CN111109083A - Culture medium suitable for cultivating multiple sweet potato seedlings and preparation method thereof - Google Patents
Culture medium suitable for cultivating multiple sweet potato seedlings and preparation method thereof Download PDFInfo
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- CN111109083A CN111109083A CN202010044578.2A CN202010044578A CN111109083A CN 111109083 A CN111109083 A CN 111109083A CN 202010044578 A CN202010044578 A CN 202010044578A CN 111109083 A CN111109083 A CN 111109083A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses a culture medium suitable for cultivating various sweet potato seedlings and a preparation method thereof, wherein the culture medium comprises macroelements, microelements, organic non-inositol, ferric salt, disodium ethylene diamine tetraacetate, sucrose, NAA and agar, the macroelements comprise ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate, calcium chloride dihydrate and potassium dihydrogen phosphate, the microelements comprise potassium iodide, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate and cobalt chloride hexahydrate, and the organic non-inositol comprises nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride and glycine; the formula has the advantages that the proportion of each component is reasonable, the design of the preparation method is simple, the linkage is reasonable, the quality of sweet potato seedlings is obviously improved on the whole, the plants are thick and strong, the root system is developed, and the height of the plants in 15 days is 1.5-3 cm higher than that of the plants without the formula; the disease is reduced; the culture period can be shortened by 8-15 days.
Description
Technical Field
The invention belongs to the technical field of agricultural soilless culture, relates to a culture medium for sweet potato seedlings, and particularly relates to a culture medium suitable for cultivating various sweet potato seedlings and a preparation method thereof.
Background
Plant tissue culture is a new technology of asexual propagation developed based on the theory that plant cells have totipotency, and means to separate tissues, organs or cells and the like which meet the needs from plants. Inoculating the culture medium containing various nutrients and phytohormones under aseptic conditions.
The growth of the sweet potato plantlets mainly depends on macroelements, microelements, organic matters without inositol, iron salt, sucrose and hormone in a culture medium, and the demand of the sweet potato plantlets on various elements is different, so that the contents of various elements, sugar and hormone in the prepared culture medium are different.
Disclosure of Invention
The invention aims to provide a culture medium suitable for cultivating various sweet potato seedlings and a preparation method thereof, the culture medium can ensure that various elements required by a sweet potato plantlet in the culture process are fully met, the fast growth and development of the sweet potato plantlet are realized, and the preparation method is simple in design, reasonable in connection and extremely high in use value.
The purpose of the invention is realized as follows: a culture medium suitable for breeding seedlings of various sweet potatoes and a preparation method thereof, wherein the culture medium comprises macroelements, trace elements, organic non-inositol, ferric salt, ethylene diamine tetraacetic acid disodium, sucrose, NAA and agar, the macroelements comprise ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate, calcium chloride dihydrate and potassium dihydrogen phosphate, the trace elements comprise potassium iodide, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate and cobalt chloride hexahydrate, and the organic non-inositol comprises nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride and glycine;
the culture medium contains the following components in mass per liter:
14-16 g of ammonium nitrate, 16-18 g of potassium nitrate, 2.5-3.5 g of magnesium sulfate heptahydrate, 3.1-4.8 g of calcium chloride dihydrate, 0.9-1.8 g of monopotassium phosphate, 0.164-0.168 g of potassium iodide, 1.20-1.26 g of boric acid, 4.42-4.47 g of manganese sulfate tetrahydrate, 1.70-1.76 g of zinc sulfate heptahydrate, 0.04-0.06 g of sodium molybdate dihydrate, 0.004-0.006 g of copper sulfate pentahydrate, 0.004-0.006 g of cobalt chloride hexahydrate, 0.05-0.15 g of nicotinic acid, 0.05-0.15 g of pyridoxine hydrochloride, 0.01-0.03 g of thiamine hydrochloride, 0.3-0.5 g of glycine, 9-11 g of inositol, 2.75-2.80 g of ferrous sulfate heptahydrate, 3.70-3.75 g of disodium ethylenediaminetetraacetate, 25-35 g of sucrose, 0.01-0.03 g of NAA and 3.6 g of agar;
the preparation method of the culture medium comprises the following steps:
a. selecting a quantitative culture medium preparation vessel;
b. according to the volume of a preparation vessel, respectively weighing ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate, calcium chloride dihydrate, potassium dihydrogen phosphate, potassium iodide, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate, cobalt chloride hexahydrate, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride, glycine, inositol, ferrous sulfate heptahydrate, disodium ethylenediaminetetraacetate and NAA according to the formula proportion, and sequentially putting the raw materials;
c. weighing agar and sucrose with corresponding mass according to the volume of a preparation vessel, adding distilled water to the volume of 1/2-3/4 of the vessel, and heating to dissolve the agar;
d. sequentially adding ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate, calcium chloride dihydrate, potassium dihydrogen phosphate, potassium iodide, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate, cobalt chloride hexahydrate, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride, glycine, inositol, ferrous sulfate heptahydrate, disodium ethylenediamine tetraacetate and NAA into the solution obtained in the step c, stirring, and then cooling to room temperature;
e. adding distilled water into a preparation vessel to fix the volume to the final volume of the culture medium;
f. subpackaging the culture medium with constant volume into 350ml culture bottles, and covering a bottle cap or sealing a sealing film;
g. the culture bottle was placed in a sterilizer and sterilized.
Each liter of culture medium contains the following components by mass: 15g of ammonium nitrate, 17g of potassium nitrate, 3.0g of magnesium sulfate heptahydrate, 4.0g of calcium chloride dihydrate, 1.5g of potassium dihydrogen phosphate, 0.166g of potassium iodide, 1.23g of boric acid, 4.45g of manganese sulfate tetrahydrate, 1.73g of zinc sulfate heptahydrate, 0.05g of sodium molybdate dihydrate, 0.005g of copper sulfate pentahydrate, 0.005g of cobalt chloride hexahydrate, 0.10g of nicotinic acid, 0.12g of pyridoxine hydrochloride, 0.02g of thiamine hydrochloride, 0.4g of glycine, 10g of inositol, 2.79g of ferrous sulfate heptahydrate, 3.71g of disodium ethylenediaminetetraacetate, 28g of sucrose, 0.02g of NAA and 5g of agar.
The preparation vessel is a 100L electric heating stirring tank.
And d, setting the stirring time in the step d to be 5-8 minutes.
And g, sterilizing at 121 ℃ for 20 minutes in the step g.
And e, adjusting the pH value after the volume is fixed, wherein the pH value is 5.5-5.9, and dilute nitric acid with the mass fraction of 4-6% is adopted for adjusting the pH value.
The formula of the culture medium for cultivating the sweet potato seedlings is theoretically prepared based on analysis of soil nutrients in the natural growth environment of the sweet potato seedlings and the growth mechanism of the sweet potato seedlings, but the theoretical nutritional formula can only meet the basic requirements of plant growth and hardly meets the requirements of human commercial production. In order to achieve the purposes of good growth, high yield, high efficiency and the like, the most appropriate formula must be summarized in countless test comparisons through long-term production practice and tests.
The formula of the culture medium of the invention is an optimal scheme screened in comparison experiments of nearly thousands of schemes after production experiments for nearly four years, the components of the formula are reasonable in proportion, the pH value of the culture medium is proper, the sweet potato seedlings can grow and develop rapidly, and the culture medium is an optimal value required by the growth of the sweet potato seedlings.
Compared with the common technology, the invention has the following beneficial effects: the formula has reasonable proportion of each component, can ensure that various elements required by sweet potato plants in the culture process are fully satisfied, and realizes the rapid growth and development of the sweet potato plantlets. And can fully meet the requirement of photosynthesis of sweet potato plants and promote the growth and development of the sweet potato plants. The quality of sweet potato seedlings is obviously improved on the whole, the plants are thick and strong, the root system is developed, and the height of the plants in 15 days is generally 1.5-3 cm higher than that of the plants without the formula; the disease is reduced; the culture period can be shortened by 7-10 days.
Detailed Description
The present invention will be further described with reference to the following specific examples.
Example 1
A culture medium suitable for breeding seedlings of various sweet potatoes and a preparation method thereof, wherein the culture medium comprises macroelements, trace elements, organic non-inositol, ferric salt, ethylene diamine tetraacetic acid disodium, sucrose, NAA and agar, the macroelements comprise ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate, calcium chloride dihydrate and potassium dihydrogen phosphate, the trace elements comprise potassium iodide, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate and cobalt chloride hexahydrate, and the organic non-inositol comprises nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride and glycine;
the culture medium contains the following components in mass per liter:
14g of ammonium nitrate, 16g of potassium nitrate, 2.5g of magnesium sulfate heptahydrate, 3.1g of calcium chloride dihydrate, 0.9g of potassium dihydrogen phosphate, 0.164g of potassium iodide, 1.20g of boric acid, 4.42g of manganese sulfate tetrahydrate, 1.70g of zinc sulfate heptahydrate, 0.04g of sodium molybdate dihydrate, 0.004g of copper sulfate pentahydrate, 0.004g of cobalt chloride hexahydrate, 0.05g of nicotinic acid, 0.05g of pyridoxine hydrochloride, 0.01g of thiamine hydrochloride, 0.3g of glycine, 9g of inositol, 2.75g of ferrous sulfate heptahydrate, 3.70g of disodium ethylenediaminetetraacetate, 25g of sucrose, 0.01g of NAA and 3g of agar;
the preparation method of the culture medium comprises the following steps:
a. selecting a 100L electric heating stirring tank as a preparation vessel of the culture medium;
b. according to the volume of a preparation vessel, respectively weighing 1.4 g of ammonium nitrate, 1.6 g of potassium nitrate, 0.25 g of magnesium sulfate heptahydrate, 0.31 g of calcium chloride dihydrate, 0.09 g of potassium dihydrogen phosphate, 16.4g of potassium iodide, 120g of boric acid, 442g of manganese sulfate tetrahydrate, 170g of zinc sulfate heptahydrate, 4g of sodium molybdate dihydrate, 0.4g of copper sulfate pentahydrate, 0.4g of cobalt chloride hexahydrate, 5g of nicotinic acid, 5g of pyridoxine hydrochloride, 1g of thiamine hydrochloride, 30g of glycine, 900g of inositol, 275g of ferrous sulfate heptahydrate, 370g of disodium edetate and 1g of NAA according to the formula proportion, and sequentially putting the raw materials in the above order;
c. weighing 300g of agar and 2.5 kg of sucrose according to the volume of a preparation vessel, adding distilled water to 1/2 of the volume of the vessel, and heating to 95 ℃ to dissolve the agar;
d. sequentially adding ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate, calcium chloride dihydrate, potassium dihydrogen phosphate, potassium iodide, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate, cobalt chloride hexahydrate, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride, glycine, inositol, ferrous sulfate heptahydrate, disodium ethylenediamine tetraacetate and NAA into the solution obtained in the step c, stirring for 8 minutes, and then cooling to room temperature;
e. adding distilled water into a preparation vessel to fix the volume to the final volume of the culture medium, and adjusting the pH value to be between 5.5 and 5.9 by using dilute nitric acid with the mass fraction of 5 percent;
f. subpackaging the culture medium with constant volume into 350ml culture bottles, and covering a bottle cap or sealing a sealing film;
g. the culture bottle is placed in a sterilization pot for sterilization, the sterilization temperature is 121 ℃, and the sterilization time is 20 minutes.
Example 2
A culture medium suitable for breeding seedlings of various sweet potatoes and a preparation method thereof, wherein the culture medium comprises macroelements, trace elements, organic non-inositol, ferric salt, ethylene diamine tetraacetic acid disodium, sucrose, NAA and agar, the macroelements comprise ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate, calcium chloride dihydrate and potassium dihydrogen phosphate, the trace elements comprise potassium iodide, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate and cobalt chloride hexahydrate, and the organic non-inositol comprises nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride and glycine;
the culture medium contains the following components in mass per liter:
15g of ammonium nitrate, 17g of potassium nitrate, 3.0g of magnesium sulfate heptahydrate, 4.0g of calcium chloride dihydrate, 1.5g of potassium dihydrogen phosphate, 0.166g of potassium iodide, 1.23g of boric acid, 4.45g of manganese sulfate tetrahydrate, 1.73g of zinc sulfate heptahydrate, 0.05g of sodium molybdate dihydrate, 0.005g of copper sulfate pentahydrate, 0.005g of cobalt chloride hexahydrate, 0.10g of nicotinic acid, 0.12g of pyridoxine hydrochloride, 0.02g of thiamine hydrochloride, 0.4g of glycine, 10g of inositol, 2.79g of ferrous sulfate heptahydrate, 3.71g of disodium ethylenediaminetetraacetate, 28g of sucrose, 0.02g of NAA and 5g of agar;
the preparation method of the culture medium is the same as that of the example 1.
Example 3
A culture medium suitable for breeding seedlings of various sweet potatoes and a preparation method thereof, wherein the culture medium comprises macroelements, trace elements, organic non-inositol, ferric salt, ethylene diamine tetraacetic acid disodium, sucrose, NAA and agar, the macroelements comprise ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate, calcium chloride dihydrate and potassium dihydrogen phosphate, the trace elements comprise potassium iodide, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate and cobalt chloride hexahydrate, and the organic non-inositol comprises nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride and glycine;
the culture medium contains the following components in mass per liter:
16g of ammonium nitrate, 18g of potassium nitrate, 3.5g of magnesium sulfate heptahydrate, 4.8g of calcium chloride dihydrate, 1.8g of monopotassium phosphate, 0.168g of potassium iodide, 1.26g of boric acid, 4.47g of manganese sulfate tetrahydrate, 1.76g of zinc sulfate heptahydrate, 0.06g of sodium molybdate dihydrate, 0.006g of copper sulfate pentahydrate, 0.006g of cobalt chloride hexahydrate, 0.15g of nicotinic acid, 0.15g of pyridoxine hydrochloride, 0.03g of thiamine hydrochloride, 0.5g of glycine, 11g of inositol, 2.80g of ferrous sulfate heptahydrate, 3.75g of disodium ethylenediaminetetraacetate, 35g of sucrose, 0.03g of NAA and 6g of agar;
the preparation method of the culture medium is the same as that of the example 1.
Claims (7)
1. A culture medium suitable for cultivating multi-variety sweet potato seedlings and a preparation method thereof are characterized in that: the culture medium comprises macroelements, trace elements, organic non-inositol, ferric salt, ethylene diamine tetraacetic acid disodium, sucrose, NAA and agar, wherein the macroelements comprise ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate, calcium chloride dihydrate and potassium dihydrogen phosphate, the trace elements comprise potassium iodide, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate and cobalt chloride hexahydrate, and the organic non-inositol comprises nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride and glycine;
the culture medium contains the following components in mass per liter:
14-16 g of ammonium nitrate, 16-18 g of potassium nitrate, 2.5-3.5 g of magnesium sulfate heptahydrate, 3.1-4.8 g of calcium chloride dihydrate, 0.9-1.8 g of monopotassium phosphate, 0.164-0.168 g of potassium iodide, 1.20-1.26 g of boric acid, 4.42-4.47 g of manganese sulfate tetrahydrate, 1.70-1.76 g of zinc sulfate heptahydrate, 0.04-0.06 g of sodium molybdate dihydrate, 0.004-0.006 g of copper sulfate pentahydrate, 0.004-0.006 g of cobalt chloride hexahydrate, 0.05-0.15 g of nicotinic acid, 0.05-0.15 g of pyridoxine hydrochloride, 0.01-0.03 g of thiamine hydrochloride, 0.3-0.5 g of glycine, 9-11 g of inositol, 2.75-2.80 g of ferrous sulfate heptahydrate, 3.70-3.75 g of disodium ethylenediaminetetraacetate, 25-35 g of sucrose, 0.01-0.03 g of NAA and 3.6 g of agar;
the preparation method of the culture medium comprises the following steps:
a. preparing a quantitative culture medium into a vessel;
b. according to the volume of a preparation vessel, respectively weighing ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate, calcium chloride dihydrate, potassium dihydrogen phosphate, potassium iodide, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate, cobalt chloride hexahydrate, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride, glycine, inositol, ferrous sulfate heptahydrate, disodium ethylenediaminetetraacetate and NAA according to the formula proportion, and sequentially putting the raw materials;
c. weighing agar and sucrose with corresponding mass according to the volume of a preparation vessel, adding distilled water to the volume of 1/2-3/4 of the vessel, and heating to dissolve the agar;
d. sequentially adding ammonium nitrate, potassium nitrate, magnesium sulfate heptahydrate, calcium chloride dihydrate, potassium dihydrogen phosphate, potassium iodide, boric acid, manganese sulfate tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate, cobalt chloride hexahydrate, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride, glycine, inositol, ferrous sulfate heptahydrate, disodium ethylenediamine tetraacetate and NAA into the solution obtained in the step c, stirring, and then cooling to room temperature;
e. adding distilled water into a preparation vessel to fix the volume to the final volume of the culture medium;
f. subpackaging the culture medium with constant volume into 350ml culture bottles, and covering a bottle cap or sealing a sealing film;
g. the culture bottle was placed in a sterilizer and sterilized.
2. The culture medium suitable for breeding seedlings of multiple sweet potatoes and the preparation method thereof as claimed in claim 1, wherein the culture medium comprises: each liter of culture medium contains the following components by mass: 15g of ammonium nitrate, 17g of potassium nitrate, 3.0g of magnesium sulfate heptahydrate, 4.0g of calcium chloride dihydrate, 1.5g of potassium dihydrogen phosphate, 0.166g of potassium iodide, 1.23g of boric acid, 4.45g of manganese sulfate tetrahydrate, 1.73g of zinc sulfate heptahydrate, 0.05g of sodium molybdate dihydrate, 0.005g of copper sulfate pentahydrate, 0.005g of cobalt chloride hexahydrate, 0.10g of nicotinic acid, 0.12g of pyridoxine hydrochloride, 0.02g of thiamine hydrochloride, 0.4g of glycine, 10g of inositol, 2.79g of ferrous sulfate heptahydrate, 3.71g of disodium ethylenediaminetetraacetate, 28g of sucrose, 0.02g of NAA and 5g of agar.
3. The culture medium suitable for breeding seedlings of multiple sweet potatoes and the preparation method thereof as claimed in claim 1, wherein the culture medium comprises: the preparation vessel is a 100L electric heating stirring tank.
4. The culture medium suitable for breeding seedlings of multiple sweet potatoes and the preparation method thereof as claimed in claim 1, wherein the culture medium comprises: and d, setting the stirring time in the step d to be 5-8 minutes.
5. The culture medium suitable for breeding seedlings of multiple sweet potatoes and the preparation method thereof as claimed in claim 1, wherein the culture medium comprises: and g, sterilizing at 121 ℃ for 20 minutes in the step g.
6. The culture medium suitable for breeding seedlings of multiple sweet potatoes and the preparation method thereof as claimed in claim 1, wherein the culture medium comprises: and e, adjusting the pH value after the volume is fixed in the step e, wherein the pH value is 5.5-5.9.
7. The culture medium suitable for breeding seedlings of multiple sweet potatoes and the preparation method thereof as claimed in claim 1 or 6, wherein the culture medium comprises: and dilute nitric acid with the mass fraction of 4-6% is adopted for adjusting the pH value.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115843686A (en) * | 2022-12-03 | 2023-03-28 | 瑞盈优品(广东)农业科技有限责任公司 | Culture medium for sweet potato tissue culture and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674966A (en) * | 2012-05-21 | 2012-09-19 | 新疆生产建设兵团农六师农业科学研究所 | Special culture medium for potato virus-free seedling transplanting and preparation method thereof |
| CN103931500A (en) * | 2013-04-19 | 2014-07-23 | 云南农业大学 | Culture medium for promoting quick growth of potato tissue culture seedling |
| CN107148911A (en) * | 2017-03-29 | 2017-09-12 | 天津丰华裕隆农业发展有限公司 | A kind of sweet potato stem tip detoxification solid medium and preparation method thereof |
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2020
- 2020-01-16 CN CN202010044578.2A patent/CN111109083A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102674966A (en) * | 2012-05-21 | 2012-09-19 | 新疆生产建设兵团农六师农业科学研究所 | Special culture medium for potato virus-free seedling transplanting and preparation method thereof |
| CN103931500A (en) * | 2013-04-19 | 2014-07-23 | 云南农业大学 | Culture medium for promoting quick growth of potato tissue culture seedling |
| CN107148911A (en) * | 2017-03-29 | 2017-09-12 | 天津丰华裕隆农业发展有限公司 | A kind of sweet potato stem tip detoxification solid medium and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115843686A (en) * | 2022-12-03 | 2023-03-28 | 瑞盈优品(广东)农业科技有限责任公司 | Culture medium for sweet potato tissue culture and preparation method thereof |
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