611S)对该片段进行重组，反应体系如下：

表17

Gibson Assembly Master Mix(2X)	5μl
		CE-ABE的PCR片段	150ng
pCMV-ABE的PCR片段(SEQ ID NO.35)	50ng
		无菌水	补水至10μl

反应液混合后置于50℃孵育1h，随后进行转化，复苏30min，涂板于氨卞抗性的LB琼脂平板，37℃培养过夜。挑选单克隆进行测序验证，得到pCMV-CE-ABE质粒(SEQ IDNO.36-55)。采用AxyPrep plasmids miniprep试剂盒(Axygen,AP-MN-P-250G)进行质粒小提。进行Sanger测序。

复苏HEK293FT细胞(来自ATCC)，并培养在10cm培养皿(Corning,430167)中，培养基为含有10％体积比的胎牛血清(HyClone,SV30087)的DMEM(HyClone,SH30243.01)。培养温度为37℃，二氧化碳浓度为5％。传代后当细胞密度为80％时，细胞分盘至12孔板。12孔板使用前用1:10稀释的多聚赖氨酸溶液(Sigma，P4707-50ML)包被处理。

1)接种细胞12-14h后，细胞浓度约为80％时，进行转染。每孔转染的质粒的量是CE-ABE(SEQ ID NO.36-55)质粒700ng，ABEmax-site1(SEQ ID NO.12)的sgRNA300ng将质粒混在100μL的Opti-MEM(Gibco,11058021)培养基中。以pCMV-ABEmax作为阳性对照组，每孔加700ng质粒(Addgene，#112095)和ABEmax-site1(SEQ ID NO.12)的sgRNA 300ng。

2)另外，将3μl的Lipofectamine 2000转染试剂(Thermo,11668019)混入100μl的Opti-MEM培养基，静置5分钟。

3)将混有质粒的Opti-MEM加入混有Lipofectamine 2000的Opti-MEM，慢速吹打混匀，静置20分钟。

4)将上述混和静置好的转染液分别加入培养的细胞中。

5)转染6小时后用含有10％FBS的DMEM换液。

6)转染48小时后，去培基，用PBS清洗一次细胞，然后用TE(Thermo Fisher，R001100)将细胞消化下来，再用含有10％FBS的DMEM终止消化，并离心收集细胞，最后用培养基重悬。

7)重悬后的细胞进行FACS(Fluorescence activated Cell Sorting)分选，收集GFP荧光强度前5％的细胞，每个样品至少收集5000个细胞。

取上述收集的细胞的1/6进行直接裂解，并PCR扩增靶向位点片段，正向引物：aaagatcttcacaggctaccccc(SEQ ID NO.103)；反向引物：aatccacagcaacaccctctcc(SEQ IDNO.104)。用诺唯赞高保真酶试剂盒(Vazyme,p501-d2)PCR扩增各基因组靶向位点片段。PCR反应体系如下所示：

表18

PCR程序如下：

表19

PCR扩增产物经AxyPrep PCR Clean-up试剂盒(Axygen,AP-PCR-500G)纯化回收，进行Sanger测序。测序情况与CE-ABE对应的插入位点对应结果如图4所示。

8、比较ABEmax和CE-ABE在人细胞中的脱靶情况

收集上述5％GFP阳性细胞30000个，离心后去上清，然后加入TRIzol(ThermoFisher，15596018)试剂，按照说明书进行总RNA的抽提。然后取部分进行反转录，详细步骤如下：

1)抽取总RNA：加入1ml TRIzol试剂，吹打数次，使细胞均质化，吸取TRIzol于无核酸酶的离心管中。然后加入200μL三氯甲烷，混合均匀，4℃预冷离心机中12000rpm离心15分钟；小心吸取400μL上清于新的无核酸酶离心管中，加入400μL异丙醇，室温混匀后静置10分钟；4℃预冷离心机中12000rpm离心15分钟，弃上清；加入75％乙醇1ml，混匀后于4℃预冷离心机中12000rpm离心15分钟，弃上清，自然晾干，加入无核酸酶的水20-30μL，使用NanoDrop进行RNA浓度测定。

2)总RNA反转录成cDNA，我们使用

II Q RT SuperMix for qPCR(+g DNAwiper)试剂盒进行，首先去除总RNA中的基因组DNA 500ng总RNA 2μL 4×gDNA wiper Mix(Vazyme，R223-01)补水至8μL，42℃孵育5分钟。然后开始反转录反应，向上述8μL反应液中加入

II qRT SuperMix II^a(Vazyme，R223-01)，50℃孵育20分钟，再置于85℃反应2分钟灭活反转录酶活性。即可得到cDNA，进行后续检测。

在之前的ABEmax转染细胞的RNA-seq数据中分析得到三个脱靶编辑高的RNA脱靶位点(chr19(14518195)，chr11(62594034)，chr16(25164711))，针对这三个位点进行引物设计，对CE-ABE的cDNA样本进行这三个位点的扩增，随后进行Sanger测序分析，结果如图5所示。通过分析可以发现，相较于ABEmax，所有CE-ABE在检测的3个RNA脱靶位点上都有显著性的降低。这说明嵌合脱氨酶在nCas9的内部，能够有效的降低TadA-TadA*在部分RNA位点上的脱靶编辑(图6)。

随后，我们针对CE-ABE^1048-1063和CE-ABE¹⁰⁷²(编号后的数字为TadA-TadA*片段在nCas9内部的插入位点)以及ABEmax转染的细胞RNA进行全转录组测序，所有RNA样品均使用位于中国北京的Novogene生物信息学研究所的Illumina HiSeq X 10(2×150PE)进行测序，每个样品的读取深度约为2000万。通过STAR软件(版本2.5.1)将读段映射到人参考基因组(hg38)，使用来自GENCODE v30版的注释。删除重复后，通过GATK HaplotypeCaller(版本4.1.2)识别变体，并用QD(质量按深度)过滤，所有变体均通过bam-readcount进行验证并量化，参数为-q 20-b 30。给定的编辑至少应为10倍，并且要求这些编辑至少要有99％的读数支持野生型样品中的参考等位基因。最后，仅考虑转录链中的A到G(对于ABE)编辑用于下游分析。具体结果如图6，嵌合在1048Thr-1063Ile和1072Val位置的CE-ABE能够在全转录组水平极大的降低TadA-TadA*在RNA上的脱靶编辑。

同时还对ABEmax、CE-ABE^1048-1063和CE-ABE¹⁰⁷²三个编辑器靶向编辑效率进行监测。结果显示，虽然CE-ABE-1072的靶向效率显著低于ABEmax，而CE-ABE^1048-1063的靶向编辑效率与ABEmax没有显著差异，具体结果如图7所示。

9、CE-ABE^1048-1063在多个内源基因位点上的碱基编辑结果

我们进一步在HEK293T和N2a细胞中表征了CE-ABE^1048-1063的靶向碱基编辑效率以及编辑窗口，过程如下：

复苏HEK293FT和N2a细胞(来自ATCC)，并分别培养在10cm培养皿(Corning,430167)中，培养基为含有10％体积比的胎牛血清(HyClone,SV30087)的DMEM(HyClone,SH30243.01)。培养温度为37℃，二氧化碳浓度为5％。传代后当细胞密度为80％时，细胞分盘至12孔板。12孔板使用前用1:10稀释的多聚赖氨酸溶液(Sigma，P4707-50ML)包被处理。

2)接种细胞12-14h后，细胞浓度约为80％时，进行转染。每孔转染的质粒的量是CE-ABE^1048-1063(SEQ ID NO.45)质粒700ng，对于HEK293FT细胞，各个位点(SEQ ID NO.13-20)的gRNA质粒300ng；对于N2a细胞，各个位点(SEQ ID NO.21-32)的gRNA质粒300ng。将质粒混在100μL的Opti-MEM(Gibco,11058021)培养基中。以pCMV-AncBE4max作为对照，每个孔加700ng pCMV-ABE4max质粒和300ng各位点的sgRNA。

3)另外，将3μl的Lipofectamine 2000转染试剂(Thermo,11668019)混入100μl的Opti-MEM培养基，静置5分钟。

4)将混有质粒的Opti-MEM加入混有Lipofectamine 2000的Opti-MEM，慢速吹打混匀，静置20分钟。

5)将上述混和静置好的转染液分别加入培养的细胞中。

6)转染6小时后用含有10％FBS的DMEM换液。转染48小时后，去培基，用PBS清洗一次细胞，然后用TE(Thermo Fisher，R001100)将细胞消化下来，再用含有10％FBS的DMEM终止消化，并离心收集细胞，最后用培养基重悬。

7)重悬后的细胞进行FACS(Fluorescence activated Cell Sorting)分选，由于GFP信号在gRNA质粒上，因此，我们直接分选所有的GFP阳性细胞，每个样品至少收集5000个细胞。

上述收集的细胞直接进行裂解，并PCR扩增靶向位点片段。用诺唯赞高保真酶试剂盒(Vazyme,p501-d2)PCR扩增各基因组靶向位点片段。PCR反应体系如下所示：

表20

PCR程序如下：

表21

PCR扩增产物经AxyPrep PCR Clean-up试剂盒(Axygen,AP-PCR-500G)纯化回收。将具有不同条形码的PCR产物集中在一起，在中国北京的Novogene生物信息研究所的Illumina Hiseq X Ten(2×150PE)平台上进行深度测序。使用AdapterRemoval版本2.2.2删除了配对末端读取的适配器对，并将11bp或更多碱基的配对末端读取比对合并为单个共有读取。然后使用BWA-MEM算法(BWA v0.7.16)将所有处理的读段映射到靶序列。对于每个位点，使用参数-q 20-b 30的bam读数计数来计算突变率。基于原型间隔子中至少包含1个插入或缺失的核苷酸的读数计算插入缺失。插入缺失频率计算为含插入缺失的读段数/总映射读段数。测序情况结果见附图8和9。结果表明CE-ABE^1048-1063在HEK293T细胞的多个内源位点的靶向碱基编辑效率与ABE4max相当。另外，CE-ABE^1048-1063编辑窗口没有发生显著改变，具体结果见附图8和9。

9、CE-CBE^1048-1063在多个内源基因位点上的碱基编辑结果

在上述实验中已经发现将nCas9上1048Thr-106Ile之间的片段替换成TadA-TadA*的CE-ABE靶向效率最高，并且脱靶效率较低。进一步将APOBEC1(SEQ ID NO.68)和APOBEC3A(SEQ ID NO.69)分别替换nCas9的1048Thr-1063Ile肽段，并在HEK293T细胞中表征了CE-CBE^1048-1063的靶向碱基编辑效率以及编辑窗口，过程如下：

1)首先分别构建CE-CBE^1048-1063真和CE-A3A^1048-1063核表达载体：

利用正向引物：catgaactttttcaagtccggaTCCgagaccccaggc(SEQ ID NO.93)和反向引物：tttcgccgtttgtctcgctctctggtgttgctgac(SEQ ID NO.94)对APOBEC1片段进行PCR扩增。

利用正向引物：catgaactttttcaagtccggaTCCgagaccccaggc(SEQ ID NO.95)和反向引物：tttcgccgtttgtctcgctctctggtgttgctgac(SEQ ID NO.96)对APOBEC3A片段进行PCR扩增。

利用正向引物：gagacaaacggcgaaaccggggagatc(SEQ ID NO.97)和反向引物：cttgaaaaagttcatgatgttgc(SEQ ID NO.98)以pCMV-AncBE4max为模板PCR扩增。

表22

水	补加至50μL
		2xbuffer	25μL
dNTP	1μL
		正向引物(10μM)	2μL
反向引物(10μM)	2μL
		高保真酶	1μL
模板DNA	1μL

PCR程序如下：

表23

表24

Gibson Assembly Master Mix(2X)	5μl
		APOBEC1和APOBEC3A的PCR片段	150ng
pCMV-AncBE4max的PCR片段	50ng
		无菌水	补水至10μl

反应液混合后置于50℃孵育1h，随后进行转化，复苏30min，涂板于氨卞抗性的LB琼脂平板，37℃培养过夜。挑选单克隆进行测序验证，得到pCMV-CE-CBE^1048-1063质粒(SEQ IDNO.56)和pCMV-CE-A3A^1048-1063质粒(SEQ ID NO.70)。采用AxyPrep plasmids miniprep试剂盒(Axygen,AP-MN-P-250G)进行质粒小提。进行Sanger测序。

复苏HEK293FT细胞(来自ATCC)，并分别培养在10cm培养皿(Corning,430167)中，培养基为含有10％体积比的胎牛血清(HyClone,SV30087)的DMEM(HyClone,SH30243.01)。培养温度为37℃，二氧化碳浓度为5％。传代后当细胞密度为80％时，细胞分盘至12孔板。12孔板使用前用1:10稀释的多聚赖氨酸溶液(Sigma，P4707-50ML)包被处理。

2)接种细胞12-14h后，细胞浓度约为80％时，进行转染。每孔转染的质粒的量是CE-CBE(SEQ ID NO.56)质粒或CE-A3A(SEQ ID NO.70)700ng，各个位点的gRNA质粒300ng(SEQ ID NO.57-67)。将质粒混在100μL的Opti-MEM(Gibco,11058021)培养基中。以pCMV-AncBE4max作为对照，每个孔加700ng pCMV-AncBE4max质粒和300ng各位点的sgRNA。

5)将上述混和静置好的转染液分别加入培养的细胞中。

表25

PCR程序如下：

表26

PCR扩增产物经AxyPrep PCR Clean-up试剂盒(Axygen,AP-PCR-500G)纯化回收。将具有不同条形码的PCR产物集中在一起，在中国北京的Novogene生物信息研究所的Illumina Hiseq X Ten(2×150PE)平台上进行深度测序。使用AdapterRemoval版本2.2.2删除了配对末端读取的适配器对，并将11bp或更多碱基的配对末端读取比对合并为单个共有读取。然后使用BWA-MEM算法(BWA v0.7.16)将所有处理的读段映射到靶序列。对于每个位点，使用参数-q 20-b 30的bam读数计数来计算突变率。基于原型间隔子中至少包含1个插入或缺失的核苷酸的读数计算插入缺失。插入缺失频率计算为含插入缺失的读段数/总映射读段数。测序情况结果见附图10和11。结果表明CE-CBE在HEK293T细胞的多个内源位点的靶向碱基编辑效率与BE4max相当。另外，CE-CBE编辑窗口没有发生显著改变，具体结果见附图8和附图10和11。

11、CE-CBE和CE-A3A在人细胞内RNA脱靶编辑结果

利用FACS分选收集上述5％GFP阳性细胞30000个，离心后去上清，然后加入TRIzol(Thermo Fisher，15596018)试剂，按照说明书进行总RNA的抽提。然后取部分进行反转录，详细步骤如下：

抽取总RNA：加入1ml TRIzol试剂，吹打数次，使细胞均质化，吸取TRIzol于无核酸酶的离心管中。然后加入200μL三氯甲烷，混合均匀，4℃预冷离心机中12000rpm离心15分钟；小心吸取400μL上清于新的无核酸酶离心管中，加入400μL异丙醇，室温混匀后静置10分钟；4℃预冷离心机中12000rpm离心15分钟，弃上清；加入75％乙醇1ml，混匀后于4℃预冷离心机中12000rpm离心15分钟，弃上清，自然晾干，加入无核酸酶的水20-30μL，使用NanoDrop进行RNA浓度测定。

随后，针对BE4max、CE-CBE^1048-1063、CE-CBE¹⁰⁷²、BE-A3A、CE-A3A^1048-1063、CE-A3A¹⁰⁷²进行全转录组测序，所有RNA样品均使用位于中国北京的Novogene生物信息学研究所的Illumina HiSeq X 10(2×150PE)进行测序，每个样品的读取深度约为2000万。通过STAR软件(版本2.5.1)将读段映射到人参考基因组(hg38)，使用来自GENCODE v30版的注释。删除重复后，通过GATK HaplotypeCaller(版本4.1.2)识别变体，并用QD(质量按深度)过滤，所有变体均通过bam-readcount进行验证并量化，参数为-q 20-b 30。给定的编辑至少应为10倍，并且要求这些编辑至少要有99％的读数支持野生型样品中的参考等位基因。最后，仅考虑转录链中的C到T编辑用于下游分析。附图12和13表明嵌合在1048Thr-1063Ile和1072Val位置的CE-CBE^1048-1063、CE-CBE¹⁰⁷²、CE-CBE^1048-1063和CE-CBE¹⁰⁷²都能够在全转录组水平极大的降低APOBEC1和APOBEC3A在RNA上的脱靶编辑。

12、CE-CBE^1048-1063和CE-A3A^1048-1063在小鼠胚胎中产生的脱靶DNA编辑结果

对CE-CBE^1048-1063和CE-A3A^1048-1063进行体外转录成mRNA，首先采用正向引物：ATGCCTGCTATTGTCTTCCCAA(SEQ ID NO.99)，和反向引物：AACGGGACTTTCCAAAATGTC(SEQ IDNO.100)，分别对CE-CBE^1048-1063和CE-A3A^1048-1063进行PCR扩增，得到线性化片段CE-CBE¹⁰⁴⁸ ^-1063和CE-A3A^1048-1063。对于sgRNA的转录，首先合成寡聚核苷酸链，退火后，连接到线性化的PUC57-Sp sgRNA质粒中，构建的PUC57质粒通过Sanger测序确认后使用正向引物：TCTCGCGCGTTTCGGTGATGACGG(SEQ ID NO.101)和反向引物：AAAAAAATCTCGCCAACAAGTTGAC(SEQ ID NO.102)对sgRNA进行PCR扩增：

具体步骤如下：

表27

PCR程序如下：

表28

无核酸酶环境下进行以下操作：首先，按照1:25的比例向PCR产物中加RNAsecure^TMRNase Inactivation Reagent(Invitrogen^TM，AM7005)，60℃干浴处理10分钟；然后使用MinElute PCR Purification Kit(QIAGEN，28004)试剂盒回收PCR片段。

(1)Cas9的体外转录

按照试剂盒mMESSAGE mMACHINE^TM T7 ULTRA Transcription Kit(Invitrogen^TM，AM1345)说明书进行Cas9的体外转录，如下加反应液：

10μL T7 2×NTP/ARCA

2μL 10×T7Reaction Buffer

600ng模板Cas9PCR片段

2μL T7 Enzyme Mix

加Nuclease-free Water至20μL

反应液混合均匀后在PCR热循环仪上进行反应，热盖温度设为50℃，体系温度设为37℃；反应进行2小时后加1μL TURBO DNase消化模板DNA，37℃反应15分钟。然后进行后续加Poly-A反应，体系如下：

20μL上述转录产物

20μL 5×E-PAP Buffer

10μL 25mM MnCl₂

10μL ATP Solution

36μL Nuclease-free Water

在加入E-PAP酶之前，吸取2.5μL混合反应液用做后续凝胶电泳，然后向96μL反应液中加入4μL E-PAP酶，37℃反应30分钟，吸取2.5μL加尾后的反应液，与加尾前的反应液一起在0.8％琼脂糖凝胶中进行电泳，电压为180V，时间为10分钟。确认条带正常后，使用RNeasy Mini Kit(QIAGEN，74104)试剂盒回收Cas9mRNA。

(2)sgRNA的体外转录

上述得到的纯化产物进行后续步骤。按照试剂盒MEGAshortscript^TMT7Transcription Kit(Invitrogen^TM，AM1354)说明书进行sgRNA的体外转录，600ng模板DNA用于反应，如下混合反应液：

1μL T7 10×Reaction Buffer

1μL T7 ATP Solution(75mM)

1μL T7 CTP Solution(75mM)

1μL T7 GTP Solution(75mM)

1μL T7 UTP Solution(75mM)

1μL T7 Enzyme Mix

2μL T7 Enzyme Mix

600ng模板sgRNA PCR片段

加Nuclease-free Water至20μL

反应液混合均匀后在PCR热循环仪上进行反应，热盖温度设为50℃，体系温度设为37℃。反应进行6小时后，加1μL TURBO DNase对模板DNA进行消化，37℃反应15分钟，然后吸取1μL混合反应液在0.8％琼脂糖凝胶中进行电泳，电压为180V，时间为10分钟。确定目的条带正常后，用MEGAclear Kit(Invitrogen^TM，AM1908)试剂盒回收sgRNA的mRNA。

(3)受精卵注射和胚胎移植

取6-8周龄C57雌鼠，腹腔注射人绒毛膜促性腺激素HCG(宁波三生药物，B141002)，48小时后腹腔注射孕马血清促性腺激素PMSG(宁波三生药物，S141004)，然后与7-8周龄C57雄鼠进行合笼。12小时后麻醉处死小鼠，取卵细胞，待受精卵发育到2细胞时期，将细胞分开，其中一个细胞转移到另一个透明带中，不注射，直接与其余20-25枚ICR小鼠受精卵一起转移到假孕ICR雌性小鼠的输卵管中。

分别将CBE4max/CE-CBE^1048-1063/CE-A3A^1048-1063(100ng/μL)与sgRNA(50ng/μL)的mRNA混合，然后12000rpm离心5分钟，吸取上清mRNA并使用FemtoJet显微注射器在含有5μg/ml细胞松弛素B的HEPES-CZB培养基液滴中注入剩下的细胞胞质中。然后，将注射的受精卵培养至双细胞阶段，并与其余20-25枚ICR小鼠受精卵一起转移到假孕ICR雌性小鼠的输卵管中。

13.5天后，解剖母鼠，观察小鼠眼睛颜色，选出C57小鼠胚胎，进行裂解，并提取基因组DNA，进行后续检测。首先检测sgRNA靶向效率，确定编辑效率，具体结果如图14所示。随后，对基因组DNA分别进行WGS测序分析编辑器在DNA上的脱靶情况，具体结果如图15和图16所示。可见，CE-CBE^1048-1063和CE-A3A^1048-1063在小鼠胚胎中具有更好的编辑效率和更低的脱靶率。

综上所述，本发明有效克服了现有技术中的种种缺点而具高度产业利用价值。

上述实施例仅例示性说明本发明的原理及其功效，而非用于限制本发明。任何熟悉此技术的人士皆可在不违背本发明的精神及范畴下，对上述实施例进行修饰或改变。因此，举凡所属技术领域中具有通常知识者在未脱离本发明所揭示的精神与技术思想下所完成的一切等效修饰或改变，仍应由本发明的权利要求所涵盖。

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Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser

1 5 10 15

Gly Ser Glu Thr Gly Pro Val Ala Val Asp Pro Thr Leu Arg Arg Arg

20 25 30

Ile Glu Pro His Glu Phe Glu Val Phe Phe Asp Pro Arg Glu Leu Arg

35 40 45

Lys Glu Thr Cys Leu Leu Tyr Glu Ile Lys Trp Gly Thr Ser His Lys

50 55 60

Ile Trp Arg His Ser Ser Lys Asn Thr Thr Lys His Val Glu Val Asn

65 70 75 80

Phe Ile Glu Lys Phe Thr Ser Glu Arg His Phe Cys Pro Ser Thr Ser

85 90 95

Cys Ser Ile Thr Trp Phe Leu Ser Trp Ser Pro Cys Gly Glu Cys Ser

100 105 110

Lys Ala Ile Thr Glu Phe Leu Ser Gln His Pro Asn Val Thr Leu Val

115 120 125

Ile Tyr Val Ala Arg Leu Tyr His His Met Asp Gln Gln Asn Arg Gln

130 135 140

Gly Leu Arg Asp Leu Val Asn Ser Gly Val Thr Ile Gln Ile Met Thr

145 150 155 160

Ala Pro Glu Tyr Asp Tyr Cys Trp Arg Asn Phe Val Asn Tyr Pro Pro

165 170 175

Gly Lys Glu Ala His Trp Pro Arg Tyr Pro Pro Leu Trp Met Lys Leu

180 185 190

Tyr Ala Leu Glu Leu His Ala Gly Ile Leu Gly Leu Pro Pro Cys Leu

195 200 205

Asn Ile Leu Arg Arg Lys Gln Pro Gln Leu Thr Phe Phe Thr Ile Ala

210 215 220

Leu Gln Ser Cys His Tyr Gln Arg Leu Pro Pro His Ile Leu Trp Ala

225 230 235 240

Thr Gly Leu Lys Ser Gly Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu

245 250 255

Ser Ala Thr Pro Glu Ser

260

<210> 4

<211> 239

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 4

Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser

1 5 10 15

Gly Ser Met Glu Ala Ser Pro Ala Ser Gly Pro Arg His Leu Met Asp

20 25 30

Pro His Ile Phe Thr Ser Asn Phe Asn Asn Gly Ile Gly Arg His Lys

35 40 45

Thr Tyr Leu Cys Tyr Glu Val Glu Arg Leu Asp Asn Gly Thr Ser Val

50 55 60

Lys Met Asp Gln His Arg Gly Phe Leu His Asn Gln Ala Lys Asn Leu

65 70 75 80

Leu Cys Gly Phe Tyr Gly Arg His Ala Glu Leu Arg Phe Leu Asp Leu

85 90 95

Val Pro Ser Leu Gln Leu Asp Pro Ala Gln Ile Tyr Arg Val Thr Trp

100 105 110

Phe Ile Ser Trp Ser Pro Cys Phe Ser Trp Gly Cys Ala Gly Glu Val

115 120 125

Arg Ala Phe Leu Gln Glu Asn Thr His Val Arg Leu Arg Ile Phe Ala

130 135 140

Ala Arg Ile Tyr Tyr Tyr Asp Pro Leu Tyr Lys Glu Ala Leu Gln Met

145 150 155 160

Leu Arg Asp Ala Gly Ala Gln Val Ser Ile Met Thr Tyr Asp Glu Phe

165 170 175

Lys His Cys Trp Asp Thr Phe Val Asp His Gln Gly Cys Pro Phe Gln

180 185 190

Pro Trp Asp Gly Leu Asp Glu His Ser Gln Ala Leu Ser Gly Arg Leu

195 200 205

Arg Ala Ile Leu Gln Asn Gln Gly Asn Ser Gly Ser Glu Ser Gly Ser

210 215 220

Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser

225 230 235

<210> 5

<211> 83

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 5

Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu Val

1 5 10 15

Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val Ile

20 25 30

Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp Glu

35 40 45

Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala Pro Glu Tyr

50 55 60

Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu Asn Lys Ile

65 70 75 80

Lys Met Leu

<210> 6

<211> 18

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 6

Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys Arg

1 5 10 15

Lys Val

<210> 7

<211> 16

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 7

Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser

1 5 10 15

<210> 8

<211> 10

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 8

Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser

1 5 10

<210> 9

<211> 1840

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 9

Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys Arg

1 5 10 15

Lys Val Ser Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr

20 25 30

Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser

35 40 45

Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys

50 55 60

Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala

65 70 75 80

Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn

85 90 95

Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val

100 105 110

Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu

115 120 125

Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu

130 135 140

Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys

145 150 155 160

Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala

165 170 175

Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp

180 185 190

Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val

195 200 205

Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly

210 215 220

Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg

225 230 235 240

Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu

245 250 255

Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys

260 265 270

Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp

275 280 285

Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln

290 295 300

Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu

305 310 315 320

Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu

325 330 335

Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr

340 345 350

Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu

355 360 365

Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly

370 375 380

Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu

385 390 395 400

Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp

405 410 415

Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln

420 425 430

Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe

435 440 445

Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr

450 455 460

Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg

465 470 475 480

Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn

485 490 495

Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu

500 505 510

Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro

515 520 525

Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr

530 535 540

Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser

545 550 555 560

Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg

565 570 575

Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu

580 585 590

Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala

595 600 605

Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp

610 615 620

Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu

625 630 635 640

Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys

645 650 655

Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg

660 665 670

Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly

675 680 685

Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser

690 695 700

Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser

705 710 715 720

Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly

725 730 735

Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile

740 745 750

Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys

755 760 765

Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg

770 775 780

Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met

785 790 795 800

Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys

805 810 815

Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu

820 825 830

Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp

835 840 845

Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser

850 855 860

Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp

865 870 875 880

Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys

885 890 895

Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr

900 905 910

Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser

915 920 925

Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg

930 935 940

Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr

945 950 955 960

Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr

965 970 975

Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr

980 985 990

Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu

995 1000 1005

Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu

1010 1015 1020

Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met

1025 1030 1035 1040

Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe

1045 1050 1055

Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Ser Gly Ser Glu Thr Pro

1060 1065 1070

Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Ser Glu Thr Gly Pro

1075 1080 1085

Val Ala Val Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His Glu Phe

1090 1095 1100

Glu Val Phe Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys Leu Leu

1105 1110 1115 1120

Tyr Glu Ile Lys Trp Gly Thr Ser His Lys Ile Trp Arg His Ser Ser

1125 1130 1135

Lys Asn Thr Thr Lys His Val Glu Val Asn Phe Ile Glu Lys Phe Thr

1140 1145 1150

Ser Glu Arg His Phe Cys Pro Ser Thr Ser Cys Ser Ile Thr Trp Phe

1155 1160 1165

Leu Ser Trp Ser Pro Cys Gly Glu Cys Ser Lys Ala Ile Thr Glu Phe

1170 1175 1180

Leu Ser Gln His Pro Asn Val Thr Leu Val Ile Tyr Val Ala Arg Leu

1185 1190 1195 1200

Tyr His His Met Asp Gln Gln Asn Arg Gln Gly Leu Arg Asp Leu Val

1205 1210 1215

Asn Ser Gly Val Thr Ile Gln Ile Met Thr Ala Pro Glu Tyr Asp Tyr

1220 1225 1230

Cys Trp Arg Asn Phe Val Asn Tyr Pro Pro Gly Lys Glu Ala His Trp

1235 1240 1245

Pro Arg Tyr Pro Pro Leu Trp Met Lys Leu Tyr Ala Leu Glu Leu His

1250 1255 1260

Ala Gly Ile Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg Arg Lys

1265 1270 1275 1280

Gln Pro Gln Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys His Tyr

1285 1290 1295

Gln Arg Leu Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys Ser Gly

1300 1305 1310

Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser

1315 1320 1325

Glu Thr Asn Gly Glu Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp

1330 1335 1340

Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile Val

1345 1350 1355 1360

Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu

1365 1370 1375

Pro Lys Arg Asn Ser Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp

1380 1385 1390

Pro Lys Lys Tyr Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val

1395 1400 1405

Leu Val Val Ala Lys Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser

1410 1415 1420

Val Lys Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu

1425 1430 1435 1440

Lys Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys

1445 1450 1455

Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu

1460 1465 1470

Asn Gly Arg Lys Arg Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly

1475 1480 1485

Asn Glu Leu Ala Leu Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala

1490 1495 1500

Ser His Tyr Glu Lys Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys

1505 1510 1515 1520

Gln Leu Phe Val Glu Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu

1525 1530 1535

Gln Ile Ser Glu Phe Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu

1540 1545 1550

Asp Lys Val Leu Ser Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg

1555 1560 1565

Glu Gln Ala Glu Asn Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly

1570 1575 1580

Ala Pro Ala Ala Phe Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Arg

1585 1590 1595 1600

Tyr Thr Ser Thr Lys Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser

1605 1610 1615

Ile Thr Gly Leu Tyr Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly

1620 1625 1630

Asp Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Thr Asn Leu Ser Asp

1635 1640 1645

Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu Val Ile Gln Glu Ser Ile

1650 1655 1660

Leu Met Leu Pro Glu Glu Val Glu Glu Val Ile Gly Asn Lys Pro Glu

1665 1670 1675 1680

Ser Asp Ile Leu Val His Thr Ala Tyr Asp Glu Ser Thr Asp Glu Asn

1685 1690 1695

Val Met Leu Leu Thr Ser Asp Ala Pro Glu Tyr Lys Pro Trp Ala Leu

1700 1705 1710

Val Ile Gln Asp Ser Asn Gly Glu Asn Lys Ile Lys Met Leu Ser Gly

1715 1720 1725

Gly Ser Gly Gly Ser Gly Gly Ser Thr Asn Leu Ser Asp Ile Ile Glu

1730 1735 1740

Lys Glu Thr Gly Lys Gln Leu Val Ile Gln Glu Ser Ile Leu Met Leu

1745 1750 1755 1760

Pro Glu Glu Val Glu Glu Val Ile Gly Asn Lys Pro Glu Ser Asp Ile

1765 1770 1775

Leu Val His Thr Ala Tyr Asp Glu Ser Thr Asp Glu Asn Val Met Leu

1780 1785 1790

Leu Thr Ser Asp Ala Pro Glu Tyr Lys Pro Trp Ala Leu Val Ile Gln

1795 1800 1805

Asp Ser Asn Gly Glu Asn Lys Ile Lys Met Leu Ser Gly Gly Ser Lys

1810 1815 1820

Arg Thr Ala Asp Gly Ser Glu Phe Glu Pro Lys Lys Lys Arg Lys Val

1825 1830 1835 1840

<210> 10

<211> 1817

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 10

Lys Arg Thr Ala Asp Gly Ser Glu Phe Glu Ser Pro Lys Lys Lys Arg

1 5 10 15

Lys Val Ser Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr

20 25 30

Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser

35 40 45

Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys

50 55 60

Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala

65 70 75 80

Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn

85 90 95

Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val

100 105 110

Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu

115 120 125

Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu

130 135 140

Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys

145 150 155 160

Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala

165 170 175

Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp

180 185 190

Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val

195 200 205

Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly

210 215 220

Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg

225 230 235 240

Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu

245 250 255

Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys

260 265 270

Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp

275 280 285

Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln

290 295 300

Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu

305 310 315 320

Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu

325 330 335

Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr

340 345 350

Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu

355 360 365

Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly

370 375 380

Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu

385 390 395 400

Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp

405 410 415

Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln

420 425 430

Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe

435 440 445

Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr

450 455 460

Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg

465 470 475 480

Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn

485 490 495

Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu

500 505 510

Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro

515 520 525

Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr

530 535 540

Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser

545 550 555 560

Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg

565 570 575

Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu

580 585 590

Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala

595 600 605

Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp

610 615 620

Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu

625 630 635 640

Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys

645 650 655

Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg

660 665 670

Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly

675 680 685

Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser

690 695 700

Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser

705 710 715 720

Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly

725 730 735

Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile

740 745 750

Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys

755 760 765

Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg

770 775 780

Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met

785 790 795 800

Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys

805 810 815

Glu His Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu

820 825 830

Tyr Tyr Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp

835 840 845

Ile Asn Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser

850 855 860

Phe Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp

865 870 875 880

Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys

885 890 895

Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr

900 905 910

Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser

915 920 925

Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg

930 935 940

Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr

945 950 955 960

Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr

965 970 975

Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr

980 985 990

Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu

995 1000 1005

Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu

1010 1015 1020

Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met

1025 1030 1035 1040

Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe

1045 1050 1055

Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Ser Gly Ser Glu Thr Pro

1060 1065 1070

Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Gly Ser Met Glu Ala Ser

1075 1080 1085

Pro Ala Ser Gly Pro Arg His Leu Met Asp Pro His Ile Phe Thr Ser

1090 1095 1100

Asn Phe Asn Asn Gly Ile Gly Arg His Lys Thr Tyr Leu Cys Tyr Glu

1105 1110 1115 1120

Val Glu Arg Leu Asp Asn Gly Thr Ser Val Lys Met Asp Gln His Arg

1125 1130 1135

Gly Phe Leu His Asn Gln Ala Lys Asn Leu Leu Cys Gly Phe Tyr Gly

1140 1145 1150

Arg His Ala Glu Leu Arg Phe Leu Asp Leu Val Pro Ser Leu Gln Leu

1155 1160 1165

Asp Pro Ala Gln Ile Tyr Arg Val Thr Trp Phe Ile Ser Trp Ser Pro

1170 1175 1180

Cys Phe Ser Trp Gly Cys Ala Gly Glu Val Arg Ala Phe Leu Gln Glu

1185 1190 1195 1200

Asn Thr His Val Arg Leu Arg Ile Phe Ala Ala Arg Ile Tyr Tyr Tyr

1205 1210 1215

Asp Pro Leu Tyr Lys Glu Ala Leu Gln Met Leu Arg Asp Ala Gly Ala

1220 1225 1230

Gln Val Ser Ile Met Thr Tyr Asp Glu Phe Lys His Cys Trp Asp Thr

1235 1240 1245

Phe Val Asp His Gln Gly Cys Pro Phe Gln Pro Trp Asp Gly Leu Asp

1250 1255 1260

Glu His Ser Gln Ala Leu Ser Gly Arg Leu Arg Ala Ile Leu Gln Asn

1265 1270 1275 1280

Gln Gly Asn Ser Gly Ser Glu Ser Gly Ser Gly Ser Glu Thr Pro Gly

1285 1290 1295

Thr Ser Glu Ser Ala Thr Pro Glu Ser Glu Thr Asn Gly Glu Thr Gly

1300 1305 1310

Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val

1315 1320 1325

Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr

1330 1335 1340

Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys

1345 1350 1355 1360

Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe

1365 1370 1375

Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu

1380 1385 1390

Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile

1395 1400 1405

Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu

1410 1415 1420

Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu

1425 1430 1435 1440

Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu

1445 1450 1455

Ala Ser Ala Gly Glu Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser

1460 1465 1470

Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys

1475 1480 1485

Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His

1490 1495 1500

Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys

1505 1510 1515 1520

Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr

1525 1530 1535

Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile

1540 1545 1550

His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr

1555 1560 1565

Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val

1570 1575 1580

Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr

1585 1590 1595 1600

Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Ser Gly Gly Ser Gly Gly

1605 1610 1615

Ser Gly Gly Ser Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly

1620 1625 1630

Lys Gln Leu Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val

1635 1640 1645

Glu Glu Val Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr

1650 1655 1660

Ala Tyr Asp Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp

1665 1670 1675 1680

Ala Pro Glu Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly

1685 1690 1695

Glu Asn Lys Ile Lys Met Leu Ser Gly Gly Ser Gly Gly Ser Gly Gly

1700 1705 1710

Ser Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu

1715 1720 1725

Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val

1730 1735 1740

Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp

1745 1750 1755 1760

Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala Pro Glu

1765 1770 1775

Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu Asn Lys

1780 1785 1790

Ile Lys Met Leu Ser Gly Gly Ser Lys Arg Thr Ala Asp Gly Ser Glu

1795 1800 1805

Phe Glu Pro Lys Lys Lys Arg Lys Val

1810 1815

<210> 11

<211> 1374

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 11

cggcgcacga aaaacgcgaa agcgtttcac gataaatgcg aaaactctgg aggatctagc 60

ggcggatcct ctggaagcga gacaccaggc acaagcgagt ccgccacacc agagagctcc 120

ggcggctcct ccggaggatc ctctgaggtg gagttttccc acgagtactg gatgagacat 180

gccctgaccc tggccaagag ggcatgggat gaaagagaag tccccgtggg cgccgtgctg 240

gtgcacaaca atagagtgat cggagaggga tggaacaggc caatcggccg ccacgaccct 300

accgcacacg cagagatcat ggcactgagg cagggaggcc tggtcatgca gaattaccgc 360

ctgatcgatg ccaccctgta tgtgacactg gagccatgcg tgatgtgcgc aggagcaatg 420

atccacagca ggatcggaag agtggtgttc ggagcacggg acgccaagac cggcgcagca 480

ggctccctga tggatgtgct gcaccacccc ggcatgaacc accgggtgga gatcacagag 540

ggaatcctgg cagacgagtg cgccgccctg ctgagcgatt tctttagaat gcggagacag 600

gagatcaagg cccagaagaa ggcacagagc tccaccgact ctggaggatc tagcggcgga 660

tcctctggaa gcgagacacc aggcacaagc gagtccgcca caccagagag ctccggcggc 720

tcctccggag gatcctctga ggtggagttt tcccacgagt actggatgag acatgccctg 780

accctggcca agagggcacg cgatgagagg gaggtgcctg tgggagccgt gctggtgctg 840

aacaatagag tgatcggcga gggctggaac agagccatcg gcctgcacga cccaacagcc 900

catgccgaaa ttatggccct gagacagggc ggcctggtca tgcagaacta cagactgatt 960

gacgccaccc tgtacgtgac attcgagcct tgcgtgatgt gcgccggcgc catgatccac 1020

tctaggatcg gccgcgtggt gtttggcgtg aggaacgcaa aaaccggcgc cgcaggctcc 1080

ctgatggacg tgctgcacta ccccggcatg aatcaccgcg tcgaaattac cgagggaatc 1140

ctggcagatg aatgtgccgc cctgctgtgc tatttctttc ggatgcctag acaggtgttc 1200

aatgctcaga agaaggccca gagctccacc gactccggag gatctagcgg aggctcctct 1260

ggctctgaga cacctggcac aagcgagagc gcaacacctg aaagcagcgg gggcagcagc 1320

ggggggtcag ttttcgcatt tatcgtgaaa cgctttcgcg tttttcgtgc gccg 1374

<210> 12

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 12

gaacacaaag catagactgc ggg 23

<210> 13

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 13

tacagcttgt agtactcata ggg 23

<210> 14

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 14

catatctcct aacttcaggt tgg 23

<210> 15

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 15

ggagtagggg ctcagcaggg cgg 23

<210> 16

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 16

gtatgaagac aataactata agg 23

<210> 17

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 17

ggaacagtgt gtagaggtgg ggg 23

<210> 18

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 18

ctgtatgggt cccggggcgc tgg 23

<210> 19

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 19

tgtgcacacg ctgcagagca tgg 23

<210> 20

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 20

gcgggacagc ccggaagtcc agg 23

<210> 21

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 21

attgatgtaa tggatgcagt ggg 23

<210> 22

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 22

gtttcagaat cgaagggtga agg 23

<210> 23

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 23

agacatattc ctcactacaa agg 23

<210> 24

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 24

ctttagcttg acatgcagcg cgg 23

<210> 25

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 25

agccaggtgg gcggttctct tgg 23

<210> 26

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 26

ccccacagga agtggccatg cgc 23

<210> 27

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 27

aattcactgt aaagctggaa agg 23

<210> 28

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 28

ctgtaaaaag gggctgctcc cgg 23

<210> 29

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 29

gccaaaacgt gaagaaataa tgg 23

<210> 30

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 30

agttaaaaga gaggggctcc cgg 23

<210> 31

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 31

ataaaaatgg atcccaacac tgg 23

<210> 32

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 32

acccaaggaa tcgaaaaccc agg 23

<210> 33

<211> 7629

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 33

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 3660

acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg 3720

aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc 3780

ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag 3840

gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg 3900

gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg 3960

gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc 4020

aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc 4080

gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac 4140

gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag 4200

ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac 4260

tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac 4320

gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag 4380

caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc 4440

aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac 4500

gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag 4560

ctgggaggtg actctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag 4620

aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat 4680

cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 4740

ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 4800

cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 4860

gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 4920

aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat 4980

catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 5040

gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa 5100

ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 5160

gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 5220

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 5280

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 5340

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 5400

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 5460

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 5520

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 5580

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 5640

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 5700

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 5760

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 5820

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 5880

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 5940

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 6000

ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 6060

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 6120

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 6180

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6240

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6300

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6360

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6420

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 6480

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6540

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6600

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6660

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6720

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6780

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6840

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6900

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6960

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 7020

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 7080

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7140

tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct 7200

ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt 7260

agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga 7320

atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt 7380

gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 7440

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 7500

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 7560

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 7620

aagtgtatc 7629

<210> 34

<211> 10864

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 34

gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc 60

accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac 120

agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc 180

acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat 240

ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg 300

gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac 360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa 420

ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg 480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg 540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc 600

aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg 660

ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg 720

attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat 780

gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag 840

atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg 900

ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg 960

atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag 1020

cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc 1080

tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa 1140

aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag 1200

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc 1260

attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag 1320

aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga 1380

ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg 1440

gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac 1500

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat 1560

aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc 1620

ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg 1680

aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc 1740

ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc 1800

aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg 1860

accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac 1920

ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg 1980

ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat 2040

ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc 2100

ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac 2160

gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg 2220

aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc 2280

gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg 2340

aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg 2400

gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat 2460

atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc 2520

gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac 2580

aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac 2640

tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc 2700

aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg 2760

gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact 2820

aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag 2880

ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac 2940

caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac 3000

cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg 3060

atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac 3120

atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct 3180

ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc 3240

accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag 3300

acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc 3360

agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat 3420

tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa 3480

gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt 3540

ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac 3600

tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag 3660

aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac 3720

tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag 3780

cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc 3840

ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc 3900

atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct 3960

gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag 4020

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac 4080

ctgtctcagc tgggaggtga ctctggcggc tcaaaaagaa ccgccgacgg cagcgaattc 4140

gagcccaaga agaagaggaa agtctaaccg gtcatcatca ccatcaccat tgagtttaaa 4200

cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 4260

ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 4320

aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 4380

acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 4440

tggcttctga ggcggaaaga accagctggg gctcgttgac agctagctca gtcctaggta 4500

taatactagt gctcttgccc ggcgtcaata cgttttagag ctagaaatag caagttaaaa 4560

taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgatccg 4620

gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta 4680

gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact 4740

atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 4800

tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 4860

tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 4920

tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 4980

gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 5040

agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 5100

cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 5160

agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag 5220

gtggcacttt tcggggaaat gtgggaaatg tgcgcggaac ccctatttgt ttatttttct 5280

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 5340

attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 5400

cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 5460

aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 5520

ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 5580

gtggcgcggt attatcccgt attgacgccg ggtaagagca actcggtcgc cgcatacact 5640

attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 5700

tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 5760

tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 5820

atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 5880

agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 5940

aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 6000

caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 6060

ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 6120

gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 6180

tcgctgagat aggtgcctca ctgattaagc attggtaagc gcggaacccc tatttgttta 6240

tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga aaaactcatc 6300

gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa 6360

aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc 6420

ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc 6480

gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa 6540

tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc 6600

atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg 6660

aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag 6720

gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg 6780

gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat 6840

aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc 6900

atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc 6960

gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca 7020

tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt 7080

ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt 7140

tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 7200

tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 7260

aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 7320

tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 7380

agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 7440

taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 7500

caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 7560

agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 7620

aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 7680

gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 7740

tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 7800

gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 7860

ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 7920

ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 7980

aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac 8040

accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta 8100

tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc 8160

gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc 8220

gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag 8280

ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc 8340

gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc 8400

atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg 8460

ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat 8520

gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg 8580

cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt 8640

gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc 8700

gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt 8760

gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat 8820

tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc 8880

acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa 8940

cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc 9000

gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc 9060

cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg 9120

gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag 9180

ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc 9240

tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 9300

cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga 9360

gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc 9420

cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata 9480

acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac caacgcgcag 9540

cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat 9600

cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc 9660

actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg 9720

ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat 9780

ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga 9840

gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt 9900

agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag 9960

cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct 10020

tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc 10080

cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa 10140

cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat 10200

cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg 10260

ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt 10320

cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt 10380

tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta 10440

ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat 10500

gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga 10560

aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga 10620

tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt 10680

agaggatcga gatcgatctc gatcccgcga aattaatacg actcactata ggggaattgt 10740

gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatataca 10800

tgccaccatg aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa 10860

agtc 10864

<210> 35

<211> 9243

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 35

gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc 60

accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac 120

agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc 180

acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat 240

ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg 300

gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac 360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa 420

ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg 480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg 540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc 600

aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg 660

ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg 720

attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat 780

gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag 840

atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg 900

ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg 960

atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag 1020

cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc 1080

tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa 1140

aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag 1200

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc 1260

attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag 1320

aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga 1380

ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg 1440

gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac 1500

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat 1560

aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc 1620

ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg 1680

aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc 1740

ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc 1800

aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg 1860

accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac 1920

ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg 1980

ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat 2040

ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc 2100

ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac 2160

gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg 2220

aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc 2280

gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg 2340

aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg 2400

gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat 2460

atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc 2520

gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac 2580

aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac 2640

tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc 2700

aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg 2760

gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact 2820

aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag 2880

ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac 2940

caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac 3000

cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg 3060

atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac 3120

atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct 3180

ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc 3240

accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag 3300

acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc 3360

agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat 3420

tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa 3480

gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt 3540

ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac 3600

tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag 3660

aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac 3720

tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag 3780

cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc 3840

ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc 3900

atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct 3960

gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag 4020

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac 4080

ctgtctcagc tgggaggtga ctctggcggc tcaaaaagaa ccgccgacgg cagcgaattc 4140

gagcccaaga agaagaggaa agtctaaccg gtcatcatca ccatcaccat tgagtttaaa 4200

cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 4260

ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 4320

aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 4380

acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 4440

tggcttctga ggcggaaaga accagctggg gctcgttgac agctagctca gtcctaggta 4500

taatactagt gctcttgccc ggcgtcaata cgttttagag ctagaaatag caagttaaaa 4560

taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgatccg 4620

gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta 4680

gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact 4740

atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 4800

tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 4860

tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 4920

tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 4980

gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 5040

agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 5100

cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 5160

agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag 5220

gtggcacttt tcggggaaat gtgggaaatg tgcgcggaac ccctatttgt ttatttttct 5280

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 5340

attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 5400

cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 5460

aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 5520

ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 5580

gtggcgcggt attatcccgt attgacgccg ggtaagagca actcggtcgc cgcatacact 5640

attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 5700

tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 5760

tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 5820

cccaagaaga agaggaaagt ctaaccggtc atcatcacca tcaccattga gtttaaaccc 5880

gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 5940

tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 6000

ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 6060

gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 6120

cttctgaggc ggaaagaacc agctggggct cgataccgtc gacctctagc tagagcttgg 6180

cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 6240

acatacgagc cggaagcata aagtgtaaag cctagggtgc ctaatgagtg agctaactca 6300

cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 6360

attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 6420

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 6480

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 6540

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 6600

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 6660

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 6720

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 6780

tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 6840

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 6900

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 6960

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 7020

gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 7080

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 7140

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 7200

ctacggggtc tgacactcag tggaacgaaa actcacgtta agggattttg gtcatgagat 7260

tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 7320

aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 7380

tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 7440

ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 7500

gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 7560

gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 7620

taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 7680

tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 7740

ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 7800

tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 7860

ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 7920

tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 7980

ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 8040

aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 8100

actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 8160

aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 8220

tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 8280

aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 8340

ctgacgtcga cggatcggga gatcgatctc ccgatcccct agggtcgact ctcagtacaa 8400

tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 8460

ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 8520

tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 8580

cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 8640

atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 8700

cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 8760

tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 8820

tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 8880

ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 8940

acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 9000

gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 9060

tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 9120

cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga 9180

accgtcagat ccgctagaga tccgcggccg ctaatacgac tcactatagg gagagccgcc 9240

acc 9243

<210> 36

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 36

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac ctctggagga tctagcggtg gttcctctgg aagcgagaca 2100

ccaggcacaa gcgagtccgc cacaccagag agctccggcg gctcctccgg aggatcctct 2160

gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc caagagggca 2220

tgggatgaaa gagaagtccc cgtgggcgcc gtgctggtgc acaacaatag agtgatcgga 2280

gagggatgga acaggccaat cggccgccac gaccctaccg cacacgcaga gatcatggca 2340

ctgaggcagg gaggcctggt catgcagaat taccgcctga tcgatgccac cctgtatgtg 2400

acactggagc catgcgtgat gtgcgcagga gcaatgatcc acagcaggat cggaagagtg 2460

gtgttcggag cacgggacgc caagaccggc gcagcaggct ccctgatgga tgtgctgcac 2520

caccccggca tgaaccaccg ggtggagatc acagagggaa tcctggcaga cgagtgcgcc 2580

gccctgctga gcgatttctt tagaatgcgg agacaggaga tcaaggccca gaagaaggca 2640

cagagctcca ccgactctgg aggatctagc ggcggatcct ctggaagcga gacaccaggc 2700

acaagcgagt ccgccacacc agagagctcc ggcggctcct ccggaggatc ctctgaggtg 2760

gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag ggcacgcgat 2820

gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat cggcgagggc 2880

tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat ggccctgaga 2940

cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta cgtgacattc 3000

gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg cgtggtgttt 3060

ggcgtgagga acgcaaaaac cggcgccgca ggctccctga tggacgtgct gcactacccc 3120

ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg tgccgccctg 3180

ctgtgctatt tctttcggat gcctagacag gtgttcaatg ctcagaagaa ggcccagagc 3240

tccaccgact ccggaggatc tagcggaggc tcctctggct ctgagacacc tggcacaagc 3300

gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcagaggg aatgagaaag 3360

cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac 3420

cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac 3480

tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat 3540

ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg 3600

gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg 3660

aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac 3720

accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc 3780

aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg 3840

atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag 3900

ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc 3960

atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc 4020

gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac 4080

agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg 4140

aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg 4200

cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac 4260

gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg 4320

ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg 4380

aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag 4440

ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc 4500

atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac 4560

tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc 4620

accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc 4680

gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc 4740

ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac 4800

gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac 4860

ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag 4920

atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag 4980

ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa 5040

aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc 5100

gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc 5160

cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa 5220

ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag 5280

aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc 5340

aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct 5400

gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 37

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 37

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

tctggaggat ctagcggtgg ttcctctgga agcgagacac caggcacaag cgagtccgcc 2280

acaccagaga gctccggcgg ctcctccgga ggatcctctg aggtggagtt ttcccacgag 2340

tactggatga gacatgccct gaccctggcc aagagggcat gggatgaaag agaagtcccc 2400

gtgggcgccg tgctggtgca caacaataga gtgatcggag agggatggaa caggccaatc 2460

ggccgccacg accctaccgc acacgcagag atcatggcac tgaggcaggg aggcctggtc 2520

atgcagaatt accgcctgat cgatgccacc ctgtatgtga cactggagcc atgcgtgatg 2580

tgcgcaggag caatgatcca cagcaggatc ggaagagtgg tgttcggagc acgggacgcc 2640

aagaccggcg cagcaggctc cctgatggat gtgctgcacc accccggcat gaaccaccgg 2700

gtggagatca cagagggaat cctggcagac gagtgcgccg ccctgctgag cgatttcttt 2760

agaatgcgga gacaggagat caaggcccag aagaaggcac agagctccac cgactctgga 2820

ggatctagcg gcggatcctc tggaagcgag acaccaggca caagcgagtc cgccacacca 2880

gagagctccg gcggctcctc cggaggatcc tctgaggtgg agttttccca cgagtactgg 2940

atgagacatg ccctgaccct ggccaagagg gcacgcgatg agagggaggt gcctgtggga 3000

gccgtgctgg tgctgaacaa tagagtgatc ggcgagggct ggaacagagc catcggcctg 3060

cacgacccaa cagcccatgc cgaaattatg gccctgagac agggcggcct ggtcatgcag 3120

aactacagac tgattgacgc caccctgtac gtgacattcg agccttgcgt gatgtgcgcc 3180

ggcgccatga tccactctag gatcggccgc gtggtgtttg gcgtgaggaa cgcaaaaacc 3240

ggcgccgcag gctccctgat ggacgtgctg cactaccccg gcatgaatca ccgcgtcgaa 3300

attaccgagg gaatcctggc agatgaatgt gccgccctgc tgtgctattt ctttcggatg 3360

cctagacagg tgttcaatgc tcagaagaag gcccagagct ccaccgactc cggaggatct 3420

agcggaggct cctctggctc tgagacacct ggcacaagcg agagcgcaac acctgaaagc 3480

agcgggggca gcagcggggg gtcagatcgg ttcaacgcct ccctgggcac ataccacgat 3540

ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg 3600

gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg 3660

aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac 3720

accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc 3780

aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg 3840

atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag 3900

ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc 3960

atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc 4020

gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac 4080

agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg 4140

aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg 4200

cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac 4260

gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg 4320

ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg 4380

aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag 4440

ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc 4500

atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac 4560

tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc 4620

accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc 4680

gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc 4740

ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac 4800

gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac 4860

ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag 4920

atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag 4980

ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa 5040

aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc 5100

gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc 5160

cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa 5220

ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag 5280

aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc 5340

aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct 5400

gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 38

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 38

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agtctggagg atctagcggt ggttcctctg gaagcgagac accaggcaca 2820

agcgagtccg ccacaccaga gagctccggc ggctcctccg gaggatcctc tgaggtggag 2880

ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc atgggatgaa 2940

agagaagtcc ccgtgggcgc cgtgctggtg cacaacaata gagtgatcgg agagggatgg 3000

aacaggccaa tcggccgcca cgaccctacc gcacacgcag agatcatggc actgaggcag 3060

ggaggcctgg tcatgcagaa ttaccgcctg atcgatgcca ccctgtatgt gacactggag 3120

ccatgcgtga tgtgcgcagg agcaatgatc cacagcagga tcggaagagt ggtgttcgga 3180

gcacgggacg ccaagaccgg cgcagcaggc tccctgatgg atgtgctgca ccaccccggc 3240

atgaaccacc gggtggagat cacagaggga atcctggcag acgagtgcgc cgccctgctg 3300

agcgatttct ttagaatgcg gagacaggag atcaaggccc agaagaaggc acagagctcc 3360

accgactctg gaggatctag cggcggatcc tctggaagcg agacaccagg cacaagcgag 3420

tccgccacac cagagagctc cggcggctcc tccggaggat cctctgaggt ggagttttcc 3480

cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacgcga tgagagggag 3540

gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg ctggaacaga 3600

gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag acagggcggc 3660

ctggtcatgc agaactacag actgattgac gccaccctgt acgtgacatt cgagccttgc 3720

gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt tggcgtgagg 3780

aacgcaaaaa ccggcgccgc aggctccctg atggacgtgc tgcactaccc cggcatgaat 3840

caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct gctgtgctat 3900

ttctttcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag ctccaccgac 3960

tccggaggat ctagcggagg ctcctctggc tctgagacac ctggcacaag cgagagcgca 4020

acacctgaaa gcagcggggg cagcagcggg gggtcaacca cccagaaggg acagaagaac 4080

agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg 4140

aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg 4200

cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac 4260

gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg 4320

ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg 4380

aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag 4440

ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc 4500

atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac 4560

tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc 4620

accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc 4680

gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc 4740

ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac 4800

gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac 4860

ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag 4920

atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag 4980

ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa 5040

aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc 5100

gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc 5160

cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa 5220

ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag 5280

aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc 5340

aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct 5400

gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 39

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 39

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaactctg gaggatctag cggtggttcc 2820

tctggaagcg agacaccagg cacaagcgag tccgccacac cagagagctc cggcggctcc 2880

tccggaggat cctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc 2940

ctggccaaga gggcatggga tgaaagagaa gtccccgtgg gcgccgtgct ggtgcacaac 3000

aatagagtga tcggagaggg atggaacagg ccaatcggcc gccacgaccc taccgcacac 3060

gcagagatca tggcactgag gcagggaggc ctggtcatgc agaattaccg cctgatcgat 3120

gccaccctgt atgtgacact ggagccatgc gtgatgtgcg caggagcaat gatccacagc 3180

aggatcggaa gagtggtgtt cggagcacgg gacgccaaga ccggcgcagc aggctccctg 3240

atggatgtgc tgcaccaccc cggcatgaac caccgggtgg agatcacaga gggaatcctg 3300

gcagacgagt gcgccgccct gctgagcgat ttctttagaa tgcggagaca ggagatcaag 3360

gcccagaaga aggcacagag ctccaccgac tctggaggat ctagcggcgg atcctctgga 3420

agcgagacac caggcacaag cgagtccgcc acaccagaga gctccggcgg ctcctccgga 3480

ggatcctctg aggtggagtt ttcccacgag tactggatga gacatgccct gaccctggcc 3540

aagagggcac gcgatgagag ggaggtgcct gtgggagccg tgctggtgct gaacaataga 3600

gtgatcggcg agggctggaa cagagccatc ggcctgcacg acccaacagc ccatgccgaa 3660

attatggccc tgagacaggg cggcctggtc atgcagaact acagactgat tgacgccacc 3720

ctgtacgtga cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcca ctctaggatc 3780

ggccgcgtgg tgtttggcgt gaggaacgca aaaaccggcg ccgcaggctc cctgatggac 3840

gtgctgcact accccggcat gaatcaccgc gtcgaaatta ccgagggaat cctggcagat 3900

gaatgtgccg ccctgctgtg ctatttcttt cggatgccta gacaggtgtt caatgctcag 3960

aagaaggccc agagctccac cgactccgga ggatctagcg gaggctcctc tggctctgag 4020

acacctggca caagcgagag cgcaacacct gaaagcagcg ggggcagcag cggggggtca 4080

agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg 4140

aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg 4200

cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac 4260

gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg 4320

ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg 4380

aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag 4440

ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc 4500

atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac 4560

tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc 4620

accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc 4680

gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc 4740

ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac 4800

gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac 4860

ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag 4920

atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag 4980

ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa 5040

aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc 5100

gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc 5160

cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa 5220

ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag 5280

aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc 5340

aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct 5400

gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 40

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 40

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggtctgg aggatctagc 2940

ggtggttcct ctggaagcga gacaccaggc acaagcgagt ccgccacacc agagagctcc 3000

ggcggctcct ccggaggatc ctctgaggtg gagttttccc acgagtactg gatgagacat 3060

gccctgaccc tggccaagag ggcatgggat gaaagagaag tccccgtggg cgccgtgctg 3120

gtgcacaaca atagagtgat cggagaggga tggaacaggc caatcggccg ccacgaccct 3180

accgcacacg cagagatcat ggcactgagg cagggaggcc tggtcatgca gaattaccgc 3240

ctgatcgatg ccaccctgta tgtgacactg gagccatgcg tgatgtgcgc aggagcaatg 3300

atccacagca ggatcggaag agtggtgttc ggagcacggg acgccaagac cggcgcagca 3360

ggctccctga tggatgtgct gcaccacccc ggcatgaacc accgggtgga gatcacagag 3420

ggaatcctgg cagacgagtg cgccgccctg ctgagcgatt tctttagaat gcggagacag 3480

gagatcaagg cccagaagaa ggcacagagc tccaccgact ctggaggatc tagcggcgga 3540

tcctctggaa gcgagacacc aggcacaagc gagtccgcca caccagagag ctccggcggc 3600

tcctccggag gatcctctga ggtggagttt tcccacgagt actggatgag acatgccctg 3660

accctggcca agagggcacg cgatgagagg gaggtgcctg tgggagccgt gctggtgctg 3720

aacaatagag tgatcggcga gggctggaac agagccatcg gcctgcacga cccaacagcc 3780

catgccgaaa ttatggccct gagacagggc ggcctggtca tgcagaacta cagactgatt 3840

gacgccaccc tgtacgtgac attcgagcct tgcgtgatgt gcgccggcgc catgatccac 3900

tctaggatcg gccgcgtggt gtttggcgtg aggaacgcaa aaaccggcgc cgcaggctcc 3960

ctgatggacg tgctgcacta ccccggcatg aatcaccgcg tcgaaattac cgagggaatc 4020

ctggcagatg aatgtgccgc cctgctgtgc tatttctttc ggatgcctag acaggtgttc 4080

aatgctcaga agaaggccca gagctccacc gactccggag gatctagcgg aggctcctct 4140

ggctctgaga cacctggcac aagcgagagc gcaacacctg aaagcagcgg gggcagcagc 4200

ggggggtcac gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac 4260

gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg 4320

ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg 4380

aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag 4440

ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc 4500

atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac 4560

tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc 4620

accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc 4680

gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc 4740

ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac 4800

gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac 4860

ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag 4920

atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag 4980

ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa 5040

aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc 5100

gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc 5160

cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa 5220

ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag 5280

aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc 5340

aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct 5400

gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 41

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 41

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ctctggagga tctagcggtg gttcctctgg aagcgagaca 3000

ccaggcacaa gcgagtccgc cacaccagag agctccggcg gctcctccgg aggatcctct 3060

gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc caagagggca 3120

tgggatgaaa gagaagtccc cgtgggcgcc gtgctggtgc acaacaatag agtgatcgga 3180

gagggatgga acaggccaat cggccgccac gaccctaccg cacacgcaga gatcatggca 3240

ctgaggcagg gaggcctggt catgcagaat taccgcctga tcgatgccac cctgtatgtg 3300

acactggagc catgcgtgat gtgcgcagga gcaatgatcc acagcaggat cggaagagtg 3360

gtgttcggag cacgggacgc caagaccggc gcagcaggct ccctgatgga tgtgctgcac 3420

caccccggca tgaaccaccg ggtggagatc acagagggaa tcctggcaga cgagtgcgcc 3480

gccctgctga gcgatttctt tagaatgcgg agacaggaga tcaaggccca gaagaaggca 3540

cagagctcca ccgactctgg aggatctagc ggcggatcct ctggaagcga gacaccaggc 3600

acaagcgagt ccgccacacc agagagctcc ggcggctcct ccggaggatc ctctgaggtg 3660

gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag ggcacgcgat 3720

gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat cggcgagggc 3780

tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat ggccctgaga 3840

cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta cgtgacattc 3900

gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg cgtggtgttt 3960

ggcgtgagga acgcaaaaac cggcgccgca ggctccctga tggacgtgct gcactacccc 4020

ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg tgccgccctg 4080

ctgtgctatt tctttcggat gcctagacag gtgttcaatg ctcagaagaa ggcccagagc 4140

tccaccgact ccggaggatc tagcggaggc tcctctggct ctgagacacc tggcacaagc 4200

gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcacggct gtccgactac 4260

gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg 4320

ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg 4380

aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag 4440

ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc 4500

atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac 4560

tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc 4620

accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc 4680

gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc 4740

ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac 4800

gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac 4860

ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag 4920

atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag 4980

ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa 5040

aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc 5100

gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc 5160

cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa 5220

ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag 5280

aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc 5340

aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct 5400

gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 42

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 42

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

tctggaggat ctagcggtgg ttcctctgga agcgagacac caggcacaag cgagtccgcc 3300

acaccagaga gctccggcgg ctcctccgga ggatcctctg aggtggagtt ttcccacgag 3360

tactggatga gacatgccct gaccctggcc aagagggcat gggatgaaag agaagtcccc 3420

gtgggcgccg tgctggtgca caacaataga gtgatcggag agggatggaa caggccaatc 3480

ggccgccacg accctaccgc acacgcagag atcatggcac tgaggcaggg aggcctggtc 3540

atgcagaatt accgcctgat cgatgccacc ctgtatgtga cactggagcc atgcgtgatg 3600

tgcgcaggag caatgatcca cagcaggatc ggaagagtgg tgttcggagc acgggacgcc 3660

aagaccggcg cagcaggctc cctgatggat gtgctgcacc accccggcat gaaccaccgg 3720

gtggagatca cagagggaat cctggcagac gagtgcgccg ccctgctgag cgatttcttt 3780

agaatgcgga gacaggagat caaggcccag aagaaggcac agagctccac cgactctgga 3840

ggatctagcg gcggatcctc tggaagcgag acaccaggca caagcgagtc cgccacacca 3900

gagagctccg gcggctcctc cggaggatcc tctgaggtgg agttttccca cgagtactgg 3960

atgagacatg ccctgaccct ggccaagagg gcacgcgatg agagggaggt gcctgtggga 4020

gccgtgctgg tgctgaacaa tagagtgatc ggcgagggct ggaacagagc catcggcctg 4080

cacgacccaa cagcccatgc cgaaattatg gccctgagac agggcggcct ggtcatgcag 4140

aactacagac tgattgacgc caccctgtac gtgacattcg agccttgcgt gatgtgcgcc 4200

ggcgccatga tccactctag gatcggccgc gtggtgtttg gcgtgaggaa cgcaaaaacc 4260

ggcgccgcag gctccctgat ggacgtgctg cactaccccg gcatgaatca ccgcgtcgaa 4320

attaccgagg gaatcctggc agatgaatgt gccgccctgc tgtgctattt ctttcggatg 4380

cctagacagg tgttcaatgc tcagaagaag gcccagagct ccaccgactc cggaggatct 4440

agcggaggct cctctggctc tgagacacct ggcacaagcg agagcgcaac acctgaaagc 4500

agcgggggca gcagcggggg gtcacggcag atcacaaagc acgtggcaca gatcctggac 4560

tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc 4620

accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc 4680

gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc 4740

ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac 4800

gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac 4860

ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag 4920

atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag 4980

ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa 5040

aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc 5100

gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc 5160

cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa 5220

ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag 5280

aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc 5340

aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct 5400

gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 43

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 43

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtctgg aggatctagc ggtggttcct ctggaagcga gacaccaggc 3540

acaagcgagt ccgccacacc agagagctcc ggcggctcct ccggaggatc ctctgaggtg 3600

gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag ggcatgggat 3660

gaaagagaag tccccgtggg cgccgtgctg gtgcacaaca atagagtgat cggagaggga 3720

tggaacaggc caatcggccg ccacgaccct accgcacacg cagagatcat ggcactgagg 3780

cagggaggcc tggtcatgca gaattaccgc ctgatcgatg ccaccctgta tgtgacactg 3840

gagccatgcg tgatgtgcgc aggagcaatg atccacagca ggatcggaag agtggtgttc 3900

ggagcacggg acgccaagac cggcgcagca ggctccctga tggatgtgct gcaccacccc 3960

ggcatgaacc accgggtgga gatcacagag ggaatcctgg cagacgagtg cgccgccctg 4020

ctgagcgatt tctttagaat gcggagacag gagatcaagg cccagaagaa ggcacagagc 4080

tccaccgact ctggaggatc tagcggcgga tcctctggaa gcgagacacc aggcacaagc 4140

gagtccgcca caccagagag ctccggcggc tcctccggag gatcctctga ggtggagttt 4200

tcccacgagt actggatgag acatgccctg accctggcca agagggcacg cgatgagagg 4260

gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac 4320

agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc 4380

ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct 4440

tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg 4500

aggaacgcaa aaaccggcgc cgcaggctcc ctgatggacg tgctgcacta ccccggcatg 4560

aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc 4620

tatttctttc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccacc 4680

gactccggag gatctagcgg aggctcctct ggctctgaga cacctggcac aagcgagagc 4740

gcaacacctg aaagcagcgg gggcagcagc ggggggtcat acggcgacta caaggtgtac 4800

gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac 4860

ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag 4920

atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag 4980

ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa 5040

aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc 5100

gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc 5160

cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa 5220

ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag 5280

aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc 5340

aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct 5400

gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 44

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 44

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgtctggagg atctagcggt 3540

ggttcctctg gaagcgagac accaggcaca agcgagtccg ccacaccaga gagctccggc 3600

ggctcctccg gaggatcctc tgaggtggag ttttcccacg agtactggat gagacatgcc 3660

ctgaccctgg ccaagagggc atgggatgaa agagaagtcc ccgtgggcgc cgtgctggtg 3720

cacaacaata gagtgatcgg agagggatgg aacaggccaa tcggccgcca cgaccctacc 3780

gcacacgcag agatcatggc actgaggcag ggaggcctgg tcatgcagaa ttaccgcctg 3840

atcgatgcca ccctgtatgt gacactggag ccatgcgtga tgtgcgcagg agcaatgatc 3900

cacagcagga tcggaagagt ggtgttcgga gcacgggacg ccaagaccgg cgcagcaggc 3960

tccctgatgg atgtgctgca ccaccccggc atgaaccacc gggtggagat cacagaggga 4020

atcctggcag acgagtgcgc cgccctgctg agcgatttct ttagaatgcg gagacaggag 4080

atcaaggccc agaagaaggc acagagctcc accgactctg gaggatctag cggcggatcc 4140

tctggaagcg agacaccagg cacaagcgag tccgccacac cagagagctc cggcggctcc 4200

tccggaggat cctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc 4260

ctggccaaga gggcacgcga tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac 4320

aatagagtga tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc aacagcccat 4380

gccgaaatta tggccctgag acagggcggc ctggtcatgc agaactacag actgattgac 4440

gccaccctgt acgtgacatt cgagccttgc gtgatgtgcg ccggcgccat gatccactct 4500

aggatcggcc gcgtggtgtt tggcgtgagg aacgcaaaaa ccggcgccgc aggctccctg 4560

atggacgtgc tgcactaccc cggcatgaat caccgcgtcg aaattaccga gggaatcctg 4620

gcagatgaat gtgccgccct gctgtgctat ttctttcgga tgcctagaca ggtgttcaat 4680

gctcagaaga aggcccagag ctccaccgac tccggaggat ctagcggagg ctcctctggc 4740

tctgagacac ctggcacaag cgagagcgca acacctgaaa gcagcggggg cagcagcggg 4800

gggtcacgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac 4860

ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag 4920

atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag 4980

ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa 5040

aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc 5100

gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc 5160

cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa 5220

ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag 5280

aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc 5340

aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct 5400

gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 45

<211> 8868

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 45

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga cctctggagg atctagcggt ggttcctctg gaagcgagac accaggcaca 3660

agcgagtccg ccacaccaga gagctccggc ggctcctccg gaggatcctc tgaggtggag 3720

ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc atgggatgaa 3780

agagaagtcc ccgtgggcgc cgtgctggtg cacaacaata gagtgatcgg agagggatgg 3840

aacaggccaa tcggccgcca cgaccctacc gcacacgcag agatcatggc actgaggcag 3900

ggaggcctgg tcatgcagaa ttaccgcctg atcgatgcca ccctgtatgt gacactggag 3960

ccatgcgtga tgtgcgcagg agcaatgatc cacagcagga tcggaagagt ggtgttcgga 4020

gcacgggacg ccaagaccgg cgcagcaggc tccctgatgg atgtgctgca ccaccccggc 4080

atgaaccacc gggtggagat cacagaggga atcctggcag acgagtgcgc cgccctgctg 4140

agcgatttct ttagaatgcg gagacaggag atcaaggccc agaagaaggc acagagctcc 4200

accgactctg gaggatctag cggcggatcc tctggaagcg agacaccagg cacaagcgag 4260

tccgccacac cagagagctc cggcggctcc tccggaggat cctctgaggt ggagttttcc 4320

cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacgcga tgagagggag 4380

gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg ctggaacaga 4440

gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag acagggcggc 4500

ctggtcatgc agaactacag actgattgac gccaccctgt acgtgacatt cgagccttgc 4560

gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt tggcgtgagg 4620

aacgcaaaaa ccggcgccgc aggctccctg atggacgtgc tgcactaccc cggcatgaat 4680

caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct gctgtgctat 4740

ttctttcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag ctccaccgac 4800

tccggaggat ctagcggagg ctcctctggc tctgagacac ctggcacaag cgagagcgca 4860

acacctgaaa gcagcggggg cagcagcggg gggtcagaga caaacggcga aaccggggag 4920

atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa 4980

gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg 5040

cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac 5100

ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag 5160

ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga 5220

agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa 5280

aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag 5340

agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa 5400

tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat 5460

aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag 5520

cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg 5580

tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac 5640

ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc 5700

gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc 5760

atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggtga ctctggcggc 5820

tcaaaaagaa ccgccgacgg cagcgaattc gagcccaaga agaagaggaa agtctaaccg 5880

gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta 5940

gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 6000

ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 6060

attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 6120

gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg 6180

gctcgatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 6240

tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 6300

aagcctaggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 6360

ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6420

gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6480

tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6540

aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6600

gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6660

aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6720

ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6780

tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6840

tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6900

ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 6960

tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 7020

ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 7080

tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 7140

aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 7200

aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacact cagtggaacg 7260

aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 7320

ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 7380

acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 7440

ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 7500

gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 7560

taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 7620

tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 7680

gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 7740

cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 7800

aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 7860

cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 7920

tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 7980

gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 8040

tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 8100

gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 8160

ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 8220

cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 8280

agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 8340

gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat 8400

ctcccgatcc cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc 8460

agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag 8520

ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt 8580

ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt 8640

attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta 8700

cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 8760

caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg 8820

tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatc 8868

<210> 46

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 46

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 3660

acaaacggcg aaaccgggga gatctctgga ggatctagcg gtggttcctc tggaagcgag 3720

acaccaggca caagcgagtc cgccacacca gagagctccg gcggctcctc cggaggatcc 3780

tctgaggtgg agttttccca cgagtactgg atgagacatg ccctgaccct ggccaagagg 3840

gcatgggatg aaagagaagt ccccgtgggc gccgtgctgg tgcacaacaa tagagtgatc 3900

ggagagggat ggaacaggcc aatcggccgc cacgacccta ccgcacacgc agagatcatg 3960

gcactgaggc agggaggcct ggtcatgcag aattaccgcc tgatcgatgc caccctgtat 4020

gtgacactgg agccatgcgt gatgtgcgca ggagcaatga tccacagcag gatcggaaga 4080

gtggtgttcg gagcacggga cgccaagacc ggcgcagcag gctccctgat ggatgtgctg 4140

caccaccccg gcatgaacca ccgggtggag atcacagagg gaatcctggc agacgagtgc 4200

gccgccctgc tgagcgattt ctttagaatg cggagacagg agatcaaggc ccagaagaag 4260

gcacagagct ccaccgactc tggaggatct agcggcggat cctctggaag cgagacacca 4320

ggcacaagcg agtccgccac accagagagc tccggcggct cctccggagg atcctctgag 4380

gtggagtttt cccacgagta ctggatgaga catgccctga ccctggccaa gagggcacgc 4440

gatgagaggg aggtgcctgt gggagccgtg ctggtgctga acaatagagt gatcggcgag 4500

ggctggaaca gagccatcgg cctgcacgac ccaacagccc atgccgaaat tatggccctg 4560

agacagggcg gcctggtcat gcagaactac agactgattg acgccaccct gtacgtgaca 4620

ttcgagcctt gcgtgatgtg cgccggcgcc atgatccact ctaggatcgg ccgcgtggtg 4680

tttggcgtga ggaacgcaaa aaccggcgcc gcaggctccc tgatggacgt gctgcactac 4740

cccggcatga atcaccgcgt cgaaattacc gagggaatcc tggcagatga atgtgccgcc 4800

ctgctgtgct atttctttcg gatgcctaga caggtgttca atgctcagaa gaaggcccag 4860

agctccaccg actccggagg atctagcgga ggctcctctg gctctgagac acctggcaca 4920

agcgagagcg caacacctga aagcagcggg ggcagcagcg gggggtcagt gtgggataag 4980

ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa 5040

aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc 5100

gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc 5160

cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa 5220

ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag 5280

aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc 5340

aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct 5400

gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 47

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 47

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 3660

acaaacggcg aaaccgggga gatcgtgtct ggaggatcta gcggtggttc ctctggaagc 3720

gagacaccag gcacaagcga gtccgccaca ccagagagct ccggcggctc ctccggagga 3780

tcctctgagg tggagttttc ccacgagtac tggatgagac atgccctgac cctggccaag 3840

agggcatggg atgaaagaga agtccccgtg ggcgccgtgc tggtgcacaa caatagagtg 3900

atcggagagg gatggaacag gccaatcggc cgccacgacc ctaccgcaca cgcagagatc 3960

atggcactga ggcagggagg cctggtcatg cagaattacc gcctgatcga tgccaccctg 4020

tatgtgacac tggagccatg cgtgatgtgc gcaggagcaa tgatccacag caggatcgga 4080

agagtggtgt tcggagcacg ggacgccaag accggcgcag caggctccct gatggatgtg 4140

ctgcaccacc ccggcatgaa ccaccgggtg gagatcacag agggaatcct ggcagacgag 4200

tgcgccgccc tgctgagcga tttctttaga atgcggagac aggagatcaa ggcccagaag 4260

aaggcacaga gctccaccga ctctggagga tctagcggcg gatcctctgg aagcgagaca 4320

ccaggcacaa gcgagtccgc cacaccagag agctccggcg gctcctccgg aggatcctct 4380

gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc caagagggca 4440

cgcgatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc 4500

gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga aattatggcc 4560

ctgagacagg gcggcctggt catgcagaac tacagactga ttgacgccac cctgtacgtg 4620

acattcgagc cttgcgtgat gtgcgccggc gccatgatcc actctaggat cggccgcgtg 4680

gtgtttggcg tgaggaacgc aaaaaccggc gccgcaggct ccctgatgga cgtgctgcac 4740

taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga tgaatgtgcc 4800

gccctgctgt gctatttctt tcggatgcct agacaggtgt tcaatgctca gaagaaggcc 4860

cagagctcca ccgactccgg aggatctagc ggaggctcct ctggctctga gacacctggc 4920

acaagcgaga gcgcaacacc tgaaagcagc gggggcagca gcggggggtc atgggataag 4980

ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa 5040

aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc 5100

gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc 5160

cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa 5220

ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag 5280

aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc 5340

aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct 5400

gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 48

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 48

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 3660

acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg 3720

aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc 3780

ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag 3840

gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg 3900

gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg 3960

gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc 4020

aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc 4080

gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac 4140

gaactggcct ctggaggatc tagcggtggt tcctctggaa gcgagacacc aggcacaagc 4200

gagtccgcca caccagagag ctccggcggc tcctccggag gatcctctga ggtggagttt 4260

tcccacgagt actggatgag acatgccctg accctggcca agagggcatg ggatgaaaga 4320

gaagtccccg tgggcgccgt gctggtgcac aacaatagag tgatcggaga gggatggaac 4380

aggccaatcg gccgccacga ccctaccgca cacgcagaga tcatggcact gaggcaggga 4440

ggcctggtca tgcagaatta ccgcctgatc gatgccaccc tgtatgtgac actggagcca 4500

tgcgtgatgt gcgcaggagc aatgatccac agcaggatcg gaagagtggt gttcggagca 4560

cgggacgcca agaccggcgc agcaggctcc ctgatggatg tgctgcacca ccccggcatg 4620

aaccaccggg tggagatcac agagggaatc ctggcagacg agtgcgccgc cctgctgagc 4680

gatttcttta gaatgcggag acaggagatc aaggcccaga agaaggcaca gagctccacc 4740

gactctggag gatctagcgg cggatcctct ggaagcgaga caccaggcac aagcgagtcc 4800

gccacaccag agagctccgg cggctcctcc ggaggatcct ctgaggtgga gttttcccac 4860

gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgcgatga gagggaggtg 4920

cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc 4980

atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg 5040

gtcatgcaga actacagact gattgacgcc accctgtacg tgacattcga gccttgcgtg 5100

atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac 5160

gcaaaaaccg gcgccgcagg ctccctgatg gacgtgctgc actaccccgg catgaatcac 5220

cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgctatttc 5280

tttcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc caccgactcc 5340

ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga gagcgcaaca 5400

cctgaaagca gcgggggcag cagcgggggg tcactgccct ccaaatatgt gaacttcctg 5460

tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 49

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 49

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 3660

acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg 3720

aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc 3780

ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag 3840

gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg 3900

gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg 3960

gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc 4020

aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc 4080

gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac 4140

gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag 4200

ctgaagtctg gaggatctag cggtggttcc tctggaagcg agacaccagg cacaagcgag 4260

tccgccacac cagagagctc cggcggctcc tccggaggat cctctgaggt ggagttttcc 4320

cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcatggga tgaaagagaa 4380

gtccccgtgg gcgccgtgct ggtgcacaac aatagagtga tcggagaggg atggaacagg 4440

ccaatcggcc gccacgaccc taccgcacac gcagagatca tggcactgag gcagggaggc 4500

ctggtcatgc agaattaccg cctgatcgat gccaccctgt atgtgacact ggagccatgc 4560

gtgatgtgcg caggagcaat gatccacagc aggatcggaa gagtggtgtt cggagcacgg 4620

gacgccaaga ccggcgcagc aggctccctg atggatgtgc tgcaccaccc cggcatgaac 4680

caccgggtgg agatcacaga gggaatcctg gcagacgagt gcgccgccct gctgagcgat 4740

ttctttagaa tgcggagaca ggagatcaag gcccagaaga aggcacagag ctccaccgac 4800

tctggaggat ctagcggcgg atcctctgga agcgagacac caggcacaag cgagtccgcc 4860

acaccagaga gctccggcgg ctcctccgga ggatcctctg aggtggagtt ttcccacgag 4920

tactggatga gacatgccct gaccctggcc aagagggcac gcgatgagag ggaggtgcct 4980

gtgggagccg tgctggtgct gaacaataga gtgatcggcg agggctggaa cagagccatc 5040

ggcctgcacg acccaacagc ccatgccgaa attatggccc tgagacaggg cggcctggtc 5100

atgcagaact acagactgat tgacgccacc ctgtacgtga cattcgagcc ttgcgtgatg 5160

tgcgccggcg ccatgatcca ctctaggatc ggccgcgtgg tgtttggcgt gaggaacgca 5220

aaaaccggcg ccgcaggctc cctgatggac gtgctgcact accccggcat gaatcaccgc 5280

gtcgaaatta ccgagggaat cctggcagat gaatgtgccg ccctgctgtg ctatttcttt 5340

cggatgccta gacaggtgtt caatgctcag aagaaggccc agagctccac cgactccgga 5400

ggatctagcg gaggctcctc tggctctgag acacctggca caagcgagag cgcaacacct 5460

gaaagcagcg ggggcagcag cggggggtca ggctcccccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 50

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 50

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 3660

acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg 3720

aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc 3780

ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag 3840

gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg 3900

gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg 3960

gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc 4020

aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc 4080

gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac 4140

gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag 4200

ctgaagggct cctctggagg atctagcggt ggttcctctg gaagcgagac accaggcaca 4260

agcgagtccg ccacaccaga gagctccggc ggctcctccg gaggatcctc tgaggtggag 4320

ttttcccacg agtactggat gagacatgcc ctgaccctgg ccaagagggc atgggatgaa 4380

agagaagtcc ccgtgggcgc cgtgctggtg cacaacaata gagtgatcgg agagggatgg 4440

aacaggccaa tcggccgcca cgaccctacc gcacacgcag agatcatggc actgaggcag 4500

ggaggcctgg tcatgcagaa ttaccgcctg atcgatgcca ccctgtatgt gacactggag 4560

ccatgcgtga tgtgcgcagg agcaatgatc cacagcagga tcggaagagt ggtgttcgga 4620

gcacgggacg ccaagaccgg cgcagcaggc tccctgatgg atgtgctgca ccaccccggc 4680

atgaaccacc gggtggagat cacagaggga atcctggcag acgagtgcgc cgccctgctg 4740

agcgatttct ttagaatgcg gagacaggag atcaaggccc agaagaaggc acagagctcc 4800

accgactctg gaggatctag cggcggatcc tctggaagcg agacaccagg cacaagcgag 4860

tccgccacac cagagagctc cggcggctcc tccggaggat cctctgaggt ggagttttcc 4920

cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacgcga tgagagggag 4980

gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg ctggaacaga 5040

gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag acagggcggc 5100

ctggtcatgc agaactacag actgattgac gccaccctgt acgtgacatt cgagccttgc 5160

gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt tggcgtgagg 5220

aacgcaaaaa ccggcgccgc aggctccctg atggacgtgc tgcactaccc cggcatgaat 5280

caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct gctgtgctat 5340

ttctttcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag ctccaccgac 5400

tccggaggat ctagcggagg ctcctctggc tctgagacac ctggcacaag cgagagcgca 5460

acacctgaaa gcagcggggg cagcagcggg gggtcacccg aggataatga gcagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 51

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 51

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 3660

acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg 3720

aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc 3780

ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag 3840

gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg 3900

gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg 3960

gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc 4020

aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc 4080

gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac 4140

gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag 4200

ctgaagggct cccccgagga taatgagtct ggaggatcta gcggtggttc ctctggaagc 4260

gagacaccag gcacaagcga gtccgccaca ccagagagct ccggcggctc ctccggagga 4320

tcctctgagg tggagttttc ccacgagtac tggatgagac atgccctgac cctggccaag 4380

agggcatggg atgaaagaga agtccccgtg ggcgccgtgc tggtgcacaa caatagagtg 4440

atcggagagg gatggaacag gccaatcggc cgccacgacc ctaccgcaca cgcagagatc 4500

atggcactga ggcagggagg cctggtcatg cagaattacc gcctgatcga tgccaccctg 4560

tatgtgacac tggagccatg cgtgatgtgc gcaggagcaa tgatccacag caggatcgga 4620

agagtggtgt tcggagcacg ggacgccaag accggcgcag caggctccct gatggatgtg 4680

ctgcaccacc ccggcatgaa ccaccgggtg gagatcacag agggaatcct ggcagacgag 4740

tgcgccgccc tgctgagcga tttctttaga atgcggagac aggagatcaa ggcccagaag 4800

aaggcacaga gctccaccga ctctggagga tctagcggcg gatcctctgg aagcgagaca 4860

ccaggcacaa gcgagtccgc cacaccagag agctccggcg gctcctccgg aggatcctct 4920

gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc caagagggca 4980

cgcgatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag agtgatcggc 5040

gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga aattatggcc 5100

ctgagacagg gcggcctggt catgcagaac tacagactga ttgacgccac cctgtacgtg 5160

acattcgagc cttgcgtgat gtgcgccggc gccatgatcc actctaggat cggccgcgtg 5220

gtgtttggcg tgaggaacgc aaaaaccggc gccgcaggct ccctgatgga cgtgctgcac 5280

taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga tgaatgtgcc 5340

gccctgctgt gctatttctt tcggatgcct agacaggtgt tcaatgctca gaagaaggcc 5400

cagagctcca ccgactccgg aggatctagc ggaggctcct ctggctctga gacacctggc 5460

acaagcgaga gcgcaacacc tgaaagcagc gggggcagca gcggggggtc acagaaacag 5520

ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 52

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 52

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 3660

acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg 3720

aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc 3780

ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag 3840

gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg 3900

gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg 3960

gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc 4020

aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc 4080

gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac 4140

gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag 4200

ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaatc tggaggatct 4260

agcggtggtt cctctggaag cgagacacca ggcacaagcg agtccgccac accagagagc 4320

tccggcggct cctccggagg atcctctgag gtggagtttt cccacgagta ctggatgaga 4380

catgccctga ccctggccaa gagggcatgg gatgaaagag aagtccccgt gggcgccgtg 4440

ctggtgcaca acaatagagt gatcggagag ggatggaaca ggccaatcgg ccgccacgac 4500

cctaccgcac acgcagagat catggcactg aggcagggag gcctggtcat gcagaattac 4560

cgcctgatcg atgccaccct gtatgtgaca ctggagccat gcgtgatgtg cgcaggagca 4620

atgatccaca gcaggatcgg aagagtggtg ttcggagcac gggacgccaa gaccggcgca 4680

gcaggctccc tgatggatgt gctgcaccac cccggcatga accaccgggt ggagatcaca 4740

gagggaatcc tggcagacga gtgcgccgcc ctgctgagcg atttctttag aatgcggaga 4800

caggagatca aggcccagaa gaaggcacag agctccaccg actctggagg atctagcggc 4860

ggatcctctg gaagcgagac accaggcaca agcgagtccg ccacaccaga gagctccggc 4920

ggctcctccg gaggatcctc tgaggtggag ttttcccacg agtactggat gagacatgcc 4980

ctgaccctgg ccaagagggc acgcgatgag agggaggtgc ctgtgggagc cgtgctggtg 5040

ctgaacaata gagtgatcgg cgagggctgg aacagagcca tcggcctgca cgacccaaca 5100

gcccatgccg aaattatggc cctgagacag ggcggcctgg tcatgcagaa ctacagactg 5160

attgacgcca ccctgtacgt gacattcgag ccttgcgtga tgtgcgccgg cgccatgatc 5220

cactctagga tcggccgcgt ggtgtttggc gtgaggaacg caaaaaccgg cgccgcaggc 5280

tccctgatgg acgtgctgca ctaccccggc atgaatcacc gcgtcgaaat taccgaggga 5340

atcctggcag atgaatgtgc cgccctgctg tgctatttct ttcggatgcc tagacaggtg 5400

ttcaatgctc agaagaaggc ccagagctcc accgactccg gaggatctag cggaggctcc 5460

tctggctctg agacacctgg cacaagcgag agcgcaacac ctgaaagcag cgggggcagc 5520

agcggggggt cacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 53

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 53

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 3660

acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg 3720

aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc 3780

ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag 3840

gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg 3900

gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg 3960

gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc 4020

aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc 4080

gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac 4140

gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag 4200

ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagtct 4260

ggaggatcta gcggtggttc ctctggaagc gagacaccag gcacaagcga gtccgccaca 4320

ccagagagct ccggcggctc ctccggagga tcctctgagg tggagttttc ccacgagtac 4380

tggatgagac atgccctgac cctggccaag agggcatggg atgaaagaga agtccccgtg 4440

ggcgccgtgc tggtgcacaa caatagagtg atcggagagg gatggaacag gccaatcggc 4500

cgccacgacc ctaccgcaca cgcagagatc atggcactga ggcagggagg cctggtcatg 4560

cagaattacc gcctgatcga tgccaccctg tatgtgacac tggagccatg cgtgatgtgc 4620

gcaggagcaa tgatccacag caggatcgga agagtggtgt tcggagcacg ggacgccaag 4680

accggcgcag caggctccct gatggatgtg ctgcaccacc ccggcatgaa ccaccgggtg 4740

gagatcacag agggaatcct ggcagacgag tgcgccgccc tgctgagcga tttctttaga 4800

atgcggagac aggagatcaa ggcccagaag aaggcacaga gctccaccga ctctggagga 4860

tctagcggcg gatcctctgg aagcgagaca ccaggcacaa gcgagtccgc cacaccagag 4920

agctccggcg gctcctccgg aggatcctct gaggtggagt tttcccacga gtactggatg 4980

agacatgccc tgaccctggc caagagggca cgcgatgaga gggaggtgcc tgtgggagcc 5040

gtgctggtgc tgaacaatag agtgatcggc gagggctgga acagagccat cggcctgcac 5100

gacccaacag cccatgccga aattatggcc ctgagacagg gcggcctggt catgcagaac 5160

tacagactga ttgacgccac cctgtacgtg acattcgagc cttgcgtgat gtgcgccggc 5220

gccatgatcc actctaggat cggccgcgtg gtgtttggcg tgaggaacgc aaaaaccggc 5280

gccgcaggct ccctgatgga cgtgctgcac taccccggca tgaatcaccg cgtcgaaatt 5340

accgagggaa tcctggcaga tgaatgtgcc gccctgctgt gctatttctt tcggatgcct 5400

agacaggtgt tcaatgctca gaagaaggcc cagagctcca ccgactccgg aggatctagc 5460

ggaggctcct ctggctctga gacacctggc acaagcgaga gcgcaacacc tgaaagcagc 5520

gggggcagca gcggggggtc acactacctg gacgagatca tcgagcagat cagcgagttc 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 54

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 54

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 3660

acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg 3720

aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc 3780

ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag 3840

gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg 3900

gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg 3960

gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc 4020

aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc 4080

gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac 4140

gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag 4200

ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac 4260

tacctggacg agatcatcga gcagatcagc gagttctctg gaggatctag cggtggttcc 4320

tctggaagcg agacaccagg cacaagcgag tccgccacac cagagagctc cggcggctcc 4380

tccggaggat cctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc 4440

ctggccaaga gggcatggga tgaaagagaa gtccccgtgg gcgccgtgct ggtgcacaac 4500

aatagagtga tcggagaggg atggaacagg ccaatcggcc gccacgaccc taccgcacac 4560

gcagagatca tggcactgag gcagggaggc ctggtcatgc agaattaccg cctgatcgat 4620

gccaccctgt atgtgacact ggagccatgc gtgatgtgcg caggagcaat gatccacagc 4680

aggatcggaa gagtggtgtt cggagcacgg gacgccaaga ccggcgcagc aggctccctg 4740

atggatgtgc tgcaccaccc cggcatgaac caccgggtgg agatcacaga gggaatcctg 4800

gcagacgagt gcgccgccct gctgagcgat ttctttagaa tgcggagaca ggagatcaag 4860

gcccagaaga aggcacagag ctccaccgac tctggaggat ctagcggcgg atcctctgga 4920

agcgagacac caggcacaag cgagtccgcc acaccagaga gctccggcgg ctcctccgga 4980

ggatcctctg aggtggagtt ttcccacgag tactggatga gacatgccct gaccctggcc 5040

aagagggcac gcgatgagag ggaggtgcct gtgggagccg tgctggtgct gaacaataga 5100

gtgatcggcg agggctggaa cagagccatc ggcctgcacg acccaacagc ccatgccgaa 5160

attatggccc tgagacaggg cggcctggtc atgcagaact acagactgat tgacgccacc 5220

ctgtacgtga cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcca ctctaggatc 5280

ggccgcgtgg tgtttggcgt gaggaacgca aaaaccggcg ccgcaggctc cctgatggac 5340

gtgctgcact accccggcat gaatcaccgc gtcgaaatta ccgagggaat cctggcagat 5400

gaatgtgccg ccctgctgtg ctatttcttt cggatgccta gacaggtgtt caatgctcag 5460

aagaaggccc agagctccac cgactccgga ggatctagcg gaggctcctc tggctctgag 5520

acacctggca caagcgagag cgcaacacct gaaagcagcg ggggcagcag cggggggtca 5580

tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag 5640

caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 55

<211> 8913

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 55

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 120

ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 180

cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 240

atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 300

ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 360

agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 420

gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcagcag tgacaagaag 480

tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag 540

tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag 600

aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg 660

aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag 720

atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc 780

ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac 840

gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac 900

agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc 960

cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg 1020

ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc 1080

ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat 1140

ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg 1200

agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg 1260

cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac 1320

cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac 1380

atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga 1440

tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct 1500

gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac 1560

ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac 1620

ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc 1680

ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg 1740

cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg 1800

accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg 1860

atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag 1920

ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac 1980

gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg 2040

accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag 2100

aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg 2160

aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa 2220

gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag 2280

gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca 2340

ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac 2400

gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg 2460

aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag 2520

tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt 2580

aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt 2640

gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg 2700

gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc 2760

agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc 2820

gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc 2880

cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg 2940

gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag 3000

agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg 3060

ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg 3120

cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag 3180

agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc 3240

cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac 3300

gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc 3360

gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc 3420

cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg 3480

gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag 3540

agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac 3600

tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag 3660

acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg 3720

aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc 3780

ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag 3840

gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg 3900

gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg 3960

gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc 4020

aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc 4080

gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac 4140

gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag 4200

ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac 4260

tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac 4320

gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catctctgga 4380

ggatctagcg gtggttcctc tggaagcgag acaccaggca caagcgagtc cgccacacca 4440

gagagctccg gcggctcctc cggaggatcc tctgaggtgg agttttccca cgagtactgg 4500

atgagacatg ccctgaccct ggccaagagg gcatgggatg aaagagaagt ccccgtgggc 4560

gccgtgctgg tgcacaacaa tagagtgatc ggagagggat ggaacaggcc aatcggccgc 4620

cacgacccta ccgcacacgc agagatcatg gcactgaggc agggaggcct ggtcatgcag 4680

aattaccgcc tgatcgatgc caccctgtat gtgacactgg agccatgcgt gatgtgcgca 4740

ggagcaatga tccacagcag gatcggaaga gtggtgttcg gagcacggga cgccaagacc 4800

ggcgcagcag gctccctgat ggatgtgctg caccaccccg gcatgaacca ccgggtggag 4860

atcacagagg gaatcctggc agacgagtgc gccgccctgc tgagcgattt ctttagaatg 4920

cggagacagg agatcaaggc ccagaagaag gcacagagct ccaccgactc tggaggatct 4980

agcggcggat cctctggaag cgagacacca ggcacaagcg agtccgccac accagagagc 5040

tccggcggct cctccggagg atcctctgag gtggagtttt cccacgagta ctggatgaga 5100

catgccctga ccctggccaa gagggcacgc gatgagaggg aggtgcctgt gggagccgtg 5160

ctggtgctga acaatagagt gatcggcgag ggctggaaca gagccatcgg cctgcacgac 5220

ccaacagccc atgccgaaat tatggccctg agacagggcg gcctggtcat gcagaactac 5280

agactgattg acgccaccct gtacgtgaca ttcgagcctt gcgtgatgtg cgccggcgcc 5340

atgatccact ctaggatcgg ccgcgtggtg tttggcgtga ggaacgcaaa aaccggcgcc 5400

gcaggctccc tgatggacgt gctgcactac cccggcatga atcaccgcgt cgaaattacc 5460

gagggaatcc tggcagatga atgtgccgcc ctgctgtgct atttctttcg gatgcctaga 5520

caggtgttca atgctcagaa gaaggcccag agctccaccg actccggagg atctagcgga 5580

ggctcctctg gctctgagac acctggcaca agcgagagcg caacacctga aagcagcggg 5640

ggcagcagcg gggggtcaag agagcaggcc gagaatatca tccacctgtt taccctgacc 5700

aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac 5760

accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac 5820

gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc 5880

gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc 5940

accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 6000

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 6060

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 6120

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 6180

atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga 6240

cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6300

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct 6360

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6420

acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6480

ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6540

gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6600

caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6660

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6720

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6780

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6840

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6900

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 6960

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7020

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7080

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 7140

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7200

gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7260

aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag 7320

ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7380

gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7440

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7500

tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7560

tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7620

gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7680

gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7740

ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7800

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7860

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7920

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 7980

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8040

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8100

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8160

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8220

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8280

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8340

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8400

ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag 8460

ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 8520

cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 8580

gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg 8640

atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa 8700

ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 8760

atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 8820

ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 8880

aaactgccca cttggcagta catcaagtgt atc 8913

<210> 56

<211> 8924

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 56

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta ctggcagtac atctacgtat tagtcatcgc 120

tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 180

acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 240

tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 300

gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 360

gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg aaacggacag 420

ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcagcagt gacaagaagt 480

acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt 540

acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga 600

agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga 660

agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga 720

tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct 780

tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg 840

aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca 900

gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc 960

ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt 1020

tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg 1080

gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc 1140

tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga 1200

gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc 1260

agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc 1320

agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca 1380

tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat 1440

acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg 1500

agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg 1560

gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg 1620

gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct 1680

tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc 1740

ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga 1800

ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga 1860

tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg 1920

gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg 1980

agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga 2040

ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga 2100

aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga 2160

aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag 2220

atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg 2280

acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac 2340

tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg 2400

acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga 2460

agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt 2520

ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta 2580

aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg 2640

ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg 2700

acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca 2760

gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg 2820

aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc 2880

agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg 2940

accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga 3000

gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg 3060

gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc 3120

agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga 3180

gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc 3240

ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg 3300

agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg 3360

atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc 3420

acgacgccta cctaaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg 3480

aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga 3540

gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact 3600

ttttcaagtc cggatccgag accccaggca cctccgagtc tgccacacct gagagcggaa 3660

gcgaaaccgg accagtggca gtggacccaa ccctgaggag acggattgag ccccatgaat 3720

ttgaagtgtt ctttgaccca agggagctga ggaaggagac atgcctgctg tacgagatca 3780

agtggggcac aagccacaag atctggcgcc acagctccaa gaacaccaca aagcacgtgg 3840

aagtgaattt catcgagaag tttacctccg agcggcactt ctgcccctct accagctgtt 3900

ccatcacatg gtttctgtct tggagccctt gcggcgagtg ttccaaggcc atcaccgagt 3960

tcctgtctca gcaccctaac gtgaccctgg tcatctacgt ggcccggctg tatcaccaca 4020

tggaccagca gaacaggcag ggcctgcgcg atctggtgaa ttctggcgtg accatccaga 4080

tcatgacagc cccagagtac gactattgct ggcggaactt cgtgaattat ccacctggca 4140

aggaggcaca ctggccaaga tacccacccc tgtggatgaa gctgtatgca ctggagctgc 4200

acgcaggaat cctgggcctg cctccatgtc tgaatatcct gcggagaaag cagccccagc 4260

tgacattttt caccattgct ctgcagtctt gtcactatca gcggctgcct cctcatattc 4320

tgtgggctac aggcctgaag tctggatctg gcagcgagac accaggaaca agcgagtcag 4380

caacaccaga gagcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg 4440

attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg 4500

aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc 4560

tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg 4620

tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga 4680

gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca 4740

tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc 4800

ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg 4860

aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg 4920

ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg 4980

tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga 5040

gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg 5100

ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg 5160

gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca 5220

ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac 5280

ggatcgacct gtctcagctg ggaggtgaca gcggcgggag cggcgggagc ggggggagca 5340

ctaatctgag cgacatcatt gagaaggaga ctgggaaaca gctggtcatt caggagtcca 5400

tcctgatgct gcctgaggag gtggaggaag tgatcggcaa caagccagag tctgacatcc 5460

tggtgcacac cgcctacgac gagtccacag atgagaatgt gatgctgctg acctctgacg 5520

cccccgagta taagccttgg gccctggtca tccaggattc taacggcgag aataagatca 5580

agatgctgag cggaggatcc ggaggatctg gaggcagcac caacctgtct gacatcatcg 5640

agaaggagac aggcaagcag ctggtcatcc aggagagcat cctgatgctg cccgaagaag 5700

tcgaagaagt gatcggaaac aagcctgaga gcgatatcct ggtccatacc gcctacgacg 5760

agagtaccga cgaaaatgtg atgctgctga catccgacgc cccagagtat aagccctggg 5820

ctctggtcat ccaggattcc aacggagaga acaaaatcaa aatgctgtct ggcggctcaa 5880

aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc taaccggtca 5940

tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg 6000

ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 6060

cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 6120

tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 6180

gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 6240

gataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 6300

aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 6360

ctaggatgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 6420

ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cgggaagagg 6480

cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6540

tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6600

aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 6660

aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 6720

tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 6780

ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 6840

cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 6900

ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 6960

ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7020

gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7080

agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 7140

cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7200

aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7260

aggatctcaa gaagatcctt tgatcttttc tacggggtct gacactcagt ggaacgaaaa 7320

ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 7380

aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 7440

ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 7500

agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 7560

cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 7620

ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 7680

gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 7740

cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 7800

cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 7860

ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 7920

catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 7980

tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 8040

ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 8100

catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 8160

cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 8220

cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 8280

acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 8340

ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 8400

tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc 8460

cgatccccta gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta 8520

tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac 8580

aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc 8640

gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta 8700

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 8760

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 8820

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 8880

gtatttacgg taaactgccc acttggcagt acatcaagtg tatc 8924

<210> 57

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 57

ccttcccaga aaacctacca ggg 23

<210> 58

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 58

caacccccag agcacggtgg tgg 23

<210> 59

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 59

caaatctgtc acattgggta agg 23

<210> 60

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 60

acagctgcag agagccctgc agg 23

<210> 61

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 61

ttccgcctcc gacctgtggc tgg 23

<210> 62

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 62

ttccttcagg ctctgaatct tgg 23

<210> 63

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 63

aggccgggag ctggaggagc tgg 23

<210> 64

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 64

agagcccccc ctcaaagaga ggg 23

<210> 65

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 65

ggagccacag gagccgctgc agg 23

<210> 66

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 66

tactcccagg tcctcttcaa ggg 23

<210> 67

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 67

ggcccagact gagcacgtga tgg 23

<210> 68

<211> 678

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 68

gaaaccggac cagtggcagt ggacccaacc ctgaggagac ggattgagcc ccatgaattt 60

gaagtgttct ttgacccaag ggagctgagg aaggagacat gcctgctgta cgagatcaag 120

tggggcacaa gccacaagat ctggcgccac agctccaaga acaccacaaa gcacgtggaa 180

gtgaatttca tcgagaagtt tacctccgag cggcacttct gcccctctac cagctgttcc 240

atcacatggt ttctgtcttg gagcccttgc ggcgagtgtt ccaaggccat caccgagttc 300

ctgtctcagc accctaacgt gaccctggtc atctacgtgg cccggctgta tcaccacatg 360

gaccagcaga acaggcaggg cctgcgcgat ctggtgaatt ctggcgtgac catccagatc 420

atgacagccc cagagtacga ctattgctgg cggaacttcg tgaattatcc acctggcaag 480

gaggcacact ggccaagata cccacccctg tggatgaagc tgtatgcact ggagctgcac 540

gcaggaatcc tgggcctgcc tccatgtctg aatatcctgc ggagaaagca gccccagctg 600

acatttttca ccattgctct gcagtcttgt cactatcagc ggctgcctcc tcatattctg 660

tgggctacag gcctgaag 678

<210> 69

<211> 609

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 69

atggaagcca gcccagcatc cgggcccaga cacttgatgg atccacacat attcacttcc 60

aactttaaca atggcattgg aaggcataag acctacctgt gctacgaagt ggagcgcctg 120

gacaatggca cctcggtcaa gatggaccag cacaggggct ttctacacaa ccaggctaag 180

aatcttctct gtggctttta cggccgccat gcggagctgc gcttcttgga cctggttcct 240

tctttgcagt tggacccggc ccagatctac agggtcactt ggttcatctc ctggagcccc 300

tgcttctcct ggggctgtgc cggggaagtg cgtgcgttcc ttcaggagaa cacacacgtg 360

agactgcgta tcttcgctgc ccgcatcttt gattacgacc ccctatataa ggaggcactg 420

caaatgctgc gggatgctgg ggcccaagtc tccatcatga cctacgatga atttaagcac 480

tgctgggaca cctttgtgga ccaccaggga tgtcccttcc agccctggga tggactagat 540

gagcacagcc aagccctgag tgggaggctg cgggccattc tccagaatca gggaaacagc 600

ggcagcgag 609

<210> 70

<211> 8855

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 70

atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 60

cccagtacat gaccttatgg gactttccta ctggcagtac atctacgtat tagtcatcgc 120

tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 180

acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 240

tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 300

gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 360

gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg aaacggacag 420

ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcagcagt gacaagaagt 480

acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt 540

acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga 600

agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga 660

agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga 720

tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct 780

tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg 840

aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca 900

gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc 960

ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt 1020

tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg 1080

gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc 1140

tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga 1200

gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc 1260

agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc 1320

agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca 1380

tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat 1440

acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg 1500

agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg 1560

gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg 1620

gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct 1680

tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc 1740

ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga 1800

ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga 1860

tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg 1920

gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg 1980

agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga 2040

ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga 2100

aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga 2160

aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag 2220

atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg 2280

acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac 2340

tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg 2400

acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga 2460

agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt 2520

ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta 2580

aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg 2640

ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg 2700

acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca 2760

gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg 2820

aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc 2880

agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg 2940

accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga 3000

gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg 3060

gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc 3120

agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga 3180

gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc 3240

ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg 3300

agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg 3360

atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc 3420

acgacgccta cctaaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg 3480

aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga 3540

gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact 3600

ttttcaagtc cggatccgag accccaggca cctccgagtc tgccacacct gagagcggaa 3660

gcatggaagc cagcccagca tccgggccca gacacttgat ggatccacac atattcactt 3720

ccaactttaa caatggcatt ggaaggcata agacctacct gtgctacgaa gtggagcgcc 3780

tggacaatgg cacctcggtc aagatggacc agcacagggg ctttctacac aaccaggcta 3840

agaatcttct ctgtggcttt tacggccgcc atgcggagct gcgcttcttg gacctggttc 3900

cttctttgca gttggacccg gcccagatct acagggtcac ttggttcatc tcctggagcc 3960

cctgcttctc ctggggctgt gccggggaag tgcgtgcgtt ccttcaggag aacacacacg 4020

tgagactgcg tatcttcgct gcccgcatct ttgattacga ccccctatat aaggaggcac 4080

tgcaaatgct gcgggatgct ggggcccaag tctccatcat gacctacgat gaatttaagc 4140

actgctggga cacctttgtg gaccaccagg gatgtccctt ccagccctgg gatggactag 4200

atgagcacag ccaagccctg agtgggaggc tgcgggccat tctccagaat cagggaaaca 4260

gcggcagcga gtctggatct ggcagcgaga caccaggaac aagcgagtca gcaacaccag 4320

agagcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca 4380

ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga 4440

caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca 4500

gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt 4560

ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag 4620

agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc 4680

tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact 4740

ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga 4800

agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact 4860

atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc 4920

acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc 4980

tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca 5040

tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg 5100

ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg 5160

tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc 5220

tgtctcagct gggaggtgac agcggcggga gcggcgggag cggggggagc actaatctga 5280

gcgacatcat tgagaaggag actgggaaac agctggtcat tcaggagtcc atcctgatgc 5340

tgcctgagga ggtggaggaa gtgatcggca acaagccaga gtctgacatc ctggtgcaca 5400

ccgcctacga cgagtccaca gatgagaatg tgatgctgct gacctctgac gcccccgagt 5460

ataagccttg ggccctggtc atccaggatt ctaacggcga gaataagatc aagatgctga 5520

gcggaggatc cggaggatct ggaggcagca ccaacctgtc tgacatcatc gagaaggaga 5580

caggcaagca gctggtcatc caggagagca tcctgatgct gcccgaagaa gtcgaagaag 5640

tgatcggaaa caagcctgag agcgatatcc tggtccatac cgcctacgac gagagtaccg 5700

acgaaaatgt gatgctgctg acatccgacg ccccagagta taagccctgg gctctggtca 5760

tccaggattc caacggagag aacaaaatca aaatgctgtc tggcggctca aaaagaaccg 5820

ccgacggcag cgaattcgag cccaagaaga agaggaaagt ctaaccggtc atcatcacca 5880

tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 5940

tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 6000

ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 6060

gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 6120

ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct cgataccgtc 6180

gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 6240

tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctaggatgc 6300

ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 6360

aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcgggaagag gcggtttgcg 6420

tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 6480

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 6540

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 6600

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 6660

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 6720

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 6780

cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 6840

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 6900

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 6960

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 7020

gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 7080

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 7140

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 7200

agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa actcacgtta 7260

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 7320

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 7380

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 7440

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 7500

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 7560

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 7620

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 7680

cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 7740

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 7800

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 7860

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 7920

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 7980

ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 8040

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 8100

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 8160

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 8220

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 8280

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 8340

atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct 8400

agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc 8460

tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca 8520

aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg 8580

cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc 8640

aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 8700

aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 8760

tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 8820

gtaaactgcc cacttggcag tacatcaagt gtatc 8855

<210> 71

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 71

ggtctctgat ccggcgcacg aa 22

<210> 72

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 72

ggtctctgat ccggcgcacg aa 22

<210> 73

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 73

gacaagaagt acagcatcgg cc 22

<210> 74

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 74

gctgtacttc ttgtcactgc tgactttccg cttcttc 37

<210> 75

<211> 36

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 75

gaagaagcgg aaagtcgaca agaagtacag catcgg 36

<210> 77

<211> 42

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 77

ctcactgatt aagcattggt aagcgcggaa cccctatttg tt 42

<210> 78

<211> 48

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 78

ccgtttcatg gtggcatgta tatctccttc ttaaagttaa acaaaatt 48

<210> 79

<211> 59

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 79

gtataatact agtgctcttg cccggcgtca atacgtttta gagctagaaa tagcaagtt 59

<210> 80

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 80

gttagcagcc ggatcaaaaa aagcaccgac tcgg 34

<210> 81

<211> 44

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 81

ttgacagcta gctcagtcct aggtataata ctagtgctct tgcc 44

<210> 82

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 82

gttagcagcc ggatcaaaaa aagcaccgac tcgg 34

<210> 83

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 83

cttttcgggg aaatgtggga aatgtgcgcg gaacc 35

<210> 84

<211> 20

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 84

cccggcgtca atacgggata 20

<210> 85

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 85

gtattgacgc cgggtaagag caactcggtc gccgc 35

<210> 86

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 86

ttaccaatgc ttaatcagtg aggcacc 27

<210> 87

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 87

cttttcgggg aaatgtggga aatgtgcgcg gaacc 35

<210> 88

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 88

cggatgccta gacaggtgtt caa 23

<210> 89

<211> 34

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 89

agggagagcc gccaccatga aacggacagc cgac 34

<210> 90

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 90

tcctcttctt cttgggctcg aattcgctgc cgtcggc 37

<210> 91

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 91

ggtggcggct ctccctatag tgagtc 26

<210> 92

<211> 26

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 92

cccaagaaga agaggaaagt ctaacc 26

<210> 93

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 93

catgaacttt ttcaagtccg gatccgagac cccaggc 37

<210> 94

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 94

tttcgccgtt tgtctcgctc tctggtgttg ctgac 35

<210> 94

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 94

tttcgccgtt tgtctcgctc tctggtgttg ctgac 35

<210> 95

<211> 37

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 95

catgaacttt ttcaagtccg gatccgagac cccaggc 37

<210> 96

<211> 35

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 96

tttcgccgtt tgtctcgctc tctggtgttg ctgac 35

<210> 97

<211> 27

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 97

gagacaaacg gcgaaaccgg ggagatc 27

<210> 98

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 98

cttgaaaaag ttcatgatgt tgc 23

<210> 99

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 99

atgcctgcta ttgtcttccc aa 22

<210> 100

<211> 21

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 100

aacgggactt tccaaaatgt c 21

<210> 101

<211> 24

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 101

tctcgcgcgt ttcggtgatg acgg 24

<210> 102

<211> 25

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 102

aaaaaaatct cgccaacaag ttgac 25

<210> 103

<211> 23

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 103

aaagatcttc acaggctacc ccc 23

<210> 104

<211> 22

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 104

aatccacagc aacaccctct cc 22

Claims

1.一种融合蛋白，所述融合蛋白自N端至C端依次包括第一nCas9片段、嵌合插入片段、第二nCas9片段和两个UGI片段，所述嵌合插入片段选自APOBEC1片段或APOBEC3A片段。

2.如权利要求1所述的融合蛋白，其特征在于，所述第一nCas9片段的氨基酸序列包括：

a)如SEQ ID NO.1所示的氨基酸序列；或，

和/或，所述第二nCas9片段的氨基酸序列包括：

c)如SEQ ID NO.2所示的氨基酸序列；或，

3.如权利要求1所述的融合蛋白，其特征在于，所述APOBEC1片段的氨基酸序列包括：

e)如SEQ ID NO.3所示的氨基酸序列；或，

4.如权利要求1所述的融合蛋白，其特征在于，所述APOBEC3A片段的氨基酸序列包括：

i)如SEQ ID NO.4所示的氨基酸序列；或，

5.如权利要求1所述的融合蛋白，其特征在于，所述UGI片段的氨基酸序列包括：

k)如SEQ ID NO.5所示的氨基酸序列；或，

6.如权利要求1所述的融合蛋白，其特征在于，所述融合蛋白还包括核定位信号片段，优选的，所述核定位信号片段的氨基酸序列包括SEQ ID NO.6所示的氨基酸序列。

7.如权利要求1所述的融合蛋白，其特征在于，所述融合蛋白还包括柔性连接肽片段，优选的，所述柔性连接肽片段的氨基酸序列包括SEQ ID NO.7或SEQ ID NO.8所示的氨基酸序列。

8.如权利要求1所述的融合蛋白，其特征在于，所述融合蛋白的氨基酸序列如SEQ IDNo.

9～10其中之一所示。

9.一种分离的多核苷酸，编码如权利要求1～8任一权利要求所述的融合蛋白。

10.一种构建体，所述构建体含有如权利要求9所述的分离的多核苷酸。

11.一种表达系统，所述表达系统含有如权利要求10所述的构建体或基因组中整合有外源的如权利要求9所述的多核苷酸。

12.如权利要求11所述的表达系统，其特征在于，所述表达系统的宿主细胞选自真核细胞或原核细胞，优选选自小鼠细胞、人细胞，更优选选自小鼠脑神经瘤细胞、人胚胎肾细胞、或人宫颈癌细胞、人结肠癌细胞、人骨肉瘤细胞，更优选选自N2a细胞、HEK293FT细胞、Hela细胞、HCT116细胞、或U2OS细胞。

13.如权利要求1～8任一权利要求所述的融合蛋白、如权利要求9所述的分离的多核苷酸、如权利要求10所述的构建体或如权利要求11～12任一权利要求所述的表达系统在基因编辑中的用途。

14.如权利要求13所述的用途，其特征在于，所述用途具体为在真核生物的基因编辑中的用途。

15.一种碱基编辑体系，包括如权利要求1～8任一权利要求所述的融合蛋白，所述碱基编辑体系还包括sgRNA。

16.一种基因编辑方法，包括：通过如权利要求1～8任一权利要求所述的融合蛋白、或如权利要求15所述的碱基编辑体系进行基因编辑。