CN110974773A

CN110974773A - Skin care product for anti-allergic repair and preparation method thereof

Info

Publication number: CN110974773A
Application number: CN201911376724.5A
Authority: CN
Inventors: 谭啸; 谭新民
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-12-27
Filing date: 2019-12-27
Publication date: 2020-04-10

Abstract

The invention discloses a skin care product for anti-allergic repair and a preparation method thereof, belonging to the technical field of cosmetics. A skin care product for anti-allergy repair comprising the following components: the composition comprises lyophilized powder of stem cell exocrine factor, lyophilized powder of stem cell inclusion lysis factor, hyaluronic acid, butanediol, olive leaf extract, purslane extract, evening primrose extract, chamomile hydrosol and purified water. The stem cell secretion factors and stem cell inclusion lysis factors contained in the skin care product are prepared by freeze-drying cell culture solution and cell suspension at low temperature, and various stem cell secretion factors contained in the skin care product can promote the repair and regeneration of cells; the olive leaf extract, the purslane extract, the evening primrose extract and the chamomile hydrosol can relieve the symptoms of sensitive skin and improve the skin resistance. The skin care product can quickly and effectively relieve anaphylactic reaction, improve the body resistance and play a role in repairing.

Description

Skin care product for anti-allergic repair and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a skin care product for anti-allergic repair and a preparation method thereof.

Background

With the development of society and the continuous improvement of living standard, the requirements of people on living quality are continuously improved, and more consumers use cosmetics for daily skin care. Not only women have the demand, but also skin care products gradually become life necessities of men, even the old and children. Among the wide applicable population, there are a few special populations sensitive to cosmetics, and after the ordinary cosmetics are used, the skin care product is easy to have stabbing pain, red swelling, itching and fever, and severe symptoms such as dry scraps, rash, dermatitis, blisters, ulcers and the like can be caused.

Facial skin allergy is a very common form of allergy, with skin allergy occurring in about 20% of people worldwide. In many daily cosmetics, various types of thickeners, chemical perfumes, artificial colors, preservatives, and the like are contained to a greater or lesser extent, and these substances are easily a source of skin allergy.

The natural anti-allergy and anti-irritation additive is extracted from natural plants, and is far better than a chemical synthetic material in the aspect of safety. Under the condition of huge consumption of the existing skin care cleaning products, the development and application of natural anti-allergic components are particularly important. Products with antiallergic effect are increasingly favored, and substances with antiallergic activity, in particular additives from pure plant sources, are sought after by the market and by the users.

Disclosure of Invention

The invention provides a skin care product for anti-allergy repair and a preparation method thereof, aiming at the problems that the existing skin care product is single in effective component and contains a sensitization source in raw materials. The skin care product of the invention adopts the natural anti-allergy and anti-stimulation additive to act synergistically with the stem cell exocrine factor and the stem cell inclusion lysis factor, so that the skin care product can fully exert the anti-allergy and repairing skin care effects.

In order to achieve the purpose, the invention adopts the following technical scheme:

a skin care product for anti-allergic repair comprises the following raw material components in percentage by mass, wherein the sum of the mass percentages is 100 percent: 0.1-0.5% of lyophilized powder of stem cell exocrine factor, 0.1-0.5% of lyophilized powder of stem cell inclusion lysis factor, 5-15% of hyaluronic acid, 5-15% of butanediol, 0.5-1% of olive leaf extract, 0.5-2% of purslane extract, 0.2-2% of evening primrose extract, 10-20% of pure dew and 65.5-57.7% of purified water.

The sodium hyaluronate is formed by mixing high-molecular sodium hyaluronate and low-molecular sodium hyaluronate in equal mass; the high molecular hyaluronic acid is hyaluronic acid with the molecular weight range of 1800000-2200000; the low molecular weight hyaluronic acid is hyaluronic acid with the molecular weight range of 400000-1000000.

The pure dew is flos Matricariae Chamomillae pure dew or flos Rosae Rugosae pure dew.

A preparation method of a skin care product for anti-allergy repair comprises the following steps:

(1) subculturing the human umbilical cord mesenchymal stem cells to P3 generation, and collecting the cells and cell culture solution when the coverage rate of the cells of P3 generation is more than or equal to 90% in a culture bottle;

(2) freeze-drying the cell culture solution collected in the step (1) to obtain stem cell secretion factor freeze-dried powder;

(3) resuspending, crushing and freeze-drying the cells collected in the step (1) to obtain dry cell inclusion lysis factor freeze-dried powder;

(4) mixing hyaluronic acid, butanediol, folium Canarii albi extract, herba Portulacae extract, radix Oenotherae Erythrosepalae extract, flos Matricariae Chamomillae hydrolat and purified water according to raw material component ratio, and preparing into dry cell factor solvent;

(5) adding the stem cell secretion factor freeze-dried powder and the stem cell inclusion lysis factor freeze-dried powder into the stem cell factor solvent obtained in the step (4) according to the raw material component proportion to prepare the anti-allergic repair skin care product.

The culture medium of the human umbilical cord mesenchymal stem cells in the step (1) is a Lonza serum-free culture medium, and the culture conditions are that the temperature is 37 ℃, the concentration of carbon dioxide is 5% and the humidity is 99%.

Compared with the prior art, the invention has the beneficial effects that:

the stem cell secretion factor contained in the skin care product is prepared by freeze-drying a cell culture solution at a low temperature, and the stem cell content lysis factor is obtained by freeze-drying at a low temperature after being crushed by ultrasonic waves, so that the activity of various stem cell secretion factors contained in the skin care product is fully ensured. Mainly comprises the following factors:

① Epidermal Growth Factor (EGF) content is 60-90pg/ml, and can promote growth, division and metabolism of epidermal cells, epithelial cells and fibroblasts in vivo, promote growth of microvasculature, and improve microenvironment for cell growth.

② fibroblast (FGF) content of 40-60pg/ml, it can promote the migration of endothelial cells and the proliferation of smooth muscle cells, and can also promote the formation of cardiovascular system and repair damaged cells, so it has good repairing effect on red blood streak, roughness, desquamation and pruritus after skin damage.

③ Transforming Growth Factor (TGF) content is 10-20pg/ml, it is involved in many cellular processes in mature organisms and developing embryos.

④ Keratinocyte Growth Factor (KGF) content is 20-40pg/ml, it can promote formation of intercellular substance, synthesis and secretion of collagen, increase intercellular matrix, make skin tender, body-building, plump and elastic, reduce wrinkle, promote proliferation and differentiation of epithelial cells, protect radiation, maintain cytoskeleton stability, and is a good sunscreen component.

⑤ Vascular Endothelial Growth Factor (VEGF) content is 60-90pg/ml, the epidermal tissue of skin has no vascular structure, the supply of nutrients and metabolite excretion mainly pass through dermal microvasculature, and VEGF can effectively increase the permeability of local blood vessel, so that abundant blood circulation provides sufficient nutrients and other growth factors for fibroblast proliferation and collagen synthesis, further promotes cell division and proliferation, improves skin microcirculation, and makes yellow, lusterless and unhealthy skin become ruddy and glossy.

Butanediol, olive leaf extract, purslane extract, evening primrose extract, hyaluronic acid, hydrolat and the like are used as solvents, so that the activity of stem cell secretory factors can be kept for a long time, solutes and various components in the solvents do not react with each other, the skin care effect of the stem cell secretory factors is fully exerted, the allergic reaction can be quickly and effectively relieved, the body resistance is improved, and the repairing effect can be achieved.

Detailed Description

In order to make the present invention more comprehensible, the technical solutions of the present invention are further described below with reference to specific embodiments, but the following examples are only examples of the present invention and do not represent the scope of the present invention defined by the claims.

Example 1:

a skin care product for anti-allergic repair comprises the following raw material components in percentage by mass, wherein the sum of the mass percentages is 100 percent: 0.1% of lyophilized powder of stem cell exocrine factor, 0.5% of lyophilized powder of stem cell inclusion lysis factor, 10% of hyaluronic acid, 10% of butanediol, 1% of olive leaf extract, 0.5% of purslane extract, 0.2% of evening primrose extract, 20% of chamomile hydrolat and 57.7% of purified water.

A method for preparing skin care product for anti-allergy repair comprises the following steps;

(1) subculturing human umbilical cord mesenchymal stem cells to P3 generation, culturing the cells of P3 generation in a culture bottle, and collecting cell culture solution when the coverage rate reaches 90%; the culture medium of the human umbilical cord mesenchymal stem cells is a Lonza serum-free culture medium, and the culture conditions of the human umbilical cord mesenchymal stem cells are that the temperature is 37 ℃, the concentration of carbon dioxide is 5 percent, and the humidity is 99 percent;

(2) freeze-drying the cell culture solution collected in the step (1) to obtain stem cell secretory factor powder;

(4) mixing hyaluronic acid, hydrolat, butanediol, folium Canarii albi extract, herba Portulacae extract, and radix Oenotherae Erythrosepalae extract with purified water according to raw material component ratio, and preparing into stem cell factor solvent; the hyaluronic acid is formed by mixing high molecular hyaluronic acid, low molecular hyaluronic acid and the like; the polymer hyaluronic acid is hyaluronic acid with the molecular weight range of 1800000-2200000; the low molecular weight hyaluronic acid is hyaluronic acid with the molecular weight range of 400000-1000000. The hydrolat is chamomile hydrolat.

(5) And (4) adding the stem cell factor powder into the stem cell factor solvent obtained in the step (4) according to the proportion of the raw material components to prepare the anti-allergic repair skin care product containing the stem cell exocrine and inclusion lysis factor.

Example 2:

a skin care product for anti-allergic repair comprises the following raw material components in percentage by mass, wherein the sum of the mass percentages is 100 percent: 0.5% of lyophilized powder of stem cell exocrine factor, 0.5% of lyophilized powder of stem cell inclusion lysis factor, 15% of hyaluronic acid, 5% of butanediol, 1% of olive leaf extract, 2% of purslane extract, 0.5% of evening primrose extract, 10% of chamomile hydrolat and 65.5% of purified water.

(5) And (4) adding stem cell factor powder into the stem cell factor solvent obtained in the step (4) according to the raw material component ratio to prepare the anti-allergic repair stem cell exocrine and inclusion lysis factor skin care product.

Example 3:

a skin care product for anti-allergic repair comprises the following raw material components in percentage by mass, wherein the sum of the mass percentages is 100 percent: 0.5% of lyophilized powder of stem cell exocrine factor, 0.1% of lyophilized powder of stem cell inclusion lysis factor, 5% of hyaluronic acid, 15% of butanediol, 0.5% of olive leaf extract, 1% of purslane extract, 2% of evening primrose extract, 15% of chamomile hydrolat and 60.9% of purified water.

(4) uniformly mixing 5% of hyaluronic acid, 15% of hydrolat, 15% of butanediol, 0.5% of olive leaf extract, 1% of purslane extract and 2% of evening primrose extract with purified water according to the proportion of the raw material components to prepare a dry cell factor solvent; the hyaluronic acid is formed by mixing high molecular hyaluronic acid, low molecular hyaluronic acid and the like; the polymer hyaluronic acid is hyaluronic acid with the molecular weight range of 1800000-2200000; the low molecular weight hyaluronic acid is hyaluronic acid with the molecular weight range of 400000-1000000. The hydrolat is chamomile hydrolat.

(5) And (3) adding 0.5% of dry cell factor powder into the dry cell factor solvent obtained in the step (4) according to the proportion of the raw material components to prepare the anti-allergic repair skin care product containing the stem cell exocrine and inclusion lysis factor.

Investigation of use effect:

the using method comprises the following steps: after cleaning and wiping the face every morning and evening, respectively taking 2mL of the skin care product in the examples 1-3, uniformly coating the skin care product on the face, slightly beating the face for 1min to completely absorb the skin care product, observing the face after 1 month, counting, taking 100 examples, and simultaneously setting 3 groups of control examples, namely a control example 1, a control example 2 and a control example 3.

Table 1 investigation of the use of the skin care product of the present invention

Note: the control examples 1-3 did not contain lyophilized powder of stem cell exocrine factor and lyophilized powder of stem cell inclusion lysis factor, and the other conditions were the same as those in examples 1-3.

As can be seen from Table 1, in each of 100 trial cases, at least 87 of the facial allergy phenomena in examples 1-3 were alleviated; at least 92 cases of the skin redness were significantly reduced; at least 35 cases of skin become obviously fine; at least 78 cases 8 had marked fading of the acne marks and the mottle; at least 49 pore reductions; at least 95 cases of facial skin are obviously fine and glossy. Compared with the control example, the cases of obviously slowing down the phenomena of facial discomfort and allergy, obviously relieving the phenomenon of skin redness, refining the skin, gradually fading acne marks and color spots, shrinking pores and obviously refining and glossing the facial skin are obviously higher than the control example. After the skin care product for anti-allergic repair by using the stem cell exocrine and inclusion lysis factor is used for one month, the facial skin allergy can be obviously improved and repaired.

The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.

Claims

1. The skin care product for anti-allergy repair is characterized by comprising the following raw material components in percentage by mass, wherein the sum of the percentage by mass is 100 percent: 0.1-0.5% of lyophilized powder of stem cell exocrine factor, 0.1-0.5% of lyophilized powder of stem cell inclusion lysis factor, 5-15% of hyaluronic acid, 5-15% of butanediol, 0.5-1% of olive leaf extract, 0.5-2% of purslane extract, 0.2-2% of evening primrose extract, 10-20% of pure dew and 65.5-57.7% of purified water.

2. The skin care product for antiallergic restoration according to claim 1, characterized in that: the sodium hyaluronate is formed by mixing high molecular sodium hyaluronate and low molecular sodium hyaluronate in equal mass; the molecular weight range of the high molecular sodium hyaluronate is 1800000-2200000; the molecular weight range of the low-molecular sodium hyaluronate is 400000-1000000 hyaluronic acid.

3. The skin care product for antiallergic restoration according to claim 1, characterized in that: the pure dew is flos Matricariae Chamomillae pure dew or flos Rosae Rugosae pure dew.

4. The method for preparing a skin care product for antiallergic restoration according to claim 1, characterized by comprising the steps of;

(1) subculturing the human umbilical cord mesenchymal stem cells to P3 generation, and collecting the cells and cell culture solution when the coverage rate of the P3 generation cells is more than or equal to 90% in a culture bottle;

(5) adding the stem cell secretion factor freeze-dried powder and the stem cell inclusion lysis freeze-dried powder into the stem cell factor solvent obtained in the step (4) according to the raw material component proportion to prepare the anti-allergic repair stem cell exocrine and inclusion lysis factor skin care product.

5. The method for preparing a skin care product for antiallergic restoration according to claim 4, characterized in that: the culture medium of the human umbilical cord mesenchymal stem cells in the step (1) is a Lonza serum-free culture medium, and the culture conditions are that the temperature is 37 ℃, the concentration of carbon dioxide is 5 percent, and the humidity is 99 percent.