CN111072427A - Composite microbial fertilizer for preventing and treating apple pockmark disease and preparation method thereof - Google Patents
Composite microbial fertilizer for preventing and treating apple pockmark disease and preparation method thereof Download PDFInfo
- Publication number
- CN111072427A CN111072427A CN201911335501.4A CN201911335501A CN111072427A CN 111072427 A CN111072427 A CN 111072427A CN 201911335501 A CN201911335501 A CN 201911335501A CN 111072427 A CN111072427 A CN 111072427A
- Authority
- CN
- China
- Prior art keywords
- parts
- microbial fertilizer
- bacillus subtilis
- weight
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to a compound microbial fertilizer for preventing and treating apple pockmark disease and a preparation method thereof, wherein the compound microbial fertilizer comprises the following components in parts by weight: 1-5 parts of bacillus subtilis fermentation liquor, 20-30 parts of sugar alcohol calcium, 5 parts of monopotassium phosphate, 15-30 parts of ammonium fulvate, 5 parts of boric acid and 40-60 parts of water; uniformly mixing the prepared bacillus subtilis fermentation liquor with the components to prepare the composite microbial fertilizer; the compound microbial fertilizer can effectively increase the calcium content in the pulp of mature apples, effectively prevent and treat apple pockmark diseases, is beneficial to the stable preservation of apples, ensures the quality of apples and increases the economic benefit of growers; the compound microbial fertilizer can play a role of a fertilizer and simultaneously adjust the balance of soil flora, thereby promoting the absorption and utilization of calcium by fruit trees.
Description
Technical Field
The invention relates to a compound microbial fertilizer for preventing and treating apple pockmark disease and a preparation method thereof, belonging to the technical field of microbial fertilizers.
Technical Field
The apple bitter pox disease is also called as a bitter pit disease, is mainly caused by physiological calcium deficiency of a tree body, is a physiological disease which is commonly generated in the mature period and the storage period of an apple, is mainly reflected on fruits, and the symptoms begin to appear when the fruits are nearly mature, and continue to develop in the storage period.
China is a traditional big agricultural country, agriculture is a fundamental industry of China, and plays a significant role in national economy. The living standard of people is continuously improved, the requirements on products provided by agriculture are correspondingly improved, especially the demand on fruits such as apples is increased year by year, the northern part of China is the main planting area of apples, and the bitter pox disease is also called as a bitter cave disease and a spot disease, is a calcium deficiency physiological disease which is frequently seen in the mature period and the storage period of apples, and is mainly reflected on the fruits. The apple anthracnose becomes a main disease affecting the economic character of apples worldwide, the disease is increasingly serious after bagging cultivation is carried out in China, the disease rate of fruits in some orchards is up to more than 50%, and the improvement of the quality and the economic benefit of the fruits is seriously affected. The traditional secondary element water-soluble fertilizer, inorganic calcium fertilizer and the like have the conditions of difficult absorption, poor effect and the like.
Chinese patent document CN108929169A (application No. 201811007512.5) discloses an environment-friendly disease-resistant apple tree fertilizer, which comprises corn straws, potassium fulvate, furfural residues, monopotassium phosphate, sodium tetraborate, kaolin, sodium citrate, castor cakes, bone meal, activated carbon, zeolite powder and composite strain powder. The fertilizer prepared by the invention is durable, the utilization rate of the fertilizer is high, the resistance of apple trees is effectively improved, the proportion is reasonable, the fertilizer is economic and environment-friendly, but the fertilizer still has the defect that calcium is difficult to absorb, and apple bitter pox disease cannot be prevented.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a compound microbial fertilizer for preventing and treating apple bitter pox and a preparation method thereof.
The compound microbial fertilizer can effectively increase the calcium content in the pulp of mature apples, effectively prevent and treat apple pockmark diseases, is beneficial to the stable preservation of apples, ensures the quality of apples and increases the economic benefit of growers; the compound microbial fertilizer can play a role of a fertilizer and simultaneously adjust the balance of soil flora, thereby promoting the absorption and utilization of calcium by fruit trees.
The technical scheme of the invention is as follows:
the compound microbial fertilizer for preventing and treating apple pockmark disease comprises the following components in parts by weight:
1-5 parts of bacillus subtilis fermentation liquor, 20-30 parts of sugar alcohol calcium, 5 parts of monopotassium phosphate, 15-30 parts of ammonium fulvate, 5 parts of boric acid and 40-60 parts of water;
the effective viable count of the bacillus subtilis in the fermentation liquor is more than or equal to 250 hundred million/ml.
According to the invention, the compound microbial fertilizer preferably comprises the following components in parts by weight: 5 parts of bacillus subtilis fermentation liquor, 30 parts of sugar alcohol calcium, 5 parts of monopotassium phosphate, 30 parts of ammonium fulvate, 5 parts of boric acid and 60 parts of water; the effective viable count of the bacillus subtilis in the fermentation liquid is 350 hundred million/ml.
According to the invention, preferably, the bacillus subtilis (Bacillus subtilis) in the bacillus subtilis fermentation liquor is derived from China agricultural microbial strain preservation management center with strain number ACCC 10242.
The preparation method of the compound microbial fertilizer comprises the following steps:
(1) activating bacillus subtilis by a slant culture medium, transferring the activated bacillus subtilis into a liquid seed culture medium, performing shake culture to obtain a liquid seed A, inoculating the prepared liquid seed A into a liquid fermentation culture medium, regulating the pH value to 7.0-8.0, regulating the aeration ratio to be 1 (0.5-1) and the rotation speed to be 100-200 rpm, and culturing at 30-37 ℃ for 36-48 hours to obtain a fermentation liquid, wherein the effective viable count of the bacillus subtilis in the fermentation liquid is more than or equal to 250 hundred million/ml;
the slant culture conditions in the step (1) are as follows: culturing in a slant culture medium at the temperature of 30-37 ℃ for 24-48 h; the slant culture medium comprises the following components per liter:
6-9 g of peptone, 3-5 g of yeast powder, 2-4 g of beef extract, 3-5 g of sodium chloride, 15-25 g of agar, 0.5-1 g of monopotassium phosphate, 0.5-1 g of dipotassium phosphate and the balance of water;
the shake flask culture conditions in the step (1) are as follows: culturing for 36-48 h at 30-37 ℃; the liquid seed culture medium comprises the following components per liter:
6-9 g of peptone, 5-8 g of glucose, 3-5 g of yeast powder, 2-4 g of beef extract, 3-5 g of sodium chloride, 0.5-1 g of monopotassium phosphate, 0.5-1 g of dipotassium phosphate and the balance of water;
the liquid fermentation medium in the step (1) comprises the following components per liter:
10-15 g of corn flour, 10-15 g of bean cake powder, 5-10 g of glucose, 5-10 g of ammonium sulfate, 3-5 g of yeast powder, 0.5-0.8 g of monopotassium phosphate, 0.1-0.25 g of magnesium sulfate, 0.1-0.25 g of manganese sulfate, 0.1-0.2 g of ferrous chloride and the balance of water, and adjusting the pH value to 7-8;
(2) uniformly mixing the bacillus subtilis fermentation broth prepared in the step (1), sugar alcohol calcium, monopotassium phosphate, ammonium fulvate, boric acid and water in parts by weight to prepare a composite microbial fertilizer;
(3) and (3) performing 150-mesh screening on the composite microbial fertilizer prepared in the step (2), removing particulate impurities larger than 150 meshes, facilitating drip irrigation application and avoiding blocking a drip irrigation pipe head.
Preferably, the effective viable count of the fermentation liquor prepared in the step (1) is 350 hundred million/ml; and (2) uniformly mixing 5 parts by weight of the bacillus subtilis fermentation liquid prepared in the step (1), 30 parts by weight of sugar alcohol calcium, 5 parts by weight of monopotassium phosphate, 30 parts by weight of ammonium fulvate, 5 parts by weight of boric acid and 60 parts by weight of water to prepare the composite microbial fertilizer.
The compound microbial fertilizer is applied to preventing and treating apple pockmark disease.
The beneficial effect of the technical scheme
1. The compound microbial fertilizer provided by the invention can effectively increase the calcium content of mature apple pulp, effectively prevent and treat apple pockmark disease, is beneficial to stable storage of apples, ensures the quality of apples, and increases the economic benefit of growers.
2. The compound microbial fertilizer provided by the invention can play a role of a fertilizer, and can also adjust the balance of soil flora and promote the absorption and utilization of calcium by fruit trees.
Detailed Description
The technical solution of the present invention is further described with reference to the following examples, but the scope of the present invention is not limited thereto.
Microbial origin
Bacillus subtilis (Bacillus subtilis) is purchased from China agricultural microorganism strain preservation management center with the strain number ACCC 10242;
bacillus subtilis No. LZH-H1 was purchased from Dezhou institute of Biophysical stress laboratory.
Bacillus subtilis No. LZH-13 was purchased from Dezhou institute of Biophysical stress laboratory.
Example 1
The compound microbial fertilizer for preventing and treating apple pockmark disease comprises the following components in parts by weight:
2 parts of bacillus subtilis (ACCC10242) fermentation liquor, 20 parts of sugar alcohol calcium, 5 parts of monopotassium phosphate, 15 parts of ammonium fulvate, 5 parts of boric acid and 40 parts of water; the effective viable count of the bacillus subtilis in the fermentation liquid is 270 hundred million/ml.
The preparation method of the compound microbial fertilizer for preventing and treating apple pockmark disease comprises the following steps:
(1) activating bacillus subtilis (ACCC10242) by a slant culture medium, transferring the activated bacillus subtilis (ACCC10242) into a liquid seed culture medium, performing shake culture to obtain a liquid seed A, inoculating the prepared liquid seed A into a liquid fermentation culture medium, regulating the pH value to 7.0, regulating the aeration ratio to be 1:0.5, rotating speed to be 100 revolutions per minute, culturing at the temperature of 30 ℃ for 46 hours to obtain a fermentation liquid, wherein the effective viable count of the bacillus subtilis in the fermentation liquid is 270 hundred million/ml;
the slant culture conditions in the step (1) are as follows: culturing at 35 deg.C in slant culture medium for 36 h; the slant culture medium comprises the following components per liter:
8g of peptone, 3g of yeast powder, 2g of beef extract, 5g of sodium chloride, 17g of agar, 0.5g of monopotassium phosphate, 0.5g of dipotassium phosphate and the balance of water;
the shake flask culture conditions in the step (1) are as follows: culturing at 35 deg.C for 42 h; the liquid seed culture medium comprises the following components per liter:
6g of peptone, 5g of glucose, 3g of yeast powder, 2g of beef extract, 3g of sodium chloride, 0.5g of monopotassium phosphate, 1g of dipotassium phosphate and the balance of water;
the liquid fermentation medium in the step (1) comprises the following components per liter:
10g of corn flour, 10g of bean cake powder, 5g of glucose, 5g of ammonium sulfate, 3g of yeast powder, 0.5g of monopotassium phosphate, 0.2g of magnesium sulfate, 0.1g of manganese sulfate, 0.1g of ferrous chloride and the balance of water, and the pH value is adjusted to be 7.0.
(2) And (2) putting the bacillus subtilis fermentation liquor prepared in the step (1), sugar alcohol calcium, monopotassium phosphate, ammonium fulvate, boric acid and water in the mixing tank according to the weight parts, uniformly stirring for 30 minutes, and thus obtaining the compound microbial fertilizer.
(3) And (3) feeding the compound microbial fertilizer prepared in the step (2) into a vibrating screen of 150 meshes for screening, and removing particulate impurities larger than 150 meshes, so that drip irrigation application is facilitated, and a drip irrigation pipe head is prevented from being blocked.
(4) And (4) packaging the compound microbial fertilizer screened in the step (3).
Example 2
The compound microbial fertilizer for preventing and treating apple pockmark disease comprises the following components in parts by weight:
5 parts of bacillus subtilis (ACCC10242) fermentation liquor, 25 parts of sugar alcohol calcium, 5 parts of monopotassium phosphate, 20 parts of ammonium fulvate, 5 parts of boric acid and 50 parts of water; the effective viable count of the bacillus subtilis in the fermentation liquid is 300 hundred million/ml.
The preparation method of the compound microbial fertilizer comprises the following steps:
(1) activating bacillus subtilis (ACCC10242) by a slant culture medium, transferring the activated bacillus subtilis (ACCC10242) into a liquid seed culture medium, performing shake culture to obtain a liquid seed A, inoculating the prepared liquid seed A into a liquid fermentation culture medium, regulating the pH value to 7.5, regulating the aeration ratio to be 1:0.75, controlling the rotating speed to be 150 revolutions per minute, controlling the culture temperature to be 37 ℃, and controlling the culture time to be 36 hours to obtain a fermentation liquid, wherein the effective viable count of the bacillus subtilis in the fermentation liquid is 300 hundred million/ml;
the slant culture conditions in the step (1) are as follows: culturing at 37 deg.C in slant culture medium for 40 h; the slant culture medium comprises the following components per liter:
8g of peptone, 4g of yeast powder, 3g of beef extract, 4g of sodium chloride, 17g of agar, 0.8g of monopotassium phosphate, 0.8g of dipotassium phosphate and the balance of water;
the shake flask culture conditions in the step (1) are as follows: culturing at 35 deg.C for 42 h; the liquid seed culture medium comprises the following components per liter:
9g of peptone, 5g of glucose, 5g of yeast powder, 2g of beef extract, 5g of sodium chloride, 0.5g of monopotassium phosphate, 1g of dipotassium phosphate and the balance of water;
the liquid fermentation medium in the step (1) comprises the following components per liter:
12g of corn flour, 13g of bean cake powder, 8g of glucose, 6g of ammonium sulfate, 4g of yeast powder, 0.7g of monopotassium phosphate, 0.2g of magnesium sulfate, 0.15g of manganese sulfate, 0.15g of ferrous chloride and the balance of water, and the pH value is adjusted to be 7.4.
(2) And (2) putting the bacillus subtilis fermentation liquor prepared in the step (1), sugar alcohol calcium, monopotassium phosphate, ammonium fulvate, boric acid and water in the mixing tank according to the weight parts, uniformly stirring for 35 minutes, and thus obtaining the compound microbial fertilizer.
(3) And (3) feeding the compound microbial fertilizer prepared in the step (2) into a vibrating screen of 150 meshes for screening, and removing particulate impurities larger than 150 meshes, so that drip irrigation application is facilitated, and a drip irrigation pipe head is prevented from being blocked.
(4) And (4) packaging the compound microbial fertilizer screened in the step (3).
Example 3
The compound microbial fertilizer for preventing and treating apple pockmark disease comprises the following components in parts by weight:
5 parts of bacillus subtilis (ACCC10242) fermentation liquor, 30 parts of sugar alcohol calcium, 5 parts of monopotassium phosphate, 30 parts of ammonium fulvate, 5 parts of boric acid and 60 parts of water; the effective viable count of the bacillus subtilis in the fermentation liquid is 350 hundred million/ml.
The preparation method of the compound microbial fertilizer comprises the following steps:
(1) activating bacillus subtilis (ACCC10242) by a slant culture medium, transferring the activated bacillus subtilis (ACCC10242) into a liquid seed culture medium, performing shake culture to obtain a liquid seed A, inoculating the prepared liquid seed A into a liquid fermentation culture medium, regulating the pH value to 7.5, regulating the aeration ratio to be 1:1, rotating speed to be 200 rpm, culturing at 37 ℃ for 38 hours to obtain a fermentation liquid, wherein the effective viable count of the bacillus subtilis in the fermentation liquid is 350 hundred million/ml;
the slant culture conditions in the step (1) are as follows: culturing in slant culture medium at 37 deg.C for 48 hr; the slant culture medium comprises the following components per liter:
9g of peptone, 5g of yeast powder, 4g of beef extract, 5g of sodium chloride, 20g of agar, 1g of monopotassium phosphate, 1g of dipotassium phosphate and the balance of water;
the shake flask culture conditions in the step (1) are as follows: culturing at 37 deg.C for 48 h; the liquid seed culture medium comprises the following components per liter:
9g of peptone, 8g of glucose, 5g of yeast powder, 4g of beef extract, 5g of sodium chloride, 1g of monopotassium phosphate, 1g of dipotassium phosphate and the balance of water;
the liquid fermentation medium in the step (1) comprises the following components per liter:
15g of corn flour, 15g of bean cake powder, 10g of glucose, 8g of ammonium sulfate, 5g of yeast powder, 0.8g of monopotassium phosphate, 0.25g of magnesium sulfate, 0.25g of manganese sulfate, 0.2g of ferrous chloride and the balance of water, and the pH value is adjusted to be 7.8.
(2) And (2) putting the bacillus subtilis fermentation liquor prepared in the step (1), sugar alcohol calcium, monopotassium phosphate, ammonium fulvate, boric acid and water in the mixing tank according to the weight parts, and uniformly stirring for 40 minutes.
(3) And (3) feeding the compound microbial fertilizer prepared in the step (2) into a vibrating screen of 150 meshes for screening, and removing particulate impurities larger than 150 meshes, so that drip irrigation application is facilitated, and a drip irrigation pipe head is prevented from being blocked.
(4) And (4) packaging the compound microbial fertilizer screened in the step (3).
Comparative example 1
The difference from the embodiment 3 is that the composite microbial fertilizer is not added with a bacillus subtilis (ACCC10242) fermentation liquid, but is added with a bacillus subtilis (LZH-H1) fermentation liquid which is prepared by the same method and has the same effective viable count.
Comparative example 2
The difference from the embodiment 3 is that the composite microbial fertilizer does not add bacillus subtilis (ACCC10242)) fermentation liquor, but adds bacillus licheniformis (LZH-13) which is prepared by the same method and has the same effective viable count.
Comparative example 3
The difference from the example 3 is that the compound microbial fertilizer is not added with the fermentation liquor of bacillus subtilis (ACCC10242), and the rest is the same.
Comparative example 4
The difference from the embodiment 3 is that the compound microbial fertilizer does not add ammonium fulvate, and the rest is the same.
Comparative example 5
The difference from the embodiment 3 is that the compound microbial fertilizer is not added with borax, and the rest is the same.
Comparative example 6
The difference from the embodiment 3 is that the compound microbial fertilizer does not contain sugar alcohol calcium and is replaced by calcium nitrate, and the rest is the same.
Comparative example 7
The difference from the embodiment 3 is that the compound microbial fertilizer is not added with potassium dihydrogen phosphate, and the rest is the same.
Examples of effects
The effect of the composite microbial fertilizer prepared in examples 1 to 3 and comparative examples 1 to 7 was verified in the town of zang house of Xixia city, cigarette platform, Shandong province.
Selecting red Fuji apples with basically consistent tree shape and growth vigor and tree age of 10 years as application objects, and performing effect verification comparison; the use method comprises the following steps: the compound microbial fertilizer prepared in the examples 1-3 and the comparative examples 1-7 is applied once by root irrigation and once by root irrigation in the leaf-expanding period of apple trees, once by root irrigation after flower falling and once by foliar spraying 40 days before harvesting, the correspondingly prepared fertilizer is used as an experimental treatment group, 10 treatment groups in total, and clear water is used as a blank control as a treatment group; each treatment group is provided with 5 parallel trees, each treatment group is provided with 3 trees, when the apples are to be harvested, 10 apples are harvested at random for statistical analysis of the number of the bitter pox lesions, grading is carried out according to the following table 1 to distinguish the disease degree, the average value of the 3 trees is the representative value of the parallel trees, and the disease degree index of each treatment group is shown in the table 2.
According to the determination of the calcium content in GB5009.92-2006 food, 5 apples are randomly selected in parallel for each treatment group, 11 apples with the same size and color are selected for calcium content detection, the detection parts are consistent, and the detection results of the calcium content of the apples are shown in Table 3.
The method comprises the steps of measuring the total number of bacteria (using a nutrient broth agar culture medium) and the total number of fungi (using a PDA culture medium) in a soil sample according to a measuring method of 5.1 effective viable bacteria number in NY/T2321-2013 microbial fertilizer product inspection regulations, randomly selecting 2 trees in parallel for each treatment group, selecting 4 points at the outer edge of a crown of each tree and 4 points at two thirds of the trunk from the outer edge of the crown, collecting 1kg of soil sample with the depth of 15cm from 8 points in total, finally obtaining 0.5kg of sample for detection by using a quartering method, wherein the sampling method before application is that 1 tree is randomly selected from each group of all treatment groups, sampling 8 points for 11 trees in total according to the same method, and finally obtaining 0.5kg of sample for detection by using the quartering method.
TABLE 1
| Grade | Number of lesions |
| 0 | 0 |
| 1 | 1-5 |
| 2 | 6-10 |
| 3 | 11-15 |
| 4 | 16-20 |
| 5 | ≥21 |
Disease severity index: Σ (number of diseased fruits × corresponding rank)/(number of diseased fruits × 5) × 100
TABLE 2
TABLE 3 calcium content of apple (mg/kg)
TABLE 4 changes in the indexes related to the soil flora
The results show that the composite microbial fertilizer has an outstanding comprehensive treatment effect, effectively reduces the disease index of apple pockmark disease, has an obvious effect on improving the calcium content of apples, has an obvious effect on improving the total number of soil floras, improves the quality of apples, reduces the disease degree and realizes good economic benefit.
Claims (6)
1. The compound microbial fertilizer for preventing and treating apple pockmark disease is characterized by comprising the following components in parts by weight:
1-5 parts of Bacillus subtilis fermentation liquor, 20-30 parts of sugar alcohol calcium, 5 parts of monopotassium phosphate, 15-30 parts of ammonium fulvate, 5 parts of boric acid and 40-60 parts of water;
the effective viable count of the bacillus subtilis in the fermentation liquor is more than or equal to 250 hundred million/ml.
2. The compound microbial fertilizer according to claim 1, which comprises the following components in parts by weight: 5 parts of bacillus subtilis fermentation liquor, 30 parts of sugar alcohol calcium, 5 parts of monopotassium phosphate, 30 parts of ammonium fulvate, 5 parts of boric acid and 60 parts of water;
the effective viable count of the bacillus subtilis in the fermentation liquid is 350 hundred million/ml.
3. The compound microbial fertilizer according to claim 1 or 2, wherein the bacillus subtilis strain number in the bacillus subtilis fermentation broth is ACCC 10242.
4. The preparation method of the compound microbial fertilizer as claimed in claim 1, which is characterized by comprising the following steps:
(1) activating bacillus subtilis by a slant culture medium, transferring the activated bacillus subtilis into a liquid seed culture medium, performing shake culture to obtain a liquid seed A, inoculating the prepared liquid seed A into a liquid fermentation culture medium, regulating the pH value to 7.0-8.0, regulating the aeration ratio to be 1 (0.5-1) and the rotation speed to be 100-200 rpm, and culturing at 30-37 ℃ for 36-48 hours to obtain a fermentation liquid, wherein the effective viable count of the bacillus subtilis in the fermentation liquid is more than or equal to 250 hundred million/ml;
the slant culture conditions in the step (1) are as follows: culturing in a slant culture medium at the temperature of 30-37 ℃ for 24-48 h; the slant culture medium comprises the following components per liter:
6-9 g of peptone, 3-5 g of yeast powder, 2-4 g of beef extract, 3-5 g of sodium chloride, 15-25 g of agar, 0.5-1 g of monopotassium phosphate, 0.5-1 g of dipotassium phosphate and the balance of water;
the shake flask culture conditions in the step (1) are as follows: culturing for 36-48 h at 30-37 ℃; the liquid seed culture medium comprises the following components per liter:
6-9 g of peptone, 5-8 g of glucose, 3-5 g of yeast powder, 2-4 g of beef extract, 3-5 g of sodium chloride, 0.5-1 g of monopotassium phosphate, 0.5-1 g of dipotassium phosphate and the balance of water;
the liquid fermentation medium in the step (1) comprises the following components per liter:
10-15 g of corn flour, 10-15 g of bean cake powder, 5-10 g of glucose, 5-10 g of ammonium sulfate, 3-5 g of yeast powder, 0.5-0.8 g of monopotassium phosphate, 0.1-0.25 g of magnesium sulfate, 0.1-0.25 g of manganese sulfate, 0.1-0.2 g of ferrous chloride and the balance of water, and adjusting the pH value to 7-8;
(2) uniformly mixing the bacillus subtilis fermentation broth prepared in the step (1), sugar alcohol calcium, monopotassium phosphate, ammonium fulvate, boric acid and water in parts by weight to prepare a composite microbial fertilizer;
(3) and (3) performing 150-mesh screening on the composite microbial fertilizer prepared in the step (2), removing particulate impurities larger than 150 meshes, facilitating drip irrigation application and avoiding blocking a drip irrigation pipe head.
5. The method for preparing the compound microbial fertilizer as claimed in claim 4, wherein the effective viable count of the fermentation liquor prepared in the step (1) is 350 hundred million/ml; and (2) uniformly mixing 5 parts by weight of the bacillus subtilis fermentation liquid prepared in the step (1), 30 parts by weight of sugar alcohol calcium, 5 parts by weight of monopotassium phosphate, 30 parts by weight of ammonium fulvate, 5 parts by weight of boric acid and 60 parts by weight of water to prepare the composite microbial fertilizer.
6. The compound microbial fertilizer as claimed in any one of claims 1, 4 and 5 is applied to control of apple bitter pox.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911335501.4A CN111072427B (en) | 2019-12-20 | 2019-12-20 | Composite microbial fertilizer for preventing and treating apple pockmark disease and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911335501.4A CN111072427B (en) | 2019-12-20 | 2019-12-20 | Composite microbial fertilizer for preventing and treating apple pockmark disease and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111072427A true CN111072427A (en) | 2020-04-28 |
| CN111072427B CN111072427B (en) | 2022-03-22 |
Family
ID=70316840
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911335501.4A Active CN111072427B (en) | 2019-12-20 | 2019-12-20 | Composite microbial fertilizer for preventing and treating apple pockmark disease and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111072427B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111919651A (en) * | 2020-08-05 | 2020-11-13 | 西北农林科技大学 | Method for supplementing calcium to honey-crisp apple fruits |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105732224A (en) * | 2016-02-21 | 2016-07-06 | 宿林功 | Bioorganic fertilizer for effectively preventing bitter pit disease of apples |
| CN106495951A (en) * | 2016-10-27 | 2017-03-15 | 德州市元和农业科技开发有限责任公司 | A kind of high concentration microorganism microbial inoculum containing polyglutamic acid and preparation method and application |
| CN109608273A (en) * | 2019-02-15 | 2019-04-12 | 王刚 | Long-acting organic mineralization activity fertilizer of one kind and preparation method thereof |
| CN109704884A (en) * | 2019-02-15 | 2019-05-03 | 王刚 | Long-acting organic mineralization activity foliar fertilizer of one kind and preparation method thereof |
-
2019
- 2019-12-20 CN CN201911335501.4A patent/CN111072427B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105732224A (en) * | 2016-02-21 | 2016-07-06 | 宿林功 | Bioorganic fertilizer for effectively preventing bitter pit disease of apples |
| CN106495951A (en) * | 2016-10-27 | 2017-03-15 | 德州市元和农业科技开发有限责任公司 | A kind of high concentration microorganism microbial inoculum containing polyglutamic acid and preparation method and application |
| CN109608273A (en) * | 2019-02-15 | 2019-04-12 | 王刚 | Long-acting organic mineralization activity fertilizer of one kind and preparation method thereof |
| CN109704884A (en) * | 2019-02-15 | 2019-05-03 | 王刚 | Long-acting organic mineralization activity foliar fertilizer of one kind and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 孙瑞红等: "《果树病虫害安全防治》", 31 January 2018, 中国科学技术出版社 * |
| 王芳等: "钙抑制植物病害作用及机制的研究进展", 《生物技术通报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111919651A (en) * | 2020-08-05 | 2020-11-13 | 西北农林科技大学 | Method for supplementing calcium to honey-crisp apple fruits |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111072427B (en) | 2022-03-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106187515B (en) | Utilize the hickory chick nutrient bag and preparation method thereof of edible fungi residue production | |
| CN110810174B (en) | Application of trichoderma harzianum LTR-2 in cereal crop cultivation | |
| CN107580818B (en) | A kind of integrated approach of soil conditioning and reparation | |
| CN110627537A (en) | Multifunctional microbial fertilizer and preparation method thereof | |
| CN112358345A (en) | Biological seaweed fertilizer, bacterial liquid for biological fertilizer and preparation method of bacterial liquid | |
| CN114586890A (en) | Method for preparing composite flora feed by utilizing juncao | |
| CN113072414A (en) | Application of holotrichoderma bioorganic fertilizer | |
| CN111320507A (en) | Functional liquid fertilizer, preparation method thereof and cotton fertilization method | |
| CN111072427B (en) | Composite microbial fertilizer for preventing and treating apple pockmark disease and preparation method thereof | |
| CN117736944A (en) | Streptomyces griseus as well as microbial inoculum and application thereof | |
| CN106367359A (en) | Aspergillus niger and application thereof to preparing citric acid from fermented acorns | |
| CN111377768A (en) | Special tobacco organic fertilizer with functions of disease resistance, quality improvement and development promotion | |
| JP2012228242A (en) | Method for cultivating polygonaceae crop | |
| CN115043678A (en) | Biogas residue solid-state fermentation bio-organic fertilizer and preparation method and application thereof | |
| CN111592405B (en) | Organic water-soluble fertilizer capable of resisting wheat dry hot air and powdery mildew as well as preparation and application thereof | |
| CN108085226A (en) | A kind of production method of organic health fruit wine | |
| CN107176865A (en) | The sweetened liquid fertilizer method of preparation and use of marine alga earthworm hydrolyzate efficient bio-active | |
| CN107827653B (en) | High-concentration organic carbonic acid fermentation liquor and preparation method and application thereof | |
| CN112457077A (en) | Fungus solid fermentation organic fertilizer and preparation method thereof | |
| KR20220057782A (en) | Method for producing organic fertilizer having activity of plant disease control using spent mushroom substrate | |
| CN111995477A (en) | Continuous cropping resistant bio-organic fertilizer and preparation method thereof | |
| CN111606742A (en) | Special biological source bacterial fertilizer system for winter jujubes and use method thereof | |
| CN113527001A (en) | Lees treatment method, foliar fertilizer, organic fertilizer and preparation method thereof | |
| KR20100019015A (en) | Organic fertilizer containing stevia | |
| CN112062645B (en) | Fertilizer composition for reducing cherry fruit cracking rate and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 253000 no.729, Hongyin Road, Dezhou Economic Development Zone, Dezhou City, Shandong Province Applicant after: Yuanhe Biotechnology (Dezhou) Co.,Ltd. Address before: 253000 no.729, Hongyin Road, Dezhou Economic Development Zone, Dezhou City, Shandong Province Applicant before: DEZHOU YUANHE AGRICULTURAL TECHNOLOGY DEVELOPMENT Co.,Ltd. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |