CN107637601B - Bactericidal composition - Google Patents
Bactericidal composition Download PDFInfo
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- CN107637601B CN107637601B CN201610542773.1A CN201610542773A CN107637601B CN 107637601 B CN107637601 B CN 107637601B CN 201610542773 A CN201610542773 A CN 201610542773A CN 107637601 B CN107637601 B CN 107637601B
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Abstract
The invention provides a sterilization composition, which comprises active ingredients of fluazinam and hymexazol, wherein the weight percentage of the fluazinam to the hymexazol is 100:1-1: 100. The invention also relates to the use of said fungicidal compositions for protecting plants, plant propagation material and plant organs that grow out later. The invention also relates to the use of the fungicidal compositions for controlling pathogenic or saprophytic fungi and bacteria in soils or cultivation media, by application to the locus where control is desired. The invention also relates to the use of said fungicidal compositions for the treatment of seeds in order to protect against attack by phytopathogenic fungi carried by the seeds.
Description
Technical Field
The present invention relates to a germicidal composition; the invention also relates to a method for controlling harmful fungi by using the sterilization composition. The invention also relates to the use of said fungicidal compositions for the treatment of seeds in order to protect against attack by phytopathogenic fungi carried by the seeds. The invention also relates to the use of the fungicidal compositions for controlling pathogenic or saprophytic fungi and bacteria in soils or cultivation media, by application to the locus where control is desired.
Background
Fungicides are compounds of natural or synthetic origin which are used to protect plants against damage caused by fungi. Current agricultural methods rely heavily on the use of fungicides. In fact, some crops do not grow efficiently without the use of fungicides. The use of fungicides allows the grower to increase the yield and quality of the crop and thereby increase the value of the crop. In most cases, the value of the crop is increased by at least three times the cost of using the fungicide.
However, no single fungicide is useful in all cases, and repeated use of a single fungicide often results in resistance to that or a related fungicide. Thus, research is being conducted to produce fungicides and compositions of fungicides that are safer, have better performance, require lower dosages, are easy to use, and are less costly.
Since the environmental and economic requirements for fungicides are now constantly increasing, for example with regard to the spectrum of activity, toxicity, selectivity, application rate, residue composition and advantageous production feasibility, and also with regard to, for example, resistance, it is a continuing task to develop new fungicides which are superior in some respects to the existing fungicides.
Fluazinam (Fluazinam) is a pyridinamine-based fungicide developed by ICI agrichemicals, developed by the japanese stone industries. The chemical name of fluazinam is 3-chloro-N- (3-chloro-5-trifluoromethyl-2-pyridyl) -alpha, alpha-trifluoro-2, 6-dinitro-p-toluidine. The structural formula is as follows:
hymexazol (hymexazol), chemical name: 5-methyl-1, 2-oxazol-3-ol; the structural formula is
Disclosure of Invention
The object of the present invention is to address the above-mentioned disadvantages by providing a fungicidal composition which provides improved activity against harmful fungi with a reduced total amount of active compound applied (synergistic composition) in order to reduce the application rates of known active compounds and to improve the activity spectrum thereof.
Furthermore, we have found that the simultaneous, i.e. combined or separate, application of fluazinam and hymexazol, or the sequential application of fluazinam and hymexazol, allows better control of harmful fungi than the individual compounds applied alone.
The invention discloses a sterilization composition, which is realized by adopting the following technical scheme:
a germicidal composition, characterized by: the active ingredients consist of fluazinam and hymexazol in a weight percentage of 100:1-1:100, preferably 50:1-1:50, more preferably 40:1-1:40, more preferably 35:1-1:35, more preferably 30:1-1:30, more preferably 25:1-1:25, more preferably 20:1-1:20, more preferably 15:1-1:15, more preferably 10:1-1:10, and further preferably 5:1-1: 5.
The synergistic effect of the fluazinam and the hymexazol is particularly obvious in the range of the mixture ratio.
Fluazinam and hymexazol may exist in different crystal forms.
The weight ratio of fluazinam to hymexazol in the present invention may be, for example, 100:1, 50:1, 45:1, 40:1, 35:1, 30:1, 25:1, 20:1, 19:1,18:1,17:1,16:1, 15:1,14:1,13:1,12:1,11:1, 10:1,9:1,8:1,7:1,6:1, 5:1,4:1,3:1,2:1, 1:1,1:2,1:3,1:4, 1:5,1:6,1:7,1:8,1:9, 1:10,1:11,1:12,1:13,1:14, 1:15,1:16,1:17,1:18,1:19, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50, 100: 1.
The sum of the mass of the fluazinam and the hymexazol in the bactericidal composition accounts for 0.5-90%, more preferably 1-90%, more preferably 5-90%, more preferably 10-80%, and more preferably 20-60% of the mass of the bactericidal composition.
In the bactericidal composition of the present invention, the content of fluazinam and hymexazol may be, for example, 0.5%, 1%, 5%,10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% by weight of the bactericidal composition.
A bactericidal composition can be prepared into any agriculturally allowable dosage form. The formulation of the bactericidal composition is missible oil, aqueous suspension, oil suspension, seed treatment dry powder, seed treatment suspension, water dispersible granules, wettable powder, suspoemulsion, aerosol, coated granules, extruded granules, aqueous emulsion, microcapsule suspension-suspension, dry suspension, ultra-low volume liquid, electrostatic oil and gel.
The bactericidal composition can be applied without dilution or diluted with water.
A method for preventing and treating plant pathogenic bacteria comprises applying bactericidal composition to pathogenic bacteria and/or its environment, or plant, seed, soil, material or space.
A method for preventing and treating plant pathogenic bacteria comprises simultaneously applying fluazinam and hymexazol, or applying fluazinam and hymexazol separately, or applying fluazinam and hymexazol sequentially.
A bactericidal composition further comprises a filler and/or a surfactant.
The bactericidal composition is used for preventing and controlling fungi and bacteria on cereals, vegetables, fruits, ornamental plants and grapevines.
The use of the fungicidal compositions for the protection of plants, plant propagation material and plant organs that grow at a later time.
The application of the bactericidal composition to the site needing control to control pathogenic or saprophytic fungi and bacteria in soil or culture medium.
The use of the fungicidal composition for the treatment of seeds to protect against attack by phytopathogenic fungi carried by the seeds.
The use of the fungicidal composition for the protection of harvested plants.
The use of the fungicidal composition for protecting stored goods against fungal or bacterial infestation during storage.
The fungicidal compositions are used for controlling fungi in various crop plants such as bananas, cotton, vegetable varieties (e.g. cucumbers, beans, tomatoes and cucurbitaceae), barley, grasses, oats, coffee, potatoes, maize, fruit varieties, rice, rye, soybeans, grapevines, wheat, ornamentals, sugar cane and a large number of seeds.
A method of controlling phytopathogenic fungi of plants, plant propagation material and plant organs which grow subsequently comprises applying the fungicidal composition of the invention in an agronomically effective and substantially non-phytotoxic manner by soaking, dripping, pouring, spraying, dusting, scattering or smoking the plant, the plant propagation material or the soil or cultivation medium in which the plant is growing or in which it is desired to grow.
The fungicidal compositions of the invention can be used as foliar fungicides in crop protection, and also as fungicides for seed dressing and as soil fungicides.
The fungicidal compositions according to the invention have very good fungicidal properties and can be used for controlling phytopathogenic fungi, in particular from the classes of Ascomycetes (Ascomycetes), Basidiomycetes (Basidiomycetes), Phycomycetes (Phycomycetes) and Deuteromycetes (Deuteromycetes).
The bactericidal composition has very good bactericidal performance and can be used for preventing and controlling plant pathogenic bacteria. Such as Pseudomonas (Psedomonas), Rhizobiaceae (Rhizobiaceae), Enterobacter (Enterobacteriaceae), Corynebacterium (Cornebacteriaceae), and Streptomyces (Streptomyces).
By way of example, the following pathogens belonging to the generic names mentioned above, which cause fungal and bacterial diseases, can be mentioned in a non-limiting manner:
as the oomycetes, there may be exemplified, for example, Pythium species such as Rhizoctonia solani (Pythium ultium) of various crops; phytophthora species such as Phytophthora solani (Phytophthora infestans), Botrytis cinerea (Phytophthora capsici); pseudoperonospora species such as Pseudoperonospora cubensis (Pseudoperonospora cubensis), peronospora humuli (Pseudoperonospora humuli); plasmopara species such as Plasmopara viticola; such as Peronospora brassiccus (Peronospora brassicae), Peronospora shallot (Peronospora destructor), Peronospora spinacia (Peronospora spinosae), and the like.
As Ascomycetes, there may be exemplified, for example, Erysiphe species such as Erysiphe graminis (Erysiphe); sphaerotheca species such as vegetable powdery mildew (Sphaerotheca fuliginea); venturia species such as apple scab (Venturia inaqualis), Venturia pear scab (Venturia nashicoloa); pyrenophora species such as barley reticulum (Pyrenophorates); a bacterium belonging to the genus Cochliobolus such as barley speckled disease (Cochliobolus sativus); examples of the species include those belonging to the genus Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), and species belonging to the genus Pyrenophora carried by seeds, such as Pyrenophora gramineus (P.avenae), Pyrenophora avenae (Pyrenophora avenae), Pyrenophora gramineus (Pyrenophora graminea), Pyrenophora teres (Pyrenophora teres), Pyrenophora tritici-cus (Pyrenophora semeniperda), Pyrenophora tritici-repentis, etc.
As the Basidiomycetes, there can be exemplified, for example, a fungus belonging to the genus Septoria such as Puccinia graminis (Septoria nodorum), a fungus belonging to the genus Sclerotium such as Colletotrichum cucumerinum (Colletotrichum lagenarium), a fungus belonging to the genus Pyricularia such as Fusarium oxysporum (Pyricularia oryzae), a fungus belonging to the genus Botrytis such as Botrytis cinerea such as Alternaria alternata (Alternaria mali), a fungus belonging to the genus Alternaria such as Botrytis cinerea (Botrytis cinerea), a fungus belonging to the genus Alternaria such as Nostospora sphaera (Alternaria solani), a fungus belonging to the genus Mycoleptosphaera such as Cladosporium nigra (Rhizoctonia solani), a fungus belonging to the genus Rhizoctonia such as Rhizoctonia solani (Rhizoctonia solani), and the like.
The bactericidal composition also has excellent excitation effect in plants. They are therefore also suitable for mobilizing the in vivo defence system of plants against attack by unwanted phytopathogens.
In the present invention, plant elicitor (resistance-inducing) compounds are understood to be substances which are capable of eliciting a defence system in plants such that, when the treated plants are subsequently inoculated with an undesired phytopathogen, the plants exhibit a significant resistance to the latter.
In the context of the present invention, undesirable phytopathogens are to be understood as meaning phytopathogenic fungi and bacteria. The fungicidal compositions of the present invention are therefore useful for protecting plants against attack by said pathogens for a certain period of time after treatment. The period of protection obtained generally extends from 1 to 10 days, preferably from 1 to 7 days, from the treatment of the plants with the active compounds.
Crops suitable for the fungicidal compositions of the invention include mainly field crops, such as corn, soybean, cotton, canola oil seeds, such as Brassica napus (e.g. canola), turnip (Brassica rapa), Brassica juncea (b.juncea) (e.g. mustard (mustard)) and Brassica carinata (Brassica carinata), rice, wheat, sugar beet, sugar cane, oat, rye, barley, millet, triticale, flax, grapevine and fruit or vegetable crops of various plant classes, such as rosaceae (rosaceae) (e.g. pome fruits, such as apple and pear, and kernel fruits, such as apricot, cherry, almond and peach, berries such as strawberry, tea cane, rosaceae (ribeoiae sp., jugaceae), Juglandaceae (jugaceae), Betulaceae (betula, lac), fagus (fagus), fagus (Oleaceae), fagus Oleaceae (Oleaceae), Brassica Oleaceae (Brassica Oleaceae), Brassica Oleaceae (Oleaceae), fagus Oleaceae (Oleaceae) and fagus Oleaceae (Oleaceae) fruits, Brassica Oleaceae (Brassica, Actinidiaceae (actinodaceae sp.), Lauraceae (Lauraceae sp.), Musaceae (Musaceae sp.), such as banana tree and pink banana (plantains), Rubiaceae (Rubiaceae sp.), such as coffee, Theaceae (Theaceae sp.), firmianaceae (sterculiaceae sp.), Rutaceae (Rutaceae sp.), such as lemon, orange and grapefruit; solanaceae (solanaceae sp.) (e.g. tomatoes, potatoes, peppers, eggplants), Liliaceae (Liliaceae sp.), compositaceae (Compositiae sp.) (e.g. lettuce, artichoke and chicory-including root chicory (root chicory), endive (endive) or common chicory), Umbelliferae (Umbelliferae sp.) (e.g. carrots, parsley, celery and celeries), Cucurbitaceae (Cucurbitaceae sp.) (e.g. cucumbers-including pickled cucumbers (pickling cuumber), squash, watermelons, cucurbits and melons), Alliaceae (Alliaceae sp.) (e.g. onions and leeks), Cruciferae sp. (e.g. white cabbage, red cabbage, broccoli, cabbage, cauliflower, broccoli, brussels sprouts, cabbage, parsley, radish, bean, lentils, beans (bean), lentils, beans, Chenopodiaceae (Chenopodiaceae sp.) (e.g., fodder beet, spinach sweet (spinach beet), spinach, beetroot), Malvaceae (e.g., okra), asparagines (e.g., asparagus); horticultural and forest crops; an ornamental plant; and genetically modified homologues of these crops.
The fungicidal compositions according to the invention are particularly suitable for controlling phytopathogenic fungi from the genera of Erysiphe graminis (Blumeria graminis) (powdery mildew), Rhizoctonia solani (Rhizoctonia solani) (sheath blight), Phytophthora infestans (blight), Pseudomonas sp. (leaf spot), Erysiphe cichoracearum) and Sphaerotheca fuliginosa (Sphaerotheca fuliginosa) in cucurbitaceae, Podospora fuliginosa (Podospora leucotricha) in apple, Rhizoctonia reticulata (Uncaria reticulata) in grapevine, Rhizoctonia (Puccinia) in gramineae, Rhizoctonia (Rhizoctonia) in cotton, rice and turf, Rhizoctonia graminis (Rhizoctonia) in cereals and sugar cane, Phycomyces (Rhizoctonia nigrospora in cereals and sugarcane), Phyllospora nigrospora (Botrytis cinerea) in wheat, Phyllospora Botrytis (Botrytis), Gracilaria graminis (Rhizoctonia) in wheat, Gracilaria solanum Botrytis (Botrytrium graminis) in wheat, Gracilaria (Rhizoctonia), groundnut tail spore (Cercospora arachidicola) in peanuts, eyespot pathogen (pseudocercospora herpotrichoides) in wheat and barley, rice blast pathogen (Pyricularia oryzae) in rice, Phytophthora infestans (Phytophthora infestans) in potatoes and tomatoes, and botrytis (Fulvia) (leaf mold) in tomatoes; plasmopara viticola (Plasmopara viticola) in grapevine, Pseudoperonospora Pseudoperonospora (Pseudoperonospora) in hops and cucumbers, Alternaria (Alternaria) in vegetables and fruits, Mycosphaerella (Mycosphaerella) in bananas, and Neurospora (Fusarium) and Verticillium (Verticillium).
The fungicidal compositions according to the invention are particularly suitable for controlling downy mildew, early blight, late blight, black shank, scab, leaf spot, leaf mold, anthracnose, powdery mildew, wilting, scab, leaf rust, sheath blight, sclerotinia, gray mold, black spot, brown spot, black shank, rust, sclerotinia, clubroot, ring spot, clubroot, bruising, rot, blight, rhizoctonia, and blight of bananas, cotton, vegetable varieties (such as cucumbers, beans, tomatoes, and cucurbitaceae), barley, grasses, oats, coffee, potatoes, maize, fruit varieties, rice, rye, soybeans, grapevines, wheat, ornamentals, sugarcane, and a large number of seeds.
The bactericidal composition can be used as a foliar bactericide, a bactericide for seed dressing and a bactericide for soil for crop protection. In addition, the fungicidal compositions of the present invention are also suitable for controlling harmful fungi present in wood or plant roots.
The present invention also provides a method of controlling phytopathogenic fungi of plants, plant propagation material and stored products of plant organs, plants or plant parts which grow subsequently, comprising applying the fungicidal composition of the invention in an agronomically effective and substantially non-phytotoxic application rate by drenching, instillation, pouring, spraying, misting, dusting, scattering or smoking, to the plants, plant propagation material or to the soil or to a cultivation medium in which the plants are growing or in which it is desired to grow.
The invention provides a method for controlling plant pathogenic bacteria, which is characterized in that a bactericidal composition acts on the pathogenic bacteria and/or the environment thereof, or plants, plant propagation materials, soil, materials or spaces.
The fungicidal compositions of the present invention are useful for the protection of plants, plant propagation material and plant organs that grow at a later time.
The bactericidal composition has good plant tolerance under the concentration required for preventing and treating plant diseases, so that overground parts, in-vitro propagation strains (propathionstock), seeds and soil of plants can be treated. The fungicidal compositions of the present invention may also be applied foliar or used for seed dressing.
Accordingly, the present invention also provides seeds coated with the fungicidal composition of the present invention.
The fungicidal compositions exhibit reduced toxicity and excellent plant tolerance.
All plants and plant parts can be treated according to the invention. Plants are to be understood as meaning all plants and plant populations, for example desired and undesired wild plants or crop plants (including naturally occurring crop plants). Crop plants can be plants which are obtained by conventional plant breeding and optimization methods or by biotechnological and genetic engineering methods or by combinations of these methods, including the transgenic plants, but also including the plant varieties which are protected or not protected by plant breeders' rights. Plant parts are to be understood as meaning all parts of the ground and underground and plant organs of plants, such as shoots, leaves, flowers and roots, examples which may be mentioned being leaves, needles, stems, flowers, fruit bodies, fruits, seeds, roots, tubers and rhizomes. Plant parts also include harvests, as well as vegetative and generative propagation material, such as seedlings, tubers, rhizomes, cuttings and seeds.
The term "plant propagation material" is understood to mean all reproductively competent plant parts, such as seeds, which can be used for the propagation of the latter, and also vegetative materials, such as cuttings or tubers (e.g. potatoes). Thus, plant parts as used herein include plant propagation material. Mention may be made, for example, of seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated plants and useful plants to be inhibited after germination or after emergence from the soil. The young plants can be protected prior to transplantation by a total or partial treatment by dipping.
The plant propagation material may be prophylactically treated with fluazinam and hymexazol or with a composition comprising fluazinam and hymexazol at the time of or before sowing or transplanting.
Preferred propagation material of the present invention is a seed. The fungicidal compositions of the present invention are also particularly suitable for the treatment of seeds. Most of the crop damage caused by harmful fungi is caused by the attack of the seeds during storage or after sowing and during or after germination of the plants. Plants are particularly sensitive to roots and shoots during the growing period and can cause death of the plant even if there is little damage. This stage is particularly critical, since it is very important to protect the seeds and the germinating plants by using suitable compositions.
The control of phytopathogenic fungi by treating the seeds of plants has been the subject of long-term research. Another aspect of the present invention provides a method for protecting seeds and germinating plants, which method makes it unnecessary or at least significant additional application of crop protection agents after sowing or after the emergence of the plants. On the other hand, the amount of active compound used is optimized with the fungicidal composition according to the invention in order to provide maximum protection of the seeds and the germinating plants from attack by phytopathogenic fungi, without the plants themselves being harmed by the active compound used.
The present invention therefore also relates in particular to a method for protecting seeds and germinating plants from attack by phytopathogenic fungi by treating the seeds with a fungicidal composition according to the present invention. The invention also relates to the use of the composition according to the invention for treating seeds to protect the seeds and germinating plants from phytopathogenic fungi. Furthermore, the present invention relates to seeds treated with the fungicidal compositions according to the invention for protection against phytopathogenic fungi. Therefore, the bactericidal composition of the present invention can be used as a seed dressing agent.
The control of phytopathogenic fungi which damage the post-emergent plants is effected primarily by treating the soil and the aerial parts of the plants with crop protection agents. In view of the possible effects of crop protection agents on the environment and on the health of humans and animals, it is therefore necessary to minimize the application rates of the active compounds.
The fungicidal compositions of the invention are suitable for protecting the seed of any plant variety applied in agriculture, in greenhouses, in forestry or in horticulture-or grape cultivars. In particular, it takes the form of seeds of cereals (such as wheat, barley, rye, triticale, millet, oats), maize, cotton, soybean, rice, potato, sunflower, beans, coffee, sugar beet, peanut, rape, olive, cocoa, sugar cane, tobacco, vegetables (such as tomato, cucumber, onion and lettuce), turfgrass and ornamental plants. The treatment of cereal and vegetable seeds is of vital importance.
The active ingredients fluazinam and hymexazol in the fungicidal composition of the present invention are applied to seeds alone or in a suitable formulation. It is preferably treated in a sufficiently stable state that the treatment does not cause any damage to the seed. In general, the treatment of the seeds can be carried out at any point in time between picking and sowing. The seeds commonly used are isolated from the plant and from the cob, husk, stem, cuticle, hair or pulp. Thus, for example, seeds that have been picked, cleaned and dried to a moisture content of less than 15% by weight may be used. Alternatively, seeds may be used which are dried, for example by treatment with water, and then dried again.
Examples of the method of seed treatment include a method of diluting a liquid or solid chemical, a method of directly immersing seeds in a liquid solution without dilution to allow the chemical to permeate the seeds, a method of mixing a solid chemical or liquid chemical with seeds to coat the seeds and thereby adhering the chemical to the surfaces of the seeds, and a method of spraying the chemical to the vicinity of the seeds while planting.
A plant part and plant organ that subsequently grows is any part of a plant produced from plant propagation material, such as seeds. Plant parts, plant organs and plants may also benefit from the pathogenic damage protection obtained by applying the fungicidal composition to plant propagation material. Certain plant parts and plant organs that grow after certain locations may also be considered plant propagation material, which itself may be applied (or treated) with the fungicidal composition; thus plants, other plant parts and other plant organs produced from the treated plant parts and treated plant organs may also benefit from the application of the germicidal composition.
The fungicidal compositions of the present invention may also be used to prevent or control a variety of pathogenic or saprophytic fungi and bacteria in soils or cultivation media. Examples of soil-borne fungal pathogens include Alternaria (Alternaria spp.), Ascochyta (Ascochyta spp.), Botrytis cinerea (Botrytis cinerea, Cercospora spp., ergot (claviceps purpurea), cochliobolus graminis (cochliobolus sativus), colletotrichum (colletotrichum spp.), periplophora sp., fusarium graminearum (fusarium graminearum), Alternaria oryzae (fusarium moniliforme), fusarium oxysporum (fusarium oxysporum), fusarium moniliforme (pisum protuberum), fusarium solani (fusarium solani), streptomyces virescens (fusarium oxysporum), fusarium solani (fusarium solanorum), pyricularia (fusarium oxysporum), pyricularia (rhizophora), pyricularia nigripes (rhizoctonia), pyricularia nigrospora (rhizoctonia), pyricularia (rhizophora), pyricularia nigrospora (rhizoctonia sp.), pyricularia sp., pyricularia (rhizophora niponaria), pyricularia (rhizophora sp., pyricularia niponaria), pyricularia sp., pyricularia nipula (rhizophora sp., pyricularia nipula sp., pyricularia nipula sp., pyricularis, pyricularia sp., pyricularia nipula sp., pyricularia (fusarium sp., pyricularia sp., pyricularis, pyricularia, ramaria (Typhula incarnate), Ubicolo (Urocysticucta), Ustilago (Ustilago spp.) or Verticillium (Verticillium spp.).
The soil germs include rhizoctonia solani, fusarium, phytophthora, damping-off, root rot, pythium, botrytis cinerea, soft rot and the like. Under general conditions, soil pathogenic bacteria can generate a large amount of bacteria, as long as conditions are favorable for growth and development of the pathogenic bacteria and hosts are susceptible, the pathogenic bacteria can propagate in a large amount and infect the hosts, under the host infected with diseases, the pathogenic bacteria can enter a continuous pathogenic period, propagate and diffuse in a large amount along with continuous cropping of crops, but then nutrients are consumed, or when soil conditions such as temperature, humidity and the like are unfavorable for the pathogenic bacteria, the pathogenic bacteria can enter a dormant period. When the host with disease does not exist, soil-borne disease bacteria can survive in soil, and the soil-borne disease bacteria can survive on the root surface or the fallen leaves of the non-host except the soil-borne disease bacteria with wide host range and have the saprophytic competitive ability. However, different germs are different, and like fusarium can almost survive in soil indefinitely.
The culture medium of the present invention refers to a support capable of rooting and growing crops, such as: examples of the raw material include sand, pumice, vermiculite, diatomaceous earth, agar, gel, polymer, asbestos, wood chips, and bark.
Examples of methods for applying a chemical to soil include a method in which a liquid chemical is diluted in water or applied without dilution directly to the roots of a plant or a seedling bed for raising seedlings, a method in which granules are sown to the roots of a plant or a seedling bed for raising seedlings by spraying a powder, a water dispersible granule or the like to soil and mixing with the whole soil before sowing, and a method in which a powder, a water dispersible granule or the like is diluted and sprayed to a planting hole or a planting furrow before sowing or planting a plant, and sowing is performed.
It is another object of the present invention to provide a method of controlling phytopathogenic fungi of plants, parts of plants, plant propagation material and plant organs which grow at a later time, which comprises applying the fungicidal composition of the present invention to the plants, parts of plants, plant propagation material or to the soil or to the cultivation medium in which the plants are growing or in which it is desired to grow, in an agronomically effective and substantially non-phytotoxic manner by seed treatment, foliar application, stem application, drench, drip, pour, spray, mist, dusting, scattering or smoking.
The germicidal compositions of the present invention may be applied by various treatment methods, such as:
-spraying a liquid comprising the fungicidal composition onto the above-ground parts of the plant;
-dusting, incorporating granules or powders in the soil, spraying around said plants and, in the case of tree injection or painting;
-coating or film coating the seeds of the plants.
The present invention provides a method for controlling plant pathogenic bacteria, which can be a treatment, prevention or eradication method.
The fungicidal compositions according to the invention can also be applied during the growth of plants or plant parts in order to protect the harvested plants or plant parts.
According to the invention, post-harvest and storage diseases can be caused, for example, by the following fungi: colletotrichum species, such as banana Colletotrichum (Colletotrichum musae), Colletotrichum disclinae (Colletotrichum gloeosporioides), capsicum Colletotrichum (Colletotrichum coccodes); fusarium species, such as Fusarium semitectum (Fusarium semitectum), Fusarium moniliforme (Fusarium moniliforme), Fusarium solani (Fusarium solani), Fusarium oxysporum (Fusarium oxysporum); verticillium species, such as, for example, Verticillium theobromae (Verticillium theobromae); a species of the genus Neurospora; botrytis species, such as Botrytis cinerea; geotrichum species, such as Geotrichum candidum (Geotrichum candidum); phomopsis species, Phomopsis natalensis (Phomopsis natalensis); species of the genus Lasiosphaera, such as, for example, Dichloropsis citrifolia (Diplodia citri); alternaria species, such as, for example, Alternaria citri (Alternaria citri), Alternaria alternata (Alternaria alternata); phytophthora species, such as Phytophthora citri (Phytophthora citrophthora), Phytophthora fragrans (Phytophthora fragaria), Phytophthora infestans (Phytophthora cactorum), Phytophthora nicotiana (Phytophthora parasitica), Septoria (Septoria spp.), such as Septoria depressa; mucor spp, such as Mucor piriformis (Mucor piriformis); streptomyces (Monilinia spp.), such as, for example, Streptomyces fructicola (Monilinia fructicola), Streptomyces drupes (Monilinia laxa); venturia spp, such as Venturia inaequalis, Venturia pyrifera (Venturia inaegulis), Venturia pyrifolia (Venturia pyrina); rhizopus sp, such as Rhizopus stolonifer, Rhizopus oryzae (Rhizopus oryzae); genus Microtheca (Glomerella spp.), e.g., Percocephala (Glomerella cingulata); sclerotinia spp, such as Sclerotinia fructicola (Sclerotinia fructicola); the genus longbeak (Ceratocystis spp.), such as the Kiwi long beak (Ceratocystis paradoxa); penicillium spp, such as Penicillium funiculosum (Penicillium funiculosum), Penicillium expansum (Penicillium expandasum), Penicillium digitatum (Penicillium digitatum), Penicillium italicum (Penicillium italicum); pediophora sp, e.g., Pediophora albuginea (Gloeosporium album), Gloeosporium perennans, Pediophora fructicola (Gloeosporium fructigenum), Gloeosporium singulata; humularia (Phlyctaenana spp.) such as Phlyctaena vagabunda; cylindrocarpon spp, such as, for example, Cylindrocarpon mali; stemphylium spp, such as stemphylium citrinum (stemphylium venelicanum); aschersonia (Phacydiopanis spp.), e.g., Phacydiopani malirum; rhizopus spp, such as Rhizopus mirabilis paradoxy; aspergillus spp, such as Aspergillus niger, Aspergillus carbonarius, Nectria spp, e.g. Nectria cancerosa (Nectria galagena); amycolatopsis (Pezicula spp.).
The treatment method according to the invention can also be used to protect stored products from fungal and microbial attack. According to the invention, the term "stock" is understood to mean natural substances and processed forms thereof of plant or animal origin which have been derived from the natural life cycle and are intended to be preserved for a long period of time. Storage products of plant origin, for example plants or parts thereof, such as stems, leaves, tubers, seeds, fruits or grains, can be protected in the freshly harvested state or in processed form, such as (pre) drying, wetting, comminuting, grinding, pressing or baking. Or wood, in the form of coarse wood such as construction timber, utility poles and fences; or in finished form, such as furniture or articles made of wood. The animal-derived stock is hide, leather, hair, etc. The compositions according to the invention can prevent fungal or bacterial attack such as corrosion, discoloration or mildew on storage. "stock" is preferably understood as meaning natural substances of plant origin and processed forms thereof, more preferably fruits and processed forms thereof, such as pomes, stone fruits, stone-free small fruits and citrus fruits and processed forms thereof.
The fungicidal composition of the present invention can be used in the form of a conventional formulation, for example, emulsifiable concentrate, aqueous suspension, oil suspension, dry powder for seed treatment, suspension for seed treatment, water dispersible granule, wettable powder, suspoemulsion, aerosol, coated granule, extruded granule, emulsion in water, microcapsule suspension-suspension, dry suspension, ultra-low volume liquid, electrostatic oil, gel, and the like, and the application amount thereof varies depending on the mixing ratio of the active ingredients, weather conditions, formulation, application period, application method, application place, control of harmful fungi, target crops, and the like.
A method for preventing and treating plant pathogenic bacteria comprises simultaneously applying fluazinam and hymexazol, or applying fluazinam and hymexazol separately, or applying fluazinam and hymexazol sequentially.
The treatment according to the invention may produce a synergistic effect. For example, depending on the application rate and/or broadening the activity spectrum and/or increasing the activity of the fungicidal compositions used according to the invention, it is possible to obtain the following effects: better plant growth, increased tolerance to high or low temperatures, increased tolerance to drought or to water or soil salt content, improved flowering performance, easier harvesting, accelerated ripening, higher harvest yields, larger fruits, higher plant height, greener leaf color, earlier flowering, higher quality or nutritional value of the harvested product, higher sugar concentration in the fruit, better storage stability and/or processability of the harvested product, which exceeds the effects that are actually predicted.
The treatment method of the invention may also be used to treat propagation material such as tubers or rhizomes, and may be used to treat seeds, seedlings or transplanted (packing out) seedlings and plants or transplanted plants. This processing method can also be used to process roots. The treatment method of the present invention can also be used for treating the above-ground parts of plants such as the stems, stems or stalks, leaves, flowers and fruits of the plants concerned.
For leaf treatment in general: 0.1 to 10000g/ha, preferably 10 to 1000g/ha, more preferably 50 to 500 g/ha; more preferably 50 to 300 g/ha; for dipping or instillation administration, the dosage may even be reduced, particularly when an inert substrate such as asbestos or perlite is applied;
for seed treatment: 2-5000g/100kg of seeds, preferably 3-1000g/100kg of seeds, preferably 10-500 g/100kg of seeds, more preferably 10-300 g/100kg of seeds, more preferably 10-150g/100kg of seeds;
for soil or water surface application treatments: 0.1 to 10000g/ha, preferably 1 to 1000 g/ha.
The above-mentioned dosages are only typical exemplary dosages, and the person skilled in the art will adjust the application rate in the actual application according to the actual circumstances and needs, in particular according to the nature of the plants or crops to be treated and the severity of the germs.
The fungicidal composition of the present invention can be converted into conventional formulations such as emulsifiable concentrates, aqueous suspensions, oil suspensions, dry powders for seed treatment, suspension for seed treatment, water dispersible granules, wettable powders, suspoemulsions, aerosols, coated granules, extruded granules, aqueous emulsions, microcapsule suspensions, microcapsule suspension-suspensions, dry suspensions, ultra-low volume liquids, electrostatic oils, gels, by known methods to produce these formulations, for example, by mixing an active compound with a filler such as a liquid or liquefied gas or a solid diluent or carrier, optionally using a surfactant such as an emulsifier, a dispersant, and/or a foam-forming agent. In the case of water as filler, it is also possible to use organic solvents, for example organic solvents as co-solvents.
The liquid diluent or carrier is typically: aromatic compounds such as xylene, toluene or alkylnaphthalenes, chlorinated aromatic compounds or chlorinated aliphatic hydrocarbons such as chlorobenzene, vinyl chloride or dichloromethane, aliphatic hydrocarbons such as cyclohexane or paraffins such as petroleum fractions, mineral and vegetable oils, alcohols such as butanol or ethylene glycol and their ethers and esters, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, strongly polar solvents such as dimethylformamide and dimethyl sulfoxide, or water.
Suitable solid carriers, as for dusts and water dispersible granules, are: for example ammonium salts and crushed natural minerals, such as kaolin, clay, talc, chalk, quartz, attapulgite, montmorillonite or silicates, and crushed synthetic minerals, such as highly dispersed silica, alumina and silicates.
Solid carriers which may be used are particles of natural minerals, such as kaolin, clay, talc, chalk, quartz, attapulgite, montmorillonite or kieselguhr, and particles of synthetic minerals, such as highly dispersed silicic acid, alumina and silicates.
Solid carriers which can be used for granules are crushed and fractionated natural rocks such as calcite, marble, pumice, sepiolite and dolomite, as well as inorganic synthetic granules and organic powders, and granules of organic products such as sawwood, coconut shells, maize cobs and tobacco stalks.
Examples of the surfactant that can be used for emulsifying, dispersing, solubilizing, and/or wetting the active compound include polyacrylic acid salts such as fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid ester, phosphoric acid ester of polyoxyethylene alcohol or phenol, fatty acid ester of polyhydric alcohol, alkaryl sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonate, branched polymer of high molecular comb, butyl naphthalene sulfonate, alkylaryl sulfonate, sodium alkylsulfosuccinate, fats and oils, condensation product of fatty alcohol and ethylene oxide, and alkyltaurate, and protein hydrolysates. Suitable oligosaccharides or polymers are based, for example, on ethylene monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with, for example, (poly) alcohols or (poly) amines.
Tackifiers such as carboxymethylcellulose and natural and synthetic polymers in the form of powders, granules or latices, such as gum arabic, polyvinyl alcohol and polyvinyl acetate, or else natural phospholipids, such as cephalins and lecithins, and synthetic phospholipids, can be used in the formulations. Other additives are mineral oils and vegetable oils.
It is possible to use colorants such as inorganic pigments, for example iron oxide, titanium oxide and Prussian blue, and organic dyes, such as alizarin dyes, azo dyes or metal phthalocyanine dyes, and trace nutrients, such as salts of iron, manganese, boron, ketones, cobalt, molybdenum and zinc.
Optionally, further additional components, such as protective colloids, binders, thickeners, thixotropic agents, penetrating agents, stabilizers, masking agents, can also be included.
The formulations of the invention can be prepared by mixing the active compounds with the customary additives in a known manner. Such as conventional extenders as well as solvents or diluents, emulsifiers, dispersants, and/or binders or fixatives, wetting agents, water repellents, if desired siccatives and colorants, stabilizers, pigments, defoamers, preservatives, thickeners, water and other processing aids.
The fungicidal compositions of the present invention may also be applied in combination with other active ingredients, for example to broaden the spectrum of activity or to prevent the development of resistance. Such as fungicides, bactericides, attractants, insecticides, acaricides, nematicides, growth regulators, herbicides, safeners, fertilizers or semiochemicals and the like. The combined administration can be present as a premix or, if appropriate, mixed just before administration (tank mix).
The user typically applies the germicidal composition of the present invention from a front-dose device, a backpack sprayer, a spray can, or a spraying airplane. The bactericidal composition of the present invention is formulated with water and/or a buffer to a desired application concentration, or directly applied to give a spray liquid.
The fungicidal compositions of the present invention may also be used to improve plant health. The invention therefore also relates to a method of improving the health of a plant by treating the plant, plant propagation material, the soil or cultivation medium in which the plant is growing or is in need of growing with an effective amount of a fungicidal composition of the invention.
The term "plant health" is understood to mean the condition of a plant and/or its products as determined by various indications such as yield (e.g. increased biomass and/or increased content of valuable ingredients), plant vigour (e.g. improved plant growth and/or greener leaves, quality (e.g. increased content or composition of certain ingredients) and tolerance to non-life and/or vitality, alone or in combination with each other.
Fluazinam and hymexazol are applied directly or in the form of a composition by treating the fungus or the plant, plant propagation material such as seeds, soil, surfaces or spaces to be protected from fungal attack with a fungicidally effective amount of the active ingredients. Application can be carried out before and after the infestation of the plants, plant propagation material, such as seeds, soil, surfaces, materials or spaces, by the fungi.
The sterilization composition has the beneficial effects that:
the invention provides a bactericidal composition, which combines fluazinam and hymexazol, so that the obtained composition has a synergistic effect on prevention and treatment effects, widens the bactericidal spectrum, and effectively slows down or avoids the generation of drug resistance of germs. Surprisingly, the fungicidal activity of the fungicidal compositions according to the invention is significantly higher than the sum of the activities of the individual active compounds; there is an unpredictable, truly present synergistic effect, not just a supplementation of activity.
The synergistic effect is particularly pronounced when the active compounds are present in the fungicidal compositions of the present invention in a particular weight ratio. However, the weight ratio of the active compounds in the fungicidal compositions according to the invention can vary within certain limits.
Detailed Description
Example 120% fluazinam +40% hymexazol wettable powder
20 percent of fluazinam
Hymexazol 40%
Sodium dodecyl sulfate 10%
Sodium lignosulfonate 5%
White carbon black 10%
Kaolin to make up to 100%.
Mixing the active ingredients, various auxiliary agents, fillers and the like according to the proportion of the formula, and crushing the mixture by using an ultrafine crusher to obtain 20 percent fluazinam and 40 percent hymexazol wettable powder.
Example 20.5% fluazinam +1% hymexazol powder
0.5 percent of fluazinam
1 percent of hymexazol
1 percent of calcium dodecyl benzene sulfonate
Sodium lignosulfonate 2%
The high top soil is complemented to 100 percent.
The active ingredients, various auxiliary agents, fillers and the like are mixed according to the proportion of the formula and crushed by a superfine crusher to obtain the 0.5 percent fluazinam and 1 percent hymexazol dusting composition which can be directly applied without dilution.
Example 31% fluazinam +1% hymexazol ultra-low volume liquid formulation
1 percent of fluazinam
1 percent of hymexazol
Ethoxylated castor oil 5%
Methyl oleate makes up to 100%.
The components are prepared according to the proportion and are stirred uniformly to obtain a phase. The resulting composition was applied directly without dilution.
Example 40.2% fluazinam +0.3% hymexazol granule
0.2 percent of fluazinam
0.3 percent of hymexazol
Sodium dodecyl sulfate 5%
Kaolin to make up to 100%.
The components are ground and granulated by an extrusion, spray drying or fluidized bed method to obtain the granules which are applied without dilution and have the content of 0.2 percent of fluazinam and 0.3 percent of hymexazol.
Example 510% fluazinam +40% hymexazol Water dispersible granules
10 percent of fluazinam
Hymexazol 40%
5 percent of modified calcium lignosulfonate
Sodium dodecyl sulfate 5%
5 percent of urea
Kaolin to make up to 100%.
Uniformly mixing fluazinam, hymexazol active ingredients, a dispersing agent, a wetting agent, a disintegrating agent and a filler according to the proportion of a formula, and performing air flow crushing to obtain wettable powder; adding a certain amount of water, mixing and extruding to obtain the finished product. And drying and screening to obtain the 10% fluazinam and 40% hymexazol water dispersible granule.
Example 650% fluazinam +20% hymexazol suspoemulsion
Oil phase:
20 percent of hymexazol
Oleic acid methyl ester 10%
Ethoxylated castor oil 5%
Water phase:
50 percent of fluazinam
Sodium salt of sulfonated naphthalene sulfonic acid-formaldehyde condensation product 1%
Water is added to make up to 100%.
Dissolving hymexazol in methyl oleate, and adding ethoxylated castor oil to obtain an oil phase; grinding and/or shearing the fluazinam, the sodium salt of the sulfonated naphthalene sulfonic acid-formaldehyde condensation product and water at a high speed according to a formula to obtain the fluazinam water suspension agent; the oil phase was added to the aqueous phase under stirring to obtain a suspoemulsion.
Example 720% fluazinam +40% hymexazol wettable powder
20 percent of fluazinam
Hymexazol 40%
1 percent of sodium lignosulfonate
Sodium lauryl sulfate 2%
1% of highly dispersed silicic acid
Kaolin to make up to 100%.
The components are mixed according to a proportion, ground and crushed to prepare the wettable powder.
Example 820% fluazinam +50% hymexazol coated granules
20 percent of fluazinam
50 percent of hymexazol
3 percent of polyethylene glycol
1% of highly dispersed silicic acid
Calcium carbonate to 100%.
The finely ground active ingredient is uniformly coated onto the carrier moistened with polyethylene glycol in a mixer. In this way dust-free coated granules are obtained.
Example 920% fluazinam +10% hymexazol wettable powder
20 percent of fluazinam
10 percent of hymexazol
Sodium dodecyl sulfate 1%
1 percent of sodium lignosulfonate
Kaolin to make up to 100%.
The components are mixed according to a proportion, ground and crushed to prepare the wettable powder.
Example 1030% fluazinam +30% hymexazol extruded granules
Fluazinam 30%
30 percent of hymexazol
Sodium lignosulfonate 4%
2 percent of carboxymethyl cellulose
Kaolin to make up to 100%.
The active ingredient is mixed with the auxiliaries and milled, the mixture being moistened with water. The mixture was extruded and then dried in an air stream.
Example 111% fluazinam +2% hymexazol suspension seed coating
1 percent of fluazinam
2 percent of hymexazol
5 percent of disodium fatty alcohol polyoxyethylene ether sulfosuccinate monoester
5 percent of modified calcium lignosulfonate
3 percent of bentonite
Soybean oil was made up to 100%.
The components are mixed according to a certain proportion and ground and/or sheared at a high speed to obtain the suspended seed coating agent.
Example 1210% fluazinam +20% hymexazol microcapsule suspension
ATLOX TM4913 4%
0.05 percent of citric acid
0.1 percent of catalyst
10 percent of water
20 percent of hymexazol
PAPI 1.35%
SOLVESSOTM200 10%
ATLOXTM4913 10%
0.3 percent of dispersant LFH
0.16 percent of defoaming agent
5 percent of urea
10 percent of fluazinam
Water is added to make up to 100%.
Mixing polymethylene polyphenyl polyisocyanate (PAPI), hymexazol, and SolvessoTM200 into oil phase containing ATLOX TM4913 in an aqueous solution, an emulsion is formed. Then heating and maintaining the temperature at 50 DEGoAdding catalyst to react for 2 h. Cooling to obtain the hymexazol microcapsule.
ATLOX TM4913 mixing dispersant LFH, defoamer, urea, fluazinam and water in proportion, grinding and/or high-speed shearing to obtain uniform suspension containing fluazinam.
And adding the obtained hymexazol-containing microcapsule into a fluazinam suspending agent, and uniformly stirring to obtain a 10% fluazinam +20% hymexazol-containing microcapsule suspending agent.
Example 130.5% fluazinam +2.5% hymexazol emulsifiable concentrate
0.5 percent of fluazinam
2.5 percent of hymexazol
Ethoxylated castor oil 5%
Calcium dodecyl benzene sulfonate 3%
SOLVESSOTM200 supplementSufficient to 100 percent.
Mixing the above components, and stirring to obtain transparent homogeneous phase.
Example 1410% fluazinam +30% hymexazol Water dispersible granules
10 percent of fluazinam
30 percent of hymexazol
5 percent of modified calcium lignosulfonate
Sodium dodecyl sulfate 5%
5 percent of urea
Kaolin to make up to 100%.
Uniformly mixing fluazinam, hymexazol active ingredients, a dispersing agent, a wetting agent, a disintegrating agent and a filler according to the proportion of a formula, and performing air flow crushing to obtain wettable powder; adding a certain amount of water, mixing and extruding to obtain the finished product. And drying and screening to obtain the 10% fluazinam and 30% hymexazol water dispersible granule.
Example 155% fluazinam +15% hymexazol wettable powder
5 percent of fluazinam
15 percent of hymexazol
Sodium dodecyl sulfate 1%
1 percent of sodium lignosulfonate
Kaolin to make up to 100%.
The components are mixed according to a proportion, ground and crushed to prepare the wettable powder.
Example 165% fluazinam +10% hymexazol gel
5 percent of fluazinam
10 percent of hymexazol
Sodium dodecyl sulfate 5%
Gelling agent 1%
Water is added to make up to 100%.
The above components were ground in a stirred ball mill to obtain a fine suspension. Dilution with water gave a stable suspension of the active ingredient, thus giving a 5% fluazinam +10% hymexazol gel.
Example 1750% fluazinam +10% hymexazol suspension
50 percent of fluazinam
10 percent of hymexazol
10 percent of fatty alcohol-polyoxyethylene ether disodium sulfosuccinate monoester
5 percent of modified calcium lignosulfonate
Xanthan gum 1%
1 percent of bentonite
Glycerol 5%
PVP-K30 1%
Water is added to make up to 100%.
The suspending agent is obtained by mixing the components in proportion and grinding and/or high-speed shearing.
Example 185% Fluazinam +25% hymexazol oil suspension
5 percent of fluazinam
Hymexazol 25%
Sodium dibutylnaphthalenesulfonate 5%
5 percent of modified calcium lignosulfonate
Xanthan gum 1%
1 percent of bentonite
Glycerol 5%
PVP-K30 1%
5 percent of water
Soybean oil was made up to 100%.
The suspending agent is obtained by mixing the components in proportion and grinding and/or high-speed shearing.
Example 192% fluazinam +10% hymexazol Electrostatic finish
2 percent of fluazinam
10 percent of hymexazol
Calcium dodecyl benzene sulfonate 5%
N-methylpyrrolidone to 100%.
The components are mixed and stirred evenly to obtain a uniform phase.
Example 2020% fluazinam +80% hymexazol
20 percent of fluazinam
Hymexazol 80%
The fluazinam and the hymexazol are uniformly mixed according to the proportion.
Example 2140% fluazinam +60% hymexazol
40 percent of fluazinam
Hymexazol 60%
The fluazinam and the hymexazol are uniformly mixed according to the proportion.
The proportion in the above examples is weight percent.
Biological test example:
and (3) testing toxicity:
calculating the virulence index of each medicament and the cotoxicity coefficient (CTC value) of the mixture by a Sun Yunpei method, wherein when the CTC is less than or equal to 80, the composition shows antagonism, when the CTC is less than 80 and less than 120, the composition shows additivity, and when the CTC is more than or equal to 120, the composition shows synergism.
Observed virulence index (ATI) = (standard agent EC 50/test agent EC50) × 100
Theoretical virulence index (TTI) = a agent virulence index% percentage of a in a mixture + B agent virulence index% percentage of B in a mixture
Co-toxicity coefficient (CTC) = [ mix observed virulence index (ATI)/mix theoretical virulence index (TTI) × 100.
Test 1: virulence determination/seed treatment of rhizoctonia solani (rice)
The active compounds are applied as dry seed dressings. For seed dressing, infested seeds and seed dressing agents were shaken for 3 minutes in a closed glass flask. 3 x 100 rice seeds were sown in standard soil to a depth of 1 cm. At a temperature of about 18 deg.CoC. Culturing in a greenhouse with air humidity of 60-70%, and illuminating the seed cultivation box for 12 hours every day. After 3 weeks of sowing, the rice seedlings were evaluated for disease. Then, the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun cloud Pepper method.
TABLE 1 virulence determination/seed treatment of Rhizoctonia solani (rice)
As can be seen from Table 1, when the weight ratio of fluazinam to hymexazol is in the range of 100:1-1:100, the cotoxicity coefficient of Rhizoctonia solani (rice) is greater than 120, which indicates that the mixing of fluazinam and hymexazol in the range shows a gain effect.
Test 2: virulence determination/seed treatment of fusarium flavum (wheat)
The active compounds are applied as dry seed dressings. For seed dressing, infested seeds and seed dressing agents were shaken for 3 minutes in a closed glass flask. 3 x 100 wheat seeds were sown in standard soil to a depth of 1 cm. At a temperature of about 18 deg.CoC. Culturing in a greenhouse with air humidity of 60-70%, and illuminating the seed cultivation box for 13 hours every day. After 3 weeks of sowing, the disease of the wheat seedlings was evaluated. Then, the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun cloud Pepper method.
TABLE 2 toxicity test results of the present invention against Fusarium flavum
As can be seen from Table 2, when the weight ratio of fluazinam to hymexazol is in the range of 100:1-1:100, the co-toxicity coefficient to Fusarium flavum is greater than 120, which indicates that the blending of fluazinam and hymexazol in the range shows a gain effect.
Test 3: virulence determination of downy mildew (cucumber)
Selected from consistent cucumber shoots, sprayed at 50PSI pressure using a potter spray tower, approximately 5mL per pot, with 5 concentration gradients set for each dose. Inoculating the strain 24h after the medicament treatment, inoculating the seedling with a spore suspension of downy mildew, and then putting the cucumber seedling into a greenhouse for culturing. The effect evaluation is carried out 7 days after inoculation, then the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun Yunpei method.
TABLE 3 virulence test results for downy mildew (cucumber) according to the invention
As can be seen from Table 3, when the weight ratio of fluazinam to hymexazol is in the range of 100:1-1:100, the co-toxicity coefficient to downy mildew (cucumber) is more than 120, which indicates that the gain effect is shown by the blending of fluazinam and hymexazol in the range.
Test 4: virulence determination of Botrytis cinerea (tomato)
Selected from tomato seedlings of uniform growth, sprayed with a potter spray tower at 50PSI pressure, approximately 5mL per pot, with 5 concentration gradients set for each dose. Inoculating the strain 24h after the medicament treatment, inoculating the seedling with a spore suspension of botrytis cinerea, and then putting the tomato seedling into a greenhouse for culturing. The effect evaluation is carried out 7 days after inoculation, then the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun Yunpei method.
TABLE 4 toxicity test results of the present invention against Botrytis cinerea (tomato)
As can be seen from table 4, when the weight ratio of fluazinam to hymexazol is in the range of 100:1 to 1:100, the co-toxicity coefficient to gray mold (tomato) is greater than 120, which indicates that the mixing ratio of fluazinam to hymexazol is in this range, and both the fluazinam and the hymexazol show a gain effect.
Test 5: virulence determination/soil treatment of pythium spp (rice)
Soil contaminated with pythium and a predetermined amount of the chemical were mixed in a plastic pot, and then seeds of rice were sown. The disease of the rice seedlings was evaluated 30 days after the start of sowing. Then, the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun cloud Pepper method.
TABLE 5 toxicity test results of the present invention against Pythium species (rice)
As can be seen from Table 5, when the weight ratio of fluazinam to hymexazol is in the range of 100:1-1:100, the co-toxicity coefficient to Pythium species (rice) is greater than 120, which indicates that the synergistic effect is exhibited by the blending of fluazinam and hymexazol in the range.
Test 6 virulence assay for Phytophthora infestans (Potato)
Selected from uniformly grown potato seedlings, sprayed at 50PSI pressure using a potter spray tower, approximately 5mL per pot, with 5 concentration gradients set for each dose. Inoculating the strain 24h after the medicament treatment, inoculating the seedling with a spore suspension of phytophthora infestans, and then putting the potato seedling into a greenhouse for culturing. The effect evaluation is carried out 7 days after inoculation, then the inhibitory intermediate concentration EC50 is calculated by the least square method, and the cotoxicity coefficient (CTC) is calculated by the Sun Yunpei method.
TABLE 6 virulence test results for Phytophthora infestans (potatoes) according to the invention
As can be seen from Table 6, when the weight ratio of fluazinam to hymexazol is in the range of 100:1-1:100, the co-toxicity coefficient to Phytophthora infestans (potatoes) is greater than 120, which indicates that the mixing ratio of fluazinam to hymexazol in the range shows a gain effect.
Claims (21)
1. A germicidal composition, characterized by: the active ingredients comprise fluazinam and hymexazol, and the weight percentage of the fluazinam to the hymexazol is 100:1-1: 100.
2. The germicidal composition of claim 1, wherein: the weight percentage of the fluazinam and the hymexazol is 50:1-1: 50.
3. The germicidal composition of claim 1, wherein: the weight percentage of the fluazinam and the hymexazol is 40:1-1: 40.
4. The germicidal composition of claim 1, wherein: the weight percentage of the fluazinam and the hymexazol is 35:1-1: 35.
5. The germicidal composition of claim 1, wherein: the weight percentage of the fluazinam and the hymexazol is 20:1-1: 20.
6. The germicidal composition of claim 1, wherein: the sum of the mass of the fluazinam and the hymexazol accounts for 0.5-90% of the mass of the sterilization composition.
7. The germicidal composition of claim 1, wherein: the sum of the mass of the fluazinam and the hymexazol accounts for 10-80% of the mass of the sterilization composition.
8. The germicidal composition of claim 1, wherein: the sum of the mass of the fluazinam and the hymexazol accounts for 20-60% of the mass of the sterilization composition.
9. The germicidal composition of claim 1, wherein: the bactericidal composition is in the dosage forms of missible oil, aqueous suspension, oil suspension, seed treatment dry powder, seed treatment suspension, water dispersible granules, wettable powder, suspoemulsion, aerosol, coated granules, extruded granules, aqueous emulsion, microcapsule suspension-suspension, ultra-low volume liquid, electrostatic oil and gel.
10. The germicidal composition of claim 1, wherein: the germicidal composition also includes a filler and/or a surfactant.
11. The germicidal composition of claim 1, wherein the germicidal composition is applied directly or after dilution with water.
12. A method of controlling phytopathogenic fungi, characterized by: the method of treating a pathogen and/or its environment, or a plant, seed, soil, material or space with the germicidal composition of claim 1.
13. The method of claim 12, wherein: the fluazinam and hymexazol to be applied simultaneously, or separately, or sequentially.
14. Use of the fungicidal composition according to claim 1 for controlling fungi.
15. Use of the fungicidal composition according to claim 1 for the control of fungi on cereals, vegetables, fruits, ornamentals and vines.
16. Use of the fungicidal composition according to claim 1 for the protection of plants, plant propagation material and plant organs that grow at a later time.
17. Use of the fungicidal composition of claim 1 for controlling pathogenic or saprophytic fungi in soil or cultivation media, applied to the locus where control is desired.
18. Use of the fungicidal composition according to claim 1 for the treatment of seeds to protect the seeds from attack by phytopathogenic fungi.
19. Use of the fungicidal composition of claim 1 for the protection of harvested plants.
20. Use of the fungicidal composition of claim 1 for protecting stored products from fungal infestation during storage.
21. A method of controlling phytopathogenic fungi of plants, plant parts, plant propagation material and plant organs which grow subsequently, characterized in that: the method comprises applying the fungicidal composition of claim 1 in an agronomically effective and substantially non-phytotoxic application rate to a plant, plant propagation material, or soil or culture medium in which the plant is growing or in which it is desired to grow, by means of saturation, drip, pour, spray, dusting, scattering, or fuming.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067858A (en) * | 2011-01-18 | 2011-05-25 | 河北省农林科学院植物保护研究所 | Compound bactericide for mainly treating grey mold of fruits and vegetables |
| CN104904733A (en) * | 2014-03-11 | 2015-09-16 | 江苏龙灯化学有限公司 | Method for protecting plants from plant diseases |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102067858A (en) * | 2011-01-18 | 2011-05-25 | 河北省农林科学院植物保护研究所 | Compound bactericide for mainly treating grey mold of fruits and vegetables |
| CN104904733A (en) * | 2014-03-11 | 2015-09-16 | 江苏龙灯化学有限公司 | Method for protecting plants from plant diseases |
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