CN114376134A - Flax germ milk rich in secoisolariciresinol and isoflavone and production method thereof - Google Patents
Flax germ milk rich in secoisolariciresinol and isoflavone and production method thereof Download PDFInfo
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- CN114376134A CN114376134A CN202210081562.8A CN202210081562A CN114376134A CN 114376134 A CN114376134 A CN 114376134A CN 202210081562 A CN202210081562 A CN 202210081562A CN 114376134 A CN114376134 A CN 114376134A
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- flax
- isoflavone
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- secoisolariciresinol
- seeds
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/60—Drinks from legumes, e.g. lupine drinks
- A23L11/65—Soy drinks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
- A23L11/70—Germinated pulse products, e.g. from soy bean sprouts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Dairy Products (AREA)
Abstract
The invention relates to a flax germ milk rich in secoisolariciresinol diglucoside and isoflavone and a production method thereof, belonging to the technical field of food processing. The contents of SECO and isoflavone in the flax germ milk produced by the invention are respectively 10-25 mg/100 mL and 10-35 mg/100 mL, and the flax germ milk can obviously regulate female hormone secretion and improve the health condition of girls through the synergistic effect of SECO and isoflavone, and is a high-quality functional beverage.
Description
Technical Field
The invention relates to a flax germ milk rich in secoisolariciresinol and isoflavone and a production method thereof, belonging to the technical field of beverage processing and also belonging to the field of functional food production.
Technical Field
The linseed is rich in protein, fat, dietary fiber and a plurality of micronutrients, and also contains biological active ingredients such as alpha-linolenic acid, lignan, flavone and the like. Flax lignans are phytoestrogens with antioxidant activity, and a large number of researches show that the flax lignans have the effects of resisting oxidation, tumors, viruses and fungi, resisting osteoporosis, reducing serum cholesterol, enhancing autoimmunity, delaying aging, protecting cardiovascular and the like, and have very important significance for human health. The main existing form of the lignan in the flax seeds is ring-opening iso-lariciresinol diglucoside, and the lignan is partially converted into an aglycon form, namely ring-opening iso-lariciresinol (SECO) in the germination process, and the latter has stronger oxidation resistance and is more beneficial to absorption and utilization of a human body.
In addition, isoflavone is a natural nutritional factor with various important physiological activities, is a pure natural phytoestrogen, has physiological effects of resisting cancer, regulating and controlling immune response and the like on a human body, and can also prevent osteoporosis and slow down female climacteric syndrome. After the flax seeds germinate in a specific environment, endogenous enzymes are activated, the phenylpropanoid metabolic pathway is enhanced, a large amount of isoflavones are enriched, the existing forms of the isoflavones are richer, the content of free isoflavone aglycones is increased, and the free isoflavone aglycones can be directly absorbed by the intestinal wall. The invention adopts CaCl2Soaking seeds in the aqueous solution, applying pyridoxal phosphate and L-glutamic acid under the stress of hypoxia in the germination process to obtain germinated flax seeds rich in SECO and isoflavone, and then carrying out research and development on flax germ milk.
There are roughly three types of existing flaxseed beverages: firstly, flaxseeds (kernels) are used as raw materials, the protein effect is highlighted, for example, a patent (publication No. CN111406857A, published 2020, 7 and 14 days) discloses a flaxseed kernel protein beverage and a preparation method thereof, and a patent (publication No. CN106509561A, published 2017, 3 and 22 days) discloses a flaxseed milk seed beverage and a preparation method thereof, wherein the flaxseeds are soaked, ground, filtered and supplemented with additives; secondly, the linseed oil is taken as a raw material, for example, the patent (publication No. CN113040302A, published 2021, 6 months and 29 days) discloses 'a natural health drink rich in omega-3 fatty acid and a preparation method thereof', and the linseed oil is taken as a raw material and is prepared by being assisted by an additive; thirdly, the linseed powder is used as a raw material, for example, "a linseed plant beverage and a preparation method thereof" is disclosed in a patent (publication No. CN106509561A, publication No. 2018, 7 and 24), and "a linseed beverage" is disclosed in a patent (publication No. CN104146313A, publication No. 2014, 11 and 19) and is prepared by mixing the linseed powder and other additives with water. The invention relates to flax germ milk rich in SECO and isoflavone and a production method thereof, belonging to the food processing field.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide the flax germ milk rich in secoisolariciresinol-SECO and isoflavone and the production method thereof, and the flax germ milk rich in SECO and isoflavone is enriched by adopting a specific biological regulation and conversion technology, so that the flax germ milk rich in SECO and isoflavone is developed, and the health-care function of the flax dairy product is greatly improved.
In order to solve the technical problems, the invention adopts the following technical scheme:
a method for producing flax germ milk rich in open-loop iso-lariciresinol and isoflavone comprises the following steps of adding a specific enzyme activator into flax and soybeans under hypoxia stress, culturing and germinating the flax and the soybeans, enriching the open-loop iso-lariciresinol and the isoflavone, taking the germinated flax and soybeans as raw materials, pulping, adding skim milk powder, xylitol, sodium erythorbate, monoglyceride, sucrose fatty acid ester, sodium carboxymethylcellulose and flaxseed gum, uniformly mixing, homogenizing, degassing, sterilizing and aseptically filling to obtain the flax germ soybean milk rich in the open-loop iso-lariciresinol and the isoflavone, wherein the method specifically comprises the following steps:
(1) screening and sterilizing: selecting full-grained flax seeds without diseases and insect pests and with high germination rate, soaking the flax seeds in 0.5% (w/v) sodium hypochlorite aqueous solution for 15 min, and washing the flax seeds to be neutral by clear water;
(2) hypoxia stress combined with enzyme activator germination: placing the disinfected flax seeds in 2.0-8.0 mmol/L CaCl2Soaking in the aqueous solution for 4-8 h at 15-30 ℃; then transferring the mixture into a germination tank, adding a mixed aqueous solution of 0.1-2 mmol/L pyridoxal phosphate and 0.2-2 mmol/L L-glutamic acid, andintroducing air into the germination tank at a flow rate of 0.5-2.0L/min, and culturing at 10-30 ℃ in the dark for 12-48 h to obtain flax seeds with the bud length of 1-5 mm;
(3) preparing primary pulp: cleaning the flax seeds, mixing the cleaned flax seeds with purified water according to a material-liquid ratio (w/w) of 1: 2-1: 9, setting the rotation speed of a colloid mill to be 3000-5000 r/min, grinding the flax seeds into pulp for 8-15 min, and filtering and removing slag by using filter cloth of 90-130 meshes to prepare fresh flax germ protoplasm rich in SECO and isoflavone;
(4) boiling and blending: boiling the primary pulp of the flax germs at 85-95 ℃ for 30-45 min, filtering, adding 8-20% of skim milk powder, 3-6% of xylitol, 0.5-2.0% of sodium erythorbate, 0.05-0.15% of monoglyceride, 0.05-0.12% of sucrose fatty acid ester, 0.10-0.20% of sodium carboxymethylcellulose and 0.05-0.15% of flaxseed gum according to the material-to-liquid ratio (w/w), and uniformly mixing the materials;
(5) homogenizing and degassing: homogenizing the blended slurry for the first time under 15-20 MPa, then increasing the pressure to 30-40 MPa, and homogenizing for the second time at the temperature of 50-65 ℃; degassing at 65-75 ℃ and under the vacuum degree of 0.05-0.10 MPa;
(6) and (3) sterilization and filling: and (3) carrying out high-temperature instantaneous sterilization on the flax germ milk for 3-10 s at the high temperature of 121-135 ℃, cooling to room temperature by using a plate heat exchanger, and carrying out aseptic filling to obtain the flax germ milk rich in the secoisolariciresinol diglucoside and isoflavone.
Compared with the prior art, the invention has the following beneficial effects:
(1) after the flax seeds are subjected to germination treatment, the nutritional ingredients are improved, the content of anti-nutritional factors is reduced, and the digestion, absorption and utilization rate of a human body is improved;
(2) the invention adopts unique biological regulation and conversion technology, the prepared flax germ milk is rich in SECO and isoflavone, can realize synergistic interaction, regulate female hormone secretion and relieve female climacteric syndrome;
(3) the production method is green and environment-friendly, is not limited by seasons, climates and regions, and provides an industrial technology for producing the flax germ milk rich in SECO and isoflavone.
Drawings
FIG. 1 shows CaCl2Influence of concentration on the SECO content of the flax sprouts;
FIG. 2 shows CaCl2Influence of concentration on isoflavone content of flax sprouts;
FIG. 3 is a graph of the effect of L-Glu concentration on SECO content of flax sprouts;
FIG. 4 is the effect of L-Glu concentration on the isoflavone content of flax sprouts;
FIG. 5 is a graph of the effect of pyridoxal phosphate (PLP) concentration on SECO content of flax sprouts;
FIG. 6 is a graph of the effect of pyridoxal phosphate (PLP) concentration on the isoflavone content of flax sprouts.
Detailed Description
The present invention will be further described with reference to the following examples. It is to be understood that the following examples are illustrative only and are not intended to limit the scope of the invention, which is to be given numerous insubstantial modifications and adaptations by those skilled in the art based on the teachings set forth above.
Example 1
The production method of the flax germ milk rich in secoisolariciresinol diglucoside and isoflavone comprises the following steps:
selecting full-grained flax seeds without diseases and insect pests and with high germination rate, soaking the flax seeds in 0.5% (v/v) sodium hypochlorite aqueous solution for 15 min, and washing the flax seeds to be neutral by clear water; then placing the mixture in 4.0 mmol/L CaCl2Soaking in water solution at 20 deg.C for 6 hr, transferring into germination tank, adding mixed water solution of 1.5 mmol/L pyridoxal phosphate and 1.0 mmol/L L-glutamic acid, introducing air into the germination tank at flow rate of 1.0L/min, and culturing at 20 deg.C in dark for 36 hr; selecting flax seeds with the embryo length of 5 mm, cleaning, mixing with purified water according to a material-liquid ratio (w/v) of 1:3, setting the rotation speed of a colloid mill to 4500 r/min, grinding for 10 min, and filtering with 120-mesh filter cloth to remove residues to obtain fresh flax embryo raw stock; decocting the raw pulp at 90 deg.C for 40 min, filtering, adding 15% of skimmed milk powder, 4% of xylitol, 1.0% of sodium erythorbate, 0.08% of monoglyceride, 0.1% of sucrose fatty acid ester, 0.12% of sodium carboxymethylcellulose and 0.1% of flaxseed gum according to the material-to-liquid ratio (w/v), and mixing; homogenizing the blended slurry for the first time under 15-20 MPa, then increasing the pressure to 30-40 MPa, and feedingCarrying out secondary homogenization at the temperature of 50-65 ℃; degassing at 65-75 ℃ and under the vacuum degree of 0.05-0.10 MPa; and (3) carrying out high-temperature instantaneous sterilization on the flax germ milk for 3-10 s at the high temperature of 121-135 ℃, cooling to room temperature by using a plate heat exchanger, and carrying out aseptic filling to obtain the products with the SECO and isoflavone contents of 20 mg/100 mL and 28 mg/100 mL respectively.
Example 2
The production method of the flax germ milk rich in secoisolariciresinol diglucoside and isoflavone comprises the following steps:
the raw material screening and sterilizing method is the same as that in example 1, the raw material is selected and sterilized and then placed in 2.0 mmol/L CaCl2Soaking in water solution at 15 deg.C for 8 hr, transferring into germination tank, adding mixed water solution of 1.0 mmol/L pyridoxal phosphate and 0.8 mmol/L L-glutamic acid, introducing air into the germination tank at flow rate of 1.5L/min, and culturing at 10 deg.C in dark for 48 hr; selecting flax seeds with the germ length of 4 mm, cleaning, mixing with purified water according to the material-liquid ratio (w/v) of 1:4, grinding, filtering and boiling the pulp as in example 1, adding 18% of skim milk powder, 4% of xylitol, 1.0% of sodium erythorbate, 0.10% of monoglyceride, 0.08% of sucrose fatty acid ester, 0.11% of sodium carboxymethylcellulose and 0.12% of flaxseed gum according to the material-liquid ratio (w/v), and uniformly mixing; the method of homogenization, degassing, sterilization and filling is the same as that of example 1. The prepared product has SECO and isoflavone contents of 18 mg/100 mL and 25 mg/100 mL respectively.
Example 3
The production method of the flax germ milk rich in secoisolariciresinol diglucoside and isoflavone comprises the following steps:
the raw material screening and sterilizing method is the same as that in example 1, the raw material is selected and sterilized and then placed in 6.0 mmol/L CaCl2Soaking in water solution at 30 deg.C for 4 hr, transferring into germination tank, adding mixed water solution of pyridoxal phosphate 0.3 mmol/L and glutamic acid 1.2 mmol/L L, introducing air into the germination tank at flow rate of 0.8L/min, and culturing at 25 deg.C in dark for 24 hr; selecting flax seeds with the germ length of 3 mm, cleaning, mixing with purified water according to the material-liquid ratio (w/v) of 1:5, preparing primary pulp, boiling the pulp, blending, homogenizing, degassing, sterilizing and filling by the same method as the example 1. The prepared product has SECO and isoflavone contents of 15 mg/100 mL and 20 mg/100 mL respectively.
As shown in FIGS. 1 and 2, respectively, CaCl2The concentration has influence on SECO and isoflavone content of flax sprout, and the culture condition is that the selected flax seeds are soaked in 0.5% (v/v) NaClO water solution for sterilization for 15 min, and then placed in CaCl series after cleaning2Soaking in distilled water with concentration, germinating for 24 hr, and selecting fresh flax sprout for determination.
The effect of L-Glu concentration on SECO and isoflavone content of flax sprouts, as shown in FIGS. 3 and 4, respectively, Note: the culture conditions are that the selected flax seeds are soaked in 0.5% (v/v) NaClO aqueous solution for disinfection for 15 min, and then are sprayed under the concentration of series L-glutamic acid for germination for 12h, 24h and 36 h after being cleaned and soaked, and fresh flax sprouts are selected for determination.
The effect of PLP concentration on flax sprouts SECO and isoflavone content, respectively, is shown in fig. 4 and 6, noting that: the culture conditions comprise that the selected flax seeds are soaked in 0.5% (v/v) NaClO aqueous solution for disinfection for 15 min, and then are sprayed under a series of PLP concentrations for germination for 12h, 24h and 36 h after being cleaned and soaked, and fresh flax sprouts are selected for determination.
The invention adopts specific biological regulation and conversion technology to enrich SECO and isoflavone in the germinated flax, and then develops a flax germ milk product rich in SECO and isoflavone, which can effectively regulate the secretion of female hormone, reduce the incidence rates of coronary heart disease, genital atrophy, osteoporosis and the like in perimenopausal women and relieve female climacteric syndrome.
The embodiments of the present invention have been described in detail above, but this is only an example for easy understanding and should not be construed as limiting the scope of the present invention. Also, various equivalent changes or substitutions are possible for those skilled in the art according to the technical solution of the present invention and the description of the preferred embodiment thereof, but all such changes or substitutions shall fall within the protection scope of the claims of the present invention.
Claims (10)
1. A production method of flax germ milk rich in secoisolariciresinol diglucoside and isoflavone is characterized in that flax seeds are germinated by adding a specific enzyme activator under hypoxia stress, then the secoisolariciresinol diglucoside SECO and isoflavone are enriched, the germinated flax seeds are taken as raw materials for pulping, and then skim milk powder, xylitol, sodium erythorbate, monoglyceride, sucrose fatty acid ester, sodium carboxymethylcellulose and flaxseed gum are added, and after uniform mixing, the flax germ milk is prepared by homogenizing, degassing, sterilizing and sterile filling, and the method specifically comprises the following steps:
(1) screening and sterilizing: selecting full-grained flax seeds without diseases and insect pests and with high germination rate, soaking and disinfecting the flax seeds with a sodium hypochlorite aqueous solution, and washing the flax seeds with clear water to be neutral;
(2) hypoxia stress combined with enzyme activator germination: placing the disinfected flax seeds in 2.0-8.0 mmol/L CaCl2Soaking in the aqueous solution for 4-8 h at 15-30 ℃; then transferring the flax seeds into a germination tank, adding a mixed aqueous solution of 0.1-2 mmol/L pyridoxal phosphate and 0.2-2 mmol/L L-glutamic acid, introducing air into the germination tank at a flow rate of 0.5-2.0L/min, and culturing at 10-30 ℃ in the dark for 12-48 h to obtain germinated flax seeds;
(3) preparing primary pulp: cleaning the germinated flax seeds obtained in the step (2), mixing with purified water, then placing into a colloid mill for grinding, and then filtering and removing slag to obtain fresh flax germ protoplasm rich in open-loop iso-laric phenol SECO and isoflavone;
(4) boiling and blending: boiling and filtering the primary pulp of the flax germs, then adding the skim milk powder, the xylitol, the sodium erythorbate, the monoglyceride, the sucrose fatty acid ester, the sodium carboxymethylcellulose and the flaxseed gum, and uniformly mixing the materials to obtain a serous fluid;
(5) homogenizing and degassing: homogenizing the slurry obtained in the step (4) for two times and then degassing;
(6) and (3) sterilization and filling: sterilizing flax germ milk, cooling to room temperature with plate heat exchanger, and aseptically packaging.
2. The method for producing secoisolariciresinol and isoflavone-rich flax germ milk according to claim 1, wherein: in the step (1), the concentration of the sodium hypochlorite aqueous solution is 0.5% (v/v), and the soaking time is 15 min.
3. The method for producing secoisolariciresinol and isoflavone-rich flax germ milk according to claim 1, wherein: and (3) obtaining the flax seeds with the germinated flax seeds of 1-5 mm in the step (2).
4. The method for producing secoisolariciresinol and isoflavone-rich flax germ milk according to claim 1, wherein: cleaning the germinated flax seeds in the step (3), and then mixing the cleaned flax seeds with purified water in a mass ratio of 1: 2-1: 9; and during grinding, setting the rotating speed of a colloid mill to be 3000-5000 r/min, setting the grinding time to be 8-15 min, and setting filter cloth used for filtering to be 90-130 meshes.
5. The method for producing secoisolariciresinol and isoflavone-rich flax germ milk according to claim 1, wherein: boiling the primary flax germ pulp at 85-95 ℃ for 30-45 min in step (4), and adding 8-20% of skim milk powder, 3-6% of xylitol, 0.5-2.0% of sodium erythorbate, 0.05-0.15% of monoglyceride, 0.05-0.12% of sucrose fatty acid ester, 0.10-0.20% of sodium carboxymethylcellulose and 0.05-0.15% of flaxseed gum by mass of the primary flax germ pulp in step (4).
6. The method for producing secoisolariciresinol and isoflavone-rich flax germ milk according to claim 1, wherein: performing primary homogenization under the pressure of 15-20 MPa in the step (5), then increasing the pressure to 30-40 MPa, and performing secondary homogenization at the temperature of 50-65 ℃; degassing at 65-75 ℃ and 0.05-0.10 MPa of vacuum degree.
7. The method for producing secoisolariciresinol and isoflavone-rich flax germ milk according to claim 1, wherein: and (6) carrying out high-temperature instantaneous sterilization on the flax germ milk for 3-10 s at the high temperature of 121-135 ℃.
8. The method for producing secoisolariciresinol and isoflavone-rich flax germ milk according to claim 1, wherein: the flax seeds in the step (1) are brown flax seeds, golden flax seeds or the mixture of the brown flax seeds and the golden flax seeds.
9. Flax germ milk enriched with secoisolariciresinol and isoflavones obtained by the production process according to any one of claims 1 to 8.
10. Flax germ milk enriched with secoisolariciresinol and isoflavones according to claim 9, characterized in that: the content of SECO in the flax germ milk is 10-25 mg/100 mL, and the content of isoflavone is 10-35 mg/100 mL.
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