CN107410508A - A kind of production method of the fermented soybean milk rich in gamma aminobutyric acid - Google Patents
A kind of production method of the fermented soybean milk rich in gamma aminobutyric acid Download PDFInfo
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- CN107410508A CN107410508A CN201710312901.8A CN201710312901A CN107410508A CN 107410508 A CN107410508 A CN 107410508A CN 201710312901 A CN201710312901 A CN 201710312901A CN 107410508 A CN107410508 A CN 107410508A
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- Prior art keywords
- aminobutyric acid
- rich
- soybean milk
- fermented soybean
- fermented
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C11/00—Milk substitutes, e.g. coffee whitener compositions
- A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
- A23C11/10—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
- A23C11/103—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
- A23C11/106—Addition of, or treatment with, microorganisms
Abstract
The invention discloses a kind of production method of the fermented soybean milk rich in gamma aminobutyric acid, this method is rich in gamma aminobutyric acid sprouted broad bean as raw material using soybean kernel after hypoxemia soaks, again through what salt stress joint calcium chloride germinateed to obtain, the Lactobacillus plantarum of inoculation production gamma aminobutyric acid after defibrination compounding, again through lactic fermentation, allotment, homogeneous, sterilizing and filling, the fermented soybean milk rich in gamma aminobutyric acid is made, gamma aminobutyric acid content is up to 15~30 mg/100mL in product.Present invention process is simple, and industrialization degree is high, and products obtained therefrom mouthfeel is excellent, has the physiological function of gamma aminobutyric acid and fermented soybean milk concurrently, nutritive value is high, is a kind of preferable functional beverage.
Description
Technical field
The invention belongs to food processing technology field, and in particular to a kind of fermented soybean milk rich in γ-aminobutyric acid and its
Production technology.
Background technology
Soybean is global vegetable crop, is also one of generalized grain crop of China seven, and it has yield high and nutritious
The features such as, it is protein and origin of amino acid important in human diet.Protein content is up to 30~40% in soybean, its seed
After grain germination, endogenous enzyme system is activated, and can not only reduce the ANFs content such as phytic acid and tannin, and protein be hydrolyzed to it is tired
Product γ-aminobutyric acid（GABA）The nitrogen substance of abundance is provided, has been used for functional food of the exploitation rich in GABA.GABA is
A kind of nonprotein amino acid, is widely present in animal and plant body, is that mammal inhibiting nerve transmits material.GABA is in people
Played an important role in brain energy metabolism, have activation intracerebral glucose metabolism, promote acetylcholine synthesis, subtract blood ammonia, it is anticonvulsion,
Hypotensive, the different physiological roles such as brain function and accelerating alcohol metabolism are improved, are a kind of new resource foods.
Germinated soybean forms soy yogurt through lactobacillus-fermented, can significantly reduce the intrinsic beany flavor of soybean, improves mouthfeel
Flavor, while reduce and easily cause the oligosaccharide content of flatulence, and organic acid is produced to suppress the saprophytic bacteria growing of enteron aisle, having improves
Human body functions of intestines and stomach, improve the physiological functions such as immunity function.During soybean kernel germinates, especially coerced in hypoxemia, low temperature and salt
Compel to wait and germinateed under abiotic stress, its raising of GABA content number in terms of again.And it is raw material to utilize the sprouted broad bean rich in GABA, use
Energy high yield GABA Lactobacillus plantarum fermentation is remarkably improved GABA content in fermented soybean milk, so as to reach the work(of double health
Effect.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of production method of the fermented soybean milk rich in γ-aminobutyric acid,
Using hypoxemia saline sook and the obtained sprouted broad bean rich in γ-aminobutyric acid of stress germination as matrix, with reference to highly producing gamma-aminobutyric acid
Lactobacillus plantarum, fermented soybean milk of the production rich in γ-aminobutyric acid, collection soybean nutritional, lactic acid bacteria and its metabolin health care are made
With the physiological function with γ-aminobutyric acid in one, have and reduce blood pressure, adjust blood fat and improve the functions such as sleep.
In order to solve the above technical problems, the present invention adopts the following technical scheme that：A kind of fermentation beans rich in γ-aminobutyric acid
The production method of breast, soaked with soybean kernel through hypoxemia, be rich in gamma-amino fourth through what salt stress joint calcium chloride germinateed to obtain again
The sprouted broad bean of acid be raw material, the Lactobacillus plantarum of inoculation highly producing gamma-aminobutyric acid after defibrination compounding, again fermented, allotment, homogeneous,
Sterilizing and packaging, are made the fermented soybean milk rich in γ-aminobutyric acid.
Further, the production method of the fermented soybean milk rich in γ-aminobutyric acid specifically includes following steps：
（1）Sterilization immersion:Full grains are selected, the soybean kernel of no disease and pests harm, with sodium hypochlorite soaking disinfection, then uses and steams
Distilled water cleans up, and is subsequently placed in 5~10 times of volumes（V/W）20~40mmol/L saline solutions in, in 25~32 DEG C of conditions
3~8 h of lower immersion, are passed through air during immersion, throughput is 2.0~5.0L/min；
（2）Stress germination：Soybean kernel after immersion is germinateed 12~24h under the conditions of 25~33 DEG C, every 2h during germination
Spray mixed aqueous solution 1 time, 50~80mmol/L sodium chloride and 5~10mmol/L calcium chloride are contained in mixed aqueous solution, to bud
Long 1~10mm, is made the sprouted broad bean rich in γ-aminobutyric acid；
（3）Defibrination compounds：Obtained sprouted broad bean is removed slag obtained soya-bean milk by known method defibrination；Add 2~6%（W/V）Breast
Sugar, heated by known method and sterilize and adjust pH as 6.0~7.5；
（4）Lactic fermentation：The Lactobacillus plantarum of highly producing gamma-aminobutyric acid is pressed known actication of culture method and activated 2 times obtained two
Level seed liquor；By 3~6%（V/V）Secondary seed solution is inoculated with soya-bean milk, hair is made in the 4~12h that fermented under the conditions of 33~40 DEG C
Ferment soya-bean milk；
（5）Allocate homogeneous, sterilization filling：By fermented soybean milk by known allotment homogeneous and the processing of sterilization filling method, richness is made
Fermented soybean milk containing γ-aminobutyric acid.
Alpha-aminobutyric acid content is 15~30 mg/100mL in fermented soybean milk produced by the present invention.
Beneficial effects of the present invention：The production method of fermented soybean milk of the present invention rich in γ-aminobutyric acid has
Advantages below：
（1）Lactobacillus plantarum of the present invention using the sprouted broad bean rich in γ-aminobutyric acid as raw material combination highly producing gamma-aminobutyric acid produces
Fermented soybean milk, the nutritive value of product is lifted, have γ-aminobutyric acid and double health effect of soy yogurt concurrently；
（2）The present invention combines salt stress by hypoxemia during immersion and handled, and is combined during germination by salt stress at calcium chloride
Reason, realizes γ-aminobutyric acid efficiently concentrating in a length of 1~10mm of bud sprouted broad bean.
Embodiment
Technical scheme will be clearly and completely described by embodiment below.
Embodiment 1
Full grains are selected, the soybean kernel of no disease and pests harm, is placed in 1.5% liquor natrii hypochloritis and soaks 15 min, then with steaming
Distilled water is cleaned, until neutral pH；It is placed in 6 times of volumes（V/W）40 mmol/L saline solutions in, in 32 DEG C soak 3 h, immersion
Period is continually fed into air, throughput 5.0L/min；Then soybean is placed in 28 DEG C of 18 h of germination, sprayed during germination every 2h
Leaching contains 50mmol/L sodium chloride and 5mmol/L calcium chloride waters 1 time；Sprouted broad bean defibrination is removed slag obtained soya-bean milk, adds 5%（W/
V）Lactose, heating, which is sterilized, simultaneously adjusts pH as 6.0~7.5；By 6%（V/V）Be inoculated with activation Lactobacillus plantarum secondary seed solution in
In soya-bean milk, 40 DEG C of fermentation 4h are placed in, it is well mixed after 65 that emulsifying agent, stabilizer, essence and sucrose are added after 4 DEG C of curings overnight
DEG C, pressure 25Mpa homogeneous is twice, filling after 140 DEG C of ultra high temperature short time sterilization 5s coolings, obtained fermented soybean milk, in product
Alpha-aminobutyric acid content is 20 mg/100mL.
Embodiment 2
Full grains are selected, the soybean kernel of no disease and pests harm, is placed in 1.5% liquor natrii hypochloritis and soaks 15 min, then with steaming
Distilled water is cleaned, until neutral pH；It is placed in 5 times of volumes（V/W）20 mmol/L saline solutions in, in 25 DEG C soak 8 h, immersion
Period is continually fed into air, throughput 2.0L/min；Then soybean is placed in 26 DEG C of 24 h of germination, sprayed during germination every 2h
Leaching contains 80mmol/L sodium chloride and 10mmol/L calcium chloride waters 1 time；Sprouted broad bean defibrination is removed slag obtained soya-bean milk, adds 4%
（W/V）Lactose, heating, which is sterilized, simultaneously adjusts pH as 6.0~7.5；By 4%（V/V）It is inoculated with the Lactobacillus plantarum secondary seed of activation
Liquid is placed in 33 DEG C of fermentation 12h in soya-bean milk, and emulsifying agent, stabilizer, essence and sucrose are added after 4 DEG C of curings overnight and is well mixed
After 65 DEG C, pressure 25Mpa homogeneous is twice, filling after 140 DEG C of ultra high temperature short time sterilization 5s coolings, and fermented soybean milk is made,
Alpha-aminobutyric acid content is 25 mg/100mL in product.
Embodiment 3
Full grains are selected, the soybean kernel of no disease and pests harm, is placed in 1.5% liquor natrii hypochloritis and soaks 15 min, then with steaming
Distilled water is cleaned, until neutral pH；It is placed in 10 times of volumes（V/W）30 mmol/L saline solutions in, in 28 DEG C soak 6 h, immersion
Period is continually fed into air, throughput 4.0L/min；Then soybean is placed in 33 DEG C of 12 h of germination, sprayed during germination every 2h
Leaching contains 70mmol/L sodium chloride and 8mmol/L calcium chloride waters 1 time；Sprouted broad bean defibrination is removed slag obtained soya-bean milk, adds 3%（W/
V）Lactose, heating, which is sterilized, simultaneously adjusts pH as 6.0~7.5；By 4%（V/V）Be inoculated with activation Lactobacillus plantarum secondary seed solution in
In soya-bean milk, 36 DEG C of fermentation 7h are placed in, it is well mixed after 65 that emulsifying agent, stabilizer, essence and sucrose are added after 4 DEG C of curings overnight
DEG C, pressure 25Mpa homogeneous is twice, filling after 140 DEG C of ultra high temperature short time sterilization 5s coolings, obtained fermented soybean milk, in product
Alpha-aminobutyric acid content is 15 mg/100mL.
Have been described in detail above embodiments of the present invention, but this is only to facilitate the example for understanding and lifting, should not be by
It is considered as and limits the scope of the present invention.Equally, any person of ordinary skill in the field can the technique according to the invention
Scheme and its description of preferred embodiment, make various possible equivalent changes or replace, but all these changes or replacement are all
The scope of the claims of the present invention should be belonged to.
Claims (3)
- A kind of 1. production method of the fermented soybean milk rich in γ-aminobutyric acid, it is characterised in that：Soaked with soybean kernel through hypoxemia Bubble, the sprouted broad bean rich in γ-aminobutyric acid for germinateing to obtain through salt stress joint calcium chloride again are raw material, are inoculated with after defibrination compounding high The Lactobacillus plantarum of γ-aminobutyric acid, again fermented, allotment, homogeneous, sterilizing and packaging are produced, is made and is rich in γ-aminobutyric acid Fermented soybean milk.
- A kind of 2. production method of fermented soybean milk rich in γ-aminobutyric acid according to claim 1, it is characterised in that： Specifically include following steps：1）Sterilization immersion:Full grains are selected, the soybean kernel of no disease and pests harm, with sodium hypochlorite soaking disinfection, then use distillation Water cleans up, and is subsequently placed in 5~10 times of volumes（V/W）20~40mmol/L saline solutions in, under the conditions of 25~32 DEG C 3~8 h are soaked, air are passed through during immersion, throughput is 2.0~5.0L/min；2）Stress germination：Soybean kernel after immersion is germinateed 12~24h under the conditions of 25~33 DEG C, sprayed during germination every 2h Drench mixed aqueous solution 1 time, 50~80mmol/L sodium chloride and 5~10mmol/L calcium chloride are contained in mixed aqueous solution, to bud length 1 ~10mm, the sprouted broad bean rich in γ-aminobutyric acid is made；3）Defibrination compounds：Obtained sprouted broad bean is removed slag obtained soya-bean milk by known method defibrination；Add 2~6%（W/V）Lactose, Heated by known method and sterilize and adjust pH as 6.0~7.5；4）Lactic fermentation：The Lactobacillus plantarum of highly producing gamma-aminobutyric acid presses known actication of culture method and activates 2 obtained two levels Seed liquor；By 3~6%（V/V）Secondary seed solution is inoculated with soya-bean milk, fermentation is made in the 4~12h that fermented under the conditions of 33~40 DEG C Soya-bean milk；5）Allocate homogeneous, sterilization filling：By fermented soybean milk by known allotment homogeneous and the processing of sterilization filling method, it is made and is rich in The fermented soybean milk of γ-aminobutyric acid.
- 3. a kind of production method of fermented soybean milk rich in γ-aminobutyric acid according to claim 1 or 2, its feature exist In：Alpha-aminobutyric acid content is 15~30 mg/100mL in obtained fermented soybean milk.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110881528A (en) * | 2018-09-11 | 2020-03-17 | 南京农业大学 | Production technology of highland barley bean pollen rich in gamma-aminobutyric acid and ferulic acid |
| CN112715798A (en) * | 2019-10-14 | 2021-04-30 | 南京农业大学 | Method for increasing folic acid content in malt juice beverage |
| CN113519784A (en) * | 2021-07-01 | 2021-10-22 | 上海紫泉饮料工业有限公司 | Kidney bean plant protein beverage rich in gamma-aminobutyric acid and preparation method thereof |
| CN114009690A (en) * | 2021-09-30 | 2022-02-08 | 上海朵眠生物科技有限公司 | Preparation method of fermented soybean milk containing gamma-aminobutyric acid and prepared fermented product |
| CN114403353A (en) * | 2022-02-11 | 2022-04-29 | 青海汉和生物科技股份有限公司 | Process method of germinated black highland barley powder rich in gamma-aminobutyric acid |
| CN114698771A (en) * | 2022-03-02 | 2022-07-05 | 安徽省蒸谷米食品科技有限公司 | Processing technology of gamma aminobutyric acid-rich parboiled rice |
| CN116210861A (en) * | 2021-12-06 | 2023-06-06 | 内蒙古伊利实业集团股份有限公司 | Soybean milk containing gamma-aminobutyric acid and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110881528A (en) * | 2018-09-11 | 2020-03-17 | 南京农业大学 | Production technology of highland barley bean pollen rich in gamma-aminobutyric acid and ferulic acid |
| CN112715798A (en) * | 2019-10-14 | 2021-04-30 | 南京农业大学 | Method for increasing folic acid content in malt juice beverage |
| CN113519784A (en) * | 2021-07-01 | 2021-10-22 | 上海紫泉饮料工业有限公司 | Kidney bean plant protein beverage rich in gamma-aminobutyric acid and preparation method thereof |
| CN114009690A (en) * | 2021-09-30 | 2022-02-08 | 上海朵眠生物科技有限公司 | Preparation method of fermented soybean milk containing gamma-aminobutyric acid and prepared fermented product |
| CN116210861A (en) * | 2021-12-06 | 2023-06-06 | 内蒙古伊利实业集团股份有限公司 | Soybean milk containing gamma-aminobutyric acid and preparation method thereof |
| CN114403353A (en) * | 2022-02-11 | 2022-04-29 | 青海汉和生物科技股份有限公司 | Process method of germinated black highland barley powder rich in gamma-aminobutyric acid |
| CN114698771A (en) * | 2022-03-02 | 2022-07-05 | 安徽省蒸谷米食品科技有限公司 | Processing technology of gamma aminobutyric acid-rich parboiled rice |
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Application publication date: 20171201 |