CN102919376B - High gamma-aminobutyric acid germ bean milk and production process thereof - Google Patents
High gamma-aminobutyric acid germ bean milk and production process thereof Download PDFInfo
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- CN102919376B CN102919376B CN201210464187.1A CN201210464187A CN102919376B CN 102919376 B CN102919376 B CN 102919376B CN 201210464187 A CN201210464187 A CN 201210464187A CN 102919376 B CN102919376 B CN 102919376B
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Abstract
The invention relates to high gamma-aminobutyric acid germ bean milk and a production process of the high gamma-aminobutyric acid germ bean milk, belonging to the technical field of deeply processing of food. The high gamma-aminobutyric acid germ bean milk is characterized by being prepared by the steps of: taking sprouted broad bean prepared from bean grains in a sprouting manner by low-oxygen compelling, compelling at low temperature, homogenating at low temperature, carrying out complex enzymolysis, siruping and homogenizing, sterilizing and filling. According to the production process, endogenous enzyme released by the sprouted broad bean in a low-temperature homogenating process is fully utilized, and an enzymatic condition is controlled at the same time, so that the purpose of gathering gamma-aminobutyric acid is achieved. The high gamma-aminobutyric acid germ bean milk has the characteristics of wide material source, simple production process, industrialization and the like. The obtained product has high nutritive value and mellow taste, and the content of the gamma-aminobutyric acid is 20-35mg/100mL, and the obtained product is ideal health-care drink.
Description
One, technical field
The present invention relates to a kind of high gamma-aminobutyric acid plumule soya-bean milk and production technology thereof, belong to technical field of food deep processing.
Two, technical background
GABA (γ-Aminobutyric acid, GABA) be in mammal brain, marrow, to suppress neural mediator, have hypotensive, anticonvulsion, ease pain, improve brain function, stable neural, promote long-term memory, promote growth hormone secretion, the function such as activation liver kidney, in 2009, by health ministry, be approved as new resource food.The exploitation of therefore, being rich in the food of GABA has become focus.Research shows, plant in thermostimulation, anoxic, damage to plants caused by sudden drop in temperature, under the adverse environmental factor such as mechanical stimulus and salt stress, GABA content and relevant enzyme vigor can increase rapidly and significantly.Bean material source is wide, is developed to the food that is rich in GABA, and this is conducive to promote the nutrition and health care value of bean food.
At present, the bean products report of GABA is rich in domestic existing research and development, patent (publication number CN101347212A, open day on January 21st, 2009) " soybean processed foods of high-content gamma-amino acid and manufacture method " disclosed, by high temperature stress enrichment GABA, these are different from the effect of low temperature stress enrichment GABA, thereby its Related product production technology also exists substantial differences; Patent (publication number CN1988812A, open day on June 27th, 2007) discloses " for improving the method for soya seeds alpha-aminobutyric acid content ", directly soya seeds is soaked by freezing and processes with enrichment GABA with thawing.This germinates beans seed rear, different through the production technology of low temperature stress enrichment GABA again from the present invention.Soya seeds is through germination treatment, and the functional materials content such as polypeptide wherein, isoflavones significantly increase, and the ANFs such as phytic acid, protease inhibitors, erythrocyte agglutination element have degraded in various degree.
Patent (publication number CN1287475A, open day March 14 calendar year 2001) " production method of soya-bean milk " disclosed, patent (publication number CN102655758A, open day on September 15th, 2012) " manufacturing the method and system of full soya-bean milk " disclosed, patent (publication number CN102106394A, open day on June 29th, 2011) " a kind of production technology of black soymilk " disclosed, patent (publication number CN102524412A, open day on July 4th, 2012) " a kind of preparation method of broad bean milk beverage " and patent (publication number CN101828601A are disclosed, open day on September 15th, 2010) " a kind of production method of pure soya-bean milk " disclosed.These patents relevant to soya-bean milk only provide common Soymilk Processing.
Three, summary of the invention
Technical problem:
The object of the invention is to provide a kind of high gamma-aminobutyric acid plumule soya-bean milk and production technology thereof, adopts environment stress technique enrichment GABA in bean sprouts, as raw material, produces plumule soya-bean milk, can promote the health care of soya-bean milk products.
Technical scheme:
Technical solution of the present invention is summarized as follows: the sprouted broad bean that the beans seed of take obtains through Hypoxia Stress germination is in short-term raw material, again through low temperature stress, low-temperature homogenate, composite enzymolysis, mashing off homogeneous and sterilization filling, make be of high nutritive value, high gamma-aminobutyric acid plumule soya-bean milk that mouthfeel is mellow.
Above-mentioned a kind of high gamma-aminobutyric acid plumule soya-bean milk and production technology thereof, comprising:
(1) raw material is selected: select results then, germination percentage makes germination raw material at more than 95% beans seed; Beans seed can be made raw material with soybean kernel, can be also raw material with beans seeds such as mung bean or broad bean or red bean or peas, and kind is not limit.
(2) hypoxemia germinates: beans seed is sterilized after 30min in 1% clorox, is placed in 10 times of amounts (V/W) water, in 30 ℃, soaks 6h; Then be placed in 15～25 times of amounts (V/W) water, in 25～35 ℃ of ventilation lucifuges germination 12～48h, throughput is 0.7～1.2L/min.
(3) low temperature stress: sprouted broad bean is coerced to 5～25h under-20 ℃～10 ℃ low temperature.
(4) low-temperature homogenate: will coerce rear sprouted broad bean and remove after surface moisture, and add 0～10 ℃ of water of 5～10 times of amounts (V/W) to carry out homogenate.
(5) composite enzymolysis: make beans homogenate by the method for mechanical homogenate, the sprouted broad bean homogenate alive with having enzyme mixes in 1: 1～1: 3 (VN) ratio, carries out enzyme digestion reaction 1～3h in 30 ℃～50 ℃.
(6) mashing off homogeneous, sterilization filling: raw soya-bean milk liquid is crossed 120 mesh sieves after 100 ℃ of boilings, makes soya-bean milk magma; Adopt second homogenate method, homogenizing temperature is 60 ℃, and homogenization pressure is 25MPa for the first time, and the soya-bean milk after homogeneous is sent to high temperature sterilization machine, and in 121 ℃ of sterilizing 15min, the homogenizer that enters pressure after cooling and be 20MPa carries out homogeneous for the second time; Cooled soya-bean milk is carried out to sterile filling, make high GABA plumule soya-bean milk finished product.
Beneficial effect:
Compared with prior art, a kind of high gamma-aminobutyric acid plumule soya-bean milk of the present invention and production technology thereof have the following advantages:
(1) the present invention adopts low temperature stress and low-temperature homogenate technique, make composite common beans homogenate after low-temperature homogenate liquid, take full advantage of the endogenous enzymes that in low-temperature homogenate process, sprouted broad bean discharges, regulate and control enzymatic reaction condition simultaneously, obtain high gamma-aminobutyric acid plumule soya-bean milk.
(2) in the present invention, beans seed, after germination treatment, has improved its nutritional labeling, has reduced ANFs, has improved the digest and absorb rate of human body to soybean.
(3) products obtained therefrom of the present invention is nutritious, mouthfeel is mellow, alpha-aminobutyric acid content is high, is a kind of desirable health drink.
Four, the specific embodiment
Embodiment 1
Soybean kernel is sterilized after 30min in 1% clorox, is placed in 10 times of amounts (V/W) water, in 30 ℃, soaks 6h; Soybean after immersion is placed in 15 times of amounts (V/W) water, and in 30 ℃ of ventilation lucifuge germination 24h, throughput is 1.0L/min; Germinated soybean, in-5 ℃ of low temperature stress 12h, is then removed to surface moisture, add 5 ℃ of drinking water of 8 times of amounts (V/W) to carry out homogenate; Low-temperature homogenate liquid and soybean homogenate are mixed in 1: 2 (V/V) ratio, in 40 ℃ of enzyme digestion reaction 2h; Raw soya-bean milk liquid boils rear mistake 120 mesh sieves, makes soya-bean milk magma; Soya-bean milk is carried out to homogeneous processing, and homogenizing temperature is 60 ℃, and homogenization pressure is 25MPa, and the soya-bean milk after homogeneous is sent to high temperature sterilization machine, and in 121 ℃ of sterilizing 15min, the homogenizer that enters pressure after cooling and be 20MPa carries out second homogenate; Cooled soya-bean milk is carried out to sterile filling, make.
Embodiment 2
Broadbean seed sterilization immersion process is with embodiment 1; Be placed in 10 times of amounts (V/W) water, in 35 ℃ of ventilation lucifuge germination 12h, throughput is 0.8L/min; Germination broad bean is coerced to 5h in-20 ℃; Remove after germination broad bean surface moisture, adding 5 times of amounts (V/W) temperature is that the drinking water of 0 ℃ carries out homogenate; Low-temperature homogenate liquid and broad bean homogenate are mixed in 1: 1 (V/V) ratio, in 30 ℃ of enzyme digestion reaction 1h; Mashing off homogeneous and sterilization filling method, with embodiment 1, make.
Embodiment 3
Red bean seed sterilization immersion process is with embodiment 1; Be placed in 12 times of amounts (V/W) water, in 28 ℃ of ventilation lucifuge germination 36h, throughput is 1.2L/min; Germination red bean is coerced to 25h in 10 ℃; Remove after germination red bean surface moisture, adding 10 times of amounts (V/W) temperature is that the drinking water of 10 ℃ carries out homogenate; Low-temperature homogenate liquid and red bean homogenate are mixed in 1: 1 (V/V) ratio, in 50 ℃, carry out enzyme digestion reaction 3h; Mashing off homogeneous and sterilization filling method, with embodiment 1, make.
Embodiment 4
Pea seed sterilization immersion process is with embodiment 1; Be placed in 15 times of amounts (V/W) water, in 35 ℃ of ventilation lucifuge germination 12h, throughput is 0.9L/min; Germination pea is coerced to 18h in-10 ℃; Coerce rear germination pea and remove after surface moisture, adding 5 times of amounts (V/W) temperature is that the drinking water of 0 ℃ carries out homogenate; Low-temperature homogenate liquid and pea homogenate are mixed in 1: 1 (V/V) ratio, in 30 ℃, carry out enzyme digestion reaction 1h; Mashing off homogeneous and sterilization filling method, with embodiment 1, make.
Embodiment 5
Mung bean seed sterilization immersion process is with embodiment 1; Be placed in 8 times of amounts (V/W) water, in 30 ℃ of ventilation lucifuge germination 36h, throughput is 0.9L/min; Germination mung bean is coerced to 20h in-5 ℃; Coerce rear germination mung bean and remove after surface moisture, adding 10 times of amounts (V/W) temperature is that the drinking water of 5 ℃ carries out homogenate; Low-temperature homogenate liquid and mung bean homogenate are mixed in 1: 3 (V/V) ratio, in 35 ℃, carry out enzyme digestion reaction 1.5h; Mashing off homogeneous and sterilization filling method, with embodiment 1, make.
More than describe embodiments of the present invention in detail, but this example of just lifting for the ease of understanding should not be considered to be limitation of the scope of the invention.Equally; any person of ordinary skill in the field all can be according to the description of technical scheme of the present invention and preferred embodiment thereof; make various possible being equal to and change or replace, but all these changes or replacement all should belong to the protection domain of claim of the present invention.
Claims (4)
1. a high gamma-aminobutyric acid plumule Soymilk Processing, it is characterized in that: the sprouted broad bean that the beans seed of take obtains through Hypoxia Stress germination is in short-term raw material, through low temperature stress, low-temperature homogenate, composite enzymolysis, mashing off homogeneous and sterilization filling, make again, specifically comprise the following steps:
(1) hypoxemia germinates: beans seed, by known method, is containing Cl
-the aqueous solution in after sterilization and band water soaking process, be placed in 15～25 times of amounts (V/W) water, in 25～35 ℃ of ventilation lucifuges germination 12～48h;
(2) low temperature stress: sprouted broad bean is coerced to 5～25h under-20 ℃～10 ℃ low temperature;
(3) low-temperature homogenate: will coerce rear sprouted broad bean and remove after surface moisture, adding 5～10 times of amounts (V/W) temperature is that the drinking water of 0～10 ℃ carries out homogenate;
(4) composite enzymolysis: make beans homogenate by known mechanical homogenate method, the homogenate sprouted broad bean homogenate alive with having enzyme mixes in proportion, and carries out enzyme digestion reaction 1～3h in 30 ℃～50 ℃;
(5) mashing off homogeneous, sterilization filling: the homogenate after enzyme digestion reaction is processed according to known mashing off homogeneous, sterilization filling method, made.
2. according to claim 1, a kind of high gamma-aminobutyric acid plumule Soymilk Processing, is characterized in that: Hypoxia Stress is controlled at throughput within the scope of 0.7～1.2L/min by known method while germinateing; Thereafter carry out low temperature stress, temperature is-20 ℃～10 ℃.
3. according to claim 1, a kind of high gamma-aminobutyric acid plumule Soymilk Processing, is characterized in that: low-temperature homogenate liquid and beans homogenate mixed proportion are 1: 1～1: 3 (V/V).
4. according to described in claim 1-3 any one, when a kind of high gamma-aminobutyric acid plumule soya-bean milk is produced, can make raw material with soybean kernel, also can be raw material with mung bean or broad bean or red bean or pea seed, products obtained therefrom is of high nutritive value, mouthfeel is mellow, and alpha-aminobutyric acid content reaches 20～35mg/100mL.
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| CN107410508A (en) * | 2017-05-05 | 2017-12-01 | 扬州大学 | A kind of production method of the fermented soybean milk rich in gamma aminobutyric acid |
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| CN104432375A (en) * | 2014-11-27 | 2015-03-25 | 苏州嘉禧萝生物科技有限公司 | Preparation method of food beverage containing GABA (gamma amino acid-butyric acid) |
| CN104432354B (en) * | 2014-12-19 | 2017-01-11 | 华南农业大学 | Preparation method of litchi juice rich in gamma-aminobutyric acid and litchi juice |
| CN106172810A (en) * | 2016-06-29 | 2016-12-07 | 南京农业大学 | A kind of Semen sojae atricolor rich in gamma aminobutyric acid mixes soyabean milk and production technology thereof |
| CN106070659B (en) * | 2016-06-29 | 2019-07-09 | 南京农业大学 | A kind of soya-bean milk and its production technology rich in inositol -3- phosphoric acid |
| CN107836526A (en) * | 2016-09-19 | 2018-03-27 | 南京农业大学 | A kind of feature sand rice compound bean milk and its production technology |
| CN110881528A (en) * | 2018-09-11 | 2020-03-17 | 南京农业大学 | Production technology of highland barley bean pollen rich in gamma-aminobutyric acid and ferulic acid |
| CN112806534A (en) * | 2021-01-18 | 2021-05-18 | 安徽省农业科学院农产品加工研究所 | Functional whole grain mixed soybean milk and production method thereof |
| CN114376134A (en) * | 2022-01-24 | 2022-04-22 | 河南农业大学 | Flax germ milk rich in secoisolariciresinol and isoflavone and production method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107410508A (en) * | 2017-05-05 | 2017-12-01 | 扬州大学 | A kind of production method of the fermented soybean milk rich in gamma aminobutyric acid |
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