CN108260593B - Bactericidal composition - Google Patents
Bactericidal composition Download PDFInfo
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- CN108260593B CN108260593B CN201611258113.7A CN201611258113A CN108260593B CN 108260593 B CN108260593 B CN 108260593B CN 201611258113 A CN201611258113 A CN 201611258113A CN 108260593 B CN108260593 B CN 108260593B
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- propiconazole
- pyrimethanil
- composition
- fungi
- bactericidal composition
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
Abstract
The invention relates to a bactericidal composition and also relates to a method for preventing and treating plant pathogenic bacteria; in particular to a method for preventing and controlling fungal and bacterial diseases of cereals, fruits and vegetables in the storage period. The active ingredients of the bactericidal composition consist of propiconazole and pyrimethanil, and the weight ratio of the propiconazole to the pyrimethanil is 10:1-1: 50. The invention also relates to the application of the bactericidal composition in preventing and controlling fungi and bacteria on cereals, vegetables, fruits, ornamental plants and grapevines. The invention relates in particular to the use of the fungicidal compositions for protecting fruit or vegetable stocks against fungal or bacterial infestation during storage.
Description
Technical Field
The invention relates to a bactericidal composition and also relates to a method for preventing and treating plant pathogenic bacteria; in particular to a method for preventing and controlling fungal and bacterial diseases of cereals, fruits and vegetables in the storage period.
Background
With regard to the activity of pesticides, in particular with regard to crop protection, one of the core problems of the research carried out in this technical field is the improvement of the properties, in particular in terms of biological activity, and the maintenance of this activity over a certain period of time.
Pyrimethanil (Pyrimethanil) is a pyrimidinamine based fungicide developed by egeft, germany. The pyrimethanil can be used for preventing and treating gray mold of cucumber, tomato, grape, strawberry, pea, leek and other crops. Can also be used for preventing and treating pear scab, apple scab and alternaria leaf spot.
Propiconazole (Propiconazole) is a triazole fungicide developed by shindak corporation. Propiconazole can be absorbed by roots, stems and leaves, and can be quickly conducted upwards in plants. Propiconazole has been described in US 4079062; the chemical structure is as follows:
it is an object of the present invention to provide compositions having improved activity against harmful fungi with a reduced total amount of active compounds applied (synergistic compositions), in order to reduce the application rates of known active compounds and to improve their activity spectrum.
Disclosure of Invention
The object of the present invention is to provide fungicidal compositions which have an improved activity against harmful fungi at a reduced total amount of active compounds applied (synergism), in terms of reduced application rates and an improved activity profile of the known compounds propiconazole and pyrimethanil.
We have found that the simultaneous, i.e. joint or separate, administration of propiconazole and pyrimethanil, or the sequential administration of propiconazole and pyrimethanil, allows better control of harmful fungi than the individual compounds administered alone.
The invention provides a bactericidal composition, which is prepared by binary compounding of propiconazole and pyrimethanil, so that the obtained composition has a gain effect on the prevention and treatment effects, the bactericidal spectrum is expanded, the effect of one medicine for multiple purposes is achieved, and the generation of drug resistance of germs is effectively slowed down or avoided. Surprisingly, the fungicidal activity of the fungicidal compositions according to the invention is significantly higher than the sum of the activities of the individual active compounds. In other words, there is an unpredictable, truly present synergistic effect, not just a supplementation of activity.
Through various intensive researches, the invention discovers that the bactericidal composition containing the propiconazole and the pyrimethanil can control the fungal and bacterial diseases of various vegetables, fruits, fruit trees and cereal crops. The composition containing propiconazole and pyrimethanil is very effective not only against common plant pathogens but also against pathogens which have developed drug resistance, and has excellent control effects even under conditions where diseases continue to develop, thus completing the present invention.
The synergistic effect is particularly pronounced when the active compounds are present in the fungicidal compositions of the present invention in a particular weight ratio. However, the weight ratio of the active compounds in the fungicidal composition may vary within certain limits.
The bactericidal composition provided by the invention has a synergistic effect and is suitable for preventing and treating harmful fungi and bacteria. And the bactericidal composition is especially suitable for controlling fungal and bacterial diseases in cereals, vegetables, fruits, ornamental plants and grapevines.
The bactericidal composition is particularly suitable for preventing and treating fungal and bacterial diseases of cereals, fruits and vegetables in the storage period.
The sterilization composition is realized by adopting the following technical scheme:
the bactericidal composition comprises the active ingredients of propiconazole and pyrimethanil in a weight ratio of 10:1-1:50, preferably 5:1-1:25, more preferably 5:1-1:20, further preferably 1:1-1:15, more preferably 1:5-1: 15.
Propiconazole and pyrimethanil may be administered simultaneously, i.e. together or separately, or sequentially; the sequence in the case of separate application generally has no effect on the results of the control measures.
The active ingredients propiconazole and pyrimethanil may also be 10:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,1:15,1:16,1:17,1:18,1:19,1:20,1:21,1:22,1:23,1:24,1:25,1:26,1:17,1:28,1:29,1:30,1:31,1:32,1:33,1:34,1:35,1:36,1:37,1:38,1:39,1:40,1:41,1:42,1: 44,1: 46,1: 48,1: 47,1:48,1: 8,1:9,1:10,1: 23.
The bactericidal composition may contain only the active ingredient, or may further contain a filler and/or a surfactant.
The dosage form of the bactericidal composition is wettable powder, missible oil, suspending agent, suspoemulsion, microcapsule, seed coating agent, microemulsion, aqueous emulsion, water dispersible granule, foaming agent, paste, aerosol, ultra-low volume liquid, ultra-low volume powder, granule and effervescent tablet.
The bactericidal composition contains 1% -90%, preferably 5% -80% and more preferably 10% -60% of the active ingredients of propiconazole and pyrimethanil.
The content of the active ingredients of the bactericidal composition, namely the propiconazole and the pyrimethanil, can also be 1%, 3%, 5%,7%,8%,9%,10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% of the bactericidal composition.
The bactericidal composition is used for preventing and controlling fungi and bacteria on cereals, vegetables, fruits, ornamental plants and grapevines.
The bactericidal composition is used for preventing and treating leaf spot disease, root rot, downy mildew, penicilliosis, green mildew, gray mold, sclerotinia, brown rot, leaf spot disease, banded sclerotial blight, take-all, rust disease, anthracnose, early blight, powdery mildew, scab, black spot, gummy stem blight, alternaria leaf spot, acid rot, crown rot, soft rot, mucormycosis, black spot, black rot, epidemic disease and sclerotinia on cereals, fruits, vegetables, cotton, fruit trees and ornamental plants.
The bactericidal composition is used for preventing and treating diseases of fruits or vegetables in the storage period.
The use of the fungicidal composition for protecting fruit or vegetable stock products from fungal or bacterial infestation during storage.
The bactericidal composition is used for preventing and treating acid rot, penicilliosis, green mold, black rot, stalk rot, scorch, anthracnose, crown rot, frost blight, gray mold, brown rot, soft rot, mucormycosis, brown scald, bitter pox, water core disease and black core disease of fruits and vegetables during storage.
The bactericidal composition is used for preventing and treating citrus storage period diseases.
The bactericidal composition is used for preventing and treating anthracnose, acid rot, penicilliosis, green mold and scorch of oranges in the storage period.
A method for preventing and treating plant pathogenic bacteria comprises applying bactericidal composition to pathogenic bacteria and/or its environment, or plant, seed, soil, material or space, and storage.
A method for preventing and treating plant pathogenic bacteria comprises applying the bactericidal composition to soil or culture medium.
A method for controlling phytopathogenic fungi comprises simultaneously or separately applying propiconazole and pyrimethanil to the pathogenic fungi and/or their environment, or to the plants, seeds, soil, materials or spaces, storage.
A method for controlling phytopathogenic fungi comprises applying a fungicidal composition to the pathogenic fungi and/or their environment, or to the plants, seeds, soils, materials or spaces, stores, by seed treatment, foliar application, stem application, drenching, instillation, casting, spraying, dusting, spreading or smoking.
A method of protecting a fruit or vegetable stock from fungal or bacterial infestation during storage comprising contacting the stock with a germicidal composition of the invention.
A method of protecting a fruit or vegetable stock from fungal or bacterial infestation during storage, comprising: (i) before harvesting; (ii) after harvesting; or (i) and (ii), contacting the germicidal composition of the present invention with the fruit or vegetable.
Detailed Description
The invention aims to provide a bactericidal composition, and active ingredients of the bactericidal composition consist of propiconazole and pyrimethanil. The synergistic effect of propiconazole and pyrimethanil, the weight ratio of propiconazole to pyrimethanil can be selected in a relatively large range.
The weight ratio of propiconazole to pyrimethanil is 10:1 to 1:50, more preferably 5:1 to 1:25, more preferably 5:1 to 1:20, still more preferably 1:1 to 1:15, more preferably 1:5 to 1: 15.
The weight ratio of the active ingredients of the propiconazole to the pyrimethanil can also be as follows: 10:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,1:15,1:16,1:17,1:18,1:19,1:20,1:21,1:22,1:23,1:24,1:25,1:26,1:17,1:28,1:29,1:30,1:31,1:32,1:33,1:34,1:35,1:36,1:37,1:38,1:39,1:40,1:41,1:42,1:43,1:44,1:45,1:46,1:47,1:48,1:49,1:50.
The content of the active ingredients of the bactericidal composition depends on the dosage of the bactericide when used alone, and also depends on the mixing proportion of one bactericide and another bactericide and the degree of synergism.
The bactericidal composition contains 1% -90%, preferably 5% -80% and more preferably 10% -60% of the active ingredients of propiconazole and pyrimethanil.
The content of the active ingredients of the bactericidal composition, namely the propiconazole and the pyrimethanil, can also be 1%, 3%, 5%,7%,8%,9%,10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% of the bactericidal composition.
The bactericidal composition may contain only the active ingredient, or may further contain a filler and/or a surfactant.
The bactericidal composition can be prepared into various formulations, such as wettable powder, missible oil, suspending agent, suspending emulsion, microcapsule, seed coating agent, microemulsion, aqueous emulsion, water dispersible granule, foaming agent, paste, aerosol and ultra-low volume spray solution.
According to the present invention, the term "filler" refers to a natural or synthetic organic or inorganic compound that can be combined or associated with an active compound to make it easier to apply to a subject (e.g. plants, crops or grasses). Thus, the filler is preferably inert, at least should be agriculturally acceptable. The filler may be solid or liquid.
Liquid fillers are typically: water, alcohols (e.g., methanol, ethanol, isopropanol, butanol, ethylene glycol, etc.), ketones (e.g., acetone, methyl ethyl ketone, diisobutyl ketone, cyclohexanone, etc.), ethers (e.g., diethyl ether, dioxane, methyl cellulose, tetrahydrofuran, etc.), aliphatic hydrocarbons (e.g., kerosene, mineral oil, etc.), aromatic hydrocarbons (e.g., benzene, toluene, xylene, mineral spirits, alkyl naphthalenes, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, chlorobenzene, etc.), halogenated hydrocarbons, amides, sulfones, dimethyl sulfoxide, mineral and vegetable oils, animal oils, etc.
Solid fillers are typically: examples of the inorganic filler include vegetable powders (for example, particles of soybean powder, starch, cereal powder, wood powder, bark powder, saw dust, walnut shell powder, bran, cellulose powder, coconut shell, corn cob, and tobacco stalk, and residues after extraction of plant essence), paper, saw dust, synthetic polymers such as ground synthetic resins, clays (for example, kaolin, bentonite, and acid china clay), and talc powders. Silica (for example, diatomaceous earth, silica sand, mica, hydrous silicic acid, calcium silicate), activated carbon, natural minerals (for example, pumice, attapulgite, zeolite, etc.), calcined diatomaceous earth, sand, plastic media (for example, polyethylene, polypropylene, polyvinylidene chloride, etc.), inorganic mineral powders such as potassium chloride, calcium carbonate, calcium phosphate, etc., chemical fertilizers such as ammonium sulfate, ammonium phosphate, urea, greening ammonium, etc., and soil fertilizers, and these may be used alone or in combination of 2 or more.
Examples of the surfactant that can be used for emulsifying, dispersing, solubilizing, and/or wetting the active ingredient compound include polyacrylic acid salts such as fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid ester, phosphoric acid ester of polyoxyethylene alcohol or phenol, fatty acid ester of polyhydric alcohol, alkylaryl sulfonic acid, naphthalenesulfonic acid polymer, lignosulfonate, branched polymer of high molecular comb, butylnaphthalenesulfonate, alkylaryl sulfonate, sodium alkylsulfosuccinate, fats and oils, condensates of fatty alcohol and ethylene oxide, and alkyltaurates, and protein hydrolysates. Suitable oligosaccharides or polymers are based, for example, on ethylene monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with, for example, (poly) alcohols or (poly) amines.
Tackifiers such as carboxymethylcellulose and natural and synthetic polymers in the form of powders, granules or latices, such as gum arabic, polyvinyl alcohol and polyvinyl acetate, or else natural phospholipids, such as cephalins and lecithins, and synthetic phospholipids, can also be used in the formulations. Other additives are mineral oils and vegetable oils.
It is possible to use colorants such as inorganic pigments, for example iron oxide, titanium oxide and Prussian blue, and organic dyes, such as alizarin dyes, azo dyes or metal phthalocyanine dyes, and trace nutrients, such as salts of iron, manganese, boron, ketones, cobalt, molybdenum and zinc.
Disintegrants which may be used are selected from: one or more of bentonite, urea, ammonium sulfate, aluminum chloride, citric acid, succinic acid and sodium bicarbonate.
Stabilizers which may be used are selected from: one of sodium citrate and resorcinol.
The antifreeze agents which may be used are selected from: one or more of ethylene glycol, propylene glycol, glycerol and urea.
The defoaming agent is selected from: silicone oil, Silicone Compound, C10-20Saturated fatty acid compound, C8-10One or more of fatty alcohol compounds.
Optionally, further additional components, such as protective colloids, binders, thickeners, thixotropic agents, penetrating agents, stabilizers, masking agents, can also be included.
The formulations of the invention can be prepared by mixing the active ingredients with fillers and/or surfactants in a known manner, it being possible for further conventional additives, such as siccatives and colorants, stabilizers, pigments, defoamers, preservatives, thickeners, etc., to be added.
The fungicidal compositions of the invention can also be used in combination with other agents having herbicidal, insecticidal or fungicidal properties, in particular with protective fungicides, and also with insecticides, protectants, growth regulators, plant nutrients or soil conditioners.
The fungicidal composition of the present invention has a strong activity against various plant pathogenic bacteria, and can exert a strong control effect on the prevention and treatment of plant diseases caused by plant pathogenic bacteria.
The fungicidal composition of the present invention has excellent activity against a wide range of phytopathogenic fungi such as Plasmodiophoromycetes, Oomycetes, Chytridiomycetes, Zygomycetes, Ascomycetes, Basidiomycetes, Deuteromycetes, Phycomycetes, etc.
The bactericidal composition also has very good bactericidal performance and can be used for preventing and controlling plant pathogenic bacteria. Such as pseudomonadaceae, rhizobiaceae, enterobacteriaceae, corynebacteriaceae and streptomycetaceae.
The bactericidal composition is used for preventing and controlling fungi and bacteria on cereals, vegetables, fruits, ornamental plants and grapevines.
The bactericidal composition is suitable for preventing and treating the following phytopathogens:
the subclasses Oomycetes can be exemplified by, for example, Pythium species such as Rhizoctonia solani (Pythium ultium) of various crops; phytophthora species such as Phytophthora solani (Phytophthora infestans), Botrytis cinerea (Phytophthora capsici); pseudoperonospora species such as Pseudoperonospora cubensis (Pseudoperonospora cubensis), peronospora humuli (Pseudoperonospora humuli); plasmopara species such as Plasmopara viticola; such as Peronospora brassiccus (Peronospora brassicae), Peronospora shallot (Peronospora destructor), Peronospora spinacia (Peronospora spinosae), and the like.
Ascomycetes, for example, Erysiphe species such as Erysiphe graminis (Erysiphe); sphaerotheca species such as vegetable powdery mildew (Sphaerotheca fuliginea); venturia species such as apple scab (Venturia aequalis), pear scab (Venturia nashi cola); pyrenophora species such as barley reticulum (Pyrenophorates); a bacterium belonging to the genus Cochliobolus such as barley speckled disease (Cochliobolus sativus); such as Sclerotinia sclerotiorum (sclerotiorum) of vegetable.
Basidiomycetes, for example, can be exemplified by, for example, Puccinia such as Pucciniarodidonita; tilletia species such as Tilletia foetida (Tilletiaciaries); such as Ustilago sp.of barley loose smut (Ustilago).
Deuteromycotina, for example, a strain of the genus Phoma, such as, for example, Phoma asparagi (Phoma asparagi); septoria such as sheath blight of wheat (Septoria nodorum); colletotrichum species such as Colletotrichum lagenarium (Colletotrichum lagenarium); pyricularia species such as Fusarium oxysporum (Pyricularia oryzae); botrytis cinerea such as Botrytis cinerea; alternaria species such as Alternaria mali (Alternaria mali) and Alternaria solani (Alternaria solani); cercospora species such as Cercospora (Cercospora betacola); a species of the genus amycolata such as the species Cladosporium persicinum (Cladosporium carpophilum); such as Rhizoctonia species of Rhizoctonia solani (Rhizoctonia solani).
The fungicidal compositions according to the invention are particularly suitable for controlling the following phytopathogenic fungi:
erysiphe graminis (Erysiphe graminis) on cereals, Erysiphe graminis (Erysiphe graminearum) and Xanthium strumarium (Sphaerotheca fuliginea), Erysiphe graminis (Podosphaera leucotricha) on apples, Uncinula viticola (Uncinula necator) on grapes, Puccinia graminis (Puccinia) on cereals, Rhizoctonia solani (Rhizoctonia solani) on cotton, rice and lawn, Ustilago on cereals and sugarcane, Venturia inaequalis (Venturia inaequalis) on apples, Helminthosporium graminis (Helminthosporium) on cereals, Rhizoctonia glumae (Septorium) on wheat, strawberry, vegetable, Botrytis cinerea (Botrysinea) on ornamental plants and grapes, Arachida arachidicola (Helicoides) on peanuts, Pseudoceria graminis (Ceriporia reticulata and Pseudocercospora cucurbita) on cucumbers, Pseudocercospora cinerea (Pseudocerosa) on grapes, Pseudoceria graminis (Pseudocerosa and Pseudocerosa (Pseudocerosa) on grapes, Pseudocerosa (Pseudocercospora cinerea), Podocarpa, Pseudocerosa (Pseudocerosa) on grapes, Popularia and Pseudocerosa (Pseudocerosa) on grapes, Popularia farinacea, Popularia farinosa, Popularia and Pseudoperonospora cinerea) on grapes, Podospora cinerea (Pseudoperonospora far, Alternaria on vegetables and fruits (Alternaria), Mycosphaerella on bananas (Mycosphaerella), and Fusarium (Fusarium) and Verticillium (Verticillium).
The bactericidal composition is suitable for plants mainly comprising the following components:
field crops, such as corn, soybean, cotton, canola oil seeds, such as southern canola (Brassica napus) (e.g., canola), turnip (Brassica), mustard (b.juncea) (e.g., mustard (mustard)), and eruca sativa (Brassica carinata), rice, wheat, sugar beet, sugar cane, oats, brown wheat, barley, millet, triticale, flax;
grape vines and fruit or vegetable crops of various plant classes, such as rosaceous fruits (e.g. apples and pears), but also stone fruits, such as apricots, cherries, almonds and peaches, berries such as strawberries), the theaceae (ribeoidae sp), the Juglandaceae (juglaceae sp), the betulinaceae (Betulaceae sp), the Anacardiaceae (Anacardiaceae sp), the Fagaceae (Fagaceae sp), the Moraceae (Moraceae sp), the rhinoceraceae (oleaceae sp), the actinidiaceae (actinoidaceae sp), the Lauraceae (Lauraceae sp), the Musaceae (Musaceae sp), such as trees and plantains (theaceae), the Rubiaceae (theaceae), such as chaetaceae (rosaceae), the Rubiaceae (rosaceae), and the chaetaceae (rosaceae), such as chaetaceae (rosaceae); solanaceae (solanaceae sp.) (e.g. tomatoes, potatoes, peppers, eggplants), Liliaceae (Liliaceae sp.), compositaceae (Compositiae sp.) (e.g. lettuce, artichoke and chicory-including root chicory (root chicory), endive (endive) or common chicory), Umbelliferae (Umbelliferae sp.) (e.g. carrots, parsley, celery and celeries), Cucurbitaceae (Cucurbitaceae sp.) (e.g. cucumbers-including pickled cucumbers (pickling cuumber), squash, watermelons, cucurbits and melons), Alliaceae (Alliaceae sp.) (e.g. onions and leeks), cruciferae.g. brassicae.g. white cabbage, red cabbage, broccoli, cabbage, cauliflower, brussels sprouts, cabbage, parsley, radish, leguminous, lentils such as lentils, beans, chenopodiaceae (Chenopodiaceae sp.) (e.g., fodder beet, spinach sweet (spinach beet), spinach, beetroot), Malvaceae (e.g., okra), asparagines (e.g., asparagus);
horticultural and forest crops;
an ornamental plant; and genetically modified homologues of these crops.
The fungicidal compositions of the invention are particularly suitable for controlling fungi and bacteria on cereals, vegetables, fruits, ornamentals and vines.
The bactericidal composition is particularly suitable for preventing and treating root rot, downy mildew, penicilliosis, green mildew, gray mold, sclerotinia, brown rot, leaf spot, banded sclerotial blight, take-all, rust, anthracnose, early blight, powdery mildew, scab, black spot, gummy stem blight, stalk blight, ring rot, acid rot, crown rot, soft rot, mucor, black spot, black rot, epidemic disease and sclerotinia on cereals, vegetables, fruits, ornamental plants and grapevines.
The fungicidal compositions of the present invention may also be used to prevent or control a variety of pathogenic or saprophytic fungi and bacteria in soils or cultivation media.
Examples of soil-borne fungal pathogens include Alternaria spp, Chaetomium spp, Botrytis cinerea, Cercospora spp, Claviceps purpurea, Cochliospora graminis (Cochliobolus sativus), Colletotrichum spp, Epicoccum spp, Fusarium graminearum (Fusarium graminearum), Fusarium oryzae (Fusarium moniliforme), Fusarium oxysporum (Fusarium oxysporum), Fusarium moniliforme (Fusarium moniliforme), Fusarium solani (Fusarium moniliforme), Fusarium venenatum (Fusarium moniliforme), Fusarium moniliforme (Fusarium septorium), Fusarium solani (Fusarium moniliforme), Fusarium oxysporum (Fusarium moniliforme), Fusarium solani (Fusarium moniliforme), Penicillium solanum (Rhizoctonium), Penicillium solanum (Rhizoctonia), Rhizoctonia solani (Rhizoctonia), Rhizoctoniensis), Rhizoctonia solani (Rhizoctonia solani), Rhizoctonia solani (Rhizoctoniensis), Rhizoctonia solani (Rhizoctonia solani), Rhizoctonia solani (Rhizoctonia), Rhizoctonia solani (Rhizoctoniensis), Rhizoctonia solani (Rhizoctonia solani), utilis species (Urosysticcculta), Ustilago spp or Verticillium spp.
The soil germs include rhizoctonia solani, fusarium, phytophthora, damping-off, root rot, pythium, botrytis cinerea, soft rot and the like. Under general conditions, soil pathogenic bacteria can generate a large amount of bacteria, as long as conditions are favorable for growth and development of the pathogenic bacteria and hosts are susceptible, the pathogenic bacteria can propagate in a large amount and infect the hosts, under the host infected with diseases, the pathogenic bacteria can enter a continuous pathogenic period, propagate and diffuse in a large amount along with continuous cropping of crops, but then nutrients are consumed, or when soil conditions such as temperature, humidity and the like are unfavorable for the pathogenic bacteria, the pathogenic bacteria can enter a dormant period. When the host with disease does not exist, soil-borne disease bacteria can survive in soil, and the soil-borne disease bacteria can survive on the root surface or the fallen leaves of the non-host except the soil-borne disease bacteria with wide host range and have the saprophytic competitive ability. However, different germs are different, and like fusarium can almost survive in soil indefinitely.
The bactericidal mixture can also be used for preventing and controlling diseases in the storage period, and obtains unexpected synergistic effect.
According to the invention, post-harvest and storage-period diseases can be caused, for example, by the following pathogenic bacteria:
colletotrichum species, such as banana Colletotrichum (Colletotrichum musae), Colletotrichum disclinae (Colletotrichum gloeosporioides), capsicum Colletotrichum (Colletotrichum coccodes);
fusarium species, such as Fusarium semitectum (Fusarium semitectum), Fusarium moniliforme (Fusarium moniliforme), Fusarium solani (Fusarium solani), Fusarium oxysporum (Fusarium oxysporum);
verticillium species, such as, for example, Verticillium theobromae (Verticillium theobromae);
a species of the genus Neurospora; botrytis species, such as Botrytis cinerea;
geotrichum species, such as Geotrichum candidum (Geotrichum candidum);
phomopsis species, Phomopsis natalensis (Phomopsis natalensis);
species of the genus Lasiosphaera, such as, for example, Dichloropsis citrifolia (Diplodia citri);
alternaria species, such as, for example, Alternaria citri (Alternaria citri), Alternaria alternata (Alternaria alternata);
phytophthora species, such as Phytophthora citri (Phytophthora citri), Phytophthora fragi (Phytophthora fragaria), Phytophthora infestans (Phytophthora cactorum), Phytophthora nicotiana (Phytophthora parasitica);
septoria (Septoria spp.), for example, Septoria depressa;
mucor spp, such as Mucor piriformis (Mucor piriformis);
streptomyces (Monilinia spp.), such as, for example, Streptomyces fructicola (Monilinia fructicola), Streptomyces drupes (Monilinia laxa);
venturia spp, such as Venturia inaequalis, Venturia pyrifera (Venturia inaegulis), Venturia pyrifolia (Venturia pyrina);
rhizopus sp, such as Rhizopus stolonifer, Rhizopus oryzae (Rhizopus oryzae);
genus Microtheca (Glomerella spp.), e.g., Percocephala (Glomerella cingulata); sclerotinia spp, such as Sclerotinia fructicola (Sclerotinia fructicola);
the genus longbeak (Ceratocystis spp.), such as the Kiwi long beak (Ceratocystis paradoxa);
penicillium spp, such as Penicillium funiculosum (Penicillium funiculosum), Penicillium expansum (Penicillium expandasum), Penicillium digitatum (Penicillium digitatum), Penicillium italicum (Penicillium italicum);
pediophora sp, e.g., Pediophora albuginea (Gloeosporium album), Gloeosporium perennans, Pediophora fructicola (Gloeosporium fructigenum), Gloeosporium singulata;
humularia (Phlyctaenana spp.) such as Phlyctaena vagabunda;
cylindrocarpon spp, such as, for example, Cylindrocarpon mali; stemphylium spp, such as stemphylium citrinum (stemphylium venelicanum);
aschersonia (Phacydiopanis spp.), e.g., Phacydiopani malirum; rhizopus spp, such as Rhizopus mirabilis paradoxy;
aspergillus spp, such as Aspergillus niger, Aspergillus carbonarius;
genus Nectria (Nectria spp.), such as, for example, Lectria drynaria (Nectria galligena);
amycolatopsis (Pezicula spp.).
The bactericidal composition is also particularly suitable for preventing and treating acid rot, penicilliosis, green mold, black rot, stalk rot, scorch, anthracnose, crown rot, frost blight, gray mold, brown rot, soft rot, hair mold, brown scald, bitter pox, black heart disease and soft rot of fruits and vegetables during storage.
The bactericidal composition is used for preventing and treating diseases of fruits or vegetables in the storage period.
The use of the fungicidal compositions of the present invention for protecting fruit or vegetable stock from fungal or bacterial infestation during the shelf life.
The bactericidal composition is particularly suitable for preventing and treating diseases of citrus in a storage period.
The bactericidal composition is particularly suitable for preventing and treating acid rot, penicilliosis, green mold and scorch of oranges in the storage period.
The invention also provides a method for controlling phytopathogenic fungi by applying the fungicidal composition to the pathogenic fungi and/or their environment, or to plants, plant propagation material and to the organs, soils, materials or spaces, stores of plants which grow subsequently.
A method for controlling phytopathogenic fungi comprises simultaneously or separately applying propiconazole and pyrimethanil to the pathogenic fungi and/or their environment, or to the plants, to plant propagation material and to the plant organs, soil, materials or spaces, stores which grow out later.
The invention also provides a method for controlling phytopathogenic fungi by applying the fungicidal compositions according to the invention to the pathogenic fungi and/or their environment, or to plants, plant propagation material and to plant organs, soils, materials or spaces, stores which grow at a later time, by seed treatment, foliar application, stem application, drenching, instillation, pouring, spraying, dusting, scattering or fuming.
The bactericidal composition can be used as a foliar fungicide in crop protection, can also be used as a fungicide for seed dressing and as a soil fungicide, and can also be used as a preservative for postharvest storage.
The bactericidal composition of the present invention can treat all plants. "plant" means all plants and plant populations such as desirable and undesirable wild plants, cultivars, and plant varieties (whether or not protected by a plant variety or plant cultivar rights-to-human). Cultivated plants and plant varieties may be plants obtained by conventional propagation and cultivation methods, which may be supplemented or supplemented by one or more biotechnological methods, for example using dihaploids, protoplast fusion, random and directed mutations, molecular or genetic markers, or using bioengineering and genetic engineering methods. Plant parts are understood to mean all parts and organs of plants above and below the ground, such as shoots, leaves, flowers and roots, such as leaves, needles, stems, branches, flowers, fruit bodies, fruits and seeds, and also roots, bulbs and rhizomes. Also plants and vegetative and generative propagation material, for example cuttings, bulbs, rhizomes, runners and seeds, belong to the plant part.
The term "plant propagation material" is understood to mean all reproductively competent plant parts, such as seeds, which can be used for the propagation of the latter, and also vegetative materials, such as cuttings or tubers (e.g. potatoes). Thus, plant parts as used herein include plant propagation material. Mention may be made, for example, of seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated plants and useful plants to be inhibited after germination or after emergence from the soil. The young plants can be protected prior to transplantation by a total or partial treatment by dipping.
Preferred plant propagation material of the present invention is a seed. The fungicidal compositions of the present invention are also particularly suitable for the treatment of seeds. Most of the crop damage caused by harmful fungi is caused by the attack of the seeds during storage or after sowing and during or after germination of the plants. Plants are particularly sensitive to roots and shoots during the growing period and can cause death of the plant even if there is little damage. This stage is particularly critical, since it is very important to protect the seeds and the germinating plants by using suitable compositions.
Another aspect of the present invention provides a method for protecting seeds and germinating plants, which method makes it unnecessary or at least significant additional application of crop protection agents after sowing or after the emergence of the plants. On the other hand, the amount of active compound used is optimized with the fungicidal composition according to the invention in order to provide maximum protection of the seeds and the germinating plants from attack by phytopathogenic fungi, without the plants themselves being harmed by the active compound used.
The present invention therefore also relates in particular to a method for protecting seeds and germinating plants from attack by phytopathogenic fungi by treating the seeds with a fungicidal composition according to the present invention. The invention also relates to the use of the composition according to the invention for treating seeds to protect the seeds and germinating plants from phytopathogenic fungi.
The fungicidal compositions according to the invention are suitable for protecting the seed of any plant variety applied in agriculture, in greenhouses, in forestry or in horticulture-or grape cultivars. In particular, it takes the form of seeds of cereals (such as wheat, barley, rye, triticale, millet, oats), maize, cotton, soybeans, rice, potatoes, sunflowers, beans, coffee, sugar beet, peanuts, oilseed rape, olives, cocoa, sugar cane, tobacco, vegetables (such as tomatoes, cucumbers, onions and lettuce), turfgrass and decorative plants. The treatment of cereal and vegetable seeds is of vital importance.
The active ingredients of the fungicidal composition of the present invention, propiconazole and pyrimethanil, are applied to seeds either alone or in a suitable formulation. It is preferably treated in a sufficiently stable state that the treatment does not cause any damage to the seed. In general, the treatment of the seeds can be carried out at any point in time between picking and sowing. The seeds commonly used are isolated from the plant and from the cob, husk, stem, cuticle, hair or pulp. Thus, for example, seeds that have been picked, cleaned and dried to a moisture content of less than 15% may be used. Alternatively, seeds may be used which are dried, for example by treatment with water, and then dried again.
Examples of the method of seed treatment include a method of diluting a liquid or solid chemical, a method of directly immersing seeds in a liquid solution without dilution to allow the chemical to permeate the seeds, a method of mixing a solid chemical or liquid chemical with seeds to coat the seeds and thereby adhering the chemical to the surfaces of the seeds, and a method of spraying the chemical to the vicinity of the seeds while planting.
The control of phytopathogenic fungi which damage the post-emergent plants is effected primarily by treating the soil and the aerial parts of the plants with crop protection agents.
The fungicidal compositions of the present invention may also be used to prevent or control a variety of pathogenic or saprophytic fungi and bacteria in soils or cultivation media. Examples of methods for applying a chemical to soil include a method in which a liquid chemical is diluted in water or applied without dilution directly to the roots of a plant or a seedling bed for raising seedlings, a method in which granules are sown to the roots of a plant or a seedling bed for raising seedlings by spraying a powder, a water dispersible granule or the like to soil and mixing with the whole soil before sowing, and a method in which a powder, a water dispersible granule or the like is diluted and sprayed to a planting hole or a planting furrow before sowing or planting a plant, and sowing is performed.
The fungicidal compositions of the present invention may also be used to protect stored products from fungal and bacterial infestation.
According to the invention, the term "stock" is understood to mean natural substances and processed forms thereof of plant or animal origin which have been derived from the natural life cycle and are intended to be preserved for a long period of time. Storage products of plant origin, for example plants or parts thereof, such as stems, leaves, tubers, seeds, fruits or grains, can be protected in the freshly harvested state or in processed form, such as (pre) drying, wetting, comminuting, grinding, pressing or baking. Or wood, in the form of coarse wood such as construction timber, utility poles and fences; or in finished form, such as furniture or articles made of wood. The animal-derived stock is hide, leather, hair, etc. The compositions according to the invention can prevent fungal or bacterial attack such as corrosion, discoloration or mildew on storage. "stock" is preferably understood to mean natural substances of plant origin and processed forms thereof, more preferably fruits or vegetables, processed forms thereof.
The present invention also provides a method of protecting a fruit or vegetable stock from fungal or bacterial infestation during storage comprising contacting the stock with a germicidal composition of the invention.
The present invention provides a method of protecting fruit or vegetable stock from fungal or bacterial infestation during storage comprising: (i) before harvesting; (ii) after harvesting; or (i) and (ii), contacting the germicidal composition of the present invention with the fruit or vegetable.
Therefore, the bactericidal composition can be used as a foliar fungicide in crop protection, as a fungicide for seed dressing and as a soil fungicide, and as a preservative for postharvest storage.
The germicidal compositions of the present invention may be applied by various treatment methods, such as:
-spraying a liquid comprising the fungicidal composition onto the above-ground parts of the plant;
-dusting, incorporating granules or powders in the soil, spraying around said plants and, in the case of tree injection or painting;
-coating or film coating the seeds of the plants.
When the preservative is used for preserving and preserving fruits or vegetables after picking, the preservative is usually diluted by water by 200 and 2000 times, and the fruits are drained after soaking.
The present invention provides a method for controlling plant pathogenic bacteria, which can be a treatment, prevention or eradication method.
The fungicidal composition of the present invention can be used in the form of a conventional chemical preparation, for example, emulsifiable concentrate, wettable powder, suspension, liquid, granule, seed coating, and the like, and the application amount thereof varies depending on the mixing ratio of the active ingredients, weather conditions, the chemical preparation, the application time, the application method, the application site, the control of target pests, the target crop, and the like.
Typically for leaf treatment: 0.1 to 10000g/ha, preferably 10 to 1000g/ha, more preferably 50 to 500 g/ha; for dipping or instillation administration, the dosage may even be reduced, particularly when an inert substrate such as asbestos or perlite is applied;
-for seed treatment: 2-5000g/100kg of seeds, preferably 3-1000g/100kg of seeds;
-applying a treatment to the soil or water surface: 0.1 to 10000g/ha, preferably 1 to 1000 g/ha.
For the preservation of picked fruits and vegetables, 200 times and 2000 times of liquid can be diluted, and the fruits can be drained after being soaked.
The above-mentioned dosages are only typical exemplary dosages, and the person skilled in the art will adjust the application rate in the actual application according to the actual circumstances and needs, in particular according to the nature of the plants or crops to be treated and the severity of the germs.
Propiconazole of the invention is administered in combination/association with pyrimethanil. Comprising separate, sequential or simultaneous administration of propiconazole and pyrimethanil. Preferably, the combination of propiconazole and pyrimethanil is in the form of a composition comprising propiconazole and pyrimethanil.
The composition of the present invention may be in the form of a preparation, i.e., the substances in the composition have been mixed; the ingredients of the composition may also be provided in a single dose, mixed in a tub or tank prior to use, and then diluted to the desired concentration. The preparation form provided by the invention is preferably the main form.
The sterilization composition has the advantages that:
the invention provides a bactericidal composition, which is prepared by binary compounding of propiconazole and pyrimethanil, so that the obtained composition has a gain effect on the prevention and treatment effects, the bactericidal spectrum is expanded, the effect of one medicine for multiple purposes is achieved, and the generation of drug resistance of germs is effectively slowed down or avoided. Surprisingly, the fungicidal activity of the fungicidal compositions according to the invention is significantly higher than the sum of the activities of the individual active compounds. In other words, there is an unpredictable, truly present synergistic effect, not just a supplementation of activity.
A composite bactericide for preventing and eliminating the fungal and bacterial diseases of vegetables, fruit tree and cereal is prepared from propiconazole and pyrimethanil. The composition containing propiconazole and pyrimethanil is very effective not only against common plant pathogens but also against pathogens which have developed drug resistance, and has excellent control effects even under conditions where diseases continue to develop, thus completing the present invention.
Detailed Description
The invention will be further illustrated with reference to the following examples:
formulation examples:
example 150% pyrimethanil +10% propiconazole wettable powder
50 percent of pyrimethanil
Propiconazole 10%
Sodium dodecyl sulfate 10%
Sodium lignosulfonate 5%
White carbon black 10%
Kaolin is complemented to 100%
Mixing the active compound, various auxiliaries, fillers and the like according to the proportion of the formula, and crushing by using an ultrafine crusher to obtain the 50% pyrimethanil +10% propiconazole wettable powder.
Example 240% pyrimethanil +10% propiconazole wettable powder
Pyrimethanil 40%
Propiconazole 10%
1 percent of calcium dodecyl benzene sulfonate
Sodium lignosulfonate 2%
Sucrose to make up to 100%
Mixing the active compound, various auxiliary agents, fillers and the like according to the proportion of the formula, and crushing the mixture by using an ultrafine crusher to obtain the wettable powder of 40 percent pyrimethanil and 10 percent propiconazole.
Example 320% pyrimethanil +10% propiconazole emulsifiable concentrate
20 percent of pyrimethanil
Propiconazole 10%
Ethoxylated castor oil 5%
Calcium dodecyl benzene sulfonate 3%
Ethyl acetate to make up to 100%
The components are prepared according to the proportion and are stirred uniformly to obtain a uniform phase.
Example 45% pyrimethanil +1% propiconazole ultra Low volume liquid formulation
Pyrimethanil 5%
1 percent of propiconazole
2 percent of polyoxyethylene alkyl aryl ether,
Ethyl acetate to make up to 100%
Example 530% pyrimethanil +10% propiconazole Water dispersible granules
Pyrimethanil 30%
Propiconazole 10%
5 percent of modified calcium lignosulfonate
Sodium dodecyl sulfate 5%
5 percent of urea
Kaolin is complemented to 100%
Mixing pyrimethanil, propiconazole, a dispersing agent, a wetting agent, a disintegrating agent and a filler uniformly according to the proportion of a formula, and performing air flow crushing to obtain wettable powder; adding a certain amount of water, mixing and extruding to obtain the finished product. Drying and screening to obtain the 30% pyrimethanil and 10% propiconazole water dispersible granule.
Example 650% pyrimethanil +10% propiconazole Suspoemulsion
Oil phase:
50 percent of pyrimethanil
Oleic acid methyl ester 10%
Ethoxylated castor oil 5%
Water phase:
propiconazole 20%
Sodium salt of sulfonated naphthalene sulfonic acid-formaldehyde condensation product 1%
The water is complemented to 100 percent
Dissolving propiconazole in methyl oleate, and adding ethoxylated castor oil to obtain an oil phase; grinding and/or shearing pyrimethanil, sodium salt of a sulfonated naphthalene sulfonic acid-formaldehyde condensation product and water at a high speed according to a formula to obtain a pyrimethanil suspending agent; the oil phase was added to the aqueous phase under stirring to obtain a suspoemulsion.
Example 730% pyrimethanil +5% propiconazole emulsion in water
Pyrimethanil 30%
Propiconazole 5%
30 percent of ethyl acetate
Polyoxyethylene polyoxypropylene Block Polymer 8%
Ethylene glycol 5%
The water is complemented to 100 percent
The pyrimethanil, the propiconazole, the solvent, the emulsifier and the like are added together to be dissolved into a uniform oil phase; water, antifreeze, defoamer, etc. are mixed together to form a uniform water phase. Adding the water phase into the oil phase under high-speed stirring to obtain the aqueous emulsion.
Example 810% pyrimethanil +5% propiconazole coated granules
Pyrimethanil 10%
Propiconazole 5%
3 percent of polyethylene glycol
1% of highly dispersed silicic acid
Calcium carbonate to make up to 100%
The finely ground fungicidal active compound is homogeneously spread on the carrier moistened with polyethylene glycol in a mixer. In this way dust-free coated granules are obtained.
EXAMPLE 950% pyrimethanil +10% propiconazole wettable powder
50 percent of pyrimethanil
Propiconazole 10%
Sodium dodecyl sulfate 1%
1 percent of sodium lignosulfonate
Kaolin is complemented to 100%
The components are mixed according to a proportion, ground and crushed to prepare the wettable powder.
Example 1020% Pyrimidinel +5% propiconazole granules
20 percent of pyrimethanil
Propiconazole 5%
Sodium lignosulfonate 4%
2 percent of carboxymethyl cellulose
Kaolin is complemented to 100%
The active compounds are mixed with auxiliaries and milled, moistened with water, granulated and then dried in an air stream.
Example 1120% pyrimethanil +10% propiconazole coating
20 percent of pyrimethanil
Propiconazole 10%
5 percent of disodium fatty alcohol polyoxyethylene ether sulfosuccinate monoester
5 percent of modified calcium lignosulfonate
Xanthan gum 1%
1 percent of bentonite
Glycerol 5%
PVP-K30 1%
The water is complemented to 100 percent
The seed coating agent is obtained by mixing the components in proportion and grinding and/or high-speed shearing.
Example 1230% pyrimethanil +10% propiconazole microcapsule suspension-suspension
Capsule core:
propiconazole 10%
Plurafac LF1312(from BASF) 3%
Emulsogen 3510 (from Clariant) 1%
Capsule wall:
Lupranat®M20S(BASF Elastogran) 8%
DETA (BASF SE) 2%
water phase:
pyrimethanil 30%
ATLOX ®4913 (from Croda) 5%
Synperonic PE/64 3%
Xanthan gum 1%
1 percent of defoaming agent
1.0 percent of urea
1, 2-propylene glycol 3%
2 percent of methylisothiazolinone
0.1 percent of catalyst
The water is complemented to 100 percent
Oil phases formed by Lupranat M20S, propiconazole, Plurafac LF1312 and Emulsogen 3510 are added into aqueous solution containing DETA and ATLOX 4913 to form emulsion. Then heating and maintaining the temperature at 50 DEGoAdding catalyst to react for 2 h. Cooling to obtain the microcapsule of propiconazole.
Synperonic PE/64(from Croda), a defoaming agent, urea, pyrimethanil and water are mixed uniformly according to a certain proportion and are sanded to prepare the aqueous suspending agent.
The microcapsule containing propiconazole is added into the pyrimethanil suspending agent to obtain the microcapsule suspension-suspending agent.
Example 1325% Pyrimidil +5% propiconazole Suspoemulsion
Pyrimethanil 25%
Propiconazole 5%
SOLVESSOTM200 5%
Ethoxylated castor oil 4%
5 percent of disodium fatty alcohol polyoxyethylene ether sulfosuccinate monoester
5 percent of modified calcium lignosulfonate
Xanthan gum 1%
1 percent of bentonite
Glycerol 5%
The water is complemented to 100 percent
Dissolving propiconazole in SOLVESSOTM200, adding ethoxylated castor oil to obtain an oil phase of the propiconazole;
mixing the fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoester disodium, the modified calcium lignosulfonate, the pyrimethanil and water in proportion, and grinding and/or shearing at high speed to obtain the pyrimethanil suspending agent.
Adding the oil phase containing propiconazole into the water suspending agent containing pyrimethanil to obtain the suspending emulsion.
Example 1430% pyrimethanil +10% propiconazole Water dispersible granules
Pyrimethanil 30%
Propiconazole 10%
5 percent of modified calcium lignosulfonate
Sodium dodecyl sulfate 5%
5 percent of urea
Kaolin is complemented to 100%
Mixing pyrimethanil, propiconazole, a dispersing agent, a wetting agent, a disintegrating agent and a filler uniformly according to the proportion of a formula, and performing air flow crushing to obtain wettable powder; adding a certain amount of water, mixing and extruding to obtain the finished product. Drying and screening to obtain the 30% pyrimethanil and 10% propiconazole water dispersible granule.
Example 1520% pyrimethanil +20% propiconazole Water dispersible granules
20 percent of pyrimethanil
Propiconazole 20%
5 percent of modified calcium lignosulfonate
Sodium dodecyl sulfate 5%
5 percent of urea
Kaolin is complemented to 100%
Mixing pyrimethanil, propiconazole, a dispersing agent, a wetting agent, a disintegrating agent and a filler uniformly according to the proportion of a formula, and performing air flow crushing to obtain wettable powder; adding a certain amount of water, mixing and extruding to obtain the finished product. And drying and screening to obtain the 20% pyrimethanil and 20% propiconazole water dispersible granule.
Example 1640% pyrimethanil +5% propiconazole wettable powder
Pyrimethanil 40%
Propiconazole 5%
Sodium dodecyl sulfate 1%
1 percent of sodium lignosulfonate
Kaolin is complemented to 100%
The components are mixed according to a proportion, ground and crushed to prepare the wettable powder.
Example 1770% pyrimethanil +30% propiconazole
70 percent of pyrimethanil
Propiconazole 30%
Mixing the above materials at a certain proportion, and making into powder.
The proportion in the above examples is weight percent.
Biological test example
Indoor toxicity assay
Test one: virulence determination of sigatoka
Adopting a method for inhibiting the growth rate of hypha: the test target is the sigatoka bacteria.
Respectively dissolving propiconazole and pyrimethanil with acetone, diluting with 0.1% tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL of the liquid medicines into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with the diameter of 9cm, and preparing into 4 toxic culture media with corresponding concentrations; the toxicity-containing culture medium is prepared by compounding liquid medicines of propiconazole and pyrimethanil with different ratios in the same method. The banana leaf spot pathogen cultured for 2 days is beaten into bacterial blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are placed in an incubator at 25 ℃ for culture, and the treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
then calculating the concentration EC in the inhibition by using a least square method50Then, the cotoxicity coefficient (CTC) was calculated by the Sun Yunpei method.
Table 1: virulence test results for sigatoka
As can be seen from Table 1, when the combination of propiconazole and pyrimethanil is used for preventing and treating sigatoka in the range of the mixture ratio of 10:1-1:50, the co-toxicity coefficients are all larger than 120, and the synergistic effect is shown.
And (2) test II: toxicity assay for penicilliosis in citrus
Adopting a method for inhibiting the growth rate of hypha: the test target is citrus penicilliosis.
Respectively dissolving propiconazole and pyrimethanil with acetone, diluting with 0.1% tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL of the liquid medicines into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with the diameter of 9cm, and preparing into 4 toxic culture media with corresponding concentrations; the toxicity-containing culture medium is prepared by compounding liquid medicines of propiconazole and pyrimethanil with different ratios in the same method. The penicillium citrinum cultured for 2 days is beaten into bacterium blocks at the edges of the bacterial colonies by a puncher with the diameter of 5mm, the bacterium blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterium blocks are cultured in an incubator at 25 ℃, and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
then calculating the concentration EC in the inhibition by using a least square method50Calculating co-toxicity coefficient (CTC) by Sun Yunpei method。
Table 2: toxicity test results for citrus penicilliosis
As can be seen from Table 2, when the combination of propiconazole and pyrimethanil is used for preventing and treating penicilliosis in citrus, and the propiconazole and the pyrimethanil have the co-toxicity coefficient of more than 120 in the range of the ratio of 10:1-1:50, the synergistic effect is shown.
And (3) test III: toxicity determination for citrus anthracnose
Spore germination test method is adopted. Selecting citrus seedlings with consistent growth vigor, selecting 3 pots of citrus seedlings to be tested for each treatment, spraying by using a potter spray tower under the pressure of 50PSI, wherein each pot is about 5mL, and each medicament is provided with 12 concentration gradients. Inoculating bacteria 24h after the treatment of the medicament, uniformly shaking off conidia above citrus seedlings to inoculate diseased leaves infected with citrus anthracnose, and then putting the citrus seedlings into a greenhouse for culturing. 7d, investigating disease index according to disease classification standard, calculating prevention and treatment effect, and calculating inhibition medium concentration EC by least square method50Then, the cotoxicity coefficient (CTC) was calculated by the Sun Yunpei method.
The calculation method comprises the following steps:
actually measured virulence index (ATI) = (standard agent EC)50Reagent for test EC50）*100
Theoretical virulence index (TTI) = a agent virulence index% percentage of a in a mixture + B agent virulence index% percentage of B in a mixture
Co-toxicity coefficient (CTC) = [ actually measured toxicity index (ATI)/Theoretical Toxicity Index (TTI) × 100 for mixed agent)
CTC <80 is antagonistic action, CTC <80 > 120 is additive action, CTC > 120 is synergistic action,
table 3: toxicity test result on citrus anthracnose
As can be seen from Table 3, when the combination of propiconazole and pyrimethanil is used for preventing and treating citrus anthracnose, and the weight ratio of the propiconazole to the pyrimethanil is 10:1-1:50, the co-toxicity coefficients are both greater than 120, and a synergistic effect is shown.
And (4) testing: determination of toxicity to bacterial soft rot of cabbage
Spore germination test method is adopted. Selecting cabbage plants with consistent growth vigor, selecting 3 pots of tested cabbage plants for each treatment, spraying by using a potter spray tower under the pressure of 50PSI, wherein each pot is about 5mL, and each medicament is provided with 12 concentration gradients. Inoculating bacteria 24h after the medicament treatment, uniformly shaking off conidia above Chinese cabbage plants to inoculate diseased leaves infected with Chinese cabbage bacterial soft rot, and then putting the Chinese cabbage plants into a greenhouse for culturing. 7d, investigating disease index according to disease classification standard, calculating prevention and treatment effect, and calculating inhibition medium concentration EC by least square method50Then, the cotoxicity coefficient (CTC) was calculated by the Sun Yunpei method.
The calculation method comprises the following steps:
actually measured virulence index (ATI) = (standard agent EC)50Reagent for test EC50）*100
Theoretical virulence index (TTI) = a agent virulence index% percentage of a in a mixture + B agent virulence index% percentage of B in a mixture
Co-toxicity coefficient (CTC) = [ actually measured toxicity index (ATI)/Theoretical Toxicity Index (TTI) × 100 for mixed agent)
CTC <80 is antagonistic action, CTC <80 > 120 is additive action, CTC > 120 is synergistic action,
table 4: toxicity test result on cabbage bacterial soft rot
As can be seen from Table 4, the combination of propiconazole and pyrimethanil can prevent and treat Chinese cabbage bacterial soft rot, and the co-toxicity coefficients of the propiconazole and the pyrimethanil are both greater than 120 when the weight ratio of the propiconazole to the pyrimethanil is in the range of 10:1-1:50, so that the synergistic effect is shown.
And (5) testing: virulence determination of citrus acid rot
Adopting a method for inhibiting the growth rate of hypha: the test target is citrus acid rot.
Respectively dissolving propiconazole and pyrimethanil with acetone, diluting with 0.1% tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL of the liquid medicines into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with the diameter of 9cm, and preparing into 4 toxic culture media with corresponding concentrations; the toxicity-containing culture medium is prepared by compounding liquid medicines of propiconazole and pyrimethanil with different ratios in the same method. The citrus acidovorax citrulli cultured for 2 days is beaten into bacterial blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are placed in an incubator at 25 ℃ for culture, and the treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
then calculating the concentration EC in the inhibition by using a least square method50Then, the cotoxicity coefficient (CTC) was calculated by the Sun Yunpei method.
Table 5: toxicity test result for citrus acid rot
As can be seen from Table 5, the combination of propiconazole and pyrimethanil can prevent and treat citrus acid rot, and when the proportions of propiconazole and pyrimethanil are in the range of 10:1-1:50, the co-toxicity coefficients are both greater than 120, so that the synergistic effect is shown.
Claims (18)
1. The bactericidal composition is characterized in that active ingredients comprise propiconazole and pyrimethanil, and the weight ratio of the propiconazole to the pyrimethanil is 10:1-1: 50.
2. The bactericidal composition of claim 1, wherein the weight ratio of propiconazole to pyrimethanil is from 5:1 to 1: 25.
3. The bactericidal composition of claim 1, wherein the weight ratio of propiconazole to pyrimethanil is from 5:1 to 1: 20.
4. The bactericidal composition of claim 1, wherein the weight ratio of propiconazole to pyrimethanil is from 1:1 to 1: 15.
5. The bactericidal composition of claim 1, wherein the weight ratio of propiconazole to pyrimethanil is from 1:5 to 1: 15.
6. The germicidal composition of claim 1, further comprising a filler and/or a surfactant.
7. The bactericidal composition of claim 1, wherein the dosage form is wettable powder, emulsifiable concentrate, suspending agent, suspoemulsion, microcapsule, seed coating agent, microemulsion, aqueous emulsion, water dispersible granule, foaming agent, paste, aerosol, ultra-low volume liquid, ultra-low volume powder, granule, or effervescent tablet.
8. The germicidal composition of claim 1, wherein the active ingredients propiconazole and pyrimethanil comprise 1% to 90% of the germicidal composition.
9. The germicidal composition of claim 1, wherein the active ingredients propiconazole and pyrimethanil comprise 5% to 80% of the germicidal composition.
10. The germicidal composition as claimed in claim 1, wherein the active ingredients of propiconazole and pyrimethanil account for 10-60% of the germicidal composition.
11. Use of the fungicidal composition according to claim 1 for controlling fungi and bacteria on cereals, vegetables, fruits, ornamentals and grapevines, selected from the group consisting of myceliophthora cucumerinum, erysiphe necator, peronospora cubensis, botrytis cinerea, pyrenophora hordeum and phytophthora infestans.
12. Use of the fungicidal composition according to claim 1 for controlling shelf-life diseases of fruits or vegetables involving fungi selected from the group consisting of Rhizoctonia solani, Stachybotrys graminis, Pseudoperonospora cubensis, Botrytis cinerea, Pyrenophora hordeum, and Phytophthora infestans.
13. Use of the fungicidal composition of claim 1 for protecting fruit or vegetable stocks from infestation by fungi or bacteria during storage, the fungi being selected from the group consisting of myceliophthora cucumericola, erysiphe viticola, peronospora cubensis, botrytis cinerea, pyrenophora horrida and phytophthora infestans.
14. A method of controlling phytopathogenic fungi selected from the group consisting of myceliophthora cucumerinum, erysiphe necator, peronospora cubensis, botrytis cinerea, sclerotinia barley and phytophthora infestans, comprising applying the fungicidal composition of claim 1 to the pathogenic fungi and/or their environment, or to the plant, seed, soil, material or space, storage.
15. A method for controlling phytopathogenic fungi selected from the group consisting of myceliophthora cucumeris, erysiphe viticola, peronospora cubensis, botrytis cinerea, sclerotinia graminearum and phytophthora infestans of barley, by simultaneous or separate application of propiconazole and pyrimethanil according to claim 1 to the pathogenic bacteria and/or to their environment, or to the plant, seed, soil, material or space, storage.
16. A method for controlling phytopathogenic fungi, characterized in that a fungicidal composition according to claim 1 is applied to the pathogenic fungi selected from the group consisting of chaetomium cupreum, erysiphe necator, peronospora cubensis, botrytis cinerea, sclerotinia barley and phytophthora infestans, and/or to the environment thereof, or to the plants, seeds, soil, materials or spaces, storage, in the form of seed treatments, foliar applications, stem applications, drenchings, instillations, sprays, dusting, scattering or fuming.
17. A method of protecting a fruit or vegetable stock from infestation by fungi or bacteria during storage comprising contacting the stock with the fungicidal composition of claim 1, wherein the fungi are selected from the group consisting of myceliophthora cucumerica, erysiphe necator, peronospora cucumerinum, botrytis cinerea, barley sclerotinia graminea, and phytophthora infestans.
18. A method of protecting a fruit or vegetable stock from fungal or bacterial infestation during storage, comprising: (i) before harvesting; (ii) after harvesting; contacting the fungicidal composition of claim 1 with a fruit or vegetable at (i) and (ii), wherein the fungus is selected from the group consisting of myceliophthora cucumericola, erysiphe necator, peronospora cubensis, botrytis cinerea, barley mycosphaerella graminicola, and phytophthora infestans.
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| WO2004036996A1 (en) * | 2002-10-24 | 2004-05-06 | Bayer Cropscience S.A. | Use of pyrimethanil on resistant pathogens |
| CN102106349A (en) * | 2009-12-23 | 2011-06-29 | 中国中化股份有限公司 | Antifungal composition containing tebuconazole and pyrimidine compound |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2004036996A1 (en) * | 2002-10-24 | 2004-05-06 | Bayer Cropscience S.A. | Use of pyrimethanil on resistant pathogens |
| CN102106349A (en) * | 2009-12-23 | 2011-06-29 | 中国中化股份有限公司 | Antifungal composition containing tebuconazole and pyrimidine compound |
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