CN111108842A - Peucedanum praeruptorum seed treatment method - Google Patents
Peucedanum praeruptorum seed treatment method Download PDFInfo
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- A—HUMAN NECESSITIES
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- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/58—1,2-Diazines; Hydrogenated 1,2-diazines
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
- A01N47/14—Di-thio analogues thereof
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- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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Abstract
The invention is suitable for the technical field of seed treatment, and provides a method for treating seeds of peucedanum praeruptorum, which comprises the following steps: selecting seeds positioned in the middle section area of the peucedanum praeruptorum as seeds; soaking the seeds in an inhibiting solution for a preset time, fishing out and cleaning with clear water; and storing the seeds washed by clear water in a refrigeration house at the temperature of between 5 ℃ below zero and 5 ℃. The seeds in the middle section area of the peucedanum praeruptorum are selected as the seeds, so that the nutrition content in the seeds can be basically the same, the growth vigor of the seeds after sowing is ensured to be the same, the seeds are soaked in the inhibiting solution and stored in the refrigeration house at the temperature of minus 5 to 5 ℃, so that the seeds can enter a dormant state, the vitality of the seeds is kept, the germination rate of the seeds can be improved when the seeds are taken out for sowing in the second or third year, and the seeds do not need to be soaked by medicines before sowing.
Description
Technical Field
The invention belongs to the technical field of seed treatment, and particularly relates to a method for treating seeds of peucedanum praeruptorum.
Background
Radix Peucedani is a plant of Umbelliferae, has high essential value at root, has main functions of lowering qi, eliminating phlegm, dispelling pathogenic wind, clearing heat, etc., and is mainly used for treating phlegm-heat cough and asthma, wind-heat cough, etc. Therefore, the demand of the peucedanum praeruptorum is large, and the planting technology thereof is still perfected. Good varieties are selected for better yield, and seed harvesting and storage are important.
The seeds are obtained from the mature period of the peucedanum praeruptorum to the planting place and the selected seeds need to have certain activity to ensure the germination rate. The seeds need to be stored after being obtained, so that the seeds can be sown when the seeds need to be sown in the second year or the third year.
However, the existing peucedanum praeruptorum seeds are not stored well, and most seeds are taken out and sowed in the second or third year, so that the problems of low germination rate and different growth vigors occur, and a great amount of resources are wasted.
Disclosure of Invention
The embodiment of the invention provides a method for treating seeds of peucedanum praeruptorum, aiming at solving the problems of low germination rate and different growth vigor of the peucedanum praeruptorum seeds.
The embodiment of the invention is realized in such a way, and provides a method for treating seeds of peucedanum praeruptorum, which comprises the following steps:
selecting seeds positioned in the middle section area of the peucedanum praeruptorum as seeds;
soaking the seeds in an inhibiting solution for a preset time, fishing out and cleaning with clear water;
and storing the seeds washed by clear water in a refrigeration house at the temperature of between 5 ℃ below zero and 5 ℃.
Further, the seeds are stored in a freezer at 0 to 5 ℃.
Further, the seeds are stored in a freezer at 5 ℃.
Further, the illumination time of the seeds after being stored in a cold storage is less than 0.1 h/d.
Further, the relative humidity in the refrigerator is 20% to 30%.
Further, the inhibiting solution is one of a paclobutrazol solution, an abscisic acid solution, and a cynarin solution.
Furthermore, the step of selecting the kernel in the midsection area of the peucedanum root as the seed further comprises:
the grain size of the selected grains is 2.5-3 mm.
Furthermore, the method also comprises the following steps of soaking the seeds in the inhibiting solution for a preset time, fishing out the seeds and washing the seeds with clean water, and storing the seeds washed with clean water in a refrigeration house with the temperature of-5 to 5 ℃:
soaking the cleaned seeds in a sodium hydroxide solution with the concentration of 1.5-2.5% for 15-25 minutes, taking out and cleaning with clear water.
The seeds in the middle section area of the peucedanum praeruptorum are selected as the seeds, so that the nutrition content in the seeds can be basically the same, the growth vigor of the seeds after sowing is ensured to be the same, the seeds are soaked in the inhibiting solution and stored in the refrigeration house at the temperature of minus 5 to 5 ℃, so that the seeds can enter a dormant state, the vitality of the seeds is kept, the germination rate of the seeds can be improved when the seeds are taken out for sowing in the second or third year, and the seeds do not need to be soaked by medicines before sowing.
Drawings
Fig. 1 is a schematic diagram of a seed treatment method for peucedanum root according to an embodiment of the present invention.
Fig. 2 is a schematic diagram of another method for treating seeds of peucedanum root according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example one
The embodiment provides a method for treating seeds of peucedanum praeruptorum, as shown in fig. 1, the method comprises the following steps:
and S11, selecting seeds positioned in the middle section area of the peucedanum praeruptorum as seeds.
Selecting seeds from a planting place of the peucedanum praeruptorum in 11-12 months, selecting a seed canopy in the middle section area of the peucedanum praeruptorum, then drying in the sun, rubbing the seed canopy to drop the seeds, and screening the seeds which are full or have 65-85% of fullness as the seeds, wherein the selected seeds have no disease spots and are not damaged.
The seeds in the central region of the peucedanum praeruptorum are selected mainly because the nutrition content of the seeds in the central region of the peucedanum praeruptorum is basically the same and the difference is not large, so that the seeds have basically the same growth vigor after being planted, and the later-stage management is convenient.
Meanwhile, the nutrient content in the seeds in the middle section area of the peucedanum praeruptorum is between full and not full, the growth vigor of the peucedanum praeruptorum seeds is relatively slow after planting, and the bolting condition is not reached by the bolting period, so that the bolting quantity can be reduced, the lignification of the peucedanum praeruptorum is reduced, and the A and the B in the peucedanum praeruptorum reach the pharmaceutical standard.
S12, soaking the seeds in the inhibiting solution for a preset time, fishing out and cleaning with clear water.
The main function of the inhibiting solution (also called dormancy agent, inhibitor, dormancy solution, inhibiting solution, etc.) is to inhibit the germination of seeds or to promote the seeds to enter dormancy.
The inhibiting solution may be one of paclobutrazol solution, abscisic acid solution, and cynarin solution.
When the seeds are soaked by the paclobutrazol solution, the preset soaking time is 1 to 2 hours, of course, 150ppm, 400ppm or 250ppm of the paclobutrazol solution can be used according to actual requirements, and the corresponding soaking time can be adjusted, such as soaking for 40 minutes, 41 minutes, 3 hours, 4 hours and the like.
When the abscisic acid solution is used for soaking seeds, the preset soaking time is 3 to 4 hours, of course, according to actual requirements, 130ppm, 300ppm or 200ppm abscisic acid solution can be used, and the corresponding soaking time can be adjusted, such as soaking for 1.5 hours, 2 hours, 5 hours, 6 hours, and the like.
When the seeds are soaked in the solution of the cynanchum glaucescens, the preset time for soaking is 40-90 hours, of course, 3000ppm, 4000ppm or 5000ppm of the solution of the cynanchum glaucescens can be used according to actual requirements, and the corresponding soaking time can be adjusted, such as 30 minutes, 1.5 hours, 2 hours, 3 hours and the like.
The inhibiting solution can also be triiodobenzoic acid solution, chlormequat chloride solution, etc.
After the seeds are washed by clean water, the water on the seeds needs to be sucked dry or dried in the sun or dried to avoid the water content on the surfaces of the seeds, and if the water content on the surfaces of the seeds is caused, the seeds are frozen in a refrigeration house and even directly cause the inactivation of the seeds.
The seeds can be absorbed by absorbent paper after being dried, and the seeds can be placed in the sun for airing (without exposure) after being dried, and an oven can be used for drying.
S13, storing the seeds washed by clean water in a refrigeration house at the temperature of between 5 ℃ below zero and 5 ℃.
Of course, the seeds can also be stored in refrigerators, freezers, cold stores at-5 to 5 ℃.
The storage temperature can also be adjusted properly, such as in a refrigerator at-6 deg.C, -3 deg.C, 2 deg.C, 6 deg.C, 7 deg.C.
The cold storage has ventilation conditions, such as an air interchanger or a window and the like, so that the cold storage can be ventilated, and seeds are prevented from mildewing.
In the embodiment, the seeds in the middle section area of the peucedanum praeruptorum are selected as the seeds, so that the nutrient contents in the seeds are basically the same, the same growth vigor of the seeds after sowing is ensured, the seeds are soaked in the inhibiting solution and stored in the refrigeration house at the temperature of-5 to 5 ℃, so that the seeds can enter a dormant state to keep the vitality of the seeds, the germination rate of the seeds can be improved when the seeds are taken out for sowing in the second year or the third year, and the seeds do not need to be soaked by medicines before sowing.
As another alternative embodiment of the invention, the seeds are stored in a freezer at 0 to 5 ℃.
Specifically, the storage temperature can be fixed at 0 ℃, 2 ℃, 3 ℃ and 4 ℃, and the obtained effect is different.
In the embodiment, the seeds are stored in the refrigeration storage at 0-5 ℃, so that the vitality of the seeds can be further protected, and the condition that the vitality of the seeds is reduced due to too low storage temperature of the seeds, so that the seeds cannot germinate, is avoided.
As another alternative embodiment of the invention, the seeds are stored in a freezer at 5 ℃.
In the embodiment, the seeds are stored in the refrigeration storage at 5 ℃, so that the vitality of the seeds can be further protected, and the condition that the vitality of the seeds is reduced and the seeds cannot germinate due to too low storage temperature of the seeds is avoided.
As another alternative embodiment of the invention, the illumination time of the seeds after being stored in the cold storage is less than 0.1h/d (hours per day).
Specifically, the seed is placed back in the freezer, can avoid receiving illumination through covering the puggaree, also can directly place the seed in the corner of freezer dark to avoid illumination.
The preferable illumination time length is 0h/d, and of course, the illumination time length can be adjusted according to the actual situation, such as the illumination time length is 0.2h/d, 0.3h/d, 0.4h/d, and the like.
According to the embodiment, the illumination time of the seeds is less than 0.1h/d, so that the seeds can be prevented from reaching the illumination condition in a refrigeration house, and the seeds can germinate in advance.
As another alternative embodiment of the present invention, the relative humidity in the refrigerator is 20% to 30%.
Of course, the relative humidity can be adjusted, such as to 15%, 16%, 24%, 25%, 31%, 32%, etc.
This embodiment is through the relative humidity who restricts in the freezer, and humidity in can avoiding the freezer is overweight, leads to the seed to go mildy, simultaneously, can also avoid the humidity overweight in the freezer, impels the seed to sprout in advance.
As another optional embodiment of the present invention, the step S11 of selecting the kernel located in the midstream region of the peucedanum root as the seed further includes:
the grain size of the selected grains is 2.5-3 mm.
The seeds with the particle size of 2.5 to 3mm can be screened by two seed sieves with the sieve pores of 2.5mm and the sieve pores of 3mm, so that the seeds with the fixed particle size can be quickly obtained.
In the embodiment, the seeds with the grain diameter of 2.5-3 mm are selected as the seeds, so that the same growth vigor of the seeds after planting can be ensured, meanwhile, the nutrient content in the seeds within the grain diameter range is relatively in the middle period of the peucedanum seeds, the growth vigor is relatively slow after planting, and the bolting condition is not met by the seeds in the bolting period, so that the bolting quantity can be reduced, the lignification of the peucedanum is reduced, and the A and B in the peucedanum meet the pharmaceutical standard.
Carry out two
The embodiment provides a method for treating seeds of peucedanum praeruptorum, as shown in fig. 2, the method comprises the following steps:
and S21, selecting seeds positioned in the middle section area of the peucedanum praeruptorum as seeds.
S22, soaking the seeds in the inhibiting solution for a preset time, fishing out and cleaning with clear water.
S23, soaking the cleaned seeds in a sodium hydroxide solution with the concentration of 1.5-2.5% for 15-25 minutes, taking out and cleaning with clear water.
S24, storing the seeds washed by clean water in a refrigeration house at the temperature of between 5 ℃ below zero and 5 ℃.
The sodium hydroxide in the embodiment is mainly used for sterilization, so the sodium hydroxide in the embodiment can also be replaced by other bactericides, such as 50% carbendazim, potassium permanganate solution, amobam and the like, and the soaking time is adjusted according to the selected bactericides.
In the embodiment, by soaking the seeds with the 1.5-2.5% sodium hydroxide solution, the seeds can be in an aseptic state before storage, so that the storage time of the seeds is prolonged, and the effect of prevention can be achieved after sowing.
In order to better embody the beneficial effects brought by the present invention, the following experiments and data of the germination percentage of the peucedanum praeruptorum seeds after planting are described:
purpose of the experiment: and acquiring the germination rate of the peucedanum praeruptorum seeds.
The experimental scheme is as follows: the experimental groups of the scheme are seven groups, and the scheme specifically comprises the following steps:
the first set of experiments: the research field is one mu; the planted seeds are seeds stored by a conventional method (no medicament is soaked, the storage temperature is 5-9 ℃, and the medicament soaking breaks the dormancy period); the germination rate of the study area was calculated.
The second set of experiments: the research field is one mu; the seeds planted were the seeds of the present invention (soaked with paclobutrazol solution, stored at-5 to 5 ℃, sowed directly), and the germination rate in the study area was calculated.
The third set of experiments: the research field is one mu; the seeds planted were the seeds of the present invention (soaked with the cynarin, stored at-5 to 5 ℃, directly sown), and the germination rate in the study area was calculated.
Fourth set of experiments: the research field is one mu; the seeds planted were the seeds of the present invention (soaked with paclobutrazol solution, stored at 0 to 5 ℃, sown directly), and the germination rate in the study area was calculated.
Fifth set of experiments: the research field is one mu; the seeds planted were the seeds of the present invention (soaked with the cynarin, stored at 0 to 5 ℃, directly sown), and the germination rate in the study area was calculated.
The sixth set of experiments: the research field is one mu; the seeds planted were the seeds of the present invention (soaked with paclobutrazol solution, stored at 5 ℃, sown directly), and the germination percentage in the study area was calculated.
The seventh set of experiments: the research field is one mu; the seeds planted were the seeds of the present invention (soaked with the cynarin, stored at 5 ℃, sowed directly), and the germination percentage in the study area was calculated.
The planting time, planting conditions (water and fertilizer content in the planting field, the length of illumination time and the like) and the management mode after planting (fertilizing time, fertilizing amount, weeding time and the like) in the seven groups of experiments are the same or similar, and specific data in the seven groups of experiments are shown in table 1:
TABLE 1
| Experimental group | Germination percentage in first year (%) | Germination percentage in the second year (%) |
| First group | 70.67 | 68.43 |
| Second group | 94.65 | 93.35 |
| Third group | 96.46 | 92.40 |
| Fourth group | 97.28 | 95.47 |
| Fifth group | 96.72 | 96.43 |
| Sixth group | 97.87 | 94.10 |
| Seventh group | 98.09 | 95.44 |
From the above table it is clear that the average first-year germination rate of the seeds stored using the method of the present invention is 96.85%, which is a significant improvement of 26.18% over the germination rate of the seeds stored in the prior art storage method. The average germination rate in the second year is 94.53%, which is obviously improved by 26.10% compared with the germination rate of the seeds stored by the existing storage method, therefore, the germination rate of the seeds stored by the method of the invention is obviously improved compared with the germination rate of the seeds stored by the existing storage method.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (8)
1. A method for treating seeds of peucedanum praeruptorum, which is characterized by comprising the following steps:
selecting seeds positioned in the middle section area of the peucedanum praeruptorum as seeds;
soaking the seeds in an inhibiting solution for a preset time, fishing out and cleaning with clear water;
and storing the seeds washed by clear water in a refrigeration house at the temperature of between 5 ℃ below zero and 5 ℃.
2. The method for seed treatment of peucedanum praeruptorum dunn of claim 1, wherein the seeds are stored in a freezer at 0 to 5 ℃.
3. The method for seed treatment of peucedanum praeruptorum dunn of claim 1, wherein the seeds are stored in a freezer at 5 ℃.
4. The method for treating seeds of peucedanum praeruptorum dunn of claim 1, wherein the illumination time of the seeds after being stored in the refrigerator is less than 0.1 h/d.
5. The method for seed treatment of peucedanum praeruptorum dunn of claim 1, wherein the relative humidity in said freezer is 20% to 30%.
6. The method for seed treatment of peucedanum praeruptorum dunn according to any one of claims 1 to 5, wherein the inhibiting solution is one of paclobutrazol solution, abscisic acid solution, and cynarin solution.
7. The method of any of claims 1 to 5, wherein the step of selecting seeds in the midsection region of the peucedanum root as seeds further comprises:
the grain size of the selected grains is 2.5-3 mm.
8. The method for treating seeds of peucedanum praeruptorum dunn according to claim 1, wherein the method for treating seeds by soaking the seeds in the inhibition solution for a preset time, fishing out the seeds and washing the seeds with clean water, and before storing the seeds washed with clean water in a refrigerator at-5 to 5 ℃, further comprises the steps of:
soaking the cleaned seeds in a sodium hydroxide solution with the concentration of 1.5-2.5% for 15-25 minutes, taking out and cleaning with clear water.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911277002.4A CN111108842B (en) | 2019-12-12 | 2019-12-12 | Peucedanum praeruptorum seed treatment method |
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| CN201911277002.4A CN111108842B (en) | 2019-12-12 | 2019-12-12 | Peucedanum praeruptorum seed treatment method |
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| CN111108842B CN111108842B (en) | 2021-12-31 |
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