KR20130123489A - Microbicide compositions using vitamin b1 derivatives and effective microorganism - Google Patents

Abstract

The present invention comprises: vitamin B1 lauryl sulfate which is a vitamin B1 derivative capable of controlling effective microorganisms without killing the effective microorganisms while having a sterilization effect, an algicidal effect, an antibacterial effect and an antimicrobial effect; organic acid, wherein at least one among citric acid, lactic acid, malic acid, adipic acid, phytic acid and glycine is mixed; an effective microorganism culture medium, wherein effective microorganisms having sterilizing power, antibacterial activity and antioxidative activity are dissolved; and purified water, thereby increasing sterilizing power, antibacterial activity, antioxidativity rate and the duration thereof. And, the purpose of the present invention is to provide a microbicide composition using the effective microorganisms and vitamin B1 derivative, which is environment-friendly and harmless to humans because of the nontoxic properties.

Description

Microbicide compositions using vitamin B1 derivatives and Effective Microorganism

The present invention relates to a bactericidal composition using a vitamin-B1 derivative and a useful microorganism, and in detail, vitamin-B1 lauryl sulfate, a citric acid, lactic acid, It is composed of an organic acid mixed with at least one of malic acid, adipic acid, phytic acid and glycine, a useful microorganism culture medium in which useful microorganisms having antimicrobial, deodorizing and antioxidant functions are dissolved, and purified water. The present invention relates to a sterilizing composition using vitamin-B1 derivatives and useful microorganisms that are not only toxic but have no toxicity and are environmentally friendly and harmless to humans.

Fungicides generally refer to drugs that inhibit or eliminate the growth of microorganisms and bacteria, and are used in various fields such as humidifiers, foods, medical devices, green algae removal and swimming pools. In particular, with increasing interest in hygiene and health, the field of use of fungicides is gradually expanding, and various studies are being conducted to develop fungicides that are harmless to the human body.

In addition, the user is used for sterilization after mixing the sterilizer with purified water and a specific solution at a specific concentration necessary for sterilization because the sterilizer is very strong and antibacterial. For example, Lax (chlorine disinfectant) used to eradicate toilet strains is used after diluting Lax in water to a specific concentration.

As used, conventionally used fungicides (hereinafter, referred to as conventional fungicides) have strong bactericidal and algicidal properties, such as chlormethylisothiazoline / methylisothiazoline (CMIT / MIT) for humidifier cleaners and slime cleaners. : Chloromethyliso- thiazolinone / Methylisothiazolinone, disinfectant known as general purpose disinfectant and disinfectant (Lax, high bleach powder, chlorine dioxide aqueous solution, isocyanuric formulation), PHMG (Polyhexamethylene guanidine) and PGH (Oligo) recently used as raw materials for humidifier cleaner (2- (2-ethoxy) ethoxyethyl guanidine chloride)) is used.

However, PHMG (polyhexamethylene guanidine) and PGH (Oligo (2- (2-ethoxy) ethoxyethyl guanidine chloride)) have been used mainly in humidifier cleaners, wet tissues and nonwoven fabrics because they have no smell and have excellent antiseptic and antibacterial effects. When accumulated in the human lungs by the inhalation of humans, the toxic component is greatly increased over time, causing problems that damage the lungs.

In addition, CMIT / MIT (Chloromethyl-isothiazolinone / Methylisothiazolinone) is easy to manufacture and has excellent sterilization and algae function, but it stimulates the skin mucous membrane of the human body, stimulates the skin mucous membrane of allergy, and causes allergic reaction. Has

In addition, quaternary ammonium (Ammounium), glutaraldehyde, copper sulfate and chlorine disinfectants are used for air conditioning or general slime control agents because of their excellent sterilization and antibacterial activity, but quaternary ammonium is the CMIT / Like MIT, it has toxic properties that are harmful to the human body, and glutaraldehyde also has toxic properties that irritate the eyes, nose, throat, and skin when in contact with the human body. This causes a problem that harms the human body.

In addition, chlorine disinfectants used as general purpose disinfectants, in particular sodium hypochlorite (lac), calcium hypochlorite (calcium hypochlorite), trichloroisocyanuric acid (TCCA), isocyanuric dichloride Sodium Dichloroisocyanurate is used in various fields because of its excellent cost-effective sterilization and antibacterial properties, but it also causes trihalomethane, in which halogen-based chemicals such as chlorine (Cl) act as carcinogens. In case of contact with human body, chlorine is mixed with human skin waste to produce carcinogenic substance called chloramine, and if it comes into contact with device, it has high oxidation rate and corrode the device. There are disadvantages such as giving.

In addition, all of the above drugs are harmful to the human body, so when used in the algae harm to aquatic life.

As described above, in order to improve the problems of the compositions applied to the conventional fungicides, various researches have been conducted on the fungicides applying vitamin-B1 derivatives having excellent sterilization and algicidal properties while being harmless to the human body.

The food preservative composition (hereinafter referred to as a conventional composition) disclosed in Korean Patent Publication No. 10-2005-0105876 (name of the invention: a composition for sterilization and preservation of food) is vitamin B1 lauryl sulfate, lactic acid, phytic acid And at least one organic acid selected from the group consisting of adipic acid, and glycine.

In addition, the conventional composition has the advantage that the vitamin B1 lauryl sulfate is harmless to the human body, has a bactericidal effect, does not cause a change in taste or aroma of food, and has an excellent bactericidal effect, which will be described later. It does not sterilize the group.

However, when the conventional composition is actually used in the field, a problem occurs that the sterilization power and antimicrobial power fall.

In addition, the conventional composition has a problem that sterilization, algae and antimicrobial effect is sharply dropped after a certain time does not last long.

In addition, the conventional composition has a problem in that it does not properly perform the role of the disinfectant due to low sterilization power when applied to various fields because the sterilization power is lower than the antimicrobial activity.

The present invention is to solve this problem, it is to provide a sterilizing composition that greatly increases the sterilization and algae capacity than the vitamin B1 lauryl sulfate as a vitamin-B1 derivative is used alone.

In addition, another solution of the present invention is to provide a sterilizing composition that is non-toxic and harmless to the human body while having high sterilizing and antibacterial properties.

In addition, another solution of the present invention is to provide an antimicrobial composition having a long duration of such effects as well as increasing the antioxidant power by adding an appropriate amount of useful microorganisms.

In addition, another solution of the present invention is to provide a sterilizing composition which increases the solubility of vitamin B1 lauryl sulfate by increasing the water solubility.

Solution of the present invention for solving the above problems is 5 to 20% by weight of vitamin B1 lauryl sulfate; 0-10 wt% of useful microorganisms; 5-50% by weight of organic acid; It includes 50 to 90% by weight of purified water.

In addition, the useful microorganism group in the present invention is preferably formed by mixing 80 to 90% by weight of rice water, 5 to 10% by weight molasses, and 5 to 10% by weight of the useful microorganism active liquid.

In the present invention, the useful microbial group is preferably cultured for at least 7 days after mixing.

In the present invention, the organic acid is preferably a mixture of at least one or more of citric acid, lactic acid, malic acid, adipic acid, phytic acid and glycine.

In addition, in the present invention, it is preferable to use the concentration of 0.5 to 20% by weight to be used for cleaning and disinfection.

According to the present invention having the above problems and solutions, the bactericidal power, the killing power and the antimicrobial power are increased than when vitamin B1 lauryl sulfate is used alone.

In addition, according to the present invention, vitamin B1 lauryl sulfate has a strong bactericidal and antioxidant effect when used in combination with useful microorganisms due to its unique properties of sterilizing and disinfecting various bacteria, etc. with its unique bactericidal power. do.

In addition, according to the present invention there is no toxicity, harmless to the human body, and can be used as a fungicide in various fields because it is environmentally friendly.

In addition, according to the present invention, not only the antioxidant rate is increased by the useful microorganism, but also the antioxidative and bactericidal effects last for a long time, thereby increasing the sustained effect relative to the amount used.

In addition, according to the present invention, the organic acid is added, so that the water solubility of the vitamin B1 lauryl sulfate increases, so that the bactericidal and algicidal ability is increased.

Figure 1 is a real picture showing the degree of growth by concentration of Example 1 for the black mold.

Hereinafter, an embodiment of the present invention will be described with reference to the accompanying drawings.

The sterilizing composition according to one embodiment of the present invention is a microbial group culture medium formed by mixing vitamin B1 lauryl sulfate, a vitamin-B1 derivative having a sterilization, algae, antibacterial and antimicrobial function, and rice water, molasses and useful microbial active liquids. , An organic acid formed by mixing at least one of citric acid, lactic acid, malic acid, adipic acid, phytic acid and glycine, and water. At this time, the sterilizing composition is composed of 5 to 20% by weight of vitamin B1 lauryl sulfate, 0 to 10% by weight of the useful microorganism culture medium, 5 to 50% by weight of organic acid, and 50 to 90% by weight of purified water.

Vitamin B1 lauryl sulfate is a harmless vitamin-B1 derivative having strong antibacterial and harmless properties to the human body, and not only increases the antibacterial activity of the bactericidal composition of the present invention but also causes bacteria, microorganisms, fungi, yeast, E. coli and algae ( Algae) etc. are removed efficiently.

In addition, vitamin B1 lauryl sulfate is represented by the following formula (1).

Table 1 shows the water solubility of vitamin B1 lauryl sulfate.

Temperature (℃) Vitamin B1 Lauryl sulfate  Water solubility (%) 0 0.01 10 0.01 25 0.21 30 24 40 64 50 100

Lauryl sulfate is generally prepared in powder form due to easy precipitation and low stability. However, vitamin B1 lauryl sulfate is very poorly soluble in water as shown in Table 1, and is generally insoluble in water. . Accordingly, a method of adding a high concentration of acetic acid or a high concentration of ethyl alcohol to prepare a vitamin B1 lauryl sulfate as a liquid formulation is known and used, but since acetic acid and ethyl alcohol cause acetic acid and alcohol odor, Esau adds organic acids to make vitamin B1 lauryl sulfate dissolvable in water.

In addition, vitamin B1 lauryl sulfate is mixed 5 to 20% by weight in the sterilizing composition of one embodiment of the present invention. At this time, if the content of vitamin B1 lauryl sulfate is less than 5% by weight, the bactericidal effect of vitamin B1 lauryl sulfate itself is lowered, and the sterilizing power of the composition for sterilization is lowered. By adding a separate alcoholic or acetic acid solvent to increase the water solubility, there is a problem that the sterilization and antibacterial properties are poor.

Useful microorganism culture medium is composed of 5 to 10% by weight of useful microorganisms, such as yeast, lactic acid bacteria, actinomycetes and photosynthetic bacteria, 5 to 10% by weight of molasses, 5 to 10% by molasses, and 80 to 90% by weight of rice water. . At this time, the useful microorganism group culture medium configured as described above is formed by culturing for 7 days to 8 days after the above-mentioned components are mixed, and in the present invention, 5 to 10% by weight of the useful microorganism active solution of Ever Miracle Co., Ltd. was applied. .

The useful microorganisms of such a useful microorganism group culture medium have a characteristic of co-existing with each other to generate a sulfated substance after a certain time, and the generated sulfated substance has a property of inhibiting oxidation and decay of the substance, so that the antimicrobial activity of the sterilizing composition Will increase.

Organic acids have a strong freshness-retaining action, increase the water solubility of vitamin B1 lauryl sulfate, have their own antibacterial and bacteriostatic properties, and increase the bactericidal and antimicrobial properties when mixed with vitamin B1 lauryl sulfate.

In addition, the organic acid has a weak acidic force, and has a relatively high molecular weight, so that at least one or more of citric acid, lactic acid, malic acid, adipic acid, phytic acid, and glycine are mixed, and the content is 5 to 50% by weight. At this time, if the organic acid is less than 5% by weight, the property of the organic acid itself is not only lowered, but the water solubility of vitamin B1 lauryl sulfate does not increase significantly, and the bactericidal and antimicrobial powers are not greatly increased. There is a fear that the organic salt is precipitated due to the above.

Organic acids also increase the water solubility of vitamin B1 lauryl sulfate, and increase the bactericidal and antimicrobial activity of vitamin B1 lauryl sulfate when used alone. At this time, since the technique of increasing the bactericidal and antimicrobial properties by mixing the organic acid and vitamin B1 lauryl sulfate is described in Japanese Patent Application Laid-Open No. 11-2063565 and Korean Patent Publication No. 10-2005-0105876, detailed description thereof will be omitted. .

Hereinafter, an example for the sterilizing composition which is an embodiment of the present invention will be described in more detail. The following embodiments are for illustrative purposes only and do not limit the scope of protection of the present invention.

20% by weight of vitamin B1 lauryl sulfate;

90% by weight of rice water, 5% by weight of the useful microorganism active liquid, and 10% by weight of the useful microorganism culture medium formed of 5% by weight molasses;

50% by weight citric acid and 10% by weight organic acid formed by mixing 50% by weight lactic acid;

Sterilizing composition comprising 50% by weight of purified water.

Comparative Example 1

20% by weight of vitamin B1 lauryl sulfate;

50% by weight citric acid and 10% by weight organic acid formed by mixing 50% by weight lactic acid;

Sterilizing composition comprising 70% by weight of purified water.

Table 2 shows the containing components of Example 1 and Comparative Example 1.

Configuration Comparative Example 1 Example 1
ship

synthesis Vitamin B1 Lauryl Sulfate 20% 20% Organic acid Lactic acid 5% 5% Citric acid 5% 5% Useful Microbial Cultures × 20% Purified water 70% 50%

* The content of the composition is weight percent

The useful microorganism culture medium of Table 2 is formed by mixing 5% by weight of the useful microorganism active solution and 5% by weight molasses to 90% by weight of rice water as described above.

-Bacterial production-

Representative strains Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and Black fungus (Aspergillus niger) to test the antimicrobial activity of Example 1 and Comparative Example 1 After the pre-cultivation of the test bacteria consisting of (Pre culture) was inoculated into the sample of Example 1 and Comparative Example 1, and cultured shaking at 37 ℃ for 24 hours.

Table 3 shows the degree of growth by concentration of Example 1 for E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and black molds.

Concentration (ppm)

Germ

Yeast
mold E.Coli S.Aureus P.aeruginosa C.albicans A. niger 0 +++ +++ +++ +++ +++ 10 - - - + + 20 - - - - - 50 - - - - - 100 - - - - -

Visual discrimination criteria +: Microorganism microbial breeding, ++: Microorganism microbial breeding, +++: Microorganism microbial breeding,-: Not detected)

Looking at Table 3, when Example 1, which is an embodiment of the present invention, is used in the strain at a concentration of 10 ppm, bactericidal and antimicrobial activities against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa are very high, and visually discriminating that E. coli, Staphylococcus aureus and Pseudomonas aeruginosa are all removed. In addition, the bactericidal and antimicrobial activity against yeast and mold are also high, and it can be seen that most of these fungi are removed.

In addition, it can be seen that Example 1 can be removed from both yeast and mold when used in a strain of 20 ppm or more.

Figure 1 is a real picture showing the degree of growth by concentration of Example 1 for the black mold.

As shown in Fig. 1 (a) as shown in Figure 1 (a) Example 1 is mixed with purified water 99.983% by weight of 0.017% by weight when looking at the growth of the black mold can be seen that the black mold 10 is a lot of breeding In addition, as shown in (b) of FIG. 1, when Example 1 is mixed with 0.035% by weight of 99.965% by weight of purified water, it can be seen that the black mold 10 has a reduced breeding degree compared to (a) of FIG. 1, As shown in (c) of FIG. 1, when Example 1 is used in an amount of 0.070 wt% and mixed with 99.930 wt% of purified water, the growth of black mold (10) is significantly reduced compared to (a) and (b) of FIG. It can be seen that 10) is mostly removed.

Table 4 shows the degree of proliferation by concentration of Comparative Example 1 for Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans and black molds.

Concentration (ppm)

Germ

Yeast
mold E.Coli S.Aureus P.aeruginosa C.albicans A. niger 0 +++ +++ +++ +++ +++ 10 + + + + + 20 + - - + + 50 - - - - - 100 - - - - -

(Based on visual discrimination +: microorganism microbial breeding, ++: microorganism microbial breeding, +++: microorganism microbial breeding,-: not detected)

Looking at Table 4, Comparative Example 1, which is not added to the useful microorganisms, such as the sterilizing composition of the present invention can be seen that the concentration of E. coli, Candida albicans and black fungus is partially reduced when the concentration is 10ppm, Staphylococcus and It can be seen that the proliferation of Pseudomonas aeruginosa is greatly reduced.

In addition, in Comparative Example 1, when the concentration is 20 ppm, Escherichia coli, Candida albicans and black mold are greatly reduced in breeding degree, and staphylococcus and Pseudomonas aeruginosa are not detected.

In addition, when comparing Comparative Example 1 to Example 1, it can be seen that Example 1 of the present invention has excellent bactericidal and antimicrobial activities against all strains.

Example 1 Compositions Usable as Humidifier Cleaner

Use Example 1 is formed of 20% by weight of vitamin B1 lauryl sulfate, 10% by weight of an organic acid, 20% by weight of a useful microorganism culture medium, and 50% by weight of purified water, and the composition of the humidifier cleaner as in Example 1. It can be used in place of CMIT / MIT, PHMG, PGH, Sodium dichloro Isocyanurate (SDIC).

In addition, the sterilizing composition of Example 1 is added 0.5 to 20.0% by weight when mixed in purified water.

Use Example 2: Composition that can be used as a baby bottle disinfectant

Use Example 2 is formed of 20% by weight of vitamin B1 lauryl sulfate, 10% by weight of organic acid, 20% by weight of useful microorganism culture medium, and 50% by weight of purified water as in Example 2. At this time, the sterilizing composition of Example 2 is added 2.0 to 25.0% by weight when mixed in purified water.

In addition, SDIC is mainly used as a baby bottle disinfectant composition, but SDIC has a low chlorine odor, so it is difficult to use as a baby bottle disinfectant due to the toxicity of chlorine itself.

However, Example 2, which is an embodiment of the present invention, does not include chlorine, and thus does not have harmful toxicity to infants. Therefore, it can be used as a baby bottle disinfectant instead of the existing SDIC.

-Example 3 composition usable as a fungicide for food and beverage

Use Example 3 is formed of 20% by weight of vitamin B1 lauryl sulfate, 10% by weight of organic acid, 20% by weight of useful microorganism culture medium, and 50% by weight of purified water as in Example 3. At this time, the sterilizing composition of Example 3 is added 5.0 to 30.0% by weight when mixed in purified water.

In addition, SDIC is mainly used as a baby bottle disinfectant composition, but SDIC has a low chlorine odor, so it is difficult to use as a baby bottle disinfectant due to the toxicity of chlorine itself.

However, Example 3 does not contain chlorine, so it is not toxic, so it can be used as a feeding bottle disinfectant for breast milk by replacing the existing SDIC, and the existing SDIC can overcome the problem of harming infants including toxicity.

Example 4 Compositions usable as Medical Sterilizers

Use Example 4 is formed of 20% by weight of vitamin B1 lauryl sulfate, 10% by weight of organic acid, 20% by weight of useful microorganism culture medium, and 50% by weight of purified water as in Example 4. Sterilization of Use Example 4 The composition for the addition is 5.0 to 30.0% by weight when mixed in purified water.

In addition, in the case of medical disinfectants, disinfectants similar to food and beverage disinfectants have been used and are manufactured and sold in the form of effervescent tablets. Therefore, the powder itself of Example 1 can be formulated into foamed tablets, and can be used to replace the conventional fungicide at a concentration of 30 to 70% by weight.

Example 5 Compositions usable as Swimming Pool Water Disinfectants

Use Example 5 is formed of 20% by weight of vitamin B1 lauryl sulfate, 10% by weight of organic acid, 20% by weight of useful microorganism culture medium, and 50% by weight of purified water as in Example 5. At this time, the sterilizing composition of Example 5 is added 0.5 to 5.0% by weight when mixed in purified water.

In general, the disinfectant for swimming pool water is almost conventionally used as a chlorine disinfectant, and in particular, it has an advantage of excellent cohesion and sterilization ability, but it is not only strong in chlorine odor but also expensive foreign products, so it is difficult to purchase sodium hypochlorite (lax) and highly bleached powder ( Disinfectants such as 60-70% calcium hypochlorite) are used.

Use Example 5 can be used in place of such a conventional disinfectant, and the addition of a coagulant for food additives such as PAC to Use Example 5 increases cohesion and bactericidal power, thereby preventing the occurrence of chloramine, a carcinogen.

Use Example 6: Composition that can be used as an air conditioning sterilizer for cooling towers

Use Example 6 is formed of 20% by weight of vitamin B1 lauryl sulfate, 10% by weight of organic acid, 20% by weight of useful microorganism culture medium, and 50% by weight of purified water as in Example 6. At this time, the sterilizing composition of Example 6 is added 0.5 to 50.0% by weight when mixed in purified water.

Generally, air conditioning sterilizers for sterilizing cooling towers include CMIT / MIT, and products such as quaternary ammonium, bromine, glutaraldehyde, etc., which are highly toxic to skin. However, these products are highly toxic and are not suitable for use as a disinfectant. Have

Use Example 7 Compositions Usable as Generalized Slime Control Agents

Use Example 7 is formed of 20% by weight of vitamin B1 lauryl sulfate, 10% by weight of organic acid, 20% by weight of useful microorganism culture medium, and 50% by weight of purified water as in Example 7. At this time, the sterilizing composition of Example 7 is added to 0.01 ~ 10.00% by weight when mixed in purified water.

In general, Slime is a strain that occurs frequently in places such as food factories, paper mills, and other industrial processes, and is conventionally used to remove such slimes by using CMIT / MIT compositions or copper sulfate-based compositions. However, since such a composition has certain toxic properties, use example 7 may be used in place of an existing product.

-Example 8: A composition that can be used as an algae remover in ponds where animals and plants live.

Use Example 8 is formed of 20% by weight of vitamin B1 lauryl sulfate, 10% by weight of organic acid, 20% by weight of useful microorganism culture medium, and 50% by weight of purified water as in Example 8. At this time, the sterilizing composition of Example 8 is added 0.01 to 0.50% by weight when mixed in purified water.

In general, as a conventional green algae remover, a copper sulfate-based composition is used, and copper sulfate has a weak toxic component but does not completely remove the toxic component, thereby causing a problem of aquatic life.

However, use example 8 of the present invention is not toxic and harmless to aquatic organisms because it is not toxic to humans, so it can be used in place of the conventional green algae remover.

Example 9 Compositions Usable as Preservatives of Wipes

Use Example 9 is formed of 20% by weight of vitamin B1 lauryl sulfate, 10% by weight of organic acid, 20% by weight of useful microorganism culture medium, and 50% by weight of purified water as in Example 9. At this time, the sterilizing composition of Example 9 is added to 0.05 ~ 1.00% by weight when mixed in purified water.

In general, wet tissues are exposed to fungi and bacteria during long-term storage, so the antiseptic and antiseptic ingredients of CMIT / MIT series contain about 0.05% of the antiseptic effect. However, as mentioned above, CMIT / MIT series compositions are toxic. This has a strong drawback.

In order to solve such a problem, use example 9 may be used in place of a conventional wet water preservative.

Claims (5)

5-20% by weight of vitamin B1 lauryl sulfate;
0-10 wt% of useful microorganisms;
5-50% by weight of organic acid;
Sterilizing composition comprising 50 to 90% by weight of purified water. The method of claim 1, wherein the useful microbial group is
Sterilizing composition, characterized in that formed by mixing 80 to 90% by weight of rice water, 5 to 10% by weight molasses, and 5 to 10% by weight of the useful microorganism active liquid. The sterilizing composition of claim 2, wherein the useful microorganism group is cultured for at least 7 days after mixing. The sterilizing composition of claim 1, wherein the organic acid is at least one of citric acid, lactic acid, malic acid, adipic acid, phytic acid, and glycine. The sterilizing composition according to any one of claims 1 to 4, which is used for cleaning and sterilizing using a concentration of 0.5 to 20% by weight.

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