CN111165758A - Anti-fatigue Baoleguo oral liquid and preparation method and application thereof - Google Patents

Anti-fatigue Baoleguo oral liquid and preparation method and application thereof Download PDF

Info

Publication number
CN111165758A
CN111165758A CN202010037125.7A CN202010037125A CN111165758A CN 111165758 A CN111165758 A CN 111165758A CN 202010037125 A CN202010037125 A CN 202010037125A CN 111165758 A CN111165758 A CN 111165758A
Authority
CN
China
Prior art keywords
fatigue
oral liquid
bordeaux
powder
tylophora
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010037125.7A
Other languages
Chinese (zh)
Inventor
焦红
潘滔
胡烨敏
龙山
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangzhou Boluohuo Biotechnology Co ltd
Original Assignee
Guangzhou Boluohuo Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Boluohuo Biotechnology Co ltd filed Critical Guangzhou Boluohuo Biotechnology Co ltd
Priority to CN202010037125.7A priority Critical patent/CN111165758A/en
Publication of CN111165758A publication Critical patent/CN111165758A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/01—Instant products; Powders; Flakes; Granules
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A23L29/37—Sugar alcohols
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/74—Rubiaceae (Madder family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Inorganic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Medical Informatics (AREA)
  • Epidemiology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention discloses an anti-fatigue Baoleguo oral liquid and a preparation method and application thereof. The anti-fatigue Baoleguo oral liquid comprises the following raw materials in percentage by weight: 10-30% of Bordeaux powder, 68-88% of water and the balance of flavoring agent. The preparation process provided by the invention is simple, the raw material proportion is scientific, the boletus oral liquid is rich in iridoid glycoside, triterpenes, sterols, polyphenol, total flavonoids, various organic acids, superoxide dismutase, various essential trace elements for human bodies and other nutrient components, and has the advantages of naturally supplementing nutrients for human bodies and being natural and free of additives; the Bordeaux oral liquid not only can reduce the content of serum urea and lactic acid after exercise, but also has the function of increasing the serum testosterone and the liver glycogen storage of male animals or reducing the glycogen consumption; during the exercise, the aerobic metabolism can be effectively increased, and the fatigue can be rapidly eliminated and the physical strength can be recovered, so that the adaptability of the human body to exercise load and the exercise capacity of the body can be improved, and the anti-fatigue effect can be achieved.

Description

Anti-fatigue Baoleguo oral liquid and preparation method and application thereof
Technical Field
The invention relates to the technical field of health care products, in particular to an anti-fatigue Baoleguo oral liquid and a preparation method and application thereof.
Background
Bordeaux belongs to the family of Rubiaceae and is a fruit that grows in Ecuador in south America. When the skin of the tylophora fruits turns brown from green and astringent and naturally falls to the ground from fruit trees, the tylophora fruits are ripe. The ripe fruit pulp is soft and rich in seeds, sour and thick in taste, and the pulp accounts for 88% after the seeds are removed. Bora fruits have a special aroma, are strong and long-lasting, and for thousands of years, the locally person Ecuador considers Bora fruits to be rich in nutrition and regards it as an extremely valuable food. The Borojo fruit is subjected to a series of procedures of cleaning, pulping, removing impurities such as seeds, peels and the like to form Borojo fruit pulp, added with 1% of pectinase for enzymolysis, and then subjected to concentration and spraying to obtain Borojo fruit powder. The research proves that the omethoate powder is rich in natural iridoid glycosides, polyphenol, flavonoid antioxidant substances, and rare natural nutrients such as B vitamins, mineral substances, essential amino acids of human bodies and the like in common tropical fruits.
The tylophora fruits are fruits with high nutritive value, and no report of the application of the tylophora fruits in fatigue resistance exists at present.
Disclosure of Invention
The invention aims to provide an anti-fatigue Baoleguo oral liquid and a preparation method and application thereof, so as to solve the technical problems.
In order to achieve the purpose, the technical scheme of the invention is as follows:
in a first aspect, the anti-fatigue tylophora oral liquid provided by the invention comprises the following raw materials in percentage by weight: 10-30% of Bordeaux powder, 68-88% of water and the balance of flavoring agent.
Preferably, the flavouring agent is selected from one or more of xylitol, maltodextrin, glucose and maltose.
Preferably, in the Bordeaux powder, the content of carbohydrate is 81.7-84.4%, the content of protein is 1.3-1.43%, the content of total acid is 1.4-1.42%, and the content of total sugar is 35.4-37.8%.
Preferably, the nutritional ingredients and the contents thereof in the Baole fruit powder are as follows: 1330-1496 mg/kg of phosphate, 15724.5-15956.44 mg/kg of potassium, 496.79-526.10 mg/kg of calcium, 983.13-985.17 mg/kg of magnesium, 10783.05-14195.64 mg/kg of sodium, 36.99mg/kg of iron and vitamin B22.10-2.47 mg/kg, vitamin B34.98-6.13 mg/kg of vitamin B537.1-44.8 mg/kg, 94.9g/100g of crude polysaccharide, 265.0-275.0 mg/100g of polyphenol, 1.90-1.94 g/100g of cycloolefine ether terpene glycoside, 2.10g/100g of triterpene and sterol, 152.6mg/100g of total flavone, 5.1mg/100g of lutein, 5.406g/100g of organic acid and 1050U/ml of superoxide dismutase.
In a second aspect, the invention provides a preparation method of the anti-fatigue tylophora oral liquid, which comprises the following steps:
mixing Bordeaux powder with water, stirring to completely dissolve Bordeaux powder in water, adding flavoring agent, stirring, sterilizing, and bottling to obtain antifatigue Bordeaux oral liquid.
In a third aspect, the invention provides an application of the anti-fatigue tylophora oral liquid in preparation of an anti-fatigue health food.
In a fourth aspect, the invention provides an application of the anti-fatigue tylophora oral liquid in preparation of an anti-fatigue medicine.
Compared with the prior art, the invention has the beneficial effects that:
the anti-fatigue tylophora oral liquid provided by the invention has the advantages of simple preparation process, scientific raw material proportion, harmonious taste, moderate sweetness, purity and no impurity, has typical tylophora flavor, is rich in nutritional ingredients such as Borojo-iridoid glycoside, triterpenes, sterols, polyphenol, total flavonoids, various organic acids, superoxide dismutase, various essential trace elements for human bodies, and the like, and has the advantages of naturally supplementing nutrients for human bodies and being natural and free of additives. The Bordeaux oral liquid not only can reduce serum urea after exercise and reduce the content of lactic acid, but also can increase the content of serum testosterone and liver glycogen of male animals and reduce the consumption of glycogen; in the process of exercise, aerobic metabolism can be effectively increased, fatigue can be rapidly eliminated, pain caused by lactic acid deposition can be relieved, and physical strength can be quickly recovered, so that the adaptability of a human body to exercise load and the exercise capacity of the body can be improved, and the effect of resisting fatigue can be achieved.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example 1
Weighing the following raw materials in percentage by weight: 20% of Bordeaux powder, 78% of drinking water, 1% of xylitol and 1% of maltodextrin; mixing Bordeaux powder with water, stirring to completely dissolve Bordeaux powder in water, adding flavoring agent, stirring, sterilizing, and bottling to obtain antifatigue Bordeaux oral liquid.
Example 2
Weighing the following raw materials in percentage by weight: 30% of Bordeaux powder, 68% of drinking water, 1% of xylitol and 1% of maltodextrin; mixing Bordeaux powder with water, stirring to completely dissolve Bordeaux powder in water, adding flavoring agent, stirring, sterilizing, and bottling to obtain antifatigue Bordeaux oral liquid.
Example 3
Weighing the following raw materials in percentage by weight: 25% of Baole fruit powder, 74% of drinking water, 0.5% of xylitol and 0.5% of maltodextrin; mixing Bordeaux powder with water, stirring to completely dissolve Bordeaux powder in water, adding flavoring agent, stirring, sterilizing, and bottling to obtain antifatigue Bordeaux oral liquid.
Example 4
Weighing the following raw materials in percentage by weight: 22% of Bordeaux powder, 76% of drinking water, 1% of xylitol and 1% of maltodextrin; mixing Bordeaux powder with water, stirring to completely dissolve Bordeaux powder in water, adding flavoring agent, stirring, sterilizing, and bottling to obtain antifatigue Bordeaux oral liquid.
Example 5
Weighing the following raw materials in percentage by weight: 15% of Bordeaux powder, 82% of drinking water, 1.5% of xylitol and 1.5% of maltodextrin; mixing Bordeaux powder with water, stirring to completely dissolve Bordeaux powder in water, adding flavoring agent, stirring, sterilizing, and bottling to obtain antifatigue Bordeaux oral liquid.
Example 6
Weighing the following raw materials in percentage by weight: 12% of Baole fruit powder, 80% of drinking water, 4% of xylitol and 4% of maltodextrin; mixing Bordeaux powder with water, stirring to completely dissolve Bordeaux powder in water, adding xylitol and maltodextrin, stirring, sterilizing, and bottling to obtain the antifatigue Bordeaux oral liquid.
Example 7
Weighing the following raw materials in percentage by weight: 10% of Baole fruit powder, 88% of drinking water, 1% of xylitol and 1% of maltodextrin; mixing Bordeaux powder with water, stirring to completely dissolve Bordeaux powder in water, adding xylitol and maltodextrin, stirring, sterilizing, and bottling to obtain the antifatigue Bordeaux oral liquid.
Effect example 1 measurement of serum testosterone
(1) Grouping: 80 male NIH mice with the age of 3-4 weeks and the weight of 18-22 g are selected and randomly divided into 8 groups, wherein seven groups take the oral liquid obtained in the examples 1-7 respectively, and the other group is given with the same volume of purified water, which is shown in the table 1.
(2) The administration method comprises the following steps: and (3) intragastric administration, wherein the liquid medicine is uniformly stirred all the time in the administration process, the administration is carried out for 6 days every week, and the administration is stopped for 1 day. The administration is carried out once respectively in the morning and afternoon of the administration period, during which the mice are fasted without water supply, and the administration amount is adjusted for 1 time per week according to the weight change; the administration time is 35 days;
(3) determination of serum testosterone: about 0.5mL of blood was drawn from the retroorbital venous plexus 60min after the last administration of the test sample to the mice (no anticoagulant added). Centrifuging at 2000pm/min for 15min after blood coagulation, and taking serum; the serum testosterone level was determined by a full-automatic immunoassay system using chemiluminescence, and the test results are shown in table 1.
TABLE 1 serum testosterone determination
Figure BDA0002366434480000031
Figure BDA0002366434480000041
Compared with a control group, the content of testosterone in serum of mice is remarkably improved and p is less than or equal to 0.05 after the oral liquid of the examples 1 to 7 is taken, wherein the effect is better when the oral liquid of the examples 1 to 5 is taken, and p is less than or equal to 0.01.
Effect example 2 measurement of serum Urea
(1) Grouping: 80 male NIH mice with the age of 3-4 weeks and the weight of 18-22 g are selected and randomly divided into 8 groups, wherein seven groups take the oral liquid obtained in the examples 1-7 respectively, and the other group is given with the same volume of purified water, which is shown in the table 1.
(2) The administration method comprises the following steps: and (3) intragastric administration, wherein the liquid medicine is uniformly stirred all the time in the administration process, the administration is carried out for 6 days every week, and the administration is stopped for 1 day. The administration is carried out once respectively in the morning and afternoon of the administration period, during which the mice are fasted without water supply, and the administration amount is adjusted for 1 time per week according to the weight change; the administration time is 35 days;
(3) after the mice are given the test sample for 60min in the last time, the mice swim in water with the temperature of 30 ℃ for 90min without load, and after resting for 60min, the retroorbital venous plexus is bled by about 0.5mL (without anticoagulant). Centrifuging at 2000pm/min for 15min after blood coagulation, and taking serum; the serum urea content was measured with a fully automatic biochemical analyzer and the results are shown in table 2.
TABLE 2 serum Urea measurement results
Figure BDA0002366434480000042
Compared with a control group, the serum urea content of the mice is obviously reduced by p ≦ 0.01 after the oral liquid of the examples 1-7 is taken.
Effect example 3 measurement of swimming time under load
(1) Grouping: 80 male NIH mice with the age of 3-4 weeks and the weight of 18-22 g are selected and randomly divided into 8 groups, wherein seven groups take the oral liquid obtained in the examples 1-7 respectively, and the other group is given with the same volume of purified water, which is shown in the table 1.
(2) The administration method comprises the following steps: and (3) intragastric administration, wherein the liquid medicine is uniformly stirred all the time in the administration process, and the administration is carried out for 6 days every week and is stopped for days. The administration is carried out once respectively in the morning and afternoon of the administration period, during which the mice are fasted without water supply, and the administration amount is adjusted for 1 time per week according to the weight change; the administration time is 35 days;
(3) after the test sample is given for 60min at the last time, a mouse with 5% weight lead skin loaded at the tail root is placed in a swimming box for swimming; the water depth is not less than 30cm, and the water temperature is 25 +/-1.0 ℃. The time from the start of swimming to death of the mice, i.e., the time for the mice to swim with a load, was observed, and the test results are shown in table 3.
TABLE 3 measurement results of swimming time under load
Figure BDA0002366434480000051
Compared with a control group, the swimming time of the mice is remarkably improved and p is less than or equal to 0.01 after the oral liquid of the examples 1 to 7 is taken, wherein the swimming time is better prolonged when the oral liquid of the examples 1 to 5 is taken, and p is less than or equal to 0.01.
Effect example 4 measurement of liver glycogen content
Grouping and dosing are carried out according to the steps (1) and (2) of the effect example 1, animals are killed after 60min of dosing, livers are taken, rinsed by normal saline and then sucked by filter paper, 1g of the livers are accurately weighed, the content of glycogen is measured by a glycogen liver kit, and the test results are shown in table 4.
TABLE 4 measurement results of hepatic glycogen
Figure BDA0002366434480000052
Figure BDA0002366434480000061
Compared with a control group, the hepatic glycogen content of the mice is remarkably improved by p less than or equal to 0.01 after the oral liquid of the examples 1 to 7 is taken, wherein the p more effective after the oral liquid of the examples 1 to 5 is taken is less than or equal to 0.01.
Effect example 5
In order to further illustrate the beneficial effects of the invention, the effects of different dosages of the oral liquid on mouse serum testosterone, serum urea, swimming time and liver glycogen are respectively tested according to the methods of effect examples 1-4, and the test results are shown in table 5.
TABLE 5 results of serum testosterone, serum urea and liver glycogen measurements
Figure BDA0002366434480000062
The test results show that the content of serum testosterone and liver glycogen of mice in each dose group is obviously higher than that of a control group, and the content of serum urea is obviously lower than that of the control group, which shows that the oral liquid of the tylosin has the effect of reducing the serum urea content of the mice after exercise, and the content of liver glycogen of the mice is increased along with the increase of the dose of the oral liquid, which shows that the oral liquid of the tylosin has the effect of obviously increasing the serum testosterone and glycogen storage of the mice or reducing glycogen consumption. The influence of the Bordeaux oral liquid on various indexes of a mouse is synthesized, and the Bordeaux oral liquid can effectively increase aerobic metabolism, quickly eliminate fatigue and recover physical strength, thereby improving the adaptability of the mouse to exercise load and the exercise capacity of a body.
EXAMPLE 6 assay of blood lactate and blood SOD levels in gymnasium volunteers
Preparing an anti-fatigue oral liquid containing 30 percent and 15 percent of Baole fruit powder according to the example 2 and the example 5, and filling the anti-fatigue oral liquid into a colored glass bottle with the net content of 200 ml; the control blind sample was filled with purified water in a 200ml glass bottle.
Selecting male young 15 people of a certain health club, wherein the average age is 24 plus or minus 6 years, and the average weight is 70 kg. Randomized into three groups of 5 people each. Before training, the first group is a control group, and the blind sample is taken with distilled water with the same volume; the second group takes the anti-fatigue oral liquid with the content of the tylophora fruit powder of 30 percent, and the third group takes the anti-fatigue oral liquid with the content of the tylophora fruit powder of 15 percent.
Three groups of volunteers used the same training subjects within 5 hours a day, venous blood was drawn at 3ml before and after training, and blood lactate and SOD were tested. The blood lactic acid kit and the SOD kit were used for the test. The apparatus used was an Yapeh Biochemical apparatus (model C80000). The test results are shown in table 5.
TABLE 5 volunteer blood lactate and SOD determination results
Figure BDA0002366434480000071
Compared with the control group, the blood lactic acid content of the volunteers is obviously reduced by p ≦ 0.01 after the group 1 and the group 2 take the oral liquid of the example 2 and the example 5; the SOD content is obviously improved by p ≦ 0.01; the control group has no significant difference in blood lactic acid before and after taking purified water, and p is not less than 0.05. After the group 1 and the group 2 take the oral liquid of example 2 and 5 respectively, the difference of the blood lactic acid content reduction in the blood of 2 groups of volunteers is more significant and p is less than or equal to 0.05; the difference of the increase of SOD content is also more significant, and p is less than or equal to 0.05; the control group has no significant difference in blood lactic acid before and after taking purified water, and p is not less than 0.05. The oral liquid of example 2 is better than the oral liquid of example 5.
Under the condition of anaerobic exercise in the gymnasium, muscles obtain energy through glycolysis reaction and simultaneously generate a large amount of lactic acid. The increase of lactic acid increases the concentration of H + in the muscle, and the decrease of ph is a significant cause of soreness of the tired muscle, and the more lactic acid is accumulated in the muscle, the more severe the fatigue degree becomes. The substance for reducing blood lactic acid content can play a role in reducing fatigue. The active substances rich in the tylophora fruits can reduce the content of lactic acid by increasing the content of lactic dehydrogenase so as to decompose and reduce the deposition of the lactic acid; in addition, the supplement of high-content potassium, sodium, calcium, magnesium and phosphorus ions also quickly supports the acid-base metabolic balance in the sports body, and comprehensively achieves the anti-fatigue effect.
Meanwhile, fatigue caused by anaerobic gymnasium exercise is related to rapid increase of free radical generation in vivo. The imbalance between oxygen free radicals and an antioxidant system can be caused during short-time strenuous exercise of a human body, so that a large amount of active oxygen is generated, extensive cell and tissue damage is caused, various pathological disorders are generated, and the working capacity of muscles is reduced to generate fatigue. The Bordeaux oral liquid contains high content of SOD, polyphenol, terpenoid, flavonoid and organic acid, and can supplement SOD activity and reduce MDA content in sports body before sports, thereby enhancing oxygen free radical scavenging ability of body and achieving anti-fatigue effect.
In order to further illustrate the beneficial effects of the invention, the nutritional ingredients contained in the omethoate powder adopted in the embodiment of the invention are tested, and the test results are as follows:
tests show that in the Baoleguo powder adopted in the embodiment of the invention, the content of carbohydrate is 81.7-84.4%, the content of protein is 1.3-1.43%, the content of total acid is 1.4-1.42%, the content of total sugar is 35.4-37.8%, and the calorie content is 351.2-343.9 calories.
The other nutritional ingredients and the contents of the Baole fruit powder adopted by the embodiment of the invention are as follows: 1330-1496 mg/kg of phosphate, 15724.5-15956.44 mg/kg of potassium, 496.79-526.10 mg/kg of calcium, 983.13-985.17 mg/kg of magnesium, 10783.05-14195.64 mg/kg of sodium, 36.99mg/kg of iron and vitamin B22.10～2.47mg/kg, vitamin B34.98-6.13 mg/kg of vitamin B537.1-44.8 mg/kg, 94.9g/100g of crude polysaccharide, 265.0-275.0 mg/100g of polyphenol, 1.90-1.94 g/100g of cycloolefine ether terpene glycoside, 2.10g/100g of triterpene and sterol, 152.6mg/100g of total flavone, 5.1mg/100g of lutein, 5.406g/100g of organic acid and 1050U/ml of superoxide dismutase.
The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.

Claims (7)

1. The anti-fatigue Baoleguo oral liquid is characterized by comprising the following raw materials in percentage by weight: 10-30% of Bordeaux powder, 68-88% of water and the balance of flavoring agent.
2. The anti-fatigue tylophora oral liquid of claim 1, wherein the flavoring agent is selected from one or more of xylitol, maltodextrin, glucose and maltose.
3. The anti-fatigue tylophora oral liquid according to claim 1, wherein the tylophora powder contains 81.7 to 84.4% of carbohydrate, 1.3 to 1.43% of protein, 1.4 to 1.42% of total acid and 35.4 to 37.8% of total sugar.
4. The anti-fatigue boletus oral liquid as claimed in claim 1, wherein the nutrient components and the content thereof in the boletus powder are as follows: 1330-1496 mg/kg of phosphate, 15724.5-15956.44 mg/kg of potassium, 496.79-526.10 mg/kg of calcium, 983.13-985.17 mg/kg of magnesium, 10783.05-14195.64 mg/kg of sodium, 36.99mg/kg of iron and vitamin B22.10-2.47 mg/kg, vitamin B34.98-6.13 mg/kg of vitamin B537.1-44.8 mg/kg, 94.9g/100g of crude polysaccharide, 265.0-275.0 mg/100g of polyphenol and cyclo-ring1.90-1.94 g/100g of rare ether terpene glycoside, 2.10g/100g of triterpene and sterol, 152.6mg/100g of total flavone, 5.1mg/100g of lutein, 5.406g/100g of organic acid and 1050U/ml of superoxide dismutase.
5. A preparation method of the anti-fatigue Baoleguo oral liquid as claimed in any one of claims 1 to 4, which comprises the following steps:
mixing Bordeaux powder with water, stirring to completely dissolve Bordeaux powder in water, adding flavoring agent, stirring, sterilizing, and bottling to obtain antifatigue Bordeaux oral liquid.
6. The application of the anti-fatigue tylophora oral liquid of any one of claims 1 to 4 in preparation of anti-fatigue health food.
7. The use of the anti-fatigue tylophora oral liquid according to any one of claims 1 to 4 in the preparation of an anti-fatigue medicament.
CN202010037125.7A 2020-01-14 2020-01-14 Anti-fatigue Baoleguo oral liquid and preparation method and application thereof Pending CN111165758A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010037125.7A CN111165758A (en) 2020-01-14 2020-01-14 Anti-fatigue Baoleguo oral liquid and preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010037125.7A CN111165758A (en) 2020-01-14 2020-01-14 Anti-fatigue Baoleguo oral liquid and preparation method and application thereof

Publications (1)

Publication Number Publication Date
CN111165758A true CN111165758A (en) 2020-05-19

Family

ID=70620609

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010037125.7A Pending CN111165758A (en) 2020-01-14 2020-01-14 Anti-fatigue Baoleguo oral liquid and preparation method and application thereof

Country Status (1)

Country Link
CN (1) CN111165758A (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1130068A (en) * 1995-06-16 1996-09-04 李丹 Oral liquor of snake essence
CN105747224A (en) * 2016-03-23 2016-07-13 焦红 Application of Borojoa sorbilis Cuatrec to assistance in blood glucose reduction
CN106174469A (en) * 2016-06-28 2016-12-07 烟台新时代健康产业有限公司 A kind of fatigue resistant health food compositions and health food preparation
CN108142805A (en) * 2018-01-23 2018-06-12 杭州艺福堂茶业有限公司 The compound meal replacement powder and its processing method of a kind of grain dust and precious Rogor powder
CN108497240A (en) * 2018-03-05 2018-09-07 天地壹号饮料股份有限公司 A kind of compound fruit vinegar beverage and preparation method thereof with auxiliary hyperglycemic function

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1130068A (en) * 1995-06-16 1996-09-04 李丹 Oral liquor of snake essence
CN105747224A (en) * 2016-03-23 2016-07-13 焦红 Application of Borojoa sorbilis Cuatrec to assistance in blood glucose reduction
CN106174469A (en) * 2016-06-28 2016-12-07 烟台新时代健康产业有限公司 A kind of fatigue resistant health food compositions and health food preparation
CN108142805A (en) * 2018-01-23 2018-06-12 杭州艺福堂茶业有限公司 The compound meal replacement powder and its processing method of a kind of grain dust and precious Rogor powder
CN108497240A (en) * 2018-03-05 2018-09-07 天地壹号饮料股份有限公司 A kind of compound fruit vinegar beverage and preparation method thereof with auxiliary hyperglycemic function

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
焦红: "植物源性新资源食品宝乐果(BOROJO)粉的营养和安全性研究", 《中国营养学会第十一次全国营养科学大会暨国际DRIS研讨会学术报告及论文摘要汇编（下册）——DRIS新进展：循证营养科学与实践学术》 *

Similar Documents

Publication Publication Date Title
CN103740566B (en) Rosa roxbunghii medicinal liquor and preparation method thereof
CN101669629B (en) Natural plant type functional health-care sports drink and manufacturing method thereof
CN101642176B (en) Method for preparing wild jujube honey tea for relieving neurasthenia
CN110192583A (en) With the composition and application for promoting intestinal health, control weight and raising to be immunized
CN115088835A (en) Multifunctional lycium ruthenicum enzyme stock solution and preparation process thereof
CN104388236B (en) A kind of grape wine and preparation method and application
CN101396115B (en) Xylo-oligosaccharide stomach-invigorating intestine-moisturizing oral liquid and preparation method thereof
CN101703296B (en) Pleurotus citrinopileatus drink and preparation method thereof
KR102062989B1 (en) Method for producing vinegar jelly for diet using healthful food powder
CN107446790A (en) A kind of fruit vinegar health-preserving beverage and preparation method thereof
CN113080344A (en) Collagen enzyme fruity beverage for promoting slimming and skin beautifying and preparation method thereof
KR20160059137A (en) MANUFACTURE OF FERMENTED Alliumhookeri FROM LACTIC ACID BACTERIA AND NATURAL ENZYME AND PREPARATION OF COMBINED BEVERAGE FOR QUENCHING THIRST
CN106999532A (en) The enhancing of Motor execution ability and physical enhancement composition containing composite extract
KR20010055274A (en) Health augmentation food for the anti fatigue
CN110279052A (en) A kind of pectase beverage and preparation method thereof improving immunity
KR20010103065A (en) Diet composition comprising dietary fiber
CN111165758A (en) Anti-fatigue Baoleguo oral liquid and preparation method and application thereof
KR101102871B1 (en) Sweet Jelly Of Beans Composition Containing Adventitious Root Of Ginseng For Osmotic Promotion And Manufacturing Method Thereof And The Sweet Jelly Of Beans
CN101884408A (en) Ginkgo leaf leaching liquor and preparation method thereof
CN101690533B (en) Manufacturing method of red data tea beverage
KR100278206B1 (en) Health supplement composition for growth
CN101336705B (en) Health food and its preparation method
CN107801879A (en) A kind of peptide fruit juice for strengthening memory peptide and being compounded with tangerine orange fruit juice and preparation method thereof
CN102823686A (en) Eight-treasure Chinese ixeridium herb tea and preparation method thereof
KR101930145B1 (en) Fasting supplement composition

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination