CN111012702A

CN111012702A - Cosmetic composition containing herba Artemisiae Scopariae extract obtained by Heat-tin extraction method and low temperature aging as effective component

Info

Publication number: CN111012702A
Application number: CN201910960388.2A
Authority: CN
Inventors: 李在成; 赵奉相; 朴俊性; 任志玹
Original assignee: Best Solution Of Co Ltd; Dry Cloth Cosmetics Co Ltd; Leo Chemical Co Ltd Korea
Current assignee: Best Solution Of Co Ltd; Dry Cloth Cosmetics Co Ltd; Leo Chemical Co Ltd Korea
Priority date: 2018-10-10
Filing date: 2019-10-10
Publication date: 2020-04-17
Also published as: KR102011837B1

Abstract

The present invention relates to a cosmetic composition comprising as an active ingredient an extract of oriental wormwood prepared by Heat-tic extraction and low-temperature ripening, which has excellent effects of improving skin color, reducing the number of pores, anti-inflammatory and anti-oxidative, and is harmless to the human body without skin irritation. Furthermore, the Heat-tic extraction method overcomes the disadvantages of the conventional organic solvent extraction and hot water extraction, and thus can obtain effective components without removing the organic solvent, and can contribute to productivity and economy by reducing the preparation cost, and can maintain the storability and the inherent flavor of oriental wormwood by low-temperature ripening.

Description

Cosmetic composition containing herba Artemisiae Scopariae extract obtained by Heat-tin extraction method and low temperature aging as effective component

Technical Field

The present invention relates to a cosmetic composition comprising an extract of oriental wormwood as an active ingredient, and more particularly, to a non-irritating cosmetic composition comprising an extract of oriental wormwood prepared by a Heat-tin extraction method and low-temperature aging as an active ingredient and having excellent effects of improving skin color, reducing the number of pores, and anti-inflammatory and antioxidant effects.

Background

Artemisia capillaris (Artemisia capillaris) is a plant which is native in Korea, is a perennial herb belonging to Compositae (compositae), and is also called Artemisia capillaris and Artemisia tarragon. The herba Artemisiae Scopariae mainly uses tender bud, and has antiinflammatory, antibacterial, acne resisting, antioxidant, diuretic, and jaundice treating effects. It mainly contains chlorogenic acid (chlorogenic acid), caffeic acid (caffeic acid) as one of polyphenols, and oleic acid (oleic acid), linoleic acid (linoleic acid) as essential oil components.

On the other hand, the Heat-tic extraction method is an extraction method in which cold-maceration extraction and hot-water extraction are performed simultaneously and then mixed. Conventional extracts prepared using ethanol, ethyl acetate, or the like have excellent efficacy, but require a step of removing an organic solvent in order to be used as a cosmetic composition, and thus have a disadvantage of increasing preparation costs. Further, although the extract prepared by hot water extraction can be directly used as a cosmetic composition, there is a disadvantage in that the efficacy is reduced as compared with the extract extracted with an organic solvent. In order to solve the problem, a Heat-tic extraction method is newly researched and developed. The cold-soaking extraction of Heat-tic not only can obtain components having excellent effects like extraction using ethanol, ethyl acetate, etc., but also does not require a step of removing an organic solvent, and thus, this method is an extraction method that can make up for the disadvantages of organic solvent extraction and hot water extraction.

On the other hand, low-temperature ripening is a method for facilitating long-term storage without decomposing or destroying the precursor of the active ingredient obtained by extraction, and is a method often used in the food industry such as soy sauce, soybean paste, meat ripening, and the like. The cosmetic composition has the advantages that when the cosmetic composition is aged at a low temperature, the cosmetic composition can be stored for a long time without decomposing or destroying the active ingredients of the extract, and the inherent flavor of the extract can be maintained.

Documents of the prior art

Patent document

(patent document 1) Korean patent application No. 10-0855456 (granted on 26/08/2008)

Disclosure of Invention

Technical problem

The present invention aims to provide a cosmetic composition containing a Heat-tic extraction method and a low-temperature aged oriental wormwood extract as an active ingredient.

Another object of the present invention is to provide a method for preparing the above extract.

Means for solving the problems

In order to achieve the above object, the present invention provides a cosmetic composition comprising an extract of oriental wormwood as an active ingredient, which is prepared by a Heat-tic extraction method and low-temperature aging.

Also, the present invention provides a method for preparing an artemisia capillaris extract, comprising: the first step, finely cutting the oriental wormwood; a second step of adding an organic solvent to the finely cut artemisia capillaris and stirring at a temperature of 4 to 10 ℃ for 3 to 7 days to prepare a cold-dipped extract; a third step of adding water to the finely cut artemisia capillaris and stirring at a temperature of 45 to 100 ℃ for 3 to 7 days to prepare a hot water extract; a fourth step of preparing a mixed extract by mixing the cold-dipped extract of the second step and the hot-water extract of the third step; and a fifth step of subjecting the mixed extract to low-temperature ripening at a temperature of 4 ℃ to 15 ℃ for 2 days to 10 days.

ADVANTAGEOUS EFFECTS OF INVENTION

According to the present invention, the artemisia capillaris extract prepared by the Heat-tin extraction method and low-temperature aging has advantages of improving skin color, reducing the number of pores, having excellent anti-inflammatory and antioxidant effects, and being harmless to the human body without skin irritation, and thus can be effectively used as a cosmetic composition. Furthermore, the Heat-tic extraction method overcomes the disadvantages of the conventional organic solvent extraction and hot water extraction, and thus can obtain effective components without removing the organic solvent, and can contribute to productivity and economy by reducing the preparation cost, and can maintain the storability and the inherent flavor of oriental wormwood by low-temperature ripening.

Drawings

Fig. 1 is a graph for confirming cytotoxicity of artemisia capillaris thunb extract based on extraction solvent and mixing ratio by cell survival rate.

FIG. 2 is a graph showing the anti-inflammatory effects (NO, PGE2, IL-6) of Artemisia capillaris Thunb extract based on the extraction solvent and the mixing ratio, which were confirmed by enzyme-linked immunosorbent assay (ELISA).

FIG. 3 is a graph showing the anti-inflammatory effects (TNF- α, COX-2, IL-l β, iNOS) of Artemisia capillaris extract in real-time polymerase chain reaction (real-time PCR) based on the extraction solvent and the mixing ratio.

Fig. 4 is a graph for confirming the antioxidant effect of artemisia capillaris thunb extract based on the extraction solvent and the mixing ratio by the radical scavenging ability.

Fig. 5 is a graph showing confirmation of total polyphenol content of artemisia capillaris thunb extract based on extraction solvent and mixing ratio by DPPH analysis.

Fig. 6A to 6B are graphs for confirming the change in skin color caused by the artemisia capillaris extract, fig. 6A shows the degree of improvement in skin color, and fig. 6B shows the rate of improvement in skin color.

Fig. 7A to 7B are graphs confirming the change of the redness of the face caused by the artemisia capillaris extract, fig. 7A shows the degree of improvement of the redness of the face, and fig. 7B shows the improvement rate of the redness of the face.

Fig. 8A to 8B are graphs showing the change in the number of pores due to the artemisia capillaris extract, fig. 8A shows the degree of reduction in the number of pores that can be observed with the naked eye, and fig. 8B shows the reduction rate of the pore rate that can be observed with the naked eye.

Fig. 9 is a three-dimensional image showing the change in the number of pores caused by the artemisia capillaris extract.

Detailed Description

The present inventors have confirmed the advantages of artemisia capillaris thunb prepared separately as a cold-dipped extract or a hot-water extract, mixed and prepared an artemisia capillaris thunb extract by a Heat-tic extraction method, which has excellent effects of improving skin color, reducing the number of pores, anti-inflammatory and antioxidant effects, is harmless to the human body and has no skin irritation, and thus can be effectively utilized as a cosmetic composition, and have completed the present invention.

In view of the above, the present invention provides a cosmetic composition comprising an extract of oriental wormwood as an active ingredient, which is prepared by a Heat-tic extraction method and low-temperature aging.

The herba Artemisiae Scopariae extract is obtained by using one or more parts selected from leaf, flower, stem, and root which are collected in spring and dried to have the most effective medicinal effect. In this case, the extract can be obtained by pulverizing or finely cutting Artemisia capillaris into a size of 0.01mm to 2 cm. The "size" refers to a maximum length and a maximum width.

The extract of oriental wormwood comprises a solvent extract obtained by extracting oriental wormwood with an extraction solvent, a diluted solution or a concentrated solution of the solvent extract, a dried product obtained by drying the solvent extract, or a crude purified product or a purified product thereof, and the like, and may be a product fractionated by a solvent fractionation method, and may comprise a polar solvent fraction and a non-polar solvent fraction.

The composition can produce improved skin tone, improved facial redness, reduced pore count, anti-acne, anti-inflammatory and antioxidant effects without side effects and skin irritation.

The Heat-tic extraction method is a mixed cold-soaking extract and hot-water extract extraction method, and the ratio of 1: 0.2-1: the cold-dipped extract and the hot-water extract were mixed at a weight ratio of 0.8, but not limited thereto.

The cold-dipped extract may be extracted using a solvent selected from the group consisting of butylene glycol, propylene glycol and dipropylene glycol, but is not limited thereto.

The low-temperature ripening is performed for 2 to 10 days under a temperature condition of 4 to 15 ℃, but is not limited thereto.

The artemisia capillaris extract may be included by 80 parts by weight to 100 parts by weight with respect to 100 parts by weight of the total composition, but is not limited thereto.

In the case where the composition of the present invention is a cosmetic composition, the cosmetic composition may contain conventional adjuvants such as a stabilizer, a dissolving agent, vitamins, a pigment and a perfume, and a carrier, in addition to the effective ingredient. Also, the cosmetic composition may further comprise a skin absorption enhancer for enhancing the effect thereof.

As the formulation of the cosmetic composition, any formulation that is generally prepared in the art may be prepared, and for example, the cosmetic composition may be formulated into lotions, skin lotions, essence, sunscreen cream, foundation cream, pack, mask, gel, shampoo, hair rinse, spray, makeup remover, and cleansing agent, but is not limited thereto.

When the formulation is a solder paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide, etc. can be used as a carrier component.

In the case of a foundation or spray, as a carrier ingredient, lactose, talc, silicon dioxide, aluminum hydroxide, calcium silicate or polyamide powder can be used, and particularly in the case of a spray, a propellant such as chlorofluorocarbon, propane/butane or dimethyl ether can be further contained.

In the case where the formulation is a solution or emulsion, a solvent, a dissolving agent or an opacifying agent, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, 3-butylene glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan, may be used as a carrier component.

In the case where the dosage form is a suspension, as a carrier ingredient, a liquid phase diluent such as water, ethanol or propylene glycol; suspending agents such as ethoxylated isostearyl alcohols, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters; microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, tragacanth, or the like.

The organic solvent of the second step may be selected from the group consisting of butylene glycol, propylene glycol, and dipropylene glycol, but is not limited thereto.

In the fourth step, the ratio of 1: 0.2-1: the cold-dipped extract and the hot-water extract were mixed at a weight ratio of 0.8, but not limited thereto.

When the extraction temperature and the extraction time are applied, the extraction effect is excellent, the modification of the effective components is prevented, and the inherent flavor of the oriental wormwood can be maintained.

After the artemisia capillaris extract is ripened at a low temperature, it is extracted by one or more extraction methods selected from the group consisting of stirring extraction, reflux cooling extraction, ultrasonic extraction and supercritical extraction, and then filtered to prepare a filtrate.

And the step of obtaining the artemisia capillaris extract further comprises: a step of preparing a concentrate by concentration after aging at a low temperature; and a step of fractionating the concentrate with a fractionating solvent to prepare a fractionated extract.

The fractionated extract can be prepared by putting the concentrate into a funnel and putting a separating solvent, and the fractionating solvent may be one or more selected from the group consisting of water, acetone, ethyl acetate, butyl acetate, diethyl ether, benzene, chloroform, nucleic acid, and alcohol having 1 to 10 carbon atoms.

The step of preparing the fractionated extract comprises: a step of fractionating the concentrate with a first fractionating solvent to prepare a first fractionated extract; a step of fractionating said first fractionated extract with a second fractionated solvent to produce a second fractionated extract. When the fractionated extract is prepared by using the conditions, an artemisia capillaris extract having high contents of polyphenol, flavonoid compound and polysaccharide can be obtained.

The first and second fractionation solvents may be one or more selected from the group consisting of water, acetone, ethyl acetate, butyl acetate, diethyl ether, benzene, chloroform, nucleic acid, and alcohol having 1 to 10 carbon atoms. Preferably, the first fractionating solvent may include water, and the second fractionating solvent may include an alcohol having a carbon number of 1 to 10. The alcohol having 1 to 10 carbon atoms may include butanol (n-BuOH).

The present invention will be described in more detail below with reference to examples. These examples are only for illustrating the present invention more specifically, and it is obvious to those skilled in the art that the scope of the present invention is not limited to these examples according to the gist of the present invention.

Example 1: preparing herba Artemisiae Scopariae extract

Washing 300 parts by weight of whole plant of Artemisia capillaris (Korean medicinal herb) with purified water, drying, and finely cutting into 0.1-1 cm size with a blender.

The first extraction method is a cold soaking method, in which a solvent comprising 700 parts by weight of butylene glycol or propylene glycol and 500 parts by weight of water is added to the whole plant of finely cut oriental wormwood, the mixture is stirred at 5 ℃ for 5 days and extracted, and then filtered through whatman No.2 filter paper to obtain a filtrate, and the extraction and filtration are repeated 3 times in the same manner as described above.

The second extraction is a hot water extraction method, in which 500 parts by weight of water is added to the whole plant of finely cut oriental wormwood, the mixture is stirred at 70 ℃ for 3 days and extracted, and then filtered through whatman No.2 filter paper to obtain a filtrate, and the extraction and filtration are repeated 3 times in the same manner as described above.

Then, the ratio of 1: 0.4 or 1: 0.8 weight ratio of the extract extracted by the cold soaking extraction method to the extract extracted by the hot water extraction method and preparing the artemisia capillaris extract by the Heat-tic extraction method. Then, the extract of oriental wormwood was subjected to low temperature ripening at a temperature of 5 ℃ for 5 days to prepare a final extract of oriental wormwood.

TABLE 1

Example 2: skin irritation test Using Artemisia capillaris extract

Among persons who agreed to participate in the human body suitability test, 32 test subjects that met the selected criteria and did not belong to the exclusion criteria were subjected to the skin patch test using IQ ultimate. 25. mu.l of each application type (liquid phase, cream, etc.) test substance was dropped on UltimateTM, and the attachment type (mask, patch, etc.) test substance was cut into 1cm in width by 1cm in vertical direction to attach to the skin. The patch was attached for 24 hours, and after 1 hour of patch removal and 24 hours of patch removal, the degree of skin irritation was observed by 2 experts at a skin irritation index according to the criteria of the International Contact Dermatitis Research Group (ICDRG) (FIG. 2).

TABLE 2

Index of skin irritation	Distinguishing
		0.00-0.25	Non-irritating
0.26-1.00	Weak irritation
		1.01-2.50	Moderate irritation
2.51-4.00	Strong irritation

As a result, as shown in the following table 3, no skin irritation was observed after 1 hour of patch removal and 24 hours of patch removal, and the results of the primary irritation test on skin patches, which was conducted in the human application test, showed a skin irritation index of 0.00, and thus it was judged as a non-irritating product according to the judgment standards.

TABLE 3

Example 3: anti-inflammatory and antioxidant effects of Artemisia capillaris Thunb extract

3-1. cytotoxicity assay

RAW264.7 macrophages (ATCC) were counted using a hemocytometer (hemacytometer) at 1 × 10 in 96-well plates⁴The concentration of cells/well was seeded. After 24 hours of culture, macrophages were supplemented with the test substance or the comparative substance of example 1 at a total concentration of 5% per well using 100. mu.l of DMEM medium containing 10% fetal bovine serum and 5% penicillin/streptomycin at 37 DEG C5% of CO₂The culture was carried out in an incubator for 24 hours. After that, the supernatant was removed and added with 9: 1 weight ratio of DMEM medium to WST solution (EZ-cytox), 5% CO at 37 deg.C₂The reaction was carried out in an incubator for 2 hours, and the absorbance was measured at 450 nm. The degree of cytotoxicity (cell survival rate) was expressed in percent (%) based on the absorption intensity of a control group using solvents such as the test substance and the comparative substance.

Calculation formula 1: cell survival rate (%) (absorbance of sample substance added group/absorbance of control group X100)

As a result, it was confirmed with reference to fig. 1 that cytotoxicity did not occur in both the test substance and the comparative substance.

3-2 analysis of anti-inflammatory Effect (NO, PGE2, IL-6)

After counting RAW264.7 macrophages using a hemocytometer, the cells were plated at 2X 10 in 24-well plates⁵The concentration of cells/well was seeded. After 24 hours of culture, the test substance or comparative substance of example 1 was added to macrophages at a total concentration of 5% per well, using 1ml of DMEM medium containing 10% fetal bovine serum and 5% penicillin/streptomycin, and 5% CO at 37 deg.C₂The culture was carried out in an incubator for 24 hours. Using L-NMMA (N)^GMethyl-L-arginme, acetatesal) as a control group. After recovering the supernatant, nitric Oxide (NO, Intron 21021), prostaglandin E2(prostaglandin E2; PGE2, R were determined according to the protocol provided by the manufacturer&D systems KGE004B)、IL-6(R&D systems D6050)。

As a result, referring to fig. 2, it was confirmed that the test substance and the comparative substance both significantly reduced NO, PGE2, and IL-6, and among them, the test substance 1 exhibited the most excellent anti-inflammatory effect.

3-3 analysis of anti-inflammatory Effect (TNF- α, COX-2, IL-1 β, iNOS)

After counting RAW264.7 macrophages using a hemocytometer, the cells were plated at 2X 10 in 24-well plates⁵The concentration of cells/well was seeded. After 24 hours of culture, the test substance or comparative substance of example 1 was added to macrophages at a total concentration of 5% per well, using 1ml of a solution containing 10% fetal bovine serum and5% penicillin/streptomycin DMEM medium at 37 deg.C and 5% CO₂The culture was carried out in an incubator for 24 hours. Using L-NMMA (N)^GAfter recovering cells and extracting RNA using Nucleospin RNA kit, cDNA was synthesized, cDNA and Syber green (Bio-rad) TNF- α, cyclooxygenase-2 (COX-2; IL-1 β), Inducible Nitric Oxide Synthase (iNOS) primers (forward and reverse) were added, respectively, and real-time polymerase chain reaction (real-time PCR, Bio-rad) was performed, and the results were analyzed using software specific for real-time PCR (Bio-rad).

As a result, referring to FIG. 3, it was confirmed that the test substance and the comparative substance both significantly reduced TNF- α, COX-2, IL-1 β and iNOS, and among them, the anti-inflammatory effect of test substance 1 was the most excellent.

3-4 analysis of antioxidant Effect

DPPH (2, 2-Diphenyl-1-piperidinylhydrazyl) reagent was used to analyze the radical (free radial) scavenging ability. Ascorbic acid (ascorbyl acid) was used as a control. After adding the test substance or the comparative substance and ethanol to 0.1mM DPPH and reacting them in a dark room for 30 minutes, the absorbance at 520nm was measured.

Calculation formula 2: radical scavenging activity (%) -100-absorbance of sample substance addition group/absorbance of control group. times.100

As a result, referring to fig. 4, it was confirmed that both the test substance and the comparative substance produced radical scavenging ability, and among them, the test substance 1 exhibited the most excellent antioxidant effect.

Then, to measure the total polyphenol content, 0.1mL of a test substance or a comparative substance sample was mixed with 2mL of 7% Na₂CO₃(Sodium carbonate, Sigma-Aldrich) solution, after 3 minutes, 0.1ml of 1N Folin-Ciocalteu's phenol reagent (Sigma-Aldrich) was added and the reaction was carried out at room temperature for 30 minutes, and then the absorbance was measured at 750 nm. Ascorbic acid was used as a standard substance.

As a result, as can be seen from fig. 5, the total polyphenol content increased in both the test substance and the comparative example, and the total polyphenol content was the highest in the test substance 1.

Example 4: herba Artemisiae Scopariae extract with effects of improving skin color, improving facial redness, and reducing pore number

The essence product was prepared with the test substance 1 having the best effect in the above-described tests, and 21 adults aged 9 to 59 were used as subjects to perform the effect tests of improving skin color, improving redness of the face, and reducing the number of pores.

The degree of effects of improving skin color, improving face redness and reducing the number of pores of a face is evaluated by using a spectrophotometer (Chromameter) with skin brightness (L-value) and face redness (a-value) values, and after an image of the skin surface is photographed by using a three-dimensional image photographing apparatus, a three-dimensional reconstructed image is obtained by using a program. Quantitative analysis is performed on the number (Count) occupied by pores above a certain size existing in the selected field of the three-dimensional reconstructed image.

Calculation formula 3: skin color improvement ratio (%) (measurement value after sample application-measurement value before sample application)/measurement value before sample application × 100

Calculation formula 4: improvement in facial redness (%) (measured after sample application-measured before sample application)/measured before sample application × 100

Calculation formula 5: number of holes reduction (%) (measured after sample application-measured before sample application)/measured before sample application × 100

As a result, referring to fig. 6A to 6B, the skin color measurement values increased by 1.01%, 2.43%, and 2.25% in the forehead, left cheek, and right cheek test sites, respectively, after 2 weeks of application, and increased by 2.56%, 3.40%, and 3.34% in the forehead, left cheek, and right cheek test sites, respectively, after 4 weeks of application, compared to before application of the artemisia capillaris extract, and it was confirmed that the skin color of the test sites was improved.

Referring to fig. 7A to 7B, the measured values of redness were reduced by 7.51%, 6.77%, and 6.30% at the forehead, left cheek, and right cheek test sites, respectively, after 2 weeks of application, and by 9.40%, 9.56%, and 9.38% at the forehead, left cheek, and right cheek test sites, respectively, after 4 weeks of application, as compared to before application of the artemisia capillaris extract, and it was confirmed that the redness at the test sites was improved.

Further, referring to fig. 8A to 8B and fig. 9, the number of pores visually observed was decreased by 4.07% and 3.65% in the left and right test sites, respectively, after 2 weeks of application, and by 9.15% and 7.75% in the left and right test sites, respectively, after 4 weeks of application, compared to the number of pores visually observed in the test site before application of the artemisia capillaris extract.

That is, it was confirmed that the artemisia capillaris extract had an effect of improving skin color, facial redness, and the number of pores, that is, the skin lightness (L-value) increased at a statistically significant level (P <0.05), and the facial redness (a-value) and the number of pores (Pore count) decreased at a statistically significant level (P <0.05), from 2 weeks after the artemisia capillaris extract was applied to the test site.

Formulation example 1 formulation example of cosmetic composition

Formulation examples 1-1 preparation of skin lotion

Mixing and stirring 3.0 parts by weight of propylene glycol, 0.1 part by weight of carboxyl polymer, a trace amount of preservative and the balance of purified water, heating at a temperature of 80-85 ℃, putting into a preparation part, then starting an emulsifying machine, and heating and putting into the preparation part, 1.0 part by weight of polysorbate 60, 0.5 part by weight of sorbitan sesquioleate, 10.0 parts by weight of liquid paraffin, 1.0 part by weight of sorbitan stearate, 0.5 part by weight of lipophilic glyceryl monostearate, 1.5 parts by weight of stearic acid, 1.0 part by weight of glyceryl stearate/PEG-400 stearate, and 0.2 part by weight of triethanolamine at a temperature of 80-85 ℃ for emulsification. If emulsification is finished, stirring with a stirrer, heating and cooling to 50 deg.C, adding a small amount of perfume, cooling to 45 deg.C, adding a small amount of pigment, adding herba Artemisiae Scopariae extract at 35 deg.C, cooling to 25 deg.C, and aging.

Formulation examples 1-2 preparation of cream

0.3 part by weight of a carboxyl polymer, 5.0 parts by weight of butanediol, 3.0 parts by weight of glycerin and the balance of purified water were mixed and stirred, heated at a temperature of 80 to 85 ℃ and put into a preparation part, and then an emulsifying machine was started, and 2.0 parts by weight of stearic acid, 2.0 parts by weight of cetyl alcohol, 2.0 parts by weight of glyceryl monostearate, 0.5 parts by weight of polyoxyethylene sorbitan monostearate, 0.5 parts by weight of sorbitan sesquioleate, 1.0 parts by weight of glyceryl monostearate/glyceryl stearate/polyoxyethylene stearate, 1.0 part by weight of wax, 4.0 parts by weight of liquid paraffin, 4.0 parts by weight of squalane, and 4.0 parts by weight of caprylic/capric triglyceride were heated at a temperature of 80 to 85 ℃ and put into the mixture, and then 0.5 parts by weight of triethanolamine was put into the mixture and emulsified. If emulsification is finished, stirring with a stirrer, cooling to 35 deg.C, adding herba Artemisiae Scopariae extract, cooling to 25 deg.C, and aging.

While certain features of the invention have been described in detail above, it will be apparent to those skilled in the art that this detailed description is merely a preferred example and that the scope of the invention is not limited thereto. Accordingly, the substantial scope of the present invention is defined by the appended claims and equivalents thereof.

It should be construed that the scope of the present invention is indicated by the scope of the claims, and all modifications or variations derived from the meaning, scope and equivalent concept of the claims are included in the scope of the present invention.

Claims

1. A cosmetic composition comprises an extract of Artemisia capillaris Thunb prepared by Heat-tin extraction and low-temperature aging as an active ingredient.

2. The cosmetic composition of claim 1, wherein said composition has skin tone improving, facial redness improving, pore number reducing, anti-inflammatory and antioxidant effects.

3. The cosmetic composition according to claim 1, wherein in the Heat-tic extraction, a cold-dipped extract and a hot-water extract are mixed.

4. A cosmetic composition according to claim 3, characterized in that the ratio of 1: 0.2-1: 0.8 weight ratio of the cold-soaked extract and the hot-water extract.

5. The cosmetic composition according to claim 3, wherein the cold-dipped extract is extracted with a solvent selected from the group consisting of butylene glycol, propylene glycol and dipropylene glycol.

6. The cosmetic composition as claimed in claim 1, wherein the low temperature aging is performed for 2 to 10 days under a temperature condition of 4 to 15 ℃.

7. The cosmetic composition according to claim 1, wherein the artemisia capillaris extract comprises 80 to 100 parts by weight relative to 100 parts by weight of the composition.

8. A preparation method of a herba artemisiae scopariae extract is characterized by comprising the following steps:

the first step, finely cutting the oriental wormwood;

a second step of adding an organic solvent to the finely cut artemisia capillaris and stirring at a temperature of 4 to 10 ℃ for 3 to 7 days to prepare a cold-dipped extract;

a third step of adding water to the finely cut artemisia capillaris and stirring at a temperature of 45 to 100 ℃ for 3 to 7 days to prepare a hot water extract;

a fourth step of preparing a mixed extract by mixing the cold-dipped extract of the second step and the hot-water extract of the third step; and

a fifth step of subjecting the mixed extract to low-temperature ripening at a temperature of 4 to 15 ℃ for 2 to 10 days.

9. The method of preparing artemisia capillaris extract according to claim 8, wherein the organic solvent of the second step is selected from the group consisting of butylene glycol, propylene glycol and dipropylene glycol.

10. The method for preparing an artemisia capillaris extract according to claim 8, wherein the ratio of 1: 0.2-1: mixing the cold-dipped extract and the hot-water extract of the fourth step at a weight ratio of 0.8.