CN111040996B - 一种制备卵巢癌类器官的方法 - Google Patents
一种制备卵巢癌类器官的方法 Download PDFInfo
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- CN111040996B CN111040996B CN201911243577.4A CN201911243577A CN111040996B CN 111040996 B CN111040996 B CN 111040996B CN 201911243577 A CN201911243577 A CN 201911243577A CN 111040996 B CN111040996 B CN 111040996B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases (EC 2.)
- C12N2501/727—Kinases (EC 2.7.)
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- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/24—Genital tract cells, non-germinal cells from gonads
- C12N2502/243—Cells of the female genital tract, non-germinal ovarian cells
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- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/30—Coculture with; Conditioned medium produced by tumour cells
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- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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CN111607568A (zh) * | 2020-05-06 | 2020-09-01 | 苏州济研生物医药科技有限公司 | 一种原代卵巢癌细胞的培养方法及其在药物筛选中的应用 |
CN111849904B (zh) * | 2020-07-31 | 2021-05-04 | 浙江科途医学科技有限公司 | 神经母细胞瘤类器官的培养基、培养方法及移植体 |
CN115305240B (zh) * | 2022-09-30 | 2023-10-17 | 北京科途医学科技有限公司 | 一种培养类器官的方法和类器官的培养装置 |
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CN103497931B (zh) * | 2013-09-18 | 2015-11-18 | 广西医科大学 | 基于胶原水凝胶构建体内肿瘤工程化人卵巢癌肿瘤模型 |
CN105219730A (zh) * | 2015-11-10 | 2016-01-06 | 昭衍(苏州)新药研究中心有限公司 | 卵巢癌癌组织3d培养方法及其在药效评价中的应用 |
EP3530732A1 (en) * | 2018-02-23 | 2019-08-28 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Ovarian cancer organoid culture |
CN109392843A (zh) * | 2018-11-23 | 2019-03-01 | 上海美峰生物技术有限公司 | 基于类器官方法构建卵巢癌移植瘤模型及应用 |
CN109652376B (zh) * | 2019-01-08 | 2021-10-15 | 创芯国际生物科技(广州)有限公司 | 一种用于卵巢癌组织3d培养的培养基 |
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