CN107148911A - A kind of sweet potato stem tip detoxification solid medium and preparation method thereof - Google Patents
A kind of sweet potato stem tip detoxification solid medium and preparation method thereof Download PDFInfo
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- CN107148911A CN107148911A CN201710197539.4A CN201710197539A CN107148911A CN 107148911 A CN107148911 A CN 107148911A CN 201710197539 A CN201710197539 A CN 201710197539A CN 107148911 A CN107148911 A CN 107148911A
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- sweet potato
- potato stem
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention is a kind of sweet potato stem tip detoxification solid medium and preparation method thereof, is made up of the raw material of following parts by weight：100 200 parts of agar；I 43 58 parts of mother liquor；II 0.65 1.19 parts of mother liquor；36 parts of glycine；0.3 0.5 parts of thiamine hydrochloride；50 65 parts of inose；80 100 parts of sucrose；13 parts of bromocresol purple；0.2 0.4 parts of 3 acetic acid of indoles；PH value is 5.8；Its preparation method：Basal medium is configured to first；Complete medium is made again；Solid medium is prepared after adding agar stirring mixing, high pressure steam sterilization.The present invention is by adding bromocresol purple, it is easy to that solid medium pH value is adjusted, by adding sucrose, substantially increase stem apex emergence rate, by adding the acetic acid of indoles 3, the ability of stem apex formation callus is improved, growth of seedling is fast, so that cultivation cycle is greatly shortened, production cost has been saved.
Description
Technical field
The present invention relates to a kind of sweet potato technical field of tissue culture, more particularly to a kind of sweet potato stem tip detoxification solid culture
Base and preparation method thereof.
Background technology
Sweet potato stem tip detoxification tissue cultures refer to produce callus and again by nontoxic or detoxification the Stem tip induction of sweet potato
The process of plant is born, this process is mainly carried out on solid medium, but the pH value regulation of traditional solid medium
Inconvenience, when using traditional solid medium, emergence rate is low, due to the ability of stem apex formation callus, growth of seedling
Slowly, so that cultivation cycle is greatly prolonged, production cost is added.
The content of the invention
Present invention seek to address that the deficiencies in the prior art, and a kind of sweet potato stem tip detoxification solid medium and its system are provided
Preparation Method.
The present invention to achieve the above object, using following technical scheme：A kind of sweet potato stem tip detoxification solid medium, its
It is characterised by, is made up of the raw material of following parts by weight：
Preferably, the mother liquor I is made up of the raw material of following parts by weight：
Preferably, the mother liquor II is made up of the raw material of following parts by weight：
Preferably, the pH value is adjusted using sodium hydroxide and hydrochloric acid.
The present invention provides a kind of preparation method of sweet potato stem tip detoxification solid medium, it is characterised in that including following
Step：
(1) mother liquor I and mother liquor II are weighed according to formula respectively, and is mixed and is configured to basal medium；
(2) basal medium into step (1) by formula sequentially add glycine, thiamine hydrochloride, inose, sucrose,
Bromocresol purple and indole-3-acetic acid are configured to complete medium；
(3) complete medium into step (2) adds agar, and it is 5.8 to adjust the pH value of culture medium；
(4) mixing is stirred to culture medium at room temperature, packing is then carried out and goes forward side by side horizontal high voltage steam sterilizing, wherein sterilizing
Condition pressure is 103.0kPa, and temperature is 130 DEG C, and the reaction time is needed for preparing after 10-15min, high pressure steam sterilization
Sweet potato stem tip detoxification solid medium.
The beneficial effects of the invention are as follows：The present invention is easy to adjust solid medium pH value by adding bromocresol purple
Section, by adding sucrose, substantially increases stem apex emergence rate, by adding indole-3-acetic acid, improves stem apex formation callus group
The ability knitted, growth of seedling is fast, so that cultivation cycle is greatly shortened, has saved production cost.
Embodiment
With reference to embodiment, the invention will be further described：
Embodiment 1
A kind of sweet potato stem tip detoxification solid medium, is made up of the raw material of following parts by weight：
Preparation method：A kind of Sweet Potato provided exemplified by preparing sweet potato stem tip detoxification solid medium the present embodiment
Sharp detoxification is described below with the preparation method of solid medium：
(1) 10 parts of potassium nitrate, 25 parts of ammonium nitrate, 5 parts of magnesium sulfate, 3 parts of calcium chloride, 0.01 part of iodine are weighed according to formula respectively
Change potassium, 0.02 part of copper sulphate, 0.4 part of disodium ethylene diamine tetraacetate, 0.02 part of cobalt chloride, 0.2 part of ferrous sulfate, and be mixed
It is configured to basal medium；
(2) basal medium into step (1) sequentially adds 3 parts of glycine, 0.3 part of thiamine hydrochloride, 50 by formula
Part inose, 80 portions of sucrose, 1 part of bromocresol purple and 0.2 part of indole-3-acetic acid are configured to complete medium；
(3) complete medium into step (2) adds 100 parts of agar, and it is 5.8 to adjust the pH value of culture medium；
(4) culture medium is stirred after mixing at room temperature, then carries out packing and go forward side by side horizontal high voltage steam sterilizing, wherein going out
Bacterium condition pressure is 103.0kPa, and temperature is 130 DEG C, and the reaction time is needed for preparing after 10min, high pressure steam sterilization
Sweet potato stem tip detoxification solid medium.
Embodiment 2
A kind of sweet potato stem tip detoxification solid medium, is made up of the raw material of following parts by weight：
Preparation method：A kind of Sweet Potato provided exemplified by preparing sweet potato stem tip detoxification solid medium the present embodiment
Sharp detoxification is described below with the preparation method of solid medium：
(1) 15 parts of potassium nitrate, 30 parts of ammonium nitrate, 8 parts of magnesium sulfate, 5 parts of calcium chloride, 0.03 part of iodine are weighed according to formula respectively
Change potassium, 0.04 part of copper sulphate, 0.7 part of disodium ethylene diamine tetraacetate, 0.02 part of cobalt chloride, 0.4 part of ferrous sulfate, and be mixed
It is configured to basal medium；
(2) basal medium into step (1) sequentially adds 6 parts of glycine, 0.5 part of thiamine hydrochloride, 65 by formula
Part inose, 100 portions of sucrose, 3 parts of bromocresol purples and 0.4 part of indole-3-acetic acid are configured to complete medium；
(3) complete medium into step (2) adds 200 parts of agar, and it is 5.8 to adjust the pH value of culture medium；
(4) mixing is stirred to culture medium at room temperature, packing is then carried out and goes forward side by side horizontal high voltage steam sterilizing, wherein sterilizing
Condition pressure is 103.0kPa, and temperature is 130 DEG C, and the reaction time is sweet needed for preparing after 15min, high pressure steam sterilization
Potato stem apex detoxification solid medium.
The present invention is exemplarily described above, it is clear that the present invention, which is implemented, to be not subject to the restrictions described above,
As long as employing the various improvement of inventive concept and technical scheme of the present invention progress, or not improved directly apply to other fields
Close, within protection scope of the present invention.
Claims (5)
1. a kind of sweet potato stem tip detoxification solid medium, it is characterised in that be made up of the raw material of following parts by weight：
2. a kind of sweet potato stem tip detoxification solid medium according to claim 1, it is characterised in that the mother liquor I by
The raw material composition of following parts by weight：
3. a kind of sweet potato stem tip detoxification solid medium according to claim 1, it is characterised in that the mother liquor II by
The raw material composition of following parts by weight：
4. a kind of sweet potato stem tip detoxification solid medium according to claim 1, it is characterised in that the pH value is used
Sodium hydroxide and hydrochloric acid are adjusted.
5. a kind of preparation method of sweet potato stem tip detoxification solid medium as claimed in claim 1, it is characterised in that including
Following steps：
(1) mother liquor I and mother liquor II are weighed according to formula respectively, and is mixed and is configured to basal medium；
(2) basal medium into step (1) sequentially adds glycine, thiamine hydrochloride, inose, sucrose, bromine first by formula
Phenol violet and indole-3-acetic acid are configured to complete medium；
(3) complete medium into step (2) adds agar, and it is 5.8 to adjust the pH value of culture medium；
(4) mixing is stirred to culture medium at room temperature, packing is then carried out and goes forward side by side horizontal high voltage steam sterilizing, wherein sterilising conditions
Pressure is 103.0kPa, and temperature is 130 DEG C, and the reaction time is sweet needed for preparing after 10-15min, high pressure steam sterilization
Potato stem apex detoxification solid medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710197539.4A CN107148911A (en) | 2017-03-29 | 2017-03-29 | A kind of sweet potato stem tip detoxification solid medium and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710197539.4A CN107148911A (en) | 2017-03-29 | 2017-03-29 | A kind of sweet potato stem tip detoxification solid medium and preparation method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN107148911A true CN107148911A (en) | 2017-09-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710197539.4A Pending CN107148911A (en) | 2017-03-29 | 2017-03-29 | A kind of sweet potato stem tip detoxification solid medium and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110810247A (en) * | 2019-12-05 | 2020-02-21 | 天津丰华裕隆农业发展股份有限公司 | Sweet potato stem tip detoxification and breeding method |
| CN111011216A (en) * | 2019-12-31 | 2020-04-17 | 青海省农林科学院 | Efficient regeneration culture medium and culture method for Atlantic potatoes |
| CN111109083A (en) * | 2020-01-16 | 2020-05-08 | 河南华薯农业科技有限公司 | Culture medium suitable for cultivating multiple sweet potato seedlings and preparation method thereof |
-
2017
- 2017-03-29 CN CN201710197539.4A patent/CN107148911A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110810247A (en) * | 2019-12-05 | 2020-02-21 | 天津丰华裕隆农业发展股份有限公司 | Sweet potato stem tip detoxification and breeding method |
| CN111011216A (en) * | 2019-12-31 | 2020-04-17 | 青海省农林科学院 | Efficient regeneration culture medium and culture method for Atlantic potatoes |
| CN111109083A (en) * | 2020-01-16 | 2020-05-08 | 河南华薯农业科技有限公司 | Culture medium suitable for cultivating multiple sweet potato seedlings and preparation method thereof |
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Application publication date: 20170912 |