CN111727968A

CN111727968A - Bactericidal composition

Info

Publication number: CN111727968A
Application number: CN202010661286.3A
Authority: CN
Inventors: 罗昌炎; 詹姆斯.T.布里斯托
Original assignee: Jiangsu Rotam Chemical Co Ltd
Current assignee: Jiangsu Rotam Chemical Co Ltd
Priority date: 2016-01-20
Filing date: 2016-01-20
Publication date: 2020-10-02
Also published as: CN106982853A

Abstract

The present invention relates to a germicidal composition; the invention also relates to a method for controlling harmful fungi by using the sterilization composition. The bactericide composition contains active ingredients of meptyldinocap and iprodione, and the weight ratio of the meptyldinocap to the iprodione is 50:1-1: 50. The invention also provides a method of controlling phytopathogenic fungi of plants, plant parts, plant propagation material and plant organs which grow subsequently.

Description

Bactericidal composition

The invention relates to a divisional application with application number 2016100349539, application date 2016, 1, 20 and title "a bactericidal composition".

Technical Field

The present invention relates to a germicidal composition; the invention also relates to a method for controlling harmful fungi by using the sterilization composition.

Background

With regard to the activity of pesticides, in particular with regard to crop protection, one of the core problems of the research carried out in this technical field is the improvement of the properties, in particular in terms of biological activity, and the maintenance of this activity over a certain period of time.

It is an object of the present invention to provide compositions having improved activity against harmful fungi with a reduced total amount of active compounds applied (synergistic compositions), in order to reduce the application rates of known active compounds and to improve their activity spectrum.

Meptyldinocap (Meptyldinocap); CAS: 131-72-6; the chemical formula is: 2, 4-dinitro-6- (1-methylheptyl) benzene crotonate; the molecular structural formula is:

the nitrophenyl phosphate is a bactericide developed by Yinong Dorstaki, has triple functions of protecting, treating and eradicating, and is a specific medicament for powdery mildew.

Iprodione (Iprodione) is a dicarboximide bactericide developed by Bayer corporation, and has a molecular formula of C₁₃H₁₃Cl₂N₃O₃The chemical name 3- (3, 5-dichlorophenyl) -N-isopropyl-2, 4-dioxoimidazoline-1-carboxamide is disclosed in US 3755350. The structural formula is as follows:

iprodione is a broad-spectrum contact bactericide and is mainly used for preventing and treating gray mold of crops such as fruit trees, vegetables, melons and fruits. It inhibits protein kinases, intracellular signals that control many cellular functions, including interference from carbohydrate binding into fungal components. Therefore, it can inhibit the germination and production of fungal spore and the growth of hypha. Namely, the fungicide has influence on all development stages in the life history of pathogenic bacteria and can prevent and treat fungi with resistance to benzimidazole systemic bactericides. The main control objects are various diseases of crops, fruit trees and fruits in the storage period caused by botrytis, monilia, penicillium, sclerotinia, alternaria, helminthosporium and the like.

Since the environmental and economic requirements for fungicides are now constantly increasing, for example with regard to the spectrum of activity, toxicity, selectivity, application rate, residue composition and advantageous production feasibility, and also since problems can occur, for example, with regard to resistance, the development of new fungicides which are superior in some respects to existing fungicides is a constant task.

Disclosure of Invention

Furthermore, we have found that the simultaneous, i.e. joint or separate, application of meptyldinocap and iprodione, or the sequential application of meptyldinocap and iprodione, allows better control of harmful fungi than the application of each compound alone.

The invention provides a bactericidal composition, which enables an obtained mixture to have a gain effect on a prevention and treatment effect by binary compounding of meptyldinocap and iprodione, expands a bactericidal spectrum, and effectively slows down or avoids the generation of drug resistance of germs. Surprisingly, the fungicidal activity of the fungicidal compositions according to the invention is significantly higher than the sum of the activities of the individual active compounds; there is an unpredictable, truly present synergistic effect, not just a supplementation of activity.

The synergistic effect is particularly pronounced when the active compounds are present in the fungicidal compositions of the present invention in a particular weight ratio. However, the weight ratio of the active compounds in the fungicidal compositions according to the invention can vary within certain limits.

The invention discloses a sterilization composition, which is realized by adopting the following technical scheme:

a germicidal composition, characterized by: contains active ingredients of meptyldinocap and iprodione, wherein the weight percentage of the meptyldinocap and the iprodione is 50:1-1:50, preferably 25:1-1:25, 10:1-1:10, and further preferably 5:1-1: 5.

The synergistic effect of the meptyldinocap and the iprodione is particularly obvious in the proportioning range.

The weight ratio of meptyldinocap and iprodione in the present invention may be, for example, 50:1, 45:1, 40:1, 35:1, 30:1, 25:1, 20:1, 15:1, 10:1, 5:1, 1:5, 1:10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1: 50.

The sum of the mass of the meptyldinocap and the iprodione in the bactericidal composition accounts for 5% -90%, more preferably 10% -80%, and still more preferably 20% -60% of the mass of the bactericidal composition.

The bactericidal composition further comprises a filler and/or a surfactant.

A method for preventing and treating plant pathogenic bacteria comprises applying bactericidal composition to pathogenic bacteria and/or its environment, or plant, plant part, seed, soil, material or space.

A method for preventing and treating plant pathogenic bacteria comprises simultaneously applying meptyldinocap and iprodione, or applying them separately or sequentially.

A bactericidal composition comprises meptyldinocap, iprodione, a filler and/or a surfactant.

A bactericidal composition can be prepared into any agriculturally allowable dosage form. The dosage form of the bactericidal composition is suspending agent, seed coating agent, suspoemulsion, wettable powder, water dispersible granules, microcapsule suspending agent, coated granules, extruded granules, missible oil, microemulsion, emulsion in water, effervescent tablets and ultra-low volume liquid.

The bactericidal composition is used for preventing and controlling fungi and bacteria on cereals, vegetables, fruits, ornamental plants and grapevines.

The use of the fungicidal compositions for the protection of plants, plant parts, plant propagation material and plant organs that grow at a later time.

The application of the bactericidal composition to the site needing control to control pathogenic or saprophytic fungi and bacteria in soil or culture medium.

The use of the fungicidal composition for the protection of harvested plants or plant parts.

The use of the fungicidal composition for protecting stored goods against fungal or bacterial infestation during storage.

The bactericidal composition is used for preventing and treating diseases of fruits and vegetables in the storage period.

The fungicidal compositions are particularly important for controlling a large number of fungi in various crop plants such as bananas, cotton, vegetable varieties (e.g., cucumbers, beans, tomatoes, and cucurbitaceae), barley, grasses, oats, coffee, potatoes, corn, fruit varieties, rice, rye, soybeans, grapevine, wheat, ornamentals, sugarcane, and a large number of seeds.

A method of controlling phytopathogenic fungi of plants, plant parts, plant propagation material and plant organs which grow at a later time, comprising applying the fungicidal composition of the invention in an agronomically effective and substantially non-phytotoxic application rate in the form of a seed treatment, foliar application, stem application, drench, drip, pour, spray, mist, dusting, spreading or smoking, to the plants, plant parts, plant propagation material or to the soil or to the cultivation medium in which the plants are growing or in which they are desired to grow.

The fungicidal composition of the present invention has a strong activity against various plant pathogenic bacteria, and can exert a strong control effect on the prevention and treatment of plant diseases caused by plant pathogenic bacteria. The fungicidal composition of the present invention has excellent activity against a wide range of phytopathogenic fungi such as Plasmodiophoromycetes, Oomycetes, Chytridiomycetes, Zygomycetes, Ascomycetes, Basidiomycetes, Deuteromycetes, Phycomycetes, etc.

The bactericidal composition also has very good bactericidal performance and can be used for preventing and controlling plant pathogenic bacteria. Such as pseudomonadaceae, rhizobiaceae, enterobacteriaceae, corynebacteriaceae and streptomycetaceae.

The fungicidal compositions of the invention can be used as foliar fungicides in crop protection, and also as fungicides for seed dressing and as soil fungicides.

The bactericidal composition is particularly suitable for controlling the following phytopathogens:

as the oomycetes, there may be exemplified, for example, Pythium species such as Rhizoctonia solani (Pythium ultium) of various crops; phytophthora species such as Phytophthora solani (Phytophthora infestans), Botrytis cinerea (Phytophthora capsici); pseudoperonospora species such as Pseudoperonospora cubeNsis (Pseudoperonospora cubeNsis), Pseudoperonospora humuli (Pseudoperonospora humuli); plasmopara species such as Plasmopara viticola; such as Peronospora brassiccus (Peronospora brassicae), Peronospora shallot (Peronospora destructor), Peronospora spinacia (Peronospora spiNacia), etc.

As Ascomycetes, there may be exemplified, for example, Erysiphe graminis (Erysiphegraminis) Erysiphe; sphaerotheca species such as vegetable powdery mildew (Sphaerotheca fuliginena); venturia bacteria such as Venturia iNaequalis (Venturia iNaequalis), Venturia piricola (Venturia nashicoloa); pyrenophora species such as barley Dictyophora (Pyrenophores); a bacterium belonging to the genus Cochliobolus such as barley speckled disease (Cochliobolus sativus); such as Sclerotinia sclerotiorum of vegetable Sclerotinia sclerotiorum (Sclerotinia sclerotiorum).

As the Basidiomycetes, there can be exemplified, for example, a fungus of the genus Bischospora such as PucciNiarecoNdita; tilletia species such as Tilletia foetida (Tilletia caries); such as Ustilago sp.of barley loose smut (Ustilago Nuda).

As the deuteromycotina, there can be exemplified, for example, a strain of the genus Phoma such as Phoma asparagi (Phoma asparagi); septoria such as sheath blight of wheat (Septoria Nodorum); colletotrichum such as Colletotrichum cucurbitacearum (Colletotrichum lageNarium); pyricularia species such as Fusarium oxysporum (Pyricularia oryzae); botrytis ciNerea such as Botrytis ciNerea; alternaria such as Alternaria alternata (Alternaria mali) and Alternaria solaNi (Alternaria solaNi); cercospora species such as Cercospora (Cercospora betacola); a species of the genus amycolata such as the species Cladosporium persicinum (Cladosporium carpophilum); such as Rhizoctonia species of Rhizoctonia solaNi (Rhizoctonia solaNi) belonging to the genus Rhizoctonia.

Crops treated with the fungicidal compositions of the present invention are, for example, but not limited to, bananas, cotton, vegetable varieties (e.g., cucumbers, beans, tomatoes, and cucurbitaceae), barley, grasses, oats, coffee, potatoes, corn, fruit varieties, rice, rye, soybeans, grapevines, wheat, ornamentals, sugarcane, and a wide variety of seeds.

Such crops include mainly field crops, such as corn, soybean, cotton, canola oil seeds, such as south Brassica napus (e.g. canola), turnip (Brassica rapa), mustard (b.juncea) (e.g. mustard (mustard)) and Brassica carinata, rice, wheat, sugar beet, sugar cane, oats, rye, barley, millet, triticale, flax, vines and fruit or vegetable crops of various plant classes, such as rosaceae (Rosaceaesp) (e.g. pome fruits, such as apples and pears, and also stone fruits, such as apricots, cherries, almonds and peaches, berries, such as strawberries, theaceae (ribeoiae sp.), jugaceae (judaiceae), Juglandaceae (Betulaceae), oleaceae (oleaceae), macadamia, fagus), fagochaetaceae (macaca), fagus (oleaceae), fagus canaceae (rosacee) and fagus canaceae (Brassica carinata) varieties) Lauraceae (Lauraceae sp.), Musaceae (Musaceae sp.) (e.g., banana trees and musa basjora (plantains)), Rubiaceae (Rubiaceae sp.) (e.g., coffee), theaceae (theaesp.), firmianaceae (sterculaceae sp.), Rutaceae (Rutaceae sp.) (e.g., lemon, orange, and grapefruit); solanaceae (solanaceae sp.) (e.g. tomatoes, potatoes, peppers, eggplants), Liliaceae (Liliaceae sp.), compositaceae (Compositiae sp.) (e.g. lettuce, artichoke and chicory-including root chicory (root chicory), endive (endive) or common chicory), Umbelliferae (Umbelliferae sp.) (e.g. carrots, parsley, celery and celeries), Cucurbitaceae (Cucurbitaceae sp.) (e.g. cucumbers-including pickled cucumbers (pickling cuumber), squash, watermelons, cucurbits and melons), Alliaceae (Alliaceae sp.) (e.g. onions and leeks), cruciferae.g. brassicae.g. white cabbage, red cabbage, broccoli, cabbage, cauliflower, brussels sprouts, cabbage, parsley, radish, leguminous, lentils such as lentils, beans, chenopodiaceae (Chenopodiaceae sp.) (e.g., fodder beet, spinach sweet (spinach beet), spinach, beetroot), Malvaceae (e.g., okra), asparagines (e.g., asparagus); horticultural and forest crops; an ornamental plant; and genetically modified homologues of these crops.

The fungicidal compositions of the present invention may also be used for the treatment of Genetically Modified Organisms (GMOs), such as plants or seeds. Genetically modified plants (or transgenic plants) are plants in which a heterologous gene has been stably integrated into the genome.

The term "heterologous gene" is essentially a gene that is provided or assembled outside the plant, when introduced into the nuclear, chloroplast or mitochondrial genome, to obtain a transformed plant with new or improved agronomic or other properties by expressing a protein or polypeptide of interest or by down-regulating or silencing other genes present in the plant (using, for example, antisense, co-suppression or RNA interference-RNAi techniques). Heterologous genes located in the genome are also referred to as transgenes. A transgene defined by a particular location in the plant genome is referred to as a transformation or transgenic event.

The fungicidal compositions of the present invention may also be used to treat fungal diseases that are susceptible to growth on or within wood. The term "wood" refers to all types of wood, and all types of processed materials such as solid wood, high density wood, laminated wood, and plywood, which are processed for the construction industry.

The fungicidal compositions according to the invention are particularly suitable for controlling the following phytopathogenic fungi:

erysiphe graminis (Erysiphe graminis) on cereals, Erysiphe graminis (Erysiphe graminearum) and Xanthium strumarium (Sphaerotheca fuliginea), Erysiphe graminis (Podosphaera leucotricha) on apples, Uncinula viticola (Uncinula necator) on grapes, Puccinia graminis (Puccinia) on cereals, Rhizoctonia solani (Rhizoctonia gossypii) on cotton, rice and lawn, Ustilago (Ustilago) on cereals and sugarcane, Venturia inaequalis (Venturia inaequalis) on apples, Helminthosporium graminis (Helminthosporium) on cereals, Rhizoctonia glumae (Sepilodorum) on wheat, strawberria, vegetables, Botrytis cinerea (Botrytisciana) on ornamental plants and grapes, Arachida (Ceriporia arachidicola) on peanuts (Ceriporia reticulata), Pseudocercospora graminis (Pseudoceria) on cucumbers (Pseudoceria graminis) on grapes, Pseudocerosa (Pseudoceria graminea) on grapes, Pseudocerosa (Pseudocerosa) on grapes, Pseudocerea fructicola (Pseudoceria and Pseudoceria graminea), Pseudocerosa (Pseudoceria serosa) on grapes, Pseudocerosa (Pseudocerosa and Pseudocerea) on grapes, Pseudocerosa (Pseudocerosa) on grapes, Pseudocerosa (Pseudocerosa, Popularia) on grapes, Pogostemrinia, Pogostemia, Alternaria on vegetables and fruits (Alternaria), Mycosphaerella on bananas (Mycosphaerella), and Fusarium (Fusarium) and Verticillium (Verticillium).

The fungicidal compositions according to the invention are particularly suitable for controlling downy mildew, early blight, late blight, black shank, scab, leaf spot, anthracnose, powdery mildew, wilting, scab, leaf rust on bananas, cotton, vegetable varieties (such as cucumbers, beans, tomatoes and cucurbitaceae), barley, grasses, oats, coffee, potatoes, maize, fruit varieties, rice, rye, soybeans, grapevine, wheat, ornamentals, sugarcane and a large number of seeds.

The invention provides a method for controlling plant pathogenic bacteria, which is characterized in that a bactericidal composition acts on the pathogenic bacteria and/or the environment thereof, or plants, plant parts, plant propagation materials, soil, materials or spaces.

The fungicidal compositions of the present invention are useful for the protection of plants, plant parts, plant propagation material and plant organs that grow at a later time.

The bactericidal composition can treat all plants and plant parts. "plant" means all plants and plant populations such as desirable and undesirable wild plants, cultivars, and plant varieties (whether or not protected by a plant variety or plant cultivar rights-to-human). Cultivated plants and plant varieties may be plants obtained by conventional propagation and cultivation methods, which may be supplemented or supplemented by one or more biotechnological methods, for example using dihaploids, protoplast fusion, random and directed mutations, molecular or genetic markers, or using bioengineering and genetic engineering methods. Plant parts are understood to mean all parts and organs of plants above and below the ground, such as shoots, leaves, flowers and roots, such as leaves, needles, stems, branches, flowers, fruit bodies, fruits and seeds, and also roots, bulbs and rhizomes. Also plants and vegetative and generative propagation material, for example cuttings, bulbs, rhizomes, runners and seeds, belong to the plant part.

The term "plant propagation material" is understood to mean all reproductively competent plant parts, such as seeds, which can be used for the propagation of the latter, and also vegetative materials, such as cuttings or tubers (e.g. potatoes). Thus, plant parts as used herein include plant propagation material. Mention may be made, for example, of seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated plants and useful plants to be inhibited after germination or after emergence from the soil. The young plants can be protected prior to transplantation by a total or partial treatment by dipping.

Preferred propagation material of the present invention is a seed. The fungicidal compositions of the present invention are also particularly suitable for the treatment of seeds. Most of the crop damage caused by harmful fungi is caused by the attack of the seeds during storage or after sowing and during or after germination of the plants. Plants are particularly sensitive to roots and shoots during the growing period and can cause death of the plant even if there is little damage. This stage is particularly critical, since it is very important to protect the seeds and the germinating plants by using suitable compositions.

The control of phytopathogenic fungi by treating the seeds of plants has been the subject of long-term research. Another aspect of the present invention provides a method for protecting seeds and germinating plants, which method makes it unnecessary or at least significant additional application of crop protection agents after sowing or after the emergence of the plants. On the other hand, the amount of active compound used is optimized with the fungicidal composition according to the invention in order to provide maximum protection of the seeds and the germinating plants from attack by phytopathogenic fungi, without the plants themselves being harmed by the active compound used.

The present invention therefore also relates in particular to a method for protecting seeds and germinating plants from attack by phytopathogenic fungi by treating the seeds with a composition according to the invention. The invention also relates to the use of the composition according to the invention for treating seeds to protect the seeds and germinating plants from phytopathogenic fungi. Furthermore, the present invention relates to seeds treated with the composition according to the invention for protecting against phytopathogenic fungi.

The control of phytopathogenic fungi which damage the post-emergent plants is effected primarily by treating the soil and the aerial parts of the plants with crop protection agents. In view of the possible effects of crop protection agents on the environment and on the health of humans and animals, it is therefore necessary to minimize the application rate of the active compounds.

The fungicidal compositions according to the invention are suitable for protecting the seed of any plant variety applied in agriculture, in greenhouses, in forestry or in horticulture-or grape cultivars. In particular, it takes the form of seeds of cereals (such as wheat, barley, rye, triticale, millet, oats), maize, cotton, soybeans, rice, potatoes, sunflowers, beans, coffee, sugar beet, peanuts, oilseed rape, olives, cocoa, sugar cane, tobacco, vegetables (such as tomatoes, cucumbers, onions and lettuce), turfgrass and decorative plants. The treatment of cereal and vegetable seeds is of vital importance.

The active ingredients of the bactericidal composition of the present invention, meptyldinocap and iprodione, are applied to seeds either alone or in a suitable formulation. It is preferably treated in a sufficiently stable state that the treatment does not cause any damage to the seed. In general, the treatment of the seeds can be carried out at any point in time between picking and sowing. The seeds commonly used are isolated from the plant and from the cob, husk, stem, cuticle, hair or pulp. Thus, for example, seeds that have been picked, cleaned and dried to a moisture content of less than 15% by weight may be used. Alternatively, seeds may be used which are dried, for example by treatment with water, and then dried again.

Examples of the method of seed treatment include a method of diluting a liquid or solid chemical, a method of directly immersing seeds in a liquid solution without dilution to allow the chemical to permeate the seeds, a method of mixing a solid chemical or liquid chemical with seeds to coat the seeds and thereby adhering the chemical to the surfaces of the seeds, and a method of spraying the chemical to the vicinity of the seeds while planting.

A plant part and plant organ that subsequently grows is any part of a plant produced from plant propagation material, such as seeds. Plant parts, plant organs and plants may also benefit from the pathogenic damage protection obtained by applying the fungicidal composition to plant propagation material. Certain plant parts and plant organs that grow after certain locations may also be considered plant propagation material, which itself may be applied (or treated) with the fungicidal composition; thus plants, other plant parts and other plant organs produced from the treated plant parts and treated plant organs may also benefit from the application of the germicidal composition.

The fungicidal compositions of the present invention may also be used to prevent or control a variety of pathogenic or saprophytic fungi and bacteria in soils or cultivation media. Examples of soil-borne fungal pathogens include Alternaria spp, Chaetomium spp, Botrytis cinerea, Cercospora spp, Claviceps purpurea, Cochliospora graminis (Cochliobolus sativus), Colletotrichum spp, Epicoccum spp, Fusarium graminearum (Fusarium graminearum), Fusarium oryzae (Fusarium moniliforme), Fusarium oxysporum (Fusarium oxysporum), Fusarium moniliforme (Fusarium moniliforme), Fusarium solani (Fusarium moniliforme), Fusarium venenatum (Fusarium moniliforme), Fusarium moniliforme (Fusarium septorium), Fusarium solani (Fusarium moniliforme), Fusarium oxysporum (Fusarium moniliforme), Fusarium solani (Fusarium moniliforme), Penicillium solanum (Rhizoctonium), Penicillium solanum (Rhizoctonia), Rhizoctonia solani (Rhizoctonia), Rhizoctoniensis), Rhizoctonia solani (Rhizoctonia solani), Rhizoctonia solani (Rhizoctoniensis), Rhizoctonia solani (Rhizoctonia solani), Rhizoctonia solani (Rhizoctonia), Rhizoctonia solani (Rhizoctoniensis), Rhizoctonia solani (Rhizoctonia solani), utilis species (Urosysticcculta), Ustilago spp or Verticillium spp.

The soil germs include rhizoctonia solani, fusarium, phytophthora, damping-off, root rot, pythium, botrytis cinerea, soft rot and the like. Under general conditions, soil pathogenic bacteria can generate a large amount of bacteria, as long as conditions are favorable for growth and development of the pathogenic bacteria and hosts are susceptible, the pathogenic bacteria can propagate in a large amount and infect the hosts, under the host infected with diseases, the pathogenic bacteria can enter a continuous pathogenic period, propagate and diffuse in a large amount along with continuous cropping of crops, but then nutrients are consumed, or when soil conditions such as temperature, humidity and the like are unfavorable for the pathogenic bacteria, the pathogenic bacteria can enter a dormant period. When the host with disease does not exist, soil-borne disease bacteria can survive in soil, and the soil-borne disease bacteria can survive on the root surface or the fallen leaves of the non-host except the soil-borne disease bacteria with wide host range and have the saprophytic competitive ability. However, different germs are different, and like fusarium can almost survive in soil indefinitely.

The culture medium of the present invention refers to a support capable of rooting and growing crops, such as: examples of the raw material include sand, pumice, vermiculite, diatomaceous earth, agar, gel, polymer, asbestos, wood chips, and bark.

Examples of methods for applying a chemical to soil include a method in which a liquid chemical is diluted in water or applied without dilution directly to the roots of a plant or a seedling bed for raising seedlings, a method in which granules are sown to the roots of a plant or a seedling bed for raising seedlings by spraying a powder, a water dispersible granule or the like to soil and mixing with the whole soil before sowing, and a method in which a powder, a water dispersible granule or the like is diluted and sprayed to a planting hole or a planting furrow before sowing or planting a plant, and sowing is performed.

The sterilization mixture can also be used for preventing and controlling diseases of fruits and vegetables in the storage period, and obtains an unexpected synergistic effect. For example fruit decay caused by the following pathogens:

aspergillus species, such as Aspergillus flavus;

botrytis (Botrytis) species, such as Botrytis cinerea (Botrytis cinerea);

penicillium (Penicillium) species, such as Penicillium expansum (Penicillium expansum) and p.purpurogenum;

sclerotinia (Sclerotinia) species, such as Sclerotinia (sclerotiorum);

verticillium species, for example Verticillium alboatrum.

The bactericidal composition is also particularly suitable for preventing and treating stalk rot, green mold, penicillium and anthracnose of fruits and vegetables in the storage period.

It is another object of the present invention to provide a method of controlling phytopathogenic fungi of plants, parts of plants, plant propagation material and plant organs which grow at a later time, which comprises applying the fungicidal composition of the present invention to the plants, parts of plants, plant propagation material or to the soil or to the cultivation medium in which the plants are growing or in which it is desired to grow, in an agronomically effective and substantially non-phytotoxic manner by seed treatment, foliar application, stem application, drench, drip, pour, spray, mist, dusting, scattering or smoking.

The germicidal compositions of the present invention may be applied by various treatment methods, such as:

-spraying a liquid comprising the fungicidal composition onto the above-ground parts of the plant;

-dusting, incorporating granules or powders in the soil, spraying around said plants and, in the case of tree injection or painting;

-coating or film coating the seeds of the plants.

When the preservative is used for preservation and fresh-keeping of picked fruits and vegetables, the preservative is usually diluted by water by 200 times and 2000 times, and the fruits are leached out after being soaked.

The present invention provides a method for controlling plant pathogenic bacteria, which can be a treatment, prevention or eradication method.

The fungicidal compositions according to the invention can also be applied during the growth of plants or plant parts in order to protect the harvested plants or plant parts.

According to the invention, post-harvest and storage-period diseases can be caused, for example, by the following fungi: colletotrichum species, such as banana Colletotrichum muscae (Colletotrichum musae), Colletotrichum disclinae (Colletotrichum gloeosporioides), capsicum Colletotrichum (Colletotrichum coccodes); fusarium species, such as Fusarium semitectum (Fusarium semitectum), Fusarium moniliforme (Fusarium moniliforme), Fusarium solani (Fusarium solani), Fusarium oxysporum (Fusarium oxysporum); verticillium species, such as, for example, Verticillium theobromae (Verticillium theobromae); a species of the genus Neurospora; botrytiscinella species, such as Botrytiscinella cinerea (Botrytiscinerea); geotrichum species, such as Geotrichum candidum (Geotrichum candidum); phomopsis species, Phomopsis natalensis (Phomopsis natalensis); species of the genus Lasiosphaera, such as, for example, Dichloropsis citrifolia (Diplodia citri); alternaria species, such as, for example, Alternaria citri (Alternaria citri), Alternaria alternata (Alternaria alternata); phytophthora species, such as Phytophthora citri (Phytophthora citrophthora), Phytophthora fragrans (Phytophthora fragaria), Phytophthora infestans (Phytophthora cactorum), Phytophthora nicotiana (Phytophthora parasitica), Septoria (Septoria spp.), such as Septoria depressa; mucor spp, such as Mucor piriformis (Mucorpiriformis); streptomyces (Monilinia spp.), such as, for example, Streptomyces fructicola (Monilinia fructicola), Streptomyces drupes (Monilinia laxa); venturia spp, such as Venturia inaequalis, Venturia pyrifera (Venturia inaegulis), Venturia pyrifolia (Venturia pyrina); rhizopus sp, such as Rhizopus stolonifer, Rhizopus oryzae (Rhizopus oryzae); genus Microtheca (Glomeellaspp.), e.g., pericarp (Glomeellacirata); sclerotinia spp, such as Sclerotinia fructicola (Sclerotinia fructicola); the genus longbeak (Ceratocystis spp.), such as the Kiwi long beak (Ceratocystis paradoxa); penicillium spp, such as Penicillium funiculosum (Penicillium funiculosum), Penicillium expansum (Penicillium expandasum), Penicillium digitatum (Penicillium digitatum), Penicillium italicum (Penicillium italicum); pediophora sp, e.g., Pediophora albuginea (Gloeosporium album), Gloeosporium perennans, Pediophora fructicola (Gloeosporium fructigenum), Gloeosporium singulata; the genus chitin of the genus chitin spora (Phlyctaenana spp.), such as Phlyctaenavagabunda; cylindrocarpon spp, such as, for example, Cylindrocarpon mali; stemphylium spp, such as stemphylium citrinum (stemphylium venelicanum); aschersonia (Phacydiopanis spp.), e.g., Phacydiopani malirum; rhizopus (Thielavissis pp.), for example, Rhizopus mirabilis (Thielavissis paradoxy); aspergillus spp, such as Aspergillus niger, Aspergillus carbonarius, Nectria sanguinalis, e.g. Nectria necator (Nectria gallinarum); amycolatopsis (Pezicula spp.).

The treatment method according to the invention can also be used to protect stored products from fungal and microbial attack. According to the invention, the term "stock" is understood to mean natural substances and processed forms thereof of plant or animal origin which have been derived from the natural life cycle and are intended to be preserved for a long period of time. Storage products of plant origin, for example plants or parts thereof, such as stems, leaves, tubers, seeds, fruits or grains, can be protected in the freshly harvested state or in processed form, such as (pre) drying, wetting, comminuting, grinding, pressing or baking. Or wood, in the form of coarse wood such as construction timber, utility poles and fences; or in finished form, such as furniture or articles made of wood. The animal-derived stock is hide, leather, hair, etc. The compositions according to the invention can prevent fungal or bacterial attack such as corrosion, discoloration or mildew on storage. "stock" is preferably understood as meaning natural substances of plant origin and processed forms thereof, more preferably fruits and processed forms thereof, such as pomes, stone fruits, stone-free small fruits and citrus fruits and processed forms thereof.

The fungicidal composition of the present invention can be used in the form of a conventional chemical preparation, for example, emulsifiable concentrate, wettable powder, suspension, liquid, granule, seed coating, and the like, and the application amount thereof varies depending on the mixing ratio of the active ingredients, weather conditions, the chemical preparation, the application time, the application method, the application site, the control of target pests, the target crop, and the like.

Treatment according to the invention may produce superadditive ("synergistic") effects. For example, depending on the application rate and/or broadening the activity spectrum and/or increasing the activity of the fungicidal compositions used according to the invention, it is possible to obtain the following effects: better plant growth, increased tolerance to high or low temperatures, increased tolerance to drought or to water or soil salt content, improved flowering performance, easier harvesting, accelerated ripening, higher harvest yields, larger fruits, higher plant height, greener leaf color, earlier flowering, higher quality or nutritional value of the harvested product, higher sugar concentration in the fruit, better storage stability and/or processability of the harvested product, which exceeds the actually predicted effects.

The treatment method of the invention may also be used to treat propagation material such as tubers or rhizomes, and may be used to treat seeds, seedlings or transplanted (packing out) seedlings and plants or transplanted plants. This processing method can also be used to process roots. The treatment method of the present invention can also be used for treating the above-ground parts of plants such as the stems, stems or stalks, leaves, flowers and fruits of the plants concerned.

Typically for leaf treatment: 0.1 to 10000g/ha, preferably 10 to 1000g/ha, more preferably 50 to 500 g/ha; for dipping or instillation administration, the dosage may even be reduced, particularly when an inert substrate such as asbestos or perlite is applied;

-for seed treatment: 2-5000g/100kg of seeds, preferably 3-1000g/100kg of seeds;

-applying a treatment to the soil or water surface: 0.1 to 10000g/ha, preferably 1 to 1000 g/ha.

For the preservation of picked fruits and vegetables, 200 times and 2000 times of liquid can be diluted, and the fruits can be drained after being soaked.

The above-mentioned dosages are only typical exemplary dosages, and the person skilled in the art will adjust the application rate in the actual application according to the actual circumstances and needs, in particular according to the nature of the plants or crops to be treated and the severity of the germs.

The meptyldinocap of the present invention is administered in combination/association with iprodione. Comprising separate, sequential or simultaneous application of meptyldinocap and iprodione. Preferably, the meptyldinocap and iprodione combination is in the form of a composition comprising meptyldinocap and iprodione.

The compositions of the present invention may be presented in a dosage form, i.e., the components of the composition are mixed, or the components of the composition may be provided in a single dose, mixed in a tank or container prior to use, and then diluted to the desired concentration. The preparation form provided by the invention is preferably the main form.

As a further improvement of the invention, the bactericidal composition of the invention can be formulated into any agriculturally acceptable dosage form.

As a further improvement, the dosage form of the bactericidal composition provided by the invention is a suspending agent, a seed coating agent, wettable powder, water dispersible granules, a microcapsule suspending agent, coated granules, extruded granules, missible oil, a microemulsion, an emulsion in water, an effervescent tablet, an ultra-low volume liquid and a suspoemulsion.

The bactericidal composition comprises meptyldinocap, iprodione, a filler and/or a surfactant.

The content of the meptyldinocap and the iprodione in the bactericidal composition is 5% -90%.

The content of the meptyldinocap and the iprodione in the bactericidal composition accounts for 10% -80% of the bactericidal composition.

The content of the meptyldinocap and the iprodione in the bactericidal composition is 20% -60% of the bactericidal composition.

According to the present invention, the term "filler" refers to a natural or synthetic organic or inorganic compound that can be combined or associated with an active compound to make it easier to apply to a subject (e.g. plants, crops or grasses). Thus, the filler is preferably inert, at least should be agriculturally acceptable. The filler may be solid or liquid.

The inactive medium that can be used in the present invention may be either solid or liquid, and examples of the solid medium material include: examples of the inorganic filler include vegetable powders (for example, particles of soybean powder, starch, cereal powder, wood powder, bark powder, saw dust, walnut shell powder, bran, cellulose powder, coconut shell, corn cob, and tobacco stalk, and residues after extraction of plant essence), paper, saw dust, synthetic polymers such as ground synthetic resins, clays (for example, kaolin, bentonite, and acid china clay), and talc powders. Silica (for example, diatomaceous earth, silica sand, mica, hydrous silicic acid, calcium silicate), activated carbon, natural minerals (for example, pumice, attapulgite, zeolite, etc.), calcined diatomaceous earth, sand, plastic media (for example, polyethylene, polypropylene, polyvinylidene chloride, etc.), inorganic mineral powders such as potassium chloride, calcium carbonate, calcium phosphate, etc., chemical fertilizers such as ammonium sulfate, ammonium phosphate, urea, ammonium chloride, etc., and soil fertilizers, and these may be used alone or in combination of 2 or more.

Examples of the liquid vehicle material that can be used include water, alcohols (e.g., methanol, ethanol, isopropanol, butanol, and ethylene glycol), ketones (e.g., acetone, methyl ethyl ketone, diisobutyl ketone, and cyclohexanone), ethers (e.g., diethyl ether, dioxane, methyl cellulose, and tetrahydrofuran), aliphatic hydrocarbons (e.g., kerosene, and mineral oil), aromatic hydrocarbons (e.g., benzene, toluene, xylene, mineral spirits, alkyl naphthalenes, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, and chlorobenzene), halogenated hydrocarbons, amides, sulfones, dimethyl sulfoxide, mineral and vegetable oils, and animal oils.

Examples of the surfactant that can be used for emulsifying, dispersing, and/or wetting the active ingredient compound include polyacrylic acid salts such as fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid ester, phosphoric acid ester of polyoxyethylene alcohol or phenol, fatty acid ester of polyhydric alcohol, alkaryl sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonate, branched polymer of high molecular comb, butyl naphthalene sulfonate, alkylaryl sulfonate, sodium alkylsulfosuccinate, oils and fats, condensation product of fatty alcohol and ethylene oxide, and alkyltaurate, and protein hydrolysates. Suitable oligosaccharides or polymers are based, for example, on ethylene monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with, for example, (poly) alcohols or (poly) amines.

For dispersion stabilization, attachment and/or binding of the active ingredient compounds, auxiliaries such as xanthan gum, magnesium aluminum silicate, gelatin, starch, cellulose methyl ether, polyvinyl alcohol, polyvinyl acetate and natural phospholipids (such as cephalin and lecithin) as well as synthetic phospholipids, bentonite, sodium lignosulfonate and the like can be used.

Wherein the antifreezing agent can be selected from ethylene glycol, propylene glycol, glycerol, and sorbitol. As the deflocculant for the suspendable product, an auxiliary such as a naphthalenesulfonic acid polymer, a polymeric phosphate, or the like can be used.

As the defoaming agent, a silicone defoaming agent can be used.

Colorants which may be used, for example, inorganic pigments such as iron oxide, titanium oxide and prussian blue; and organic pigments/dyes: alizarin dyes, azo dyes, and metal phthalocyanine dyes; and trace elements such as iron, manganese, boron, copper, cobalt, molybdenum and zinc salts.

Optionally, further additional components, such as protective colloids, binders, thickeners, thixotropic agents, penetrating agents, stabilizers, masking agents, can also be included.

The formulations of the invention can be prepared by mixing the active compounds with the customary additives in a known manner. Such as conventional extenders as well as solvents or diluents, emulsifiers, dispersants, and/or binders or fixatives, wetting agents, water repellents, if desired siccatives and colorants, stabilizers, pigments, defoamers, preservatives, thickeners, water and other processing aids.

These compositions include not only those which are immediately applicable to the subject to be treated by means of a suitable device, such as a spraying or dusting device, but also concentrated commercial compositions which are intended to be diluted prior to application to the subject.

The meptyldinocap-and iprodione-containing compositions of the present invention may also be administered in combination with other active ingredients, for example to broaden the spectrum of activity or to prevent the development of resistance. Such as fungicides, bactericides, attractants, insecticides, acaricides, nematicides, growth regulators, herbicides, safeners, fertilizers or semiochemicals and the like.

The active compounds meptyldinocap and iprodione can be applied simultaneously, or separately, or sequentially, the order of separate application usually having no effect on the results of the control.

The germicidal composition of the invention may be formulated primarily, i.e., the materials in the composition are already mixed, or the components of the composition may be provided in a single dose, mixed in a tank or tank prior to use, and then diluted to the desired concentration. The preparation form provided by the invention is preferably the main form.

Although the individual active compounds exhibit weak fungicidal activity, the combination has an activity which exceeds the simple sum of the activities. The synergistic effect of the fungicides is always present when the fungicidal activity of the active compound combination exceeds the sum of the activities of the active compounds when applied individually.

The fungicidal compositions of the invention have improved activity against harmful fungi at a reduced total amount of active compound applied (synergistic). The bactericidal composition of the present invention also has an excellent bactericidal effect against bacteria that exhibit resistance to existing bactericides.

According to the bactericidal composition, the meptyldinocap and iprodione are subjected to binary compounding, so that the obtained composition has a gain effect on the prevention and treatment effect, the bactericidal spectrum is expanded, the effect of one medicine for multiple purposes is achieved, and the drug resistance of germs is effectively slowed down or avoided. Surprisingly, the fungicidal activity of the fungicidal compositions according to the invention is significantly higher than the sum of the activities of the individual active compounds, there being an unpredictable, truly occurring synergistic effect, not merely a supplementation of the activities. The synergistic effect is particularly pronounced when the active compounds are present in the fungicidal compositions of the present invention in a particular weight ratio. However, the weight ratio of the active compounds in the fungicidal compositions according to the invention can vary within certain limits.

The invention provides a bactericidal composition which has higher activity and longer activity retention. The bactericidal composition has low dosage and low toxicity, and controls cereals, fruits, vegetables and seeds with fungal diseases.

The invention provides a bactericidal composition which has higher activity and longer activity retention.

The bactericidal composition provided by the invention has a synergistic effect and is suitable for preventing and treating harmful fungi. And the bactericidal composition is especially suitable for controlling powdery mildew fungi in cereals, vegetables, fruits, ornamental plants and grapevines.

Detailed Description

The invention will be further illustrated by the following specific formulation examples.

Formulation examples

Example 110% Nitrophenyl ester +40% Isomyclobutanil wettable powder

Nitrobenzoate 10%

Iprodione 40%

Sodium dodecyl sulfate 10%

Sodium lignosulfonate 5%

White carbon black 10%

Kaolin is complemented to 100%

Mixing the active ingredients, various auxiliary agents, fillers and the like according to the proportion of the formula, and crushing the mixture by an ultrafine crusher to obtain the wettable powder of 10 percent of meptyldinocap and 40 percent of iprodione.

Example 21% Nitrophenyl ester +20% Isomyclobutanil wettable powder

1 percent of nitre benzene bacteria ester

20 percent of iprodione

1 percent of calcium dodecyl benzene sulfonate

Sodium lignosulfonate 2%

Sucrose to make up to 100%

Mixing the active ingredients, various auxiliary agents, fillers and the like according to the proportion of the formula, and crushing the mixture by using a superfine crusher to obtain 1% of meptyldinocap and 20% of iprodione wettable powder.

Example 320% Nitrophenyl ester +5% Isomyclobutanil emulsifiable concentrate

Nitrobenzoate 20%

Iprodione 5%

Ethoxylated castor oil 5%

Calcium dodecyl benzene sulfonate 3%

SOLVESSO^TM200 to 100%

The components are prepared according to the proportion and are stirred uniformly to obtain a uniform phase.

EXAMPLE 45% Nitrophenyl ester +30% Isomyclobutanil wettable powder

5 percent of nitre benzene bacteria ester

30 percent of iprodione

Sodium dodecyl sulfate 10%

Sodium lignosulfonate 5%

White carbon black 10%

Kaolin is complemented to 100%

The components are mixed according to a proportion, ground and crushed to prepare the wettable powder.

Example 55% Nitrophenyl ester +50% Isomycrea Water dispersible granules

5 percent of nitre benzene bacteria ester

50 percent of iprodione

5 percent of modified calcium lignosulfonate

Sodium dodecyl sulfate 5%

5 percent of urea

Kaolin is complemented to 100%

Uniformly mixing the iprodione active ingredient, the dispersing agent, the wetting agent, the disintegrating agent and the filler according to the proportion of the formula, and crushing the mixture into wettable powder through air flow; then adding meptyldinocap and mixing uniformly; adding a certain amount of water, mixing and extruding to obtain the finished product. And drying and screening to obtain the 5% meptyldinocap and 50% iprodione water dispersible granule.

Example 65% Nitrophenyl ester +25% Isobaea Suspoemulsion

Oil phase:

5 percent of nitre benzene bacteria ester

Oleic acid methyl ester 10%

Ethoxylated castor oil 5%

Water phase:

iprodione 25%

Sodium salt of sulfonated naphthalene sulfonic acid-formaldehyde condensation product 1%

The water is complemented to 100 percent

Dissolving meptyldinocap in methyl oleate, and adding ethoxylated castor oil to obtain an oil phase; according to the formula, the iprodione, the sodium salt of the sulfonated naphthalene sulfonic acid-formaldehyde condensation product and water are ground and/or sheared at high speed to obtain the iprodione water suspension; the oil phase was added to the aqueous phase under stirring to obtain a suspoemulsion.

Example 710% Nitrophenyl ester +10% Isomycrea wettable powder

Nitrobenzoate 10%

Iprodione 10%

1 percent of sodium lignosulfonate

Sodium lauryl sulfate 2%

1% of highly dispersed silicic acid

Kaolin is complemented to 100%

Example 810% Nitrophenyl ester +80% Isobaea coated granules

Nitrobenzoate 10%

Iprodione 80%

3 percent of polyethylene glycol

1% of highly dispersed silicic acid

Calcium carbonate to make up to 100%

The finely ground active ingredient is uniformly coated onto the carrier moistened with polyethylene glycol in a mixer. In this way dust-free coated granules are obtained.

Example 920% Nitrophenyl ester +5% Isomyclobutanil emulsifiable concentrate

Nitrobenzoate 20%

Iprodione 5%

Ethoxylated castor oil 5%

Calcium dodecyl benzene sulfonate 3%

SOLVESSO^TM200 to 100%

Mixing the above components, and stirring to obtain transparent homogeneous phase.

Example 1020% Nitrophenyl ester +5% Isobaea microemulsion

Nitrobenzoate 20%

Iprodione 5%

Ethoxylated castor oil 15%

10 percent of calcium dodecyl benzene sulfonate

SOLVESSO^TM200 to 100%

Example 1120% Nitrophenyl ester +60% Isobaea extruded granules

Nitrobenzoate 20%

Iprodione 60%

Sodium lignosulfonate 4%

2 percent of carboxymethyl cellulose

Kaolin is complemented to 100%

The active ingredient is mixed with the auxiliaries and milled, the mixture being moistened with water. The mixture was extruded and then dried in an air stream.

Example 125% Nitrophenyl ester +10% Isomycrea suspension seed coating

5 percent of nitre benzene bacteria ester

Iprodione 10%

10 percent of fatty alcohol-polyoxyethylene ether disodium sulfosuccinate monoester

5 percent of modified calcium lignosulfonate

Xanthan gum 1%

1 percent of bentonite

Glycerol 5%

PVP-K30 1%

The water is complemented to 100 percent

The components are mixed according to a certain proportion and ground and/or sheared at a high speed to obtain the suspended seed coating agent.

Example 1310% Nitrophenyl ester +50% Isomycrea microcapsule suspension

ATLOX^TM4913 2%

0.05 percent of citric acid

0.1 percent of catalyst

10 percent of water

50 percent of iprodione

PAPI 1.35%

SOLVESSO^TM200 5%

ATLOX^TM4913 10%

0.3 percent of dispersant LFH

0.16 percent of defoaming agent

10 percent of urea

Nitrobenzoate 10%

The water is complemented to 100 percent

Mixing polymethylene polyphenyl polyisocyanate (PAPI), meptyldinocap, and Solvesso^TM200 into oil phase containing ATLOX^TM4913 in an aqueous solution, an emulsion is formed. Then heating and maintaining the temperature at 50 DEG^oAdding catalyst to react for 2 h. Cooling to obtain the microcapsule of the meptyldinocap.

ATLOX^TM4913 mixing dispersant LFH, defoamer, urea, iprodione and water in proportion, grinding and/or high-speed shearing to obtain uniform suspension.

And adding the obtained nitrophenyl bacteria ester microcapsule into a suspending agent of iprodione, and uniformly stirring to obtain a 10% nitrophenyl bacteria ester and 50% iprodione microcapsule suspending agent.

Example 145% Nitrophenyl ester +20% Isomyclobutanil Suspoemulsion

5 percent of nitre benzene bacteria ester

20 percent of iprodione

SOLVESSO^TM200 10%

Ethoxylated castor oil 4%

5 percent of disodium fatty alcohol polyoxyethylene ether sulfosuccinate monoester

5 percent of modified calcium lignosulfonate

Xanthan gum 1%

1 percent of bentonite

Glycerol 5%

The water is complemented to 100 percent

Dissolving meptyldinocap in SOLVESSO^TM200, adding ethoxylated castor oil to obtain an oil phase of the meptyldinocap;

mixing the fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoester disodium, the modified calcium lignosulfonate, the iprodione and water in proportion, and grinding and/or shearing at high speed to obtain the uniform suspension agent of the iprodione.

Adding the oil phase containing meptyldinocap into the water suspending agent containing iprodione to obtain the suspending emulsion.

Example 1525% Nitrophenyl ester +5% Isomyceliurea emulsifiable concentrate

Nitrobenzoate 25%

Iprodione 5%

Ethoxylated castor oil 5%

Calcium dodecyl benzene sulfonate 3%

SOLVESSO^TM200 to 100%

Example 165% Nitrophenyl ester +50% Isobaea Water dispersible granules

5 percent of nitre benzene bacteria ester

50 percent of iprodione

5 percent of modified calcium lignosulfonate

Sodium dodecyl sulfate 5%

5 percent of urea

Kaolin is complemented to 100%

Uniformly mixing meptyldinocap, iprodione, a dispersing agent, a wetting agent, a disintegrating agent and a filler according to the proportion of a formula, and performing air flow crushing to obtain wettable powder; adding a certain amount of water, mixing and extruding to obtain the finished product. And drying and screening to obtain the 5% meptyldinocap and 40% iprodione water dispersible granule.

Example 1710% Nitrophenyl ester +50% Isomycrea wettable powder

Nitrobenzoate 10%

50 percent of iprodione

Sodium dodecyl sulfate 1%

1 percent of sodium lignosulfonate

Kaolin is complemented to 100%

Example 1810% Nitrophenyl ester +90% Isomycrea

Nitrobenzoate 10%

Iprodione 90%

Mixing the meptyldinocap and iprodione uniformly according to the proportion.

Example 1930% Nitrophenyl ester +70% Isobaea

Nitrobenzoate 30%

Iprodione 70%

The proportion in the above examples is weight percent.

Biological test example:

the pesticide prepared by combining the effective components of different pesticides is an effective and quick way for developing and researching new pesticides and preventing and controlling resistant germs in agriculture at present. Pesticides of different species, when mixed, typically exhibit three types of action: additive action, synergistic action and antagonistic action. However, the specific action cannot be predicted, and can only be known through a large number of experiments. The compound synergist has a good formula, and the actual control effect is obviously improved, so that the use amount of the pesticide is reduced, and the generation of resistance is greatly delayed.

Firstly, toxicity test:

calculating the virulence index of each medicament and the cotoxicity coefficient (CTC value) of the mixture by a Sun Yunpei method, wherein when the CTC is less than or equal to 80, the composition shows antagonism, when the CTC is less than 80 and less than 120, the composition shows additivity, and when the CTC is more than or equal to 120, the composition shows synergism.

Observed virulence index (ATI) = (standard agent EC 50/test agent EC50) × 100

Theoretical virulence index (TTI) = a agent virulence index% percentage of a in a mixture + B agent virulence index% percentage of B in a mixture

Co-toxicity coefficient (CTC) = [ actually measured toxicity index (ATI)/Theoretical Toxicity Index (TTI) × 100 for mixed agent)

Plant pathogens and host plants for use in greenhouse virulence tests

Host plant	Disease name	Pathogenic organisms
			Wheat (Triticum aestivum L.)	Powdery mildew	Erysiphe graminis f.sptritici
Wheat (Triticum aestivum L.)	Leaf rust disease	Puccinia triticina (berk.) Kuntze
			Apple (Malus pumila)	Scab of sore	Venturia apple
Grape	Powdery mildew	Grape powdery mildew
			Cucumber (Cucumis sativus)	Powdery mildew	Erysiphe cichoracearum
Citrus fruit	Disease of penicillium	Penicillium
			Rice (Oryza sativa L.) with improved resistance to stress	Sheath blight disease	Rhizoctonia solani
Cucumber (Cucumis sativus)	Gray mold	Botrytis cinerea

Test 1: virulence determination of cucumber powdery mildew

Selected from consistent cucumber shoots, sprayed at 50PSI pressure using a potter spray tower, approximately 5mL per pot, with 12 concentration gradients set for each dose. Inoculating the cucumber powdery mildew leaves picked from the field 24 hours after the treatment of the agent, uniformly shaking off conidia above cucumber seedlings for inoculation, and then putting the cucumber seedlings into a greenhouse for cultivation. And 7d, surveying disease indexes according to disease classification standards of cucumber powdery mildew, calculating prevention and treatment effects, calculating the suppression median concentration EC50 by using a least square method, and calculating the cotoxicity coefficient (CTC) by using a Sun cloud Pepper method.

TABLE 1 virulence test results of the present invention on controlling cucumber powdery mildew

As can be seen from Table 1, when the weight ratio of the meptyldinocap to the iprodione is within the range of 50:1-1:50, the co-toxicity coefficients are both greater than 120, which indicates that the mixing ratio of the meptyldinocap to the iprodione within the range shows a gain effect.

Test 2: virulence determination of wheat leaf rust

Selected from wheat seedlings of uniform growth, each treatment selected 3 pots of test leaf seedlings, sprayed with a potter spray tower at 50PSI pressure, approximately 5mL per pot, each dose set with 12 concentration gradients. Inoculating the strain 24h after the treatment of the agent, uniformly shaking off conidia on the wheat seedlings from the field-picked wheat leaf rust disease leaves, inoculating, and then putting the wheat seedlings into a greenhouse for culturing. And 7d, surveying disease indexes of the whole plants according to the disease grading standard of the wheat leaf rust, calculating the prevention and treatment effect, calculating the inhibition medium concentration EC50 by using a least square method, and calculating the cotoxicity coefficient (CTC) by using a Sun cloud Pepper method.

Table 2: toxicity test result of the invention on preventing and treating wheat leaf rust

As can be seen from Table 2, when the weight ratio of the meptyldinocap to the iprodione is within the range of 50:1-1:50, the co-toxicity coefficients are both greater than 120, which indicates that the synergistic effect is shown by the blending of the meptyldinocap and the iprodione within the range.

Test 3: virulence determination of grape powdery mildew

Six week old grape plants were selected that retained two intact open leaves. Spray with a potter spray tower at 50PSI pressure, approximately 5mL per basin, with 12 concentration gradients set for each dose. Inoculating the strain 24h after the treatment of the agent, uniformly shaking off conidia on the grape plants to inoculate the diseased leaves infected with the grape powdery mildew, and then putting the grape plants into a greenhouse for culturing. And 7d, surveying disease indexes according to the disease classification standard of grape powdery mildew, calculating the prevention and treatment effect, calculating the suppression median concentration EC50 by using a least square method, and calculating the cotoxicity coefficient (CTC) by using a Sun cloud Pepper method.

TABLE 3 virulence test results of the present invention on prevention of grape powdery mildew

As can be seen from Table 3, when the weight ratio of meptyldinocap to iprodione is in the range of 50:1-1:50, the co-toxicity coefficients are both greater than 120, which indicates that the compounding of meptyldinocap and iprodione in the range shows a gain effect.

Test 4: virulence determination of wheat powdery mildew

Selected from uniformly growing wheat seedlings, sprayed with a potter spray tower at 50PSI pressure, approximately 5mL per pot, and set up 12 concentration gradients per dose. Inoculating the strain 24h after the treatment of the agent, uniformly shaking off conidia above wheat seedlings from wheat powdery mildew leaves picked in the field, and then putting the wheat seedlings into a greenhouse for culturing. And 7d, surveying disease indexes according to the incidence grading standard of the wheat powdery mildew, calculating the prevention and treatment effect, calculating the inhibition medium concentration EC50 by using a least square method, and calculating the cotoxicity coefficient (CTC) by using a Sun cloud Pepper method.

TABLE 4 virulence test results of the present invention on prevention and treatment of wheat powdery mildew

As can be seen from Table 4, when the weight ratio of meptyldinocap to iprodione is in the range of 50:1-1:50, the co-toxicity coefficient is greater than 120, which indicates that the compounding ratio of meptyldinocap to iprodione in the range shows a gain effect.

Test 5: toxicity assay for apple scab

Apple seedlings were selected and sprayed with a potter spray tower at 50PSI pressure, approximately 5mL per pot, with 12 concentration gradients set for each dose. Inoculating the strain 24h after the treatment of the agent, uniformly shaking off conidia on apple seedlings from diseased leaves infected by apple scab for inoculation, and then putting the apple seedlings into a greenhouse for culture. And 7d, surveying disease indexes of the whole plants according to the incidence grading standard of apple scab, calculating the prevention and treatment effect, calculating the inhibition medium concentration EC50 by using a least square method, and calculating the cotoxicity coefficient (CTC) by using a Sun Yunpei method.

TABLE 5 virulence test results of the present invention in the prevention and treatment of apple scab

As can be seen from Table 5, when the weight ratio of meptyldinocap to iprodione is in the range of 50:1 to 1:50, the cotoxicity coefficient is greater than 120, which indicates that the compounding ratio of meptyldinocap to iprodione in the range shows a gain effect.

Test 6: virulence determination of rice sheath blight

Selected from uniformly grown rice seedlings, sprayed with a potter spray tower at 50PSI pressure, approximately 5mL per pot, with 12 concentration gradients set for each dose. Inoculating the bacterial strain 24h after the treatment of the chemical, uniformly shaking off conidia above rice seedlings from rice sheath blight leaves picked in the field for inoculation, and then putting the rice seedlings into a greenhouse for cultivation. And 7d, surveying disease indexes of the whole plants according to the disease grading standard of the rice sheath blight, calculating the prevention and treatment effect, calculating the inhibition medium concentration EC50 by using a least square method, and calculating the cotoxicity coefficient (CTC) by using a Sun Yunpei method.

TABLE 6 virulence test results of the present invention in the control of rice sheath blight disease

As can be seen from Table 6, when the weight ratio of meptyldinocap to iprodione is in the range of 50:1-1:50, the co-toxicity coefficients are both greater than 120, which indicates that the compounding of meptyldinocap and iprodione in the range shows a gain effect.

Test 7: toxicity assay for penicilliosis in citrus

Adopting a method for inhibiting the growth rate of hypha: the test target is citrus penicilliosis.

Respectively dissolving meptyldinocap and iprodione in acetone, diluting with 0.1% tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into a sterilized triangular flask in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and preparing into 4 toxic culture media with corresponding concentrations; the toxic culture medium is prepared by compounding liquid medicines of meptyldinocap and iprodione at different ratios and series concentrations by the same method. The penicillium citrinum cultured for 2 days is beaten into bacterium blocks at the edges of the bacterial colonies by a puncher with the diameter of 5mm, the bacterium blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterium blocks are cultured in an incubator at 25 ℃, and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

then calculating the concentration EC in the inhibition by using a least square method₅₀Then, the cotoxicity coefficient (CTC) was calculated by the Sun Yunpei method.

Table 7: toxicity test results for citrus penicilliosis

From table 7, it can be seen that when the combination of meptyldinocap and iprodione is used for preventing and treating penicilliosis of citrus, the cotoxicity coefficients are both greater than 120 in the range of the mixture ratio of 50:1 to 1:50, which indicates that the blending of the meptyldinocap and the iprodione in the range shows a gain effect.

Test 8: virulence determination of cucumber gray mold

Selected from consistent cucumber shoots, sprayed at 50PSI pressure using a potter spray tower, approximately 5mL per pot, with 12 concentration gradients set for each dose. Inoculating the cucumber botrytis leaves collected from the field 24 hours after the treatment of the agent, uniformly shaking off conidia above cucumber seedlings for inoculation, and then putting the cucumber seedlings into a greenhouse for cultivation. And 7d, surveying disease index according to disease grading standard of cucumber gray mold, calculating prevention and treatment effects, calculating the inhibitory median concentration EC50 by using a least square method, and calculating the cotoxicity coefficient (CTC) by using a Sun cloud Pepper method.

TABLE 8 virulence test results of the present invention on prevention and treatment of cucumber gray mold

As can be seen from Table 8, when the weight ratio of meptyldinocap to iprodione is in the range of 50:1-1:50, the co-toxicity coefficient is greater than 120, which indicates that the mixing ratio of meptyldinocap to iprodione in the range shows a gain effect.

Claims

1. A germicidal composition, characterized by: the bactericidal composition contains active ingredients of meptyldinocap and iprodione, and the weight ratio of the meptyldinocap to the iprodione is 50:1-1: 50.

2. The bactericidal composition of claim 1, wherein the weight ratio of meptyldinocap to iprodione is from 25:1 to 1: 25.

3. The bactericidal composition of claim 1, wherein the weight ratio of meptyldinocap to iprodione is from 10:1 to 1: 10.

4. The bactericidal composition of claim 1, wherein the weight ratio of meptyldinocap to iprodione is from 5:1 to 1: 5.

5. The germicidal composition of claim 1, wherein: the sum of the mass of the meptyldinocap and the mass of the iprodione accounts for 5-90% of the mass of the bactericidal composition.

6. The germicidal composition of claim 1, wherein: the sum of the mass of the meptyldinocap and the mass of the iprodione accounts for 10-80% of the mass of the bactericidal composition.

7. The germicidal composition of claim 1, wherein: the sum of the mass of the meptyldinocap and the mass of the iprodione accounts for 20-60% of the mass of the bactericidal composition.

8. The germicidal composition of claim 1, wherein: the dosage form of the bactericidal composition is suspending agent, seed coating agent, wettable powder, water dispersible granules, microcapsule suspending agent, coated granules, extruded granules, missible oil, microemulsion, emulsion in water, effervescent tablets, ultra-low volume liquid and suspoemulsion.

9. The germicidal composition of claim 1, wherein: also comprises a filler and/or a surfactant.

10. A method of controlling phytopathogenic fungi, characterized by: the method of treating a pathogen and/or its environment, or a plant, seed, soil, material or space with the germicidal composition of claim 1.

11. The method of claim 10, wherein: simultaneously, separately or sequentially administering meptyldinocap and iprodione according to claim 1.

12. Use of the fungicidal composition of claim 1 for the control of fungi and bacteria.

13. Use of the fungicidal composition according to claim 1 for the control of fungi and bacteria on cereals, fruits, ornamentals and grapevines.

14. Use of the fungicidal composition according to claim 1 for the protection of plants, plant parts, plant propagation material and plant organs that grow at a later time.

15. Use of the fungicidal composition of claim 1 for controlling pathogenic or saprophytic fungi and bacteria in soil or cultivation media, applied to the locus where control is desired.

16. Use of the fungicidal composition of claim 1 for the protection of post-harvest plants or plant parts.

17. Use of the fungicidal composition of claim 1 for protecting stored products from fungal or bacterial infestation during storage.

18. A method of controlling phytopathogenic fungi of plants, plant parts, plant propagation material and plant organs which grow subsequently, characterized in that: the method comprises applying the fungicidal composition of claim 1 in an agronomically effective and substantially non-phytotoxic application rate to a plant, plant part, plant propagation material, or soil or cultivation medium in which the plant is growing or in which it is desired to grow, in a manner such as seed treatment, foliar application, stem application, drench, drip, pour, spray, dusting, scattering, or fuming.