CN110897033A - Compound microbial feed additive and preparation method thereof - Google Patents
Compound microbial feed additive and preparation method thereof Download PDFInfo
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Abstract
The embodiment of the invention discloses a compound microbial feed additive and a preparation method thereof, and particularly relates to the technical field of feed additives, wherein the used raw materials comprise, by weight, 8-10 parts of sweet potatoes, 8-10 parts of pumpkins, 10-14 parts of rosa roxburghii tratt, 15-20 parts of collard, 15-20 parts of common yam rhizome, 15-20 parts of gentian, 8-12 parts of garden burnet, 20-30 parts of agaric, 0.1-0.3 part of lactic acid bacteria, 0.1-0.4 part of bifidobacteria, 0.2-0.4 part of bacillus subtilis, 0.1-0.5 part of enterococcus faecalis and 0.2-0.5 part of lactobacillus helveticus. According to the invention, the sweet potatoes, pumpkins, rosa roxburghii tratt, kale, common yam rhizome, bacillus subtilis, enterococcus faecalis and lactobacillus helveticus are added to effectively improve the immunity of animals, so that the growth and development of the animals are facilitated, and the balance of normal bacterial flora in intestinal tracts can be maintained, the growth of pathogenic bacteria is inhibited, constipation is prevented and diarrhea is treated by adding the roots of gentian, garden burnet, agaric, lactobacillus and bifidobacterium.
Description
Technical Field
The embodiment of the invention relates to the technical field of feed additives, in particular to a compound microorganism feed additive and a preparation method thereof.
Background
The feed is a general term of food of all animals raised by people, and in a narrower sense, the general feed mainly refers to food of animals raised in agriculture or animal husbandry, and the feed comprises more than ten kinds of feed raw materials such as soybean, soybean meal, corn, fish meal, amino acid, miscellaneous meal, whey powder, grease, meat and bone meal, grains, feed additives and the like. Generally, only plant feeds are referred to as feeds, and these feeds include grasses, various grains, tubers, roots, and the like. These feeds can be broadly divided into: feed containing a large amount of starch: these feeds are mainly composed of grains, seeds and roots or tubers containing a large amount of starch, and these feeds are mainly supplied with energy by polysaccharides and contain little protein. Oil-containing feed: these feeds consist of oil-containing seeds and the energy source for these feeds is mainly from lipids and therefore their energy density is higher than that of starch-containing feeds. Protein-containing feed: the feed is mainly plant protein feed mainly containing protein mulberry, and is the best raw material for replacing imported plant protein. Green feed: these feeds, in which the whole plant is fed, contain a large amount of carbohydrates and are very nutritious.
The feed additive refers to a small amount or trace substances added in the processes of processing, preparing and using the feed, and comprises nutritional feed additives and general feed additives. The feed additive is a raw material inevitably used in modern feed industry, and has obvious effects on strengthening the nutritive value of basic feed, improving the production performance of animals, ensuring the health of the animals, saving the feed cost, improving the quality of animal products and the like. With the development of multiple disciplines such as animal nutrition, physiology, feeding science, biochemistry, bioengineering, pharmacology, microbiology and the like, the current feed additive integrates multiple disciplines and multiple new technologies. Therefore, the development and production of the feed additive will present the development direction of science and technology and specialization at present and in some future.
The prior art has the following defects: in the early growth stage of animals, the immunity of the animals is low, and the animals are easy to be infected with diseases and die, so that the breeding cost is easily increased, and the problem that how to enhance the autoimmunity of the animals in practical feed is a problem to be solved urgently is needed, so that the invention of the compound microorganism feed additive and the preparation method thereof is necessary.
Disclosure of Invention
Therefore, the embodiment of the invention provides a compound microorganism feed additive and a preparation method thereof, the added sweet potatoes are rich in vitamin C, the added pumpkin polysaccharide rich in pumpkin is a nonspecific immunity reinforcing agent, the immunity function of the organism can be improved, the generation of cell factors can be promoted, meanwhile, the rich carotenoid in pumpkin can be converted into vitamin A with important physiological functions in the organism, so that the compound microorganism feed additive has important physiological functions on the growth and differentiation of epithelial tissues, the maintenance of normal vision and the promotion of the development of bones, the added roxburgh rose contains rich vitamin C, the added collard contains a large amount of vitamin A, C, B2 and various mineral substances, the added common yam rhizome contains a large amount of vitamin C, inorganic salt, trace elements and other nutritional ingredients, thereby effectively helping the animals supplement the vitamin C and promoting the immunity of the animals, the added bacillus subtilis can improve the levels of immune globulin and antibody and improve the immunity of the colony, the added enterococcus faecalis can generate natural antibiotic, is beneficial to the health of an organism, can also generate antibacterial substances such as bacteriocin and the like and inhibit the growth of pathogenic bacteria, and the added lactobacillus helveticus has the functions of promoting immunity, resisting bacteria and resisting viruses, so the immunity of animals is further improved by inhibiting the pathogenic bacteria in the animals, and the growth and development of the animals are facilitated.
In order to achieve the above object, the embodiments of the present invention provide the following technical solutions: a composite microbial feed additive comprises, by weight, 8-10 parts of sweet potatoes, 8-10 parts of pumpkins, 10-14 parts of rosa roxburghii tratt, 15-20 parts of collard, 15-20 parts of common yam rhizome, 15-20 parts of gentian, 8-12 parts of garden burnet, 20-30 parts of agaric, 0.1-0.3 part of lactic acid bacteria, 0.1-0.4 part of bifidobacteria, 0.2-0.4 part of bacillus subtilis, 0.1-0.5 part of enterococcus faecalis and 0.2-0.5 part of lactobacillus helveticus.
Further, the used raw materials (by weight portion) comprise 8 portions of sweet potatoes, 8 portions of pumpkins, 10 portions of rosa roxburghii tratt, 15 portions of kale, 15 portions of common yam rhizome, 15 portions of gentian, 8 portions of garden burnet, 20 portions of agaric vegetable, 0.1 portion of lactic acid bacteria, 0.1 portion of bifidobacteria, 0.2 portion of bacillus subtilis, 0.1 portion of enterococcus faecalis and 0.2 portion of lactobacillus helveticus.
Further, the used raw materials (by weight portion) comprise 9 portions of sweet potatoes, 9 portions of pumpkins, 12 portions of rosa roxburghii tratt, 17.5 portions of kale, 17.5 portions of common yam rhizome, 17.5 portions of gentian, 10 portions of garden burnet, 25 portions of agaric, 0.2 portion of lactic acid bacteria, 0.25 portion of bifidobacterium, 0.3 portion of bacillus subtilis, 0.3 portion of enterococcus faecalis and 0.35 portion of lactobacillus helveticus.
Further, the used raw materials (by weight portion) comprise 10 portions of sweet potatoes, 10 portions of pumpkins, 14 portions of rosa roxburghii tratt, 20 portions of kale, 20 portions of common yam rhizome, 20 portions of gentian, 12 portions of garden burnet, 30 portions of agaric, 0.3 portion of lactic acid bacteria, 0.4 portion of bifidobacteria, 0.4 portion of bacillus subtilis, 0.5 portion of enterococcus faecalis and 0.5 portion of lactobacillus helveticus.
The invention also comprises a preparation method of the compound microorganism feed additive, which comprises the following steps:
s1, processing raw materials, namely cleaning and chopping sweet potatoes, pumpkins, roxburgh roses, kale, common yam rhizome, gentian and agaric vegetables, cutting roots of garden burnet roots, cleaning and chopping;
s2, airing: drying the cleaned raw materials in the sun, and simultaneously drying the raw materials by using a fan;
s3, crushing and sterilizing: putting the dried raw materials into a grinder for grinding, sieving by a sieve, and then sterilizing at low temperature;
s4, mixing: mixing lactobacillus, bifidobacterium, bacillus subtilis, enterococcus faecalis and lactobacillus helveticus, then uniformly mixing with the sterilized raw materials, then adding water with the total mass percentage of 30-60%, and stirring to form a mixture;
s5, fermentation: placing the mixture in a closed fermentation tank for natural fermentation;
s6, drying and crushing: taking out the materials after fermentation, drying the materials by using a dryer, and then crushing by using a crusher to obtain the composite microbial feed additive.
Further, the airing time is 1 to 3 hours in step S2, and sterilization is performed using a low-temperature sterilization chamber in step S3.
Further, in step S5, the fermentation temperature is 30-40 ℃, and the fermentation time is 48-72 h.
Further, the moisture content of the material dried in the step S6 is 10-12%.
The embodiment of the invention has the following advantages:
1. the added sweet potatoes are rich in vitamin C, the added pumpkin polysaccharide is a non-specific immunopotentiator, the immunologic function of an organism can be improved, the generation of cell factors is promoted, meanwhile, the rich carotenoid in the pumpkin can be converted into vitamin A with an important physiological function in the organism, so that the vitamin A has important physiological functions on the growth and differentiation of epithelial tissues, the maintenance of normal vision and the development of bones, the added roxburgh rose called 'the king of vitamin C' contains rich vitamin C, the added collard is rich in nutrition and contains a large amount of vitamin A, C, B2 and various mineral substances, the added common yam rhizome contains a large amount of vitamin C, inorganic salt, trace elements and other nutritional ingredients, so that the vitamin C is effectively supplemented to the animal, the immunity of the animal is improved, and simultaneously the added bacillus subtilis can improve the immune globulin and antibody levels, the immunity of the colony is improved, the added enterococcus faecalis can generate natural antibiotics, is beneficial to the health of an organism, can generate antibacterial substances such as bacteriocin and the like at the same time, and can inhibit the growth of pathogenic bacteria, and the added lactobacillus helveticus has the functions of promoting immunity, resisting bacteria and resisting viruses, so that the immunity of the animal is further improved by inhibiting the pathogenic bacteria in the animal body, and the growth and development of the animal are facilitated;
2. according to the invention, the added gentian has the functions of clearing heat and drying dampness, the added sanguisorba root can astringe and stop diarrhea and inhibit various pathogenic microorganisms, the added agaric vegetable has the functions of lubricating intestines, dissipating heat and facilitating urination and defecation, the added lactobacillus has the function of promoting gastrointestinal peristalsis, and the added bifidobacterium can maintain normal bacterial flora balance of intestinal tracts, inhibit the growth of pathogenic bacteria, prevent constipation, treat diarrhea, effectively improve intestines and stomach of animals, and inhibit constipation and diarrhea of animals.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the invention provides a compound microbial feed additive, which comprises the following raw materials (by weight portion) 8-10 parts of sweet potato, 8-10 parts of pumpkin, 10-14 parts of roxburgh rose, 15-20 parts of collard, 15-20 parts of Chinese yam, 15-20 parts of gentian, 8-12 parts of garden burnet, 20-30 parts of edible tree fungus, 0.1-0.3 part of lactic acid bacteria, 0.1-0.4 part of bifidobacterium, 0.2-0.4 part of bacillus subtilis, 0.1-0.5 part of enterococcus faecalis and 0.2-0.5 part of lactobacillus helveticus.
And specifically in this embodiment: the raw materials comprise, by weight, 8 parts of sweet potatoes, 8 parts of pumpkins, 10 parts of roxburgh roses, 15 parts of collards, 15 parts of common yam rhizomes, 15 parts of felwort, 8 parts of sanguisorba officinalis, 20 parts of agaric vegetables, 0.1 part of lactic acid bacteria, 0.1 part of bifidobacteria, 0.2 part of bacillus subtilis, 0.1 part of enterococcus faecalis and 0.2 part of lactobacillus helveticus.
The preparation method of the compound microorganism feed additive comprises the following specific steps:
s1, processing raw materials, namely cleaning and chopping sweet potatoes, pumpkins, roxburgh roses, kale, common yam rhizome, gentian and agaric vegetables, cutting roots of garden burnet roots, cleaning and chopping;
s2, airing: drying the cleaned raw materials in the sun, and simultaneously drying the raw materials by using a fan for 1-3 h;
s3, crushing and sterilizing: putting the dried raw materials into a grinder for grinding, sieving by a sieve, and then sterilizing at low temperature by using a low-temperature sterilization box;
s4, mixing: mixing lactobacillus, bifidobacterium, bacillus subtilis, enterococcus faecalis and lactobacillus helveticus, then uniformly mixing with the sterilized raw materials, then adding water with the total mass percentage of 60%, and stirring to form a mixture;
s5, fermentation: placing the mixture in a closed fermentation tank for natural fermentation at 40 deg.C for 72 h;
s6, drying and crushing: and taking out the material after fermentation, drying the material by using a dryer to ensure that the water content of the material is 12%, and crushing by using a crusher to obtain the composite microbial feed additive.
Example 2:
the invention provides a compound microbial feed additive, which comprises the following raw materials (by weight portion) 8-10 parts of sweet potato, 8-10 parts of pumpkin, 10-14 parts of roxburgh rose, 15-20 parts of collard, 15-20 parts of Chinese yam, 15-20 parts of gentian, 8-12 parts of garden burnet, 20-30 parts of edible tree fungus, 0.1-0.3 part of lactic acid bacteria, 0.1-0.4 part of bifidobacterium, 0.2-0.4 part of bacillus subtilis, 0.1-0.5 part of enterococcus faecalis and 0.2-0.5 part of lactobacillus helveticus.
And specifically in this embodiment: the raw materials (by weight portion) include 9 portions of sweet potato, 9 portions of pumpkin, 12 portions of roxburgh rose, 17.5 portions of kale, 17.5 portions of common yam rhizome, 17.5 portions of Chinese gentian, 10 portions of garden burnet root, 25 portions of edible fungus, 0.2 portion of lactic acid bacteria, 0.25 portion of bifidobacterium, 0.3 portion of bacillus subtilis, 0.3 portion of enterococcus faecalis and 0.35 portion of lactobacillus helveticus.
The preparation method of the compound microorganism feed additive comprises the following specific steps:
s1, processing raw materials, namely cleaning and chopping sweet potatoes, pumpkins, roxburgh roses, kale, common yam rhizome, gentian and agaric vegetables, cutting roots of garden burnet roots, cleaning and chopping;
s2, airing: drying the cleaned raw materials in the sun, and simultaneously drying the raw materials by using a fan for 1-3 h;
s3, crushing and sterilizing: putting the dried raw materials into a grinder for grinding, sieving by a sieve, and then sterilizing at low temperature by using a low-temperature sterilization box;
s4, mixing: mixing lactobacillus, bifidobacterium, bacillus subtilis, enterococcus faecalis and lactobacillus helveticus, then uniformly mixing with the sterilized raw materials, then adding water with the total mass percent of 45%, and stirring to form a mixture;
s5, fermentation: placing the mixture in a closed fermentation tank for natural fermentation at 35 deg.C for 60 hr;
s6, drying and crushing: and taking out the material after fermentation, drying the material by using a dryer to ensure that the water content of the material is 11%, and crushing by using a crusher to obtain the composite microbial feed additive.
Example 3:
the invention provides a compound microbial feed additive, which comprises the following raw materials (by weight portion) 8-10 parts of sweet potato, 8-10 parts of pumpkin, 10-14 parts of roxburgh rose, 15-20 parts of collard, 15-20 parts of Chinese yam, 15-20 parts of gentian, 8-12 parts of garden burnet, 20-30 parts of edible tree fungus, 0.1-0.3 part of lactic acid bacteria, 0.1-0.4 part of bifidobacterium, 0.2-0.4 part of bacillus subtilis, 0.1-0.5 part of enterococcus faecalis and 0.2-0.5 part of lactobacillus helveticus.
And specifically in this embodiment: the raw materials comprise, by weight, 10 parts of sweet potatoes, 10 parts of pumpkins, 14 parts of roxburgh roses, 20 parts of collards, 20 parts of common yam rhizomes, 20 parts of felwort, 12 parts of sanguisorba officinalis, 30 parts of agaric vegetables, 0.3 part of lactic acid bacteria, 0.4 part of bifidobacteria, 0.4 part of bacillus subtilis, 0.5 part of enterococcus faecalis and 0.5 part of lactobacillus helveticus.
The preparation method of the compound microorganism feed additive comprises the following specific steps:
s1, processing raw materials, namely cleaning and chopping sweet potatoes, pumpkins, roxburgh roses, kale, common yam rhizome, gentian and agaric vegetables, cutting roots of garden burnet roots, cleaning and chopping;
s2, airing: drying the cleaned raw materials in the sun, and simultaneously drying the raw materials by using a fan for 1-3 h;
s3, crushing and sterilizing: putting the dried raw materials into a grinder for grinding, sieving by a sieve, and then sterilizing at low temperature by using a low-temperature sterilization box;
s4, mixing: mixing lactobacillus, bifidobacterium, bacillus subtilis, enterococcus faecalis and lactobacillus helveticus, then uniformly mixing with the sterilized raw materials, then adding water with the total mass percentage of 60%, and stirring to form a mixture;
s5, fermentation: placing the mixture in a closed fermentation tank for natural fermentation at 40 deg.C for 72 h;
s6, drying and crushing: and taking out the material after fermentation, drying the material by using a dryer to ensure that the water content of the material is 12%, and crushing by using a crusher to obtain the composite microbial feed additive.
Example 4:
the compound microorganism feed additives prepared in the above examples 1 to 3 were respectively used to prepare feeds, and 90 animals of the same species were bred, each 30 animals were used as one group, the feeds prepared by using the compound microorganism feed additives in the three examples were tried out in three groups, and after 3 months of breeding, the following data were obtained:
| number of animals raised | Average degree of reduction of animal disease probability | The average degree of reduction of the occurrence probability of constipation | Reduced average degree of diarrhea occurrence probability | |
| Example 1 | 30 | 27％ | 20％ | 31％ |
| Example 2 | 30 | 64％ | 54％ | 62％ |
| Example 3 | 30 | 38％ | 24％ | 34％ |
As can be seen from the above table, the feed prepared by using the composite microbial feed additive prepared in example 2 has a moderate mixing ratio, can effectively improve the immune function of an animal body, promote the generation of animal cell factors, and simultaneously can generate antibacterial substances such as bacteriocins and the like, and inhibit the growth of pathogenic bacteria in the animal body, so that the disease probability of the animal is reduced to the maximum extent, and simultaneously, the feed is beneficial to the growth and differentiation of animal epithelial tissues and the development of bones, and after the animal eats the feed prepared by using the composite microbial feed additive prepared in example 2, the probability of constipation and diarrhea is greatly improved, so that the intestinal environment is greatly improved, the normal intestinal flora balance is maintained, and the growth and development of the animal are facilitated.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (8)
1. A compound microorganism feed additive is characterized in that: the raw materials (by weight portion) include 8-10 portions of sweet potato, 8-10 portions of pumpkin, 10-14 portions of roxburgh rose, 15-20 portions of kale, 15-20 portions of common yam rhizome, 15-20 portions of gentian, 8-12 portions of garden burnet, 20-30 portions of agaric, 0.1-0.3 portion of lactic acid bacteria, 0.1-0.4 portion of bifidobacterium, 0.2-0.4 portion of bacillus subtilis, 0.1-0.5 portion of enterococcus faecalis and 0.2-0.5 portion of lactobacillus helveticus.
2. The compound microbial feed additive of claim 1, wherein: the raw materials comprise, by weight, 8 parts of sweet potatoes, 8 parts of pumpkins, 10 parts of roxburgh roses, 15 parts of collards, 15 parts of common yam rhizomes, 15 parts of felwort, 8 parts of sanguisorba officinalis, 20 parts of agaric vegetables, 0.1 part of lactic acid bacteria, 0.1 part of bifidobacteria, 0.2 part of bacillus subtilis, 0.1 part of enterococcus faecalis and 0.2 part of lactobacillus helveticus.
3. The compound microbial feed additive of claim 1, wherein: the raw materials (by weight portion) include 9 portions of sweet potato, 9 portions of pumpkin, 12 portions of roxburgh rose, 17.5 portions of kale, 17.5 portions of common yam rhizome, 17.5 portions of Chinese gentian, 10 portions of garden burnet root, 25 portions of edible fungus, 0.2 portion of lactic acid bacteria, 0.25 portion of bifidobacterium, 0.3 portion of bacillus subtilis, 0.3 portion of enterococcus faecalis and 0.35 portion of lactobacillus helveticus.
4. The compound microbial feed additive of claim 1, wherein: the raw materials comprise, by weight, 10 parts of sweet potatoes, 10 parts of pumpkins, 14 parts of roxburgh roses, 20 parts of collards, 20 parts of common yam rhizomes, 20 parts of felwort, 12 parts of sanguisorba officinalis, 30 parts of agaric vegetables, 0.3 part of lactic acid bacteria, 0.4 part of bifidobacteria, 0.4 part of bacillus subtilis, 0.5 part of enterococcus faecalis and 0.5 part of lactobacillus helveticus.
5. The compound microbial feed additive according to any one of claims 1 to 4, wherein: also comprises a preparation method of the compound microorganism feed additive, which comprises the following steps:
s1, processing raw materials, namely cleaning and chopping sweet potatoes, pumpkins, roxburgh roses, kale, common yam rhizome, gentian and agaric vegetables, cutting roots of garden burnet roots, cleaning and chopping;
s2, airing: drying the cleaned raw materials in the sun, and simultaneously drying the raw materials by using a fan;
s3, crushing and sterilizing: putting the dried raw materials into a grinder for grinding, sieving by a sieve, and then sterilizing at low temperature;
s4, mixing: mixing lactobacillus, bifidobacterium, bacillus subtilis, enterococcus faecalis and lactobacillus helveticus, then uniformly mixing with the sterilized raw materials, then adding water with the total mass percentage of 30-60%, and stirring to form a mixture;
s5, fermentation: placing the mixture in a closed fermentation tank for natural fermentation;
s6, drying and crushing: taking out the materials after fermentation, drying the materials by using a dryer, and then crushing by using a crusher to obtain the composite microbial feed additive.
6. The method for preparing the compound microorganism feed additive according to claim 5, wherein the method comprises the following steps: air-drying for 1-3h in step S2, and sterilizing with a low-temperature sterilizing chamber in step S3.
7. The method for preparing the compound microorganism feed additive according to claim 5, wherein the method comprises the following steps: in step S5, the fermentation temperature is 30-40 deg.C, and the fermentation time is 48-72 h.
8. The method for preparing the compound microorganism feed additive according to claim 5, wherein the method comprises the following steps: the water content of the dried material in the step S6 is 10-12%.
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