CN111109276A - Pesticide composition for bemisia tabaci - Google Patents

Pesticide composition for bemisia tabaci Download PDF

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Publication number
CN111109276A
CN111109276A CN201911301365.7A CN201911301365A CN111109276A CN 111109276 A CN111109276 A CN 111109276A CN 201911301365 A CN201911301365 A CN 201911301365A CN 111109276 A CN111109276 A CN 111109276A
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Prior art keywords
bemisia tabaci
chlorbenzuron
pesticide
acetamiprid
field
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Inventor
朱丽梅
陈新建
宰学明
曲哲
徐敏
张波
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Shenzhen Songfeng Agricultural Technology Development Co Ltd
Jinling Institute of Technology
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Shenzhen Songfeng Agricultural Technology Development Co Ltd
Jinling Institute of Technology
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Priority to CN201911301365.7A priority Critical patent/CN111109276A/en
Publication of CN111109276A publication Critical patent/CN111109276A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/34Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/22Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides

Abstract

The invention discloses an insecticide composition for bemisia tabaci, which is prepared by compounding a bemisia tabaci insecticide and chlorbenzuron; the bemisia tabaci insecticide is any one of nitenpyram, spinosad and acetamiprid. The bemisia tabaci insecticide and the chlorbenzuron are compounded to obtain the insecticide composition, and the adult and egg pests of the bemisia tabaci in the field can be killed simultaneously through the complementarity of the two insecticides, so that the control effect of the bemisia tabaci in the field can be obviously improved.

Description

Pesticide composition for bemisia tabaci
Technical Field
The invention relates to control of bemisia tabaci, in particular to an insecticide composition with a synergistic effect on the control of the bemisia tabaci.
Background
Bemisia tabaci (Gennadius) is a global pest seriously harming agricultural production, and because the Bemisia tabaci covers wax on the body surface, the control is difficult, the drug resistance is easy to generate, the propagation speed is high, and the generation overlapping is serious, the field control difficulty is high, the chemical control is still the main control measure of the Bemisia tabaci, and common medicaments comprise nitenpyram, spinosad, acetamiprid and the like.
The currently common bemisia tabaci control medicament has high insecticidal activity on adults, but has low or even ineffective control effect on eggs, the bemisia tabaci has high egg laying amount and high propagation speed, 3 forms of the bemisia tabaci exist in the same period, including eggs, nymphs and adults, and a plurality of farmers only kill the adults but ignore the eggs during control, so that the situation that the pesticide is applied for a few days later, the next bemisia tabaci gets up again, and the field control effect is poor.
Disclosure of Invention
The invention aims to solve the technical problem of the prior art and provides an insecticide composition with a synergistic effect on the control of bemisia tabaci so as to kill adult bemisia tabaci and eggs of bemisia tabaci simultaneously and solve the problems that the conventional bemisia tabaci insecticide has no ovicidal activity and the bemisia tabaci is rampant.
In order to achieve the purpose, the technical scheme adopted by the application is as follows:
an insecticide composition for bemisia tabaci is prepared by compounding bemisia tabaci insecticides and chlorbenzuron; the bemisia tabaci insecticide is any one of nitenpyram, spinosad and acetamiprid. The chlorbenzuron belongs to a chitin synthesis inhibitor of benzoyl urea insects, and is an insect hormone pesticide. The insecticidal composition is applied to larval stages, so that the formation of new epidermis of pests is blocked, the development is delayed, or the insects lack hardness, can not normally molt, and die or form abnormal pupas to die, and the insecticidal composition has good insecticidal activity on metamorphotic insects, particularly lepidoptera larvae. The pesticide can affect the respiratory metabolism of eggs and the DNA and protein metabolism in the process of embryo development, so that the larvae in the eggs lack chitin and can not hatch or die after hatching, thereby showing good ovicidal activity.
Specifically, the pesticide composition is prepared by compounding nitenpyram and chlorbenzuron, wherein the mass ratio of the nitenpyram to the chlorbenzuron is 1: 20-20: 1, and the preferable mass ratio is 1-10: 1. Nitenpyram belongs to the nicotinimide, and is another novel product developed after imidacloprid and acetamiprid in Japan. Has excellent systemic property, osmosis, broad insecticidal spectrum, safety and no phytotoxicity. Is a regeneration product for preventing and controlling pests with piercing-sucking mouthparts, such as whitefly, aphid, pear psylla, leafhopper and thrips.
Specifically, the pesticide composition is prepared by compounding spinosad and chlorbenzuron, wherein the mass ratio of the spinosad to the chlorbenzuron is 1: 20-20: 1, and the preferable mass ratio is 1-10: 1. Spinosad is a macrolide nuisance-free high-efficiency biopesticide extracted from a Saccharopolyspora spinosa fermentation broth, has quick contact poisoning and stomach poisoning effects on pests, has strong infiltration effect on leaves, can kill pests below the epidermis, has long residual effect period, and has a certain ovicidal effect on some pests. Has no systemic effect, and can effectively control lepidoptera, diptera and thysanoptera pests.
Specifically, the pesticide composition is prepared by compounding acetamiprid and chlorbenzuron, wherein the mass ratio of the acetamiprid to the chlorbenzuron is 1: 20-20: 1, and the preferable mass ratio is 1-10: 1. Acetamiprid belongs to a nicotine chloride compound, is a novel pesticide, acts on a nicotine acetylcholine receptor at a synapse part of an insect nervous system, interferes with stimulation conduction of the insect nervous system, causes block of a nervous system pathway, causes accumulation of neurotransmitter acetylcholine at the synapse part, and leads to paralysis and death of the insect.
The medicaments used in the method can be directly purchased from the market, and at present, a plurality of companies produce the medicaments on the market, and the medicaments have wide sources as long as the ratio range and the application condition of the invention are met.
Has the advantages that:
according to the invention, the bemisia tabaci insecticide and the chlorbenzuron are compounded to obtain the insecticide composition, and the adult and egg of the bemisia tabaci in the field can be simultaneously killed through the complementarity of the two insecticides, so that the control effect of the bemisia tabaci in the field can be obviously improved.
Detailed Description
The invention will be better understood from the following examples.
Example 1
1.1 location and time of implementation of the embodiment
The test was completed in the field of the Yan-Situ school district of Jinling science institute of Xixia, Nanjing city in 2019, and the greenhouse area was about 220m2About, the planting mode is 2 crops in 1 year.
1.2 crops of embodiment
The crop applied is eggplant.
1.3 Experimental methods
1.3.1 preparation of the medicament
Treatment 1 acetamiprid 100ppm + chlorbenzuron 100 ppm: the preparation method comprises the steps of mixing 1g of 20% acetamiprid water dispersant and 0.8ml of 25% chlorbenzuron suspending agent, and diluting to 2000 ml;
treatment 2 acetamiprid 100 ppm: the preparation method is that 20 percent acetamiprid water dispersant is diluted 2000 times;
treatment 3 acetamiprid 50ppm + chlorbenzuron 50 ppm: the preparation method comprises the steps of mixing 0.5g of 20% acetamiprid water dispersant and 0.4ml of 25% chlorbenzuron suspending agent, and diluting to 2000 ml;
treatment 4 acetamiprid 50 ppm: the preparation method is that 20 percent acetamiprid water dispersant is diluted 4000 times;
treatment 5 chlorbenzuron 100 ppm: the preparation method is 2500 times of 25 percent of chlorbenzuron suspending agent.
1.3.2 determination of field pesticide effect of the pesticide on eggplant Bemisia tabaci
The above drugs are respectively prepared and sprayed by a knapsack hand sprayer 3WBD-16, and the amount of the drug liquid is 50L (667 m)2) 1, study population number before drug, 3d, 5d and 10d after drugThe number of the surveys was 1 and 3 in total. 10 plants per cell at the mid-position, 9 a.m.: 00 previously the number of all Bemisia tabaci nymphs per plant was investigated. According to the population base before the pesticide and the population number of each day after the pesticide, the population reduction rate and the prevention and treatment effect of each treatment area and each control area are respectively calculated according to the following formulas, and the results are shown in table 1.
The reduction rate (%) of population (%) (pre-drug adult number-post-drug survival adult number)/pre-drug adult number × 100%.
The corrected oral cavity decline rate (%) (oral cavity decline rate in treatment area-oral cavity decline rate in blank control area)/(oral cavity decline rate in 100-blank control area) × 100%. The prevention and treatment effect is obtained by correcting the population reduction rate.
TABLE 1 field control Effect of Mixed Agenda and chlorbenzuron on Bemisia tabaci
Figure BDA0002321873800000031
As can be seen from Table 1, the mixed pesticide applied in the field has better control effect compared with the single acetamiprid preparation with the same concentration, wherein the corrected population reduction rate of 7d of the acetamiprid and the chlorbenzuron (100ppm and 100ppm) is 77.01%, the corrected population reduction rate of 10d is 90.91% and is higher than the field control effect of the single acetamiprid and the chlorbenzuron (50ppm and 50ppm), the corrected population reduction rate of 7d and 10d of the acetamiprid and the chlorbenzuron (50ppm and 50ppm) is 66.7% and 83.3% and is higher than the field control effect of the single acetamiprid and the single chlorbenzuron (10 ppm and 50ppm), the corrected population reduction rate of the single chlorbenzuron in the field is less than 5%, and the field efficacy of the acetamiprid can be obviously improved by adding the chlorbenzuron.
Example 2
2.1 location and time of implementation of the embodiment
The test was completed in a greenhouse of Cisco prefecture of Jinling science institute of Xixia, Nanjing city in 2019 and the area of the greenhouse was about 100m2About, the planting mode is 2 crops in 1 year.
2.2 crops of embodiment
The crop implemented is tomato.
2.3 Experimental methods
2.3.1 preparation of the medicament
Agent 1: adding 40% chlorbenzuron suspending agent into 80% nitenpyram water dispersible granules according to the ratio of 1:1 and diluting to 2500 times;
medicament 2: diluting 80% of nitenpyram water dispersible granules to 2500 times;
agent 3: the 40% chlorbenzuron suspension is diluted to 2500 times.
2.3.2 determination of field pesticide effect of the pesticide on eggplant Bemisia tabaci
The above drugs are respectively prepared and sprayed by a knapsack hand sprayer 3WBD-16, and the amount of the drug liquid is 50L (667 m)2) The population number of the insect before the drug is investigated, and 3 times of investigation are carried out after the drug in 3d, 5d and 10d respectively, and 3 times of investigation are carried out. 10 plants per cell at the mid-position, 9 a.m.: 00 previously the number of all Bemisia tabaci nymphs per plant was investigated. According to the population base before the pesticide and the number of the survival imagoes after the pesticide, the population reduction rate and the prevention and treatment effect of each treatment area and each control area are respectively calculated according to the following formulas. (calculation formula 1.3.2 in example 1)
TABLE 2 insecticidal and chlorbenzuron chemical combinations on Bemisia tabaci control
Figure BDA0002321873800000051
As can be seen from Table 2, the mixed pesticide applied in the field has better control effect on Bemisia tabaci, wherein the rate of entrance reduction of the nitenpyram and the chlorbenzuron 10d is 81.1 percent, which is higher than the field pesticide effect of a single pesticide 55.6 percent.
Example 3
3.1 location and time of implementation of the embodiment
The test was completed in a greenhouse of Cisco prefecture of Jinling science institute of Xixia, Nanjing, 11 months in 2019, the greenhouse area was about 100m2About, the planting mode is 2 crops in 1 year.
3.2 crops of embodiment
The crop implemented is tomato.
3.3 Experimental methods
3.3.1 preparation of the medicament
Agent 1: adding 40% chlorbenzuron suspending agent into 6% pleocidin according to the ratio of 1:1, and diluting to 2000 times;
medicament 2: 6% pleocidin is diluted to 2000 times;
agent 3: the 40% chlorbenzuron suspension is diluted to 2000 times.
3.3.2 determination of field pesticide effect of the pesticide on eggplant Bemisia tabaci
The above drugs are respectively prepared and sprayed by a knapsack hand sprayer 3WBD-16, and the amount of the drug liquid is 50L (667 m)2) The population number of the insect before the drug is investigated, and 3 times of investigation are carried out after the drug in 3d, 5d and 10d respectively, and 3 times of investigation are carried out. 10 plants per cell at the mid-position, 9 a.m.: 00 previously the number of all Bemisia tabaci nymphs per plant was investigated. According to the population base before the pesticide and the number of the survival imagoes after the pesticide, the population reduction rate and the prevention and treatment effect of each treatment area and each control area are respectively calculated according to the following formulas. (calculation formula 1.3.2 in example 1)
TABLE 3 control of Bemisia tabaci by combination of spinosad and chlorbenzuron agents
Figure BDA0002321873800000061
As can be seen from Table 3, the mixed application of the spinosad and the chlorbenzuron medicament in the field has better control effect on the bemisia tabaci, wherein the population reduction rate of the spinosad and the chlorbenzuron is 84.8 percent and is higher than the field effect of the single medicament of 51.6 percent.
Example 4
4.1 location and time of implementation of embodiment
The test is completed in the field of the Yanjing Cuifu school district of Jinling science institute of Xixia, Nanjing city in 2019 from 9 months to 2019 from 12 months, and the greenhouse area is about 220m2About, the planting mode is 2 crops in 1 year.
4.2 crops of embodiment
The crop applied is eggplant.
4.3 specific measures to be taken in embodiment
4.3.1 the chemical pesticide (20% acetamiprid water dispersible granule + 25% chlorbenzuron mixed at a ratio of 1: 0.8) was applied once at 20% acetamiprid water dispersible granule in 30 days at 9 months and once at 2000 times and at 19 days at 10 months, respectively, and then diluted 2000 times according to the amount of acetamiprid.
4.4 prevention and control results of this embodiment
From table 4, when the pest amount in 28 days at 9 months occurs a peak, the pesticide application amount of 40% acetamiprid water dispersible granule in the field is 2000 times, the pest population of the plant in the field at 7d is reduced to 153.3, the reduction rate is 44.8%, the pest population of the plant in the field at 14d is reduced to 148.8, the reduction rate is 46.4%, the pest amount of bemisia tabaci gradually increases to 207.8 in 19 days at 10 months, the pesticide effect of the acetamiprid alone can only be maintained at 14d, the pest population of the plant at 19 days at 10 months is increased to 207.8, the pesticide application amount of 40% acetamiprid water dispersible granule 2000 times and the ovicide application amount of the acetamiprid water dispersible granule in the field at the second time at 10 months are respectively 22.8% and 45.6%, and the pest population reduction rates of the plant at 9 days at 11 months and 16 days are respectively 58.7% and 94.5%, which shows that the acetamiprid + the ovicide application amount can achieve a relatively good control effect and can have a relatively long control effect in the field.
TABLE 4 Meshed eggplant Bemisia tabaci occurrence dynamics
Date of treatment Number of plant insect Reduction rate of insect population%
9.7 1.3 ——
9.14 0.0 ——
9.21 137.0 ——
9.30 (first)Second application of medicine 278.0 ——
10.7 (post-drug 7d) 153.3 44.8
10.14 (post-drug 14d) 148.8 46.4
10.19 (second application) 207.8 ——
10.26 (post-drug 7d) 160.5 22.8
11.02 (medicine 14d) 113.0 45.6
11.09 (post-drug 21d) 85.8 58.7
11.16 (post-drug 28d) 11.5 94.5
The present invention provides a method and a concept for a pesticide composition against bemisia tabaci, and a method and a way for implementing the technical scheme are many, and the above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should be regarded as the protection scope of the present invention. All the components not specified in the present embodiment can be realized by the prior art.

Claims (5)

1. The pesticide composition for bemisia tabaci is characterized by being prepared by compounding bemisia tabaci pesticides and chlorbenzuron.
2. The pesticide composition as claimed in claim 1, which is prepared by compounding nitenpyram and chlorbenzuron, wherein the mass ratio of the nitenpyram to the chlorbenzuron is 1: 20-20: 1.
3. The pesticide composition as claimed in claim 1, which is prepared by compounding spinosad and chlorbenzuron, wherein the mass ratio of the spinosad to the chlorbenzuron is 1: 20-20: 1.
4. The pesticide composition as claimed in claim 1, which is prepared by compounding acetamiprid and chlorbenzuron, wherein the mass ratio of the acetamiprid to the chlorbenzuron is 1: 20-20: 1.
5. The pesticide composition as claimed in any one of claims 2 to 4, wherein the bemisia tabaci pesticide and the chlorbenzuron are compounded in a mass ratio of 1-10: 1.
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