CN110988177A - 一种磷酰胆碱吗啉的高效液相色谱检测方法 - Google Patents

一种磷酰胆碱吗啉的高效液相色谱检测方法 Download PDF

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CN110988177A
CN110988177A CN201911299589.9A CN201911299589A CN110988177A CN 110988177 A CN110988177 A CN 110988177A CN 201911299589 A CN201911299589 A CN 201911299589A CN 110988177 A CN110988177 A CN 110988177A
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phosphorylcholine
morpholine
high performance
performance liquid
detection method
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嵇春波
宋敬宁
邱晓岩
付萌
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Pony Test Co
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    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
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Abstract

本发明属于分析化学技术领域，涉及一种磷酰胆碱吗啉的高效液相色谱检测方法。样液经十八烷基键合相的色谱柱分离，甲醇和水等度洗脱，蒸发光检测。本方法简单便捷，可快速测定磷酰胆碱吗啉含量，具有较高的准确性、稳定性和分离度。

Description

一种磷酰胆碱吗啉的高效液相色谱检测方法
技术领域
本发明属于分析化学技术领域，涉及一种磷酰胆碱吗啉的高效液相色谱检测方法，尤其是涉及一种十八烷基键合相的色谱柱分离、蒸发光检测的高准确性、稳定性和分离度的磷酰胆碱吗啉的高效液相色谱检测方法。
背景技术
胆碱类药物主要是针对胆碱受体、乙酰胆碱酯酶而开发的药品，分为拟胆碱药物、抗胆碱药物两大类。拟胆碱药物可用于青光眼、肠麻痹、血管痉挛性疾病等治疗；抗胆碱药物可用于抢救感染中毒性休克，解除有机磷农药中毒，阿斯综合症和内脏绞痛，也可用于麻醉前给药、散瞳或治疗角膜炎、虹膜炎等。
磷酰胆碱吗啉是合成胆碱类药物的中间体，不仅在生产前需要对其进行质量控制，而且在生产后也要监控其是否残留。然而目前并没有磷酰胆碱吗啉检测方法的相关研究。因此亟需开发磷酰胆碱吗啉的检测方法。
本方法简单便捷，可快速测定磷酰胆碱吗啉含量，具有较高的准确性、稳定性和分离度。
发明内容
本发明的目的是提供一种十八烷基键合相的色谱柱分离、蒸发光检测的高准确性、稳定性和分离度的磷酰胆碱吗啉的高效液相色谱检测方法。
为了实现本发明的目的，本发明的技术方案如下：试样经流动相溶解并定容，过膜后注入高效液相色谱仪，在设置仪器条件下采集色谱图，外标法定量；十八烷基键合相的色谱柱分离，柱温30～40℃，体积比为4/96～10/90的甲醇和水为流动相进行等度洗脱，柱流量0.7～1.0mL/min，进样体积10～20μL，蒸发光检测器检测。
有益效果：本发明采用十八烷基键合相的色谱柱分离，蒸发光检测，可快速测定磷酰胆碱吗啉含量，具有较高的准确性、稳定性和分离度，且方法简单便捷。
附图说明
图1样品溶液的高效液相色谱图
具体实施方式
结合实施例对本发明做进一步说明，但不以任何方式对本发明加以限制，基于本发明教导所做出的任何变更或改进，均属于本发明的保护范围。
实施例一
采用的高效液相色谱仪，配蒸发光检测器。
精密称取磷酰胆碱吗啉待测样品0.1g(精确0.001g)，用流动相溶解并定容至50mL。采用的色谱条件为：ODS-3色谱柱；40℃柱温；甲醇和水为流动相等度洗脱且体积比为4/96：1.0mL/min流速：10μL进样体积：蒸发光检测器检测。将样品溶液过膜后注入高效液相色谱仪，记录色谱图。4.19min为磷酰胆碱吗啉谱峰，保留时间适宜且分离度高，峰形对称性良好。

Claims (1)

1.一种磷酰胆碱吗啉的高效液相色谱检测方法，其特征在于，试样经流动相溶解并定容，过膜后注入高效液相色谱仪，在设置仪器条件下采集色谱图，外标法定量；十八烷基键合相的色谱柱分离，柱温30～40℃，体积比为4/96～10/90的甲醇和水为流动相进行等度洗脱，柱流量0.7～1.0mL/min，进样体积10～20μL，蒸发光检测器检测。
CN201911299589.9A 2019-12-12 2019-12-12 一种磷酰胆碱吗啉的高效液相色谱检测方法 Pending CN110988177A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112213437A (zh) * 2020-09-28 2021-01-12 诺安实力可商品检验（青岛）有限公司 一种宠物食品中橙色b的hplc和lc-ms/ms的检测方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4129660A (en) * 1975-07-16 1978-12-12 Agence Nationale De Valorisation De La Recherche (Anvar) Derivatives of cis-epoxy-1,2-propyl-phosphonic acid and drugs containing in particular as active ingredients derivatives of cis-epoxy-1,2-propylphosphonic acid in dextrorotatory form
WO2009085096A2 (en) * 2007-12-05 2009-07-09 Semprus Biosciences Corporation Non-leaching, non-fouling antimicrobial coatings
CN106565771A (zh) * 2016-11-10 2017-04-19 山东师范大学 一种含有双氨基的磷酰胆碱化合物Lys‑PC及其制备方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4129660A (en) * 1975-07-16 1978-12-12 Agence Nationale De Valorisation De La Recherche (Anvar) Derivatives of cis-epoxy-1,2-propyl-phosphonic acid and drugs containing in particular as active ingredients derivatives of cis-epoxy-1,2-propylphosphonic acid in dextrorotatory form
WO2009085096A2 (en) * 2007-12-05 2009-07-09 Semprus Biosciences Corporation Non-leaching, non-fouling antimicrobial coatings
CN106565771A (zh) * 2016-11-10 2017-04-19 山东师范大学 一种含有双氨基的磷酰胆碱化合物Lys‑PC及其制备方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112213437A (zh) * 2020-09-28 2021-01-12 诺安实力可商品检验（青岛）有限公司 一种宠物食品中橙色b的hplc和lc-ms/ms的检测方法
CN112213437B (zh) * 2020-09-28 2021-04-23 诺安实力可商品检验（青岛）有限公司 一种宠物食品中橙色b的hplc和lc-ms/ms的检测方法

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