CN109717192A - A kind of composition pesticide - Google Patents

Abstract

The present invention relates to a kind of composition pesticide, the composition pesticide contains active constituent fluorine thiazole pyrrole ethyl ketone and chitosan, and the weight ratio of fluorine thiazole pyrrole ethyl ketone and chitosan is 50:1-1:50.The invention further relates to the composition pesticides to be used to prevent and treat Cereal, veterinary antibiotics, pathogenic epiphyte and/or improves the purposes of plant health on ornamental plant and grapevine.

Description

A kind of composition pesticide

Technical field

The present invention relates to a kind of composition pesticide, further relate to the composition pesticide prevention and treatment plant pathogenic fungi and/or Improve the purposes of plant health.

Background technique

About pesticide activity, especially to the protection of crop, the key problem that conducts a research in the field first is that how Improve the performance of pesticide activity component, the especially performance in terms of bioactivity and keeps this active property within a certain period of time Energy.

It will lead to plant pathogenic fungi appearance in many cases with the single individual agricultural chemical compound of application repeatedly There is drug resistance that is natural or adapting to the agricultural chemical compound in fast selective, that is, plant pathogenic fungi.

Fluorine thiazole pyrrole ethyl ketone (Oxathiapiprolin)；CAS:1003318-67-9；Chemical name are as follows: 1- (4- [4- [(5RS) -5- (2,6- difluorophenyl) -4,5- dihydro -1,2- oxazole -3- base] -1,3- thiazol-2-yl] -1- piperidyl) -2- [5- methyl -3- (trifluoromethyl) -1H- pyrazol-1-yl] ethyl ketone.Its molecular structural formula are as follows:

Fluorine thiazole pyrrole ethyl ketone is the first piperidyl thiazole isoxazoles fungicide of Du Pont's exploitation, is disclosed in In CN101888843C；It has special efficacy to downy mildew and late blight.The action site of fluorine thiazole pyrrole ethyl ketone is single, fungicide resistance Action Committee thinks, with middle high-level resistance risk, needs to carry out resistance management.

Summary of the invention

It is an object of the present invention to provide in the case where the reduction of the application total amount of reactive compound to harmful fungoid or Bacterium, which has, improves active composition (synergistic composition), to reduce the rate of application of known activity compound and improve it Activity profile.

It is a further object to provide the compositions for improving plant health.Healthier plant can obtain higher Plant fruit yield and/or higher plant or fruit quality；And it further decreases in agricultural and reactive compound is applied Dosage further helps in and avoids developing drug resistance to each pesticide.

Composition pesticide provided by the invention solves the problems, such as above-mentioned part or all of.On the one hand, the present invention provides one The composition pesticide of kind prevention and treatment plant pathogenic fungi；On the other hand, the present invention provides a kind of combinations for improving plant health Object.

The present invention provides a kind of composition pesticide, the composition is by carrying out two for fluorine thiazole pyrrole ethyl ketone and chitosan Member compounding, the composition made is in prevention and treatment plant pathogenic fungi and/or improves on plant health with gain effect.Herein " gain effect " refer to that composition of the invention is substantially higher than the active adduction of each single reactive compound；In the presence of can not Prediction, necessary being synergistic effect, rather than just active adduction.

Composition pesticide provided by the invention adopts the following technical solutions to achieve:

A kind of composition pesticide, containing active constituent fluorine thiazole pyrrole ethyl ketone and chitosan, the fluorine thiazole pyrrole ethyl ketone It is 50:1-1:50, preferably 40:1-1:40, further preferred 30:1-1:30 with the weight ratio of chitosan, still more preferably 25:1-1:25, more preferable 20:1-1:20, more preferable 15:1-1:15, more preferable 10:1-1:10, more preferable 5:1-1:5.

It was found that simultaneously, i.e., jointly or separately applying fluorine thiazole pyrrole ethyl ketone and chitosan, or successively apply fluorine thiazole Pyrrole ethyl ketone and chitosan, which enable, preferably prevents and treats plant pathogenic fungi and/or improvement than each compound is administered alone Plant health.

When reactive compound is present in composition pesticide of the invention with specific weight ratio, synergistic effect is especially bright It is aobvious.But the weight ratio of the reactive compound in composition pesticide of the present invention can change in a certain range.

The weight ratio of fluorine thiazole pyrrole ethyl ketone and chitosan in the present invention such as can also be 50:1,49:1,48:1, 47:1、46:1、45:1、44:1、43:1、42:1、41:1、40:1、39:1、38:1、37:1、36:1、35:1、34:1、33:1、 32:1、31:1、30:1、29:1、28:1、27:1、26:1、25:1、24:1、23:1、22:1、21:1、20:1、19:1、18:1、 17:1、16:1、15:1、14:1、13:1、12:1、11:1、10:1、9:1、8:1、7:1、6:1、5:1、4:1、3:1、2:1、1:1、 1:2、1:3、1:4、1:5、1:6、1:7、1:8、1:9、1:10、1:11、1:12、1:13、1:14、1:15、1:16、1:17、1:18、 1:19、1:20、1:21、1:22、1:23、1:24、1:25、1:26、1:27、1:28、1:29、1:30、1:31、1:32、1:33、1: 34、1:35、1:36、1:37、1:38、1:39、1:40、1:41、1:42、1:43、1:44、1:45、1:46、1:47、1:48、1:49 Or 1:50.

The quality sum of fluorine thiazole pyrrole ethyl ketone and chitosan accounts for the pesticide combination substance in the composition pesticide The 5%-90% of amount, preferably 10%-80%, more preferable 20%-60%.

In composition pesticide of the invention, the quality sum of the fluorine thiazole pyrrole ethyl ketone and chitosan accounts for the pesticide group Close object by weight can also be, for example, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 65%, 70%, 75%, 80%, 85% or 90%.

A method of prevention and treatment plant pathogenic fungi and/or improvement plant health act on the composition pesticide In plant pathogen and/or its environment or plant, plant part, plant propagation material or the then plant organ, the soil that grow In earth or cultivation medium, material or space.

A method of prevention and treatment plant pathogenic fungi and/or improvement plant health act on the composition pesticide In plant or plant part.

A method of prevention and treatment plant pathogenic fungi and/or improvement plant health act on the composition pesticide The plant organ in plant propagation material or then grown.

A method of prevention and treatment plant pathogenic fungi and/or improvement plant health act on the composition pesticide In soil or cultivation medium.

A method of prevention and treatment plant pathogenic fungi and/or improvement plant health gather fluorine thiazole pyrrole ethyl ketone and chitin Sugar be administered simultaneously or respectively application or sequential application.

A method of prevention and treatment plant pathogenic fungi and/or improve plant health, before infecting or can be invaded in plant The composition pesticide is acted on into plant pathogen and/or its environment after dye or plant, plant part, plant are numerous Grow material or the plant organ, soil or the cultivation medium, material or space that then grow in.

A method of prevention and treatment plant pathogenic fungi and/or improving plant health, including by pesticide combination of the invention Object is with agronomy is effective and the amount of application of basic plant-less toxicity is applied with seed treatment, foliage applying, stem, be impregnated with, instil, pour The methods of note, injection, spraying, dusting, distribution or smoke are applied to plant pathogen and/or its environment or plant, plant portion Divide, in plant propagation material or the plant organ then grown, soil or cultivation medium, material or space.

A method of prevention and treatment plant pathogenic fungi and/or improvement plant health exist the expected seed for growing plant Synergistic a effective amount of composition pesticide processing of the invention is used before sowing and/or after vernalization.

A method of protection seed, including the fluorine thiophene after making seed prior to seeding and/or vernalization with cooperative effective quantity Azoles pyrrole ethyl ketone and chitosan contact.

The composition pesticide is for preventing and treating Cereal, veterinary antibiotics, pathogenic epiphyte on ornamental plant and grapevine Purposes.

The composition pesticide is used to improve the purposes of plant health.

The composition pesticide is used to protect the purposes of plant, plant propagation material or the plant organ then grown.

The place that the composition pesticide is prevented and treated needed for being applied to is pathogenic or saprophytic in soil or cultivation medium to prevent and treat Pathogenic epiphyte purposes.

The plant pathogenic fungi invasion that the composition pesticide is used to handle seed to protect seed to prevent seed from carrying And/or improve the purposes of plant health.

The composition pesticide is used to protect the purposes of the fruits and vegetables after harvest.

The purposes that the composition pesticide is used to that stock to be protected to infect in storage period from plant pathogenic fungi.

A kind of composition pesticide, it includes fluorine thiazole pyrrole ethyl ketones and chitosan and filler and/or surfactant.

A kind of composition pesticide can be configured to any dosage form agriculturally allowed.The composition pesticide, dosage form It can be suspending agent, seed coat agent, suspoemulsion, wettable powder, water dispersible granules, microcapsule suspending agent, coated granule, extrusion Granula, missible oil, microemulsion, aqueous emulsion, effervescent tablet or ultra low volume liquids.

The fluorine thiazole pyrrole ethyl ketone can exist with different crystal forms.

The composition pesticide can be applied without dilution, and application can also be diluted with water.

Composition pesticide of the invention has very strong activity to various plant pathogenic fungis, and can be to by pathogenic Property fungus-caused plant disease prevention and treatment play very strong preventive effect.

The composition pesticide is for preventing and treating Cereal, veterinary antibiotics, pathogenic epiphyte on ornamental plant and grapevine Purposes.

Composition pesticide of the invention plants the wide scopes such as such as Basidiomycetes, Ascomycetes, Oomycete and deuteromycetes Object pathogenic epiphyte has excellent activity.

Oomycete, including Phytophthora (Phytophthora) disease, such as phytophthora infestans (Phytophthora Infestans), phytophthora sojae kaufmann&gerdemann (Phytophthora megasperma), phytophthora parasitica (Phytophthora Parasitica), camphor tree phytophthora (Phytophthora cinnamomi) and Phytophthora capsici (Phytophthora capsici)；It is rotten Mould category disease such as scraping and printing unit (Phythiumaphanidermatum)；Peronosporaceae disease such as P. viticola (Plasmoparaviticola), peronospora (Peronospora) (including Peronosporatabacina (Peronosporatabacina) and Peronospora parasitic bacterium (Peronospora parasitica)), Pseudoperonospora (Pseudoperonospora spp.) (including bacterium of downy mildew of cucumber (Pseudoperonospora cubensis) and disk stalk it is mould Bacterium germ (Bremialactucae))；

Ascomycetes, including Alternaria (Alternaria) disease such as tomato early blight bacterium (Alternaria ) and alternaria brassica (Alternaria brassicae) solani；Ball seat Pseudomonas (Guignardia) disease such as grape ball Seat bacterium (Guignardia bidwelli), Venturia (Venturia) disease such as apple black star bacteria (Venturiainaequalis)；Septoria (Septoria) disease such as glume blight bacterium (Septoria nodorum) and leaf Blight bacterium (Septoria tritici)；Powdery mildew disease such as Erysiphe (Erysiphe spp.) (including wheat powdery mildew Bacterium (Erysiphegraminis) and trailing plants Powdery Mildew (Erysiphepolygoni)), grape powdery mildew (Uncinula Necatur), cucumber powdery mildew's pathogen (Sphaerotheca fuligena) and apple mildew bacterium (Podosphaera leucotricha)；Wheat Phyllostachys pubescens (Pseudocercosporella herpotrichoides)；Botrytis disease (Botrttis) such as Botrytis cinerea germ (Botrytis cinerea), Monilinia fructicola (Moniliniafructicola)； Sclerotinia (Sclerotinia) species disease such as Sclerotinia sclerotiorum (Sclerotiniasclerotiorum), rice blast Bacterium (Magnaporthegrisea), grape branch-rot bacterium (Phomopsisviticola), Helminthosporium (Helminthosporium) species disease such as Exserohilum turcicum (Helminthosporiumtriticirepentis), Reticulate pattern germ (Pyrenophorateres)；Anthrax disease is for example small from shell category germ (Glomerella) or colletotrichum germ (Colletotrichum category) (such as fine strain of millet anthrax bacteria (Colletotrichumgraminicola) and watermelon anthrax bacteria And gaeumannomyces graminis (Gaeumannomycesgraminis) (Colletotrichumorbiculare))；

Basidiomycetes, including the rest fungus disease as caused by Rust (Puccinia category) (such as Puccinia recondita (Pucciniarecondita), strip rust bacteria (Pucciniastriiformis), leaf rust (Pucciniahordei), puccinia graminis bacterium (Pucciniagraminis) and handle rest fungus (Pucciniaarachidis)), coffee rest fungus (Hemileiavastatrix) and Phakopsora pachyrhizi sydow (Phakopsorapachyrhizi)；Other pathogens include Rhizoctonia (Rhizoctonia category) species (such as Rhizoctonia solani Kuhn (Rhizoctoniasolani) and redness of the skin or complexion hyphal cluster germ (Rhizoctoniaoryzae))；

Deuteromycetes, such as Botrytis (Botrytis), Pyricularia Sacc. (Pyricularia), Helminthosporium (Helminthosporium), Fusarium (Fusarium), Septoria (Septoria), Cercospora (Cercospora), chain Lattice spore category (Alternaria), Pyricularia Sacc. (Pyricularia), false Cercosporalla (Pseudocercospora).

Composition pesticide of the invention is also to bacterium such as tobacco smoke pollution (Pseudomonas tabaci), pears fire epidemic disease Germ (Erwiniaamylovora), xanthomonas campestris (Xanthomonascampestris), pseudomonas syringae (Pseudomonassyringae) and other strains have resistance activity.

The suitable crop plants of composition pesticide of the invention specifically include that cereal crops, for example, wheat, barley, oat, Naked barley, triticale, rice, corn, sorghum and millet；Vine crop, such as Table Grape and vinifera；Field crop, example Such as rape (Canola), sunflower；Sugar beet, sugarcane, soybean, peanut (peanut), tobacco, clover, clover, Hu Zhi Son, clover and vetch；Pip fruit, such as apple, pears, crabapple, loquat, hawthorn and quince；Stone fruit, example Such as peach, cherry, plum, apricot, honey peach；Citrus fruit, such as lemon, bitter orange, orange, shaddock, mandarin orange (orange) and kumquat； Radicant and field crop, such as arithoke, common beet and sugar beet, carrot, cassava, ginger, ginseng, horseradish, Europe Indian Epimeredi Herb, potato, little radish, turnip, sweet potato, turnip and Chinese yam；Bulb bearing plant, such as garlic, leek, onion and It is verdant；Leaf vegetables plant, for example, mustard seed Chinese Ixeris (rocket salad), celery, celery, Chinese celery, witloof (thatch dish), fennel, bile ingredients and Leaf lettuce, parsley, red witloof, rheum officinale, spinach and sugared lettuce；Btassica (cabbage) leaf vegetables, such as broccoli, cauliflower (broccoli), Brussels sprouts, cabbage, Chinese cabbage, cauliflower, wild cabbage, collard, root-mustard, leaf mustard and green vegetables；Bean (succulence or without juice) such as lupin (including semen viciae fabae, kidney bean, Kidney bean, Hua Dou, scarlet runner bean, snap beans, wide leaf vegetables Beans and wax bean), Kidney bean (including red bean, asparagus bean, cow gram, black cowpea, long bean, cowpea, mung bean, cowpea, urd bean and Speciality cowpea), semen viciae fabae, chick-pea, melon ear, sword bean, Lens culinaris and pea (including kidney bean, edible podded pea, field pea, pea, Green pea, snow bean, sweet tea beans, pigeonpea and soybean)；Fruit vegetables, such as eggplant, ground-cheery, Solanum muicatum and capsicum (including bell pepper, Capsicum, capsicum for cooking, pimiento, pimento；Small tomato and tomato)；Cucurbit class vegetables, such as chocho (fruit), wax gourd, Chinese holly Rafter watermelon, cucumber, young cucumber, marrow squash (including cucurbit, lagenaria sicerariae), sponge gourd, gumbo, balsam apple, dioecism momordica root, balsam pear and China Cucumber, muskmelon, cucurbita pepo and winter squash and watermelon；Berries, such as blackberry, blueberry, the red fruit certain kind of berries, the purplish blue certain kind of berries, blueberry, Cranberry, the wild certain kind of berries, Loganberry, raspberry and strawberry；Set raw nut, such as almond, beech nut, Brazil nut, butternut, cashew nut, Chinese chestnut, fibert (filbert), hickory nut, Queensland nut, pecan and English walnut；Tropical fruit (tree) and other crops, such as banana, plantain, awns Fruit, coconut, pawpaw, avocado, lichee, American aloe, coffee, cocoa, sugarcane, oil palm, sesame, rubber and fragrance；Fibre crops, example Such as cotton, flax and hemp；Sod grass (including season type and shitivi type sod grass).

Composition pesticide of the invention is especially suitable for prevention and treatment tomato, Chinese cabbage, cucumber, capsicum, potato, grape, balsam pear, lettuce Lettuce, tobacco, turfgrass, rice, peanut, mandarin tree, pear tree, wheat, barley, oat, pathogenicity is true on seeding corn and other crops plant The purposes of bacterium.

Composition pesticide of the invention is considered being used especially for control Botrytis (Botrytis), Pyricularia Sacc. (Pyricularia), Helminthosporium (Helminthosporium), Fusarium (Fusarium), Septoria (Septoria), Cercospora (Cercospora), Alternaria (Alternaria), false Cercosporalla (Pseudocercospora), Rhizoctonia (Rhizoctonia), camel spore rest fungus category (Hemileia), Puccinia (Puccinia), Phakopsora (Phakopsora), black Powder Pseudomonas (Llstilaginalcs), Venturia (Venturia), Erysiphe (Erysiphe), Podosphaera (Podosphaera), chain sclerotinia sclerotiorum belong (Monilinia), Uncinula (Uncinula), mycosphaerella (Mycosphaerella), Phytophthora (Phytophthora), pythium (Pythium), Plasmopara (Plasmopara), frost The plants such as mould category (Peronospora), Pseudoperonospora (Pseudoperonospora), disk stalk mould (Bremialactucae) Pathogenic epiphyte.

Composition pesticide of the invention is especially suitable for preventing and treating phytophthora infestans (Phytophthora Infestans), phytophthora sojae kaufmann&gerdemann (Phytophthoramegasperma), foot rot of citrus bacterium (Phytophthoraparasitica), camphor tree phytophthora (Phytophthoracinnamomi), phytophthora capsici (Phytophthoracapsici), Pythium aphanidermatum (Pythiumaphanidermatum), Plasmopara viticola (Plasmoparaviticola), Peronospora tabacina germ (Peronosporatabacina), Peronospora parasitic germ (Peronospora parasitica), bacterium of downy mildew of cucumber (Pseudoperonosporacubensis), disk obstruct mould germ (Bremialactucae) and tobacco smoke pollution (Pseudomonas tabaci).

Composition pesticide of the invention is particularly suitable for the prevention and treatment late blight of potato, potato late blight, grape downy mildew Disease, citrus bacterial canker disease, pear scab, cucumber downy mildew, wildfire, mandarin tree shot hole, apple tree brown spot, capsicum Epidemic disease and tomato late blight.

On the other hand, the present invention also provides the purposes that the composition pesticide is used to improve plant health.

Term " improving plant health ", which refers to, improves crop characteristic, comprising: emergence, crop yield increase, protein content Increase, more flourishing root system, tiller increase, plant height increases, blade becomes larger, dead basal leaf is more preferable, Ye Yanse is greener, Pigment content increases, photochemical vitality enhancing, need less fertilizer, need less seed, more fertile tiller, compared with Prematurity, cereal more early ripe, the lodging of better plant, branch growth increases, vegetable active increases, plant uprightly increases and early stage It sprouts.

Term " crop yield increase " refers to that compared under the same terms, the yield increase by one of plant product is measurable Amount, applies composition pesticide of the invention compared with applying single compound, produces the effect of obvious gain.

There may be super plus (" collaboration ") effects for processing according to the present invention.For example, the pesticide group used according to the present invention It closes the rate of application of object and/or widens its field of activity and/or increase its activity, it is possible to obtain the following effect for improving plant health Fruit: better plant growth increases the tolerance of high temperature or low temperature, increases to the tolerance of arid or water or soil salt content Add, blooming performance improves, it is easier to it harvests, mature faster, higher yield rate, bigger fruit, higher plant height, The color of leaf is greener, blooms earlier, and the quality or nutritive value of the product of harvest are higher, and sugared concentration is higher in fruit, harvest Product storage stability and/or processability more preferably, these benefits have been more than the effect actually estimated.

The present invention provides a kind of methods of prevention and treatment plant pathogenic fungi and/or improvement plant health, by the agriculture Drug composition acts on plant or plant part.

Composition pesticide of the invention can handle all plants and plant part." plant " refers to all plants and plant Species group, such as ideal and undesirable wild plant, cultivated plant and plant variety are (regardless of whether by plant variety or plant The protection of object cultivation human rights benefit).Cultivated plant and plant variety can be the plant obtained by Sterile culture and breeding method, These methods can be aided with or be supplemented with one or more biological technique methods, for example, using dihaploid, protoplast fusion, with Machine and orthomutation, molecule or genetic marker, or use bioengineering and genetic engineering method.Plant part refers to the institute of plant Have part and an organ above and below the ground, such as bud, leaf, Hua Hegen, such as leaf, needle, stem, branch, flower, fructification, fruit and Seed and root, bulb and rhizome.It crop and nourishes and generates and case of propagation material, such as transplants, bulb, rhizome, fine crawl Branch and seed also belong to plant part.

Harm budding after plant plant pathogenic fungi control mainly by using crop protection agents processing soil and The aerial part of plant carries out.Healthy issuable influence in view of crop protection agents on environment and humans and animals, It is therefore desirable to reduce the amount of application of reactive compound to the greatest extent.

It on the other hand, will the present invention provides a kind of method of prevention and treatment plant pathogenic fungi and/or improvement plant health The plant organ that the composition pesticide acts on plant propagation material or then grows.

Term " plant propagation material " is interpreted as referring to all plant parts for having fertility, such as seed, can use In breeding the latter and vegetable matter such as cuttage item or stem tuber (such as potato).Therefore, plant used herein Part includes plant propagation material.It is mentioned that such as seed, root, fruit, stem tuber, bulb, rhizome and plant part.To The germination plant and effective plant inhibited after germinateing in soil or after emergence.Young plant can be before transplantation by being impregnated into Row whole or Local treatment are protected.Currently preferred plant propagation material is seed.

The composition pesticide be used for handle seed with protect seed to be carried plant pathogenic fungi invasion and/or Improve the purposes of plant health.

Most of damage of the crop plants as caused by plant pathogenic fungi sows seed during the storage of seed It is shortly had occurred into after soil, and during plant germination or after rudiment.The root and bud of plant in growth are special Insensitive, and even small damage can also lead to the death of whole plant.Therefore, at by using suitable seed Compositions-treated seed is managed to protect the plant in seed and rudiment that there is especially great meaning.

Active constituent fluorine thiazole pyrrole ethyl ketone and chitosan in composition pesticide of the invention is individually or with suitable system Dosage form formula is applied to seed.It is preferably handled in the state of substantially stabilized so that processing will not cause any damage to seed Evil.In general, can any point-in-time between picking and sowing carry out processing seed.Commonly used seed is separated from plant And it is isolated from cob, shell, stem, epidermis, hair or pulp.Therefore, it is possible to use for example by picking, cleaning and dry to containing Water is lower than 15% seed.Alternatively, it is also possible to for example with water process after using drying, the kind then dried once more Son.

The medicament of the method for seed treatment, such as can there are, diluent liquid or solid-like does not have to dilution directly general Seed is immersed in liquid condition solution the method for making medicament be impregnated with seed, is blended in solid chemicals or liquid preparation and seed Together, being coated processing makes the method for the surface of the seed attachment medicament, the methods of sprinkling near seed while plantation.

Plant part and the plant organ then grown are any of the plant generated by plant propagation material such as seed Part.Plant part, plant organ and plant also can benefit from by the way that composition pesticide is applied to plant propagation material institute The pathogenic damage of acquisition is protected.The plant organ grown behind certain plants part and certain places can also regard plant propagation as Material, its own can apply (or processing) with composition pesticide；To by processed plant part and processed plant Plant, other plant parts and the other plant organs that organ generates also can benefit from by applying composition pesticide With.

The present invention provides a kind of method for preventing and treating plant pathogenic fungi and/or improving plant health, and expection is grown plant The seed of object is handled with synergistic a effective amount of composition pesticide of the invention prior to seeding and/or after vernalization.

The present invention also provides a kind of methods of prevention and treatment plant pathogenic fungi and/or improvement plant health, including make seed It is contacted prior to seeding and/or after vernalization with the fluorine thiazole pyrrole ethyl ketone and chitosan of cooperative effective quantity.

Another aspect of the present invention provides a kind of method for protecting seed and germinating plants, this method make after planting or Crop protection agents are additionally applied without additional application crop protection agents or at least significant ground after plant germination.On the other hand, it utilizes Composition pesticide of the invention optimize used in reactive compound amount, farthest to provide seed and germinating plants The invasion against plant pathogenic fungi are protected, and plant itself not will receive the damage of used reactive compound.

Therefore, the present invention is also in particular to by handling seed with composition pesticide of the invention to protect seed and hair The method that bud plant invades from plant pathogenic fungi.The invention further relates to composition pesticides according to the present invention in processing kind Purposes of the son to protect seed and germinating plants to infect from plant pathogenic fungi.

Composition pesticide of the invention is suitable for protection to be applied in agricultural, in greenhouse, in forestry or in gardening or vinegrowing The seed of any plant variety.Particularly, the seed form used for cereal (such as wheat, barley, rye, triticale, Millet, oat), corn, cotton, soybean, rice, potato, sunflower, Kidney bean, coffee, beet, peanut, rape, olive, can Can, sugarcane, tobacco, vegetables (such as tomato, cucumber, onion and lettuce), turfgrass and decoration use plant.Cereal and greengrocery The processing of seed is vital.

Composition pesticide of the invention can also be used to prevent or control a variety of pathogenic or saprophytic in soil or cultivation medium Pathogenic epiphyte.

On the other hand, the place prevented and treated needed for composition pesticide of the present invention is applied to is to prevent and treat soil or cultivation matchmaker The purposes of pathogenic or saprophytic pathogenic epiphyte and/or improvement plant health in Jie.

The example of the aquacultural fungal pathogen of soil-borne includes Alternaria (Alternaria spp.), Ascochyta (Ascochyta spp.), Botrytis cinerea (Botrytis cinerea), Cercospora (Cercospora spp.), ergot (Clavicepspurpurea), standing grain cochliobolus (Cochliobolussativus), Colletotrichum (colletotrichum Spp.), Epicoccum (Epicoccum spp.), F.graminearum schw (Fusariumgraminearum), rice dislike seedling chain spore (Fusariummoniliforme), fusarium oxysporum (Fusariumoxysporum), fusarium moniliforme (Fusariumproliferatum), fusariun solani (Fusariumsolani), dimension glue chain spore (Fusariumsubglitinans), the rotten microtorr bacterium of Helminthosporium (Helminthosporiumspp), snow (Microdochiumnivale), Penicillium (Pencilliumspp), Phoma (Phoma spp.), wheat nuclear cavity bacteria (Pyrenophoragraminea), rice blast Pyricularia Sacc. (Pyriculariaoryzae), Rhizoctonia solani Kuhn (Rhizoctoniasolani), Rhizoctonia cerealis (Rhizoctoniacerealis), Sclerotinia (Sclerotinia Spp.), Septoria (Septoria spp.), silk axis smut (Sphacelothecareilliana), Tilletia It is (Tilletia spp.), meat spore core coral bacterium (Typhula incarnate), hidden smut (Urocystisocculta), black Powder Pseudomonas (Ustilago spp.), Verticillium (Verticillium spp.), Phytophthora (Phytophthora), grass are rotten mould It is withered to belong to (Pythium), Peronospora (Peronospora), Pseudoperonospora (Pseudoperonospora).

Under normal circumstances, soil germ can generate a large amount of thallus, as long as condition develops favorably and host pathogen growth Be again it is susceptible, germ mass propagation and can infect host, and in the presence of susceptible host, these germs can enter The lasting pathogenic phase, with the continuous cropping of crop mass propagation spread, but later nutrient be depleted or edaphic condition such as temperature, When humidity etc. is unfavorable to germ, germ can enter dormant period again.In the absence of susceptible host, soil-borne disease bacterium is in the soil It can survive, in addition to soil germ has extensive host range, moreover it is possible to be deposited on the root surface of non-host or the broken branches and fallen leaves It is living, it is undivided with it with saprophytic competitiveness.But different germs be it is discrepant, as sickle-like bacteria almost may be used in the soil It is survived with indefinite duration.

Cultivation medium of the present invention is the supporter for referring to make crops take root, grow, such as: soil, water etc., Specific raw material can be used for example sand, float stone, vermiculite, diatomite, agar-agar, gelling material, polymer substance, asbestos, Sawdust, bark etc..

The method that medicament is applied into soil, such as liquid preparation is diluted in water or is not diluted and is directly applied to plant The methods of in the root of body or the rice seedling bed of seedling, granule is disseminated to the side in the root of plant or the rice seedling bed of seedling Method has the method that pulvis, water dispersible granules etc. are sprayed in soil prior to seeding and are integrally mixed with soil, before sowing or plants Implantation hole will be sprayed on before kind of plant after pulvis, water-dispersible grain dilution agent, in seed furrow, carrying out method of sowing etc..

Composition pesticide of the invention can also be used to prevent and treat fruit vegetables storing period disease, and obtain unexpected collaboration The effect of synergy.

Therefore, the present invention provides the purposes that the composition pesticide is used to protect the fruits and vegetables after harvest.The present invention provides The purposes that the composition pesticide is used to that stock to be protected to infect in storage period from plant pathogenic fungi.

Stock infecting vulnerable to for example following pathogenic epiphyte in storage period:

Phytophthora (Phytophthora), such as phytophthora infestans (Phytophthora infestans), soybean Phytophthora germ (Phytophthoramegasperma), foot rot of citrus bacterium (Phytophthoraparasitica)；

Peronosporaceae (Peronosporaceae) disease such as Plasmopara viticola (Plasmoparaviticola), downy mildew Belong to germ (Peronospora)；

Pythium (Pythium) such as scraping and printing unit (Pythiumaphanidermatum).

It according to the present invention, can be for example by caused by following fungi with the disease of storage period after harvest: Colletotrichum kind, Such as banana pierces disk spore (Colletotrichum musae), Colletotrichum gloeosporiodes (Colletotrichum Gloeosporioides), capsicum thorn disk spore (Colletotrichum coccodes)；Fusarium kind, such as half-naked reaping hook Bacterium (Fusarium semitectum), fusarium moniliforme (Fusarium moniliforme), Fusarium solani (Fusarium Solani), Fusarium oxysporum (Fusarium oxysporum)；Verticillium dahliae belongs to kind, such as cocoa verticillium sp (Verticillium theobromae)；Nigrospora belongs to kind；Botrytis kind, such as the pathogen of Botrytis cinerea (Botrytis cinerea)；Geotrichum, such as geotrichum candidum (Geotrichum candidum)；Phomopsis kind, Natal Phomopsis (Phomopsis natalensis)；Diplodia kind, such as two spore of citrus color (Diplodia citri)；Alternaria kind, example Such as Altemaria citri (Alternaria citri), mutually every Alternariaspp (Alternaria alternata)；Phytophthora kind, example Such as phytophthora brown rot of citrus (Phytophthora citrophthora), strawberry phytophthora (Phytophthora fragariae), dislike Phytophthora (Phytophthora cactorum), Phytophthora nicotianae (Phytophthora parasitica)；Septoria (Septoria spp.), such as Septoria depressa；Mucor (Mucor spp.), such as pyriform Mucor (Mucor piriformis)；Chain sclerotinia sclerotiorum belong (Monilinia spp.), such as the raw chain sclerotinia sclerotiorum (Monilinia of fruit Fructigena), drupe chain sclerotinia sclerotiorum (Monilinia laxa)；Venturia (Venturia spp.), such as the black star of apple Bacterium (Venturia inaequalis), Pear scab (Venturia pyrina)；Rhizopus (Rhizopus spp.), such as Rhizopus stolonifer (Rhizopus stolonifer), Rhizopus oryzae (Rhizopus oryzae)；It is small from shell category (Glomerella Spp.), such as enclose small from shell (Glomerella cingulata)；Sclerotinia (Sclerotinia spp.), such as fruit are raw Sclerotinite (Sclerotinia fruiticola)；Long beak shell category (Ceratocystis spp.), such as unusual long beak shell (Ceratocystis paradoxa)；Penicillium (Penicillium spp.), such as penicillium funiculosum (Penicillium Funiculosum), penicillium expansum (Penicillium expansum), Penicillium digitatum (Penicillium digitatum), Italian mould (Penicillium italicum)；The long spore category of disk (Gloeosporium spp.), such as the long spore of white disk (Gloeosporium album), Gloeosporium perennans, the raw long spore (Gloeosporium of disk of fruit fructigenum),Gloeosporium singulata；Shell snake spore category (Phlyctaena spp.), such as Phlyctaena vagabunda；Cylindrocarpon (Cylindrocarpon spp.), such as apple column spore (Cylindrocarpon mali)；It crawls handle Mould category (Stemphyllium spp.), such as day lily stemphylium botryosum (Stemphyllium vesicarium)；Star check shell spore Belong to (Phacydiopycnis spp.), such as Phacydiopycnis malirum；Thiclaviopsis (Thielaviopsis Spp.), such as singular root string strain is mould (Thielaviopsis paradoxy)；Aspergillus (Aspergillus spp.) is such as black Aspergillus (Aspergillus niger), Aspergillus carbonerius (Aspergillus carbonarius)；Nectria (Nectria ), such as the red shell bacterium of dry cancer clump (Nectria galligena) spp.；Stockless Peziza (Pezicula spp.).

After composition pesticide according to the present invention can also being applied when plant or plant part grow to protect harvest Stock.

Processing method according to the present invention can also be used for the invasion for protecting stock from fungi and microorganism.According to this hair It is bright, it is understood as referring to term " stock " to have originated from natural life cycle and it is desirable that the plant of long-term preservation or animality is come The natural materials in source and its processed form.Stock, such as plant of plant origin or part thereof, as stem, leaf, stem tuber, Seed, fruit or seed, can be with the state of fresh harvesting or with form processing such as (pre-) dry, wetting, crushing, grinding, pressure System or baking are protected.It is also possible to timber, thick timber form such as construction timber, electric pole and fence；Or final product form, such as by Furniture or article made of timber.The stock of animal origin is animal skin, leather, hair, hair etc..Composition according to the present invention It can prevent the invasion such as burn into colour fading or mouldy of the fungi or bacterium of storage period.It is preferred that " stock " is understood as referring to plant The natural materials in object source and its form processing, more preferable fruit and its form processing, as the operatic circle, drupe, soft fruit and Citrus fruit and its form processing.

The present invention provides a kind of method for preventing and treating plant pathogenic fungi and/or improving plant health, by the pesticide Composition acts on plant pathogen and/or its environment or plant, plant part, plant propagation material and then grows In plant organ, soil or cultivation medium, material or space.

A method of prevention and treatment plant pathogenic fungi and/or improvement plant health act on the composition pesticide In plant or plant part.

A method of prevention and treatment plant pathogenic fungi and/or improvement plant health act on the composition pesticide The plant organ in plant propagation material and then grown.

A method of prevention and treatment plant pathogenic fungi and/or improvement plant health exist the expected seed for growing plant Synergistic a effective amount of composition pesticide processing of the invention is used before sowing and/or after vernalization.

A method of protection seed, including the fluorine thiophene after making seed prior to seeding and/or vernalization with cooperative effective quantity Azoles pyrrole ethyl ketone and chitosan contact.

A method of prevention and treatment plant pathogenic fungi and/or improvement plant health act on the composition pesticide In soil or cultivation medium.

A method of prevention and treatment plant pathogenic fungi and/or improvement plant health gather fluorine thiazole pyrrole ethyl ketone and chitin Sugar be administered simultaneously or respectively application or sequential application.

A method of prevention and treatment plant pathogenic fungi and/or improve plant health, before infecting or can be invaded in plant The composition pesticide is acted on into plant pathogen and/or its environment after dye or plant, plant part, plant are numerous Grow material and the plant organ, soil or the cultivation medium, material or space that then grow in.

A method of prevention and treatment plant pathogenic fungi and/or improving plant health, including by pesticide combination of the invention Object is with agronomy is effective and the amount of application of basic plant-less toxicity is applied with seed treatment, foliage applying, stem, be impregnated with, instil, pour The methods of note, injection, spraying, dusting, distribution or smoke are applied to plant pathogen and/or its environment or plant, plant portion Divide, in plant propagation material and the plant organ then grown, soil or cultivation medium, material or space.

Composition pesticide of the invention can be applied by different processing methods, these methods for example:

Liquid comprising the composition pesticide is sprayed onto the aerial part of the plant；

Dusting mixes particle or powder in the soil, sprays in the surrounding plants, and in tree injection or smearing In the case of；

Cladding or film coated are carried out to the seed of plant.

When for postharvest fruit and vegetable anti-corrosive fresh-keeping, 200-2000 times of liquid is usually diluted with water, is leached after soaking fruit.

Composition pesticide of the invention can be made into common dosage form, such as missible oil, wettable powder, suspending agent, liquid The dosage forms such as agent, granule, seed coat agent use, amount of application, according to the mixing ratio, meteorological condition, medicament of effective component Form, Dressing date, method of administration, application place, prevent and kill off object noxious livings, objective crop etc. it is different and variant.

Processing method of the invention can also be used for the aerial part of processing plant dry, stem or stalk for example in relation to plant, leaf, Colored and fruit.

Generally for leaf portion processing: 0.1-10000g/ha, preferably 10-1000g/ha, more preferable 50-500g/ha；For For dipping or application of instiling, the dosage can also even be reduced, especially when application inert base such as asbestos or perlite Shi Shi；

For seed treatment: 2-5000g/100kg seed, preferably 3-1000g/100kg seed；

For soil or processing used for ponds: 0.1-10000g/ha, preferably 1-1000g/ha.

It is fresh-keeping for postharvest fruit and vegetable, 200-2000 times of liquid can be diluted, is leached after soaking fruit.

Above-mentioned dosage is only general exemplary dose, and those skilled in the art can be according to practical feelings when reality is applied Condition and needs adjust rate of application especially in accordance with the seriousness of the property and germ of plant to be processed or crop.

Fluorine thiazole pyrrole ethyl ketone of the invention combines/combined administration with chitosan.Including separating, sequentially or simultaneously applying fluorine Thiazole pyrrole ethyl ketone and chitosan.Preferably, the fluorine thiazole pyrrole ethyl ketone and chitosan group are combined into comprising fluorine thiazole pyrrole ethyl ketone With the form of the composition of chitosan.

Composition of the invention can be based on dosage form, i.e., each substance has mixed in composition, composition at Dividing can also be provided with unit dose form, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with this hair Based on the dosage form of bright offer.

As a further improvement of the present invention, composition pesticide of the invention can be configured to any agent agriculturally allowed Type.

As a further improvement of the present invention, the dosage form of composition pesticide of the invention is suspending agent, seed coat agent, wettable Pulvis, microcapsule suspending agent, coated granule, squeezes out granule, missible oil, microemulsion, aqueous emulsion, effervescent tablet, surpasses water dispersible granules Low capacity liquor, suspoemulsion.

It is living comprising fluorine thiazole pyrrole ethyl ketone and chitosan, filler and/or surface in composition pesticide of the present invention Property agent.

Composition pesticide of the present invention, wherein the content of fluorine thiazole pyrrole ethyl ketone and chitosan accounts for composition pesticide 1%-90%.

The composition pesticide, wherein the content of fluorine thiazole pyrrole ethyl ketone and chitosan accounts for the 5%- of composition pesticide 80%.

The composition pesticide, wherein the content of fluorine thiazole pyrrole ethyl ketone and chitosan accounts for the 10%- of composition pesticide 60%.

According to the present invention, term " filler ", which refers to, can be combined or be combined with reactive compound to make it easier for being administered to The natural or synthetic organic or inorganic compound of object (such as plant, crop or careless class).Therefore, the filler is preferably It is inert, it is acceptable at least to should be agricultural.The filler can be solid or liquid.

The nonactive medium that can be used in the present invention can be used as solid matchmaker either solid is also possible to liquid What dielectric material used has for example: phyteral powdery type (such as soy meal, starch, grain dust, wood powder, bark powder, sawdust, walnut Shell powder, wheat bran, cellulose powder, coconut husk, corncob and tobacco stem particle, the residue etc. after extracting plant essence), paper It opens, sawdust, crushes synthesized polymer body, the clay class (such as kaolin, bentonite, acid china clay etc.), talcum powder of synthetic resin etc. Class.Silica (such as diatomite, silica sand, mica, aqueous silicic acid, calcium silicates), active carbon, natural mineral matter class (float stone, Lv Po Thread stone and zeolite etc.), fire diatomite, sand, (such as polyethylene, polypropylene, the polyvinylidene chloride etc.), chlorination such as plastics medium Chemical fertilizer, the soil of the inorganic mineral powder of potassium, calcium carbonate, calcium phosphate etc., ammonium sulfate, ammonium phosphate, urea, ammonium chloride etc. Fertilizer, these substances can be used alone or two or more is mixed.

It can be used as can selecting in llowing group of materials for liquid media materials'use, such as water, alcohol type (such as first Alcohol, ethyl alcohol, isopropanol, butanol, ethylene glycol etc.), ketone (such as acetone, methyl ethyl ketone, isobutyrone, cyclohexanone Deng), ethers (such as ether, dioxanes, methylcellulose, tetrahydrofuran etc.), aliphatic hydrocarbon class (such as kerosene, Mineral oil etc.), aromatic hydrocarbons class (such as benzene,toluene,xylene, solvent naphtha, alkylnaphthalene, chlorinated aromatic hydrocarbons, chloro rouge Fat hydrocarbon, chlorobenzene, etc.), halogenated hydrocarbon class, amides, sulfone class, mineral and vegetable oil, animal oil etc..

To make the emulsification of effective component compound, dispersion and/or wetting, surfactant can be used for example can be with Enumerate fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol or phenol Phosphate, the aliphatic ester of polyalcohol, alkane aromatic sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonates, the branch shape of macromolecule comb shape are total Polymers, butyl naphthalene sulfonate, alkylaryl sulfonates, sodium alkylsulfosuccinates, grease, fatty alcohol and ethylene oxide condensation The polyacrylates such as object, alkyltaurate, protein hydrolysate.Suitable oligosaccharide object or polymer, such as based on independent Vinyl monomer, acrylic acid, polyoxyethylene and/or polyoxypropylene or itself and such as (polynary) alcohol or (polynary) amine combination.

To make effective component compound dispersion stability, attachment and/or combination, such as xanthan gum, silicic acid can be used Magnalium, gelatin, starch, methyl cellulose, polyvinyl alcohol, polyvinyl acetate and natural phospholipid (such as cephalin and lecithin) with And the adjuvants such as synthetic phospholipid, bentonite, sodium lignin sulfonate.

The wherein optional spent glycol of antifreezing agent, propylene glycol, glycerine, sorbierite.Deflocculant as suspension product The adjuvant such as naphthalene sulfonic acid polymer, polymeric phosphate can be used.

Organic silicon defoamer can be used as defoaming agent.

The colorant that can be used, such as inorganic pigment, such as iron oxide, titanium oxide and Prussian blue；And organic pigment/ Dyestuff: alizarin dyes, azo dyes and metallized phthalocyanine dye；And microelement, such as molysite, manganese salt, boron salt, mantoquita, cobalt Salt, molybdenum salt and zinc salt.

Optionally, also may include other annexing ingredients, for example, protecting colloid, adhesive, thickener, thixotropic agent, bleeding agent, Stabilizer, screening agent.

The reactive compound can be mixed preparation with conventional additives by known way by the preparation of the invention. The conventional additives such as conventional extender and solvent or diluent, emulsifier, dispersing agent, and/or adhesive or fixative, Wetting agent, waterproofing agent, if it is desired, can also include drier and colorant, stabilizer, pigment, defoaming agent, preservative, increasing Thick dose, water and other processing aids.

These compositions not only include can or dusting equipment such as spraying by suitable equipment be suitable for immediately it is to be processed Object, but also including needing the concentration commercial composition being diluted before being applied to object.

Fluorine-containing thiazole pyrrole ethyl ketone of the invention and chitosan can also be applied with other active ingredient combinations, such as Expand activity profile or prevents from forming resistance.Other active constituents such as fungicide, bactericide, attractant, insecticidal Agent, acaricide, nematicide, growth regulator, herbicide, safener, fertilizer or semiochemical etc..

Reactive compound fluorine thiazole pyrrole ethyl ketone and chitosan can be administered simultaneously, or apply respectively or sequential application, separate Sequence when application is on the result of prevention and treatment usually without influence.

Composition pesticide of the invention can be based on dosage form, i.e., each substance has mixed in composition, composition Ingredient can also be provided with unit dose form, mixed in bucket or tank using preceding, be then diluted to required concentration.Wherein preferably with Based on dosage form provided by the invention.

The prevention and treatment of composition pesticide of the invention in the case where reducing reactive compound application total amount, to plant pathogenic fungi And/or improving plant health has improvement active (synergy).

Compared with prior art, the present invention at least has the advantages that

The present invention provides a kind of to have harmful fungoid or bacterium in the case where the application total amount of reactive compound reduces Have and improve active composition pesticide (synergistic composition), to reduce the rate of application of known activity compound and improve its work Property spectrum；And plant health is improved, higher plant fruit yield and/or higher plant or fruit quality can be obtained；And And further reduced the amount of application in agricultural to reactive compound, it further helps in and avoids developing drug resistance to each pesticide.

Specific embodiment

Of the invention for ease of understanding, it is as follows that the present invention enumerates embodiment.Those skilled in the art are it will be clearly understood that the implementation Example is only to aid in the understanding present invention, should not be regarded as a specific limitation of the invention.

Example of formulations

Active constituent, various auxiliary agents and filler etc. are mixed by the proportional components of formula, after ultra-fine pulverizer disintegrating, i.e., Obtain the wettable powder of+0.5% chitosan of 2.5% fluorine thiazole pyrrole ethyl ketone.

Active constituent, various auxiliary agents and filler etc. are mixed by the proportional components of formula, after ultra-fine pulverizer disintegrating, i.e., Obtain the wettable powder of+5% chitosan of 10% fluorine thiazole pyrrole ethyl ketone.

Active constituent, various auxiliary agents and filler etc. will be mixed by the proportional components of formula, after ultra-fine pulverizer disintegrating, Obtain the wettable powder of+10% chitosan of 5% fluorine thiazole pyrrole ethyl ketone.

10% fluorine thiazole pyrrole ethyl ketone+2.5% is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion The dispersible oil-suspending agent of chitosan.

It is mixed evenly the above-mentioned each component to obtain homogeneous phase and gather to get to+0.5% chitin of 1% fluorine thiazole pyrrole ethyl ketone The electrostatic oil agent of sugar.

10% fluorine thiazole pyrrole ethyl ketone more than+1% is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion The suspending agent of fourth glycan.

Said components are ground in the ball mill of stirring, obtain delicate suspensions, are diluted with water to obtain the steady of active component Determine suspension, thus obtains the gel of+0.5% chitosan of 7.5% fluorine thiazole pyrrole ethyl ketone.

25% fluorine thiazole pyrrole ethyl ketone+0.5% is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion The suspension seed-coating agent of chitosan.

In a mixer, by levigate active constituent even spread to the above-mentioned carrier soaked by polyethylene glycol, with this Mode can get the dust-free coated granules agent of+2.5% chitosan of 12.5% fluorine thiazole pyrrole ethyl ketone.

2% fluorine thiazole pyrrole ethyl ketone more than+20% is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion The suspending agent of fourth glycan.

Active component is mixed and ground with auxiliary agent, mixture is soaked with water, which is squeezed out, then in air stream Middle drying obtains the extrusion granule of+4% chitosan of 2% fluorine thiazole pyrrole ethyl ketone.

2.5% fluorine thiazole pyrrole ethyl ketone+10% is obtained after above-mentioned each component is mixed ground and/or high speed shear in proportion The suspension seed-coating agent of chitosan.

Chitosan is dissolved in SOLVESSOTM200, ethoxylated castor oil is added, obtains the oil of chitosan Phase；By fatty alcohol polyoxyethylene ether sulfosuccinic acid monoesters disodium, MODIFIED LIGNOSULPHONATE, fluorine thiazole pyrrole ethyl ketone and water press than Example mixes, and obtains uniformly, obtaining the suspending agent of fluorine thiazole pyrrole ethyl ketone after ground and/or high speed shear；It will be containing chitosan Oil is added in the aqueous suspension agent of fluorine-containing thiazole pyrrole ethyl ketone, obtains the outstanding of+2.5% chitosan of 2.5% fluorine thiazole pyrrole ethyl ketone Emulsion.

Fluorine thiazole pyrrole ethyl ketone, chitosan, dispersing agent, wetting agent, disintegrating agent and filler are mixed according to the formula It is even, by air-flow crushing at wettable powder；It adds a certain amount of water mixing extruding and makes material.10% is obtained after dry screening The water dispersible granules of+2% chitosan of fluorine thiazole pyrrole ethyl ketone.

Said components are mixed in proportion, and grinds, crush, are prepared into+0.5% chitosan of 1% fluorine thiazole pyrrole ethyl ketone Wettable powder.

+ 20% chitosan of 16 80% fluorine thiazole pyrrole ethyl ketone of embodiment

Fluorine thiazole pyrrole ethyl ketone 80%

Chitosan 20%

Fluorine thiazole pyrrole ethyl ketone and chitosan are proportionally uniformly mixed.

Proportion in above embodiments is weight percentage.

Biological test example:

After the compound mixing of different cultivars, three kinds of type of action: summation action, synergistic effect and antagonism are typically exhibited Effect.But it is specially which kind of effect, it is unpredictable, only just it can know that by many experiments.Compound synergic is formulated well, by In significantly improving practical control efficiency, the usage amount of active constituent is reduced, to greatly delay the generation of resistance.

One, virulence is tested:

The toxicity index of each medicament and the co-toxicity coefficient (CTC value) of mixture are calculated according to Sun Yunpei's Method, when CTC≤80, then Composition shows antagonism, and when 80<CTC<120, then composition shows summation action, when CTC>=120, then composition Show synergistic effect.

Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100

Percentage composition+B medicament toxicity index * the mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture The percentage composition of middle B

Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI) * 100

Phytopathogen used in the test of greenhouse virulence and host plant are as shown in the table.

Host plant	Disease title	Genic organisms
			Tomato	Tomato late blight	Phytophthora infestans
Grape	Downy mildew of garpe	The raw uniaxial mould of grape
			Cucumber	Cucumber downy mildew	Cuba artificial downy mildew
Tobacco	Wildfire	Prairie fire germ
			Cucumber	Powdery mildew of cucumber	Melon monofilament shell powdery mildew
Rice	Rice blast	Piricularia oryzae
			Capsicum	Pepper anthracnose	Capsicum colletotrichum

Test 1: the toxicity test of phytophthora infestans

Using inhibition mycelial growth rate method: experiment target is designated as phytophthora infestans.

Fluorine thiazole pyrrole ethyl ketone and chitosan are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The fluorine thiazole pyrrole ethyl ketone of different ratio and chitosan series of concentrations compound solution are made and are contained with same method Malicious culture medium.2 days phytophthora infestans will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, it will with transfer needle Fungus block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times. After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony ten Word, which intersects, surveys two diameters, represents bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 1 is the virulence test result of composition of the invention to phytophthora infestans.

Table 1

As known from Table 1, the combination of fluorine thiazole pyrrole ethyl ketone and chitosan can prevent tomato late blight, in fluorine thiazole pyrrole ethyl ketone When in the range of being 50:1-1:50 with the weight proportion of chitosan, co-toxicity coefficient is all larger than 120, both illustrates in this model Combination in enclosing shows as synergistic effect.

Test 2: the toxicity test of Cuba artificial downy mildew

Using inhibition mycelial growth rate method: experiment target is designated as Cuba artificial downy mildew.

Fluorine thiazole pyrrole ethyl ketone and chitosan are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The fluorine thiazole pyrrole ethyl ketone of different ratio and chitosan series of concentrations compound solution are made and are contained with same method Malicious culture medium.2 days Cuba artificial downy mildews will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, use transfer needle Fungus block is moved to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture in 25 DEG C of incubators, every processing repeats 4 It is secondary.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony Two diameters are surveyed in right-angled intersection, represent bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 2 is the virulence test result of composition of the invention to Cuba artificial downy mildew.

Table 2

As known from Table 2, the combination of fluorine thiazole pyrrole ethyl ketone and chitosan can prevent and treat cucumber downy mildew, in fluorine thiazole pyrrole ethyl ketone When in the range of being 50:1-1:50 with the weight proportion of chitosan, co-toxicity coefficient is all larger than 120, both illustrates in this model Combination in enclosing shows as synergistic effect.

Test 3: the toxicity test of the raw uniaxial mould of grape

Using inhibition mycelial growth rate method: experiment target is designated as the raw uniaxial mould of grape.

Fluorine thiazole pyrrole ethyl ketone and chitosan are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The fluorine thiazole pyrrole ethyl ketone of different ratio and chitosan series of concentrations compound solution are made and are contained with same method Malicious culture medium.By the raw uniaxial mould of the grape for cultivating 2 days, fungus block is broken into colony edge with the punch of diameter 5mm, with inoculation Fungus block is moved to the toxic PDA culture medium center being configured in advance by needle, is subsequently placed in culture in 25 DEG C of incubators, and every processing repeats 4 times.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium It falls right-angled intersection and surveys two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 3 is composition of the invention uniaxial mould virulence test result raw to grape.

Table 3

As known from Table 3, the combination of fluorine thiazole pyrrole ethyl ketone and chitosan can prevent and treat the raw uniaxial mould of grape, in fluorine thiazole pyrrole When the weight proportion of ethyl ketone and chitosan is in the range of 50:1-1:50, co-toxicity coefficient is all larger than 120, illustrates the two at this Combination in a range shows as synergistic effect.

Test 4: the toxicity test of tobacco smoke pollution

Using inhibition mycelial growth rate method: experiment target is designated as tobacco smoke pollution.

Fluorine thiazole pyrrole ethyl ketone and chitosan are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The fluorine thiazole pyrrole ethyl ketone of different ratio and chitosan series of concentrations compound solution are made and are contained with same method Malicious culture medium.2 days prairie fire germs will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3 After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.Table 4 It is the virulence test result of composition of the invention to tobacco smoke pollution.

Table 4

As known from Table 4, the combination of fluorine thiazole pyrrole ethyl ketone and chitosan can prevent and treat tobacco smoke pollution, in fluorine thiazole pyrrole second When the weight proportion of ketone and chitosan is in the range of 50:1-1:50, co-toxicity coefficient is all larger than 120, illustrates the two at this Combination in range shows as synergistic effect.

Test 5: the toxicity test of melon monofilament shell powdery mildew

Using inhibition mycelial growth rate method: experiment target is designated as melon monofilament shell powdery mildew.

Fluorine thiazole pyrrole ethyl ketone and chitosan are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The fluorine thiazole pyrrole ethyl ketone of different ratio and chitosan series of concentrations compound solution are made and are contained with same method Malicious culture medium.2 days melon monofilament shell powdery mildews will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with connecing Fungus block is moved to the toxic PDA culture medium center being configured in advance by kind needle, is subsequently placed in culture in 25 DEG C of incubators, every processing weight It is 4 times multiple.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each Two diameters are surveyed in bacterium colony right-angled intersection, represent bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 5 is the virulence test result of composition of the invention to melon monofilament shell powdery mildew.

Table 5

As known from Table 5, the combination of fluorine thiazole pyrrole ethyl ketone and chitosan can prevent and treat melon monofilament shell powdery mildew, in fluorine thiazole When the weight proportion of pyrrole ethyl ketone and chitosan is in the range of 50:1-1:50, co-toxicity coefficient is all larger than 120, illustrates that the two exists Combination within the scope of this shows as synergistic effect.

Test 6: the toxicity test of piricularia oryzae

Using inhibition mycelial growth rate method: experiment target is designated as piricularia oryzae.

Fluorine thiazole pyrrole ethyl ketone and chitosan are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The fluorine thiazole pyrrole ethyl ketone of different ratio and chitosan series of concentrations compound solution are made and are contained with same method Malicious culture medium.2 days piricularia oryzae will be cultivated, break into fungus block in colony edge with the punch of diameter 5mm, with transfer needle by bacterium Block moves to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture, every processing in 25 DEG C of incubators and is repeated 4 times.3 After it, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony cross Intersect and survey two diameters, bacterium colony size is represented with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 6 is the virulence test result of composition of the invention to piricularia oryzae.

Table 6

As known from Table 6, the combination of fluorine thiazole pyrrole ethyl ketone and chitosan can prevent and treat piricularia oryzae, in fluorine thiazole pyrrole ethyl ketone and When the weight proportion of chitosan is in the range of 50:1-1:50, co-toxicity coefficient is all larger than 120, illustrates the two in this range Interior combination shows as synergistic effect.

Test 7: the toxicity test of capsicum colletotrichum

Using inhibition mycelial growth rate method: experiment target is designated as capsicum colletotrichum.

Fluorine thiazole pyrrole ethyl ketone and chitosan are used into acetone solution respectively, then matched with 0.1% Tween-80 aqueous solution dilution The medical fluid of series of concentrations is made, draws 6mL respectively in superclean bench to the conical flask of sterilizing, 50 DEG C or so of horse is added Bell potato glucose agar medium (PDA) 54mL, pours into the plate of 4 diameter 9cm after shaking up, containing for 4 respective concentrations is made Malicious culture medium；The fluorine thiazole pyrrole ethyl ketone of different ratio and chitosan series of concentrations compound solution are made and are contained with same method Malicious culture medium.2 days capsicum colletotrichums will be cultivated, breaks into fungus block in colony edge with the punch of diameter 5mm, use transfer needle Fungus block is moved to the toxic PDA culture medium center being configured in advance, is subsequently placed in culture in 25 DEG C of incubators, every processing repeats 4 It is secondary.After 3 days, each processing colony diameter cm is measured using crossing method slide calliper rule, correction is found out and inhibits percentage.Each bacterium colony Two diameters are surveyed in right-angled intersection, represent bacterium colony size with its average.Then bacterium colony growth inhibition ratio is found out as the following formula:

Then concentration EC50 in inhibiting is calculated with least square method, then calculates co-toxicity coefficient (CTC) according to Sun Yunpei's Method.

Table 7 is the virulence test result of composition of the invention to capsicum colletotrichum.

Table 7

As known from Table 7, the combination of fluorine thiazole pyrrole ethyl ketone and chitosan can prevent and treat capsicum colletotrichum, in fluorine thiazole pyrrole second When the weight proportion of ketone and chitosan is in the range of 50:1-1:50, co-toxicity coefficient is all larger than 120, illustrates the two at this Combination in range shows as synergistic effect.

Two, plant growth regulating is tested

Test 1 wheat seed

Wheat seed is handled in batch processors.Chemicals to be processed is applied to the center of processor.Starting Processor simultaneously slowly applies chemicals to be maximally distributed on seed.It is mixed by selecting enough incorporation times to control Close intensity.After suitable mixing terminates, stops processor and the seed of processing is fitted into paper bag, for divulging information and doing Dry seed.Compound is with following rate of application (every kg seed) application.After processing and dry seed, by seed suitably to measure It is sowed in field.Selected experimental design is that random block designs, per treatment to be repeated 3 times.In the death of measurement in after planting 71 days The number of plant.The results are shown in Table 8.

Table 8

As known from Table 8, the combination of fluorine thiazole pyrrole ethyl ketone and chitosan shows association for improving the health of wheat seed With the effect of synergy.

Claims (14)

1. a kind of composition pesticide, it is characterised in that: the composition pesticide contains active constituent fluorine thiazole pyrrole ethyl ketone and chitin The weight ratio of glycan, the fluorine thiazole pyrrole ethyl ketone and chitosan be 50:1-1:50, preferably 40:1-1:40, further preferably 30:1-1:30, still more preferably 25:1-1:25, more preferable 20:1-1:20, more preferable 15:1-1:15, more preferable 10:1-1: 10, more preferable 5:1-1:5.

2. composition pesticide according to claim 1, it is characterised in that: the matter of the fluorine thiazole pyrrole ethyl ketone and chitosan The sum of amount accounts for the 5%-90%, preferably 10%-80%, more preferable 20%-60% of the pesticide combination amount of substance.

3. composition pesticide according to claim 1, it is characterised in that: the composition pesticide, dosage form be suspending agent, Seed coat agent, water dispersible granules, microcapsule suspending agent, coated granule, squeezes out granule, missible oil, microemulsion, water at wettable powder Emulsion, effervescent tablet, ultra low volume liquids or suspoemulsion.

4. composition pesticide according to claim 1, it is characterised in that: the composition pesticide also include filler and/ Or surfactant.

5. a kind of prevention and treatment plant pathogenic fungi and/or the method for improving plant health, which is characterized in that by claim 1 institute The composition pesticide stated act on plant pathogen and/or its environment or plant, plant part, plant propagation material or with In plant organ, soil or cultivation medium, material or the space grown afterwards.

6. a kind of prevention and treatment plant pathogenic fungi and/or the method for improving plant health, which is characterized in that will be in claim 1 The fluorine thiazole pyrrole ethyl ketone and chitosan be administered simultaneously or respectively application or sequential application.

7. a kind of prevention and treatment plant pathogenic fungi and/or the method for improving plant health, which is characterized in that infected it in plant It is preceding or after infecting by composition pesticide described in claim 1 act on plant pathogen and/or its environment or plant, In plant part, plant propagation material or the plant organ then grown, soil or cultivation medium, material or space.

8. a kind of prevention and treatment plant pathogenic fungi and/or the method for improving plant health, which is characterized in that expection is grown plant Seed prior to seeding and/or vernalization after with composition pesticide described in claim 1 processing.

9. it is a kind of protect seed method, which is characterized in that the method includes make seed prior to seeding and/or vernalization after with Fluorine thiazole pyrrole ethyl ketone described in claim 1 and chitosan contact.

10. composition pesticide described in claim 1 is sick on Cereal, veterinary antibiotics, ornamental plant and grapevine for preventing and treating The purposes of pathogenic fungi.

11. the purposes that composition pesticide described in claim 1 is used to improve plant health.

12. the phytopathy that composition pesticide described in claim 1 is used to handle seed to protect seed that seed is prevented to be carried Fungal pathogens invasion and/or the purposes for improving plant health.

13. the purposes that composition pesticide described in claim 1 is used to protect the fruits and vegetables after harvest.

14. composition pesticide described in claim 1 is for protecting stock to infect in storage period from plant pathogenic fungi Purposes.