CN108719315A

CN108719315A - A kind of bactericidal composition

Info

Publication number: CN108719315A
Application number: CN201710262961.3A
Authority: CN
Inventors: 罗昌炎; 詹姆斯.T.布里斯托
Original assignee: Jiangsu Rotam Chemical Co Ltd
Current assignee: Jiangsu Rotam Chemical Co Ltd
Priority date: 2017-04-20
Filing date: 2017-04-20
Publication date: 2018-11-02

Abstract

The present invention relates to a kind of bactericidal compositions；The invention further relates to the methods for using the bactericidal composition to prevent plant pathogen.The present invention provides a kind of bactericidal composition, the bactericidal composition active constituent is formed by gram bacterium is strong with zinc thiazole, and the strong weight ratio with zinc thiazole of gram bacterium is 20:1-1:20.The present invention also provides a kind of methods of prevention plant pathogen, the method includes acting on bactericidal composition of the present invention in plant pathogen and/or its environment or plant, plant part, plant propagation material and the plant organ then grown, soil or cultivation medium, material or space.The present invention also provides purposes of the bactericidal composition in improving plant health.

Description

A kind of bactericidal composition

Technical field

The present invention relates to a kind of bactericidal compositions, further relate to a kind of method of prevention plant pathogen.

Background technology

In recent years, the plant disease caused by various plant pathogenic fungis and bacterium was on the rise, from 80 years last century Dai Qi, pesticide company of various countries develop a variety of efficient chemical fungicide successively, such as triazole type, strobilurin fungicide, Substantially increase the control effect of plant disease.But the lasting use of chemical pesticide accelerates the drug resistance of disease, and remains Height pollutes the environment.

Biological pesticide refers to utilizing biological living (fungi, bacterium, insect viruses, genetically modified organism, natural enemy etc.) or its generation It thanks to product (pheromones, auxin, methyl α-naphthyl acetate, 2,4-D etc.) and is directed to the preparation that agricultural pest is killed or inhibited.Mainly Including plant insecticide, botanical fungicide, botanical herbicides and the mould poison of plant photoactivation etc..Biological pesticide has low Poison, low-residual the advantages of, and can it is simultaneous control numerous plant pathogenic fungis and bacterial disease, be widely used in it is organic and The prevention of the disease of green agricultural product.But most biological pesticides all have that drug effect is slow, the not ideal enough disadvantage of preventive effect, seriously It influences the application of these medicines and promotes.

About pesticide activity, especially to crop protection, one of the key problem of research carried out in the technical field is Improve the performance in terms of performance, especially bioactivity and the performance in terms of keeping this activity within a certain period of time.

Zinc thiazole, its chemical name is：It is double（2- amino -5- sulfydryl -1,3,4- thiadiazoles）Zinc, molecular structural formula are：

The structure of zinc thiazole forms sterilization by two groups.First, thiazolyl group, no inhibition on bacteria outside the plant, but It is efficient therapeutic agent in plant, in the plant's holey catheter, bacterium is severely damaged for medicament, and cell wall is thinning Then disintegrate, lead to the death of bacterium；Second is that zinc ion, has the function of not only antifungal but also bactericidal.Zinc ion in medicament It is exchanged with the cation (H+, K+ etc.) on pathogen cell membrane surface, the protein coagulating on germ cell film is caused to kill disease Bacterium；It is intracellular that part zinc ion penetrates into pathogen, is combined with certain enzymes, its activity is influenced, and leads to dysfunction, germ because And failure is dead.Under the collective effect of two groups, kill that germ is more thorough, and control effect is more preferable, controlling object is more extensive.Thiophene Azoles zinc can effectively prevent the bacterial diseases such as bacterial blight of rice, bacterial leaf streak of rice and citrus bacterial canker disease.

Gram bacterium is strong, also known as O, O- diethyldithioposphoric acid ammoniums（O,O-diethyl phosphordithiooic acid ammonium）.Gram bacterium is strong to belong to protective fungicide, is mainly used for preventing bacterial blight of rice, bacterial stripe, simultaneous rice of harnessing the river Banded sclerotial blight.Also there is preferable preventive effect to citrus ulcer.There are stimulating growth and production-increasing function to wheat, rice, vegetables etc..

Invention content

It is an object of the present invention to provide a kind of bactericidal composition, there can be greater activity and activity keeps more long sterilization Composition.

It provides that there is phytopathogen in the case where the application total amount of reactive compound reduces and improves active composition （Synergistic composition）, to reduce the rate of application of known activity compound and improve its activity profile.

And, it has been found that meanwhile, i.e., jointly or separately application gram bacterium is strong and zinc thiazole, or successively application gram bacterium it is strong and Zinc thiazole preferably to prevent plant pathogenic fungi than each compound is administered alone.

The present invention provides a kind of bactericidal composition, the composition carries out binary built by strengthening gram bacterium with zinc thiazole, So that obtained mixture has gain effect in control effect, and fungicidal spectrum is expanded, has effectively slowed down or avoid germ It develops immunity to drugs.Astoundingly, the bactericidal activity of bactericidal composition of the invention adds than the active of each reactive compound With it is considerably higher；There are unpredictable, necessary being synergistic effects, rather than just active supplement.

When reactive compound is present in specific weight ratio in the bactericidal composition of the present invention, synergistic effect is especially bright It is aobvious.But the weight ratio of the reactive compound in bactericidal composition of the present invention can change in a certain range.

A kind of bactericidal composition of the present invention is that following technical scheme is taken to realize：

The present invention provides a kind of bactericidal composition, and active constituent is formed by gram bacterium is strong with zinc thiazole, wherein gram bacterium is strengthened and zinc thiazole Weight percent be 20:1-1:20, preferably 15:1-1:15,10:1-1:10, further preferably 5:1-1:5.

Gram bacterium is strong and zinc thiazole that synergistic effect is obtained in the ratio range is particularly evident.

The strong weight proportion with zinc thiazole of gram bacterium in the present invention for example can also be 20:1,19:1,18:1,17:1,16: 1,15:1,14:1,13:1,12:1,11:1,10:1,9:1,8:1,7:1,6:1,5:Isosorbide-5-Nitrae:1,3:1,2:1,1:1,1:2,1:3, 1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,1:15,1:16,1:17,1:18,1:19,1: 20。

In the bactericidal composition, the strong 5%- that the Bactericidal composite amount of substance is accounted for zinc thiazole quality sum of gram bacterium 90%, more preferable 10%-80%, more preferable 20%-60%.

In the bactericidal composition of the present invention, gram bacterium is strong and the quality of zinc thiazole account for the bactericidal composition by weight also 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21% is may, for example, be, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 65%, 70%, 75%, 80%, 85%, 90%.

A method of the bactericidal composition is acted on plant pathogen and/or its ring by prevention plant pathogen Border or plant, plant part, plant propagation material and the plant organ then grown, soil or cultivation medium, material or sky Between in.

A method of the bactericidal composition is acted on plant or plant part by prevention plant pathogen.

A method of the bactericidal composition is acted on plant propagation material and then length by prevention plant pathogen The plant organ gone out.

A method of the bactericidal composition is acted on soil or cultivation medium by prevention plant pathogen.

A method of gram bacterium is strengthened and is administered simultaneously with zinc thiazole or applies respectively or apply in succession by prevention plant pathogen With.

A method of prevention plant pathogen, can plant by before infecting or after infecting by the Bactericidal composite Object acts on plant pathogen and/or its environment or plant, plant part, plant propagation material and the plant then grown In organ, soil or cultivation medium, material or space.

A method of prevention plant pathogen includes with agronomy by the bactericidal composition of the present invention effectively and substantially without plant The amount of application of object toxicity applied with seed treatment, foliage applying, stem, is impregnated with, instiled, poured into a mould, sprayed, sprayed, dusting, distribution or The methods of smoke is applied to plant pathogen and/or its environment or plant, plant part, plant propagation material and subsequent length In the plant organ, soil or cultivation medium, material or the space that go out.

The bactericidal composition is for preventing Cereal, veterinary antibiotics, fungi and bacterium on ornamental plant and grapevine Purposes.

Purposes of the bactericidal composition for protecting plant, plant propagation material and the plant organ then grown.

The bactericidal composition is applied to pathogenic or saprophytic in the required place prevention soil prevented or cultivation medium The purposes of fungi and bacterium.

The bactericidal composition is used to handle seed the purposes invaded with the phytopathogen for protecting seed to carry.

The bactericidal composition is used to protect the purposes of the fruits and vegetables after harvest.

The bactericidal composition is for protecting stock in storage period from fungi or the purposes of bacterial invasion.

Purposes of the bactericidal composition in improving plant health.

The bactericidal composition is used to increase the purposes of crop leaf chlorophyll content.

The bactericidal composition is used to increase the purposes of crop yield.

A kind of bactericidal composition, including gram bacterium is strong and zinc thiazole and filler and/or surfactant.

A kind of bactericidal composition can be configured to the arbitrary dosage form agriculturally allowed.The bactericidal composition, dosage form are Suspending agent, seed coat agent, suspension emulsion, wettable powder, water dispersible granules, microcapsule suspending agent, coated granule, squeeze out granule, Missible oil, microemulsion, aqueous emulsion, effervescent tablet, ultra low volume liquids.

The bactericidal composition can be applied without dilution, and application can also be diluted with water.

The bactericidal composition of the present invention has very strong activity to various phytopathogens, and can be to being drawn by phytopathogen The prevention and treatment of the plant disease risen play very strong preventive effect.

Bactericidal composition of the present invention can be in crop protection for preventing Ascomycetes（Ascomycetes）, load Daughter bacteria guiding principle（Basidiomycetes）, Phycomycetes（Phycomycetes）And deuteromycetes（Deuteromycetes）Fungi.

Bactericidal composition of the present invention can be in crop protection for preventing pseudomonad （Psedomonadaceae）, rhizobium (Rhizobiaceae), enterobacteria (Enterbacteriaceae), bar bacterium (Cornebacteriaceae) and streptomycete (Streptomycetaceae).

As example but unrestricted mode proposes is included into some of above-mentioned major class and leads to fungal disease and bacteriosis Pathogen：

Flavobacterium strain, such as rice bacterial leaf spot Xanthomonas campestris (Xanthomonascampestrispv.oryzae)；

Pseudomonas strain, such as Bacterial leaf spot cucumber pvs oryzae and oryzicola (Pseudomonas syringaepv.Lachrymans) ；

Erwinia strain, such as pears water epidemic disease Irving (family name) bacterium (Erwiniaamylovora)；

Rotten mould (Pythium) strain, such as Pythium ultimum (Pythium ultimum)；

Phytophthora (Phytophthora) strain, such as phytophthora infestans (Phytophthorainfestans)；

False downy mildew (Pseudoperonospora) strain, such as grass false downy mildew (Pseudoperonospora humuli) or ancient Bar false downy mildew (Pseudoperonosporacubensis)；

Axis downy mildew (Plasmopara) strain, such as grape life axis downy mildew (Plasmoparaviticola)；

Disk downy mildew (Bremia) strain, such as lettuce disk downy mildew (Bremia lactucae)；

Downy mildew (Peronospora) strain, such as pea downy mildew (Peronospora pisi) or Cruciferae downy mildew (P.brassicae)；

Powdery mildew (Erysiphe) strain, such as Erysiphe graminis (Erysiphe graminis)；

Sphaerotheca (Sphaerotheca) strain, such as balsamine list softgel shell (Sphaerothecafuliginea)；

Podosphaera (Podosphaera) strain, such as white cross hair list softgel shell (Podosphaeraleucotricha)；

Venturia (Venturia) strain, such as apple black star bacteria (Venturiainaequalis)；

Pyrenophora (Pyrenophora) strain, such as round nuclear cavity bacteria (Pyrenophorateres) or wheat nuclear cavity bacteria (P.graminea) (conidial form：Drechslera, Syn：Helminthosporium) ；

Cochliobolus category (Cochliobolus) strain, such as standing grain cochliobolus (Cochliobolussativus) (mitogenetic spore Sub- form：Drechslera, Syn：Helminthosporium) ；

Uromyces (Uromyces) strain, such as wart top uromyce (Uromycesappendiculatus)；

Handle rest fungus (Puccinia) strain, such as Puccinia recondita (Puccinia recondita)；

Sclerotinia (Sclerotinia) strain, such as Sclerotinia sclerotiorum (Sclerotiniasclerotiorum)；

Tilletia (Tilletia) strain, such as Tilletia caries (Tilletiacaries)；

Smut (Ustilago) strain, such as naked smut (Ustilago nuda) or oat smut (Ustilago avenae) ；

Film lead fungi category (Pellicularia) strain, such as the wooden film lead fungi (Pelliculariasasakii) of assistant assistant；Pears Spore (Pyricularia) strain, such as Magnaporthe grisea (Pyricularia oryzae)；

Fusarium (Fusarium) strain, such as yellow fusarium (Fusarium culmorum)；

Botrytis (Botrytis) strain, such as Botrytis cinerea (Botrytis cinerea)；

Septoria (Septoria) strain, such as phaeosphaeria nodorum (Septoria nodorum)；

Leptosphaeria (Leptosphaeria) strain, such as Leptosphaeria nodorum；

Cercospora (Cercospora) strain, such as graying tail spore (Cercosporacanescens)；

Alternaria (Alternaria) strain, such as rape rod method (Alternariabrassicae)；

False Cercosporalla (Pseudocercosporella) strain, such as wheat Phyllostachys pubescens (Pseudocercosporella herpotrichoides) ；

Phakopsora (Phakopsora) strain, such as Phakopsora pachyrhizi (Phakopsorapachyrhizi) and mountain horseleech layer Rest fungus (Phakopsora meibomiae)；And

Rhizoctonia (Rhizoctonia) strain, such as Rhizoctonia solani Kuhn (Rhizoctoniasolani).

The crop plants that the bactericidal composition of the present invention is suitable include mainly：Cereal crops, for example, wheat, barley, oat, Naked barley, triticale, rice, corn, sorghum and millet；Vine crop, such as Table Grape and vinifera；Field crop, example Such as rape（Canola）, sunflower；Sugar beet, sugarcane, soybean, peanut（Peanut）, tobacco, clover, clover, Hu Zhi Son, clover and vetch；Pip fruit, such as apple, pears, crabapple, loquat, hawthorn and quince；Stone fruit, example Such as peach, cherry, plum, apricot, honey peach；Citrus fruit, such as lemon, bitter orange, orange, shaddock, mandarin orange（Orange）And kumquat； Radicant and field crop（And their leaf）, such as arithoke, common beet and sugar beet, carrot, cassava, life Ginger, ginseng, horseradish, parsnip, potato, little radish, turnip, sweet potato, turnip and Chinese yam；Bulb bearing plant, such as Garlic, leek, onion and verdant；Leaf vegetables plant, such as mustard seed bitter dish（Rocket salad）, celery, celery, Chinese celery, witloof（Thatch dish）, Fennel, bile ingredients and leaf lettuce, parsley, red witloof（Red witloof）, rheum officinale, spinach and Swiss chard；Btassica（Koryo Dish）Leaf vegetables, such as broccoli, cauliflower（Broccoli）, it is Brussels sprouts, cabbage, Chinese cabbage, cauliflower, wild cabbage, collard, big Head dish, leaf mustard and green vegetables；Bean（It is succulence or without juice）Such as lupin, Kidney bean）（Including broad bean, kidney bean, dish Beans, Hua Dou, scarlet runner bean, snap beans, wide leaf vegetables beans and wax bean）, Kidney bean（Vigna belongs to）(including red bean, long cowpea Beans, cow gram, black cowpea, fresh kidney beans, cowpea, mung bean, cowpea, urd bean and speciality cowpea）, broad bean, chick-pea, melon ear, sword bean, soldier Beans and pea（Pisum belongs to）(including kidney bean, edible podded pea, field pea, pea, green pea, snow bean, sweet tea beans, pigeonpea and Soybean）；Fruit vegetables, such as eggplant）, Solanum muicatum and capsicum（Including bell pepper, capsicum, capsicum for cooking, pimiento, pimento；Small kind Eggplant and tomato）；Cucurbit class vegetables, such as chocho（Fruit）, wax gourd, citron watermelon, cucumber, young cucumber, marrow squash（Packet Include cucurbit, lagenaria sicerariae）, sponge gourd, gumbo, balsam apple, dioecism momordica root, balsam pear and Chinese cucumber, muskmelon, cucurbita pepo and winter squash and watermelon； Berries, such as blackberry, blueberry, the red fruit certain kind of berries, the dew certain kind of berries, the purplish blue certain kind of berries, blueberry, Cranberry, currant, the wild certain kind of berries, Loganberry, raspberry and strawberry； The raw nut of tree, such as almond, beech nut, Brazil nut, butternut, cashew nut, Chinese chestnut, fibert（Filbert）, hickory nut, Australia it is hard Fruit, pecan and English walnut；Tropical fruit (tree) and other crops, such as banana, plantain, mango, coconut, pawpaw, avocado, litchi Branch, American aloe, coffee, cocoa, sugarcane, oil palm, sesame, rubber and fragrance；Fibre crops, such as cotton, flax and hemp；Grass Leather and fur（Including season type and shitivi type sod grass）.

The bactericidal composition of the present invention can be used for therapeutic or protective control plant pathogen.Therefore, the present invention also relates to And the therapeutic and protectiveness method of plant pathogen is prevented using the bactericidal composition of the present invention, by the present invention's in this method Bactericidal composition is applied to the soil of seed, plant or plant part, fruit or plant growth.

The present invention bactericidal composition be especially suitable for prevention mandarin tree, peach, Chinese cabbage, tomato, cucumber, capsicum, potato, It is grape, balsam pear, lettuce, tobacco, turfgrass, rice, peanut, mandarin tree, wheat, barley, oat, true on seeding corn and other crops plant Bacterium and bacterium.

The bactericidal composition of the present invention is especially suitable for prevention citrus scab, resin disease, burst tuberculosis, foot rot；Rice is white Leaf blight, bacterial stripe, rice blast, banded sclerotial blight；Target, late blight；Brassicaceous vegetable black spot, black corruption Disease；Carrot leaf pinta；Celery bacterial speck, early epidemic scrofula, spot blight；Eggplant bacterial wilt, early blight, anthracnose, foxiness Disease；Common bacterial blight of bean；Green onion class purple spot, downy mildew；Pepper bacterial leaf spot；Cucumber bacterial angular leaf spot；Muskmelon frost Mildew, reticulate pattern disease；Grapevine anthracnose, powdery mildew, downy mildew；Leaf spot of peanut；Tea tree anthracnose, net cake disease；Dish soft rot.

The bactericidal composition of the present invention is considered being used especially for control cucumber downy mildew, rice blast, rice banded sclerotial blight Disease, bacterial blight of rice, bacterial leaf streak of rice, cucumber bacterial stripe, citrus bacterial canker disease, melon and fruit pinta, Solanaceae are made Object bacterial wilt, bacterial wilt of peanut, canker of tomato, tomato late blight, nail head spot of tomato, anthracnose of tomato etc..

The bactericidal composition of the present invention can be used as foliage fungicide in crop protection, also be used to dressing seed and being used as soil Fungicide.

The bactericidal composition of the present invention can be used for protecting plant, plant part, plant propagation material and the plant then grown Sundries official.

Use of the bactericidal composition of the present invention for protecting plant, plant propagation material and the plant organ then grown On the way.

The bactericidal composition of the present invention is used to handle seed the use invaded with the plant pathogenic fungi for protecting seed to carry On the way.

The bactericidal composition of the present invention, can handle all plants and plant part." plant " refers to all plants and plant Population, such as ideal and undesirable wild plant, cultivated plant and plant variety are (regardless of whether by plant variety or plant Cultivate the protection of human rights profit).Cultivated plant and plant variety can be the plants obtained by Sterile culture and breeding method, this A little methods can be aided with or be supplemented with one or more biological technique methods, such as use dihaploid, protoplast fusion, random With orthomutation, molecule or genetic marker, or use bioengineering and genetic engineering method.Plant part refers to all of plant Part and organ above and below the ground, such as bud, leaf, Hua Hegen, such as leaf, needle, stem, branch, flower, fructification, fruit and kind Son and root, bulb and rhizome.It crop and nourishes and generates and case of propagation material, such as transplants, bulb, rhizome, running roots Plant part is also belonged to seed.

Term " plant propagation material " is interpreted as referring to all plant parts for having fertility, such as seed, can use In breeding the latter and vegetable matter such as cuttage item or stem tuber（Such as potato）.Therefore, plant used herein Part includes plant propagation material.It is mentioned that such as seed, root, fruit, stem tuber, bulb, rhizome and plant part.It waits for The germination plant and effective plant inhibited after germinateing in soil or after emergence.Young plant can be before transplantation by being impregnated into Row whole or Local treatment are protected.

Currently preferred plant propagation material is seed.The bactericidal composition of the present invention is also particularly suitable for processing seed. Crop damage caused by most harmful fungoid is due to during storage or after sowing and in plant germination process Or caused by the infringement of the seed after germination.Since the root and branch of growth period plant are especially sensitive and even if small damage It can result in the death of plant.Another aspect of the present invention provides a kind of method of protection seed and germinating plants, and this method makes After planting or without additionally applying crop protection agents after plant germination or at least significantly additionally using crop protection agents.It is another Aspect, using the present invention bactericidal composition optimize used in reactive compound amount, with farthest provide seed and The protection of germinating plants is against the invasion of plant pathogenic fungi, and plant itself will not be by used reactive compound Damage.

Therefore, the present invention with the bactericidal composition of the present invention also more particularly to by handling seed to protect seed and hair The method that bud plant invades from plant pathogenic fungi.The invention further relates to bactericidal compositions according to the present invention in processing kind Son is to protect seed and germinating plants from the purposes of plant pathogenic fungi.

Harm budding after plant plant pathogenic fungi control mainly by using crop protection agents processing soil and The aerial part of plant carries out.Healthy issuable influence in view of crop protection agents on environment and humans and animals, It is therefore desirable to reduce the amount of application of reactive compound to the greatest extent.

Bactericidal composition according to the present invention is suitable for protection in agricultural, in greenhouse, in forestry or gardening-or vinegrowing The seed of any plant variety of kind application.Particularly, the seed form used is cereal（Such as wheat, barley, rye, black Wheat, millet, oat）, corn, cotton, soybean, rice, potato, sunflower, Kidney bean, coffee, beet, peanut, rape, olive Olive, cocoa, sugarcane, tobacco, vegetables（Such as tomato, cucumber, onion and lettuce）, turfgrass and decoration use plant.Cereal and vegetable The processing of the seed of vegetables is vital.

Active constituent gram bacterium in the bactericidal composition of the present invention is strong and zinc thiazole is independent or is applied with suitable dosage form For seed.It is preferably handled in the state of substantially stabilized so that processing will not cause any damage to seed.In general, Random time point that can be between picking and sowing carries out processing seed.Commonly used seed detaches from plant and from fringe Axis, shell, stem, epidermis, hair or pulp are isolated.Therefore, it is possible to use for example, low to water content by picking, cleaning and drying In 15% seed.Alternatively, it is also possible to for example with water process after using drying, the seed then dried once more.

The medicament of the method for seed treatment, such as can there are, diluent liquid or solid-like or without dilution directly general Seed is immersed in the method for making medicament be impregnated with seed in liquid condition solution, is blended in solid chemicals or liquid preparation and seed Together, being coated processing makes the method for the surface of the seed attachment medicament, the methods of sprinkling near seed while plantation.

Plant part and the plant organ then grown are any of the plant generated by plant propagation material such as seed Part.Plant part, plant organ and plant can also be benefited from by the way that bactericidal composition is applied to plant propagation material institute The pathogenic damage of acquisition is protected.The plant organ grown behind certain plants part and certain places can also regard plant propagation as Material, its own can be applied with bactericidal composition（Or processing）；To by processed plant part and processed plant Plant, other plant parts and the other plant organs that organ generates can also be benefited from by applying bactericidal composition With.

Bactericidal composition of the invention is applied to pathogenic or saprophytic in the required place prevention soil prevented or cultivation medium Fungi and bacterium purposes.

The bactericidal composition of the present invention can also be used to prevent or control a variety of pathogenic or saprophytic in soil or cultivation medium Fungi and bacterium.The example of the aquacultural fungal pathogen of soil-borne includes Alternaria（Alternaria spp.）, Ascochyta (Ascochyta spp.), Botrytis cinerea（Botrytis cinerea, Cercospora（Cercospora spp., ergot （Clavicepspurpurea）, standing grain cochliobolus（Cochliobolussativus）, Colletotrichum（colletotrichum Spp., Epicoccum（Epicoccum spp., F.graminearum schw（Fusariumgraminearum）, rice evil seedling chain spore （Fusariummoniliforme）, fusarium oxysporum（Fusariumoxysporum, fusarium moniliforme （Fusariumproliferatum）, fusariun solani (Fusariumsolani), dimension glue chain spore (Fusariumsubglitinans), Helminthosporium (Helminthosporiumspp) avenges rotten microtorr bacterium (Microdochiumnivale), Penicillium (Pencilliumspp), Phoma (Phoma spp.), wheat nuclear cavity bacteria (Pyrenophoragraminea), rice blast Pyricularia Sacc. (Pyriculariaoryzae), Rhizoctonia solani Kuhn (Rhizoctoniasolani), Rhizoctonia cerealis (Rhizoctoniacerealis), Sclerotinia (Sclerotinia Spp.), Septoria (Septoria spp.), silk axis smut (Sphacelothecareilliana), Tilletia (Tilletia spp.), meat spore core coral bacterium (Typhula incarnate), hidden smut (Urocystisocculta) are black Powder Pseudomonas (Ustilago spp.), Verticillium (Verticillium spp.), Phytophthora（Phytophthora), grass is rotten mould It is withered to belong to（Pythium), Peronospora（Peronospora）, Pseudoperonospora (Pseudoperonospora）.

Under normal circumstances, soil germ can generate a large amount of thalline, as long as condition develops favorably and host pathogen growth It is susceptible again, germ mass propagation and can infect host, and in the presence of susceptible host, these germs can enter The lasting pathogenic phase, with the continuous cropping of crop mass propagation spread, but later nutrient be depleted or edaphic condition such as temperature, When humidity etc. is unfavorable to germ, germ can enter rest period again.In the absence of susceptible host, soil-borne disease bacterium is in the soil It can survive, in addition to soil germ has extensive host range, moreover it is possible to be deposited on the root surface of non-host or the broken branches and fallen leaves It is living, it is undivided with saprophytic competitiveness with it.But different germs are discrepant, as sickle-like bacteria almost may be used in the soil It is survived with indefinite duration.

Cultivation medium of the present invention is the supporter for referring to make crops take root, grow, such as：Soil, water etc., Specific raw material can use for example sand, float stone, vermiculite, diatomite, agar-agar, gelling material, polymer substance, asbestos, Sawdust, bark etc..

The method that medicament is applied into soil, such as liquid preparation is diluted in water or is not diluted and is directly applied to plant The methods of in the root of body or the rice seedling bed of seedling, granule is disseminated to the side in the root of plant or the rice seedling bed of seedling Method has the method that pulvis, water dispersible granules etc. are sprayed in soil prior to seeding and are integrally mixed with soil, before sowing or plants It will be sprayed in implantation hole, seed furrow after pulvis, water-dispersible grain dilution agent before kind plant, carrying out method of sowing etc..

The bactericidal composition of the present invention can also be used to prevent fruit vegetables storing period disease, and obtain unexpected collaboration The effect of synergy.Such as the fruit rot caused by following pathogen：

Phytophthora（), such as phytophthora infestans (Phytophthorainfestans), phytophthora sojae kaufmann&gerdemann Phytophthora （Phytophthoramegasperma), foot rot of citrus bacterium（Phytophthoraparasitica)；

Peronosporaceae（Peronosporaceae) disease such as Plasmopara viticola（Plasmoparaviticola), Peronospora disease Bacterium（Peronospora）；

Pythium（Pythium）Such as scraping and printing unit（Pythiumaphanidermatum).

According to the present invention, after harvest and the disease of storage period can be for example by caused by following fungi：Colletotrichum kind, Such as banana thorn disk spore (Colletotrichum musae), Colletotrichum gloeosporiodes (Colletotrichum Gloeosporioides), capsicum thorn disk spore (Colletotrichum coccodes)；Fusarium kind, such as half-naked reaping hook Bacterium (Fusarium semitectum), fusarium moniliforme (Fusarium moniliforme), Fusarium solani (Fusarium Solani), Fusarium oxysporum (Fusarium oxysporum)；Verticillium dahliae belongs to kind, such as cocoa verticillium sp (Verticillium theobromae)；Nigrospora belongs to kind；Botrytis kind, such as the pathogen of Botrytis cinerea (Botrytis cinerea)；Geotrichum, such as geotrichum candidum (Geotrichum candidum)；Phomopsis kind, Natal Phomopsis (Phomopsis natalensis)；Diplodia kind, such as two spore of citrus color (Diplodia citri)；Alternaria kind, example Such as Altemaria citri (Alternaria citri), mutually every Alternariaspp (Alternaria alternata)；Phytophthora kind, example Such as phytophthora brown rot of citrus (Phytophthora citrophthora), strawberry phytophthora (Phytophthora fragariae), dislike Phytophthora (Phytophthora cactorum), Phytophthora nicotianae (Phytophthora parasitica);Septoria (Septoria spp.), such as Septoria depressa；Mucor (Mucor spp.), such as pyriform Mucor (Mucor piriformis)；Chain sclerotinia sclerotiorum belong (Monilinia spp.), such as fruit life chain sclerotinia sclerotiorum (Monilinia Fructigena), drupe chain sclerotinia sclerotiorum (Monilinia laxa)；Venturia (Venturia spp.), such as the black star of apple Bacterium (Venturia inaequalis), Pear scab (Venturia pyrina)；Rhizopus (Rhizopus spp.), such as Rhizopus stolonifer (Rhizopus stolonifer), Rhizopus oryzae (Rhizopus oryzae)；It is small from shell category (Glomerella Spp.), such as enclose small from shell (Glomerella cingulata)；Sclerotinia (Sclerotinia spp.), such as fruit life Sclerotinite (Sclerotinia fruiticola)；Long beak shell category (Ceratocystis spp.), such as unusual long beak shell (Ceratocystis paradoxa)；Penicillium (Penicillium spp.), such as penicillium funiculosum (Penicillium Funiculosum), penicillium expansum (Penicillium expansum), Penicillium digitatum (Penicillium digitatum), Italian mould (Penicillium italicum)；The long spore category of disk (Gloeosporium spp.), such as the long spore of white disk (Gloeosporium album), Gloeosporium perennans, the long spore (Gloeosporium of fruit life disk fructigenum),Gloeosporium singulata；Shell snake spore category (Phlyctaena spp.), such as Phlyctaena vagabunda；Cylindrocarpon (Cylindrocarpon spp.), such as apple column spore (Cylindrocarpon mali)；It crawls handle Mould category (Stemphyllium spp.), such as day lily stemphylium botryosum (Stemphyllium vesicarium)；Star check shell spore Belong to (Phacydiopycnis spp.), such as Phacydiopycnis malirum；Thiclaviopsis (Thielaviopsis Spp.), such as singular root string strain is mould (Thielaviopsis paradoxy)；Aspergillus (Aspergillus spp.) is such as black Aspergillus (Aspergillus niger), Aspergillus carbonerius (Aspergillus carbonarius);Nectria (Nectria ), such as the red shell bacterium of dry cancer clump (Nectria galligena) spp.；Stockless Peziza (Pezicula spp.).

Can also when plant or plant part are grown using bactericidal composition according to the present invention to protect harvest after Stock.

Processing method according to the present invention can also be used for the invasion for protecting stock from fungi and microorganism.According to this hair It is bright, it is understood as referring to term " stock " to have originated from natural life cycle and it is desirable that the plant of long-term preservation or animality is come The natural materials in source and its processed form.Stock, such as plant of plant origin or part thereof, as stem, leaf, stem tuber, Seed, fruit or seed, can be with the state of fresh harvesting or with form processing such as (pre-) dry, wetting, crushing, grinding, pressure System or baking are protected.Can also be timber, thick timber form such as construction timber, electric pole and fence；Or final product form, such as by Furniture or article made of timber.The stock of animal origin is animal skin, leather, hair, hair etc..Composition according to the present invention It can prevent the invasion such as burn into colour fading or mouldy of the fungi or bacterium of storage period.It is preferred that " stock " is understood as referring to plant The natural materials in object source and its form processing, more preferable fruit and its form processing, as the operatic circle, drupe, soft fruit and Citrus fruit and its form processing.

The present invention provides a kind of plant of control plant, plant part, plant propagation material and the plant organ then grown The method of object disease fungus, this method include by the bactericidal composition of the present invention so that agronomy is effective and basic plant-less toxicity is applied Dosage applied with seed treatment, foliage applying, stem, is impregnated with, instiled, poured into a mould, sprayed, sprayed, dusting, the methods of distribution or smoke It is administered to plant, plant part, plant propagation material or plant and is growing or needs the soil grown wherein or cultivation matchmaker In Jie.

A method of prevention plant pathogen includes with agronomy by the bactericidal composition of the present invention effectively and substantially without plant The amount of application of object toxicity applied with seed treatment, foliage applying, stem, is impregnated with, instiled, poured into a mould, sprayed, sprayed, dusting, distribution or The methods of smoke is administered to plant pathogen and/or its environment or plant, plant part, plant propagation material and subsequent length In the plant organ, soil or cultivation medium, material or the space that go out.

Another aspect of the invention also provides purposes of the bactericidal composition in improving plant health.

The present invention also provides the purposes that the bactericidal composition is used to increase crop leaf chlorophyll content.

The present invention also provides the purposes that the bactericidal composition is used to increase crop yield.

The term " improving plant health " of the present invention refers to improved crop characteristic, including：Emergence, crop yield, albumen Matter content, more flourishing root system, tiller increase, plant height increases, blade becomes larger, the basal leaf of death is less, stronger point Tiller, leaf green be greener, pigment content, photochemical vitality, need less fertilizer, need less seed, more fertile point Tiller uprightly increases compared with prematurity, cereal more early ripe, the lodging of less plant, branch growth increase, plant vigor increase, plant It is sprouted with early stage.

, it is surprising that compared with the activity that single compound may obtain, when the bactericidal composition of the present invention is used for When plant, seed or growth place, gram bacterium is strong and zinc thiazole increases the improvement of plant health in a manner of super adduction.

The present invention also provides a kind of methods improving plant health, and bactericidal composition of the present invention is acted on target The seed of useful plant, target useful plant.

The present invention also provides a kind of methods improving plant health, prior to seeding by the expected seed for growing target plant And/or it is handled with bactericidal composition of the present invention after vernalization.

The present invention bactericidal composition crop applicatory and/or crop seed include but not limited to：It is rice, wheat, big Wheat, rye, oat, corn, cotton, soybean, red bean, Kidney bean, peanut, beet, rape, sunflower, tobacco, eggplant, tomato, horse Bell potato, cucurbita pepo, cucumber；It is preferred that rice, wheat, corn, cotton, soybean, peanut, eggplant, tomato, cucurbita pepo, cucumber；It is more excellent Select rice, wheat, corn.

The bactericidal composition of the present invention can be applied with itself, be applied with its dosage form or use form prepared therefrom With.It is administered by conventional methods, such as poured, sprayed, atomizing, broadcasted sowing, dust, Waterless Seed Dressing, wet-mixing kind, slurry Liquid is dressed seed.

Gram bacterium is strong and zinc thiazole can be applied jointly or separately.In the case of separate administration, the application of each active constituent Can simultaneously or the part as processing sequence carry out one by one, wherein in the case where applying successively preferably with several minutes It is administered to the interval of a couple of days.

The bactericidal composition of the present invention can be applied by different processing methods, these methods are for example：

Liquid comprising the bactericidal composition is sprayed onto to the aerial part of the plant；

Dusting mixes particle or powder in the soil, is sprayed in the surrounding plants, and the tree injection or smearing the case where Under；

Cladding or film coated are carried out to the seed of plant；

When for postharvest fruit and vegetable anti-corrosive fresh-keeping.

Those skilled in the art can be according to actual conditions and needs, especially in accordance with the property of pending plant or crop Matter and the seriousness of germ adjust rate of application.

Generally for leaf portion processing：0.1-10000g/ha, preferably 10-1000 g/ha, more preferable 50-500 g/ha；It is right For dipping or application of instiling, the dosage can also even reduce, especially when using inert base such as asbestos or pearl When rock；

For seed treatment：2-5000g/100kg seeds, preferably 3-1000g/100kg seeds；

For soil or processing used for ponds：0.1-10000g/ha, preferably 1-1000g/ha.

It is fresh-keeping for postharvest fruit and vegetable, 200-2000 times of liquid can be diluted, is leached after soaking fruit.

Gram bacterium of the present invention is strong to combine/combined administration with zinc thiazole.Including separating, sequentially or simultaneously application gram bacterium is strong and thiophene Azoles zinc.Preferably, described gram of bacterium is strong is combined as with zinc thiazole comprising the strong form with the composition of zinc thiazole of gram bacterium.

The present invention composition can be based on dosage form, i.e., each substance has mixed in composition, composition at Point can also unit dose form provide, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with this hair Based on the dosage form of bright offer.

As a further improvement on the present invention, bactericidal composition of the invention can be configured to the arbitrary agent agriculturally allowed Type.

As a further improvement on the present invention, the dosage form of bactericidal composition of the invention is suspending agent, seed coat agent, wettable Pulvis, microcapsule suspending agent, coated granule, squeezes out granule, missible oil, microemulsion, aqueous emulsion, effervescent tablet, surpasses water dispersible granules Low capacity liquor, suspension emulsion.

In bactericidal composition of the present invention, including gram bacterium is strengthened and zinc thiazole, filler and/or surfactant.

Bactericidal composition of the present invention, wherein the strong content with zinc thiazole of gram bacterium accounts for the 5%-90% of bactericidal composition.

The bactericidal composition, wherein the strong content with zinc thiazole of gram bacterium accounts for the 10%-80% of bactericidal composition.

The bactericidal composition, wherein the strong content with zinc thiazole of gram bacterium accounts for the 10%-60% of bactericidal composition.

According to the present invention, term " filler ", which refers to, can be combined with reactive compound or combines to make it easier for being administered to Object（Such as plant, crop or careless class）Natural or synthetic organic or inorganic compound.Therefore, the filler is preferably It is inert, it is acceptable at least to should be agricultural.The filler can be solid or liquid.

It can having for example as solid media materials'use in the present invention：Phyteral powdery type（Such as soy meal, shallow lake Of powder, grain dust, wood powder, bark powder, sawdust, walnut shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem Grain extracts the residue etc. after plant essence）, paper, sawdust, crush the synthesized polymer body, clay class of synthetic resin etc.（Such as it is high Ridge soil, bentonite, acid china clay etc.）, talcum powder class.Silica（Such as diatomite, silica sand, mica, aqueous silicic acid, calcium silicates）, Activated carbon, natural mineral matter class（Float stone, attapulgite and zeolite etc.）, fire diatomite, sand, plastics medium etc.（Such as poly- second Alkene, polypropylene, polyvinylidene chloride etc.）, potassium chloride, calcium carbonate, the inorganic mineral powder of calcium phosphate etc., ammonium sulfate, phosphoric acid Chemical fertilizer, the clay fertilizer of ammonium, urea, ammonium chloride etc., these substances can be used alone or two or more is mixed.

It can be selected in llowing group of materials as liquid media materials'use in the present invention, such as water, aromatic series Compound, such as dimethylbenzene, toluene or alkylnaphthalene；Chlorinated aromatic compounds of group or chlorinated aliphatic, for example, chlorobenzene, vinyl chloride or Dichloromethane；Aliphatic hydrocarbon, such as hexamethylene or paraffin, such as petroleum distillate；Alcohol, for example, butanol or ethylene glycol and their ether and Ester；Ketone, such as acetone, methyl ethyl ketone, methyl iso-butyl ketone (MIBK) or cyclohexanone；Intensive polar solvent, such as dimethylformamide or diformazan Base sulfoxide；Or water.The meaning of liquefied gas filler or carrier is interpreted as under normal temperature and atmospheric pressure being gaseous liquid, Such as aerosol spray, such as halogenated hydrocarbons or butane, propane, nitrogen and carbon dioxide.

To make the emulsification of active ingredient compound, dispersion and/or wetting, it can use surfactant such as can be with Enumerate fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol or phenol Phosphate, the aliphatic ester of polyalcohol, alkane aromatic sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonates, the branch shape of macromolecule comb shape are total Polymers, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, grease, fatty alcohol and ethylene oxide condensation The polyacrylates such as object, alkyltaurate, protein hydrolysate.Suitable oligosaccharide object or polymer, such as based on independent Vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or its with for example（It is polynary）Alcohol or（It is polynary）The combination of amine.

To make active ingredient compound dispersion stability, attachment and/or combination, such as xanthans, silicic acid can be used Magnalium, gelatin, starch, methyl cellulose, polyvinyl alcohol, polyvinyl acetate and natural phospholipid（Such as cephalin and lecithin）With And the adjuvants such as synthetic phospholipid, bentonite, sodium lignin sulfonate.

The wherein optional spent glycol of antifreezing agent, propylene glycol, glycerine, sorbierite.Deflocculant as suspension product The adjuvant such as naphthalene sulfonic acid polymer, polymeric phosphate can be used.

Organic silicon defoamer can be used as antifoaming agent.

The colorant that can be used, such as inorganic pigment, such as iron oxide, titanium oxide and Prussian blue；And organic pigment/ Dyestuff：Alizarin dyes, azo dyes and metallized phthalocyanine dye；And trace element, such as molysite, manganese salt, boron salt, mantoquita, cobalt Salt, molybdenum salt and zinc salt.

Optionally, also may include other annexing ingredients, for example, protecting colloid, adhesive, thickener, thixotropic agent, bleeding agent, Stabilizer, screening agent.

The preparation of the present invention can be prepared by known way mixes the reactive compound with conventional additives. The conventional additives such as conventional extender and solvent or diluent, emulsifier, dispersant, and/or adhesive or fixative, Wetting agent, waterproofing agent, if it is desired, can also include drier and colorant, stabilizer, pigment, antifoaming agent, preservative, increasing Thick dose, water and other processing aids.

These compositions include not only can be by suitable equipment such as spraying or dusting equipment immediately suitable for pending Object, but also be included in be applied to before object and need the concentration commercial composition being diluted.

The present invention's is strong and zinc thiazole can also be applied with other active ingredient combinations containing gram bacterium, such as expanding activity It composes or prevents to form resistance.Other active constituents such as fungicide, bactericide, attractant, insecticide, mite killing Agent, nematicide, growth regulator, herbicide, safener, fertilizer or semiochemical etc..

Reactive compound gram bacterium is strong and zinc thiazole can be administered simultaneously, or applies respectively or sequential application, when separate administration Sequence is on the result of prevention usually without influence.

The bactericidal composition of the present invention can be based on dosage form, i.e., each substance has mixed in composition, composition Ingredient can also unit dose form provide, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with Based on dosage form provided by the invention.

For the bactericidal composition of the present invention in the case where reducing reactive compound and applying total amount, having to harmful fungoid improves activity （It is synergistic）.And the bactericidal composition of the present invention shows that the bacterium of tolerance also has excellent kill to existing fungicide Bacterium effect.

In the present invention, gram bacterium is strengthened and zinc thiazole use in conjunction can either increases relative to each single dose and combination of two Add chlorophyll content of plant, improve crop photosynthesis efficiency, and crop yield can be increased, to improve economic and society's effect Benefit.The bactericidal composition of the present invention is compared with the activity that single compound may obtain, when the bactericidal composition of the present invention is used for When plant, seed or growth place, captan and zinc thiazole increase the improvement of plant health in a manner of super adduction.

Bactericidal composition of the present invention provide to harmful fungoid and bacterium have good effect and solving reduce dosage rate and/ Or the agricultural methods and composition of the problem of enhancing activity profile and/or drug resistance management.

Specific implementation mode

Below with reference to embodiment, the invention will be further described：

Example of formulations

1 30% gram of bacterium of embodiment strengthens+30% zinc thiazole wettable powder

Gram bacterium strong 30%

Zinc thiazole 30%

Lauryl sodium sulfate 10%

Sodium lignin sulfonate 5%

White carbon 10%

Kaolin complements to 100%

By active constituent, various auxiliary agents and filler etc. by formula proportional components mix, after ultra-fine pulverizer disintegrating to get to 30% gram of bacterium strengthens+30% zinc thiazole wettable powder.

2 10% grams of bacterium of embodiment strengthen+20% zinc thiazole wettable powder

Gram bacterium strong 10%

Zinc thiazole 20%

Calcium dodecyl benzene sulfonate 5%

Sodium lignin sulfonate 5%

Sucrose complements to 100%

By active constituent, various auxiliary agents and filler etc. by formula proportional components mix, after ultra-fine pulverizer disintegrating to get to 10% gram of bacterium strengthens+20% zinc thiazole wettable powder.

3 2% grams of bacterium of embodiment strengthen+1% zinc thiazole missible oil

Gram bacterium strong 2%

Zinc thiazole 1%

Ethoxylated castor oil 5%

Calcium dodecyl benzene sulfonate 3%

SOLVESSO^TM200 complement to 100%

Mentioned component is proportionally prepared, uniform phase is uniformly mixing to obtain.

4 20% grams of bacterium of embodiment strengthen+30% zinc thiazole wettable powder

Gram bacterium strong 20%

Zinc thiazole 30%

Lauryl sodium sulfate 10%

Sodium lignin sulfonate 5%

White carbon 10%

Kaolin complements to 100%

Said components are mixed in proportion, and grinds, crush, are prepared into wettable powder.

5 10% grams of bacterium of embodiment strengthen the dispersible oil-suspending agent of+20% zinc thiazole

Gram bacterium strong 10%

Zinc thiazole 20%

Nekal 5%

MODIFIED LIGNOSULPHONATE 5%

Xanthans 1%

Bentonite 1%

Glycerine 5%

PVP-K30 1%

Water 3%

Soybean oil complements to 100%

Dispersible oil-suspending agent is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion.

6 0.5% grams of bacterium of embodiment strengthen+0.5% zinc thiazole electrostatic oil agent

Gram bacterium strong 0.5%

Zinc thiazole 0.5%

Calcium dodecyl benzene sulfonate 5%

N-Methyl pyrrolidone complements to 100%

Said components are mixed evenly to obtain homogeneous phase.

7 15% grams of bacterium of embodiment strengthen+30% thiazole zincium suspension agent

Gram bacterium strong 15%

Zinc thiazole 30%

Fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium 5%

MODIFIED LIGNOSULPHONATE 5%

Xanthans 1%

Bentonite 1%

Glycerine 5%

PVP-K30 1%

Water complements to 100%

Suspending agent is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion.

8 2% grams of bacterium of embodiment strengthen+10% thiazole gel of zinc

Gram bacterium strong 2%

Zinc thiazole 10%

Lauryl sodium sulfate 5%

Gelling agent 1%

Water complements to 100%

Said components are ground in the ball mill of stirring, obtain delicate suspensions.The stabilization for being diluted with water to obtain active component is outstanding Thus supernatant liquid obtains 2% gram of bacterium and strengthens+10% thiazole gel of zinc.

9 30% grams of bacterium of embodiment strengthen+10% zinc thiazole suspension seed-coating agent

Gram bacterium strong 30%

Zinc thiazole 10%

Fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium 5%

MODIFIED LIGNOSULPHONATE 5%

Bentonite 3%

Soybean oil complements to 100%

Suspension seed-coating agent is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion.

10 20% grams of bacterium of embodiment strengthen+20% zinc thiazole coated granule

Gram bacterium strong 20%

Zinc thiazole 20%

Polyethylene glycol 3%

The silicic acid 1% of high degree of dispersion

Calcium carbonate complements to 100%

It in a mixer, will be on levigate active constituent even spread to the carrier soaked by polyethylene glycol.It can obtain by this method Obtain dust-free coated granules agent.

11 5% grams of bacterium of embodiment strengthen+30% thiazole zincium suspension agent

Gram bacterium strong 5%

Zinc thiazole 30%

Fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium 5%

MODIFIED LIGNOSULPHONATE 5%

Xanthans 1%

Bentonite 1%

Glycerine 5%

PVP-K30 1%

Water complements to 100%

Above-mentioned each component is mixed, stirring is to obtaining transparent homogeneous phase.

12 30% grams of bacterium of embodiment strengthen+6% zinc thiazole and squeeze out granule

Gram bacterium strong 30%

Zinc thiazole 6%

Sodium lignin sulfonate 4%

Carboxymethyl cellulose 2%

Kaolin complements to 100%

Active component is mixed and ground with auxiliary agent, mixture is soaked with water.The mixture is squeezed out, is then done in the air stream It is dry.

13 10% grams of bacterium of embodiment strengthen+40% zinc thiazole suspension seed-coating agent

Gram bacterium strong 10%

Zinc thiazole 40%

Fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium 5%

MODIFIED LIGNOSULPHONATE 5%

Xanthans 1%

Bentonite 1%

Glycerine 5%

PVP-K30 1%

Water complements to 100%

14 1% grams of bacterium of embodiment strengthen+20% zinc thiazole suspension emulsion

Gram bacterium strong 1%

Zinc thiazole 20%

SOLVESSO^TM200 10%

Ethoxylated castor oil 4%

Fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium 5%

MODIFIED LIGNOSULPHONATE 5%

Xanthans 1%

Bentonite 1%

Glycerine 5%

Water complements to 100%

Gram bacterium is strengthened and is dissolved in SOLVESSO^TMIn 200, ethoxylated castor oil is added, obtains the strong oil phase of gram bacterium；

Fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium, MODIFIED LIGNOSULPHONATE, zinc thiazole and water are mixed in proportion It closes, obtains uniformly, obtaining the suspending agent of zinc thiazole after ground and/or high speed shear.

The oil phase strengthened containing gram bacterium is added to the aqueous suspension agent containing zinc thiazole, obtains suspension emulsion.

15 5% grams of bacterium of embodiment strengthen+50% zinc thiazole water dispersible granules

Gram bacterium strong 5%

Zinc thiazole 50%

MODIFIED LIGNOSULPHONATE 5%

Lauryl sodium sulfate 5%

Urea 5%

Kaolin complements to 100%

By gram bacterium, strong, zinc thiazole, dispersant, wetting agent, disintegrant and filler are mixed according to the formula uniformly, by air-flow powder It is broken into wettable powder；It adds a certain amount of water mixing extruding and makes material.5% gram of bacterium, which is obtained, after dry sieve strengthens+50% thiazole Zinc water dispersible granules.

16 2% grams of bacterium of embodiment strengthen+30% zinc thiazole wettable powder

Gram bacterium strong 2%

Zinc thiazole 30%

Lauryl sodium sulfate 1%

Sodium lignin sulfonate 1%

Kaolin complements to 100%

17 50% grams of bacterium of embodiment strengthen+50% zinc thiazole

Gram bacterium strong 50%

Zinc thiazole 50%

By gram bacterium, strong, zinc thiazole is proportionally uniformly mixed.

Proportioning in above example is weight per distribution ratio.

Biological test example

After the Fungicidal compounds mixing of different cultivars, three kinds of type of action are typically exhibited：Summation action, synergistic effect and antagonism Effect.But it is specially which kind of effect, it is unpredictable, only just it can know that by many experiments.Compound synergic is formulated well, by In significantly improving practical control effect, the usage amount of active constituent is reduced, to greatly delay the generation of resistance.

One, virulence is tested：

The toxicity index of each medicament and the co-toxicity coefficient of mixture are calculated according to Sun Yunpei's Method（CTC values）, when CTC≤80, then combine Object shows antagonism, when 80<CTC<120, then composition show summation action, when CTC >=120, then composition shows Go out synergistic effect.

Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100

B in the percentage composition+B medicament toxicity index * mixtures of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixtures Percentage composition

Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI) * 100

Phytopathogen and host plant used in greenhouse virulence test

Host plant	Disease title
		Cucumber	Bacterial wilt
Cucumber	Bacterial angular leaf spot
		Rice	Bacterial leaf-blight
Citrus	Canker
		Rice	Bacterial stripe
Rice	Rice blast
		Cucumber	Downy mildew

The toxicity test of 1 Cucumber wilt of test example

Subjects：Cucumber wilt bacterium

It is cucumber sense bacterial wilt kind for studying object.20 are sowed after vernalization per basin, 10 plant of 3 consistent leaf phase of selection wherein growing way Cucumber is spare.On the basis of pilot study, according to agent activity, 12 series mass concentration are set.Liquid is uniformly sprayed In blade face to whole wettings, it is advisable with not dripping, waits for that liquid natural air drying is spare.Often handle 1 basin, 4 repetitions.Chemicals treatment After for 24 hours, even spraying is inoculated with ralstonia solanacearum spore suspension on cucumber leaves.

The configuration method of spore suspension：Experiment is seeded in pathogen on oatmeal tomato juice agar, in It is cultivated 5-7 days under the conditions of 25 DEG C -27 DEG C, black light lamp induced meristem spore generates, and lower conidium is washed from culture medium, 2- is used in combination 4 layers of filtered through gauze centrifuge (3000 revs/min), abandon supernatant, and the conidium under precipitating is outstanding with 20 aqueous solutions of 0.025%Tween Floating, blood counting chamber counts, and it is spare that spore suspension concentration is adjusted to 2 × 105 spore/ml.

It is dark that (relative humidity 95% or more keep blade face have moisture condensation, temperature 25 DEG C -26 DEG C) is moved in humidistat after inoculation Under the conditions of moisturizing culture for 24 hours.Then in 25 DEG C -26 DEG C, 12h alternation of light and darkness (intensity of illumination 20000lx), relative humidity 85%- 7d is cultivated under conditions of 90%.When blank control disease leaf rate reaches 50% or more, the incidence of whole strain blade is investigated in classification. Stage division is：

0 grade：Whole strain is disease-free；

1 grade：There is brown scab；

3 grades：There is typical spindle scab, lesion area accounts for 5% or less whole leaf area；；

5 grades：Typical scab, lesion area account for the 6%-25% of whole leaf area；

7 grades：Typical scab, lesion area account for the 26%-50% of whole leaf area；

9 grades：Typical scab, lesion area account for 50% or more of whole leaf area.

Drug effect computational methods：

Control effect is converted into niqueMin (Y), and liquor strength (mg/L) is converted into logarithm (x), and poison is calculated with least square method Power equation and inhibit in concentration EC50, according to co-toxicity coefficient method (《Pesticide experimental technique and evaluation method》, Huang Guoyang chief editors, 2000, Beijing, Chinese agriculture publishing house：217-218).

Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) × 100,

In theoretical toxicity index (TTI)=A medicament toxicity index × mixture in percentage composition+B medicament toxicity index × mixture of A The percentage composition of B,

Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI)] × 100.

When CTC≤80, then composition shows as Hangzhoupro effect short of money, and as 80 < CTC < 120, then composition shows as being added and make With as CTC >=120, then composition shows as synergistic effect.

The virulence test result of 1 present invention prevention Cucumber wilt of table

1 test result of table shows to prevent Cucumber wilt, and gram bacterium is strong and zinc thiazole in weight proportion is 20:1-1:20 range In when, co-toxicity coefficient both illustrates that the mixture within the scope of this shows as gain effect all 120 or more.

The toxicity test of 2 bacterial blight of rice of test example

Subjects：Bacterial blight of rice

It is rice susceptible variety for studying object.Clip same area growing way is placed in unanimously with petiolate blade in culture dish, is protected It is wet spare.On the basis of pilot study, according to agent activity, 12 series mass concentration are set.Blade is prepared in advance Liquid in fully infiltrate 5s, drain extra liquid, natural air drying, by moisturizing culture after processing label.Uniformly it is sprayed on blade face To whole wettings, it is advisable with not dripping, waits for that liquid natural air drying is spare.Often handle 30 blades, 4 repetitions.Chemicals treatment is for 24 hours Afterwards, the bacteria cake for taking 5mm is beaten with inoculator, have mycelia is inoculated in processing central vane on one side.25 DEG C -26 DEG C after inoculation, relatively 3d is cultivated under conditions of humidity 85%-90%.Scab is measured, and calculates preventive effect.Disease index computational methods are as follows：

Remaining computational methods is the same as embodiment 1.

The virulence test result of 2 present invention prevention bacterial blight of rice of table

2 test result of table shows to prevent bacterial blight of rice, and gram bacterium is strong and zinc thiazole in weight proportion is 20:1-1:20 model When enclosing inner, co-toxicity coefficient illustrates that mixture of the two within the scope of this shows as gain effect all 120 or more.

The toxicity test of 3 cucumber bacterial angular leaf spot of test example

Subjects：Cucumber bacterial angular leaf spot

It is cucumber sense bacterial angular leaf spot kind for studying object.Sow 20 after vernalization per basin, selection wherein growing way it is consistent 10 3 leaf phase cucumber of strain are spare.On the basis of pilot study, according to agent activity, 12 series mass concentration are set.Liquid is equal The even blade face that is sprayed on is advisable to whole wettings with not dripping, and waits for that liquid natural air drying is spare.Often handle 1 basin, 4 repetitions.Medicament After processing for 24 hours, even spraying is inoculated with cucumber bacterial angular leaf spot spore suspension on cucumber leaves.

It is dark that (relative humidity 95% or more keep blade face have moisture condensation, temperature 25 DEG C -26 DEG C) is moved in humidistat after inoculation Under the conditions of moisturizing culture for 24 hours.Then in 25 DEG C -26 DEG C, 12h alternation of light and darkness (intensity of illumination 20000lx), relative humidity 85%- 7d is cultivated under conditions of 90%.When blank control disease leaf rate reaches 50% or more, the incidence of whole strain blade is investigated in classification.

Drug effect computational methods：

Remaining computational methods is the same as test example 1.

The virulence test result of 3 present invention prevention cucumber bacterial angular leaf spot of table

3 test result of table shows to prevent cucumber bacterial angular leaf spot, and gram bacterium is strong and zinc thiazole in weight proportion is 20:1-1:20 Range in when, co-toxicity coefficient both illustrates that the mixture within the scope of this shows as gain effect all 120 or more.

The toxicity test of 4 cucumber downy mildew of test example

Subjects：Cucumber downy mildew

It is cucumber downy mildew disease kind for studying object.20 are sowed after vernalization per basin, 10 plant of 3 consistent leaf phase of selection wherein growing way Cucumber is spare.On the basis of pilot study, according to agent activity, 12 series mass concentration are set.Liquid is uniformly sprayed In blade face to whole wettings, it is advisable with not dripping, waits for that liquid natural air drying is spare.Often handle 1 basin, 4 repetitions.Chemicals treatment After for 24 hours, even spraying is inoculated with cucumber downy mildew spore suspension on cucumber leaves.

It is dark that (relative humidity 95% or more keep blade face have moisture condensation, temperature 25 DEG C -26 DEG C) is moved in humidistat after inoculation Under the conditions of moisturizing culture for 24 hours.Then in 25 DEG C -26 DEG C, 12h alternation of light and darkness (intensity of illumination 20000lx), relative humidity 85%- 7d is cultivated under conditions of 90%.When blank control disease leaf rate reaches 50% or more, the incidence of whole strain blade is investigated in classification. Remaining computational methods is the same as test example 1.

The virulence test result of 4 present invention prevention cucumber downy mildew of table

4 test result of table shows to prevent cucumber downy mildew, and gram bacterium is strong and zinc thiazole in weight proportion is 20:1-1:20 range In when, co-toxicity coefficient both illustrates that the mixture within the scope of this shows as gain effect all 120 or more.

The toxicity test of 5 bacterial leaf streak of rice of test example

Subjects：Bacterial leaf streak of rice

Remaining computational methods is the same as embodiment 1.

The virulence test result of 5 present invention prevention bacterial leaf streak of rice of table

5 test result of table shows to prevent bacterial leaf streak of rice, and gram bacterium is strong and zinc thiazole in weight proportion is 20:1-1:20 Range in when, co-toxicity coefficient both illustrates that the mixture within the scope of this shows as gain effect all 120 or more.

The toxicity test of 6 rice blast of test example

Subjects：Rice blast

Remaining computational methods is the same as embodiment 1.

The virulence test result of 6 present invention prevention rice blast of table

6 test result of table shows to prevent rice blast, and gram bacterium is strong and zinc thiazole in weight proportion is 20:1-1:20 range In when, co-toxicity coefficient both illustrates that the mixture within the scope of this shows as gain effect all 120 or more.

Experiment 7：The toxicity test of citrus bacterial canker disease

Mandarin tree seedling is selected, is sprayed under 50PSI pressure with potter spray towers, per basin about 5mL, each medicament setting 12 concentration gradients.Bacterium is connect after chemicals treatment for 24 hours, the sick leaf infected by citrus bacterial canker disease will be picked up from mandarin tree seedling uniformly Shake off conidium to be inoculated with, then citrus seedling is put into greenhouse and cultivates.According to the morbidity of citrus bacterial canker disease point after 7d Grade standard complete stool investigates disease index, and calculates control effect, concentration EC50 in then being inhibited with least square method calculating, then according to Sun Yunpei's Method calculates co-toxicity coefficient (CTC).

The virulence test result of 7 present invention prevention citrus bacterial canker disease of table

7 test result of table shows to prevent mandarin tree canker, and gram bacterium is strong and zinc thiazole in weight proportion is 20:1-1:20 model When enclosing inner, co-toxicity coefficient illustrates that mixture of the two within the scope of this shows as gain effect all 120 or more.

8 chlorophyll content of rice of test example measures

Test material：Rice

Test method：Choose health, full rice paddy seed, through 0.1% mercuric chloride surface sterilizing, 10 min, after use distilled water flushing 4-5 times, filter paper suck dry moisture is used in combination.Then, the rice paddy seed of processing is grouped, every group 100, is used respectively described in following table Soak seed 36h at 28 DEG C of medicament, wherein setting clear water as blank control；Distilled water flushing is used after the completion of seed soaking, after taking-up 3 times.Later Laboratory sowing is carried out respectively and is cultivated.

When seedling grows to four leaves wholeheartedly, each processing blade is taken respectively, measures its chlorophyll content.Experiment is repeated 4 times, and is taken Average value.Specific chlorophyll measurement method according to《Plant physiology experiment instructs (second edition)》（Chen Jianxun chief editors, south China reason Work university press publishes）Described in method be measured, measure the OD value under two wavelength of 663nm and 645nm respectively, It is returned to zero with 80% acetone.Chlorophyll content is calculated according to gained OD value., result is as shown in table 8 below.

8 rice seedling measuring chlorophyll content result of table

Medicament	Drug concentration (g/L)	Chlorophyll (mg/g)	Chlorophyll increment rate (%)	It is expected that increment rate（%）
					Clear water	---	1.533	-	-
Gram bacterium is strong	10	1.612	5.15	-
					Gram bacterium is strong	5	1.61	5.02	-
Gram bacterium is strong	4	1.597	4.17	-
					Gram bacterium is strong	2.5	1.592	3.84	-
Gram bacterium is strong	2.0	1.587	3.52	-
					Gram bacterium is strong	1.5	1.583	3.26	-
Gram bacterium is strong	1.0	1.581	3.13	-
					Gram bacterium is strong	0.5	1.578	2.93	-
Zinc thiazole	0.5	1.665	8.61	-
					Zinc thiazole	1.0	1.678	9.45	-
Zinc thiazole	1.5	1.682	9.71	-
					Zinc thiazole	2.0	1.684	9.84	-
Zinc thiazole	2.5	1.689	10.1	-
					Zinc thiazole	4	1.76	14.8	-
Zinc thiazole	5	1.762	14.9	-
					Zinc thiazole	10	1.765	15.1	-
Gram bacterium is strong+zinc thiazole	10+0.5	24.653	1.89	13.31
					Gram bacterium is strong+zinc thiazole	10+1	26.743	1.92	14.11
Gram bacterium is strong+zinc thiazole	10+1.5	27.233	1.93	14.35
					Gram bacterium is strong+zinc thiazole	10+2.0	28.333	1.94	14.48
Gram bacterium is strong+zinc thiazole	10+2.5	29.283	1.96	14.72
					Gram bacterium is strong+zinc thiazole	10+4	35.733	2.06	19.18
Gram bacterium is strong+zinc thiazole	10+5	36.183	2.06	19.28
					Gram bacterium is strong+zinc thiazole	10+10	36.963	2.08	19.47
Gram bacterium is strong+zinc thiazole	0.5+10	30.293	1.97	17.58
					Gram bacterium is strong+zinc thiazole	1+10	31.183	1.99	17.75
Gram bacterium is strong+zinc thiazole	1.5+10	31.693	2.00	17.86
					Gram bacterium is strong+zinc thiazole	2.0+10	34.293	2.04	18.08
Gram bacterium is strong+zinc thiazole	2.5+10	34.633	2.04	18.36
					Gram bacterium is strong+zinc thiazole	4+10	34.693	2.04	18.64
Gram bacterium is strong+zinc thiazole	5+10	35.653	2.06	19.36

As shown in Table 8, the strong weight ratio with zinc thiazole of gram bacterium is 20:1-1:In the range of 20, rice seedling chlorophyll is contained Amount, which is improved, obtains synergistic effect compared to single dose.

Claims

1. a kind of bactericidal composition, it is characterised in that：Active constituent is formed by gram bacterium is strong with zinc thiazole, wherein gram bacterium is strengthened and thiazole The weight percent of zinc is 20:1-1:20, preferably 15:1-1:15,10:1-1:10, further preferably 5:1-1:5.

2. bactericidal composition according to claim 1, it is characterised in that：Described gram bacterium is strong and zinc thiazole quality sum accounts for institute State the 5%-90% of Bactericidal composite amount of substance, more preferable 10%-80%, more preferable 20%-60%.

3. bactericidal composition according to claim 1, it is characterised in that：The bactericidal composition, dosage form be suspending agent, Seed coat agent, wettable powder, water dispersible granules, microcapsule suspending agent, coated granule, squeezes out granule, is missible oil, micro- suspension emulsion Emulsion, aqueous emulsion, effervescent tablet, ultra low volume liquids.

4. bactericidal composition according to claim 1, it is characterised in that：Also include filler and/or surfactant.

5. a kind of method of prevention plant pathogen, it is characterised in that：Bactericidal composition described in claim 1 is acted on into plant Object pathogenic bacteria and/or its environment or plant, plant part, plant propagation material and the plant organ then grown, soil or It cultivates in medium, material or space.

6. a kind of method of prevention plant pathogen, it is characterised in that：Described in claim 1 gram of bacterium is strengthened with zinc thiazole simultaneously Using or apply respectively or sequential application.

7. a kind of method of prevention plant pathogen, it is characterised in that：It is wanted before infecting or by right after infecting in plant The bactericidal composition described in 1 is asked to act on plant pathogen and/or its environment or plant, plant part, plant propagation material In the plant organ, soil or cultivation medium, material or the space that then grow.

8. a kind of method of prevention plant pathogen, it is characterised in that：Including by bactericidal composition described in claim 1 with agriculture Learn the amount of application of effective and basic plant-less toxicity applied, be impregnated with seed treatment, foliage applying, stem, instiled, poured into a mould, sprayed, The methods of spraying, dusting, distribution or smoke are applied to plant pathogen and/or its environment or plant, plant part, plant In propagating materials and the plant organ then grown, soil or cultivation medium, material or space.

9. bactericidal composition described in claim 1 is true on Cereal, veterinary antibiotics, ornamental plant and grapevine for preventing The purposes of bacterium and bacterium.

10. bactericidal composition described in claim 1 is yellow on Cereal, veterinary antibiotics, ornamental plant and grapevine for controlling Melon downy mildew, rice blast, rice sheath blight disease, bacterial blight of rice, bacterial leaf streak of rice, cucumber bacterial stripe Disease, citrus bacterial canker disease, melon and fruit pinta, solanaceous crops bacterial wilt, bacterial wilt of peanut, canker of tomato, tomato late blight, tomato are brown Pinta, the purposes of anthracnose of tomato.

11. bactericidal composition described in claim 1 is for protecting plant, plant propagation material and the plant organ then grown Purposes.

12. bactericidal composition described in claim 1 is applied to the required place prevention soil prevented or causes a disease in cultivation medium Or the purposes of saprophytic fungi and bacterium.

13. bactericidal composition described in claim 1 is used to handle seed the phytopathogen invasion to protect seed carrying Purposes.

14. bactericidal composition described in claim 1 is used to protect the purposes of the fruits and vegetables after harvest.

15. bactericidal composition described in claim 1 is for protecting stock in storage period from fungi or the use of bacterial invasion On the way.

16. purposes of the bactericidal composition described in claim 1 in improving plant health.

17. bactericidal composition described in claim 1 is used to increase the purposes of crop leaf chlorophyll content.

18. bactericidal composition described in claim 1 is used to increase the purposes of crop yield.