CN110881528A - Production technology of highland barley bean pollen rich in gamma-aminobutyric acid and ferulic acid - Google Patents

Production technology of highland barley bean pollen rich in gamma-aminobutyric acid and ferulic acid Download PDF

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Publication number
CN110881528A
CN110881528A CN201811078363.1A CN201811078363A CN110881528A CN 110881528 A CN110881528 A CN 110881528A CN 201811078363 A CN201811078363 A CN 201811078363A CN 110881528 A CN110881528 A CN 110881528A
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China
Prior art keywords
highland barley
soybean
gamma
ferulic acid
acid
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Pending
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CN201811078363.1A
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Chinese (zh)
Inventor
王沛
马燕
杨润强
顾振新
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Nanjing Agricultural University
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Nanjing Agricultural University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C11/00Milk substitutes, e.g. coffee whitener compositions
    • A23C11/02Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
    • A23C11/10Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins

Abstract

The invention relates to a production technology of highland barley soybean meal rich in gamma-aminobutyric acid and ferulic acid, which belongs to the technical field of food processing and is characterized in that highland barley and soybean seeds germinate to enrich gamma-aminobutyric acid and ferulic acid, the germinated highland barley and soybean seeds are taken as raw materials, and are subjected to root removal, cleaning, grinding, secondary homogenization, spray drying, compounding with maltodextrin, gluconolactone, sodium carboxymethylcellulose and carrageenan, sterilization and packaging to obtain the highland barley soybean meal. The highland barley and soybean pollen powder prepared by the invention is rich in nutrition and fine and smooth in taste, has the functions of treating insomnia, relieving anxiety, resisting oxidation, bacteria and viruses and the like, and is a health food with wide audience range and high nutritional value. The content of gamma-aminobutyric acid and ferulic acid in the product respectively reaches 300-500 mg/100g and 100-250 mg/100 g.

Description

Production technology of highland barley bean pollen rich in gamma-aminobutyric acid and ferulic acid
One, the technical field
The invention relates to a production technology of highland barley soybean meal rich in gamma-aminobutyric acid and ferulic acid, belonging to the technical field of food processing.
Second, background Art
Gamma-aminobutyric acid is a four-carbon non-protein amino acid, is an important nerve activity inhibitor in human bodies, has physiological effects of preventing arteriosclerosis, regulating arrhythmia, reducing blood fat and blood pressure, relieving anxiety, improving sleep and the like, and has certain effects on various mental diseases such as epilepsy, convulsion, Huntington's disease, Parkinson disease and the like.
Ferulic acid is a natural active substance widely existing in plants, and the application of ferulic acid is gradually expanded from the field of medicines to the fields of health care products, cosmetics and the like. Ferulic acid has various physiological effects, and can scavenge endogenous or exogenous active oxygen such as hydrogen peroxide, superoxide radical, hydroxyl radical, etc., and prevent atherosclerosis, cancer, etc. Ferulic acid has certain inhibitory effect on Escherichia coli, Bacillus subtilis, Staphylococcus aureus and yeast; ferulic acid can prevent platelet aggregation and thrombosis, and is beneficial to cardiovascular and cerebrovascular health. Ferulic acid also has effects of preventing bone loss, and improving climacteric syndrome. The grains and beans are daily main food, but the content of gamma-aminobutyric acid and ferulic acid in common grains is not high, the bioavailability is low, the body requirement is difficult to meet, after the grains and bean seeds germinate, endogenous enzyme is activated, functional components are obviously increased, the content of active components such as amino acid, soluble dietary fiber, vitamin C and antioxidant enzyme is obviously increased, and the nutritional value is improved.
At present, researches on tofu powder at home and abroad are mostly focused on two aspects: the first is to improve the process. The patent (publication No. CN 102948491A, published 2013, 03 and 06) discloses 'an instant and convenient tofu pudding powder and a preparation method thereof', which only adjusts the formula to facilitate brewing and does not relate to the aspect of nutrition enrichment; the second is a fine formula. The patent (publication No. CN 107518085A, published on 2017, 12 and 29) discloses a preparation method of instant chenopodium quinoa biochar bean milk uncongealed beancurd powder, and the patent (publication No. CN 107258916A, published on 2017, 10 and 20) discloses a preparation method of instant chenopodium quinoa tofu uncongealed beancurd powder, only the types and the compounding proportions of raw materials are adjusted, the current situation of the raw materials is maintained in functional components, and the preparation process of the raw materials is not adjusted and controlled, so that the functional components are enriched, and highland barley beancurd powder rich in gamma-aminobutyric acid and ferulic acid is not reported.
The invention adopts NaCl and CaCl2The germinated highland barley and soybean seeds are sprayed by the mixed aqueous solution, and the related synthetic pathways are activated, so that the gamma-aminobutyric acid and ferulic acid are enriched, and the health-care effect of the highland barley and soybean pollen is enhanced.
Third, the invention
Technical problem
The invention aims to provide a production technology of highland barley bean pollen rich in gamma-aminobutyric acid and ferulic acid, the product is rich in beneficial components such as gamma-aminobutyric acid, ferulic acid, dietary fiber, vitamins and the like, the functional characteristics of the bean pollen are enhanced, the formula is scientific, the process is advanced, and the industrial production is easy to realize.
Technical scheme
The technology of the invention is as follows: the semen Avenae Nudae and semen glycines grains are treated with NaCl and CaCl2Spraying the mixed aqueous solution for germination, and enriching gamma-aminobutyric acid and ferulic acid; the raw materials are pulped, homogenized and spray-dried, and then maltodextrin, gluconolactone, sodium carboxymethylcellulose and carrageenan are added, and the product is prepared by sterilization and packaging. The method specifically comprises the following steps:
(1) spray germination
A. Selecting raw materials: selecting highland barley and soybean seeds with normal color and complete particles;
B. disinfecting and soaking: respectively soaking and disinfecting the highland barley and the soybean seeds for 15min by using a 0.5% sodium hypochlorite aqueous solution, repeatedly washing the highland barley and the soybean seeds by using clear water until the pH value is neutral, and then soaking the highland barley and the soybean seeds in the clear water at the temperature of 20-30 ℃ for absorbing water for 6-10 h;
C. spraying and germinating: respectively placing the soaked highland barley and soybean seeds in a germination machine, and adding 50-100 mM NaCl and 1-6 mM CaCl at 15-35 deg.C2Spraying the mixed aqueous solution for sprouting for 20-48 h, wherein the bud length of the highland barley and the soybean reaches 1-3 mm;
(2) preparing fresh pulp: cleaning germinated highland barley and soybeans with purified water respectively, draining, mixing according to a ratio of 1: 5-1: 10(w/w), adding purified water into the mixture of the germinated highland barley and the germinated soybeans according to a material-water ratio (w/v) of 1: 6-1: 10, setting the rotation speed of a colloid mill to be 3000-5000 r/min, grinding for 8-15 min, sieving with a 100-150 mesh sieve, and filtering out residues to obtain highland barley soybean raw pulp;
(3) homogenizing and spray drying: carrying out primary homogenization on the highland barley soybean raw pulp under the pressure of 15-25 MPa, increasing the pressure to 30-40 MPa, and carrying out secondary homogenization at the temperature of 50-65 ℃; performing air-flow spray drying according to a known method, wherein the air inlet temperature is 150 ℃, the air blast amount is 80%, and the highland barley soybean raw powder with the moisture content of less than 3% is prepared;
(4) mixing and blending: adding 0.2-0.8% of maltodextrin, 0.3-0.6% of gluconolactone, 0.05-0.13% of sodium carboxymethylcellulose and 0.10-0.15% of carrageenan into the highland barley soybean raw powder according to the weight ratio (w/w), and uniformly mixing to obtain highland barley soybean powder;
(5) and (3) sterilization and packaging: the highland barley bean pollen is sterilized by microwave, the output power is 50-100 KW, the time is 5-10 min, then the highland barley bean pollen is packaged by a vacuum aluminum foil bag, and the contents of gamma-aminobutyric acid and ferulic acid in the product are 300-500 mg/100g and 100-250 mg/100g respectively.
Advantageous effects
Compared with the prior art, the invention has the following advantages:
(1) after the highland barley and soybean seeds are germinated by adopting a biotechnology, the beneficial components such as gamma-aminobutyric acid, ferulic acid, amino acid, vitamins and the like are obviously improved;
(2) the bean pollen is prepared from the highland barley and the soybean seeds, and the nutrition of the grains and the beans is complementary, so that the defect of single nutrition of the traditional bean pollen is overcome.
(3) The product prepared by the spray drying method has fine and smooth mouthfeel and is convenient to brew.
Fourth, detailed description of the invention
Example 1
Selecting highland barley and soybean seeds with normal color and complete particles, soaking the highland barley and soybean seeds in a 0.5% sodium hypochlorite solution for 15min for disinfection, cleaning the highland barley and soybean seeds, soaking the highland barley and soybean seeds in clear water at the temperature of 20-30 ℃ for 6-10 h, transferring the highland barley and soybean seeds into a germination machine, and soaking the highland barley and soybean seeds in 60mM NaCl and 2mM CaCl at the temperature of 25 ℃ for 6-10 h2Spraying the mixed aqueous solution for germination for 48 h; cleaning highland barley and soybean seeds with the germ length of 2.0-2.5 mm, draining, uniformly mixing according to a ratio of 1: 8(w/w), adding purified water into a mixture of germinated highland barley and germinated soybean according to a ratio of material to water (w/v) of 1: 7, setting the rotation speed of a colloid mill to 4000r/min, grinding for 10min, and filtering and removing residues by using 120-mesh filter cloth to obtain highland barley soybean raw stock; homogenizing the primary pulp at 20MPa, and performing secondary homogenization at 35MPa, wherein the homogenization temperature is 55 ℃; then spray drying is carried out, the air inlet temperature is 150 ℃, the air blast amount is 80%, and the highland barley and soybean raw powder is prepared; adding 0.6 percent of maltodextrin and glucose into the highland barley and soybean raw powder according to the weight ratio (w/w)Uniformly mixing 0.5% of acid lactone, 0.11% of sodium carboxymethylcellulose and 0.12% of carrageenan to obtain highland barley soybean meal; sterilizing at 50-100 KW for 5-10 min, and packaging with vacuum aluminum foil bags. The content of gamma-aminobutyric acid in the product reaches 450mg/100g, and the content of ferulic acid reaches 220mg/100 g.
Example 2
The method for selecting, disinfecting and soaking the highland barley seeds is the same as that of the embodiment 1, and then the highland barley seeds are transferred to a germination machine, and the highland barley seeds are treated by NaCl and CaCl at the temperature of 20 DEG C2Spraying the mixed aqueous solution for germination for 36h, and germinating the soybean seeds for 24h at 30 ℃ in the same culture solution; cleaning highland barley and soybean seeds with the germ length of 1.5-2.0 mm, draining, uniformly mixing according to a ratio of 1: 9(w/w), adding purified water into a mixture of germinated highland barley and germinated soybean according to a material-water ratio (w/v) of 1: 8, grinding into slurry, and filtering to obtain highland barley soybean raw slurry as in example 1; the homogenization and spray drying methods were the same as in example 1; adding 0.5% of maltodextrin, 0.4% of gluconolactone, 0.10% of sodium carboxymethylcellulose and 0.14% of carrageenan into the highland barley soybean raw powder according to the weight ratio (w/w), and uniformly mixing to obtain highland barley soybean powder; the sterilization and packaging methods were the same as in example 1. The content of gamma-aminobutyric acid in the product reaches 400mg/100g, and the content of ferulic acid reaches 200mg/100 g.
Example 3
The method for selecting, disinfecting and soaking the highland barley seeds is the same as that of the embodiment 1, and then the highland barley seeds are transferred to a germination machine, and the highland barley seeds are treated by NaCl and CaCl at the temperature of 15 DEG C2Spraying the mixed aqueous solution for germination for 36h, and germinating the soybean seeds for 24h at 25 ℃ in the same culture solution; cleaning highland barley and soybean seeds with the germ length of 1.5-2.5 mm, draining, uniformly mixing according to a ratio of 1: 10(w/w), adding purified water into a mixture of germinated highland barley and germinated soybean according to a material-water ratio (w/v) of 1: 9, grinding into slurry, and filtering to obtain highland barley soybean raw slurry as in example 1; homogenizing, spray drying, mixing, sterilizing, and packaging in the same manner as in example 1. The content of gamma-aminobutyric acid in the product reaches 320mg/100g, and the content of ferulic acid reaches 150mg/100 g.
The embodiments of the present invention have been described in detail above, but this is only an example for easy understanding and should not be construed as limiting the scope of the present invention. Also, various equivalent changes or substitutions are possible for those skilled in the art according to the technical solution of the present invention and the description of the preferred embodiment thereof, but all such changes or substitutions shall fall within the protection scope of the claims of the present invention.

Claims (2)

1. A production technology of highland barley soybean pollen rich in gamma-aminobutyric acid and ferulic acid is characterized in that highland barley and soybean seeds are sprayed and germinated by culture solution to obtain germinated highland barley and soybean rich in gamma-aminobutyric acid and ferulic acid, after pulping, homogenizing and spray drying, highland barley soybean raw powder is compounded with maltodextrin, gluconolactone, sodium carboxymethylcellulose and carrageenan, and the highland barley soybean pollen is prepared by sterilizing and packaging, and specifically comprises the following steps:
(1) spray germination
A. Selecting raw materials: selecting highland barley and soybean seeds which are full in grains, free of diseases and insect pests and high in germination rate;
B. disinfecting and soaking: respectively soaking the highland barley and the soybean seeds in 0.5 percent sodium hypochlorite aqueous solution for 15min according to a known disinfection method, washing the highland barley and the soybean seeds to be neutral by clear water, and then soaking the highland barley and the soybean seeds in the clear water at the temperature of 20-30 ℃ for absorbing water for 6-10 h;
C. spraying and germinating: respectively placing the soaked highland barley and soybean seeds in a germination machine, and adding 50-100 mM NaCl and 1-6 mM CaCl at 15-35 deg.C2Spraying the mixed aqueous solution for sprouting for 20-48 h, wherein the bud length of the highland barley and the soybean reaches 1-3 mm;
(2) preparing fresh pulp: cleaning germinated highland barley and soybeans with purified water, mixing according to a ratio of 1: 5-1: 10(w/w), adding purified water into the mixture of the germinated highland barley and the germinated soybeans according to a material-water ratio (w/v) of 1: 6-1: 10, setting the rotation speed of a colloid mill to be 3000-5000 r/min, grinding for 8-15 min, then sieving with a 100-150 mesh sieve, and filtering out residues to obtain highland barley soybean raw pulp;
(3) homogenizing and spray drying: carrying out primary homogenization on the highland barley soybean raw pulp under the pressure of 15-25 MPa, increasing the pressure to 30-40 MPa, and carrying out secondary homogenization at the temperature of 50-65 ℃; performing air-flow spray drying according to a known method, wherein the air inlet temperature is 150 ℃, and the air blast amount is 80%, so as to obtain highland barley soybean raw powder;
(4) mixing and blending: adding 0.2-0.8% of maltodextrin, 0.3-0.6% of gluconolactone, 0.05-0.13% of sodium carboxymethylcellulose and 0.10-0.15% of carrageenan into the highland barley soybean raw powder according to the weight ratio (w/w) and uniformly mixing to obtain highland barley soybean powder;
(5) and (3) sterilization and packaging: and (3) carrying out microwave sterilization on the highland barley bean pollen for 5-10 min according to a known sterilization method, and then packaging the product by using a vacuum aluminum foil bag.
2. The production technology of highland barley pollen containing rich gamma-aminobutyric acid and ferulic acid as claimed in claim 1, wherein the raw material highland barley is common highland barley or colored highland barley, the raw material soybean is soybean with yellow skin, black skin and green peel, and the content of gamma-aminobutyric acid and ferulic acid in the product is 300-500 mg/100g and 100-250 mg/100g respectively.
CN201811078363.1A 2018-09-11 2018-09-11 Production technology of highland barley bean pollen rich in gamma-aminobutyric acid and ferulic acid Pending CN110881528A (en)

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CN112089013A (en) * 2020-03-31 2020-12-18 青海汉和生物科技股份有限公司 Production method of germinated black highland barley powder rich in gamma-aminobutyric acid
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CN115720990A (en) * 2022-12-10 2023-03-03 东北农业大学 Sugar-free high-nutrition instant quick-setting soybean pollen prepared from germinated soybeans

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CN112089013A (en) * 2020-03-31 2020-12-18 青海汉和生物科技股份有限公司 Production method of germinated black highland barley powder rich in gamma-aminobutyric acid
CN112088943A (en) * 2020-03-31 2020-12-18 青海汉和生物科技股份有限公司 Black highland barley milk tea rich in gamma-aminobutyric acid and preparation method thereof
CN111700201A (en) * 2020-06-18 2020-09-25 南京农业大学 Production process of highland barley solid beverage rich in flavone
CN114515253A (en) * 2022-01-23 2022-05-20 广州清淞泉生物科技有限公司 Stable anti-aging and whitening composition and preparation method and application thereof
CN114515253B (en) * 2022-01-23 2022-11-11 苏州日月年世生物科技有限公司 Stable anti-aging and whitening composition and preparation method and application thereof
CN115720990A (en) * 2022-12-10 2023-03-03 东北农业大学 Sugar-free high-nutrition instant quick-setting soybean pollen prepared from germinated soybeans

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