CN101273769A - Method for preparing germinated brown rice with high-content gamma-aminobutyric acid - Google Patents

Method for preparing germinated brown rice with high-content gamma-aminobutyric acid Download PDF

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Publication number
CN101273769A
CN101273769A CNA2008100251381A CN200810025138A CN101273769A CN 101273769 A CN101273769 A CN 101273769A CN A2008100251381 A CNA2008100251381 A CN A2008100251381A CN 200810025138 A CN200810025138 A CN 200810025138A CN 101273769 A CN101273769 A CN 101273769A
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brown rice
rice
paddy
soak
aminobutyric acid
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CN101273769B (en
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张晖
郭晓娜
王立
孟祥勇
姚惠源
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Jiangnan University
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Jiangnan University
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Abstract

The invention relates to a method for preparing gemmiparous brown rice in which Gamma-aminobutyric acid is rich, belonging to the technical field of fine and further processing of rice; the invention adopts fresh rice of the same year as raw materials which are hulled, paddy-separated and well-chosen, thus obtaining the brown rice; the brown rice is washed, disinfected, soaked, cultivated, sterilized and dried, thus obtaining a finished product; when in cultivation, a buffer solution containing 2 to 5mmol/ L of sodium glutamate, 0.05 to 0.1mmol/L of calcium chloride, 0.01 to 0.05mmol/L of pyridoxal phosphate and with the pH of 5.6 to 5.8 is adopted as cultivation solution, the activity of endogenous glutamate decarboxylase, the glutamic acid and the added sodium glutamate contained in the brown rice are catalyzed and decomposed, thus leading the prepared gemmiparous brown rice to contain the Gamma-aminobutyric acid with high content; the method of the invention has scientific and reasonable design, and the prepared gemmiparous brown rice containing the Gamma-aminobutyric acid as high as 200mg/100g can be steamed and ate directly, and can also be mixed with white rice for steaming and eating with rich nutrition, safety and sanitation.

Description

A kind of preparation method of germinated brown rice with high-content gamma-aminobutyric acid
Technical field
A kind of preparation method of germinated brown rice with high-content gamma-aminobutyric acid belongs to paddy intensive processing technical field.
Background technology
Paddy is one of cereal important on the earth.Update shows that China's rice yield was about 18,650 ten thousand tons in 2007.Paddy is made up of clever shell, pericarp, kind skin, endosperm (comprising aleurone and starchy endosperm) and embryo.In process, paddy promptly is brown rice after the paddy of hulling sloughs clever shell, and brown rice removes pericarp, kind skin, aleurone and embryo and just becomes highed milled rice again through further grinding system.Brown rice not only contains rich in protein, fat, vitamin and mineral matter, but also the rare long-lived factors such as glutathione peroxidase in very high vitamin E, linoleic acid and the food of enrichment physiologically active.Brown rice is organization softening after germinateing, and cooking is convenient, and the nutritive value of sprouted unpolished rice surpasses not sprouted unpolished rice.Sprouted unpolished rice is the multienzyme system that an enzymatic activity is activated, discharges.Particularly brown rice is after germination treatment, and endogenic glutamate decarboxylase activity improves greatly, and the catalysis glutamic acid rotating becomes GABA.GABA (NH 2-CH 2-CH 2-CH 2-COOH) be important inhibiting nerve mediator in mammal, shellfish, insect and some the parasitic worm nervous system.Contain this nonprotein amino acid in the minority plant.Discover that GABA is the active amino acid that plays an important role in the human brain energy metabolism, it has glucose metabolism in the brain of activation, and short acetylcholine is synthetic, falls blood ammonia, and is anticonvulsion, hypotensive, recovers the function of brain cell.The sprouted unpolished rice of the present invention's preparation adopts the phosphate buffered liquid system when soaking, in incubation, add an amount of phosphopyridoxal pyridoxal phosphate, activated the physiologically active of glutamate decarboxylase greatly, make in the sprouted unpolished rice of preparation alpha-aminobutyric acid content up to 200mg/100g.
Summary of the invention
The purpose of this invention is to provide a kind of preparation method of germinated brown rice with high-content gamma-aminobutyric acid, products obtained therefrom is nutritious, and cooking is convenient, and mouthfeel is good, particularly contains a large amount of important physical active material GABAs.
Technical scheme of the present invention: a kind of preparation method of germinated brown rice with high-content gamma-aminobutyric acid, with the fresh paddy when annual output is raw material, through rice huller paddy, the rough separation of paddy and the selected brown rice that obtains, brown rice obtains the finished product germinated brown rice with high-content gamma-aminobutyric acid through washing, sterilization, immersion, cultivation, sterilization and drying again;
(1) rice huller paddy, the rough separation of paddy and selected: adopting the fresh paddy when annual output is raw material, obtain mixture of paddy and husked rice through the rubber roll husker shelling, should guarantee husking yield, reduce broken rice rate again, again through the grain separating machine screening, the flat grain of the selected removal of thickness grader, no embryo grain and prematurity grain obtain particle integrity and full brown rice;
(2) washing: the brown rice of selected mistake washes with running water, and flush away is bonded at the chaff powder and the dust on brown rice surface;
(3) sterilization: the brown rice of wash carries out disinfection to the brown rice surface with the liquor natrii hypochloritis of mass concentration 1% 5min that sterilizes, the generation of minimizing microorganism, and then use sterile water wash brown rice;
(4) soak: the brown rice through sterilization places soak to soak, 15~30 ℃ of soaking temperatures, soak time 6~18h, the mass ratio of brown rice and soak are 1: 2~1: 5, and soak is the clorox that includes 0.05% mass concentration, the phosphate buffer of pH 5.6-5.8;
(5) cultivate: take out the brown rice that soaked and place nutrient solution to germinate, the mass ratio of brown rice and nutrient solution is 1: 2~1: 5, nutrient solution is: the phosphate buffer that includes 2-5mmol/L sodium glutamate, 0.05-0.1mmol/L calcium chloride and 0.01-0.05mmol/L phosphopyridoxal pyridoxal phosphate, pH 5.6-5.8,20~40 ℃ of cultivation temperature, incubation time 8~30h;
(6) sterilization: adopt the mode of hot water treatment and alcohol immersion to sterilize through the sprouted unpolished rice after cultivating, in 90~100 ℃ of hot water, soak 5~10mim earlier, use the alcohol immersion 3~10mim of 70% mass concentration again;
(7) drying: adopt the dry sprouted unpolished rice of fluid bed dryer, oven dry is to controlling moisture about 13%, and the product that obtains is germinated brown rice with high-content gamma-aminobutyric acid.
Beneficial effect of the present invention: the sprouted unpolished rice of the present invention's preparation adopts the phosphate buffered liquid system when soaking, in incubation, add an amount of phosphopyridoxal pyridoxal phosphate, activated the physiologically active of glutamate decarboxylase greatly, the contained glutamic acid and the sodium glutamate of interpolation in the catalytic decomposition brown rice, make the GABA that high-load is arranged in the sprouted unpolished rice of preparation, alpha-aminobutyric acid content is up to 200mg/100g in the sprouted unpolished rice of preparation.
The sprouted unpolished rice that adopts this method to prepare contains abundant GABA, is of high nutritive value, and cooking is convenient, and mouthfeel is good, safety and sanitation.This method provides effective way for the deep development and the utilization of paddy.
The specific embodiment
Embodiment 1
Get 2000g when the fresh paddy of producing per year, send into rubber roll husker and slough rice husk, through grain separating machine screening, selected particle integrity and the full brown rice 1400g of obtaining of thickness grader.The brown rice of selected mistake washes with running water, flush away is bonded at the chaff powder and the dust on brown rice surface, with the liquor natrii hypochloritis of the 1% mass concentration 5min that sterilizes, mainly is that the brown rice surface is carried out disinfection then, reduce the generation of microorganism, then use sterile water wash brown rice again.Brown rice through sterilization places soak to soak, and the mass ratio of brown rice and soak (phosphate buffer of pH5.6 includes 0.05% clorox) is 1: 2~1: 5, soak time 6~18h, 15~30 ℃ of temperature.Taking out the brown rice that soaked places in the ceramic whiteware dish, add the nutrient solution (phosphate buffer of pH 5.6-5.8, include the 2-5mmol/L sodium glutamate, 0.05-0.1mmol/L calcium chloride and 0.01-0.05mmol/L phosphopyridoxal pyridoxal phosphate) germinate, the mass ratio of brown rice and nutrient solution is 1: 2~1: 5,20~40 ℃ of cultivation temperature, incubation time 8~30h.Adopt the mode of hot water treatment and alcohol immersion to sterilize through the brown rice that germinates, in 90~100 ℃ of hot water, soak 5~10mim earlier, use the alcohol immersion 3~10mim of 70% mass concentration again.Adopt the dry brown rice of fluid bed dryer at last, dry to moisture about 13%, alpha-aminobutyric acid content is up to 200mg/100g in the sprouted unpolished rice that obtains.

Claims (1)

1, a kind of preparation method of germinated brown rice with high-content gamma-aminobutyric acid, it is characterized in that: with the fresh paddy when annual output is raw material, through rice huller paddy, the rough separation of paddy and the selected brown rice that obtains, brown rice obtains the finished product germinated brown rice with high-content gamma-aminobutyric acid through washing, sterilization, immersion, cultivation, sterilization and drying again;
(1) rice huller paddy, the rough separation of paddy and selected: adopting the fresh paddy when annual output is raw material, obtain mixture of paddy and husked rice through the rubber roll husker shelling, through the grain separating machine screening, the flat grain of the selected removal of thickness grader, no embryo grain and prematurity grain obtain particle integrity and full brown rice again;
(2) washing: the brown rice of selected mistake washes with running water, and flush away is bonded at the chaff powder and the dust on brown rice surface;
(3) sterilization: the brown rice of wash carries out disinfection to the brown rice surface with the liquor natrii hypochloritis of mass concentration 1% 5min that sterilizes, the generation of minimizing microorganism, and then use sterile water wash brown rice;
(4) soak: the brown rice through sterilization places soak to soak, 15~30 ℃ of soaking temperatures, soak time 6~18h, the mass ratio of brown rice and soak are 1: 2~1: 5, and soak is the clorox that includes 0.05% mass concentration, the phosphate buffer of pH 5.6-5.8;
(5) cultivate: take out the brown rice that soaked and place nutrient solution to germinate, the mass ratio of brown rice and nutrient solution is 1: 2~1: 5, nutrient solution is: the phosphate buffer that includes 2-5mmol/L sodium glutamate, 0.05-0.1mmol/L calcium chloride and 0.01-0.05mmol/L phosphopyridoxal pyridoxal phosphate, pH 5.6-5.8,20~40 ℃ of cultivation temperature, incubation time 8~30h;
(6) sterilization: adopt the mode of hot water treatment and alcohol immersion to sterilize through the sprouted unpolished rice after cultivating, in 90~100 ℃ of hot water, soak 5~10mim earlier, use the alcohol immersion 3~10mim of 70% mass concentration again;
(7) drying: adopt the dry sprouted unpolished rice of fluid bed dryer, oven dry is to controlling moisture 13%.
CN2008100251381A 2008-04-24 2008-04-24 Method for preparing germinated brown rice with high-content gamma-aminobutyric acid Expired - Fee Related CN101273769B (en)

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CN101899382A (en) * 2009-05-31 2010-12-01 陈福生 Preparation method of grain vinegar with high content of gamma-amino butyric acid
CN102132826A (en) * 2011-04-01 2011-07-27 北京市颖欣工贸有限公司 Industrialized production method for instant brown rice
CN101653216B (en) * 2009-09-15 2011-12-14 南京农业大学 Germinated brown rice rich in bioactive peptide
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CN101491282B (en) * 2009-02-20 2012-06-06 上海师范大学 Germination large-germ brown rice tea and production method thereof
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CN102989540A (en) * 2012-11-14 2013-03-27 南京农业大学 Germinated brown rice rich in gamma-aminobutyric acid and capable of being lightly milled and production technology thereof
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CN103919056A (en) * 2014-04-23 2014-07-16 南京农业大学 Method for improving activity of glutamic acid decarboxylase (GAD) of immature brown rice grains
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