CN110810424A - Bactericidal composition - Google Patents

Bactericidal composition Download PDF

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Publication number
CN110810424A
CN110810424A CN201810908260.7A CN201810908260A CN110810424A CN 110810424 A CN110810424 A CN 110810424A CN 201810908260 A CN201810908260 A CN 201810908260A CN 110810424 A CN110810424 A CN 110810424A
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benziothiazolinone
difenoconazole
composition
plants
plant
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CN201810908260.7A
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Inventor
罗昌炎
詹姆斯.T.布里斯托
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Jiangsu Rotam Chemical Co Ltd
Rotam Agrochem International Co Ltd
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Rotam Agrochem International Co Ltd
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Priority to CN202111655557.5A priority Critical patent/CN114365744A/en
Priority to CN201810908260.7A priority patent/CN110810424A/en
Publication of CN110810424A publication Critical patent/CN110810424A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00—Preservation or chemical ripening of fruit or vegetables
    • A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154—Organic compounds; Microorganisms; Enzymes

Abstract

The present invention relates to a fungicidal composition, in particular to a fungicidal composition for protecting plants or seeds from attack by pathogenic fungi. The invention provides a sterilization composition which contains active ingredients of benziothiazolinone and difenoconazole, wherein the weight ratio of the benziothiazolinone to the difenoconazole is 1:1-1: 50. The invention also relates to the use of said fungicidal compositions for controlling fungi and bacteria on cereals, legumes, fruit and fruit trees, vegetables, root vegetables, forage grasses, turf grass or turf, spice crops, flowering plants.

Description

Bactericidal composition
Technical Field
The present invention relates to a fungicidal composition, in particular to a fungicidal composition for protecting plants or seeds from attack by pathogenic fungi.
Background
At present, for disease control which is easy to generate resistance in agriculture, the pesticide varieties with different action mechanisms are optimally mixed, and if the mixture ratio is reasonable, the obvious synergistic effect can be generated, so that the field control effect is obviously better than that of each single agent. The bactericide containing a single active ingredient has certain defects in agricultural disease control, not only is pathogenic bacteria easy to generate drug resistance, but also is easy to cause pollution to food and environment after being continuously used for multiple times, and the defects can be overcome by reasonably mixing the active ingredients of the bactericide. The reasonable compounding ensures that the effective components generate synergistic action, can improve the control effect, reduce the using amount of the effective components, save the cost, delay the generation of drug resistance of pathogenic bacteria, and further can lighten or even avoid the pollution of pesticides to food and environment.
Difenoconazole (Difenodiconazole), chemical name: 2- [ 2-chloro-4- (4-chlorophenoxy) phenyl ] -1, 3-bis (1H-1, 2, 4-triazol-1-yl) -2-propanol. The molecular structural formula is as follows:
Figure DEST_PATH_IMAGE002
difenoconazole is known from CN 100451007C.
With regard to the activity of pesticides, in particular with regard to crop protection, one of the core problems of the research carried out in this technical field is the improvement of the properties, in particular in terms of biological activity, and the maintenance of this activity over a certain period of time.
Due to the ever-increasing environmental and economic requirements imposed on fungicides, for example on the spectrum of activity, toxicity, selectivity, application rates, residues and favourable feasibility of preparation, there may be problems, for example, with regard to resistance. Therefore, it is a continuing task to develop new fungicides that are superior in some respects to existing fungicides.
Disclosure of Invention
Aiming at the defects, the invention provides a sterilization composition which contains the active ingredients of benziothiazolinone and difenoconazole.
It has now surprisingly been found that the simultaneous, i.e. joint or separate, application of benziothiazolinone and difenoconazole, or the sequential application of benziothiazolinone and difenoconazole, leads to better control of phytopathogenic fungi than the individual compounds applied individually.
Therefore, the invention provides the bactericidal composition, the composition is obtained by binary compounding of the benziothiazolinone and the difenoconazole, so that the obtained composition has a gain effect on the prevention and treatment effect, the bactericidal spectrum is expanded, the effect of one medicine for multiple purposes is achieved, and the generation of drug resistance of germs is effectively slowed down or avoided. Surprisingly, the fungicidal activity of the fungicidal compositions according to the invention is significantly higher than the sum of the activities of the individual active compounds. In other words, there is an unpredictable, truly present synergistic effect, not just a supplementation of activity.
The synergistic effect is particularly pronounced when the active compounds are present in the fungicidal compositions of the present invention in a particular weight ratio. However, the weight ratio of the active compounds in the fungicidal compositions according to the invention can vary within certain limits.
The invention discloses a sterilization composition, which is realized by adopting the following technical scheme:
a germicidal composition, characterized by: contains the active ingredients of benziothiazolinone and difenoconazole, wherein the weight ratio of benziothiazolinone to difenoconazole is 1:1-1:50, preferably 1:5-1:40, preferably 1:5-1:35, more preferably 1:5-1:30, more preferably 1:5-1:25, more preferably 1:5-1:20, more preferably 1:8-1: 20.
The weight ratio of benziothiazolinone to phenylate diazole in the present invention may also be, for example, 1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,1:15,1:16,1:17,1:18,1:19,1:20,1:21,1:22,1:23,1:24,1:25,1:26,1:27,1:28,1:29,1:30,1:31,1:32,1:33,1:34,1:35,1:36,1:37,1:38,1:39,1:40,1:41,1:42,1:43,1:44,1:45,1:46,1:47,1:48,1:49,1: 50.
The sterilization composition is characterized in that: the sum of the mass of the benziothiazolinone and the mass of the difenoconazole accounts for 5% -90%, more preferably 5% -80%, more preferably 10% -75%, more preferably 10% -70%, more preferably 10% -65%, more preferably 10% -60%, more preferably 10% -55%, more preferably 10% -50% of the mass of the sterilization composition.
In the bactericidal composition of the present invention, the content of the benziothiazolinone and the difenoconazole may also be, for example: 1%,2%,3%,4%,5%,6%,7%,8%,9%,10%,11%,12%,13%,14%,15%,16%,17%,18%,19%,20%,21%,22%,23%,24%,25%,26%,27%,28%,29%,30%,31%,32%,33%,34%,35%,36%,37%,38%,39%,40%,41%,42%,43%,44%,45%,46%,47%,48%,49%,50%,51%,52%,53%,54%,55%,56%,57%,58%,59%,60%,61%,62%,63%,64%,65%,66%,67%,68%,69%,70%,71%,72%,73%,74%,75%,76%,77%,78%,79%,80%,81%,82%,83%,84%,85%,86%,87%,88%,89% and 90%.
A method for controlling phytopathogenic fungi comprises applying a fungicidal composition to the pathogenic fungi and/or to the environment thereof, or to the plant, parts of the plant, seeds, the soil, the area, the material or the space.
A method for controlling plant pathogenic bacteria comprises simultaneously applying benziothiazolinone and difenoconazole, or applying benziothiazolinone and difenoconazole respectively, or applying benziothiazolinone and difenoconazole sequentially.
A bactericidal composition comprises benziothiazolinone, difenoconazole, a filler and/or a surfactant.
A bactericidal composition can be prepared into any agriculturally allowable dosage form. The bactericidal composition is in the dosage form of aqueous suspension, seed treatment suspension, suspoemulsion, wettable powder, water dispersible granules, microcapsule suspension, granules, missible oil, microemulsion, emulsion in water, effervescent tablets, ultra-low volume liquid, hot fogging concentrate, dry suspension and oil suspension.
The bactericidal composition is used for preventing and treating cereals, legumes, fruit trees and fruits, vegetables, root vegetables, forage grasses, lawn grasses or turf, aromatic crops, flowers and the like.
The use of the fungicidal compositions for protecting plants, plant propagation material and plant organs that grow subsequently against fungal and bacterial attack. The plant propagation material is a seedling, rhizome, nursery seedling, cutting or seed.
Use of the fungicidal composition for the treatment of seeds to protect the seeds from fungal and bacterial attack.
The use of the fungicidal composition for treating the soil in which plants are growing to protect the plants from fungal and bacterial attack in the soil.
The use of the fungicidal compositions for controlling phytopathogenic or saprophytic fungi and bacteria, when applied to the locus where control is desired.
The bactericidal composition is used for preventing and treating diseases of fruits and vegetables in the storage period.
The fungicidal composition of the present invention has a strong activity against various plant pathogenic bacteria, and can exert a strong control effect on the prevention and treatment of plant diseases caused by plant pathogenic bacteria. The fungicidal composition of the present invention has excellent activity against a wide range of phytopathogenic fungi such as Plasmodiophoromycetes, Oomycetes, Chytridiomycetes, Zygomycetes, Ascomycetes, Basidiomycetes, Deuteromycetes, etc.
The bactericidal composition also has very good bactericidal performance and can be used for preventing and controlling plant pathogenic bacteria. Such as pseudomonadaceae, rhizobiaceae, enterobacteriaceae, corynebacteriaceae and streptomycetaceae.
The bactericidal composition can be used as a foliar bactericide in crop protection, and also can be used as a bactericide for seed dressing and as a soil bactericide.
The bactericidal composition can also be used for preventing and treating diseases of fruits and vegetables in the storage period.
The bactericidal composition is suitable for preventing and treating the following phytopathogens:
oomycetes, including Phytophthora (Phytophthora), such as Phytophthora infestans (Phytophthora sojae), Phytophthora sojae (Phytophthora sojae. gaspierma), Phytophthora citrus (Phytophthora parasitica), Phytophthora cinnamomi (Phytophthora cinnamomi), and Phytophthora cucurbitae (Phytophthora capsici); diseases of Pythium species (Pythium), such as Pythium turfgrasum fusarium (Pythium aphanidermatum); and diseases of the Peronosporaceae family (Peronosporae) such as Plasmopara viticola (Plasmoparaviticola); peronospora species (Peronospora) (including Peronospora tabacum (peronosporaabacina) and Peronospora parasitica (Peronospora paralica)); pseudoperonospora species (Pseudoperonospora species) including Pseudoperonospora cubensis (Pseudoperonospora cubensis) and Bremia species (Bremia lactucae), Pythium species (Pythium) such as Pythium aphanidermatum (Pythium aphanidermatum), Rhizoctonia solani (Pythium ultimum), and Plasmopara species (Plasmopara) such as Plasmopara viticola (Plasmopara viticola).
Ascomycetes, including Alternaria (Alternaria) diseases such as early blight of tomato (Alternaria) and black spot of cabbage (Alternaria); globulus (Guignardia) diseases such as botrytis cinerea (Guignardiabidwelli); scabies (Venturia) diseases such as apple scab (Venturia inaequalis); septoria (Septoria) diseases such as glume blight (Septoria nodorum) and leaf blight (Septoria); erysiphe species (Erysiphe) such as Erysiphe graminis (Erysiphe graminis) and Erysiphe graminis (Erysiphe polygonini), Staphylocconospora viticola (Uncinula necatri), Cucumis sativus Erysiphe (Sphaerothecafellugina) and Malaria pumila (Podosphaeraleucotricha); species of pseudocercospora herpotrichoides; diseases of Botrytis species such as Botrytis cinerea (Botrytiscinerea), Monilinia persicae (Moniliniafructicola) diseases; diseases of species of the genus Sclerotinia (sclerotina) such as Sclerotinia sclerotiorum (sclerotina sclerotiorum); pyricularia species (Pyricularia oryzae Cav.) such as Pyricularia oryzae (Magnaporthegrisea); helminthosporium species diseases such as the species alternaria turcicola (Helminthosporium tritici), reticulata (pyrenophores); anthrax (Colletotrichum) species such as Colletotrichum kamuranus (Colletotrichumgraminicola) and Colletotrichum citrulli (Colletotrichum biculare)); gaeumannomyces graminis such as Gaeumannomyces graminis (Gaeumannomyces graminis); genus aphanotheca (Podosphaera); streptozoctonia (Monilinia); devil's claw (Uncinula); mycosphaerella (Mycosphaerella); microsphaeria (Leptosphaeria), Monotheca furcata (Blumeria), Sclerotia (Sclerotia), Potentilla (Gaeumannamyces), Candida (Monilinia).
Basidiomycetes include rust diseases caused by the genus Puccinia (Puccinia) (e.g., Pucciniarecondita (Pucciniarecondita), Pucciniastri (Pucciniastriiformis), Puccinia (Pucciniahordei), Pucciniaria (Pucciniagraminis), and Pucciniarachis (Pucciniaarachidis), Puccinia (Hemiliavastarix), Puccinia (Hemilieia), Puccinia (Phakopsoriacum), Puccinia (Hemiliciariaceae), and Ustilago (Listlinales).
Deuteromycetes, including species of Rhizoctonia (Rhizoctonia), such as Rhizoctonia solani (Rhizoctonia), Rhizoctonia rubescens (Rhizoctonia oryzae), Rhizoctonia solani (Rhizoctonia nilotiensis), Fusarium (Fusarium) diseases, such as Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium oxysporum (Fusarium oxysporum), Fusarium moniliforme (Fusarium proliferatum), Verticillium vulum (Verticillium dahliae), Rhizoctonia solani (Sclerotinfoliii), Phyllospora cloudina (Rhizoctonia oxysporium), Fusarium nigrosporia (Rhizoctonia), such as Fusarium nigrospora (Fusarium oxysporium), Fusarium (Fusarium oxysporium), and Fusarium oxysporium (Fusarium) such as carbophilum); phoma species such as Phoma asparagi (Phomas paragi); septoria such as sheath blight of wheat (Septoria nodorum), Colletotrichum such as Colletotrichum cucurbitacearum (Colletotrichum argentatum); pyricularia species such as Fusarium oxysporum (Pyricularia oryzae), Botrytis species such as Botrytis cinerea (Botrytis cinerea); apple brown spot caused by apple bivalve (Diplocarpon mali Haradaet Sawamura) and apple Panospora piricola (Marssonina coronaria).
The bactericidal composition is particularly suitable for preventing and treating the following phytopathogens: botrytis (Botrytis), Pyricularia (Pyricularia), Helminthosporium (Helminthosporium), Fusarium (Fusarium), Septoria (Septoria), Cercospora (Cercospora), Alternaria (Alternaria), Pyricularia (Pyrococcaria), Pseudocercospora (Pseudocercospora), Rhizoctonia (Rhizoctonia), Puccinia (Heeia), Puccinia (Puccinia), Puccinia (Phakopsora), Ustilago (Listilanalysis), Venturia (Venturia), powdery mildew (Erysiphe), Sphaerotheca (Podosphaera), Neurospora (Monilinia), Uncinula (Uncinula), Mycospora (Mycospora), Phytophora (Phytopora), Plasmopara (Pseudoperonospora).
The fungicidal composition of the present invention is suitable for crops mainly comprising:
cereals (such as rice, barley, wheat, rye, oats, corn, sorghum and the like), legumes (such as soybean, small red bean, broad bean, pea, peanut and the like), fruit trees and fruits (such as apple, citrus, pear, grape, peach, plum or plum, cherry, walnut, apricot, banana, strawberry and the like, and fruits thereof), vegetables (such as cabbage, tomato, spinach, cauliflower, lettuce, onion, welsh onion, pumpkin, cucumber, watermelon, melon and the like), root vegetables (such as carrot, potato, sweet potato, Japanese radish, turnip and the like), industrial crops or crops for industrial processing (such as cotton, hemp, broussonetia, rape, beet, hop, sugarcane, sugar beet, olive tree, rubber tree, coffee tree, tobacco, tea tree and the like), grasses (such as turf grass, corn, pea bean, pea, peanut, and the like), fruit trees and fruits (such as apple, citrus, pear, watermelon, strawberry and the like), fruits, and the, Sorghum, timothy, clover, etc.), turf or turf, spice crops, etc. (e.g., lavender, rosette, thyme, parsley, pepper, ginger, etc.), flowers (e.g., chrysanthemum, rose, orchid, etc.), and the like.
The fungicidal composition of the present invention is preferably used for the prevention and control of, for example, the following plant diseases and the like:
brown spot pathogen (Cercospora betacola), black root pathogen (Aphanomyces cochlioides), root rot pathogen (Thanatephorus cucumeris), leaf rot pathogen (Thanatephorus cucumeris), rhizoctonia solani (Pythium ultium);
brown spot pathogen of "peanut" (Mycosphaerella arachidis), black spot pathogen (mycosphaerelaberkeleyi), root rot pathogen (Thanatephorus cucumeris).
Powdery mildew (Sphaerotheca fuliginea), downy mildew (Pseudoperonospora cubensis), Ralstonia vinelandii (Mycosphaeraella mellonella), Rhizoctonia solani (Fusarium oxysporum), Sclerotium sclerotiorum (Sclerotinia sclerotiorum), Botrytis cinerea (Botrytis cinerea), anthracnose (Colletochytium orbiculare), Cladosporium cucumerinum (Cladosporium cuprinum), Corynespora cassiicola (Corynespora cassiicola), Rhizoctonia solani (Pyroluta debaryana, Rhizoctonia solani kuhn), bacterial leaf spot (Pseudomonas sydoniae pv. lecryans);
botrytis cinerea (Botrytis cinerea), Phytophthora infestans (Cladosporum fulvum), Phytophthora infestans (Phytophthora infestans), Rhizopus oryzae (Thanatephora cucumeri), and Sclerotium Sclerotiorum (Sclerotinia Sclerotiorum) of "tomato";
botrytis cinerea (Botrytis cinerea), Rhizoctonia solani (Corynespora melognae), Erysiphe cichoracearum (Erysiphe cichororaceae), and Mycoselothriella natrassi of eggplant;
botrytis cinerea (Botrytis cinerea), Erysiphe cichoracearum (Sohaerotheca humuli), Colletotrichum (Colletotrichum aculatum), Phytophthora cactorum (Phytophthora cactorum);
"onion" of the species Ceriporia cervicales (Botrytis allii), Botrytis cinerea (Botrytis cinerea), Blastomyces viticola (Botrytis squamosa), Peronospora destructor (Peronospora destructor);
rhizobium (Plasmodiophora brassicae), Rhizoctonia solani (Erwinia arotovora), Peronospora destructor (Peronospora parasitica) of "cabbage";
sclerotinia sclerotiorum (sclerotiorum) and Botrytis cinerea (Botrytis cinerea) of "hyacinth bean";
powdery mildew (Podosphaera leucotricha), scab (Venturia inaqualis), floral rot (Monilinia mali), black spot (Mycosphaerella pomi), rot (Valsa mali), Alternaria maculata (Alternaria mali), Alternaria (Gymnosphaera yamadae), ring spot (Botryosphaera bergiana), anthracnose (Glomella cingula), brown spot (Diplocarpon mali), Mucor flyi (Zygophilia jacensis), sooty mold (Gloeodesporium), leaf spot;
powdery mildew (Phyllantia kakicola), anthrax (Gloeosporium kaki), and alternaria silicea (Cercospora kaki) of "persimmon";
monilinia fructicola (Monilinia fructicola), Cladosporium carophilum (Cladosporium carpophilum), Phomopsis sp.);
monilinia fructicola (Monilinia fructicola) of "cherry";
botrytis cinerea (Botrytis cinerea), Erysiphe cichoracearum (Uncinula necator), Glomeella cingulata (Glomeella cingulata), Colletotrichum acutum (Acutaum), Peronospora destructor (Plasmopavaticola), Blackpox fungus (Elsinoe ampelina), Erysiphe cinerea (Pseudocercospora vitis), and Blackberella auricula (Guignadia bidwellii);
"pear" scab (Venturia nasicola), Alternaria alternata (Gymnosphaera asiatica), Blakeslea scab (Alternaria kikuchiana), Erythrochlomia (Botryosphaeria berengiana), powdery mildew (Phyllantia mali);
the alternaria alternate (Pestalotia theta), anthracnose (Colletotrichum theta-Sinensis) of "tea";
"citrus" scab (Elsinoe fawcetti), Penicillium (Penicillium italicum), chlorophyces (Penicillium digitatum), Botrytis (Botrytis cinerea), alternaria (diaporthri), ulcer (Xanthomonas campestris pv. citri), anthracnose (citrus anthracnose);
"wheat" is selected from powdery mildew (Erysiphe graminis), Fusarium graminearum (Fusarium graminearum), Fusarium culmorum (F. culmorum), Rhizoctonia solani (Microdochium nivale), Rhizoctonia graminis (Puccinia striiformis), Rhizoctonia graminis (P. graminis), Rhizoctonia cerealis (P. recata), Fusarium nivale (Microctria nivale), Rhizoctonia graminis (Ustilago), Rhizoctonia reticulata (Tilletia graminis), Rhizoctonia graminis (Pseudocercospora chrysosporium), Rhizoctonia cerealis (Pseudocercosporella graminis), Rhizoctonia cerealis (Mycosphaera graminis), Rhizoctonia glumae (Stagonorrhoea nodorum), Rhizoctonia graminis (Pyrococcus graminis), Rhizoctonia graminis (Rhizoctonia graminearia solani), and Rhizoctonia graminis (Rhizoctonia graminearia graminis);
powdery mildew of "barley" (Erysiphe graminis), fusarium graminearum (Fusarium graminearum), fusarium avenaceum (F. avenacum), fusarium culmorum (F. culmorum), snow mold leaf blight (Microdochium nivale), wheat stripe rust (Puccinia striiformis), wheat straw rust (P. graminis), barley brown rust (P. hordei), Ustilago nuda (Ustilago nuda), barley cloud germ (Rhynchophylla cocci), barley net blotch germ (Pyrophora teres), barley spot germ (Cochliobolus sativus), barley stripe germ (Pyrophora graminea), and sheath blight germ (Rhizoctonia solani);
"rice" blast (Pyricularia oryzae), sheath blight (Rhizoctonia solani), bakanae disease (Gibberella fujikuroi), flax leaf spot (Cochliobolus niyabenus), Rhizoctonia solani (Pythium graminicola), Rhizoctonia solani (Xanthomonas oryzae), Rhizoctonia solani (Burkholderia plantarii), Monilia (Acidovorax avenae), Rhizoctonia cerealis (Burkholderia glumae), sheath blight (Pyricularia oryzae), stripe bacteria (Cochinobacter miyabenus);
sclerotinia sclerotiorum (sclerotiorum) and Rhizoctonia solani (Rhizoctonia solani) of "rape stalk";
"tobacco" Sclerotinia sclerotiorum (sclerotiniorum), powdery mildew (Erysiphe cichoracearum), Alternaria alternata (Alternaria longipes), powdery mildew (Erysiphe cichoracearum), Colletotrichum orbiculare (Colletotrichum tabacum), Peronospora tabacum (Peronospora tabacum), and Peronospora tabacum (Phytophthora nicotianae);
gray mold of "tulip" (Botrytis cinerea);
snow rot large-grain Sclerotinia (Sclerotinia borealis) and damping-off (pythium aphanidermatum) of zoysia occidentalis;
powdery mildew of "orchard grass" (Erysiphe graminis);
soybean purpura (Cercospora kikuchi), Elsinoe sojae (Elsinoe glycine), Phaseolus phaseoloides (Diaporter phaseolorum var. sojae), Septoria sojae (Septoria glycine), Pseudocercospora sojae (Cercospora sojina), Phoma stigmata (Phakopsora pachyrhizi), Phytophthora sojae (Phytophthora sojae), Rhizoctonia solani (Rhizoctonia solani), Corynospora cassiicola (Corynespora cassicola), Sclerotiella sclerotiorum (Sclerotinia sclerotiorum) of "soybean";
ustilago maydis, Cochliospora heterosporum, Gloecocospora saccharolytica, Puccinia polysora, Cercospora maydis, Rhizoctonia solani;
potato late blight bacteria (Phytophthora infestans), Phytophthora infestans (Phytophthora infestans), and potato powdery scab bacteria (Spongospora subcontanen) of "potato";
pseudoperonospora species (Pseudoperonospora humuli) of the genus Alstonia;
alternaria alternate (Alternaria japonica), white spot pathogen (Cercosporella brassicca), Plasmodiophora brassiccus, and Peronospora parasitica of Cruciferae vegetables;
peronospora destructor (Peronospora destructor);
downy mildew spinach (Peronospora spinosae);
various plant diseases: pythium aphanidermatum (Pythium aphanidermatum), Pythium debianum, Pythium graminearum (Pythium graminicola), Pythium irregulare (Pythium irregularie), Pythium ultimum (Pythium ultimum), Botrytis cinerea (Botrytis cinerea), sclerotinia sclerotiorum (sclerotinia sclerotiorum).
The bactericidal composition is particularly suitable for controlling the following plant diseases: brown spot, alternaria leaf spot, ring spot, anthracnose, scab, and rot on apple; rice sheath blight disease, rice blast, false smut; wheat leaf rust, powdery mildew, leaf blight, glume blight, scab and take-all; grape gray mold, downy mildew, white rot, powdery mildew and spike brown blight; early blight, late blight, black nevus and target spot of potato; barley powdery mildew, net blotch; white gourd downy mildew, anthracnose; early blight, late blight, leaf mold and gray mold of tomato; scab and anthracnose of citrus trees; cucumber scab, gummy stem blight, powdery mildew, gray leaf spot, target disease, downy mildew; anthracnose and epidemic disease of hot pepper; loofah downy mildew; sigatoka, brown spot, scab; powdery mildew of strawberry; powdery mildew of watermelon and gummy stem blight; big leaf spot and small leaf spot of corn; watermelon anthracnose and gummy stem blight; anthracnose of tea trees; brown spot, giant spot and snow rot of lawn; sclerotinia rot of colza; black spot of Chinese cabbage; early leaf spot of peanut; rust disease of soybean; rust disease of jujube trees; coffee rust.
The bactericidal composition of the invention is particularly effective on diseases of the following crops: brown spot, alternaria leaf spot, ring spot, anthracnose, scab, and rot on apple; powdery mildew and gray mold of multiple crops; citrus anthracnose; rice sheath blight disease, rice blast, false smut; black spot of Chinese cabbage; megaspot of lawn; wheat scab; early blight and late blight of tomato.
The bactericidal composition is suitable for preventing and treating the following diseases caused by bacteria: for example, diseases caused by Pseudomonas (Pseudomonas), Xanthomonas (Xanthomonas), Erwinia (Erwinia), and the like.
Examples of diseases caused by bacteria include:
diseases caused by Pseudomonas (Pseudomonas), such as bacterial blight of cucumber (caused by Pseudomonas syringae pv. Iachrymans); bacterial wilt of tomato (caused by Ralstonia solanacearum) of solanaceae; bacterial grain blight (grain rot) of rice plants (caused by Burkholderia glumae);
diseases caused by Xanthomonas (Xanthomonas), such as black rot of cabbage (caused by Xanthomonas campestris (Xanthomonas campestris); bacterial leaf blight of rice plants (caused by flavobacterium solani (Xanthomonas oryzae) and canker of citrus trees (cankar) (caused by citrus canker bacteria (Xanthomonas citri)); diseases caused by Erwinia (Erwinia), such as bacterial soft rot of cabbage (caused by Erwinia carotovora) and the like.
Crops treated with the fungicidal compositions of the present invention are, for example, but not limited to, cereals, legumes, fruit and fruit trees, vegetables, root vegetables, industrial raw material crops or crops for industrial processing, forage, turf or turf, spice crops, flowers and the like.
The bactericidal composition can also be used for preventing and treating diseases of fruits and vegetables in the storage period. For example fruit decay caused by the following pathogens:
aspergillus species, such as Aspergillus flavus;
botrytis (Botrytis) species, such as Botrytis cinerea (Botrytis cinerea);
penicillium (Penicillium) species, such as Penicillium expansum (Penicillium expansum) and p.purpurogenum;
sclerotinia (Sclerotinia) species, such as Sclerotinia (sclerotiorum);
verticillium species, for example Verticillium alboatrum.
The bactericidal composition is also particularly suitable for preventing and treating stalk rot, green mold, penicillium and anthracnose of fruits and vegetables in the storage period.
The fungicidal mixtures according to the invention are also suitable for controlling seed-borne and soil-borne diseases. Such as seed-and soil-borne rot and wilting diseases and seedling diseases caused by the following pathogens:
alternaria species, such as Alternaria brassicolo (Alternaria brassicolo);
species of the genus aphanomyces (aphanomyces), such as, for example, aphanomyces phaseoli (aphanomyces euteiches);
species of the genus Ascochyta (Ascochyta), such as Ascochyta lentis;
aspergillus, such as Aspergillus flavus;
cladosporium species, such as Cladosporium herbarum (Cladosporium herbarum);
species of the genus Sporotrichum, such as Sporotrichum graminum;
anthrax species, such as Colletotrichum fuliginosum (Colletotrichum coccodes);
fusarium species, such as Fusarium flavum;
species of the genus gibberella, such as gibberella zeae;
species of the genus ascochyta (macrophospora), such as ascochyta phaseoloides (macrophosphaaesolina);
the stippled shells belong to species, such as the small stippled shells of snow rot;
penicillium species, such as penicillium expansum;
phaeosphaeria species, such as Phaeosphaeria nodorum;
phoma species, such as Phoma nigra (Phoma linggam);
phomopsis (Phomopsis), such as Phomopsis sojae;
phytophthora species, such as Phytophthora infestans (Phytophthora cacorum);
pyrenophora species, such as Pyrenophora graminea (Pyrenophora graminea);
pyricularia species (Pyricularia) such as Pyricularia oryzae (Pyricularia oryzae);
pythium species, such as pythium ultimum;
rhizoctonia species, such as rhizoctonia solani;
rhizopus species (Rhizopus) such as Rhizopus oryzae (Rhizopus oryzae);
sclerotium species, such as Sclerotium rolfsii;
corallina species (Typhula) such as Scleronaria carolina (Typhula incana);
verticillium species, such as Verticillium dahliae (Verticillium dahliae).
The fungicidal composition of the present invention can be used for the prevention and treatment of various diseases generated when agricultural and horticultural crops including flowers, lawns, pastures, and the like are cultivated by seed treatment, foliage application, soil application, or water surface application.
The invention provides a method for controlling phytopathogenic fungi, by applying said fungicidal composition to the pathogenic fungi and/or to the environment thereof, or to plants, parts of plants, plant propagation material, soil, areas, materials or spaces.
The present invention also provides a method of controlling phytopathogenic fungi of plants, parts of plants, plant propagation material and plant organs which grow at a later time, which comprises applying the fungicidal composition of the invention in an agronomically effective and substantially non-phytotoxic application such as seed treatment, foliar application, stem application, drench, drip, pour, spray, mist, dusting, scattering or smoking, to the plants, parts of plants, plant propagation material or to the soil or to the cultivation medium in which the plants are growing or in which it is desired to grow.
The fungicidal compositions of the present invention are useful for the protection of plant parts, plant propagation material and plant organs that grow at a later time.
The bactericidal composition can treat all plants and plant parts. "plant" means all plants and plant populations such as desirable and undesirable wild plants, cultivars, and plant varieties (whether or not protected by a plant variety or plant cultivar rights-to-human). Cultivated plants and plant varieties may be plants obtained by conventional propagation and cultivation methods, which may be supplemented or supplemented by one or more biotechnological methods, for example using dihaploids, protoplast fusion, random and directed mutations, molecular or genetic markers, or using bioengineering and genetic engineering methods. Plant parts are understood to mean all parts and organs of plants above and below the ground, such as shoots, leaves, flowers and roots, such as leaves, needles, stems, branches, flowers, fruit bodies, fruits and seeds, and also roots, bulbs and rhizomes. Also plants and vegetative and generative propagation material, for example cuttings, bulbs, rhizomes, runners and seeds, belong to the plant part.
The term "plant propagation material" is understood to mean all reproductively competent plant parts, such as seeds, which can be used for the propagation of the latter, and also vegetative materials, such as cuttings or tubers (e.g. potatoes). Thus, plant parts as used herein include plant propagation material. Mention may be made, for example, of seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated plants and useful plants to be inhibited after germination or after emergence from the soil. The young plants can be protected prior to transplantation by a total or partial treatment by dipping.
Preferred propagation material of the present invention is a seed. Examples of the method of seed treatment include a method of diluting a liquid or solid chemical, a method of directly immersing seeds in a liquid solution without dilution to allow the chemical to permeate the seeds, a method of mixing a solid chemical or liquid chemical with seeds to coat the seeds and thereby adhering the chemical to the surfaces of the seeds, and a method of spraying the chemical to the vicinity of the seeds while planting.
A plant part and plant organ that subsequently grows is any part of a plant produced from plant propagation material, such as seeds. Plant parts, plant organs and plants may also benefit from the pathogenic damage protection obtained by applying the fungicidal composition to plant propagation material. Certain plant parts and plant organs that grow after certain locations may also be considered plant propagation material, which itself may be applied (or treated) with the fungicidal composition; thus plants, other plant parts and other plant organs produced from the treated plant parts and treated plant organs may also benefit from the application of the germicidal composition.
Seeds and seedlings are easy to be damaged and infected by various pathogenic bacteria, and are the weakest link in the growth process of crops; the phenomena of diseased seedlings and dead seedlings are caused when seeds and seedlings are infected by pathogenic bacteria, and the effective number of plants of the crops in unit area can be reduced, so that the yield of the crops in large area is reduced, and the quality of the crops is reduced.
The fungicidal compositions according to the invention are suitable for protecting propagation material of plants, such as seeds, fruits, tubers or kernels, or plant cuttings, against infestation by phytopathogens. The propagation material may be treated with the bactericidal composition before application, for example dressing the seed before sowing. The active ingredient may also be applied to the kernel (coating) by soaking the kernel in a liquid composition or coating the kernel with a solid composition. The composition may also be applied to the application site when the propagation material is applied during sowing, for example in a seed sowing trench. These methods of treatment of plant propagation material and the plant propagation material so treated are further subjects of the present invention.
For the treatment of plant propagation material, in particular seeds, it is also possible to impregnate tubers or grains successively with a liquid preparation of the respective active ingredient or to coat them with a combined wet or dry preparation.
The seed treatment application of the invention is carried out by spraying or dusting the seeds before sowing and/or after pregermination.
The fungicidal compositions of the present invention are applied to seeds as such or in a suitable dosage form. Preferably, the seeds are treated in a steady state so that the treatment does not cause any damage. The treatment of the seeds can generally be carried out at any time between harvesting and sowing.
Preferably, the treatment is carried out prior to sowing of the seeds, so that the sown seeds can be pre-treated with the fungicidal composition. In particular, it is preferable to coat the seeds or pelletize the seeds in the treatment of the fungicidal composition of the present invention. As a result of the treatment, the active ingredients in the bactericidal composition adhere to seeds, and thus, can be used for prevention and control of diseases.
The protection of seeds and germinating plants from infestation by phytopathogenic bacteria by treatment of the seeds with seed treatment compositions has long been known and is constantly improving. However, treating seeds also entails a series of problems that cannot always be solved in a satisfactory manner. Therefore, there is a need to develop methods for protecting seeds and germinating plants which avoid the additional application of crop protection products after planting or after emergence of the plants. In addition, it is desirable to optimize the amount of active compound used so that optimum protection is provided to the seed and germinating plant against attack by phytopathogenic fungi without the active compound used being harmful to the plant itself.
However, the undesirable effect is that the use of a seed treatment composition reduces the germination rate of the treated seed. Generally, the germination rate of seeds treated with a seed treatment composition decreases with time after application of the seed treatment agent, thus limiting the shelf life of the treated seeds. Current practice is to time the seed treatment, for example, in the field prior to seed treatment or just prior to planting, to avoid serious damage to the crop. On the other hand, exposure of seeds to environmental stresses such as cold, dryness, etc. may exacerbate the toxic damage that agrochemicals cause to the plants that the seeds produce.
It must generally be noted during seed treatment that the amount of the fungicidal composition of the invention and/or the amount of other additives applied to the seed is selected so as not to affect the germination of the seed or to damage the plant.
The fungicidal compositions of the invention are particularly advantageous for the treatment of plant propagation material, in particular seeds of rice, rape, cotton, wheat, barley, soya, maize, peanuts.
The present invention also provides a method of protecting seeds comprising contacting the seeds before sowing and/or after pregermination with a fungicidal composition according to the invention.
Thus, the present invention also relates inter alia to a method of treating seeds with a fungicidal composition according to the invention in order to protect the seeds and the germinating plants against phytopathogenic fungi.
Furthermore, the present invention relates to the use of the fungicidal compositions according to the invention for the treatment of seeds to protect the seeds and the germinating plants from infestation by phytopathogenic fungi.
Furthermore, the present invention relates to seeds treated with the fungicidal compositions of the present invention to protect against phytopathogenic fungi.
The fungicidal compositions of the present invention may also be used to prevent or control a variety of pathogenic or saprophytic fungi and bacteria in soils or cultivation media. Examples of soil-borne fungal pathogens include Alternaria (Alternaria spp.), Ascochyta (Ascochyta spp.), Botrytis cinerea (Botrytis cinerea), Cercospora (Cercospora spp.), ergot (cladosporium purpurea), Cochliobolus graminis (Cochliobolus sativus), colletotrichum (colletotrichum spp.), epiphora epidothes (Epicoccum spp.), Fusarium graminearum (Fusarium graminearum), Fusarium graminearum (Fusarium moniliforme), Fusarium oxysporum (Fusarium oxysporum), Fusarium graminearum (Fusarium oxysporum), Fusarium oxysporum (rhizoctonum), Fusarium (pyelomyceliophthora), Pyricularia oryzae (Rhizoctonia), Fusarium oxysporum (pyelospora oryzae), Pyricularia oryzae (Rhizoctonia solanum), Pyricularia solanum, Rhizoctonia solanum, Rhizoctonia solanum, Pyricularia (Rhizoctonia solanum, Pyricularia solani, Rhizoctonia solanum, Pyricularia solanum graminea sclerotium, Pyricularia (Rhizoctonia solanum, Pyricularia (Rhizoctonia, Pyricularia solanum, Pyricularia (rhizophora solanum, Pyricularia graminea sclerotium, Pyricularia (rhizophora nivora sp., rhizophora, Pyricularia, ustilago shaft (Sphacelotheca reilliana), Tilletia spp, Sclerotia carolina, Ustilago occulta, Ustilago spp or Verticillium spp.
The soil pathogenic bacteria include Alternaria alternata, Rhizoctonia solani, Fusarium, Phytophthora, damping-off, root rot, Pythium, Botrytis cinerea, Soft rot, etc. Under general conditions, soil pathogenic bacteria can generate a large amount of bacteria, as long as conditions are favorable for growth and development of the pathogenic bacteria and hosts are susceptible, the pathogenic bacteria can propagate in a large amount and infect the hosts, under the host infected with diseases, the pathogenic bacteria can enter a continuous pathogenic period, propagate and diffuse in a large amount along with continuous cropping of crops, but then nutrients are consumed, or when soil conditions such as temperature, humidity and the like are unfavorable for the pathogenic bacteria, the pathogenic bacteria can enter a dormant period. When the host with disease does not exist, soil-borne disease bacteria can survive in soil, and the soil-borne disease bacteria can survive on the root surface or the fallen leaves of the non-host except the soil-borne disease bacteria with wide host range and have the saprophytic competitive ability. However, different pathogenic bacteria are different, such as fusarium and alternaria can survive in soil almost indefinitely.
The culture medium of the present invention refers to a support capable of rooting and growing crops, such as: examples of the raw material include sand, pumice, vermiculite, diatomaceous earth, agar, gel, polymer, asbestos, wood chips, and bark.
Methods of applying agents to soil include methods of spraying a "germicidal composition" onto soil, methods of mixing a "germicidal composition" with soil, and methods of irrigating a "germicidal composition" into soil.
The invention therefore also provides for the use of the fungicidal compositions described for the treatment of the soil in which plants are growing, in order to protect the plants from infestation by pathogenic harmful fungi or bacteria in the soil.
A method of controlling phytopathogenic fungi comprising applying the fungicidal composition of the present invention to plants, plant propagation material and subsequently growing plant organs, cultivation media, materials or spaces in seed treatment, foliar application, stem application, drench, drip, pour, spray, mist, dusting, scattering or smoking at an agronomically effective and substantially non-phytotoxic application rate.
Accordingly, the present invention provides a method for controlling phytopathogenic fungi, which comprises applying the fungicidal composition of the present invention to foliage of a plant.
The present invention provides a method of controlling phytopathogenic fungi comprising applying the fungicidal composition of the present invention to plant propagation material and to plant organs which grow at a later time.
The present invention provides a method for controlling phytopathogenic fungi, which comprises applying the fungicidal composition of the present invention to a cultivation medium.
The invention provides a method of controlling phytopathogenic fungi, which comprises applying said fungicidal composition to plants, plant propagation material and plant organs, cultivation media, materials or spaces which grow at a time before or after the infestation of the plant by the disease.
A method of controlling phytopathogenic fungi comprising the simultaneous or sequential application of benziothiazolinone and difenoconazole.
It is a further object of the present invention to provide a method for controlling phytopathogenic fungi of plants, parts of plants, plant propagation material and plant organs which grow at a later time, a medium in which the plants are cultivated, which comprises applying the fungicidal composition of the present invention to the plants, parts of plants, plant propagation material or to the cultivation medium in which the plants are growing or in which the plants are desired to grow, in an agronomically effective and substantially non-phytotoxic manner by seed treatment, foliar application, stem application, drenching, drip, pouring, spraying, misting, dusting, scattering or fuming, and the like.
The germicidal compositions of the present invention may be applied by various treatment methods, such as:
spraying a liquid comprising the germicidal composition onto the above-ground parts of the plant;
dusting, incorporating granules or powders in the soil, spraying around said plants, and in the case of tree injection or painting;
the seeds of the plants are coated or film-coated.
When the preservative is used for preservation and fresh-keeping of picked fruits and vegetables, the preservative is usually diluted by water with 200-fold and 2000-fold liquid, and the fruits are drained after fruit soaking.
The present invention provides a method for controlling plant pathogenic bacteria, which can be a treatment, prevention or eradication method.
The fungicidal compositions of the present invention are useful for protecting plants against attack by said diseases for a certain period of time after treatment. The period of protection obtained generally extends from 1 to 10 days, preferably from 1 to 7 days, from the time the plant is treated with the composition. Or up to 200 days after treatment of the seed.
The fungicidal composition of the present invention can be used in the form of a conventional chemical preparation, for example, emulsifiable concentrate, wettable powder, suspension, liquid, granule, seed coating, and the like, and the application amount thereof varies depending on the mixing ratio of the active ingredients, weather conditions, the chemical preparation, the application time, the application method, the application site, the control of target pests, the target crop, and the like.
A method for controlling plant pathogenic bacteria comprises simultaneously applying benziothiazolinone and difenoconazole, or applying benziothiazolinone and difenoconazole respectively, or applying benziothiazolinone and difenoconazole sequentially.
The treatment method of the invention may also be used to treat propagation material such as tubers or rhizomes, and may be used to treat seeds, seedlings or transplanted (packing out) seedlings and plants or transplanted plants. This processing method can also be used to process roots. The treatment method of the present invention can also be used for treating the above-ground parts of plants such as the stems, stems or stalks, leaves, flowers and fruits of the plants concerned.
For leaf treatment in general: 0.1 to 10000g/ha, preferably 10 to 1000g/ha, more preferably 50 to 200 g/ha; for dipping or instillation administration, the dosage may even be reduced, particularly when an inert substrate such as asbestos or perlite is applied;
for seed treatment: 2-5000g/100kg of seeds, preferably 3-1000g/100kg of seeds;
for soil or water surface application treatments: 0.1 to 10000g/ha, preferably 1 to 1000 g/ha;
for the preservation of fruits and vegetables after picking, 200 times and 2000 times of liquid can be diluted, and the fruits can be drained after soaking.
The above-mentioned dosages are only typical exemplary dosages, and the person skilled in the art will adjust the application rate in the actual application according to the actual circumstances and needs, in particular according to the nature of the plants or crops to be treated and the severity of the germs.
The fungicidal compositions of the present invention may also be used to protect stored products from pathogen infestation. Suitable examples of such products include stems, leaves, seeds, fruits or grains, which may be protected in the fresh harvest state or during processing, such as pre-drying, moistening, crushing, grinding, extruding or baking.
The bactericidal composition of the invention shows good fungicidal activity, while the fungicidal activity of the single active ingredients is weaker, and the activity of the bactericidal composition of the invention exceeds the simple addition of the activities of the single compounds
The benziothiazolinone of the present invention is applied in combination/association with the difenoconazole. Comprising the separate, sequential or simultaneous application of benziothiazolinone and difenoconazole. Preferably, the benziothiazolinone and difenoconazole are combined in the form of a composition comprising benziothiazolinone and difenoconazole.
The germicidal composition of the invention may be formulated primarily, i.e., the materials in the composition are already mixed, or the components of the composition may be provided in a single dose, mixed in a tank or tank prior to use, and then diluted to the desired concentration. The preparation form provided by the invention is preferably the main form.
As a further improvement of the invention, the bactericidal composition of the invention can be formulated into any agriculturally acceptable dosage form.
As a further improvement of the invention, the formulation of the bactericidal composition of the present invention is aerosol, capsule suspension, cold fogging concentrate, hot fogging concentrate, capsule granule, fine granule, ready-to-use solution, sprayable powder, emulsifiable concentrate, oil-in-water emulsion, water-in-oil emulsion, macro granule, micro granule, oil dispersible powder, oil miscible flowable concentrate, oil miscible liquid, foam, paste, suspension concentrate, soluble concentrate, suspension, seed coating, wettable powder, water dispersible granule, soluble powder, micro capsule suspension, coated granule, extruded granule, emulsifiable concentrate, microemulsion, aqueous emulsion, effervescent tablet, ultra-low volume liquid, suspoemulsion, ultra-low volume cold fogging formulation, ultra-low volume hot fogging formulation, double package (twin pack), dry seed treatment powder, seed treatment emulsion, ultra-low volume hot fogging concentrate, micro emulsion, and the like, Seed treatment suspending agent, seed treatment liquid agent, seed treatment dispersible powder agent, seed treatment microcapsule suspending agent, seed treatment gel, suspoemulsion, emulsion granule, ultra-low volume suspending agent, ultra-low volume liquid agent and dispersible concentrating agent.
The bactericidal composition comprises benziothiazolinone, difenoconazole, a filler and/or a surfactant.
In the bactericidal composition, the sum of the mass of the benziothiazolinone and the mass of the difenoconazole accounts for 5% -90%, more preferably 5% -80%, more preferably 10% -75%, more preferably 10% -70%, more preferably 10% -65%, more preferably 10% -60%, more preferably 10% -55%, more preferably 10% -50%, more preferably 10% -45%, more preferably 10% -40% of the mass of the bactericidal composition.
According to the present invention, the term "filler" refers to a natural or synthetic organic or inorganic compound that can be combined or associated with an active compound to make it easier to apply to a subject (e.g. plants, crops or grasses). Thus, the filler is preferably inert, at least should be agriculturally acceptable. The filler may be solid or liquid.
The inactive medium that can be used in the present invention may be either solid or liquid, and examples of the solid medium material include: examples of the inorganic filler include vegetable powders (for example, particles of soybean powder, starch, cereal powder, wood powder, bark powder, saw dust, walnut shell powder, bran, cellulose powder, coconut shell, corn cob, and tobacco stalk, and residues after extraction of plant essence), paper, saw dust, synthetic polymers such as ground synthetic resins, clays (for example, kaolin, bentonite, and acid china clay), and talc powders. Silica (for example, diatomaceous earth, silica sand, mica, hydrous silicic acid, calcium silicate), activated carbon, natural minerals (for example, pumice, attapulgite, zeolite, etc.), calcined diatomaceous earth, sand, plastic media (for example, polyethylene, polypropylene, polyvinylidene chloride, etc.), inorganic mineral powders such as potassium chloride, calcium carbonate, calcium phosphate, etc., chemical fertilizers such as ammonium sulfate, ammonium phosphate, urea, ammonium chloride, etc., and soil fertilizers, and these may be used alone or in combination of 2 or more.
Examples of the liquid vehicle material that can be used include water, alcohols (e.g., methanol, ethanol, isopropanol, butanol, and ethylene glycol), ketones (e.g., acetone, methyl ethyl ketone, diisobutyl ketone, and cyclohexanone), ethers (e.g., diethyl ether, dioxane, methyl cellulose, and tetrahydrofuran), aliphatic hydrocarbons (e.g., kerosene, and mineral oil), aromatic hydrocarbons (e.g., benzene, toluene, xylene, mineral spirits, alkyl naphthalenes, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, and chlorobenzene), halogenated hydrocarbons, amides, sulfones, dimethyl sulfoxide, mineral and vegetable oils, and animal oils.
Examples of the surfactant that can be used for emulsifying, dispersing, and/or wetting the active ingredient compound include polyacrylic acid salts such as fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid ester, phosphoric acid ester of polyoxyethylene alcohol or phenol, fatty acid ester of polyhydric alcohol, alkaryl sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonate, branched polymer of high molecular comb, butyl naphthalene sulfonate, alkylaryl sulfonate, sodium alkylsulfosuccinate, oils and fats, condensation product of fatty alcohol and ethylene oxide, and alkyltaurate, and protein hydrolysates. Suitable oligosaccharides or polymers are based, for example, on ethylene monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with, for example, (poly) alcohols or (poly) amines.
For dispersion stabilization, attachment and/or binding of the active ingredient compounds, auxiliaries such as xanthan gum, magnesium aluminum silicate, gelatin, starch, cellulose methyl ether, polyvinyl alcohol, polyvinyl acetate and natural phospholipids (such as cephalin and lecithin) as well as synthetic phospholipids, bentonite, sodium lignosulfonate and the like can be used.
Wherein the antifreezing agent can be selected from ethylene glycol, propylene glycol, glycerol, and sorbitol. As the deflocculant for the suspendable product, an auxiliary such as a naphthalenesulfonic acid polymer, a polymeric phosphate, or the like can be used.
The defoaming agent may be a silicone defoaming agent.
Colorants which may be used, for example, inorganic pigments such as iron oxide, titanium oxide and prussian blue; and organic pigments/dyes: alizarin dyes, azo dyes, and metal phthalocyanine dyes; and trace elements such as iron, manganese, boron, copper, cobalt, molybdenum and zinc salts.
Optionally, further additional components, such as protective colloids, binders, thickeners, thixotropic agents, penetrating agents, stabilizers, masking agents, can also be included.
The formulations of the invention can be prepared by mixing the active compounds with the customary additives in a known manner. Such as conventional extenders as well as solvents or diluents, emulsifiers, dispersants, and/or binders or fixatives, wetting agents, water repellents, if desired siccatives and colorants, stabilizers, pigments, defoamers, preservatives, thickeners, water and other processing aids.
The germicidal compositions of the present invention include not only those that are immediately applicable to the subject to be treated by means of a suitable device, such as a spraying or dusting device, but also concentrated commercial compositions that are diluted prior to application to the subject.
The benziothiazolinone-containing and difenoconazole-containing formulations of the invention can also be applied in combination with other active ingredients, for example for expanding the spectrum of activity or for preventing the development of resistance. Such as fungicides, bactericides, attractants, insecticides, acaricides, nematicides, growth regulators, herbicides, safeners, fertilizers or semiochemicals and the like.
The active compounds benziothiazolinone and difenoconazole can be applied simultaneously, or separately, or successively, the order of the separate applications usually having no effect on the control results.
The compositions of the present invention may be presented in a dosage form, i.e., the components of the composition are mixed, or the components of the composition may be provided in a single dose, mixed in a tank or container prior to use, and then diluted to the desired concentration. The preparation form provided by the invention is preferably the main form.
The fungicidal compositions of the invention have improved activity against harmful fungi at a reduced total amount of active compound applied (synergistic). The bactericidal composition of the present invention also has an excellent bactericidal effect against bacteria that exhibit resistance to existing bactericides.
According to the bactericidal composition, the benziothiazolinone and the difenoconazole are subjected to binary compounding, so that the obtained composition has a gain effect on the prevention and treatment effects, the bactericidal spectrum is expanded, the effect of one medicine for multiple purposes is achieved, and the drug resistance of germs is effectively slowed down or avoided. The fungicidal activity of the fungicidal compositions of the invention is significantly higher than the sum of the activities of the individual active compounds, with unpredictable, truly occurring synergistic effects, not just a supplementation of the activities.
The synergistic effect is particularly pronounced when the active compounds are present in the fungicidal compositions of the present invention in a particular weight ratio. However, the weight ratio of the active compounds in the fungicidal compositions according to the invention can vary within certain limits.
Detailed Description
The invention will be further illustrated by the following specific formulation examples.
Formulation examples
Example 1: 27% benziothiazolinone-difenoconazole wettable powder
3% of benziothiazolinone, 24% of difenoconazole, 5% of lignosulfonate, 4% of soapberry powder and 100% of attapulgite, mixing the formula materials, uniformly stirring in a stirring kettle, and uniformly mixing through a jet mill to obtain the 27% benziothiazolinone-difenoconazole wettable powder.
Example 2: 12% benziothiazolinone-difenoconazole wettable powder
2% of benziothiazolinone, 10% of difenoconazole, 6% of naphthalenesulfonic acid formaldehyde condensate, 5% of sodium dodecyl sulfate and 100% of kaolin, mixing the formula materials, uniformly stirring in a stirring kettle, and uniformly mixing through an airflow pulverizer to prepare the benziothiazolinone-difenoconazole wettable powder with the concentration of 12%.
Example 3: 80% benziothiazolinone-difenoconazole wettable powder
8% of benziothiazolinone, 72% of difenoconazole, 5% of modified calcium lignosulfonate, 5% of sodium dodecyl sulfate and 100% of diatomite, mixing the formula materials, uniformly stirring in a stirring kettle, and uniformly mixing through a jet mill to prepare 80% benziothiazolinone-difenoconazole wettable powder.
Example 4: 32% benziothiazolinone/phenylate diazole water dispersible granule
4% of benziothiazolinone, 28% of difenoconazole, 5% of calcium alkylbenzene sulfonate, 5% of sodium dodecyl sulfate, 2% of ammonium sulfate and 100% of bentonite, uniformly mixing the formula materials, kneading the materials by using an ultramicro jet mill, adding the materials into a fluidized bed granulation dryer for granulation, drying and screening, and then sampling, analyzing and mixing the materials to prepare the 32% benziothiazolinone-difenoconazole water dispersible granule.
Example 5: 50% benziothiazolinone-difenoconazole water dispersible granule
5% of benziothiazolinone, 45% of difenoconazole, 5% of fatty acid polyoxyethylene ether, 4% of sodium dodecyl sulfate and 100% of kaolin, uniformly mixing the formula materials, kneading the materials by using an ultramicro jet mill, adding the kneaded materials into a fluidized bed granulation dryer for granulation, drying and screening, and then sampling, analyzing and mixing the materials to prepare the 50% benziothiazolinone-difenoconazole water dispersible granule.
Example 6: 56% benziothiazolinone-difenoconazole water dispersible granule
8% of benziothiazolinone, 48% of difenoconazole, 3% of octylphenol polyoxyethylene ether sulfate, 3% of sodium dodecyl benzene sulfonate, 2% of sodium carbonate and 100% of white carbon black are added, the formula materials are uniformly mixed, kneaded by an ultramicro jet mill, added into a fluidized bed granulation dryer for granulation, drying and screening, and then sampled and analyzed to prepare the 56% benziothiazolinone-difenoconazole water dispersible granule.
Example 7: 40% benzil diazole suspending agent
5% of benziothiazolinone, 35% of difenoconazole, 6% of polycarboxylate, 0.3% of silicone oil, 0.9% of xanthan gum, 2.5% of diethylene glycol and 100% of deionized water, mixing the thickening agent and the antifreezing agent, then uniformly mixing the components except the effective components through high-speed shearing, adding the effective components, and performing ball milling in a ball mill for 2-3 hours to ensure that the particle size is below 5 mu m, thus obtaining the 40% benziothiazolinone-difenoconazole suspending agent.
Example 8: 30% benzil diazole suspending agent
3% of benziothiazolinone, 27% of difenoconazole, 3% of naphthalenesulfonic acid formaldehyde condensate, 0.7% of hydroxyethyl cellulose, 0.1% of silicone compound, 2% of polyethylene glycol and 100% of deionized water, mixing the thickening agent and the antifreezing agent, then uniformly mixing the components except the effective components through high-speed shearing, adding the effective components, and performing ball milling in a ball mill for 2-3 hours to ensure that the particle size is below 5 mu m, thus obtaining the 30% benziothiazolinone-difenoconazole suspending agent.
Example 9: 22% benzil diazole suspending agent
2% of benziothiazolinone, 20% of difenodiconazole, 8% of lignosulfonate, 1% of magnesium aluminum silicate, 0.2% of silicone, 3% of glycerol and 100% of deionized water, mixing the thickening agent and the antifreezing agent, uniformly mixing the components except the effective components through high-speed shearing, adding the effective components, and performing ball milling in a ball mill for 2-3 hours to ensure that the particle size is below 5 mu m, thus obtaining the 22% benziothiazolinone-phenylate diazole suspending agent.
Example 10: 22.5% Thiothiazolinone-phenylate diazole suspoemulsion
2.5% of benziothiazolinone, 20% of difenoconazole, 20% of N-methyl pyrrolidone, 5% of calcium alkyl benzene sulfonate, 1% of methyl cellulose, 3% of propylene glycol, 805% of tween and 100% of water; the finely ground benziothiazolinone suspension is added to the continuous phase containing the benziothiazoline and mixed to produce 22.5% benziothiazolinone-difenoconazole suspoemulsion.
Example 11: 20% benziothiazolinone-difenoconazole microemulsion
2% of benziothiazolinone, 18% of difenoconazole, 5% of cyclohexanone, 4% of ethoxylated castor oil, 5% of calcium dodecylbenzenesulfonate, 20% of N-methylpyrrolidone, 2% of polyethylene glycol, 1.4% of tributyl phosphate and 100% of deionized water, and the 20% benziothiazolinone-difenoconazole microemulsion is prepared.
Example 12: 44% benziothiazolinone-difenoconazole water dispersible granule
4% of benziothiazolinone, 40% of difenoconazole, 5% of octylphenol polyoxyethylene ether sulfate, 3% of sodium dodecyl benzene sulfonate, 1% of sodium carbonate and 100% of white carbon black are added, the formula materials are uniformly mixed, kneaded by an ultramicro jet mill, and then added into a fluidized bed granulation dryer for granulation, drying and screening, and then sampling analysis is carried out, thus obtaining the 44% benziothiazolinone-difenoconazole water dispersible granule.
Example 13: 14% benziothiazolinone-difenoconazole water emulsion
2% of benziothiazolinone, 12% of difenoconazole, 6% of ethoxylated castor oil, 4% of propanol, 3% of calcium dodecyl sulfonate, 0.7% of xanthan gum, 2.4% of propylene glycol, 20% of N, N-dimethylformamide and 100% of deionized water, and the 14% benziothiazolinone-difenoconazole water emulsion is prepared.
Example 14: 9% benzidine emulsifiable concentrate containing benzidine and benzidine
1.5 percent of benziothiazolinone, 7.5 percent of difenoconazole, 3 percent of ethoxylated castor oil, 2 percent of calcium dodecyl benzene sulfonate and 100 percent of N-methyl pyrrolidone are added and stirred until a transparent and uniform phase is obtained, thus obtaining 9 percent of benziothiazolinone-difenoconazole missible oil.
Example 1512% Thiothiazolinone-Difenoconazole suspension seed coating
2% of benziothiazolinone, 10% of difenoconazole, 10% of disodium fatty alcohol polyoxyethylene ether sulfosuccinate monoester, 5% of modified calcium lignosulfonate, 1% of xanthan gum and 1% of bentonite. 5% of glycerol and 301% of PVP-K, and complementing to 100%, uniformly mixing the components in proportion, and sanding to prepare the 12% benziothiazolinone-difenoconazole suspension seed coating agent.
Example 1617.5% Thiothiazolinone-Difenoconazole granulate
Benziothiazolinone 2.5%, difenoconazole 15%, polyethylene glycol 3%, highly dispersed silicic acid 1%, calcium carbonate to 100%, finely ground active ingredient was homogeneously spread on a carrier wetted with polyethylene glycol in a mixer, in this way 17.5% benziothiazolinone-difenoconazole granules were obtained.
Example 17 Thiothiazolinone 10% and difenoconazole 90% were mixed well.
Example 18 Thiothiazolinone 5% and difenoconazole 95% were mixed well.
The proportion of the above embodiment is weight percentage.
Biological test example:
the pesticide prepared by combining the effective components of different pesticides is an effective and quick way for developing and researching new pesticides and preventing and controlling resistant germs in agriculture at present. Pesticides of different species, when mixed, typically exhibit three types of action: additive action, synergistic action and antagonistic action. However, the specific action cannot be predicted, and can only be known through a large number of experiments. The compound synergist has a good formula, and the actual control effect is obviously improved, so that the use amount of the pesticide is reduced, and the generation of resistance is greatly delayed.
Firstly, toxicity test:
calculating the virulence index of each medicament and the cotoxicity coefficient (CTC value) of the mixture by a Sun Yunpei method, wherein when the CTC is less than or equal to 80, the composition shows antagonism, when the CTC is less than 80 and less than 120, the composition shows additivity, and when the CTC is more than or equal to 120, the composition shows synergism.
Observed virulence index (ATI) = (standard agent EC 50/test agent EC50) × 100
Theoretical virulence index (TTI) = a agent virulence index% percentage of a in a mixture + B agent virulence index% percentage of B in a mixture
Co-toxicity coefficient (CTC) = [ actually measured toxicity index (ATI)/Theoretical Toxicity Index (TTI) × 100 for mixed agent)
Test 1: virulence determination of wheat scab
Adopting a method for inhibiting the growth rate of hypha:
dissolving benziothiazolinone and difenoconazole with acetone respectively, diluting with 0.1% tween-80 water solution to prepare liquid medicines with series concentrations, sucking 6mL into a sterilized triangular flask in a super clean bench respectively, adding 54mL of potato glucose agar culture medium (PDA) at about 50 ℃, shaking uniformly, pouring into 4 dishes with the diameter of 9cm, and preparing 4 toxic culture media with corresponding concentrations; the toxic culture medium is prepared by compounding pesticide liquids with different proportions of benziothiazolinone and difenoconazole series concentrations by the same method. The wheat scab germs cultured for 2 days are beaten into fungus blocks at the edges of the colonies by a puncher with the diameter of 5mm, the fungus blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the fungus blocks are cultured in an incubator at 25 ℃, and the treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Figure DEST_PATH_IMAGE004
then, the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun cloud Pepper method.
Table 1: toxicity test result of the invention to wheat scab germ
Reagent for testing Proportioning EC50（PPM） Measured virulence index ATI Theoretical virulence index TTI Co-toxicity coefficient CTC
Benziothiazolinone - 1.76 100 / /
Difenoconazole - 0.23 765.2 / /
Benziothiazolinone: difenoconazole 5:1 0.83 212.72 210.87 100.88
Benziothiazolinone: difenoconazole 1:1 0.33 535.86 432.60 123.87
Benziothiazolinone: difenoconazole 1:5 0.17 1033.72 654.33 157.98
Benziothiazolinone: difenoconazole 1:8 0.14 1225.86 691.29 177.33
Benziothiazolinone: difenoconazole 1:10 0.13 1370.98 704.73 194.54
Benziothiazolinone: difenoconazole 1:12 0.14 1302.68 714.03 182.44
Benziothiazolinone: difenoconazole 1:15 0.14 1247.02 723.63 172.33
Benziothiazolinone: difenoconazole 1:20 0.14 1253.45 733.52 170.88
Benziothiazolinone: difenoconazole 1:25 0.14 1215.41 739.62 164.33
Benziothiazolinone: difenoconazole 1:30 0.15 1161.87 743.74 156.22
Benziothiazolinone: difenoconazole 1:35 0.16 1121.73 746.72 150.22
Benziothiazolinone: difenoconazole 1:40 0.16 1073.43 748.98 143.32
Benziothiazolinone: difenoconazole 1:45 0.17 1051.94 750.74 140.12
Benziothiazolinone: difenoconazole 1:50 0.19 933.50 752.16 124.11
Benziothiazolinone: difenoconazole 1:55 0.22 793.47 753.32 105.33
As can be seen from Table 1, when the weight ratio of the benziothiazolinone to the difenoconazole is in the range of 1:1-1:50 for controlling the wheat scab germs, the co-toxicity coefficients are all larger than 120, which indicates that the benziothiazolinone and the difenoconazole have synergistic effect in the range.
Test 2: virulence determination of apple rot pathogen
Adopting a method for inhibiting the growth rate of hypha:
dissolving benziothiazolinone and difenoconazole with acetone respectively, diluting with 0.1% tween-80 water solution to prepare liquid medicines with series concentrations, sucking 6mL into a sterilized triangular flask in a super clean bench respectively, adding 54mL of potato glucose agar culture medium (PDA) at about 50 ℃, shaking uniformly, pouring into 4 dishes with the diameter of 9cm, and preparing 4 toxic culture media with corresponding concentrations; the toxic culture medium is prepared by compounding pesticide liquids with different proportions of benziothiazolinone and difenoconazole series concentrations by the same method. The apple canker cultured for 2 days is beaten into bacterial blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are placed in an incubator at 25 ℃ for culture, and the treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Figure DEST_PATH_IMAGE005
then, the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun cloud Pepper method.
Table 2: the invention tests the toxicity of apple canker
Reagent for testing Proportioning EC50（PPM） Measured virulence index ATI Theoretical toxicityForce index TTI Co-toxicity coefficient CTC
Benziothiazolinone - 0.94 100 / /
Difenoconazole - 2.75 34.18 / /
Benziothiazolinone: difenoconazole 5:1 1.12 84.17 89.03 94.54
Benziothiazolinone: difenoconazole 1:1 1.14 82.51 67.09 122.98
Benziothiazolinone: difenoconazole 1:5 1.54 61.08 45.15 135.28
Benziothiazolinone: difenoconazole 1:8 1.42 66.29 41.49 159.77
Benziothiazolinone: difenoconazole 1:10 1.25 75.19 40.16 187.21
Benziothiazolinone: difenoconazole 1:12 1.24 75.73 39.24 192.98
Benziothiazolinone: difenoconazole 1:15 1.29 73.05 38.29 190.76
Benziothiazolinone: difenoconazole 1:20 1.51 62.40 37.31 167.24
Benziothiazolinone: difenoconazole 1:25 1.66 56.62 36.71 154.22
Benziothiazolinone: difenoconazole 1:30 1.80 52.11 36.30 143.54
Benziothiazolinone: difenoconazole 1:35 1.94 48.44 36.01 134.53
Benziothiazolinone: difenoconazole 1:40 2.02 46.48 35.79 129.88
Benziothiazolinone: difenoconazole 1:45 2.12 44.43 35.61 124.77
Benziothiazolinone: difenoconazole 1:50 2.17 43.31 35.47 122.11
Benziothiazolinone: difenoconazole 1:55 2.33 40.42 35.36 114.33
As can be seen from Table 2, when the weight ratio of the benziothiazolinone to the difenoconazole is in the range of 1:1-1:50 for controlling the apple rot pathogen, the co-toxicity coefficients are all larger than 120, which indicates that the benziothiazolinone and the difenoconazole have synergistic effect in the range.
Test 3: toxicity determination of apple ring rot pathogen
Adopting a method for inhibiting the growth rate of hypha:
dissolving benziothiazolinone and difenoconazole with acetone respectively, diluting with 0.1% tween-80 water solution to prepare liquid medicines with series concentrations, sucking 6mL into a sterilized triangular flask in a super clean bench respectively, adding 54mL of potato glucose agar culture medium (PDA) at about 50 ℃, shaking uniformly, pouring into 4 dishes with the diameter of 9cm, and preparing 4 toxic culture media with corresponding concentrations; the toxic culture medium is prepared by compounding pesticide liquids with different proportions of benziothiazolinone and difenoconazole series concentrations by the same method. The apple ring rot pathogen cultured for 2 days is beaten into a bacterial block at the edge of a bacterial colony by a puncher with the diameter of 5mm, the bacterial block is transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial block is cultured in an incubator at 25 ℃, and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Figure 317440DEST_PATH_IMAGE005
then, the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun cloud Pepper method.
Table 3: toxicity test result of the invention to apple ring rot pathogen
Reagent for testing Proportioning EC50（PPM） Measured virulence index ATI Theoretical virulence index TTI Co-toxicity coefficient CTC
Benziothiazolinone - 3.87 100 / /
Difenoconazole - 0.44 879.5 / /
Benziothiazolinone: difenoconazole 5:1 1.52 254.15 229.92 110.54
Benziothiazolinone: difenoconazole 1:1 0.64 607.83 489.75 124.11
Benziothiazolinone: difenoconazole 1:5 0.29 1341.60 749.58 178.98
Benziothiazolinone: difenoconazole 1:8 0.26 1486.67 792.89 187.5
Benziothiazolinone: difenoconazole 1:10 0.24 1597.06 808.64 197.5
Benziothiazolinone: difenoconazole 1:12 0.26 1465.33 819.54 178.8
Benziothiazolinone: difenoconazole 1:15 0.28 1399.87 830.78 168.5
Benziothiazolinone: difenoconazole 1:20 0.29 1349.49 842.38 160.2
Benziothiazolinone: difenoconazole 1:25 0.31 1242.00 849.52 146.2
Benziothiazolinone: difenoconazole 1:30 0.32 1197.04 854.35 140.11
Benziothiazolinone: difenoconazole 1:35 0.33 1162.13 857.85 135.47
Benziothiazolinone: difenoconazole 1:40 0.34 1125.52 860.49 130.8
Benziothiazolinone: difenoconazole 1:45 0.35 1105.79 862.55 128.2
Benziothiazolinone: difenoconazole 1:50 0.36 1088.05 864.22 125.9
Benziothiazolinone: difenoconazole 1:55 0.51 757.73 865.58 87.54
As can be seen from Table 3, when the weight ratio of the benziothiazolinone to the difenoconazole is in the range of 1:1-1:50 for preventing and treating the ring rot of apple, the co-toxicity coefficients are all larger than 120, which indicates that the benziothiazolinone and the difenoconazole have synergistic effect in the range.
Test 4: toxicity determination of apple tree scab pathogen
Adopting a method for inhibiting the growth rate of hypha:
dissolving benziothiazolinone and difenoconazole with acetone respectively, diluting with 0.1% tween-80 water solution to prepare liquid medicines with series concentrations, sucking 6mL into a sterilized triangular flask in a super clean bench respectively, adding 54mL of potato glucose agar culture medium (PDA) at about 50 ℃, shaking uniformly, pouring into 4 dishes with the diameter of 9cm, and preparing 4 toxic culture media with corresponding concentrations; the toxic culture medium is prepared by compounding pesticide liquids with different proportions of benziothiazolinone and difenoconazole series concentrations by the same method. The apple tree scab pathogen cultured for 2 days is punched into bacterial blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are placed in an incubator at 25 ℃ for culture, and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Figure 421531DEST_PATH_IMAGE004
then, the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun cloud Pepper method.
Table 4: toxicity test result of apple tree scab pathogen
Reagent for testing Proportioning EC50（PPM） Measured virulence index ATI Theoretical virulence index TTI Co-toxicity coefficient CTC
Benziothiazolinone - 1.225 100 / /
Difenoconazole - 8.235 14.87 / /
Benziothiazolinone: difenoconazole 5:1 1.24 98.87 85.81 115.22
Benziothiazolinone: difenoconazole 1:1 1.70 72.17 57.44 125.65
Benziothiazolinone: difenoconazole 1:5 2.57 47.72 29.06 164.22
Benziothiazolinone: difenoconazole 1:8 2.94 41.60 24.33 170.98
Benziothiazolinone: difenoconazole 1:10 3.02 40.62 22.61 179.65
Benziothiazolinone: difenoconazole 1:12 3.31 37.03 21.42 172.87
Benziothiazolinone: phenylene oxide bisAzole 1:15 3.66 33.49 20.19 165.85
Benziothiazolinone: difenoconazole 1:20 3.98 30.75 18.92 162.47
Benziothiazolinone: difenoconazole 1:25 4.30 28.46 18.14 156.88
Benziothiazolinone: difenoconazole 1:30 4.56 26.86 17.62 152.45
Benziothiazolinone: difenoconazole 1:35 4.73 25.89 17.23 150.22
Benziothiazolinone: difenoconazole 1:40 4.96 24.68 16.95 145.65
Benziothiazolinone: difenoconazole 1:45 5.22 23.45 16.72 140.23
Benziothiazolinone: difenoconazole 1:50 5.95 20.60 16.54 124.55
Benziothiazolinone: difenoconazole 1:55 7.90 15.51 16.39 94.65
As can be seen from Table 4, when the weight ratio of the benziothiazolinone to the difenoconazole is in the range of 1:1-1:50 for controlling apple tree scab, the co-toxicity coefficients are all larger than 120, which indicates that the two compounds have synergistic effect in the range.
Test 5: toxicity determination of rice sheath blight germ
Adopting a method for inhibiting the growth rate of hypha:
dissolving benziothiazolinone and difenoconazole respectively with acetone, and then adopting a method for inhibiting the growth rate of hypha: diluting with 0.1% Tween-80 water solution to obtain medicinal liquids with serial concentrations, respectively sucking 6mL into sterilized Erlenmeyer flasks in a clean bench, adding 54mL of Potato Dextrose Agar (PDA) culture medium at about 50 deg.C, shaking, pouring into 4 dishes with diameter of 9cm, and making into 4 toxic culture media with corresponding concentrations; the toxic culture medium is prepared by compounding pesticide liquids with different proportions of benziothiazolinone and difenoconazole series concentrations by the same method. The rhizoctonia solani cultured for 2 days is beaten into bacterial blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are placed in an incubator at 25 ℃ for culture, and the treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Figure 201268DEST_PATH_IMAGE005
then, the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun cloud Pepper method.
Table 5: the toxicity test result of the invention to rice sheath blight germ
Reagent for testing Proportioning EC50（PPM） Measured virulence index ATI Theoretical virulence index TTI Co-toxicity coefficient CTC
Benziothiazolinone - 0.86 100 / /
Difenoconazole - 3.18 27.04 / /
Benziothiazolinone: difenoconazole 5:1 0.86 99.64 87.84 113.43
Benziothiazolinone: difenoconazole 1:1 1.09 78.97 63.52 124.33
Benziothiazolinone: difenoconazole 1:5 1.40 61.32 39.20 156.44
Benziothiazolinone: difenoconazole 1:8 1.46 59.04 35.15 167.98
Benziothiazolinone: difenoconazole 1:10 1.44 59.56 33.67 176.88
Benziothiazolinone: difenoconazole 1:12 1.35 63.52 32.65 194.54
Benziothiazolinone: difenoconazole 1:15 1.43 60.14 31.60 190.33
Benziothiazolinone: difenoconazole 1:20 1.83 47.06 30.51 154.22
Benziothiazolinone: difenoconazole 1:25 2.01 42.84 29.85 143.53
Benziothiazolinone: difenoconazole 1:30 2.09 41.22 29.39 140.22
Benziothiazolinone: difenoconazole 1:35 2.18 39.37 29.07 135.44
Benziothiazolinone: difenoconazole 1:40 2.29 37.53 28.82 130.22
Benziothiazolinone: difenoconazole 1:45 2.33 36.92 28.63 128.97
Benziothiazolinone: difenoconazole 1:50 2.41 35.71 28.47 125.44
Benziothiazolinone: difenoconazole 1:55 3.18 27.05 28.34 95.44
As can be seen from Table 5, when the weight ratio of the benziothiazolinone to the difenoconazole for preventing and treating rice sheath blight disease is in the range of 1:1-1:50, the co-toxicity coefficients are all larger than 120, which indicates that the benziothiazolinone and the difenoconazole have synergistic effect in the range.
Test 6: virulence determination of rice blast fungus
Adopting a method for inhibiting the growth rate of hypha:
dissolving benziothiazolinone and difenoconazole with acetone respectively, diluting with 0.1% tween-80 water solution to prepare liquid medicines with series concentrations, sucking 6mL into a sterilized triangular flask in a super clean bench respectively, adding 54mL of potato glucose agar culture medium (PDA) at about 50 ℃, shaking uniformly, pouring into 4 dishes with the diameter of 9cm, and preparing 4 toxic culture media with corresponding concentrations; the toxic culture medium is prepared by compounding pesticide liquids with different proportions of benziothiazolinone and difenoconazole series concentrations by the same method. The Pyricularia oryzae cultured for 2 days is beaten into fungus blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the fungus blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the obtained product is cultured in an incubator at 25 ℃, and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Figure 596478DEST_PATH_IMAGE005
then, the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun cloud Pepper method.
Table 6: the toxicity test result of the invention to rice blast germs
Reagent for testing Proportioning EC50（PPM） Measured virulence index ATI Theoretical virulence index TTI Co-toxicity coefficient CTC
Benziothiazolinone - 0.55 100 / /
Difenoconazole - 1.63 33.74 / /
Benziothiazolinone: difenoconazole 5:1 0.59 92.81 88.96 104.33
Benziothiazolinone: difenoconazole 1:1 0.67 82.34 66.87 123.13
Benziothiazolinone: difenoconazole 1:5 0.82 66.72 44.78 148.99
Benziothiazolinone: difenoconazole 1:8 0.85 64.89 41.10 157.87
Benziothiazolinone: difenoconazole 1:10 0.74 74.49 39.76 187.33
Benziothiazolinone: difenoconazole 1:12 0.78 70.20 38.84 180.76
Benziothiazolinone: difenoconazole 1:15 0.84 65.28 37.88 172.33
Benziothiazolinone: difenoconazole 1:20 0.97 56.90 36.90 154.22
Benziothiazolinone: difenoconazole 1:25 1.06 51.65 36.29 142.33
Benziothiazolinone: difenoconazole 1:30 1.13 48.75 35.88 135.87
Benziothiazolinone: difenoconazole 1:35 1.19 46.32 35.58 130.19
Benziothiazolinone: difenoconazole 1:40 1.23 44.82 35.36 126.77
Benziothiazolinone: difenoconazole 1:45 1.26 43.78 35.18 124.44
Benziothiazolinone: difenoconazole 1:50 1.30 42.39 35.04 120.99
Benziothiazolinone: difenoconazole 1:55 1.70 32.28 34.92 92.43
As can be seen from Table 6, when the weight ratio of benziothiazolinone to difenoconazole is in the range of 1:1-1:50 for preventing and treating Pyricularia oryzae, the co-toxicity coefficients are all larger than 120, which indicates that the benziothiazolinone and difenoconazole have synergistic effect in the range.
Test 7: toxicity determination of apple alternaria leaf spot
Adopting a method for inhibiting the growth rate of hypha:
dissolving benziothiazolinone and difenoconazole with acetone respectively, diluting with 0.1% tween-80 water solution to prepare liquid medicines with series concentrations, sucking 6mL into a sterilized triangular flask in a super clean bench respectively, adding 54mL of potato glucose agar culture medium (PDA) at about 50 ℃, shaking uniformly, pouring into 4 dishes with the diameter of 9cm, and preparing 4 toxic culture media with corresponding concentrations; the toxic culture medium is prepared by compounding pesticide liquids with different proportions of benziothiazolinone and difenoconazole series concentrations by the same method. The alternaria mali cultured for 2 days is beaten into bacterial blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are cultured in an incubator at 25 ℃, and the treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Figure 82954DEST_PATH_IMAGE005
then, the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun cloud Pepper method.
Table 7: toxicity test result of the invention to alternaria mali
Reagent for testing Proportioning EC50（PPM） Measured virulence index ATI Theoretical virulence index TTI Co-toxicity coefficient CTC
Benziothiazolinone - 5.784 100 / /
Difenoconazole - 1.998 289.4 / /
Benziothiazolinone: difenoconazole 5:1 4.82 120.02 131.57 91.22
Benziothiazolinone: difenoconazole 1:1 2.36 244.64 194.70 125.65
Benziothiazolinone: difenoconazole 1:5 1.25 461.47 257.83 178.98
Benziothiazolinone: difenoconazole 1:8 1.15 503.30 268.36 187.55
Benziothiazolinone: difenoconazole 1:10 1.08 537.70 272.18 197.55
Benziothiazolinone: difenoconazole 1:12 1.11 523.39 274.83 190.44
Benziothiazolinone:difenoconazole 1:15 1.22 473.38 277.56 170.55
Benziothiazolinone: difenoconazole 1:20 1.32 439.22 280.38 156.65
Benziothiazolinone: difenoconazole 1:25 1.46 396.51 282.12 140.55
Benziothiazolinone: difenoconazole 1:30 1.52 380.29 283.29 134.24
Benziothiazolinone: difenoconazole 1:35 1.62 357.65 284.14 125.87
Benziothiazolinone:difenoconazole 1:40 1.67 346.44 284.78 121.65
Benziothiazolinone: difenoconazole 1:45 1.68 343.91 285.28 120.55
Benziothiazolinone: difenoconazole 1:50 1.69 343.19 285.69 120.13
Benziothiazolinone: difenoconazole 1:55 2.23 258.99 286.02 90.55
As can be seen from Table 7, when the weight ratio of the benziothiazolinone to the difenoconazole is in the range of 1:1-1:50 for controlling alternaria mali, the co-toxicity coefficients are all larger than 120, which indicates that the benziothiazolinone and the difenoconazole have synergistic effect in the range.
Test 8: toxicity determination of citrus anthracnose pathogen
Adopting a method for inhibiting the growth rate of hypha:
dissolving benziothiazolinone and difenoconazole with acetone respectively, diluting with 0.1% tween-80 water solution to prepare liquid medicines with series concentrations, sucking 6mL into a sterilized triangular flask in a super clean bench respectively, adding 54mL of potato glucose agar culture medium (PDA) at about 50 ℃, shaking uniformly, pouring into 4 dishes with the diameter of 9cm, and preparing 4 toxic culture media with corresponding concentrations; the toxic culture medium is prepared by compounding pesticide liquids with different proportions of benziothiazolinone and difenoconazole series concentrations by the same method. The citrus anthracnose pathogen cultured for 2 days is beaten into bacterial blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are placed in an incubator at 25 ℃ for culture, and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
then, the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun cloud Pepper method.
Table 8: toxicity test result of the invention to citrus anthracnose pathogen
Reagent for testing Proportioning EC50（PPM） Measured virulence index ATI Theoretical virulence index TTI Co-toxicity coefficient CTC
Benziothiazolinone - 0.85 100 / /
Difenoconazole - 0.46 185.22 / /
Benziothiazolinone: difenoconazole 5:1 0.71 120.54 114.20 105.55
Benziothiazolinone: difenoconazole 1:1 0.50 171.33 142.61 120.14
Benziothiazolinone: difenoconazole 1:5 0.30 287.10 171.02 167.88
Benziothiazolinone: difenoconazole 1:8 0.27 314.96 175.75 179.21
Benziothiazolinone: difenoconazole 1:10 0.28 302.68 177.47 170.55
Benziothiazolinone: difenoconazole 1:12 0.29 296.37 178.66 165.88
Benziothiazolinone: difenoconazole 1:15 0.29 289.23 179.89 160.78
Benziothiazolinone: difenoconazole 1:20 0.33 254.01 181.16 140.21
Benziothiazolinone: difenoconazole 1:25 0.36 237.58 181.94 130.58
Benziothiazolinone: difenoconazole 1:30 0.37 229.88 182.47 125.98
Benziothiazolinone: difenoconazole 1:35 0.37 228.16 182.85 124.78
Benziothiazolinone: difenoconazole 1:40 0.37 226.86 183.14 123.87
Benziothiazolinone: difenoconazole 1:45 0.38 224.00 183.37 122.16
Benziothiazolinone: difenoconazole 1:50 0.38 221.87 183.55 120.88
Benziothiazolinone: difenoconazole 1:55 0.54 156.53 183.70 85.21
As can be seen from Table 8, when the weight ratio of the benziothyron to the benzidine is 1:1-1:50 for controlling the citrus anthracnose pathogen, the cotoxicity coefficient is greater than 120, which indicates that the benziothyrone and the benzidine both have synergistic effect in the range.

Claims (10)

1. A germicidal composition, characterized by: the bactericidal composition comprises the active ingredients of benziothiazolinone and difenoconazole, wherein the weight ratio of the benziothiazolinone to the difenoconazole is 1:1-1:50, preferably 1:5-1:40, preferably 1:5-1:35, more preferably 1:5-1:30, more preferably 1:5-1:25, more preferably 1:5-1:20, and more preferably 1:8-1: 20.
2. The germicidal composition of claim 1, wherein: the sum of the mass of the benziothiazolinone and the mass of the difenoconazole accounts for 5% -90%, more preferably 5% -80%, more preferably 10% -75%, more preferably 10% -70%, more preferably 10% -65%, more preferably 10% -60%, more preferably 10% -55%, more preferably 10% -50% of the mass of the sterilization composition.
3. The germicidal composition of claim 1, wherein: the bactericidal composition is in the dosage form of a suspending agent, a seed treatment suspending agent, a suspoemulsion, a wettable powder, a water dispersible granule, a microcapsule suspending agent, a granule, missible oil, a microemulsion, an emulsion in water, an effervescent tablet, an ultra-low volume liquid, a hot fogging concentrate, a dry suspending agent and an oil suspending agent.
4. The germicidal composition of claim 1, wherein: also comprises a filler and/or a surfactant.
5. Use of the fungicidal compositions according to claim 1 for controlling fungi and bacteria on cereals, legumes, fruit and fruits, vegetables, root vegetables, forage grasses, turf grass or turf, spice crops, flowering plants.
6. The sterilization composition of claim 1 is used for preventing and treating brown spot, alternaria leaf spot, ring spot, anthracnose, scab and rot of apples; powdery mildew and gray mold of multiple crops; rice sheath blight disease, rice blast, false smut; wheat scab; application of citrus anthracnose is provided.
7. Use of the fungicidal composition according to claim 1 for the treatment of seeds to protect the seeds from fungal and bacterial attack.
8. Use of the fungicidal composition according to claim 1 for treating soil where plants grow to protect the plants from fungal and bacterial attack in the soil.
9. A method of controlling phytopathogenic fungi, characterized in that a fungicidal composition according to claim 1 is applied to plants, plant propagation material and plant organs, cultivation media, materials or spaces which grow at a later time, before or after the infestation of the plants by the diseases.
10. The method of claim 9, wherein: the fungicidal composition of claim 1 is applied to a plant, plant propagation material and subsequently emerging plant organs, cultivation media, material or space in an agronomically effective and substantially non-phytotoxic application rate by seed treatment, foliar application, stem application, drench, drip, pour, spray, dusting, scattering or fuming.
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Publication number Priority date Publication date Assignee Title
CN114946867A (en) * 2020-12-31 2022-08-30 江苏省农业科学院 Method for promoting rice root growth by using benziothiazolinone

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CN1923819A (en) * 2006-10-12 2007-03-07 青岛科技大学 compound containing aromatic ether and ditriazole and use thereof
CN102258037A (en) * 2011-05-25 2011-11-30 陕西西大华特科技实业有限公司 Sterilizing composition containing benziothiazolinone and tebuconazole

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN1923819A (en) * 2006-10-12 2007-03-07 青岛科技大学 compound containing aromatic ether and ditriazole and use thereof
CN102258037A (en) * 2011-05-25 2011-11-30 陕西西大华特科技实业有限公司 Sterilizing composition containing benziothiazolinone and tebuconazole

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114946867A (en) * 2020-12-31 2022-08-30 江苏省农业科学院 Method for promoting rice root growth by using benziothiazolinone
CN114946867B (en) * 2020-12-31 2023-06-16 江苏省农业科学院 Method for promoting rice root growth by using benziothiazolinone

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