CN110812328A - Pet spray dressing and preparation method thereof - Google Patents
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- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
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- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
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- A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
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- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
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Abstract
A pet spray dressing and a preparation method thereof belong to the technical field of pet disinfectants. The method comprises the following steps: adding 0.5-5g of betaine into 100-200g of pure water, stirring and dissolving, adding 0.25-4g of octenidine hydrochloride, and continuously stirring until the betaine is dissolved to obtain a first solution; adding 0.5-10g of polylysine hydrochloride into 400g of pure water of 200-400g, adding 0.06-4.8g of sodium alginate, and continuously stirring until the polylysine hydrochloride is dissolved to obtain a second solution; firstly, 0.5-20g of glycerol is added into 550g of pure water of 300-550g, stirred and dissolved, then 2.4-24g of 2-phenoxyethanol is added, and stirring is continued until the glycerol is dissolved, so as to obtain a third solution; and adding the first solution, the second solution and the third solution into a volumetric flask of 1000ml, adding pure water to a constant volume of 1000ml, standing, filtering and filling to obtain the pet spray dressing. Reducing the occurrence of bacterial infection; will not be resisted by the pet's discomfort.
Description
Technical Field
The invention belongs to the technical field of pet disinfectants, and particularly relates to a pet spray dressing and a preparation method thereof.
Background
The foregoing pet spray dressing may also be referred to as a "pet spray" (hereinafter the same). With the ever-increasing living standard and the diversification of spiritual and cultural lives of people, the housing of pets such as cats and dogs has become a choice for many families or even a fun and spiritual fiduciary life.
As is known: because pet cats, dogs and the like do not have sweat glands, the mode of resisting bacteria and other germs is embodied by the protective film formed by the hairs of the pet cats and dogs and the secretion substances with grease secreted by the skin, the secretion substances exist among the hairs for a long time, dirt is generated, peculiar smell is emitted, and the smell is stronger along with the increase of the air temperature and the humidity, especially in the plum rain season. In addition, bacterial skin diseases of pets are caused by the fact that bacteria easily grow due to the accumulation (i.e., "accumulation") of dirt on the skin. Therefore, it is very important to sterilize pets regularly.
Most of the common pet disinfectants in the prior art are mainly quaternary ammonium salt preparations (benzalkonium chloride, benzalkonium bromide, polyhexamethylene biguanide hydrochloride), chlorine preparations (sodium hypochlorite, sodium dichloroisocyanurate, sodium trichloroisocyanurate and the like) and p-chloro-m-xylenol preparations. If an irritant disinfectant is selected, the pet smells the disinfectant and is exposed to the disinfectant in close proximity, and the disinfectant is easily inhaled into the alveoli, resulting in chronic injury. The cat licks the hair, and the disinfectant components stained on the cat lick the hair possibly. Because cats and dogs do not have the option of being remote from harmful disinfectants, in a sense that cats and dogs are more demanding of disinfectants, typical disinfecting odor-reducing agents are not suitable for use with pets. Therefore, the research on safe, nontoxic and efficient pet disinfectant has positive significance.
Technical information related to pet spray dressings can be seen in published Chinese patent documents, and typically, CN102100723A recommends a "disinfectant special for pets", which discloses the following raw materials and mass percentages thereof: 1-5% of honeysuckle extract, 70-75% of ethanol, 1-3% of glycerol, 0.5-1% of polyoxyethylene ether, 5-10% of polyhexamethylene guanidine and the constant volume of distilled water to 100 (namely 6-22.5% of distilled water); for another example, CN106577802A provides a pet odor-removing disinfectant and a preparation method thereof, which comprises the following raw materials in parts by weight: 2-5 parts of silver nitrate, 3-5 parts of tea polyphenol, 40-80 parts of mint extract, 20-40 parts of sodium dodecyl sulfate, 5-10 parts of polyethylene glycol, 5-10 parts of polyvinyl alcohol, 10-20 parts of polyethylene glycol stearate, 1000 parts of deionized water and a pH value neutral sodium hydroxide solution with mass% concentration of 30%.
The above two patents, which are not limited to the examples, each have the technical effects described in the technical effect column of the specification, but have the following general disadvantages: first, animals such as cats and dogs are relatively lively, prefer to jump up and down or roll around, and thus are prone to form wounds on the skin, so if pet sprays have a physical barrier to wounds (also referred to as "wounds"), bacterial infection can be effectively reduced or even avoided, and the above patents do not have such a function; secondly, since the influence of gram-positive bacteria, gram-negative bacteria and fungi on pets is large and is usually difficult to avoid, and the gram-positive bacteria, gram-negative bacteria and fungi have persistent drug resistance, the problem of avoiding drug resistance of bacteria is difficult to solve in the above patent; thirdly, the antibacterial duration after spraying the pet is relatively short; fourthly, the cleaning and deodorizing effect on the skin surface of the pet is not enough to reach the expectation. In view of the foregoing, the applicant has made advantageous investigations and experiments to develop the technical solutions described below.
Disclosure of Invention
The invention provides a pet spray dressing which is beneficial to exerting excellent cleaning, disinfecting and deodorizing effects on pet fur, is beneficial to forming a physical barrier on pet wounds so as to reduce or even avoid wound bacterial infection, is beneficial to embodying non-toxicity and non-irritation on pet cells and skin and not generating allergy, is convenient to prevent bacteria from generating drug resistance, is good in compatibility with pets, and is good in reliably removing foreign matters on the surface of the pet skin so as to embody excellent deodorizing effects.
The invention also aims to provide a preparation method of the pet spray dressing, which has short process steps, does not need to depend on complex equipment and does not have harsh process elements, meets the requirement of industrial scale-up production, and can ensure that the technical effect of the pet spray dressing prepared by the method is comprehensively embodied.
In order to embody the primary task of the invention, the technical scheme provided by the invention is as follows: a pet spray dressing comprising per 1000g weight of finished spray dressing: 0.25-4g of octenidine hydrochloride, 0.06-4.8g of sodium alginate, 2.4-24g of 2-phenoxyethanol, 0.5-10g of polylysine hydrochloride, 0.5-5g of betaine, 0.5-20g of glycerol and the balance of pure water.
A pet spray dressing comprising per 1000g weight of finished spray dressing: octenidine hydrochloride 0.25g, sodium alginate 4.8g, 2-phenoxyethanol 13.2g, polylysine hydrochloride 10g, betaine 2.6g and glycerol 0.5g, the rest is pure water.
A pet spray dressing comprising per 1000g weight of finished spray dressing: 4g of octenidine hydrochloride, 0.06g of sodium alginate, 24g of 2-phenoxyethanol, 3g of polylysine hydrochloride, 5g of betaine, 10g of glycerol and the balance of pure water.
A pet spray dressing comprising per 1000g weight of finished spray dressing: octenidine hydrochloride 2.1g, sodium alginate 2.43g, 2-phenoxyethanol 2.4g, polylysine hydrochloride 0.5g, betaine 0.5g and glycerol 20g, the rest is pure water.
A pet spray dressing comprising per 1000g weight of finished spray dressing: 1.2g of octenidine hydrochloride, 0.9g of sodium alginate, 18g of 2-phenoxyethanol, 6g of polylysine hydrochloride, 3.5g of betaine, 6g of glycerol and the balance of pure water.
In order to embody another task of the present invention, the technical solution provided by the present invention is: a preparation method of the pet spray dressing comprises the following steps:
A) preparing a first solution, namely adding 0.5-5g of betaine into 200g of pure water 100 and stirring for dissolving, adding 0.25-4g of octenidine hydrochloride, and continuously stirring until the mixture is dissolved to obtain the first solution;
B) preparing a second solution, namely adding 0.5-10g of polylysine hydrochloride into 400g of pure water and stirring the mixture under a heating state until the polylysine hydrochloride is dissolved, adding 0.06-4.8g of sodium alginate, and continuously stirring the mixture until the sodium alginate is dissolved to obtain the second solution;
C) preparing a third solution, namely adding 0.5-20g of glycerol into 550g of pure water of 300-;
D) and (3) preparing a finished product, namely adding the first solution obtained in the step A), the second solution obtained in the step B) and the third solution obtained in the step C) into a volumetric flask of 1000ml, adding pure water to the volumetric flask to reach the volume of 1000ml, standing, filtering and filling to obtain the finished product of the pet spray dressing.
In a particular embodiment of the invention, the heating temperature in step B) is 40-60 ℃.
In another specific embodiment of the present invention, the time of said standing in step D) is 30-60 min.
In a further particular embodiment of the invention, the filtration in step D) is a filtration with a filter membrane having a pore size of 0.45. mu.m.
In still another specific embodiment of the present invention, the pet spray dressing in step D) forms a film having a thickness of 0.06-0.12mm and a particle size of 260-350nm after spraying.
According to one of the technical effects of the technical scheme provided by the invention, as sodium alginate is adopted in the formula, a physical barrier effect can be formed on a wound, and the occurrence of bacterial infection can be effectively reduced; secondly, because the composition has no cytotoxicity to pets, no irritation or allergy to pet skin, the composition can not be resisted by discomfort of pets; thirdly, because the octenidine hydrochloride with reasonable amount is used in the formula, the antibacterial composition has effective antibacterial activity on gram-positive bacteria, gram-negative bacteria and fungi, and the antibacterial composition can not cause the bacteria to generate drug resistance after being used for a long time, thereby having better biocompatibility with pets; fourthly, because the octenidine hydrochloride has strong residual effect on the skin surface, the long-acting antibacterial effect is excellent, and good antibacterial effect can be still observed after 24 hours of spraying; fifthly, as the 2-phenoxyethanol can enhance the sterilization effect of the octenidine hydrochloride, the sterilization effect of the pet spray dressing of the invention can be reflected to an extreme degree; sixthly, because the betaine selected in the formula is used as the surfactant, the skin can be cleaned, foreign matters on the surface of the skin of the pet can be removed, and an excellent deodorization effect is achieved; seventhly, the provided method has simple process steps and mild preparation conditions, can meet the requirement of industrial scale-up production, and can ensure that the prepared pet spray dressing can fully honor the technical effect.
Detailed Description
Example 1:
the preparation of a 1000g weight pet spray dressing comprises the following steps:
A) preparing a first solution, namely adding 2.6g of betaine into 120g of pure water (deionized water) (the same below), stirring for dissolving, stirring for 3min, adding 0.25g of octenidine hydrochloride, and continuously stirring until dissolving to obtain a first solution;
B) preparing a second solution, namely adding 10g of polylysine hydrochloride into 400g of pure water, stirring the mixture at the heating temperature of 40 ℃ until the polylysine hydrochloride is dissolved, stirring the mixture for 10min, adding 4.8g of sodium alginate, and continuously stirring the mixture until the sodium alginate is dissolved to obtain a second solution;
C) preparing a third solution, namely adding 0.5g of glycerol into 300g of pure water, stirring and dissolving, stirring for 3min, adding 13.2g of 2-phenoxyethanol, and continuously stirring until the glycerol is dissolved to obtain a third solution;
D) and (3) preparing a finished product, namely preparing the pet spray dressing, adding the first solution obtained in the step A), the second solution obtained in the step B) and the third solution obtained in the step C) into a volumetric flask of 1000ml, adding pure water to a constant volume of 1000ml, standing for 40min, filtering by using a filter membrane with the aperture of 0.45 mu m, and filling after filtering to obtain the pet spray dressing, wherein the thickness of a film formed by the sprayed pet spray dressing is 0.9mm, and the formed particle size is 250 nm.
Example 2:
the preparation of a 1000g weight pet spray dressing comprises the following steps:
A) preparing a first solution, namely adding 5g of betaine into 200g of pure water (deionized water) (the same below), stirring for dissolving, stirring for 6min, adding 4g of octenidine hydrochloride, and continuously stirring until dissolving to obtain a first solution;
B) preparing a second solution, namely adding 3g of polylysine hydrochloride into 350g of pure water, stirring at the heating temperature of 60 ℃ until the polylysine hydrochloride is dissolved, stirring for 8min, adding 0.06g of sodium alginate, and continuously stirring until the sodium alginate is dissolved to obtain a second solution;
C) preparing a third solution, namely adding 10g of glycerol into 470g of pure water, stirring and dissolving, stirring for 5min, adding 24g of 2-phenoxyethanol, and continuously stirring until the glycerol is dissolved to obtain a third solution;
D) and (3) preparing a finished product, namely preparing the pet spray dressing, adding the first solution obtained in the step A), the second solution obtained in the step B) and the third solution obtained in the step C) into a volumetric flask of 1000ml, adding pure water to a constant volume of 1000ml, standing for 60min, filtering by using a filter membrane with the aperture of 0.45 mu m, and filling after filtering to obtain the pet spray dressing, wherein the thickness of a film formed after spraying of the pet spray dressing is 0.12mm, and the formed particle size is 260 nm.
Example 3:
the preparation of a 1000g weight pet spray dressing comprises the following steps:
A) preparing a first solution, namely adding 0.5g of betaine into 100g of pure water (deionized water) (the same below), stirring for dissolving, stirring for 5min, adding 2.1g of octenidine hydrochloride, and continuously stirring until dissolving to obtain a first solution;
B) preparing a second solution, namely adding 0.5g of polylysine hydrochloride into 200g of pure water, stirring the mixture at the heating temperature of 45 ℃ until the polylysine hydrochloride is dissolved, stirring the mixture for 5min, adding 2.43g of sodium alginate, and continuously stirring the mixture until the sodium alginate is dissolved to obtain a second solution;
C) preparing a third solution, namely adding 20g of glycerol into 550g of pure water, stirring and dissolving, stirring for 4min, adding 2.4g of 2-phenoxyethanol, and continuously stirring until the glycerol is dissolved to obtain a third solution;
D) and (3) preparing a finished product, namely preparing the pet spray dressing, adding the first solution obtained in the step A), the second solution obtained in the step B) and the third solution obtained in the step C) into a volumetric flask of 1000ml, adding pure water to a constant volume of 1000ml, standing for 30min, filtering by using a filter membrane with the aperture of 0.45 mu m, and filling after filtering to obtain the pet spray dressing, wherein the thickness of a film formed after spraying of the pet spray dressing is 0.06mm, and the formed particle size is 320 nm.
Example 4:
the preparation of a 1000g weight pet spray dressing comprises the following steps:
A) preparing a first solution, namely adding 3.5g of betaine into 160g of pure water (deionized water) (the same below), stirring and dissolving, stirring for 4min until the betaine is dissolved, adding 1.2g of octenidine hydrochloride, and continuously stirring until the octenidine hydrochloride is dissolved to obtain a first solution;
B) preparing a second solution, namely adding 6g of polylysine hydrochloride into 300g of pure water, stirring at the heating temperature of 55 ℃ until the polylysine hydrochloride is dissolved, stirring for 6min, adding 0.9g of sodium alginate, and continuously stirring until the sodium alginate is dissolved to obtain a second solution;
C) preparing a third solution, namely adding 6g of glycerol into 400g of pure water, stirring and dissolving the glycerol, stirring the mixture for 6min, adding 18g of 2-phenoxyethanol, and continuously stirring the mixture until the glycerol is dissolved to obtain a third solution;
D) and (3) preparing a finished product, namely preparing the pet spray dressing, adding the first solution obtained in the step A), the second solution obtained in the step B) and the third solution obtained in the step C) into a volumetric flask of 1000ml, adding pure water to a constant volume of 1000ml, standing for 50min, filtering by using a filter membrane with the aperture of 0.45 mu m, and filling after filtering to obtain the pet spray dressing, wherein the thickness of a film formed after spraying of the pet spray dressing is 0.1mm, and the formed particle size is 300 nm.
The Zeta potential and the resulting particle size of the pet spray dressings obtained from examples 1 to 4 above are shown in table 1. The detection instrument is a Zetasizer Nano-ZS90 Markov particle size Analyzer, Marvin instruments Co., British.
Table 1 average particle size of pet spray dressing (n ═ 3)
| Examples | Example 1 | Example 2 | Example 3 | Example 4 |
| Particle size (nm) | 250 | 260 | 320 | 300 |
| Zeta(mV) | -0.34±0.07 | -0.41±0.08 | -0.43±0.07 | -0.38±0.08 |
In order to test the bacteriostatic effect of the pet spray dressing of the present invention, the applicant performed a bacteriostatic test on the pet spray dressings obtained in examples 1 to 4 of the present invention according to the fourth 1121 bacteriostatic efficacy test method of pharmacopoeia of the people's republic of china 2015:
test example 1:
the test procedure was as follows:
a) sterilizing a plate and a suction pipe for experiments in an electrothermal blowing dry box at 180 ℃ for 2 hours for later use;
b) preparing a fresh culture of trichophyton rubrum, and culturing the trichophyton rubrum in an incubator at the temperature of 30-35 ℃ for 18-24 hours.
c) Preparing the fresh culture in the step b) into bacterial suspension with the bacteria content of less than 100cfu (/ 50-100 cfu) per 1ml by adopting a liquid culture direct dilution method and using a sterile sodium chloride solution with the mass percent ratio concentration of 0.9%;
d) sucking 1ml of trichophyton rubrum suspension into a plate, and adding 1ml of the pet spray dressing obtained in any one of embodiments 1 to 4 of the invention;
e) adding 1ml of the pet spray dressing obtained in any one of the embodiments 1 to 4 of the invention into a blank plate;
f) respectively injecting sterilized tryptone soy agar into an experimental plate, and culturing for 48 hours in an incubator at the temperature of 30-35 ℃;
g) and observing the record, and completing the corresponding experimental record.
Test example 2:
a) sterilizing a plate and a suction pipe for experiments in an electrothermal blowing dry box at 180 ℃ for 2 hours for later use;
b) preparing a fresh culture of the microsporidia canis, and culturing the fresh culture in an incubator at the temperature of 30-35 ℃ for 18-24 hours.
c) Preparing the fresh culture in the step b) into bacterial suspension with the bacteria content of less than 100cfu (/ 50-100 cfu) per 1ml by adopting a liquid culture direct dilution method and using a sterile sodium chloride solution with the mass percent ratio concentration of 0.9%;
d) sucking 1ml of the canine microspore bacterial suspension into a plate, and adding 1ml of the pet spray dressing obtained in any one of the embodiments 1 to 4 of the invention;
e) adding 1ml of the pet spray dressing obtained in any one of examples 1 to 4 of the invention into a blank plate;
f) respectively injecting sterilized tryptone soy agar into an experimental plate, and culturing for 48 hours in an incubator at the temperature of 30-35 ℃;
g) and observing the record, and completing the corresponding experimental record.
Test example 3:
a) sterilizing a plate and a suction pipe for experiments in an electrothermal blowing dry box at 180 ℃ for 2 hours for later use;
b) preparing a fresh culture of malassezia furfur, and culturing the malassezia furfur in an incubator at the temperature of 30-35 ℃ for 18-24 hours.
c) Preparing the fresh culture in the step b) into bacterial suspension with the bacteria content of less than 100cfu (/ 50-100 cfu) per 1ml by adopting a liquid culture direct dilution method and using a sterile sodium chloride solution with the mass percent ratio concentration of 0.9%;
d) sucking 1ml of malassezia furfur suspension into a plate, and adding 1ml of the pet spray dressing obtained in any one of embodiments 1 to 4 of the invention;
e) adding 1ml of the pet spray dressing obtained in any one of examples 1 to 4 of the invention into a blank plate;
f) respectively injecting sterilized tryptone soy agar into an experimental plate, and culturing for 48 hours in an incubator at the temperature of 30-35 ℃;
g) and observing the record, and completing the corresponding experimental record.
The results obtained in the above test examples 1, 2 and 3 are shown in the following table:
as can be seen from the table, the pet spray dressing provided by the invention can achieve the effect of completely killing trichophyton rubrum, microsporum canis and malassezia furfur.
Claims (10)
1. A pet spray dressing comprising per 1000g weight of finished spray dressing: 0.25-4g of octenidine hydrochloride, 0.06-4.8g of sodium alginate, 2.4-24g of 2-phenoxyethanol, 0.5-10g of polylysine hydrochloride, 0.5-5g of betaine, 0.5-20g of glycerol and the balance of pure water.
2. The pet spray dressing of claim 1, comprising per 1000g weight of finished spray dressing: octenidine hydrochloride 0.25g, sodium alginate 4.8g, 2-phenoxyethanol 13.2g, polylysine hydrochloride 10g, betaine 2.6g and glycerol 0.5g, the rest is pure water.
3. The pet spray dressing of claim 1, comprising per 1000g weight of finished spray dressing: 4g of octenidine hydrochloride, 0.06g of sodium alginate, 24g of 2-phenoxyethanol, 3g of polylysine hydrochloride, 5g of betaine, 10g of glycerol and the balance of pure water.
4. The pet spray dressing of claim 1, comprising per 1000g weight of finished spray dressing: octenidine hydrochloride 2.1g, sodium alginate 2.43g, 2-phenoxyethanol 2.4g, polylysine hydrochloride 0.5g, betaine 0.5g and glycerol 20g, the rest is pure water.
5. The pet spray dressing of claim 1, comprising per 1000g weight of finished spray dressing: 1.2g of octenidine hydrochloride, 0.9g of sodium alginate, 18g of 2-phenoxyethanol, 6g of polylysine hydrochloride, 3.5g of betaine, 6g of glycerol and the balance of pure water.
6. A method of making the pet spray dressing of claim 1, comprising the steps of:
A) preparing a first solution, namely adding 0.5-5g of betaine into 200g of pure water 100 and stirring for dissolving, adding 0.25-4g of octenidine hydrochloride, and continuously stirring until the mixture is dissolved to obtain the first solution;
B) preparing a second solution, namely adding 0.5-10g of polylysine hydrochloride into 400g of pure water and stirring the mixture under a heating state until the polylysine hydrochloride is dissolved, adding 0.06-4.8g of sodium alginate, and continuously stirring the mixture until the sodium alginate is dissolved to obtain the second solution;
C) preparing a third solution, namely adding 0.5-20g of glycerol into 550g of pure water of 300-;
D) and (3) preparing a finished product, namely adding the first solution obtained in the step A), the second solution obtained in the step B) and the third solution obtained in the step C) into a volumetric flask of 1000ml, adding pure water to the volumetric flask to reach the volume of 1000ml, standing, filtering and filling to obtain the finished product of the pet spray dressing.
7. The method of making a pet spray dressing as claimed in claim 6, wherein the temperature of the heating in step B) is 40-60 ℃.
8. The method of preparing a pet spray dressing as claimed in claim 6, wherein the time of the standing in step D) is 30-60 min.
9. The method of claim 6, wherein the filtering in step D) is performed with a filter membrane having a pore size of 0.45 μm.
10. The method for preparing pet spray dressing according to claim 6, wherein the thickness of the film formed after spraying the pet spray dressing in the step D) is 0.06-0.12mm and the formed particle size is 260-350 nm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114832150A (en) * | 2022-04-15 | 2022-08-02 | 北京天珠药业有限公司 | Spray dressing for promoting barrier repair of antibacterial convergent wound |
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