CN114668017A - Application of sterilization composition containing fentrazazole-ethyl and hymexazol in reducing or preventing crop diseases - Google Patents
Application of sterilization composition containing fentrazazole-ethyl and hymexazol in reducing or preventing crop diseases Download PDFInfo
- Publication number
- CN114668017A CN114668017A CN202210201223.9A CN202210201223A CN114668017A CN 114668017 A CN114668017 A CN 114668017A CN 202210201223 A CN202210201223 A CN 202210201223A CN 114668017 A CN114668017 A CN 114668017A
- Authority
- CN
- China
- Prior art keywords
- fusarium
- hymexazol
- solani
- fentrazamide
- composition containing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to application of a sterilization composition containing fentrazamide and hymexazol in reducing or preventing crop infection caused by toxin formed by Fusarium oxysporum neck rot root rot transformation type bacteria (Fusarium oxysporum f.sp.radialis-lycopersici), Fusarium solani (Pyrenochaeta lycopersici Schneider et Ger lach), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum) and Fusarium solani (Fusarium solani).
Description
The application is a divisional application with the application number of 2018102300210, the application date of 2018, 3 and 20, and the invention is named as the application of the sterilization composition containing the fentrazazole-pyrazoethyl and the hymexazol in reducing or preventing crop diseases.
Technical Field
The invention relates to a novel application of a sterilization composition containing fentrazamide and hymexazol in reducing or preventing the crop from being infected by toxin formed by Fusarium oxysporum neck rot root rot transformation type bacteria (Fusarium oxysporum f.sp.radialis-lycopersici), Fusarium solani (Pyrenochaeta lycopersici Schneider et Ger lach), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum) and Fusarium solani (Fusarium solani).
Background
In order to reduce or prevent the infection of crops by Fusarium oxysporum c.f.sp.radialis-lycopersici, chaetomium lycopersici Schneider et gene lack, Fusarium graminearum, Fusarium moniliforme, Fusarium solani (Fusarium solani) pathogens, the following measures are mainly used:
cultivation of cultivars which are not susceptible to pathogenic infection by Fusarium oxysporum (Fusarium oxysporum f.sp. radius-lycopersici), Echinospora solani (Pyrenochaeta lycopersici Schneider et Ger lack), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium protienatum), Fusarium solani (Fusarium solani);
-suitable rotation; the crop rotation is carried out for more than 2 years on the seriously ill field,
after harvesting, timely removing the field disease residues and fallen leaves, carrying out centralized treatment, and carrying out deep ploughing and stubble cleaning.
Storage conditions which prevent the development of the pathogenic bacteria Fusarium oxysporum C.sp.radius-lysopersici, Fusarium solani (Fusarium oxysporum f.sp.radiatum), Fusarium solani (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), and Fusarium solani (Fusarium solani);
-enhancing fertilizer and water management; promoting the growth of crops and preventing the late premature senility of the crops;
-pharmacological control.
However, these purely preventive measures are not satisfactory and the efficacy is not reliable, especially when the prevailing climatic conditions favor pathogenic fungal infestation.
The present invention aims to provide a use of a fungicidal composition containing fentrazamide and hymexazol for reducing or preventing crop diseases, and provides a composition and a method for reducing or preventing crop diseases from being infected by Fusarium oxysporum f.sp.radiatum-lycopersici, chaetomium lycopersicum solani Schneider et Ger lach, Fusarium graminearum, Fusarium candidum, Fusarium moniliforme, Fusarium solani.
The invention surprisingly discovers that the sterilization composition containing the fentrazamide and the hymexazol is excellent in reducing or preventing the crop from being infected by the toxin formed by the pathogenic bacteria of Fusarium oxysporum (solanum oxysporum f.sp.radialis-lycopersici), Fusarium solani (Pyrenochaeta lycopersici Schneider et gelach), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), and Fusarium solani (Fusarium solani).
Tetrazolepin (Picarbtrazox), test code NF-171; CAS: 500207-04-5; tert-butyl [6- [ [ [ [ (z) - (1-methyl-1H-tetrazol-5-yl) (phenyl) methylidene ] amino ] oxy ] methyl ] pyridin-2-yl ] carbamate; the molecular structural formula is:
the tetrazolopyridine ester is oxime ether bactericide developed by Nippon Caoda company, and has good control effect on downy mildew and epidemic diseases. Tetrazolium imidate is known from cn02817805.
The invention provides application of a composition containing fentrazamide and hymexazol in reducing or preventing the infection of crops by toxins formed by Fusarium oxysporum tomato neck rot root rot transformation type bacteria (Fusarium oxysporum f.sp.radialis-lycopersici), chaetomium lycopersicum solani Schneider et Ger lach, Fusarium graminearum (Fusarium graminearum), Fusarium moniliforme (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), and Fusarium solani (Fusarium solani).
Use of a combination of fentrazaethyl and hymexazol for reducing or preventing infestation of crops by toxins formed by the pathogenic bacteria Fusarium oxysporum f.sp.radialis-lycopersici, chaetomium lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach) in a ratio by weight of fentrazaethyl to hymexazol of 50:1-1:50, more preferably 40:1-1:40, more preferably 35:1-1:35, more preferably 30:1-1:30, more preferably 25:1-1:25, more preferably 20:1-1: 20, more preferably 15:1 to 1: 15.
When the composition of the fentrazamide and the hymexazol is used for reducing or preventing the toxin infection of crops caused by fusarium graminearum, fusarium candidum, fusarium moniliforme and fusarium solani, the weight ratio of the fentrazamide to the hymexazol is preferably 1:15-1:50, preferably 1:15-1:40, more preferably 1:15-1:35, more preferably 1:15-1:30, and more preferably 1:15-1: 25.
The invention also provides a sterilization composition containing the fentrazazole-ethyl and the hymexazol, which comprises the fentrazole-ethyl and the hymexazol as active ingredients, a filling agent and/or a surfactant.
In the sterilization composition containing the fentrazazole-ethyl and the hymexazol, the fentrazole-ethyl and the hymexazol account for 1% -90%, preferably 1% -80%, more preferably 1% -70%, more preferably 1% -60%, more preferably 1% -50%, and more preferably 5% -50% of the total weight of the sterilization composition.
The sterilization composition containing the fentrazazole-ethyl and the hymexazol can be prepared into any agriculturally-allowable dosage form. The bactericidal composition is in the dosage form of a suspending agent, a seed treatment dry powder agent, a seed treatment dispersible powder agent, a seed treatment microcapsule suspending agent, a seed treatment suspending agent, a suspension emulsion, a wettable powder, a water dispersible granule, a microcapsule suspending agent, a coating granule, an extrusion granule, missible oil, a microemulsion, an emulsion in water, an effervescent tablet and an ultra-low volume liquid.
The sterilization composition containing the fentrazazole-ethyl and the hymexazol can be applied without dilution or diluted by water.
The invention provides a novel application of a sterilization composition containing fentrazamide and hymexazol in reducing or preventing crops from being infected by Fusarium oxysporum f.sp.radialis-lycopersici, chaetomium lycopersicum Schneider et Ger lach, Fusarium graminearum, Fusarium moniliforme and Fusarium solani pathogenic bacteria.
The novel application of the sterilization composition containing the fentrazamide and the hymexazol in reducing or preventing the crop from being infected by the toxin formed by Fusarium oxysporum tomato neck rot root rot transformation type bacteria (Fusarium oxysporum f.sp.radialis-lycopersici), chaetomium lycopersicum solani Schneider et gelich, Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), and Fusarium solani (Fusarium solani) pathogenic bacteria.
The sterilization composition containing the fentrazamide and the hymexazol is applied to a place needing to be controlled to reduce or prevent the infection of soil or cultivation media by toxins formed by Fusarium oxysporum tomato neck rot transformation type bacteria (Fusarium oxysporum f.sp.radius-lycopersici), chaetomium solani Schneider et Ger lach, Fusarium graminearum (Fusarium graminearum), Fusarium moniliforme (Fusarium proliferatum) and Fusarium solani (Fusarium solani) pathogenic bacteria.
The sterilization composition containing the fentrazamide and the hymexazol is used for treating seeds to reduce or prevent the seeds from being infected by toxins formed by Fusarium oxysporum f.sp.radialis-lycopersici, Fusarium solani Schneider et gelach, Fusarium graminearum, Fusarium moniliforme and Fusarium solani pathogenic bacteria.
The invention provides a method for reducing or preventing the infection of crops by toxins formed by pathogenic bacteria of Fusarium oxysporum (Fusarium oxysporum f.sp. radius-lycopersici), Fusarium solani (Pyrenochaeta lycopersici Schneider et Ger lach), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), Fusarium solani (Fusarium solani), the pathogenic bacteria and/or their environment, or plants, plant propagation materials and plant organs, soils, materials or spaces, storage materials grown subsequently are treated by the bactericidal composition containing the tetrazolium urethane and the hymexazol.
A method for reducing or preventing the infestation of crops by toxins formed by Fusarium oxysporum f.sp.radialis-lycopersici, Fusarium solani Schneider et Ger lach, Fusarium graminearum, Fusarium candidum, Fusarium moniliforme, Fusarium solani and Fusarium solani, by treating the plant, plant propagation material and plant organs, soils, materials or spaces, and objects grown subsequently with a fungicidal composition comprising fentrazamide and hymexazol in a manner such as seed treatment, foliar application, stem application, drench, drip, pour, spray, dusting, scattering, fuming or smoking.
A method for reducing or preventing infestation of crops by toxins formed by Fusarium oxysporum F.sp.radialis-lycopersici, Fusarium solani (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), Fusarium solani (Fusarium solani) pathogens by a fungicidal composition comprising fentrazamide and hymexazol acting on the plant or plant part.
A method for reducing or preventing infestation of crops by toxins formed by the pathogenic bacteria Fusarium oxysporum f.sp.radialis-lycopersici, Fusarium solani (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), Fusarium solani (Fusarium solani), by the action of a fungicidal composition comprising fentrazamide and hymexazol on plant propagation materials and plant organs which grow out subsequently.
A method for reducing or preventing infestation of crops by toxins formed by Fusarium oxysporum F.sp.radialis-lycopersici, Fusarium solani (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), Fusarium solani (Fusarium solani) pathogens by a fungicidal composition comprising fentrazamide and hymexazol acting on the soil or cultivation medium.
The present invention provides a composition for reducing or preventing infection of a crop by a toxin formed by a Fusarium oxysporum-tomato root rot transformant (Fusarium oxysporum f.sp., radicis-lycopersici), Fusarium solani (Pyrenochaeta lycopersici Schneider et Ger lach), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), and Fusarium solani (Fusarium solani) pathogen.
The present invention surprisingly found that fungicidal compositions comprising pyriftalid and hymexazol perform well in reducing or preventing the infestation of crops by toxins formed by Fusarium oxysporum c.f.sp.radialis-lycopersici, chaetomium lycopersicum solani Schneider et gelach, Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), and Fusarium solani (Fusarium solani) pathogenic bacteria.
Detailed Description
Tomato root rot is a crop disease, and mainly damages the stem base and root of crops. The pathogenic bacteria of Fusarium oxysporum, Fusarium oxysporum f.sp.radialis-lycopersici, Fusarium solani (Fusarium graminearum), Fusarium moniliforme (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatorum), Fusarium moniliforme (Fusarium proliferatum), and Fusarium solani (Fusarium solani) are the main pathogenic bacteria causing root rot of tomato. Pathogenic bacteria live through the winter in soil with conidiophores and mycelia along with diseased roots, or in compost which is not fully decomposed, and a large number of conidia are generated in the next year, spread through rainwater or irrigation water and insect propagation, invade from wounds at roots or stem bases, and damage the roots and the roots.
The invention surprisingly discovers that the sterilization composition containing the fentrazamide and the hymexazol is excellent in reducing or preventing the crop from being infected by the toxin formed by Fusarium oxysporum f.sp.radialis-lycopersici, Fusarium solani Schneider et gelch, Fusarium graminearum, Fusarium moniliforme and Fusarium moniliforme.
The present invention provides a fungicidal composition for reducing or preventing infection of crops by toxins formed by the pathogenic bacteria of Fusarium oxysporum, Lycopersis-lycopersici, Oxycosporium lycopersicum (Pyrenochaeta-lycopersici Schneider et Ger lack), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), and Fusarium solani (Fusarium solani).
The invention provides application of a sterilization composition containing fentrazamide and hymexazol in reducing or preventing crops from being infected by Fusarium oxysporum neck rot root rot transformation type bacteria (Fusarium oxysporum f.sp.radialis-lycopersici), chaetomium lycopersicum solani Schneider et gelach, Fusarium graminearum, Fusarium moniliforme and Fusarium solani pathogenic bacteria.
Fenpyrazoxamine (Picarbitrazox), test code NF-171; CAS: 500207-04-5; tert-butyl [6- [ [ [ [ (z) - (1-methyl-1H-tetrazol-5-yl) (phenyl) methylidene ] amino ] oxy ] methyl ] pyridin-2-yl ] carbamate; the molecular structural formula is:
the tetrazolyl pyritinoid is oxime ether bactericide developed by Nippon Caoda company and has better control effect on downy mildew and epidemic diseases. Tetrazolium imidate is known from cn02817805. x.
Use of a fungicidal composition comprising fentrazamide and hymexazol for reducing or preventing infection of crops by toxins formed by Fusarium oxysporum f.sp.radialis-lycopersici and chaetomium solani Schneider et Ger lac pathogenic bacteria, wherein the weight ratio of fentrazamide to hymexazol is 50:1-1:50, more preferably 40:1-1:40, more preferably 35:1-1:35, more preferably 30:1-1:30, more preferably 25:1-1:25, more preferably 20:1-1:20, more preferably 15:1 to 1: 15.
When the bactericidal composition containing the fentrazamide and the hymexazol is used for reducing or preventing the toxin infection of crops caused by Fusarium oxysporum f.sp.radicis-lycopersici and the Fusarium oxysporum Schneider et Ger lach, the weight ratio of the fentrazamide to the hymexazol can be 50:1,49:1,48:1,47:1,46:1,45:1,44:1,43:1,42:1,41:1,40:1,39:1,38:1,37:1,36:1,35:1,34:1,33:1,32:1,31:1,30:1,29:1,28:1,27:1,26:1,25:1,24:1,23:1,22:1,21:1, 16:1, 13:1, 16:1, 11:1,10:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1.5:1,1:1,1:1.5,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13,1:14,1:15,1:16,1:17,1:18,1:19,1:20,1:21,1:22,1:23,1:24,1:25,1:26,1:27,1:28,1:29,1:30,1:31,1:32,1:33,1:34,1:35,1:36,1:37,1:38,1:39,1:40,1:41,1:42,1:43,1:44,1:45,1:46,1:47,1:48,1:49,1:50.
When the bactericidal composition containing the fentrazamide and the hymexazol is used for reducing or preventing the toxin infection of crops caused by pathogenic bacteria of fusarium graminearum (fusarium graminearum), fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium proliferatum) and fusarium solani (fusarium solani), the weight ratio of the fentrazamide to the hymexazol is preferably 1:15-1:50, preferably 1:15-1:40, more preferably 1:15-1:35, more preferably 1:15-1:30, and more preferably 1:15-1: 25.
When the bactericidal composition containing the fentrazamide and the hymexazol is used for reducing or preventing the toxin infection of crops caused by pathogenic bacteria of fusarium graminearum (fusarium graminearum), fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium proliferatum) and fusarium solani (fusarium solani), the weight ratio of the fentrazamide to the hymexazol can be as follows:
1:15,1:16,1:17,1:18,1:19,1:20,1:21,1:22,1:23,1:24,1:25,1:26,1:27,1:28,1:29,1:30,1:31,1:32,1:33,1:34,1:35,1:36,1:37,1:38,1:39,1:40,1:41,1:42,1:43,1:44,1:45,1:46,1:47,1:48,1:49,1:50。
the invention also provides a sterilization composition containing the fentrazazole-ethyl and the hymexazol, which comprises the fentrazole-ethyl and the hymexazol active compound, a filling agent and/or a surfactant.
In the sterilization composition containing the fentrazamide and the hymexazol, the fentrazamide and the hymexazol account for 1% -90%, preferably 1% -80%, more preferably 1% -70%, more preferably 1% -60%, more preferably 1% -50%, and more preferably 5% -50% of the total weight of the sterilization composition.
A fungicidal composition containing fentrazazole-pyrimethanil and hymexazol can be applied directly, in the form of its formulation or in the form of use prepared therefrom. Conventional formulations are, for example, suspensions, dry seed treatment powders, dispersible seed treatment powders, suspension of seed treatment microcapsules, suspension of seed treatment suspensions, suspoemulsions, wettable powders, water dispersible granules, microcapsule suspensions, granules, emulsifiable concentrates, microemulsions, aqueous emulsions, effervescent tablets, ultra-low volume liquids.
In each formulation form, the best possible distribution of the active compounds fenpyrazamine and hymexazol to be used according to the invention and also of the fillers and/or surfactants should be ensured.
The formulations of the invention can be prepared by mixing the active ingredients fentrazamide and hymexazol with fillers and/or surfactants in a known manner, it being possible for further conventional additives, such as siccatives and colorants, stabilizers, pigments, defoamers, preservatives, thickeners, etc., to be added.
According to the present invention, the term "filler" refers to a natural or synthetic organic or inorganic compound that can be combined or associated with an active compound to make it easier to apply to a subject (e.g. plants, crops or grasses). Thus, the filler is preferably inert, at least should be agriculturally acceptable. The filler may be solid or liquid.
Liquid fillers are typically: water, alcohols (e.g., methanol, ethanol, isopropanol, butanol, ethylene glycol, etc.), ketones (e.g., acetone, methyl ethyl ketone, diisobutyl ketone, cyclohexanone, etc.), ethers (e.g., diethyl ether, dioxane, methyl cellulose, tetrahydrofuran, etc.), aliphatic hydrocarbons (e.g., kerosene, mineral oil, etc.), aromatic hydrocarbons (e.g., benzene, toluene, xylene, mineral spirits, alkylnaphthalenes, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, chlorobenzene, etc.), halogenated hydrocarbons, amides, sulfones, dimethyl sulfoxide, mineral and vegetable oils, animal oils, etc.
Solid fillers are typically: examples of the inorganic filler include vegetable powders (for example, particles of soybean powder, starch, cereal powder, wood powder, bark powder, saw dust, walnut shell powder, bran, cellulose powder, coconut shell, corn cob and tobacco stalk, and residues after extraction of plant essence), synthetic polymers such as paper, saw dust and ground synthetic resins, clays (for example, kaolin, bentonite and acid china clay), and talc powders. Silica (for example, diatomaceous earth, silica sand, mica, hydrous silicic acid, calcium silicate), activated carbon, natural minerals (for example, pumice, attapulgite, zeolite, etc.), calcined diatomaceous earth, sand, plastic media (for example, polyethylene, polypropylene, polyvinylidene chloride, etc.), inorganic mineral powders such as potassium chloride, calcium carbonate, calcium phosphate, etc., chemical fertilizers such as ammonium sulfate, ammonium phosphate, urea, ammonium chloride, etc., and soil fertilizers, and these may be used alone or in combination of 2 or more.
Examples of the surfactant that can be used for emulsifying, dispersing, solubilizing and/or wetting the active ingredient compound include polyacrylic acid salts such as fatty alcohol polyoxyethylene ethers, polyoxyethylene alkyl aryl ethers, polyoxyethylene higher fatty acid esters, phosphoric acid esters of polyoxyethylene alcohols or phenols, fatty acid esters of polyhydric alcohols, alkylaryl sulfonic acids, naphthalenesulfonic acid polymers, lignosulfonates, branched polymers of high molecular combs, butylnaphthalenesulfonates, alkylaryl sulfonates, sodium alkylsulfosuccinates, fats and oils, condensates of fatty alcohols with ethylene oxide, and alkyltaurates, and protein hydrolysates. Suitable oligosaccharides or polymers are based, for example, on ethylene monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with, for example, (poly) alcohols or (poly) amines.
Tackifiers such as carboxymethylcellulose and natural and synthetic polymers in the form of powders, granules or latices, such as gum arabic, polyvinyl alcohol and polyvinyl acetate, or else natural phospholipids, such as cephalins and lecithins, and synthetic phospholipids, can also be used in the formulations. Other additives are mineral and vegetable oils.
It is possible to use colorants such as inorganic pigments, for example iron oxide, titanium oxide and Prussian blue, and organic dyes, such as alizarin dyes, azo dyes or metallic phthalocyanine dyes, and trace nutrients, such as salts of iron, manganese, boron, ketones, cobalt, molybdenum and zinc.
Disintegrants which may be used are selected from: one or more of bentonite, urea, ammonium sulfate, aluminum chloride, citric acid, succinic acid and sodium bicarbonate.
Stabilizers which may be used are selected from: one of sodium citrate and resorcinol.
Antifreeze agents which may be used are selected from: one or more of ethylene glycol, propylene glycol, glycerol and urea.
The defoaming agent is selected from: silicone oil, Silicone Compound, C10-20 Saturated fatty acid compound, C8-10One or more of fatty alcohol compounds.
Optionally, further additional components, such as protective colloids, binders, thickeners, thixotropic agents, penetrating agents, stabilizers, masking agents, can also be included.
The bactericidal composition can also be mixed with other medicaments with weeding, insecticidal or bactericidal properties, particularly bactericides. If fentrazamide and hymexazol are used in combination with other fungicides, they are preferably used in combination with one or two other fungicides.
A fungicidal composition containing fentrazazole-pyrimethanil and hymexazol can be applied in combination with the following fungicides:
acylalanines, such as benalaxyl (benalaxyl), metalaxyl (metalaxyl), methylfuroamide (ofarace), oxadixyl (oxadixyl);
Amine derivatives, such as 4-dodecyl-2, 6-dimethylmorpholine (aldimorph), dodine (dodine), dodemorph (dodemorph), fenpropimorph (fenpropimorph), fenpropidin (fenpropidine), biguanide salts (guazatine), iminoctadine acetate (iminoctadine), spiroxamine (spiroxamine), tridemorph (tridemorph);
anilinopyrimidines, such as pyrimethanil, mepanipyrim or cyprodinil;
antibiotics, such as cycloheximide (cycloheximide), griseofulvin (griseofulvin), kasugamycin (kasugamycin), polymalemycin (natamycin), polyoxin (polyoxin) or streptomycin (streptomycin);
azoles, such as bitertanol (biterthanol), bromuconazole (bromoconazole), cyproconazole (cyproconazole), difenoconazole (difenoconazole), dinitonazole (dinitroconazol), fenbuconazole (fenbuconazole), fluquinconazole (fluquinconazole), flusilazole (flusilazole), hexaconazole (hexaconazole), imazalil (imazalil), myclobutanil (myclobutanil), penconazole (penconazole), propiconazole (propiconazole), prochloraz (prochloraz), prothioconazole (prothioconazole), tebuconazole (tebuconazole), triadimefon (triafol), triadimenol (triabendazole), triflumizole (triflumizole), triticonazole (triticonazole), 4- (4-methyl-5, 4-5-methyl-5-4-fluorophenyl) - [1, 6, 4, 6-methyl-triazole ] (1, 5, 4-5, 6-methyl-triazole [1, 6, 4, 5-methyl-triazole ];
Dicarboximides, such as iprodione (iprodione), myclozoline (myclozolin), procymidone (procymidone), vinclozolin;
dithiocarbamates, such as ferbam, sodium (nabam), maneb, mancozeb, metam, metiram, propineb, polycarbamate, thiram, ziram, zineb;
-heterocyclic compounds such as trichlorfon (anilazine), benomyl (benomyl), boscalid (boscalid), carbendazim (carbendazim), carboxin (carboxin), carboxin (oxaboxoxin), cyazofamid (cyazofamid), dazomet (dazomet), dithianon (dithianon), famoxadone (famoxadone), fenamidone (fenamidone), isopimadol (fenarimol), fuberidazole (furaizole), flutolanil (flutolanil), furamethozole (furametpyr), benomyl (mepronil), nuarimol), probenazole (propazofamid), proquinazine (proquinazine), praziquantel (pyroquil), quinacridone (quinacril), thiabendazole (thiabendazole), thifluazid (prothioconazole), praziquantel (pyrad), praziquantel (pyroquilon), quinacril (quinacril), thiabendazole (thiabendazole), thiabendazole (thiabendazole);
Copper fungicides, such as bordeaux mixture, copper acetate, copper oxychloride (copperoxochloride), basic copper sulfate;
phenylpyrroles, such as fenpiclonil or fluoroxacillin (fluoroxonil);
sulfenic acid derivatives, such as captafol (captafol), captan (captan), dichlofluanid (dichlofluanid), folpet (folpet), tolylfluanid (tolyfluoride);
cinnamide and similar compounds, such as dimethomorph, flurbiprofen or flumorph.
Strobilurins (strobilurins), such as azoxystrobin (azoxystrobin), dimoxystrobin (dimoxystrobin), fluoxastrobin (fluoxastrobin), fenpyrad (kresoxim-methyl), metominostrobin (metominobin), orysastrobin (orysastrobin), picoxystrobin (picoxystrobin), or trifloxystrobin (trifloxystrobin);
other fungicides, such as thiadiazoline (acibenzolar-S-methyl), benthiavalicarb (benthiavalicarb), chlorocyclopropanamide (carpropamid), chlorothalonil (chlorothalonil), cyflufenamid (cyflufenamid), cymoxanil (cymoxanil), diclomezine (diclomezine), diclocyanamide (diclocymet), diethofencarb (diethofencarb), edifenphos (edifenphos), ethaboxam (ethaboxam), fenhexamid (fenhexamid), fenhexamid (fenoxanil), pyrimethanzone (ferimzone), fluazinam (fluazinam), fosetyl-aluminium (foseyl-alum), iprovalicarb, hexachlorobenzene (hexazemazone), chlorothalonil (penfluniprol), penfluniumuron (penfluniprol), penfluniprol (penfluniprol), penoxsulam (penoxsulam), penoxsulam (thiophanate), penoxsulam), chlorothalonil (thiophanate), penoxsulide (thiophanate), penoxsulide (thiophanate) and penoxsulide (thiophanate) are (e) are) and the like.
The sterilization composition containing the fentrazazole-polyurethane and the hymexazol can be mainly prepared in a preparation form, namely, all substances in the composition are mixed; the ingredients of the composition may also be provided in a single dose form, mixed in a tub or tank prior to use, and then diluted to the desired concentration. Preferably, the formulation provided by the invention is used as the main ingredient.
The application of the sterilization composition containing the fentrazamide and the hymexazol in reducing or preventing the infection of plants, plant propagation materials and plant organs which grow out later by toxins formed by Fusarium oxysporum neck rot transformation type bacteria (Fusarium oxysporum f.sp.radialis-lycopersici), chaetomium lycopersicum (Pyrenochaeta decoridier et Ger lach), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum) and Fusarium solani (Fusarium solani) is disclosed.
The application of the sterilization composition containing the fentrazamide and the hymexazol to the site needing to be controlled reduces or prevents the infection of soil or cultivation media by toxins formed by Fusarium oxysporum f.sp.radialis-lycopersici, chaetomium lycopersicum solani Schneider et gelach, Fusarium graminearum, Fusarium candidum, Fusarium moniliforme (Fusarium proliferatum) and Fusarium solani (Fusarium solani) pathogenic bacteria.
The sterilization composition containing the fentrazamide and the hymexazol is used for treating seeds to reduce or prevent the seeds from being infected by toxins formed by Fusarium oxysporum neck rot root rot transformation type bacteria (Fusarium oxysporum f.sp.radialis-lycopersici), Fusarium solani (Pyrenochaeta lycopersici Schneider et Ger lach), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium protienatum), and Fusarium solani (Fusarium solani).
The invention provides a method for reducing or preventing the reduction or prevention of the infestation of crops by toxins formed by the pathogenic bacteria Fusarium oxysporum F.sp.radialis-lycopersici, Echinospora solani (Pyrenochaeta lycopersici Schneider et Ger lack), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), Fusarium solani (Fusarium solani), by treating the plants and/or their environment, or the plants, plant propagation material and plant organs, soil, material or space, storage pathogenic bacteria which grow out later with said bactericidal composition containing polyurethane tetrazolium and hymexazol.
A method for reducing or preventing the infestation of crops by toxins formed by Fusarium oxysporum f.sp.radialis-lycopersici, Fusarium solani Schneider et Ger lach, Fusarium graminearum, Fusarium candidum, Fusarium moniliforme, Fusarium solani and Fusarium solani, by treating the plant, plant propagation material and plant organs, soils, materials or spaces, and objects grown subsequently with a fungicidal composition comprising fentrazamide and hymexazol in a manner such as seed treatment, foliar application, stem application, drench, drip, pour, spray, dusting, scattering, fuming or smoking.
A method for reducing or preventing infestation of crops by toxins formed by Fusarium oxysporum F.sp.radialis-lycopersici, Fusarium solani (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), Fusarium solani (Fusarium solani) pathogens by a fungicidal composition comprising fentrazamide and hymexazol acting on the plant or plant part.
A method for reducing or preventing the infestation of crops by toxins formed by the pathogenic bacteria Fusarium oxysporum f.sp.radialis-lycopersici, Fusarium solani (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatorum), Fusarium solani (Fusarium solani), by fungicidal compositions containing fentrazamide and hymexazol on plant propagation materials and plant organs which grow out subsequently.
A method for reducing or preventing the infection of crops by toxins formed by the pathogenic bacteria Fusarium oxysporum f.sp.radialis-lycopersici, Fusarium solani (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), Fusarium moniliforme (Fusarium solani), and Fusarium solani (Fusarium solani) by the action of a fungicidal composition containing fentrazamide and hymexazol on the soil or cultivation medium.
The application time, the number of applications and the application rate used in each case must be adapted to the prevailing conditions and must be determined by the person skilled in the art for each individual case.
Treatment with a germicidal composition containing pyriftalid and hymexazol can be protective and therapeutic. Either before or after infestation by harmful fungi. Preferably, it is carried out as close as possible to the time of infestation, i.e. before or after the infestation as close as possible to the time point of infestation.
The sterilization composition containing the fentrazazole-ethyl and the hymexazol can be used as a foliar fungicide in crop protection, can also be used as a fungicide for seed dressing and soil fungicide, and can also be used as a preservative for stored materials after picking.
The sterilization composition containing the fenpyrazamine and the hymexazol can treat all crops. "crop" means all plants and plant populations such as desirable and undesirable wild plants, cultivars, and plant varieties (whether or not protected by the plant variety or plant cultivar rights). Cultivated plants and plant varieties may be plants obtained by conventional propagation and cultivation methods, which may be supplemented or supplemented by one or more biotechnological methods, for example using dihaploids, protoplast fusion, random and directed mutations, molecular or genetic markers, or using bioengineering and genetic engineering methods. Plant parts are understood to mean all parts and organs of plants above and below the ground, such as shoots, leaves, flowers and roots, such as leaves, needles, stems, branches, flowers, fruit bodies, fruits and seeds, and also roots, bulbs and rhizomes. Also plants and vegetative and generative propagation material, for example cuttings, bulbs, rhizomes, runners and seeds, belong to the plant part.
Suitable crops include mainly field crops, such as maize, soybean, cotton, canola oil seeds, such as Brassica napus (e.g. canola), turnip (Brassica rapa), mustard (e.g. mustard (mustard)), rice, wheat, sugar beets, sugar cane, oats, rye, barley, millet, triticale, flax, grapevine and fruit or vegetable crops of various plant classes, such as rosaceous fruits (e.g. pomefruit, such as apples and pears), but also stone fruits, such as apricots, cherries, almonds and peaches, berries, such as strawberries), theaceae (riciosaidae sp.), jugaceae, betulaceae, Anacardiaceae (Anacardiaceae sp.), Fagaceae (Fagaceae sp.), Moraceae (Moraceae sp.), lignaceae (Oleaceae sp.), Fagaceae (arabiaceae sp.), moreaceae sp.), malaceae (macaque (e), macaque (planaceae), Musaceae (plantaceae (bananas), Musaceae (plantaginea), Musaceae (plantagine), Musaceae (plantaginea), Musaceae (bananas), plantaginea (plantagine (bananas), etc.) Rubiaceae (Rubiaceae sp.) (e.g., coffee), Theaceae (Theaceae sp.), firmianaceae (sterculiceae sp.), Rutaceae (Rutaceae sp.) (e.g., lemon, orange, and grapefruit); solanaceae (solanaceae sp.) (e.g. tomatoes, potatoes, peppers, eggplants), Liliaceae (Liliaceae sp.), compositaceae (Compositiae sp.) (e.g. lettuce, artichoke and chicory-including root chicory (root chicory), endive (endive) or common chicory), Umbelliferae (Umbelliferae sp.) (e.g. carrots, parsley, celery and celeries), Cucurbitaceae (Cucurbitaceae sp.) (e.g. cucumbers-including pickled cucumbers (pickling cuumber), squash, watermelons, cucurbits and melons), Alliaceae (Alliaceae sp.) (e.g. onions and leeks), Cruciferae sp. (e.g. white cabbage, red cabbage, broccoli, cabbage, cauliflower, broccoli, brussels sprouts, cabbage, parsley, radish, bean, lentils, beans (bean), lentils, beans, and beans, and beans, and beans, and beans, and beans, and beans, and beans, and beans, and beans, and beans, and beans, Chenopodiaceae (Chenopodiaceae sp.) (e.g., fodder beet, spinach sweet (spinach beet), spinach, beetroot), Malvaceae (Malvaceae) (e.g., okra), asparagiceae (Asparagaceae) (e.g., Asparagus); horticultural and forest crops; an ornamental plant; and genetically modified homologues of these crops. Solanaceae (solanaceae), for example, tomato, potato, pepper, eggplant, are preferred.
The term "plant propagation material" is understood to mean all plant parts having the ability to propagate, such as seeds, which can be used for propagating the latter, and also vegetative materials, such as cuttings or tubers. Thus, plant parts as used herein include plant propagation material. Mention may be made, for example, of seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated plants and useful plants to be inhibited after germination or after emergence from the soil. The young plants can be protected prior to transplantation by a total or partial treatment by dipping.
Preferred plant propagation material of the present invention is a seed. The sterilization composition containing the fentrazazole-ethyl and the hymexazol is also particularly suitable for treating seeds. Most of the crop damage caused by harmful fungi is caused by the attack of the seeds during storage or after sowing and during or after germination of the plants. Plants are particularly sensitive to roots and shoots during the growing period and can cause death of the plant even if there is little damage. This stage is particularly critical, since it is very important to protect the seeds and the germinating plants by using suitable compositions.
The invention also relates in particular to reducing or preventing the infestation of seeds and germinating plants by toxins formed by the pathogenic bacteria of Fusarium oxysporum (Fusarium oxysporum f.sp.radici-lycopersici), Fusarium solani (Fusarium graminearum et Ger lach), Fusarium graminearum (Fusarium graminearum), Fusarium moniliforme (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), and Fusarium solani (Fusarium solani) by treating the seeds with the fungicidal composition comprising fentrazamide and hymexazol according to the invention. The method makes it unnecessary or at least significantly possible to additionally apply crop protection agents after sowing or after germination of the plants. On the other hand, the amount of active compound used is optimized with the fungicidal composition according to the invention in order to provide maximum protection of the seeds and the germinating plants from attack by phytopathogenic fungi, without the plants themselves being harmed by the active compound used.
The germicidal composition containing fentrazamide and hymexazol of the present invention is suitably treated in a sufficiently stable state so that the treatment does not cause any damage to the seeds. In general, the treatment of the seeds can be performed at any point in time between picking and sowing. Commonly used seeds are isolated from the plant and separated from the cob, husk, stem, cuticle, hair or pulp. Thus, for example, seeds that have been picked, cleaned and dried to a moisture content of less than 15% may be used. Alternatively, seeds which have been dried, for example, by treatment with water, and then dried again can also be used.
Examples of the method of treating the seed include a method of diluting a liquid or solid chemical or directly immersing the seed in a liquid solution without dilution to allow the chemical to permeate the seed, a method of mixing a solid chemical or liquid chemical with the seed to coat the seed to attach the chemical to the surface of the seed, and a method of spraying the chemical to the vicinity of the seed while planting.
The sterilization composition containing the fentrazazole-ethyl and the hymexazol is particularly favorable for treating seeds, in particular the seeds of tomatoes, hot peppers, potatoes, tobaccos, rapes, cottons, soybeans, corns, peanuts, alfalfa, medlar, cucumbers, barley, wheat and rice. Seeds of tomato, pepper, potato, tobacco, medlar, alfalfa are preferred.
The control of phytopathogenic fungi that harm the plants after germination is mainly performed by treating the soil and the aerial parts of the plants with a germicidal composition containing pyriftalid and hymexazol according to the present invention.
The fungicidal composition containing the fentrazamide and the hymexazol can also be used for treating soil to reduce or prevent the infection of crops by toxins formed by Fusarium oxysporum f.sp.radialis-lycopersici, Fusarium solani et al, Fusarium graminearum, Fusarium moniliforme, and Fusarium solani pathogens.
Methods of applying agents to soil include methods of spraying a "germicidal composition" onto soil, methods of mixing a "germicidal composition" with soil, and methods of irrigating a "germicidal composition" into soil.
The sterilization composition containing the fentrazamide and the hymexazol can be used for treating plants or plant aboveground parts to reduce or prevent the infection of crops by toxins formed by Fusarium oxysporum neck rot root rot transformation type bacteria (Fusarium oxysporum f.sp.radius-lycopersici), chaetomium solani (Pyrenochaeta lycopersici Schneider et Ger lac), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), and Fusarium solani (Fusarium solani) pathogenic bacteria. When applied to plants or parts of plants above ground, it is used in the form of an aqueous spray. Application is preferably by spraying. The spray is sprayed over the entire above-ground portion of the plant or only over individual plant parts. The choice of the individual plant parts to which the spray liquid is to be applied depends on the plant species and its stage of development.
The sterilization composition containing the fentrazamide and the hymexazol can also be used for treating stored materials to reduce or prevent the infection of toxins formed by Fusarium oxysporum neck rot root rot transformation type bacteria (Fusarium oxysporum f.sp.radialis-lycopersici), Fusarium solani (Pyrenochaeta lycopersici Schneider et gelach), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium protienatum), and Fusarium solani (Fusarium solani) pathogenic bacteria.
According to the invention, the term "stock" is understood to mean natural substances and processed forms thereof of plant or animal origin which have been derived from the natural life cycle and for which long-term preservation is desired. Stocks of plant origin, for example plants or parts thereof, such as stems, leaves, tubers, seeds, fruits or grains, can be protected in the freshly harvested state or in processed form, such as (pre) drying, wetting, crushing, grinding, pressing or baking. Or wood, coarse wood forms such as construction timber, utility poles and fences; or in finished form, such as furniture or articles made of wood. The stock of animal origin is hide, leather, hair, etc. The compositions according to the invention can prevent fungal or bacterial attack such as corrosion, discoloration or mildew during storage. "stock" is preferably understood to mean natural substances of plant origin and processed forms thereof.
The sterilization composition containing the fentrazazole polyurethane and the hymexazol can be processed by different processing methods:
-spraying a liquid comprising the fungicidal composition onto the aerial parts of the plants;
-dusting, incorporating granules or powders in the soil, spraying around said plants and, in the case of tree injection or painting;
-coating or film coating the seeds of the plants.
The present invention provides a method for reducing or preventing the infection of a crop by a toxin formed by a Fusarium oxysporum F.sp.radialis-lycopersici, Fusarium solani Schneider et Ger lach, Fusarium graminearum, Fusarium moniliforme, Fusarium solani, which may be a method for treating, preventing or eradicating the infection.
Typically for leaf treatment: 0.1 to 10000g/ha, preferably 10 to 1000 g/ha, more preferably 50 to 500 g/ha; for dipping or instillation administration, the dosage may even be reduced, particularly when an inert substrate such as asbestos or perlite is applied;
-for seed treatment: 2-5000g/100kg of seeds, preferably 3-1000g/100kg of seeds;
-applying a treatment to the soil or water surface: 0.1 to 10000g/ha, preferably 1 to 1000 g/ha.
The above-mentioned dosages are only typical exemplary dosages, and the person skilled in the art will adjust the application rate in the actual application according to the actual circumstances and needs, in particular according to the nature of the plants or crops to be treated and the severity of the germs.
Indoor toxicity assay
Test one: virulence determination of Fusarium oxysporum tomato neck rot root rot transformation (Fusarium oxysporum f.sp.radialis-lycopersici)
Adopting a method for inhibiting the growth rate of hypha:
dissolving fentrazapyrin and hymexazol with acetone respectively, diluting with 0.1% tween-80 water solution to obtain medicinal liquid with series concentration, sucking 6mL into sterilized triangular flask respectively in clean bench, adding 54mL of potato glucose agar culture medium (PDA) at 50 deg.C, shaking, and pouring into 4 dishes with diameter of 9cm to obtain 4 toxic culture media with corresponding concentrations; the toxicity-containing culture medium is prepared by compounding liquid medicines of series concentrations of the fentrazole-pyritinol and the hymexazol in different proportions by the same method. Fusarium oxysporum tomato neck rot root rot transformation type bacteria (Fusarium oxysporum f.sp. radius-lycopersici) cultured for 2 days are punched into bacterial blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are moved to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are placed in an incubator at 25 ℃ for culture, and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Then calculating the concentration EC in the inhibition by using a least square method50Then, the co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.
Table 1: toxicity test results for Fusarium oxysporum tomato neck rot root rot transformation type bacteria (Fusarium oxysporum f.sp.radialis-lycopersici)
As can be seen from table 1, when the combination of pyrazoxate and hymexazol is in the range of the ratio of 50:1 to 1: 50, the co-toxicity coefficient to Fusarium oxysporum c.p.radius-lycopersici transformed bacteria is greater than 120, showing a synergistic effect.
And (2) test II: virulence determination of Achillea lycopersici (Pyrenochaeta lycopersici Schneider et Ger lach)
Adopting a method for inhibiting the growth rate of hypha:
dissolving fentrazapyrin and hymexazol with acetone respectively, diluting with 0.1% tween-80 water solution to obtain medicinal liquid with series concentration, sucking 6mL into sterilized triangular flask respectively in clean bench, adding 54mL of potato glucose agar culture medium (PDA) at 50 deg.C, shaking, and pouring into 4 dishes with diameter of 9cm to obtain 4 toxic culture media with corresponding concentrations; the toxicity-containing culture medium is prepared by compounding liquid medicines of series concentrations of the fentrazole-pyritinol and the hymexazol in different proportions by the same method. A2-day-old culture of Achillea lycopersici (Pyrenochaeta lycopersici Schneider et Ger lach) was performed by forming a block at the edge of the colony using a punch having a diameter of 5mm, transferring the block to the center of a previously prepared toxic PDA medium using an inoculating needle, and culturing the block in an incubator at 25 ℃ 4 times per treatment. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Then calculating the concentration EC in the inhibition by using a least square method50Then, the cotoxicity coefficient (CTC) was calculated by the Sun Yunpei method.
Table 2: results of virulence test on A. lycopersici (Pyrenochaeta lycopersici Schneider et Ger lach)
As can be seen from Table 2, when the combination of pyrazoxate and hymexazol is in the range of the mixture ratio of 50:1-1:50, the co-toxicity coefficient to the acanthosporium lycopersici Schneider et Ger lach is more than 120, and the synergistic effect is shown.
And (3) test III: virulence determination of Fusarium graminearum (Fusarium graminearum)
Adopting a method for inhibiting the growth rate of hypha:
dissolving fentrazapyrin and hymexazol with acetone respectively, diluting with 0.1% tween-80 water solution to obtain medicinal liquid with series concentration, sucking 6mL into sterilized triangular flask respectively in clean bench, adding 54mL of potato glucose agar culture medium (PDA) at 50 deg.C, shaking, and pouring into 4 dishes with diameter of 9cm to obtain 4 toxic culture media with corresponding concentrations; the toxicity-containing culture medium is prepared by compounding liquid medicines of series concentrations of the fentrazole-pyritinol and the hymexazol in different proportions by the same method. Fusarium graminearum (Fusarium graminearum) cultured for 2 days was cut into clumps at the edges of the colony using a punch of 5mm diameter, the clumps were transferred to the center of a previously prepared PDA medium containing toxins using an inoculating needle, and then cultured in an incubator at 25 ℃ 4 times per treatment. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Then calculating the concentration EC in the inhibition by using a least square method50Then, the cotoxicity coefficient (CTC) was calculated by the Sun Yunpei method.
Table 3: virulence test results against Fusarium graminearum (Fusarium graminearum)
As can be seen from table 3, when the combination of fentrazamide and hymexazol is in the range of the ratio of 1:15 to 1:50, the co-toxicity coefficient to fusarium graminearum (fusarium graminearum) is greater than 120, and the synergistic effect is shown.
And (4) testing four: virulence assay for Fusarium candidum (Fusarium moniliforme)
Adopting a method for inhibiting the growth rate of hypha:
dissolving fentrazapyrin and hymexazol with acetone respectively, diluting with 0.1% tween-80 water solution to obtain medicinal liquid with series concentration, sucking 6mL into sterilized triangular flask respectively in clean bench, adding 54mL of potato glucose agar culture medium (PDA) at 50 deg.C, shaking, and pouring into 4 dishes with diameter of 9cm to obtain 4 toxic culture media with corresponding concentrations; the toxicity-containing culture medium is prepared by compounding liquid medicines of series concentrations of the fentrazole-pyritinol and the hymexazol in different proportions by the same method. Fusarium candidum (Fusarium moniliforme) cultured for 2 days was cut into clumps at the edge of the colony using a punch of 5mm diameter, the clumps were transferred to the center of a previously prepared PDA medium containing toxin using an inoculating needle, and then cultured in an incubator at 25 ℃ for 4 replicates per treatment. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Then calculating the concentration EC in the inhibition by using a least square method50Then, the cotoxicity coefficient (CTC) was calculated by the Sun Yunpei method.
Table 4: virulence test results against Fusarium candidum (Fusarium moniliforme)
As can be seen from table 4, when the combination of fentrazamide and hymexazol is in the range of the ratio of 1:15 to 1:50, the co-toxicity coefficient to fusarium candidum (fusarium moniliforme) is greater than 120, and the synergistic effect is shown.
And (5) testing: virulence assay for Fusarium moniliforme (Fusarium proliferatum)
Adopting a method for inhibiting the growth rate of hypha:
dissolving fentrazapyrin and hymexazol with acetone respectively, diluting with 0.1% tween-80 water solution to obtain medicinal liquid with series concentration, sucking 6mL into sterilized triangular flask respectively in clean bench, adding 54mL of potato glucose agar culture medium (PDA) at 50 deg.C, shaking, and pouring into 4 dishes with diameter of 9cm to obtain 4 toxic culture media with corresponding concentrations; the toxicity-containing culture medium is prepared by compounding liquid medicines of series concentrations of the fentrazole-pyritinol and the hymexazol in different proportions by the same method. Fusarium moniliforme (Fusarium proliferatum) cultured for 2 days is punched into bacterial blocks at the edges of the bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are moved to the center of a prepared toxic PDA culture medium by an inoculating needle, and then the bacterial blocks are cultured in an incubator at 25 ℃ and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Then calculating the concentration EC in the inhibition by using a least square method50Then, the co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.
Table 5: virulence test results on Fusarium moniliforme (Fusarium proliferatum)
As can be seen from table 5, when the combination of fentrazamide and hymexazol is in the range of the ratio of 1:15 to 1:50, the co-toxicity coefficient to fusarium moniliforme (fusarium proliferatum) is greater than 120, showing a synergistic effect.
And (6) test six: virulence assay for Fusarium solani (Fusarium solani)
Adopting a method for inhibiting the growth rate of hypha:
dissolving fentrazapyrin and hymexazol with acetone respectively, diluting with 0.1% tween-80 water solution to obtain medicinal liquid with series concentration, sucking 6mL into sterilized triangular flask respectively in clean bench, adding 54mL of potato glucose agar culture medium (PDA) at 50 deg.C, shaking, and pouring into 4 dishes with diameter of 9cm to obtain 4 toxic culture media with corresponding concentrations; the compound liquid medicine with series concentrations of the fentrazamide and the hymexazol in different proportions is prepared into the toxic medium by the same method. Fusarium solani (Fusarium solani) cultured for 2 days is punched into a bacterial block at the edge of the bacterial colony by a punch with the diameter of 5mm, the bacterial block is moved to the center of a prepared toxic PDA culture medium by an inoculating needle, and then the bacterial block is placed in an incubator at 25 ℃ for culture, and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:
Then calculating the concentration EC in the inhibition by using a least square method50Then, the co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei method.
Table 6: virulence test results on Fusarium solani (Fusarium solani)
As can be seen from table 6, when the combination of pyrazoxate and hymexazol is in the range of the ratio of 1:15 to 1:50, the co-toxicity coefficient to fusarium solani (fusarium solani) is greater than 120, and the synergistic effect is shown.
Test for drug efficacy
Test 1 Activity/seed treatment of Fusarium oxysporum C.sp.radialis-Lycoperpersici
The plastic pots were filled with soil infected with Fusarium oxysporum (Fusarium oxysporum f.sp. radius-lysorcici), Fusarium solani (Pyrenochaeta lysobacter et Ger lach), Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium moniliforme (Fusarium proliferatum), Fusarium solani (Fusarium solani), respectively, and the tomato seeds treated with the test compounds were inoculated into the soil and grown in the greenhouse for 20 days.
The infestation by pathogenic fungi from each germinated young plant was observed, and the degree of disease infestation in the "treated sample" was calculated according to the following calculation formula (1).
On the other hand, tomato seeds not treated as above were cultivated in the same manner as described above, and the infestation by pathogenic fungi from each germinated young plant was observed, and the degree of disease infestation in the "untreated sample" was calculated according to the following calculation formula (1).
The efficacy in the "treated sample" was calculated according to the following calculation formula (2) based on the degree of disease infection in the "treated sample" and the degree of disease infection in the "untreated sample".
Calculating formula (1):
pest infestation (%) = (number of young plants infected/total number of young plants) × 100
Calculating formula (2):
efficacy (%) = [1- ("degree of disease infestation in treated-like"/"degree of disease infestation in untreated-like") ] 100
TABLE 1 efficacy on Fusarium oxysporum C.sp.radialis-lycopersici
Table 1 the test results show that the combination of pyrazoxate and hymexazol is excellent in controlling Fusarium oxysporum c.p.radialis-lycopersici.
TABLE 2 efficacy against Achillea lycopersici (Pyrenochaeta lycopersici Schneider et Ger lach)
The results of the tests in Table 2 show that the combination of pyrazoxate and hymexazol is excellent in control of Acrophyta lycopersici (Pyrenochaeta lycopersici Schneider et Ger lach).
TABLE 3 efficacy on Fusarium graminearum (Fusarium graminearum)
The results of the tests in table 3 show that the combination of fentrazamide and hymexazol is excellent in controlling fusarium graminearum.
TABLE 4 efficacy against Fusarium candidum (Fusarium moniliforme)
Table 4 the test results show that the combination of pyrazoxate and hymexazol is excellent in controlling fusarium candidum (fusarium moniliforme).
TABLE 5 efficacy against Fusarium moniliforme (Fusarium proliferatum)
The results of the tests in table 5 show that the combination of fentrazamide and hymexazol is excellent in controlling fusarium moniliforme (fusarium proliferatum).
TABLE 6 efficacy against Fusarium solani (Fusarium solani)
The results of the tests in table 6 show that the combination of pyrazoxate and hymexazol is excellent in controlling fusarium solani (fusarium solani).
Claims (13)
1. The application of the bactericidal composition containing the fentrazamide and the hymexazol in reducing or preventing the toxin infection of crops caused by pathogenic bacteria of Fusarium graminearum, Fusarium moniliforme and Fusarium solani is characterized in that the weight ratio of the fentrazamide to the hymexazol is 1:15-1: 50.
2. The use as claimed in claim 1, wherein the weight ratio of the fentrazamide to the hymexazol is 1:15-1: 40.
3. The use as claimed in claim 1, wherein the weight ratio of the fentrazamide to the hymexazol is 1:15-1: 35.
4. The use as claimed in claim 1, wherein the weight ratio of the fentrazole-pyrimethanil to the hymexazol is 1:15-1: 30.
5. The use as claimed in claim 1, wherein the weight ratio of the fentrazazole-based imidate to the hymexazol is 1:15-1: 25.
6. Use according to claim 1, characterized in that the crop is selected from potato, pepper, eggplant, alfalfa, tomato, pepper, potato, tobacco, rape, cotton, soybean, corn, peanut, wolfberry, cucumber, barley, wheat, rice.
7. The use as claimed in claim 1, wherein the germicidal composition containing the polyurethane tetrazolium and the hymexazol is characterized in that the polyurethane tetrazolium and the hymexazol account for 1-90% of the total weight of the germicidal composition.
8. The use as claimed in claim 7, wherein the germicidal composition containing the polyurethane tetrazolium and the hymexazol is characterized in that the polyurethane tetrazolium and the hymexazol account for 1-60% of the total weight of the germicidal composition.
9. The use as claimed in claim 7, wherein the germicidal composition containing the polyurethane tetrazolium and the hymexazol is characterized in that the polyurethane tetrazolium and the hymexazol account for 5-50% of the total weight of the germicidal composition.
10. The use as claimed in claim 1, wherein the germicidal composition containing fentrazamide and hymexazol is in the dosage form of suspension, dry seed treatment powder, dispersible seed treatment powder, suspension for seed treatment microcapsules, suspension for seed treatment, suspoemulsion, wettable powder, water dispersible granules, suspension for microcapsules, coated granules, extruded granules, emulsifiable concentrate, microemulsion, aqueous emulsion, effervescent tablets, ultra-low volume liquid.
11. A method for reducing or preventing infestation of crops by toxins formed by the pathogenic bacteria fusarium graminearum (fusarium graminearum), fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium proliferatum), fusarium solani (fusarium solani), characterized in that a pathogenic bacterium and/or its environment, or a plant, plant propagation material and plant organs, soils, materials or spaces, storage products which grow out later, is treated with a fungicidal composition according to claim 1.
12. The method according to claim 11, characterized in that the pathogenic bacteria and/or their environment, or the plant, the plant propagation material and the plant organs, soils, materials or spaces, storage products which subsequently grow out are treated with the germicidal composition comprising fentrazamide and hymexazol in the form of seed treatment, foliar application, stem application, drench, drip, pour, spray, mist, dusting, scattering or fuming.
13. The method of claim 11, wherein the treatment is performed before or after infestation by pathogenic bacteria.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210201223.9A CN114668017B (en) | 2018-03-20 | 2018-03-20 | Use of bactericidal composition containing tetrazole picoline and hymexazol in reducing or preventing crop diseases |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810230021.0A CN110301445B (en) | 2018-03-20 | 2018-03-20 | Application of sterilization composition containing fentrazazole-ethyl and hymexazol in reducing or preventing crop diseases |
| CN202210201223.9A CN114668017B (en) | 2018-03-20 | 2018-03-20 | Use of bactericidal composition containing tetrazole picoline and hymexazol in reducing or preventing crop diseases |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810230021.0A Division CN110301445B (en) | 2018-03-20 | 2018-03-20 | Application of sterilization composition containing fentrazazole-ethyl and hymexazol in reducing or preventing crop diseases |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114668017A true CN114668017A (en) | 2022-06-28 |
| CN114668017B CN114668017B (en) | 2023-10-13 |
Family
ID=68073862
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210201223.9A Active CN114668017B (en) | 2018-03-20 | 2018-03-20 | Use of bactericidal composition containing tetrazole picoline and hymexazol in reducing or preventing crop diseases |
| CN201810230021.0A Active CN110301445B (en) | 2018-03-20 | 2018-03-20 | Application of sterilization composition containing fentrazazole-ethyl and hymexazol in reducing or preventing crop diseases |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810230021.0A Active CN110301445B (en) | 2018-03-20 | 2018-03-20 | Application of sterilization composition containing fentrazazole-ethyl and hymexazol in reducing or preventing crop diseases |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN114668017B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220167620A1 (en) * | 2019-04-05 | 2022-06-02 | Mitsui Chemicals Agro, Inc. | Agrochemical composition with enhanced efficacy |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106922707A (en) * | 2015-12-29 | 2017-07-07 | 浙江新农化工股份有限公司 | Bactericidal composition and its preparation and application containing picarbutrazox |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106922709B (en) * | 2015-12-29 | 2022-07-12 | 浙江新农化工股份有限公司 | Composition of picarbratzox and sterol biosynthesis inhibitor bactericide, preparation and application thereof |
| CN106922708A (en) * | 2015-12-29 | 2017-07-07 | 浙江新农化工股份有限公司 | Compositions of the picarbutrazox with succinate dehydrogenase inhibitors series bactericidal agent and its preparation and application |
-
2018
- 2018-03-20 CN CN202210201223.9A patent/CN114668017B/en active Active
- 2018-03-20 CN CN201810230021.0A patent/CN110301445B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106922707A (en) * | 2015-12-29 | 2017-07-07 | 浙江新农化工股份有限公司 | Bactericidal composition and its preparation and application containing picarbutrazox |
Non-Patent Citations (3)
| Title |
|---|
| 李丽娜 等: "四种杀菌剂对玉米穗腐病菌的室内毒力测定" * |
| 王育彪，张果斌，焦建平，高占朋: "7种杀菌剂对马铃薯干腐病菌的抑制及病害防治效果" * |
| 秦莉，李海燕，刘惕若，李德刚，于裴枝: "咪鲜胺和恶霉灵对水稻立枯病菌的室内毒力测定" * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110301445A (en) | 2019-10-08 |
| CN110301445B (en) | 2022-05-13 |
| CN114668017B (en) | 2023-10-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EA018963B1 (en) | Combinations of biological control agents with a nematicidal seed coating | |
| EA023712B1 (en) | Active compound combinations comprising a (thio)carboxamide derivative and a fungicidal compound | |
| TWI617244B (en) | Fungicidal composition and the use thereof | |
| EA017238B1 (en) | Pesticidal combinations | |
| Augusto et al. | Assessing systemicity of peanut fungicides through bioassay of plant tissues with Sclerotium rolfsii | |
| UA126246C2 (en) | Fungicidal compositions | |
| CN114223665B (en) | Use of bactericidal composition for preventing and controlling plant pathogenic fungi | |
| UA77981C2 (en) | Fungicidal compositions based on pyridylmethylbenzamide derivative and valinamide derivative, method for curative or preventive control of phytopathogenic fungi of crops | |
| CN110301445B (en) | Application of sterilization composition containing fentrazazole-ethyl and hymexazol in reducing or preventing crop diseases | |
| CN108260593B (en) | Bactericidal composition | |
| CN114286622A (en) | Antifungal composition containing natamycin and at least one insecticide | |
| CN111109268A (en) | Bactericidal composition containing kresoxim-methyl and tetraconazole | |
| CN114617127B (en) | Pesticide sterilization composition and application thereof | |
| CN109221146B (en) | Use of triflumizole amine for reducing or preventing corn infection by helminthosporium macrostoma | |
| CN110710532B (en) | Application of bactericidal composition containing trifloxystrobin and difenoconazole | |
| CN110710534B (en) | Application of sterilization composition containing fluopyram and mancozeb | |
| CN108522515A (en) | A kind of application of the bactericidal composition containing kresoxim-methyl and tetraconazole | |
| CN110810437B (en) | Application of bactericidal composition containing zinc thiazole and thiediazole copper | |
| CN106982851B (en) | Bactericidal composition | |
| CN106982842B (en) | Bactericidal composition | |
| CN110301450A (en) | Tetrazolium pyrrole urethane is reducing or preventing the purposes in crop disease | |
| CN110710536B (en) | Application of bactericidal composition containing trifloxystrobin and mancozeb | |
| CN107593710B (en) | Bactericidal composition | |
| CN114097802B (en) | Emulsifiable concentrate containing epoxiconazole and isoprothiolane | |
| CN110810427A (en) | Application of bactericidal composition containing zinc thiazole and benziothiazolinone |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |