CN114668017B - Use of bactericidal composition containing tetrazole picoline and hymexazol in reducing or preventing crop diseases - Google Patents
Use of bactericidal composition containing tetrazole picoline and hymexazol in reducing or preventing crop diseases Download PDFInfo
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- CN114668017B CN114668017B CN202210201223.9A CN202210201223A CN114668017B CN 114668017 B CN114668017 B CN 114668017B CN 202210201223 A CN202210201223 A CN 202210201223A CN 114668017 B CN114668017 B CN 114668017B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
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Abstract
The application relates to the use of a bactericidal composition containing tetrazolium picolinate and hymexazol for reducing or preventing crop infestation by toxins formed by fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthocellum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium moniliforme), fusarium moniliforme (fusarium pro-feratum), fusarium solani (fusarium solani).
Description
The application relates to a divisional application with application number of 2018102300210 and application date of 2018, 3 and 20, and the name of the divisional application is 'application of a bactericidal composition containing tetrazolium picolinate and hymexazol in reducing or preventing crop diseases'.
Technical Field
The application relates to a novel application of a bactericidal composition containing tetrazolium picolinate and hymexazol in reducing or preventing crop infection by toxins formed by fusarium oxysporum neck rot root rot transformation bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthocellum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium moniliforme), fusarium moniliforme (fusarium pro-feratum) and fusarium solani (fusarium solani).
Background
In order to reduce or prevent the infestation of crops by fusarium oxysporum tomato neck rot root rot transformant bacteria (Fusarium oxysporum f.sp. radicis-lycopersici), tomato acanthosporium (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium moniliforme (fusarium moniliforme), fusarium solani (fusarium solani) pathogens, the currently used measures are mainly the following:
cultivation of cultivars which are not susceptible to infection by fusarium oxysporum tomato neck rot root rot transformant (Fusarium oxysporum f.sp. radicis-lycopersici), tomato acanthocera (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium moniliforme), fusarium solani (fusarium solani) pathogens;
-suitable rotation; the field with serious disease is subjected to rotation for more than 2 years,
after harvesting, the field disease and residue and fallen leaves are removed in time, the concentrated treatment is carried out, and the deep ploughing and stubble cleaning are carried out.
Storage conditions preventing the development of fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp. radicis-lycopersici), acanthosporium lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium prodiferum), fusarium solani (fusarium solani);
-enhancing fertilizer water management; promoting the growth of crops and preventing early senescence in the later period of the crops;
-pharmaceutical control.
However, these purely preventive measures are not satisfactory and not reliable in effectiveness, especially when the prevailing climatic conditions favour pathogenic fungal infections.
Summary of the inventionthe present invention aims to address the above-mentioned shortcomings by providing a use of a bactericidal composition containing tetrazolium picolinate and hymexazol for reducing or preventing crop disease and to provide a composition and method for reducing or preventing crop infestation by fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), acanthosis lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium grarum), fusarium moniliforme (fusarium moniliforme), fusarium moniliforme (fusarium pro-interference), fusarium solani (fusarium solani) pathogens.
The present invention surprisingly found that a fungicidal composition comprising tetrazolium picolinate and hymexazol is excellent in reducing or preventing crop infestation by fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), acanthosporium lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium prodiferum), fusarium solani (fusarium solani) pathogenic bacteria.
Tetrazolium picornase (picrbutanzox), test code NF-171; CAS:500207-04-5; the chemical formula is [6- [ [ [ [ (z) - (1-methyl-1H-tetrazol-5-yl) (phenyl) methylene ] amino ] oxy ] methyl ] pyridin-2-yl ] carbamic acid tert-butyl ester; the molecular structural formula is:
tetrazolium picolinate is an oxime ether bactericide developed by the company Cauda in Japan, and has good control effect on downy mildew and epidemic disease. Tetrazolium picolinate is known from cn02817805. X.
The invention provides a use of a composition containing tetrazolium picolinate and hymexazol in reducing or preventing crop infection by toxins formed by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), acanthosis lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium moniliforme), fusarium moniliforme (fusarium pro-ferratum), fusarium solani (fusarium solani).
Use of a combination of tetrazolium picolinate and hymexazol in reducing or preventing crop infestation by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), acanthospira lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach) pathogens in a weight ratio of tetrazolium picolinate to hymexazol of 50:1-1:50, more preferably 40:1-1:40, more preferably 35:1-1:35, more preferably 30:1-1:30, more preferably 25:1-1:25, more preferably 20:1-1: 20, more preferably 15:1 to 1:15.
The use of a combination of tetrazolium picornase and hymexazol in reducing or preventing crop infestation by toxins formed by fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium moniliforme), fusarium solani (fusarium solani), preferably the weight ratio of tetrazolium picornase to hymexazol is 1:15-1:50, preferably 1:15-1:40, more preferably 1:15-1:35, more preferably 1:15-1:30, more preferably 1:15-1:25.
The invention also provides a bactericidal composition containing the tetrazole picoline and the hymexazol, which comprises the tetrazole picoline and the hymexazol flexible component, the filler and/or the surfactant.
In the bactericidal composition containing the tetrazole picolide and the hymexazol, the total weight of the bactericidal composition contains 1% -90%, preferably 1% -80%, more preferably 1% -70%, more preferably 1% -60%, more preferably 1% -50%, and more preferably 5% -50%.
The bactericidal composition containing the tetrazole picolide and the hymexazol can be prepared into any agriculturally allowable dosage form. The bactericidal composition is prepared from suspending agents, seed treatment dry powder agents, seed treatment dispersible powder agents, seed treatment microcapsule suspending agents, seed treatment suspending agents, wettable powder agents, water dispersible granules, microcapsule suspending agents, coated granules, extrusion granules, emulsifiable concentrates, microemulsions, aqueous emulsion, effervescent tablets and ultra-low volume liquid agents.
The bactericidal composition containing the tetrazole picolide and the hymexazol can be applied without dilution or can be applied by dilution with water.
The invention provides a novel application of a bactericidal composition containing tetrazolium picolinate and hymexazol in reducing or preventing crop infection by fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthocellum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium moniliforme), fusarium moniliforme (fusarium pro-ferrate) and fusarium solani (fusarium solani) pathogenic bacteria.
The novel application of the bactericidal composition containing tetrazolium picolinate and hymexazol in reducing or preventing crop infection by toxins formed by fusarium oxysporum tomato neck rot root rot transformation bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthocellum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium moniliforme), fusarium moniliforme (fusarium pro-interference) and fusarium solani (fusarium solani).
The use of a bactericidal composition containing tetrazolium picolinate and hymexazol for reducing or preventing infection of soil or cultivation medium by toxins formed by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), fusarium solani (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium moniliforme), fusarium solani (fusarium solani) pathogenic bacteria when applied to a site to be controlled.
The use of a bactericidal composition containing tetrazolium picolinate and hymexazol for treating seeds to reduce or prevent infestation of the seeds by toxins formed by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), acanthosporium lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium proliferatum), fusarium solani (fusarium solani) pathogenic bacteria.
The present invention provides a method for reducing or preventing crop infestation by fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthocellum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium pro-reference), fusarium solani (fusarium solani) pathogenic bacteria by treating pathogenic bacteria and/or their environment, or plants, plant propagation material and subsequently growing plant organs, soil, material or space, storage with said bactericidal composition comprising tetrazolium picolinate and hymexazol.
A method of reducing or preventing crop infestation by fusarium oxysporum tomato neck rot root rot transformant bacteria (Fusarium oxysporum f.sp. radicis-lycopersici), tomato acanthosporium (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium moniliforme (fusarium moniliforme), fusarium solani (fusarium solani) by toxins formed by fusarium solani pathogens, treating pathogenic bacteria and/or their environment, or plant, plant propagation material or plant organs, soil, material or space, stock, etc. with a bactericidal composition containing tetrazolium picolinate and hymexazol in seed treatment, foliar application, stem application, soaking, instillation, pouring, spraying, dusting, scattering or fuming, etc.
A method for reducing or preventing crop infestation by toxins formed by Fusarium oxysporum tomato neck rot root rot transformant (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthosporium (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (Fusarium graminearum), fusarium candidum (Fusarium moniliforme), fusarium moniliforme (Fusarium moniliforme), fusarium solani (Fusarium solani) pathogens, by applying a bactericidal composition containing tetrazolium picolinate and hymexazol to plants or plant parts.
A method for reducing or preventing crop infestation by toxins formed by fusarium oxysporum tomato neck rot root rot transformant bacteria (Fusarium oxysporum f.sp. radicis-lycopersici), tomato acanthosporium (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium moniliforme (fusarium moniliforme), fusarium solani (fusarium solani) by applying a bactericidal composition comprising tetrazolium picolinate and hymexazol to plant propagation material and subsequently growing plant organs.
A method for reducing or preventing crop infestation by Fusarium oxysporum tomato neck rot root rot transformant bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthosporium (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (Fusarium graminearum), fusarium candidum (Fusarium moniliforme), fusarium moniliforme (Fusarium moniliforme), fusarium solani (Fusarium solani) pathogenic bacteria forming toxins, by applying a bactericidal composition containing tetrazolium picolinate and hymexazol to soil or cultivation medium.
The present invention provides a composition for reducing or preventing crop infestation by fusarium oxysporum tomato neck rot root rot transformant (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthocellum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium pro-interference), fusarium solani (fusarium solani) pathogens forming toxins.
The present inventors have surprisingly found that a fungicidal composition comprising tetrazolium picolinate and hymexazol is excellent in reducing or preventing crop infestation by fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp. radicis-lycopersici), acanthosis lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium pro-limosum), fusarium solani (fusarium solani) pathogenic bacteria.
Detailed Description
Tomato root rot is a crop disease that mainly damages the root and stem base of crops. Fusarium oxysporum tomato neck rot root rot transformant (Fusarium oxysporum f.sp. radicis-lycopersici), tomato acanthosis (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium proliferatum), fusarium solani (fusarium solani) pathogens are the main pathogens causing tomato root rot. Pathogenic bacteria overwhelm in soil with the root of disease by means of conidium devices and mycelia, and also overwhelm in insufficiently decomposed compost, and a large amount of conidium is generated next year and spread through rainwater or irrigation water and insect transmission, invade from root or stem base wounds, and is harmful to root and rhizome.
The present invention surprisingly found that a fungicidal composition comprising tetrazolium picolinate and hymexazol is excellent in reducing or preventing crop infestation by fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), acanthosporium lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium prodiferum), fusarium solani (fusarium solani) pathogenic bacteria.
The present invention provides a fungicidal composition for reducing or preventing crop infestation by fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp. radicis-lycopersici), tomato acanthocellum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium pro-interference), fusarium solani (fusarium solani) pathogenic bacteria.
The invention provides a use of a bactericidal composition containing tetrazolium picolinate and hymexazol in reducing or preventing crop infection by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), acanthosis lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium moniliforme), fusarium moniliforme (fusarium pro-feratum) and fusarium solani (fusarium solani) pathogenic bacteria.
Tetrazolium picornase (picrbutanzox), test code NF-171; CAS:500207-04-5; the chemical formula is [6- [ [ [ [ (z) - (1-methyl-1H-tetrazol-5-yl) (phenyl) methylene ] amino ] oxy ] methyl ] pyridin-2-yl ] carbamic acid tert-butyl ester; the molecular structural formula is:
tetrazolium picolinate is an oxime ether bactericide developed by the company Cauda in Japan, and has good control effect on downy mildew and epidemic disease. Tetrazolium picolinate is known from cn02817805. X.
Use of a bactericidal composition containing tetrazolium picolinate and hymexazol in a weight ratio of 50:1 to 1:50, more preferably 40:1 to 1:40, more preferably 35:1 to 1:35, more preferably 30:1 to 1:30, more preferably 25:1 to 1:25, more preferably 20:1 to 1, for reducing or preventing crop infestation by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), and by pathogenic bacteria of the species echinococcus lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach):20, more preferably 15:1 to 1:15.
Use of a fungicidal composition comprising tetrazolium picolinate and hymexazol for reducing or preventing crop infestation by toxins formed by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici) and acanthocellum lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach) pathogenic bacteria, the weight ratio of the tetrazole picoline to the hymexazol can also be that 50:1,49:1,48:1,47:1,46:1,45:1,44:1,43:1,42:1,41:1,40:1,39:1,38:1,37:1,36:1,35:1,34:1,33:1,32:1,31:1,30:1,29:1,28:1,27:1,26:1,25:1,24:1,23:1,22:1,21:1,20:1,19:1,18:1,17:1,16:1,15:1,14:1,13:1,12:1,11:1,10:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1, 1:1, 1:1:5:1, 1:1, 1:1:1, 1:1:1:1, 2:1:1:1, 1:1:1, 2:1:1:1, 1:1:1, 35:1:1:1, 35:1:1, 35:1, 1,35:1, 1,35:1, 1:1, 35:1, 1,35:1, 1:1, 35:1, 1,1:1, 35:1, 1,1:1,1, 1:1:1, 1,1:1,1, 2:1,1, 2:1,1, 21, 21 21 2 a. The invention relates to a method for producing a fibre-reinforced plastic composite.
Use of a bactericidal composition containing tetrazolium picolinate and hymexazol in a weight ratio of preferably 1:15-1:50, preferably 1:15-1:40, more preferably 1:15-1:35, more preferably 1:15-1:30, more preferably 1:15-1:25, for reducing or preventing crop infestation by toxins formed by fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium moniliforme), fusarium moniliforme (fusarium solani) pathogens.
Use of a bactericidal composition containing tetrazolium picornase and hymexazol for reducing or preventing crop infestation by toxins formed by fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium moniliforme), fusarium moniliforme (fusarium proliferatum), fusarium solani (fusarium solani), the weight ratio of tetrazolium picornase to hymexazol may also be:
1:15,1:16,1:17,1:18,1:19,1:20,1:21,1:22,1:23,1:24,1:25,1:26,1:27,1:28,1:29,1:30,1:31,1:32,1:33,1:34,1:35,1:36,1:37,1:38,1:39,1:40,1:41,1:42,1:43,1:44,1:45,1:46,1:47,1:48,1:49,1:50。
the invention also provides a bactericidal composition containing the tetrazole picoline and the hymexazol, which comprises the tetrazole picoline and the hymexazol flexible compound, the filler and/or the surfactant.
In the bactericidal composition containing the tetrazole picoline and the hymexazol, the tetrazole picoline and the hymexazol account for 1% -90%, preferably 1% -80%, more preferably 1% -70%, more preferably 1% -60%, more preferably 1% -50% and more preferably 5% -50% of the total weight of the bactericidal composition.
The bactericidal composition containing tetrazole picolide and hymexazol can be applied directly, in the form of its formulation or in the form of use prepared therefrom. Conventional formulations are, for example, suspensions, seed-treated dry powders, seed-treated dispersible powders, seed-treated microcapsule suspensions, seed-treated suspensions, suspoemulsions, wettable powders, water dispersible granules, microcapsule suspensions, granules, emulsifiable concentrates, microemulsions, aqueous emulsions, effervescent tablets, ultra-low volume liquids.
In each formulation form, the best possible distribution of the active compounds tetrazolium picolinate and hymexazol and of the fillers and/or surfactants used according to the invention is ensured.
The formulations of the present invention may be prepared by mixing the active ingredients tetrazolium picolinate and hymexazol with fillers and/or surfactants in known manner, and may also be supplemented with other conventional additives such as siccatives and colorants, stabilizers, pigments, defoamers, preservatives, thickeners, and the like.
According to the present invention, the term "filler" refers to a natural or synthetic organic or inorganic compound that can be combined or associated with an active compound to make it easier to apply to a subject (e.g., plants, crops or grasses). Thus, the filler is preferably inert, at least should be agriculturally acceptable. The filler may be solid or liquid.
The liquid filler is typically: water, alcohols (e.g., methanol, ethanol, isopropanol, butanol, ethylene glycol, etc.), ketones (e.g., acetone, methyl ethyl ketone, diisobutyl ketone, cyclohexanone, etc.), ethers (e.g., diethyl ether, dioxane, methyl cellulose, tetrahydrofuran, etc.), aliphatic hydrocarbons (e.g., kerosene, mineral oil, etc.), aromatic hydrocarbons (e.g., benzene, toluene, xylene, solvent oil, alkyl naphthalene, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, chlorobenzene, etc.), halogenated hydrocarbons, amides, sulfones, dimethyl sulfoxide, mineral and vegetable oils, animal oils, etc.
The solid filler is typically: plant powder (such as soybean powder, starch, grain powder, wood powder, bark powder, sawdust, walnut shell powder, bran, cellulose powder, coconut shell, corn cob and tobacco stem particles, residues after extraction of plant essence, etc.), paper, sawdust, synthetic polymers of pulverized synthetic resin, clays (such as kaolin, bentonite, acidic porcelain clay, etc.), talc, etc. Silica (e.g., diatomaceous earth, silica sand, mica, hydrous silicic acid, calcium silicate), activated carbon, natural minerals (pumice, attapulgite, zeolite, etc.), burned diatomaceous earth, sand, plastic media, etc. (e.g., polyethylene, polypropylene, polyvinylidene chloride, etc.), inorganic mineral powders of potassium chloride, calcium carbonate, calcium phosphate, etc., chemical fertilizers such as ammonium sulfate, ammonium phosphate, urea, ammonium chloride, etc., and earth fertilizers, which may be used alone or in combination of 2 or more.
Examples of the surfactant that can be used for emulsifying, dispersing, solubilizing, and/or wetting the active ingredient compound include fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid ester, phosphate ester of polyoxyethylene alcohol or phenol, fatty acid ester of polyhydric alcohol, alkylaryl sulfonic acid, naphthalene sulfonic acid polymer, lignin sulfonate, branched copolymer of polymer comb, butylnaphthalene sulfonate, alkylaryl sulfonate, sodium alkylsulfonate, oil and fat, condensate of fatty alcohol and ethylene oxide, polyacrylate such as alkyl taurate, and protein hydrolysate. Suitable oligosaccharides or polymers are, for example, based on ethylene monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with, for example, (poly) alcohols or (poly) amines.
Tackifiers such as carboxymethylcellulose and natural and synthetic polymers in the form of powders, granules or latices, such as gum arabic, polyvinyl alcohol and polyvinyl acetate or natural phospholipids, such as cephalins and lecithins, and synthetic phospholipids, can also be used in the formulations. Other additives are mineral oil and vegetable oil.
Colorants such as inorganic pigments, for example iron oxide, titanium oxide and Prussian blue, and organic dyes, such as alizarin dyes, azo dyes or metal phthalocyanine dyes, and trace nutrients, such as iron, manganese, boron, ketones, cobalt, molybdenum and zinc salts, may be used.
The disintegrants which may be used are selected from: bentonite, urea, ammonium sulfate, aluminum chloride, citric acid, succinic acid and sodium bicarbonate.
Stabilizers which may be used are selected from: sodium citrate, resorcinol.
The antifreeze agents which may be used are selected from: one or more of ethylene glycol, propylene glycol, glycerol and urea.
The defoamer is selected from the group consisting of: silicone oil and silicone compound、C 10-20 Saturated fatty acid compound, C 8-10 One or more of fatty alcohol compounds.
Optionally, other additional components may also be included, such as protective colloids, binders, thickeners, thixotropic agents, penetrating agents, stabilizers, masking agents.
The bactericidal composition of the invention can also be mixed with other medicines with weeding, insecticidal or bactericidal properties, in particular to bactericides. If tetrazole picoline and hymexazol are used in combination with other fungicides, they are preferably used in combination with one or two other fungicides.
A bactericidal composition containing tetrazole picolide and hymexazol can also be applied in combination with the following fungicides:
acyl lactams such as benalaxyl (benalaxyl), metalaxyl (metalaxyl), furalamide (ofurace), oxadixyl (oxadixyl);
Amine derivatives, such as 4-dodecyl-2, 6-dimethylmorpholine (aldimorph), dodine (dodemorph), molinate (fenpropimorph), fenpropidin (fenpropidin), biguanide salts (guazatine), biguanide octaacetate (iminostadine), spiroxamine (spiroxamine), and crellin (tridemorph);
anilinopyrimidines, such as pyrimethanil (pyrimethanil), mepanipyrim (mepanipyrim) or cyprodinil (cyprodinil);
antibiotics, such as cycloheximide, griseofulvin, kasugamycin, polymamycin, polyoxin or streptomycin;
-an azole group, which is selected from the group consisting of, for example bitertanol, furfuryl alcohol (bromoconazole), cyproconazole (cyproconazole), difenoconazole (difenoconazole), diniconazole (diniconazole), fenbuconazole (fenbuconazole), fluquinconazole (fluquinconazole), flusilazole (flusilazole), hexaconazole (hexaconazole), carbendazim (imazalil), myclobutanil (myclobutanil), penconazole (penconazole), propiconazole (propiconazole), prochloraz (prochloraz), prothioconazole (prothioconazole), tebuconazole), triadimefon (triazodimethon), triazofenozole (triazofenol), fluquinconazole (fluquinconazole), 5-chloro-7- (4-methyl-piperidine) -1, 6-1, 4-trifluoro-1, 6-2-trifluoro-1, 4-6-trifluoro-1, 6-1-trifluoro-2-1-6-trifluoro-2-1-3-trifluoro-2-3-methyl-2-phenyl-2-methyl-2-benzoxazole;
Dicarboximides, such as iprodione (iprodione), tolfenpyrad (myclobulin), procymidone (procymidone), metrafenone (vinclozolin);
dithiocarbamates, such as ferbam, mancozeb, manacre, metiram, propineb (propineb), polytriamate (polycarbobam), thiram, ziram (ziram), zineb (zineb);
heterocyclic compounds, such as dichlofenamate (anilazine), benomyl (benomyl), boscalid (boscalid), carbendazim (carboxin), carboxin (oxacarboxin), cyazofamid (azofamid), dazomet (dazomet), dithianon (dithianon), oxazolone, imidazolone (fenamidone), isopyrazaol (fenpyroxim), fuberidazole (fuberizole), fluoroamide (fluvalanil), furazolidone (furameter), propiconazole (mevalonazole), flubenomyl (nuarimol), thiabendazole (profenozole), propioquinoline (prozid), praziquantel (pyrazox), fluquindox (pyroquin), fluquindox (fluben), flubenazol (flubenazol), flubenazol (thiazide), flubenazol (methyl), flubenazol (trifloxystrobin), flubenmid (flubenazol (trifloxystrobin) and flubenmid (flubenmid);
Copper fungicides, such as bordeaux mixture, copper acetate, copper king (coppereoxide), basic copper sulphate;
phenylpyrroles, such as fenpiclonil (fenpiclonil) or fluoxastrobin (fluxion);
sulfoacid derivatives, such as captan (captafol), captan (captan), dichlofluanid (dichlofluanid), folpet (folpet), tolylfluanid (tolylfluanid);
cinnamamide and similar compounds such as dimethomorph (dimethomorph), fluorobiphenyl (fluretover) or flumorph (flumoph).
Strobilurins (strobilurins) such as azoxystrobin (azoxystrobin), dimoxystrobin (dimoxystrobin), fluoxastrobin (fluoxastrobin), picoline (kresoxim-methyl), phenoxymycetin (metominotrbin), orysastrobin (orysastrobin), picoxystrobin (picoxystrobin), or trifloxystrobin (trifloxystrobin);
other fungicides, such as thiabendazole (acibenzolar-S-methyl), benthiavalicarb (benthiavalicarb), chlorocyclopropylamide (carboplatin), chlorothalonil (chlorothalonil), cyflufenamide (cyflufenamid), cymoxanil (cymoxanil), pyridalyl (dichlormid), dicyclopentadienyl (dicyclopentadien), diethofencarb (diethofencarb), pyrimethanil (edifenphos), ethaboxam (ethaboxam), cycloxaprop (fenhexamid), fenhexamid (fenhexamid), azophos (azophos), fluazinam (fluazinam), hexachlor-ethyl (fluazinam), hexachlorphenamine (methyl), penfluben (methyl), tetrachlorazone (methyl), and tetrachlorazone (methyl).
The bactericidal composition containing the tetrazole picolide and the hymexazol can take the preparation form as a main part, namely, all substances in the composition are mixed; the components of the composition may also be provided in a single dose form, mixed in a tank or canister prior to use, and then diluted to the desired concentration. Wherein the formulation forms provided by the present invention are preferred.
The use of a bactericidal composition containing tetrazolium picolinate and hymexazol for reducing or preventing infestation of plants, plant propagation material and subsequently growing plant organs by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), fusarium solani (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium candidum (fusarium moniforme), fusarium moniliforme (fusarium prodiferum), fusarium solani (fusarium solani) pathogenic bacteria.
The use of a bactericidal composition containing tetrazolium picolinate and hymexazol for reducing or preventing infection of soil or cultivation medium by toxins formed by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), fusarium solani (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium moniliforme), fusarium solani (fusarium solani) pathogenic bacteria when applied to a site to be controlled.
The use of a bactericidal composition containing tetrazolium picolinate and hymexazol for treating seeds to reduce or prevent infestation of the seeds by toxins formed by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), acanthosporium lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium candidum (fusarium moniliforme), fusarium moniliforme (fusarium proliferatum), fusarium solani (fusarium solani) pathogenic bacteria.
The present invention provides a method for reducing or preventing the infestation of crops by fusarium oxysporum tomato neck rot root rot transformant (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthocellulose (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (fusarium graminearum), fusarium moniliforme (fusarium pro-feratum), fusarium solani (fusarium solani) pathogenic bacteria, treating pathogenic bacteria and/or their environment, or plants, plant propagation material and subsequently growing plant organs, soil, material or space, and storage with said bactericidal composition containing tetrazolium picolide and hymexazol.
A method of reducing or preventing crop infestation by fusarium oxysporum tomato neck rot root rot transformant bacteria (Fusarium oxysporum f.sp. radicis-lycopersici), tomato acanthosporium (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium moniliforme (fusarium moniliforme), fusarium solani (fusarium solani) by toxins formed by fusarium solani pathogens, treating pathogenic bacteria and/or their environment, or plant, plant propagation material or plant organs, soil, material or space, stock, etc. with a bactericidal composition containing tetrazolium picolinate and hymexazol in seed treatment, foliar application, stem application, soaking, instillation, pouring, spraying, dusting, scattering or fuming, etc.
A method for reducing or preventing crop infestation by toxins formed by Fusarium oxysporum tomato neck rot root rot transformant (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthosporium (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (Fusarium graminearum), fusarium candidum (Fusarium moniliforme), fusarium moniliforme (Fusarium moniliforme), fusarium solani (Fusarium solani) pathogens, by applying a bactericidal composition containing tetrazolium picolinate and hymexazol to plants or plant parts.
A method for reducing or preventing crop infestation by toxins formed by fusarium oxysporum tomato neck rot root rot transformant bacteria (Fusarium oxysporum f.sp. radicis-lycopersici), tomato acanthosporium (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium moniliforme (fusarium moniliforme), fusarium solani (fusarium solani) by applying a bactericidal composition comprising tetrazolium picolinate and hymexazol to plant propagation material and subsequently growing plant organs.
A method for reducing or preventing crop infestation by Fusarium oxysporum tomato neck rot root rot transformant bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthosporium (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum (Fusarium graminearum), fusarium candidum (Fusarium moniliforme), fusarium moniliforme (Fusarium moniliforme), fusarium solani (Fusarium solani) pathogenic bacteria forming toxins, by applying a bactericidal composition containing tetrazolium picolinate and hymexazol to soil or cultivation medium.
The time of application, the number of applications and the rate of application used in each case must be adapted to the prevailing conditions and must be determined by the person skilled in the art for each individual case.
Treatment with a bactericidal composition containing tetrazolium picolinate and hymexazol may be protective and therapeutic. Either before or after infestation by the harmful fungi. Preferably at a time as close as possible to the infestation, i.e. before or after the infestation at a point in time as close as possible to the infestation.
The bactericidal composition containing the tetrazole picolide and the hymexazol can be used as a foliar fungicide in crop protection, can be used as a fungicide for seed dressing and soil fungicide, and can be used as a preservative for post-harvest storage.
The bactericidal composition containing the tetrazole picolide and the hymexazol can treat all crops. "crop" refers to all plants and plant populations such as desirable and undesirable wild plants, cultivated plants and plant varieties (whether protected by plant varieties or plant growing rights). The cultivated plants and plant varieties may be plants obtained by conventional propagation and cultivation methods, which may be supplemented or supplemented with one or more biotechnological methods, for example using doubled haploids, protoplast fusion, random and directed mutations, molecular or genetic markers, or using bioengineering and genetic engineering methods. Plant parts refer to all above and below ground parts and organs of plants, such as shoots, leaves, flowers and roots, such as leaves, needles, stems, branches, flowers, fruit bodies, fruits and seeds as well as roots, bulbs and rhizomes. Crops as well as vegetative propagation and propagation material, for example cuttings, bulbs, rhizomes, fibrins and seeds, also belong to the plant part.
Suitable crops include mainly field crops, such as corn, soybean, cotton, canola seeds, such as nappa (Brassica napus), mustard (e.g. mustard), rice, wheat, beet, sugarcane, oat, brown barley, millet, rye, flax, grape vine and fruit or vegetable crops of various plant categories, such as rosaceous fruits, such as apples and pears, as well as stone fruits, such as apricots, cherries, almonds and peaches, such as strawberries, tea-sugarcane sp, walnut sp, birch sp, lacquer sp, fagaceae, moraceae, oleaceae, actiaceae, lauraceae, juhance, and Jutaceae, such as Musaceae, muslimidaceae, and Juhance, such as guaiaceae, juglandaceae, and Jutaceae; solanaceae (solanaceae sp.) (e.g., tomato, potato, pepper, eggplant), liliaceae (Liliaceae sp.) (e.g., lettuce, artichoke, and chicory-including root chicory (root chiry), endive (endive), or common chicory (common chicory)), umbelliferae sp.) (e.g., carrot, parsley, celery, and root celery), cucurbitaceae (cuurbitaceae sp.) (e.g., cucumber-including pickled cucumber (pickling cucumber), pumpkin, watermelon, cucurbit, and melon), alliaceae (Alliaceae sp.) (e.g., onion and leek), cruciferaceae (cricket) stem (e.g., white cabbage, red cabbage, cauliflower, broccoli, cabbage, head, cabbage, radish, horseradish, row, soybean), legume (e.g., carrot, parsley, and root celery) (e.g., spinach), and spinach (e.g., sweet beet) (e.g., sweet potato, spinach); horticultural crops and forest crops; ornamental plants; and genetically modified homologs of these crops. Solanaceae (solanaceae.) (e.g. tomato, potato, pepper, eggplant) are preferred.
The term "plant propagation material" is understood to mean all plant parts, such as seeds, which have reproductive capacity and which can be used for propagating the latter, as well as plant materials such as cuttings or tubers. Thus, plant parts as used herein include plant propagation material. Mention may be made, for example, of seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated plants to be germinated from soil or suppressed after emergence of seedlings, and effective plants. Young plants may be protected by impregnation, either entirely or partially, prior to implantation.
The preferred plant propagation material of the invention is a seed. The bactericidal composition containing the tetrazole picolide and the hymexazol is also particularly suitable for treating seeds. Most crop damage caused by harmful fungi is due to attack of seeds during storage or after sowing and during or after germination of the plants. Plants die because roots and shoots of growing plants are particularly sensitive and even small lesions can result. This stage is particularly critical, since it is important to protect the seeds and germinated plants by using suitable compositions.
The invention also relates in particular to the reduction or prevention of infestation of seeds and germinated plants by toxins formed by Fusarium oxysporum tomato neck rot root rot transformant (Fusarium oxysporum f.sp.radicis-lycopersici), fusarium solani (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium candidum (Fusarium moniliforme), fusarium moniliforme (Fusarium pro-interference), fusarium solani (Fusarium solani) pathogens by treating the seeds with the bactericidal composition containing tetrazolium and hymexazol according to the present invention. The method eliminates the need for additional or at least significant additional application of the crop protection agent after sowing or after germination of the plant. On the other hand, the amount of active compound used is optimized with the fungicidal composition according to the invention to provide maximum protection of seeds and germinating plants from phytopathogenic fungi, without the plants themselves being damaged by the active compound used.
The bactericidal composition containing the tetrazolium picolinate and the hymexazol is suitable to be treated in a sufficiently stable state so that the treatment does not cause any damage to seeds. In general, the treatment of seeds can be performed at any point in time between picking and sowing. The seed used is usually isolated from the plant and from the cob, husk, stem, epidermis, hair or pulp. Thus, for example, seeds that have been picked, cleaned and dried to a moisture content of less than 15% may be used. Alternatively, seeds which have been dried, for example by treatment with water, and then dried again can also be used.
Examples of the method for treating the seed include a method of immersing the seed in a liquid state solution by diluting a liquid or solid chemical or directly immersing the seed in a liquid state solution without dilution, a method of mixing the solid chemical or liquid chemical with the seed, a method of coating the seed with a chemical, and a method of spraying the seed with a chemical while planting.
The bactericidal composition containing tetrazolium picolinate and hymexazol is particularly beneficial to seed treatment, especially for seeds of tomatoes, peppers, potatoes, tobacco, oilseed rape, cotton, soybeans, corns, peanuts, alfalfa, medlar, cucumbers, barley, wheat and rice. Seeds of tomato, capsicum, potato, tobacco, matrimony vine, alfalfa are preferred.
The control of phytopathogenic fungi damaging post-emergence plants is mainly carried out by treating the soil and the aerial parts of the plants with a bactericidal composition containing tetrazolium picolinate and hymexazol according to the invention.
The bactericidal composition containing tetrazolium picolinate and hymexazol can also be used for treating soil to reduce or prevent the infection of crops by toxins formed by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp. radicis-lycopersici), fusarium solani (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium moniliforme (fusarium moniliforme), fusarium solani (fusarium solani) pathogenic bacteria.
Methods of applying the agent to the soil include methods of spraying the "fungicidal composition" onto the soil, methods of mixing the "fungicidal composition" with the soil, and methods of irrigating the "fungicidal composition" into the soil.
The bactericidal composition containing the tetrazolium picolinate and the hymexazol can be used for treating plants or aerial parts of plants to reduce or prevent infection of crops by toxins formed by fusarium oxysporum neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), fusarium solani (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium candidum (fusarium moniforme), fusarium moniliforme (fusarium pro-ferratum) and fusarium solani (fusarium solani). When applied to plants or plant parts, the composition is applied in the form of an aqueous spray. The application is preferably carried out by spraying. The spray liquid is sprayed on the whole ground part of the plant or only on each plant part. The choice of the individual plant parts to which the spray liquid is to be applied depends on the plant species and the stage of development.
The bactericidal composition containing tetrazolium picolinate and hymexazol can also be used for treating stock to reduce or prevent infection of toxins formed by fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp. radicis-lycopersici), tomato acanthocellum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium moniliforme (fusarium moniliforme), fusarium solani (fusarium solani) pathogenic bacteria.
According to the invention, the term "stock" is understood to mean natural substances of vegetable or animal origin and processed forms thereof which have been derived from the natural life cycle and which are intended to be preserved for a long period of time. Storage of plant origin, for example plants or parts thereof, such as stems, leaves, tubers, seeds, fruits or grains, may be protected in freshly harvested state or in processed form, such as (pre) drying, wetting, crushing, grinding, pressing or baking. But also wood, in the form of raw wood such as construction lumber, utility poles and fences; or in finished form, such as furniture or articles made of wood. The animal-derived stock is hide, leather, hair, etc. The composition according to the invention prevents fungal or bacterial attack such as corrosion, discoloration or mildew during storage. "stock" is preferably understood to mean natural substances of vegetable origin and processed forms thereof.
The bactericidal composition containing the tetrazole picolide and the hymexazol can be treated by different treatment methods:
-spraying a liquid comprising the bactericidal composition onto the aerial parts of the plants;
-dusting, incorporating granules or powder in the soil, spraying around the plants, and in the case of tree injection or painting;
-coating or film-coating the seeds of the plants.
The invention provides a method for reducing or preventing crop infection by toxins formed by fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici), tomato acanthocellum (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium moniliforme (fusarium pro-interference), fusarium solani (fusarium solani) pathogenic bacteria, which can be a treatment, prevention or eradication method.
For leaf processing in general: 0.1-10000g/ha, preferably 10-1000 g/ha, more preferably 50-500 g/ha; for dip or drip application, the dosage may even be reduced, especially when an inert substrate such as asbestos or perlite is applied;
-for seed treatment: 2-5000g/100kg seed, preferably 3-1000g/100kg seed;
-applying a treatment to the soil or water surface: 0.1-10000g/ha, preferably 1-1000g/ha.
The above dosages are merely exemplary dosages in general, and the person skilled in the art will adjust the application rate according to the actual situation and the needs, in particular according to the nature of the plant or crop to be treated and the seriousness of the pathogen at the time of actual application.
Indoor toxicity measurement
Test one: determination of virulence of Fusarium oxysporum tomato neck rot root rot transformed bacterium (Fusarium oxysporum f.sp.radicis-lycopersici)
The method for inhibiting the hypha growth rate is adopted:
dissolving tetrazolium picolinate and hymexazol with acetone respectively, diluting with 0.1% Tween-80 aqueous solution to obtain serial concentration liquid medicine, respectively sucking 6mL into sterilized Erlenmeyer flasks in an ultra-clean workbench, adding 54mL of potato dextrose agar medium (PDA) at about 50deg.C, shaking, and pouring into 4 plates with diameter of 9cm to obtain 4 toxic culture mediums with corresponding concentrations; the same method is used for preparing the toxic culture medium by compounding the tetrazole picoline and hymexazol series concentration compound liquid with different proportions. Fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici) were cultured for 2 days, the bacterial blocks were punched at the edges of the colonies by a puncher with a diameter of 5mm, the bacterial blocks were moved to the center of a previously prepared toxic PDA medium by an inoculating needle, and then placed in a 25 ℃ incubator for culture, and each treatment was repeated 4 times. After 3 days, the diameter cm of each treated colony was measured by a caliper using a crisscross method, and the percent inhibition was corrected. Each colony was cross-sectioned for two diameters, with the average representing colony size. Then, the colony growth inhibition ratio was determined as follows:
Then the concentration EC in inhibition is calculated by a least square method 50 And calculating a co-toxicity coefficient (CTC) by using a grandchild cloud method.
Table 1: toxicity test results on Fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici)
As is clear from Table 1, when the ratio of the combination of tetrazolium picolinate and hymexazol is in the range of 50:1-1:5, the co-toxicity coefficients of the combination of tetrazolium picolinate and hymexazol to Fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici) are all larger than 120, and the combination shows a synergistic effect.
And (2) testing II: toxicity assay for tomato echinococcosis (Pyrenochaeta lycopersici Schneider et Ger lach)
The method for inhibiting the hypha growth rate is adopted:
dissolving tetrazolium picolinate and hymexazol with acetone respectively, diluting with 0.1% Tween-80 aqueous solution to obtain serial concentration liquid medicine, respectively sucking 6mL into sterilized Erlenmeyer flasks in an ultra-clean workbench, adding 54mL of potato dextrose agar medium (PDA) at about 50deg.C, shaking, and pouring into 4 plates with diameter of 9cm to obtain 4 toxic culture mediums with corresponding concentrations; the same method is used for preparing the toxic culture medium by compounding the tetrazole picoline and hymexazol series concentration compound liquid with different proportions. 2 days of culture of tomato acanthocephalus (Pyrenochaeta lycopersici Schneider et Ger lach), the bacterial block was punched at the edge of the colony with a punch with a diameter of 5mm, the bacterial block was transferred to the center of the previously prepared toxic PDA medium with an inoculating needle, and then placed in a 25℃incubator for culture, and each treatment was repeated 4 times. After 3 days, the diameter cm of each treated colony was measured by a caliper using a crisscross method, and the percent inhibition was corrected. Each colony was cross-sectioned for two diameters, with the average representing colony size. Then, the colony growth inhibition ratio was determined as follows:
Then the concentration EC in inhibition is calculated by a least square method 50 And calculating a co-toxicity coefficient (CTC) by using a grandchild cloud method.
Table 2: toxicity test results on tomato acanthocephalum (Pyrenochaeta lycopersici Schneider et Ger lach)
As can be seen from Table 2, when the combination of tetrazolium picolinate and hymexazol is in the range of 50:1-1:50, the co-toxicity coefficient of the combination on tomato acanthamoxicillin (Pyrenochaeta lycopersici Schneider et Ger lach) is more than 120, and the combination shows a synergistic effect.
And (3) test III: toxicity assay of Fusarium graminearum
The method for inhibiting the hypha growth rate is adopted:
dissolving tetrazolium picolinate and hymexazol with acetone respectively, diluting with 0.1% Tween-80 aqueous solution to obtain serial concentration liquid medicine, respectively sucking 6mL into sterilized Erlenmeyer flasks in an ultra-clean workbench, adding 54mL of potato dextrose agar medium (PDA) at about 50deg.C, shaking, and pouring into 4 plates with diameter of 9cm to obtain 4 toxic culture mediums with corresponding concentrations; the same method is used for preparing the toxic culture medium by compounding the tetrazole picoline and hymexazol series concentration compound liquid with different proportions. Fusarium graminearum (Fusarium graminearum) was cultured for 2 days, the colonies were pelleted at the edges thereof by a punch with a diameter of 5mm, the colonies were transferred to the center of a previously prepared toxic PDA medium by a inoculating needle, and then placed in a 25℃incubator for culture, and each treatment was repeated 4 times. After 3 days, the diameter cm of each treated colony was measured by a caliper using a crisscross method, and the percent inhibition was corrected. Each colony was cross-sectioned for two diameters, with the average representing colony size. Then, the colony growth inhibition ratio was determined as follows:
Then the concentration EC in inhibition is calculated by a least square method 50 And calculating a co-toxicity coefficient (CTC) by using a grandchild cloud method.
Table 3: toxicity test results on Fusarium graminearum (Fusarium graminearum)
As can be seen from Table 3, when the combination of tetrazolium picolinate and hymexazol is in the range of 1:15-1:50, the co-toxicity coefficient of Fusarium graminearum (Fusarium graminearum) is greater than 120, and the synergistic effect is shown.
And (3) testing four: toxicity assay for Fusarium moniliforme
The method for inhibiting the hypha growth rate is adopted:
dissolving tetrazolium picolinate and hymexazol with acetone respectively, diluting with 0.1% Tween-80 aqueous solution to obtain serial concentration liquid medicine, respectively sucking 6mL into sterilized Erlenmeyer flasks in an ultra-clean workbench, adding 54mL of potato dextrose agar medium (PDA) at about 50deg.C, shaking, and pouring into 4 plates with diameter of 9cm to obtain 4 toxic culture mediums with corresponding concentrations; the same method is used for preparing the toxic culture medium by compounding the tetrazole picoline and hymexazol series concentration compound liquid with different proportions. Fusarium moniliforme (Fusarium moniliforme) cultured for 2 days was inoculated with a punch with a diameter of 5mm to form a pellet at the edge of the colony, the pellet was transferred to the center of a previously prepared toxic PDA medium with an inoculating needle, and then cultured in a 25℃incubator, and each treatment was repeated 4 times. After 3 days, the diameter cm of each treated colony was measured by a caliper using a crisscross method, and the percent inhibition was corrected. Each colony was cross-sectioned for two diameters, with the average representing colony size. Then, the colony growth inhibition ratio was determined as follows:
Then the concentration EC in inhibition is calculated by a least square method 50 And calculating a co-toxicity coefficient (CTC) by using a grandchild cloud method.
Table 4: toxicity test results on Fusarium moniliforme (Fusarium moniliforme)
As can be seen from Table 4, when the combination of tetrazolium picolinate and hymexazol is in the range of 1:15-1:50, the co-toxicity coefficient of fusarium moniliforme (Fusarium moniliforme) is greater than 120, and the synergistic effect is shown.
Test five: virulence assay of Fusarium moniliforme (Fusarium proliferatum)
The method for inhibiting the hypha growth rate is adopted:
dissolving tetrazolium picolinate and hymexazol with acetone respectively, diluting with 0.1% Tween-80 aqueous solution to obtain serial concentration liquid medicine, respectively sucking 6mL into sterilized Erlenmeyer flasks in an ultra-clean workbench, adding 54mL of potato dextrose agar medium (PDA) at about 50deg.C, shaking, and pouring into 4 plates with diameter of 9cm to obtain 4 toxic culture mediums with corresponding concentrations; the same method is used for preparing the toxic culture medium by compounding the tetrazole picoline and hymexazol series concentration compound liquid with different proportions. Fusarium moniliforme (Fusarium proliferatum) was cultured for 2 days, the bacterial block was punched at the edge of the colony with a punch with a diameter of 5mm, the bacterial block was transferred to the center of a previously prepared toxic PDA medium with an inoculating needle, and then placed in a 25℃incubator for culture, and each treatment was repeated 4 times. After 3 days, the diameter cm of each treated colony was measured by a caliper using a crisscross method, and the percent inhibition was corrected. Each colony was cross-sectioned for two diameters, with the average representing colony size. Then, the colony growth inhibition ratio was determined as follows:
Then the concentration EC in inhibition is calculated by a least square method 50 And calculating a co-toxicity coefficient (CTC) by using a grandchild cloud method.
Table 5: results of toxicity test on Fusarium moniliforme (Fusarium proliferatum)
As can be seen from table 5, when the combination of tetrazolium picolinate and hymexazol is in the range of 1:15-1:50, the co-toxicity coefficient for fusarium moniliforme (fusarium proliferatum) is greater than 120, and the synergistic effect is exhibited.
Test six: virulence assay of Fusarium solani (Fusarium solani)
The method for inhibiting the hypha growth rate is adopted:
dissolving tetrazolium picolinate and hymexazol with acetone respectively, diluting with 0.1% Tween-80 aqueous solution to obtain serial concentration liquid medicine, respectively sucking 6mL into sterilized Erlenmeyer flasks in an ultra-clean workbench, adding 54mL of potato dextrose agar medium (PDA) at about 50deg.C, shaking, and pouring into 4 plates with diameter of 9cm to obtain 4 toxic culture mediums with corresponding concentrations; the same method is used for preparing the toxic culture medium by compounding the tetrazole picoline and hymexazol series concentration compound liquid with different proportions. Fusarium solani (Fusarium solani) cultured for 2 days was inoculated with a punch with a diameter of 5mm at the edge of the colony to form a pellet, the pellet was transferred to the center of a previously prepared toxic PDA medium with an inoculating needle, and then cultured in a 25℃incubator, and each treatment was repeated 4 times. After 3 days, the diameter cm of each treated colony was measured by a caliper using a crisscross method, and the percent inhibition was corrected. Each colony was cross-sectioned for two diameters, with the average representing colony size. Then, the colony growth inhibition ratio was determined as follows:
Then the concentration EC in inhibition is calculated by a least square method 50 And calculating a co-toxicity coefficient (CTC) by using a grandchild cloud method.
Table 6: results of toxicity test on Fusarium solani (Fusarium solani)
As can be seen from Table 6, when the combination of tetrazolium picolinate and hymexazol is in the range of 1:15-1:50, the co-toxicity coefficient of Fusarium solani (Fusarium solani) is greater than 120, and the synergistic effect is exhibited.
Test of efficacy
Test 1 Activity/seed treatment on Fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici)
The plastic pots were filled with soil infested with Fusarium oxysporum tomato neck rot root rot transformant (Fusarium oxysporum f.sp. radicis-lycopersici), tomato acanthosporium (Pyrenochaeta lycopersici Schneider et Ger lach), fusarium graminearum, fusarium candidum (Fusarium moniliforme), fusarium moniliforme (Fusarium pro-ferum), fusarium solani (Fusarium solani), and tomato seeds treated with the test compound were inoculated into the soil and cultivated in a greenhouse for 20 days.
The infestation by pathogenic fungi from each germinated young plant was observed, and the extent of disease infestation in the "treatment samples" was calculated according to the following calculation formula (1).
On the other hand, tomato seeds not treated as above were cultivated in the same manner as above, the infestation by pathogenic fungi from each germinated young plant was observed, and the extent of disease infestation in the "untreated sample" was calculated according to the following calculation formula (1).
Based on the degree of disease infestation in the "treated sample" and the degree of disease infestation in the "untreated sample", the efficacy in the "treated sample" is calculated according to the following calculation formula (2).
Calculation formula (1):
degree of pest infestation (%) = (number of infected young plants/total young plants) ×100
Calculation formula (2):
efficacy (%) = [1- ("treatment-like" disease infection degree/"untreated-like" disease infection degree) ]. 100
TABLE 1 efficacy against Fusarium oxysporum tomato neck rot root rot transformant (Fusarium oxysporum f.sp.radicis-lycopersici)
The test results in Table 1 show that the combination of tetrazolium picolinate and hymexazol has excellent control effects on Fusarium oxysporum tomato neck rot root rot transformed bacteria (Fusarium oxysporum f.sp.radicis-lycopersici).
TABLE 2 efficacy against Acremonium lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach)
Table 2 the test results show that the combination of tetrazolium picolinate and hymexazol has excellent control of aschersonia lycopersicum (Pyrenochaeta lycopersici Schneider et Ger lach).
TABLE 3 efficacy against Fusarium graminearum
Table 3 shows that the combination of tetrazolium picolinate and hymexazol has excellent control of Fusarium graminearum.
TABLE 4 efficacy against Fusarium moniliforme (Fusarium moniliforme)
Table 4 the test results show that the combination of tetrazolium picolinate and hymexazol has excellent control over fusarium moniliforme.
TABLE 5 efficacy against Fusarium moniliforme (Fusarium proliferatum)
Table 5 the test results show that the combination of tetrazolium picolinate and hymexazol has excellent control of fusarium moniliforme (fusarium proliferatum).
TABLE 6 efficacy against Fusarium solani (Fusarium solani)
Table 6 shows that the combination of tetrazolium picolinate and hymexazol has excellent control of Fusarium solani (Fusarium solani).
Claims (13)
1. Use of a bactericidal composition containing tetrazolium picolinate and hymexazol for reducing or preventing crop infection by Fusarium graminearum (Fusarium graminearum), fusarium candidum (Fusarium moniliforme), fusarium moniliforme (Fusarium proliferatum) and Fusarium solani (Fusarium solani) pathogenic bacteria, wherein the bactericidal composition containing tetrazolium picolinate and hymexazol comprises the weight ratio of tetrazolium picolinate to hymexazol is 1:15-1:50.
2. The use according to claim 1, wherein the weight ratio of the tetrazole picoline to the hymexazol in the bactericidal composition containing the tetrazole picoline and the hymexazol is 1:15-1:40.
3. The use according to claim 1, wherein the weight ratio of the tetrazole picoline to the hymexazol in the bactericidal composition containing the tetrazole picoline and the hymexazol is 1:15-1:35.
4. The use according to claim 1, wherein the weight ratio of the tetrazole picoline to the hymexazol in the bactericidal composition containing the tetrazole picoline and the hymexazol is 1:15-1:30.
5. The use according to claim 1, wherein the weight ratio of the tetrazole picoline to the hymexazol in the bactericidal composition containing the tetrazole picoline and the hymexazol is 1:15-1:25.
6. The use according to claim 1, wherein the crop is selected from potato, pepper, eggplant, alfalfa, tomato, capsicum, tobacco, canola, cotton, soybean, maize, peanut, medlar, cucumber, barley, wheat, rice.
7. The use according to claim 1, characterized in that in the bactericidal composition containing tetrazole picolide and hymexazol, the total weight of the bactericidal composition is 1-90%.
8. The use according to claim 7, wherein the total weight of the bactericidal composition containing tetrazole picolide and hymexazol is 1-60%.
9. The use according to claim 7, wherein the total weight of the bactericidal composition containing tetrazole picolide and hymexazol is 5-50%.
10. The use according to claim 1, wherein the bactericidal composition containing tetrazole picolide and hymexazol is in the form of a suspension, a seed-treated dry powder, a seed-treated dispersible powder, a seed-treated microcapsule suspension, a seed-treated suspension, a suspoemulsion, a wettable powder, a water dispersible granule, a microcapsule suspension, a coated granule, an extruded granule, an emulsifiable concentrate, a microemulsion, an aqueous emulsion, an effervescent tablet, an ultra-low volume liquid.
11. A method for reducing or preventing crop infestation by Fusarium graminearum (Fusarium graminearum), fusarium candidum (Fusarium moniliforme), fusarium moniliforme (Fusarium proliferatum), fusarium solani (Fusarium solani) pathogens, characterized in that the pathogens and/or their environment, or plants, plant propagation material and subsequently growing plant organs, soil, material or space, stock are treated with a fungicidal composition according to claim 1.
12. A method according to claim 11, characterized in that the pathogenic bacteria and/or their environment, or the plants, plant propagation material and subsequently growing plant organs, soil, materials or spaces, storages are treated with a bactericidal composition containing tetrazolium picolide and hymexazol in seed treatment, foliar application, stem application, saturation, instillation, pouring, spraying, atomizing, dusting, scattering or fuming manner.
13. The method of claim 11, wherein the treatment is performed before or after infestation by pathogenic bacteria.
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| 李丽娜 等.四种杀菌剂对玉米穗腐病菌的室内毒力测定.《绿色生态可持续发展与植物保护——中国植物保护学会第十二次全国会员代表大会暨学术年会论文集》.2017,272. * |
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