CN117736944A - Streptomyces griseus as well as microbial inoculum and application thereof - Google Patents
Streptomyces griseus as well as microbial inoculum and application thereof Download PDFInfo
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- CN117736944A CN117736944A CN202410187527.3A CN202410187527A CN117736944A CN 117736944 A CN117736944 A CN 117736944A CN 202410187527 A CN202410187527 A CN 202410187527A CN 117736944 A CN117736944 A CN 117736944A
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a Streptomyces griseus strain, a microbial inoculum and application thereof, wherein the preservation number of the Streptomyces griseus strain is as follows: cctccc No. m 20231643; the streptomyces griseowensis YNK-FS0018 and the microbial inoculum provided by the invention have control effects on pepper blight caused by phytophthora capsici, star aniseed root rot caused by fusarium oxysporum, tea tree southern blight caused by sclerotium rolfsii and tsaoko root rot caused by sclerotium rolfsii; the mycotic agent prepared by adding algal polysaccharide into the streptomyces griseus of the invention can further improve the control effect of the crop diseases.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to streptomyces griseoviridis, a microbial inoculum and application thereof.
Background
Along with rapid development of economic planting, the following crop diseases are increasingly prominent, and the continuous healthy development of industry is seriously affected. Especially, once the rhizome diseases occur in the field, the rhizome diseases face large-area harvest, and irrecoverable losses are caused to farmers.
Commercial crops such as pepper, star anise, tea tree, tsaoko and the like are economic sources of traditional Chinese medicines for peasants in mountain areas of Yunnan province, and are also the prop industry in partial areas. At present, in the planting system of the crops, the planting of the crops excessively depends on chemical fertilizers and chemical pesticides, and a production mode of continuous cropping of single crops is adopted, and soil pollution and other nutrient elements are caused by long-term high-volume fertilizer application and unbalanced fertilizationUnbalanced, partial element eutrophication and soil acidification aggravate, so that the physical and chemical properties of the soil are worsened, the microbial community structure is unbalanced, the soil quality is finally degraded, the resistance of crops is reduced, and root disease outbreaks, especially root rot outbreaks of crops, such as phytophthora capsici, are causedPhytophthora capsici) Caused pepper blast and fusarium oxysporumFusarium oxysporum) Leading to the star anise root rot and the sclerotium rolfsiiSclerotium rolfsiiSacc.) caused southern blight of tea and sclerotinia sclerotiorumScleritinia sclerotioum) Resulting in tsaoko root rot.
In the prior art, the control method for the root rot of the crops is single, chemical agents are still used in a large area, and the use of some chemical agents can further damage the environment. At present, no sustainable and effective method for preventing and controlling root rot of crops is available, economical and environment-friendly.
In addition, although the prior art has reported the prevention and treatment of root rot using microbial agents, there is still room for further improvement in the prevention and treatment effects of existing microbial agents. Therefore, there is a need to develop a new, green, economical product capable of continuously and effectively preventing and controlling root rot of crops, and a method for realizing industrial sustainable development.
Disclosure of Invention
In order to overcome the problems in the prior art, the invention provides the streptomyces griseus and the microbial inoculum and application thereof, which can effectively improve the control effect on crop rhizome diseases, reduce the content of plant rhizosphere phenolic acid compounds and reduce the chemical effect of crops; the proline content and the activity of the plant root system are improved; improving the activity of the plant root system defensive enzyme; thereby regulating the microecology of the soil and improving the stress resistance of the plants. In addition, the invention also provides a mixed application of the seaweed polysaccharide and the streptomyces griseus preparation, and the seaweed polysaccharide is used as a component in the microbial inoculum by utilizing the functions of bacteriostasis, antioxidation and the like of the seaweed polysaccharide, so that the disease control effect of the microbial inoculum can be improved, the bacteriostasis activity of the microbial inoculum is improved, meanwhile, the streptomyces griseus preparation can not generate side effects, a certain protection effect can be realized on the streptomyces griseus, the storage loss rate of the streptomyces griseus is reduced, the shelf life of the streptomyces griseus microbial inoculum is prolonged, and meanwhile, the control effect on crop root rot can be effectively improved, the soil microecology is regulated, and the plant stress resistance is improved.
In order to achieve the above purpose, the invention is realized by the following technical scheme:
the invention provides a microbial inoculum, which contains the following preservation numbers: streptomyces oryzae of CCTCC NO. M20231643 and algal polysaccharide; the microbial inoculum is used for improving the activity of plant defensive enzymes, and/or improving the proline content and the root activity of plant roots, and/or preventing and treating crop diseases.
The present invention provides a method of promoting crop growth and/or controlling crop soil-borne diseases, the method comprising: the microbial inoculum is applied to soil, and the total dosage of the streptomyces griseus is not less than 2 multiplied by 10 10 cfu/strain/time.
Through the technical scheme, the invention at least has the following beneficial effects:
(1) When the microbial inoculum is applied to soil, the content of plant rhizosphere phenolic acid compounds can be reduced, and the principle is that the streptomyces griseus can take phenolic acid substances secreted by plants in the soil as carbon sources, so that the content of phenolic acid in the soil is reduced, the acidification of the soil is inhibited, and a soil environment favorable for inhibiting the occurrence of diseases is formed.
(2) The streptomyces griseus YNK-FS0018 is plant rhizosphere bacteria separated from tobacco plants, and has better soil and plant rhizosphere colonization capability compared with a plurality of artificially cultured strains in the prior art in a genetic engineering or mutation induction mode, and is more beneficial to continuously playing roles after application.
(3) The microbial inoculum provided by the invention can effectively improve soil fertility, improve soil quality, and is beneficial to the absorption and utilization of nutrients by crops, thereby improving crop yield and quality. The method comprises the following steps: the microbial inoculum can improve the proline content, the activity and the defensive enzyme activity of the root system of crops, and solves the problems that the existing chemical fertilizer has poor effect of promoting the growth of crops or can not realize continuous promotion of the growth.
(4) The seaweed polysaccharide and the streptomyces griseus preparation are mixed and applied, and the seaweed polysaccharide is used as one component in the microbial inoculum by utilizing the functions of bacteriostasis, antioxidation and the like of the seaweed polysaccharide, so that the control effect and the bacteriostasis activity of the microbial inoculum on diseases can be improved, the streptomyces griseus preparation can not be subjected to side effects, the streptomyces griseus can be protected to a certain extent, the storage loss rate of the streptomyces griseus is reduced, the shelf life of the streptomyces griseus microbial inoculum is prolonged, and meanwhile, the control effect on crop root rot can be effectively improved, the soil microecology can be regulated, and the stress resistance of plants can be improved.
(5) The microbial inoculum provided by the invention takes algal polysaccharide (waste) as a main raw material, and has the characteristics of wide raw material source, low cost, green and environment-friendly performance and simple preparation method. Meanwhile, the production and application of the microbial inoculum can promote the recycling of wastes and the sustainable development of agricultural production, is beneficial to promoting the adjustment of an industrial structure, and has positive significance for the improvement of the total agricultural yield and the income increase of farmers.
Preservation of organisms
The invention provides streptomyces griseoviridae（Stretomyces blastmyceticus）Classified and named asStretomyces blastmyceticusYNK-FS0018 was deposited in China center for type culture collection (China) on 9 th month 7 th year, and has an address of 299 th No. eight Wuchang district of Wuhan, hubei province, and a deposit number of CCTCC NO: m20231643.
Detailed Description
Materials and reagents used in the present invention are commercially available unless otherwise indicated.
The endpoints and any values of the ranges disclosed herein are not limited to the precise range or value, and are understood to encompass values approaching those ranges or values. For numerical ranges, one or more new numerical ranges may be found between the endpoints of each range, between the endpoint of each range and the individual point value, and between the individual point value, in combination with each other, and are to be considered as specifically disclosed herein.
In the invention, the streptomyces blasticidin YNK-FS0018 and streptomyces blasticidin CCTCC NO. M20231643 are the same strain, the meanings of the two are the same, and the names (numbers) of the two are used interchangeably.
The invention relates to a streptomyces griseusStretomyces blastmyceticus) YNK-FS0018, published in journal microbiology, treatise subject name: screening and identification of three phenolic acid degrading bacteria and its biological activity (https:// doi.org/10.13344/j.
The inventor finds that the Streptomyces griseus itself or the Streptomyces griseus is prepared into a microbial inoculum in the research, and the microbial inoculum is applied to the soil, so that the activity of plant defense enzymes can be effectively improved, the proline content and the root activity of plant roots can be improved, the phenolic acid content of plant rhizosphere soil can be reduced, and the use amount of chemical fertilizers can be reduced. In addition, the application of the streptomyces oryzae itself or the microbial inoculum can promote plant growth and increase yield. Through further research, the inventor also discovers that the streptomyces griseus itself or the microbial inoculum is effective to the phytophthora capsiciPhytophthora capsici) Caused pepper blast and fusarium oxysporumFusarium oxysporum) Leading to the star anise root rot and the sclerotium rolfsiiSclerotium rolfsiiSacc.) caused southern blight of tea and sclerotinia sclerotiorumScleritinia sclerotioum) The resulting tsaoko root rot has better control effect.
The inventor also discovers in the research that the mycotic agent prepared by mixing seaweed polysaccharide and streptomyces griseus in a certain proportion can effectively reduce the loss rate of the streptomyces griseus, can effectively improve the activity of plant defensive enzymes when being applied to soil, improves the proline content and the root activity of plant roots, reduces the phenolic acid content of plant rhizosphere soil and reduces the use amount of chemical fertilizers. In addition, the application of the microbial inoculum can promote plant growth and improve yield. Through further research, the inventor also discovers that the microbial inoculum is effective for phytophthora capsiciPhytophthora capsici) Caused pepper blast and fusarium oxysporumFusarium oxysporum) Leading to the star anise root rot and the sclerotium rolfsiiSclerotium rolfsiiSacc.) caused southern blight of tea and sclerotinia sclerotiorumScleritinia sclerotioum) The resulting tsaoko root rot has better control effect.
Based on the above, the invention provides a microbial inoculum, which contains the following preservation numbers: streptomyces oryzae of CCTCC NO. M20231643 and algal polysaccharide; the microbial inoculum is used for improving the activity of plant defensive enzymes, and/or improving the proline content and the root activity of plant roots, and/or preventing and treating crop diseases.
Preferably, the storage number of the microbial inoculum is: the weight ratio of the streptomyces griseus of CCTCC No. M20231643 to the seaweed polysaccharide is 18:1-3.
Preferably, the content of the streptomyces griseoviridis in the microbial inoculum is not less than 1×10 6 cfu/g (solid microbial agent) or 1X 10 6 cfu/mL (liquid or concentrated). For example, it may be 2X 10 6 -3×10 8 cfu/g or 2X 10 6 -3×10 8 cfu/mL。
In the present invention, the raw material (e.g., algal polysaccharide) used as the above-mentioned microbial inoculum is not particularly limited, and may be any related product which can be used in the art for preparing microbial inoculum, either commercially available related products or related products which are prepared by themselves according to the prior art.
According to a preferred embodiment of the present invention, wherein the algal polysaccharide has a moisture content of 10 to 20 wt%;
preferably, the algal polysaccharide has an L-fucose content of 20-30 wt%, a D-xylose content of 15-25 wt%, a D-hexenuronic acid content of 10-20 wt%, a sulfate content of 8-15 wt%, and a protein content of 7-12% on a dry matter basis.
Preferably, the amount of the streptomyces grignard in the microbial inoculum is such that the streptomyces grignard in the microbial inoculum is 1×10 6 -3×10 8 cfu/g。
Wherein the crop diseases are pepper blast, star anise root rot, tea tree southern blight and tsaoko root rot.
In the present invention, the improvement of the activity of plant defensive enzyme means the improvement of superoxide dismutase (SOD) (Ug) of plant root system -1 ) Polyphenol Oxidase (PPO) (Ug) -1 ) Activity, reducing plant rhizosphere soil phenolic acid (e.g.: benzoic acid) content, improving stress resistance of plants means that the proline content of plant root systems is improved, the activity of the plant root systems is improved, and the like.
In the present invention, controlling plant diseases means preventing or reducing occurrence of plant diseases, or reducing loss caused by diseases after occurrence of diseases, and the like.
The invention also provides a method for promoting plant growth and/or controlling plant diseases, which comprises applying the microbial inoculum to soil.
Specific features of the microbial inoculum employed in the method provided by the invention are as described above and will not be described in detail herein.
In the present invention, the specific amount of the microbial inoculum is not particularly limited as long as it can promote plant growth.
According to a preferred embodiment of the present invention, the microbial inoculum is used in an amount such that Streptomyces griseus is applied to the soil in an amount of not less than 2X 10 8 cfu/strain/time, preferably may be 1X 10 9 -5×10 12 cfu/strain/time. For example, it may be 1X 10 9 cfu/strain/time, 2X 10 9 cfu/strain/time, 3×10 9 cfu/strain/time, 5X 10 9 cfu/strain/time, 8×10 9 cfu/strain/time, 1×10 10 cfu/strain/time, 3×10 10 cfu/strain/time, 5X 10 10 cfu/strain/time, 8×10 10 cfu/strain/time, 1×10 11 cfu/strain/time, 3×10 11 cfu/strain/time, 5X 10 11 cfu/strain/time, 8×10 11 cfu/strain/time, 1×10 12 cfu/strain/time, 2X 10 12 cfu/strain/time, 3×10 12 cfu/strain/time, 4X 10 12 cfu/strain/time, 5X 10 12 cfu/strain/time, or any intermediate value between any two values mentioned above. The dosage of the streptomyces griseoviridis is calculated according to the application dosage of the microbial inoculum and the quantity of the streptomyces griseoviridis contained in the microbial inoculum, wherein the liquid microbial inoculum is converted according to 1 g/mL.
According to a preferred embodiment of the present invention, the microbial agent may be used in the soil in an amount of 300 to 500 g/plant/time.
Preferably, the frequency of administration of the bacterial agent may be once every 3 months.
In order to make the objects, technical solutions and advantageous effects of the present invention more apparent, preferred embodiments of the present invention will be described in detail below to facilitate understanding by the skilled person.
Example one cultivation of Streptomyces griseus and preparation of a microbial inoculum:
(1) Performing solid culture on the streptomyces griseus in a solid culture medium to obtain test tube species;
(2) Preparing a liquid seed culture medium, and inoculating test tube seeds into the liquid seed culture medium for liquid culture to obtain liquid seeds;
(3) Preparing a liquid fermentation medium, and inoculating liquid seeds into the liquid fermentation medium for fermentation.
In the step (1), the streptomyces griseus is inoculated by adopting a slant inoculation mode, and the solid culture is carried out at the temperature of 25-30 ℃ for 3-5d; the solid medium comprises: 4-6g/L yeast extract powder, 10-15g/L malt extract powder, 4-6g/L glucose, 15-20g/L agar, and pH=6.5-7.5.
In the step (2), inoculating test tube seeds into a liquid seed culture medium, and treating the liquid seed culture medium for 3-5d under the conditions that the temperature is 25-30 ℃ and the rotating speed is 180-220 r/min; the liquid seed medium comprises: 4-6g/L yeast extract powder, 10-15g/L malt extract powder, 4-6g/L glucose and pH=6.5-7.5.
In this step, the liquid seed culture medium is sterilized at 120-125deg.C for 20-30min, cooled, and then added into 100mL liquid seed culture medium at 0.5-1.5cm 2 Culturing in a shaker at 28-30deg.C at 180-220r/min for 3-5d to obtain liquid seed.
In the step (3), inoculating liquid seeds in a liquid fermentation culture medium according to the inoculum size of 0.05-0.1:1 by volume ratio, and treating for 3-5d under the conditions of 25-30 ℃ and 180-220r/min of rotating speed; the liquid fermentation medium comprises: 4-6g/L yeast extract powder, 10-15g/L malt extract powder, 4-6g/L glucose and pH=6.5-7.5.
In this step, the liquid fermentation medium is sterilized preferably at 120-125℃for 20-30min, cooled, inoculated with liquid seeds, and cultured preferably at 28-30℃on a shaker at a rotation speed of 180-220r/min for 3-5d to obtain a fermentation broth.
The preparation steps further comprise:
diluting the obtained culture solution with fresh ISP2 culture medium to obtain viable bacteria number of about 2-3×10 8 cfu/mL of Streptomyces griseus liquid bacteria. Drying the liquid microbial inoculum to obtain dry powder bacteria with viable count of about 2-3×10 8 cfu/g。
Taking the streptomyces griseus dry powder (content 2×10) in the step (4) 8 cfu/g) 18 parts by weight was uniformly mixed with 1 part by weight of algal polysaccharide (purchased from Hebei Color Duotung Biotechnology Co., ltd.) powder to prepare a Streptomyces griseus microbial agent.
Example two effects of algal polysaccharide on Streptomyces griseus YNK-FS 0018:
(1) Taking Streptomyces griseus YNK-FS0018 dry powder (content 2×10) 8 cfu/g) 18 parts by weight and 1 part by weight of algal polysaccharide powder are uniformly mixed, and the mixture is stored in a vacuum sealing bag at normal temperature. After 3 months, the amount of Streptomyces griseus YNK-FS0018 was 1.723×10 8 cfu/g, mortality was 9.1%. The mortality rate of Streptomyces oryzae YNK-FS0018 dry powder alone after 3 months storage was 25.2%.
(2) Taking Streptomyces griseus YNK-FS0018 dry powder (content 2×10) 8 cfu/g) 18 parts by weight and 2 parts by weight of algal polysaccharide powder are uniformly mixed, and the mixture is stored in a vacuum sealing bag at normal temperature. After 3 months, the amount of Streptomyces griseus in the sample was 1.588×10 8 cfu/g, mortality was 16.2%.
(3) Taking Streptomyces griseus YNK-FS0018 dry powder (content 2×10) 8 cfu/g) 18 parts by weight and 3 parts by weight of algal polysaccharide powder are uniformly mixed, and the mixture is stored in a vacuum sealing bag at normal temperature. After 3 months, the amount of Streptomyces griseus in the sample was 1.515×10 8 cfu/g, mortality rate was 20.1%.
In conclusion, the seaweed polysaccharide and the streptomyces griseus are compounded into the microbial inoculum, so that the normal-temperature preservation death rate and the storage loss rate of the streptomyces griseus can be reduced, and the shelf life of the microbial inoculum can be prolonged.
Example Streptomyces griseus and application of microbial inoculum in preventing and controlling crop root diseases, regulating soil nutrient content and promoting growth
Experiment design:
selecting a batch of plants with basically consistent initial heights, and performing other field management with conventional production except for setting different levels of pesticide application. The test was started in spring with 5 treatments, 3 replicates for 15 cells. Randomly arranged, and high protection rows around. And the disease control effect (table 1), the influence on the activity of the plant root system defense enzymes (table 2), the influence on the stress resistance of the plant root system (table 3) and the degradation of plant rhizosphere soil phenolic acid (table 4) were investigated according to the experimental group I, the experimental group II, the experimental group III, the experimental group IV and the test method.
The disease investigation method comprises the following steps:
level 0: asymptomatic condition
Stage 1: the root of the plant has a small amount of disease spots of 0-10%.
3 stages: part of the lesion spots appear in the root 10-25%.
5 stages: root is completely infected.
7 stages: the plants die.
The morbidity and index were calculated.
Incidence = number of diseased plants/total number of investigated plants 100
Disease index = (Σ (number of disease stages of each stage of disease strain)/investigation of total number of plants of highest stage) 100
Determination of the content of phenolic acid (benzoic acid) in the rhizosphere soil of the crop plants:
(1) Benzoic acid standard configuration
0.1g of benzoic acid (analytically pure) is accurately taken and dissolved in purified water, and the volume is fixed to 1L by using a volumetric flask for measurement.
(2) Plant rhizosphere soil sample solution configuration
Respectively weighing each plant rhizosphere soil sample of 25. 25 g, placing the plant rhizosphere soil samples in 500mL conical flasks, respectively adding 200mL of 1mol/L NaOH solution, oscillating, performing ultrasonic treatment for 30min, and placing the plant rhizosphere soil samples on a shaking table for shaking for 2h. Filtering, adjusting pH of the filtrate to 2.5 with hydrochloric acid, standing overnight, and centrifuging. The supernatant was transferred to a 500mL separatory funnel, 200mL ethyl acetate solution was added to the mixture twice, and the organic phase was collected. Recovering ethyl acetate, dissolving the residue with methanol, fixing volume to 5mL, and filtering with 0.45um microporous membrane to obtain sample solution.
(3) Sample measurement
And respectively taking a benzoic acid standard substance and a plant rhizosphere soil sample solution, filtering, and detecting the substrate amount by using HPLC (high performance liquid chromatography), thereby calculating the substrate degradation efficiency. Conditions of high performance liquid phase: taking a sample of the liquid to be detected, adding the sample into a 50mL centrifuge tube, centrifuging for 10min at 8000r/min at 4 ℃, taking a supernatant, taking 1mL of the centrifuged fermentation liquor, and taking the fermentation liquor as a sample, wherein the sample is taken by a 0.22 mu m microporous filter membrane, and a chromatographic column is an Agilent C18 column (250 mm multiplied by 4.0mm multiplied by 5 mu m); the column temperature is 35 ℃; the ultraviolet detection wavelength is 230nm; the mobile phase is methanol: 0.02mol/L ammonium acetate = 5:95; the flow rate is 1.0mL/min; the sample volume was 10. Mu.L, eluted isocratically, peaks were detected and retention time was determined. The benzoic acid standard was determined to have a peak area of 4335.28369 and a retention time of 3.495min.
The method for measuring the activity of the plant root system defensive enzyme comprises the following steps:
superoxide dismutase (SOD) (U g) -1 ) Polyphenol Oxidase (PPO) (U g) -1 ) All assays were performed using kits purchased from suzhou grissin biotechnology limited, methods of use reference.
Determination of proline content of plant root system (μg/g) and viability of plant root system (mg/g/h):
all assays were performed using kits purchased from suzhou grissin biotechnology limited, methods of use reference.
Experimental group I: streptomyces griseus culture medium (the viable count is about 2×10) 8 cfu/mL), 300 mL/plant/time, drenching to plant rhizosphere (50 plants per test group) 1 time every 90 days, investigating each disease control effect (Table 1) in 10-11 months of the current year, defending plant root systemThe effect of enzyme activity (table 2), on the stress resistance of the plant root system (table 3) and on the degradation of plant rhizosphere soil phenolic acid (table 4) were investigated.
Experimental group II: the Streptomyces griseus dry powder of example one (number of viable bacteria is about 2X 10) 8 cfu/g), 300 g/plant/time, applied to plant rhizosphere soil (50 plants per test group); the plants were watered 1 time at 90 days intervals, and the effects of controlling each disease (Table 1), the effects on the activity of the plant root defensive enzymes (Table 2), the effects on the stress resistance of the plant root (Table 3) and the degradation of the plant rhizosphere soil phenolic acid (Table 4) were investigated in 10-11 months of the current year.
Experimental group III: the Streptomyces griseus dry powder of example one (number of viable bacteria is about 2X 10) 8 cfu/g), 18 parts by weight and 1 part by weight of algal polysaccharide powder are uniformly mixed to obtain a streptomyces griseus microbial inoculum, 300 g/plant/time, and the streptomyces griseus microbial inoculum is applied to plant rhizosphere (50 plants in each test group); the plant root system stress-resistance test was carried out 1 time at 90 days, and the disease control effect (table 1), the effect on the plant root system defensive enzyme activity (table 2), the effect on the plant root system stress-resistance (table 3) and the degradation of plant rhizosphere soil phenolic acid (table 4) were investigated in 10-11 months of the current year.
Experiment group IV: the Streptomyces griseus dry powder of example one (number of viable bacteria is about 2X 10) 8 cfu/g) 18 parts by weight and 2 parts by weight of seaweed polysaccharide powder are uniformly mixed to obtain a streptomyces griseus microbial inoculum, 500 g/plant/time, and the streptomyces griseus microbial inoculum is applied to plant rhizosphere (50 plants in each test group); the plant root system stress-resistance test was carried out 1 time at 90 days, and the disease control effect (table 1), the effect on the plant root system defensive enzyme activity (table 2), the effect on the plant root system stress-resistance (table 3) and the degradation of plant rhizosphere soil phenolic acid (table 4) were investigated in 10-11 months of the current year.
Experimental group v: the Streptomyces griseus dry powder of example one (number of viable bacteria is about 2X 10) 8 cfu/g) 18 parts by weight and 3 parts by weight of seaweed polysaccharide powder are uniformly mixed to obtain a streptomyces griseus microbial inoculum, 500 g/plant/time, and the streptomyces griseus microbial inoculum is applied to plant rhizosphere (50 plants in each test group); applied 1 time at 90 days intervals, and the disease control effect (Table 1) and the activity of the plant root system defense enzyme were examined in 10-11 months of the current yearThe effect (table 2), the effect on the stress resistance of the plant root system (table 3) and the degradation of the plant rhizosphere soil phenolic acid (table 4) were investigated.
Blank control group: neither the culture solution nor the bacterial agent of the streptomyces griseopensis is applied.
Positive control group: and (3) fertilizing, namely self-preparing urea, calcium superphosphate and potassium sulfate, wherein the weight ratio of N, P, K is 17.5:8.1:25.
Table 1: streptomyces griseoviridis preparation with disease field control effect
From the data in table 1, it can be seen that the disease control effect of the test group of streptomyces griseus, the dry powder fungus and the microbial inoculum is significantly better than that of the blank control group and the positive control group, wherein the preparation effect of the test group iii is the best. Comparing the experimental groups III, IV and V with the experimental group I, the disease control effect is obviously improved after the seaweed polysaccharide is added into the streptomyces griseopestife.
Table 2: effect of Streptomyces Pyricularis preparation on plant root system defensive enzyme Activity
From the data in Table 2, it is clear that the regulation of the effect of Streptomyces griseus, dry powder bacteria and microbial inoculum of the experimental group on the plant root system defensive enzyme activity is significantly better than that of the blank control group and the positive control group, wherein the preparation effect of the experimental group III is optimal. Meanwhile, compared with the experimental groups III, IV and V, the experimental group I, after the seaweed polysaccharide is added into the streptomyces griseus, the regulation of the influence on the activity of the plant root defensin enzyme is obviously better than that of the single application of the streptomyces griseus.
Table 3: influence of Streptomyces Pyriculatus preparation on stress resistance of plant root system
From the data in Table 3, the effect of Streptomyces griseus, dry powder bacteria and microbial inoculum in the experimental group on improving the stress resistance of plant root system is obviously superior to that of the blank control group and the positive control group, wherein the preparation effect in the experimental group III is the best. Meanwhile, compared with the experimental groups III, IV and V, the effect of improving the stress resistance of plant roots is obviously better than that of single use of the streptomyces griseus after the seaweed polysaccharide is added into the streptomyces griseus.
Table 4 degradation of crop rhizosphere phenolic acids by streptomyces griseus formulations
From the data in table 4, the degradation effect of the streptomyces griseus, the dry powder bacteria and the microbial inoculum of the experimental group on plant rhizosphere soil phenolic acid is obviously better than that of the blank control group and the positive control group, wherein the preparation effect of the experimental group III is the best. Meanwhile, compared with the experimental groups III, IV and V, the degradation effect on plant rhizosphere soil phenolic acid is obviously better than that of the single use of the streptomyces oryzae after the seaweed polysaccharide is added into the streptomyces oryzae.
In summary, the invention provides the mixed application of the seaweed polysaccharide and the streptomyces griseus preparation, and the seaweed polysaccharide is used as one component in the microbial inoculum by utilizing the bacteriostasis and antioxidation functions of the seaweed polysaccharide, so that the disease control effect of the microbial inoculum can be improved, the bacteriostasis activity of the microbial inoculum is improved, meanwhile, the streptomyces griseus preparation cannot generate side effects, the streptomyces griseus has a certain protection effect, the storage loss rate of the streptomyces griseus is reduced, the shelf life of the streptomyces griseus microbial inoculum is prolonged, and meanwhile, the microbial inoculum has a profound significance in effectively improving the disease control effect, improving the plant stress resistance and constructing healthy soil microecology.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (7)
1. A microbial inoculum, characterized in that the microbial inoculum comprises the following preservation numbers: streptomyces griseus and algal polysaccharide of CCTCC No. M20231643, wherein the microbial inoculum is used for improving the activity of plant defensive enzymes, and/or improving the proline content and the root activity of plant roots, and/or preventing and treating crop diseases.
2. A microbial agent according to claim 1, wherein: the preservation number of the microbial inoculum is as follows: the weight ratio of the streptomyces griseus of CCTCC No. M20231643 to the seaweed polysaccharide is 18:1-3.
3. The microbial agent of claim 2, wherein: the seaweed polysaccharide has a water content of 10-20wt%; in the algal polysaccharide, the content of L-fucose is 20-30 wt%, the content of D-xylose is 15-25 wt%, the content of D-hexenuronic acid is 10-20 wt%, the content of sulfate is 8-15 wt% and the content of protein is 7-12% based on dry matter.
4. The microbial agent of claim 2, wherein: the content of the streptomyces grignathi in the microbial inoculum is 1 multiplied by 10 6 -3×10 8 cfu/g。
5. A microbial agent according to any one of claims 1 to 4, wherein: the plant is fructus Piperis, fructus Anisi Stellati, tea tree, fructus Tsaoko.
6. A microbial agent according to any one of claims 1 to 4, wherein: the crop diseases are pepper blast caused by phytophthora capsici, star aniseed root rot caused by fusarium oxysporum, tea tree southern blight caused by sclerotium rolfsii and tsaoko root rot caused by sclerotium rolfsii.
7. A method for promoting crop growth and/or controlling crop soil-borne diseases, characterized in that: the method comprising applying the microbial inoculum of any one of claims 1-4 to soil with a total amount of 1X 10 Streptomyces griseus 9 -5×10 12 cfu/strain/time, or the amount of the microbial inoculum applied to the soil is as follows: 300-500 g/strain/time.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117821341A (en) * | 2024-02-29 | 2024-04-05 | 云南省农业科学院农业环境资源研究所 | Streptomyces albus microbial inoculum and application thereof |
| CN117821341B (en) * | 2024-02-29 | 2024-05-28 | 云南省农业科学院农业环境资源研究所 | Streptomyces albus microbial inoculum and application thereof |
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