CN110810035A - Asexual propagation method of phyllanthus emblica - Google Patents
Asexual propagation method of phyllanthus emblica Download PDFInfo
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- CN110810035A CN110810035A CN201911180026.8A CN201911180026A CN110810035A CN 110810035 A CN110810035 A CN 110810035A CN 201911180026 A CN201911180026 A CN 201911180026A CN 110810035 A CN110810035 A CN 110810035A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Abstract
The invention discloses a vegetative propagation method of phyllanthus emblica.A branch of the current-season aged phyllanthus emblica is cut and inserted on a seedling bed, the tender shoots are generated until the second-time shoots are aged, the phyllanthus emblica is transplanted into a seedling bag filled with a breeding substrate, then the PCR detection is carried out on the leaves of the breeding seedlings, and if the germ detection of a virulent infectious disease is negative, the phyllanthus emblica is healthy and can be planted out of a nursery. The method for cutting the citrus reticulata blanco can improve the survival rate of cutting, the cut branches are easy to root, the success rate is high, the occurrence of yellow dragon disease in the seedling stage is avoided, and the method is suitable for industrial production.
Description
Technical Field
The invention belongs to the field of fruit tree planting, and particularly relates to a vegetative propagation method of phyllanthus emblica.
Background
The pericarpium Citri Tangerinae is dry pericarp of Rutaceae plant fructus Citri Tangerinae and its cultivar, is divided into "Guang pericarpium Citri Tangerinae" and "pericarpium Citri Tangerinae", and has effects of regulating qi-flowing, invigorating spleen, regulating stomach, relieving vomit, eliminating dampness and eliminating phlegm, etc. The pericarpium citri reticulatae is the top grade of pericarpium citri reticulatae and has important medicinal value. The citrus reticulata blanco is a raw material for preparing the citrus reticulata blanco, the Xinhui city in Guangdong province is a main production area for preparing the citrus reticulata blanco, the planting scale of the citrus reticulata blanco in the market reaches more than twenty thousand mu at present, and the planting of the citrus reticulata blanco becomes a local leading industry.
The citrus yellow shoot is one of the main diseases of citrus, and after infection, the leaves of plants gradually turn yellow, fruits are stiff, and the whole plant is dry and dead in severe cases. The citrus yellow shoot is a bacterial disease, no effective control agent exists at present, the bacterial disease is mainly transmitted through germ-carrying seedlings and field germ-carrying citrus psyllids, the incubation period of the germ can be as long as more than 3 years, the germ is difficult to judge by naked eyes in the early stage, and the citrus yellow shoot is the primary disease of the citrus industry. The phyllanthus emblica is susceptible to citrus greening disease like other citrus plants, but the limitation standard of pesticide residues is more strict because the phyllanthus emblica generates pericarp with medicinal value and economic value, and the use requirement of pesticides in the cultivation process is more strict. Generally, after the second physiological fruit drop of the phyllanthus emblica fruit, the insecticide is basically not used until the fruit harvest is finished. This provides time and space for the propagation of diaphorina citri, for example, if the nursery stock planted in the orchard is partially infected with bacteria or has been infected with the yellow shoot, this creates a good opportunity for the spread of the yellow shoot of citrus. Therefore, the quality of the citrus reticulata blanco seedlings is strictly controlled, the fact that the planted seedlings do not carry citrus yellow dragon germs is guaranteed, and the method is the primary factor for blocking the yellow dragon germs from spreading in citrus reticulata blanco orchards.
At present, the tea branch citrus seedlings are mainly divided into two types, namely grafted seedlings and circled seedlings. The grafted seedling is obtained by grafting the bud of the phyllanthus emblica on a proper stock for breeding, the seedling has the advantages of vigorous growth, more fruits and the like, but most people think that the stock of the grafted seedling can change the original characteristics of the phyllanthus emblica and have certain influence on the drug property and the like, so the market acceptance is not high and the price is low. The circled branch seedling is a citrus reticulata seedling which is widely advocated by local people, and is a seedling which is planted after being cut and rooted by coating mixed liquid such as adhesive cement on an aged branch on a citrus reticulata plant and then wrapping the aged branch with plastic paper, rice straw and the like. However, the breeding of the circled branch seedlings needs to be carried out on the phyllanthus emblica plants in the field, the yellow dragon disease is difficult to ensure to be avoided, and even if the parent plants do not carry bacteria, the breeding branches are exposed for a long time in the breeding process, and the risk of infection by the bacteria-carrying diaphorina citri exists. In addition, the seedling of the circled branch also has the defects of slow rooting, long seedling period, inconvenience for centralized management and the like.
The citrus seedling raising mostly adopts a grafting technology, and the asexual propagation is rarely carried out by applying a cuttage technology at present. In view of the above problems, the present invention has been made in an effort to develop a novel method for vegetative propagation of Citrus reticulata.
Disclosure of Invention
The invention aims to provide a method for vegetative propagation of phyllanthus emblica.
The technical scheme adopted by the invention is as follows:
a vegetative propagation method of phyllanthus emblica comprises the following steps:
1) cutting off the peripheral season-old branches after the mother plant of the phyllanthus emblica is placed at the tip, cutting the branches, and reserving two leaves at the middle upper part;
2) bundling the citrus reticulata branch into a bundle, soaking the bundle in a prepared rooting powder solution, taking out the bundle, then cutting the bundle to a seedbed, inserting bamboo chips into one side of the seedbed, bending the bamboo chips into an arch, then inserting the bamboo chips into soil of the seedbed on the other side, fixing the bamboo chips, and covering the arched bamboo chips with a plastic film;
3) after cuttage, uniformly and thoroughly spraying the seedbed and spraying a bactericide;
4) after tender shoots of branches of the tea oranges are extracted, spraying an amino acid foliar fertilizer every other week until the first shoots are aged, then spraying a foliar fertilizer every other week, and broadcasting a high-nitrogen compound fertilizer between two rows of seedlings and spraying water until the second shoots are aged; preferably, in hot and humid days, the two ends of the plastic film are rolled up for ventilation;
5) watering the second-time mature cutting seedling, then transplanting the seedling into a seedling bag filled with breeding matrix, watering and shading.
Further, the method comprises the steps of sampling and breeding leaves of the seedlings, grinding, extracting DNA, detecting whether the seedlings carry pathogenic bacteria of the virulent infectious diseases or not, detecting the seedlings as positive as the citrus reticulata blanco carrying the pathogenic bacteria, and detecting the seedlings as negative as the healthy citrus reticulata blanco seedlings to carry out outplanting planting.
Furthermore, the virulent infectious disease is citrus yellow dragon disease.
Further, the aged branches in the step 1) are aged branches 15-20 days after tip releasing in season; preferably, when the branches in the season are mature, the branches are cut into branches of 10-15 cm.
Further, the seedbed in the step 2) is a strip-shaped ridge, and soil with the thickness of 30-50 cm and the width of 1-1.5 m is paved on the ridge.
Further, bundling 50-100 citrus reticulata branches into a bundle.
Further, the citrus reticulata blanco in the step 2) is cut to the depth of 3-6 cm of the seedbed, the row spacing of the citrus reticulata blanco cutting is 8-10 cm, and the plant spacing is 8-10 cm; preferably, the bamboo chips are inserted into the seedbed from one side to 8-10 cm deep, bent into an arch shape and then inserted into the seedbed soil on the other side for fixing.
Further, the tea branch and the citrus reticulata branch can be inserted into the seedling raising cup in the step 2).
Further, the bactericide in the step 3) is a broad-spectrum bactericide; the broad-spectrum bactericide is mancozeb, chlorothalonil and/or a copper preparation.
Further, the breeding substrate in the step 5) comprises 10 parts of coconut husk peat soil mixture and 1 part of compound fertilizer or other mixture ratios according to the performance of the fertilizer.
The inventor uses a grafting method and a lopping method to plant the phyllanthus emblica. However, the grafted tea branch citrus seedlings have the phenomenon that the citrus fruit genes are not pure due to the rootstocks; the problem that the seedling of the circus has slow rooting and high probability of infecting citrus yellow shoot at the seedling stage. Therefore, the invention provides a novel method for vegetative propagation of branches of the phyllanthus emblica, which can effectively prevent the phyllanthus emblica seedlings from infecting citrus yellow dragon disease.
The invention has the beneficial effects that:
aiming at the phenomena that the grafted seedling of the citrus reticulata has the influence of a stock to cause an impure phenomenon, and the seedlings with the ring branches have slow rooting and can not be managed in a centralized way, and the field has the risk of infecting yellow dragon disease; the asexual propagation technology of the phyllanthus emblica does not need stock grafting, can concentrate greenhouse isolation and propagation, obviously shortens the rooting time, ensures that the propagated nursery stocks are healthy and pure in gene, effectively avoids the risk of citrus yellow shoot at the seedling stage, obviously improves the production efficiency of the healthy phyllanthus emblica nursery stocks and ensures the sustainable development of the phyllanthus emblica industry.
Healthy mother plant trees are cultivated in a net room, cuttage branches are prevented from infecting citrus yellow shoot disease, and the incidence rate is lower than that of comparative example circle seedlings. In addition, the seedlings are planted in the arched shed or the cup in the net room in a cutting mode, the fact that the seedlings are infected by citrus yellow shoot is avoided in the period, and virus-free production in the whole seedling period is achieved.
Drawings
FIG. 1 is a schematic view of a branch of Citrus reticulata before planting.
Fig. 2 is a schematic diagram of the citrus reticulata after being planted by the cuttage method of the embodiment 1.
Fig. 3 is a schematic diagram of the plastic film added after the citrus reticulata is planted by the cuttage method of the embodiment 1.
Fig. 4 shows the phyllanthus emblica after being planted by the cuttage method of the embodiment 1 of the invention.
Detailed Description
A vegetative propagation method of phyllanthus emblica comprises the following steps:
1) cutting off the peripheral mature branches in the season after the mother plant of the phyllanthus emblica is placed at the tip, cutting the branches, and reserving two leaves at the middle upper part;
2) bundling the citrus reticulata branch into a bundle, soaking the bundle in a prepared rooting powder solution, taking out the bundle, then cutting the bundle to a seedbed, inserting bamboo chips into one side of the seedbed, bending the bamboo chips into an arch, then inserting the bamboo chips into soil of the seedbed on the other side, fixing the bamboo chips, and covering the arched bamboo chips with a plastic film;
3) after cuttage, uniformly and thoroughly spraying the seedbed and spraying a bactericide;
4) after tender shoots of branches of the tea oranges are extracted, spraying an amino acid foliar fertilizer every other week until the first shoots are aged, then spraying a foliar fertilizer every other week, and broadcasting a high-nitrogen compound fertilizer between two rows of seedlings and spraying water until the second shoots are aged; preferably, in hot and humid days, the two ends of the plastic film are rolled up for ventilation;
5) watering the second-time mature cutting seedling, then transplanting the seedling into a seedling bag filled with breeding matrix, watering and shading.
Preferably, the method further comprises the steps of sampling and breeding leaves of the seedlings, grinding, extracting DNA, detecting whether the seedlings carry pathogenic bacteria of the virulent infectious diseases or not, detecting the seedlings as positive citrus reticulata carrying pathogenic bacteria, and detecting the seedlings as negative citrus reticulata carrying pathogenic bacteria to carry out outplanting planting on the healthy citrus reticulata seedlings.
Preferably, the virulent infectious disease is citrus yellow dragon disease.
Preferably, the aged branches in the step 1) are aged branches 15-20 days after tip releasing in season; preferably, when the branches in the season are mature, the branches are cut into branches of 10-15 cm.
Preferably, the seedbed in the step 2) is a strip-shaped ridge, and soil with the thickness of 30-50 cm and the width of 1-1.5 m is paved on the ridge.
Preferably, 50-100 citrus reticulata branches are bundled in the step 2).
Preferably, the citrus reticulata blanco in the step 2) is cut to the depth of 3-6 cm of the seedbed, the row spacing of the citrus reticulata blanco cutting is 8-10 cm, and the plant spacing is 8-10 cm; preferably, the bamboo chips are inserted into the seedbed from one side to 8-10 cm deep, bent into an arch shape and then inserted into the seedbed soil on the other side for fixing.
Preferably, the bactericide in the step 3) is a broad-spectrum bactericide; the broad-spectrum bactericide is mancozeb, chlorothalonil and/or a copper preparation.
The broad-spectrum bactericide in the step 3) is but not limited to mancozeb, chlorothalonil and/or a copper preparation.
Preferably, the breeding substrate in the step 5) comprises 10 parts of coconut husk peat soil mixture and 1 part of compound fertilizer or other mixture ratio according to the performance of the fertilizer.
Preferably, the citrus reticulata branch in the step 2) can be inserted into a seedling raising cup.
Early preparation
1. Mother plant breeding garden for phyllanthus emblica
And extracting DNA of the plant leaves in the breeding nursery, and detecting whether the plant leaves are positive by PCR (polymerase chain reaction), wherein the positive is carried with germs, and the negative is not carried.
The method comprises the following specific steps: firstly, sampling leaves of nursery stocks in nursery gardens, shearing leaf veins, grinding in liquid nitrogen, adding a CTAB buffer solution with the same volume, carrying out water bath at 65 ℃ for 30min, adding chloroform/isoamylol with the same volume, centrifuging at 12000r/min for 15min, taking supernate, adding isopropanol with the volume of 0.6-1 time, standing at room temperature for 30min, then adding ethanol for rinsing twice, centrifuging and drying, and freezing and storing extracted DNA. Taking 2 microliter of the refrigerating fluid as a template, adding a primer buffer solution, Taq enzyme and MgSO4, supplementing a 25 microliter system with ultrapure water, carrying out PCR amplification, using 1.2% agarose gel for PCR reaction products, and adding a proper amount of dye GoldView according to the size of the gel. Electrophoresis was performed in an electrophoresis apparatus and the results were observed on a gel imaging system. The nucleotide sequences of the primers are shown below:
CN265:5’-TGGGTGGTTTACCATTCAGT-3’(SEQ ID NO.1)；
CN266:5’-CGCGACTTC GCAACCCAT-3’(SEQ ID NO.2)。
seedlings which do not carry virus diseases such as citrus yellow dragon disease and the like through PCR detection are planted in an isolated mode in an insect-proof net room, and are fertilized and watered regularly, so that the good growth vigor of the mother plants is guaranteed.
The mother plant management measures are as follows: (1) fertilizing: 3kg of organic fertilizer is applied to each plant at the end of 12 months to the beginning of 1 month in winter every year, and 0.5-1 kg of compound fertilizer is applied to the plants 10-15 days before the tips of the spring, summer, autumn and winter are respectively placed. (2) Spraying the pesticide: insecticide and bactericide (mancozeb, mineral oil, spinosad and the like) are sprayed to clean the garden in winter every year, and abamectin, beta-cypermethrin, copper hydroxide and the like are sprayed before and after each shoot release to prevent and control pests such as citrus leaf miner, citrus whitefly, canker and the like. (3) Water spraying: and (4) sprinkling water according to the growth and weather conditions of the phyllanthus emblica, and combining fertilization to promote the growth of plants.
2. Construction of cutting garden
Constructing a pest-proof net room of a cuttage garden, dividing the cuttage garden into strip-shaped ridges to ensure a seedbed and a farm operation space, and reserving a shading net above the pest-proof net room to lean against transverse columns.
EXAMPLE 1 field shoot cuttage
Preparation of seedbed:
soil (loess or field mud) with the thickness of 35-40 cm and the width of 0.8-1 m is paved on the strip-shaped ridges in the cutting garden, and the strips are covered with thicker plastic film paper for 5-10 days for high-temperature sterilization.
1) Preparing cuttage branches and cuttage
And cutting the peripheral aged branches of the mother plants after the shoots are placed, and cutting the branches to be 10-15 cm long, wherein two leaves at the middle upper part of each branch are reserved.
2) Bundling 50-100 branches into a bundle, and soaking the bundled branches for 8-10 min. And (3) after the prepared seedbed is thoroughly drenched, inserting the soaked tea branch and citrus reticulata branch into the seedbed according to the row spacing of 8-10 cm and the plant spacing of 8-10 cm, inserting the branch into the seedbed with the depth of 3-6 cm, keeping the leaves upward, and cutting without reverse insertion. And then inserting a bamboo strip with the length of 2-2.5 m into one side of the seedbed, inserting the bamboo strip into the seedbed with the depth of 8-10 cm, bending the bamboo strip into an arch shape, inserting the bamboo strip into the other side of the seedbed for fixing, covering thick white transparent plastic film paper on the bamboo strip, and uniformly pressing the periphery of the arch shed to prevent the plastic film from being opened.
3) Post-cuttage management
Spraying the surface of the seedbed once every 2-4 days after cuttage, and spraying broad-spectrum bactericides such as mancozeb, chlorothalonil and copper preparations in a penetrating manner and spraying water in the seedbed every 7-10 days. The temperature in the greenhouse is kept at 15-30 ℃, and the humidity is 70-90%.
4) After tender shoots of branches of the tea branches after cuttage are extracted, spraying an amino acid foliar fertilizer once every other week until the first shoots are aged, then spraying the amino acid foliar fertilizer once every other week and spraying a high-nitrogen compound fertilizer between two rows of seedlings until the second shoots are aged. If the temperature is high, opening a shading net at the top of the nursery to cool, and properly opening some openings of the covered film for ventilation and cooling; and if the weather is hot and humid, rolling up the two ends of the plastic film for ventilation.
5) Bagging and transplanting cutting seedlings
And (3) drenching the cutting seedlings aged for the second time in the step 4) with water, digging out roots of the cutting seedlings with soil after 1-2 days, and transplanting the roots into seedling bags with the diameter of 15-20 cm, wherein the seedling bags are provided with seedling breeding substrates which comprise 8-10 parts of coconut peat soil and 1-1.5 parts of compound fertilizer. Pouring water thoroughly, and building a shading net above.
6) Planting for seedling emergence and outplanting
And (3) performing spot inspection on leaves of the bred seedlings with good growth conditions, grinding, extracting DNA, detecting whether the leaves carry the yellow dragon citrus germs, and after detecting that the leaves are healthy and nontoxic, cutting the seedlings out of the nursery for planting.
Fig. 1 to 4 are schematic diagrams of the cutting method of the present invention. The required cutting seedbed is a strip-shaped bed paved by using field mud with good quality, the bed is uniformly sprayed with water after insolation and sterilization, and then tea branches and citrus branches are cut, so that the growth state of the cut tea branches and citrus branches is good.
EXAMPLE 2 cup seedling cuttage
Preparation of the matrix:
piling high-quality clay together, covering a thicker plastic film paper, and sealing a cover for 5-10 days for high-temperature sterilization. Then, high-quality soil is filled into seedling raising cups with the height of 5-10 cm, 4/5 with the cups being full is preferably used, the seedling raising cups are compactly placed in a cutting garden in a strip shape with the width of 1-1.5 m, and water is thoroughly sprayed.
1) Preparing cuttage branches and cuttage
And (3) cutting the mature shoot at the periphery of the mother plant to branches with the length of 10-15 cm, and reserving two leaves at the middle upper part of each branch.
2) Bundling 50-100 citrus reticulata branches into a bundle, and soaking the bundled branches for 8-10 min. During cuttage, one branch is planted into one cup. After cuttage, bamboo chips with the thickness of 2-2.5 m are inserted into two sides of the cuttage cup to form an arch, and the arch is covered by thick plastic film paper.
3) Spraying the surface of the seedbed once every 1-3 days after cuttage, and spraying a broad-spectrum bactericide such as mancozeb, chlorothalonil, a copper preparation and the like on the seedbed thoroughly and every 5-8 days. The temperature in the greenhouse is kept at 15-30 ℃, and the humidity is 70-90%.
4) After young buds of the cuttage tea branch citrus seedlings are extracted, amino acid foliar fertilizer is sprayed until the first tip is aged, and high nitrogen compound fertilizer is sprayed until the second tip is aged. If the temperature is high, the shading net on the top is opened to dissipate heat, and the covering film is properly opened to ventilate and cool.
5) Bagging and transplanting cutting seedlings
And (3) drenching the cuttage seedlings after the second shoot aging in the step 4) thoroughly, taking out the cuttage cup seedlings after 1-2 days, cutting off the outer bags, transplanting the cuttage seedlings into a new big bag together with the matrix, drenching thoroughly, and putting up a shading net above the cuttage cup seedlings.
6) Planting for seedling emergence and outplanting
And (4) performing spot inspection on the nursery stocks with good growth conditions, and taking out the cutting seedlings which do not carry the pholiota citriodora germs out of the nursery for planting after the detection is qualified.
Comparative example seedling of circled branches
Breeding time: 3 month
Selecting a mother plant and a branch: selecting plants with good growth conditions and luxuriant leaves as stock plants, and selecting robust and disease-free branches on the stock plants as ring branches.
And (3) branch circling operation: after the branch is selected, a knife is used for cutting the two circular cuts, and then the skin of the annular part is removed to expose the xylem. Then mixing the rice straws and the mud which are soaked in advance, kneading the mixture into a ball, surrounding the ball on the ring opening from bottom to top, tightly wrapping the ball into an olive shape, and tightly sealing the outer layer by using a plastic film.
Seedling falling: after 60 days, cutting off the seedlings with white roots in the film, removing the film, dipping the roots of the seedlings with slurry again to form a layer of slurry, and placing the seedlings in a shade place.
Temporary planting: transplanting the fallen seedlings on the prepared soil ridges, wherein the planting distance is 15 x 15cm, and thoroughly watering. The upper part is combined with the shading net. And then watering and fertilizing according to the seedling condition, soil and weather condition.
And (4) outplanting: outplanting after 3-4 months.
TABLE 1 comparison of the incidence of yellow shoot disease in the inventive cuttings and seedlings of the circulant
According to the table 1, the cutting seedlings can ensure that 100% of the citrus reticulata seedlings do not carry citrus yellow shoot germs when the cutting seedlings are out of the nursery, but the circled seedlings cannot be ensured when the cutting seedlings are out of the nursery, and the cutting seedlings are healthy and are planted around the orchard to isolate trees to prevent external psyllids, so that the incidence rate of citrus yellow shoot is still low after one year or two years of planting, and the release rate of the circled seedlings is high in the second year.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
SEQUENCE LISTING
<110> research institute of fruit trees of Guangdong province academy of agricultural sciences
<120> asexual propagation method of phyllanthus emblica
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<170>PatentIn version 3.5
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<213> Artificial sequence
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tgggtggttt accattcagt 20
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<212>DNA
<213> Artificial sequence
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cgcgacttcg caacccat 18
Claims (9)
1. A vegetative propagation method of phyllanthus emblica is characterized by comprising the following steps:
1) cutting off the peripheral mature branches in the season after the mother plant of the phyllanthus emblica is placed at the tip, and cutting the branches, wherein the branches keep two leaves at the middle upper part;
2) bundling the citrus reticulata branch into a bundle, soaking the bundle in a prepared rooting powder solution, taking out the bundle, then cutting the bundle to a seedbed, inserting bamboo chips on one side of the seedbed, bending the bamboo chips into an arch shape, inserting the bamboo chips into soil of the seedbed, fixing the bamboo chips, and covering the arched bamboo chips with a plastic film;
3) after cuttage, uniformly and thoroughly spraying the seedbed and spraying a bactericide;
4) after tender shoots of branches of the tea oranges are extracted, spraying an amino acid foliar fertilizer once every other week until the first shoots are aged, then spraying a foliar fertilizer once every other week, and broadcasting a high-nitrogen compound fertilizer between two rows of seedlings and spraying water until the second shoots are aged; preferably, in hot and humid days, the two ends of the plastic film are rolled up for ventilation;
5) and (4) watering the second-time-tip-aged cuttage seedlings thoroughly, then transplanting the seedlings into a seedling bag filled with a breeding matrix, and watering and shading.
2. The method as claimed in claim 1, further comprising the steps of spot-inspecting the leaves of the bred seedlings, grinding, extracting DNA, detecting whether the seedlings carry pathogenic bacteria of virulent infectious diseases, detecting that the seedlings carry the pathogenic bacteria positively, and performing outplanting planting on the healthy seedlings of the citrus chazus when the seedlings carry the pathogenic bacteria negatively.
3. The method of claim 2, wherein the virulent infectious disease is citrus yellow dragon.
4. The method according to claim 1, wherein the aged shoots of step 1) are aged shoots 15-20 days after shoot release in season; preferably, when the branches in the season are mature, the branches are cut into branches of 10-15 cm.
5. The method as claimed in claim 1, wherein the seedbed of step 2) is a strip-shaped bed, and soil with the thickness of 30-50 cm and the width of 1-1.5 m is paved on the bed.
6. The method according to claim 1, wherein step 2) comprises bundling 50 to 100 citrus chaenomeles branches into a bundle.
7. The method according to claim 1, wherein the citrus reticulata in step 2) is cut to a depth of 3-6 cm in a seedbed, the row spacing of the citrus reticulata cutting is 8-10 cm, and the plant spacing is 8-10 cm; preferably, the bamboo chips are inserted into the seedbed from one side to 8-10 cm deep, bent into an arch shape and then inserted into the seedbed soil on the other side for fixing.
8. The method of claim 1, wherein the germicide of step 3) is a broad spectrum germicide; the broad-spectrum bactericide is mancozeb, chlorothalonil and/or a copper preparation.
9. The method as claimed in claim 1, wherein the breeding substrate of step 5) comprises 10 parts of coconut coir peat soil mixture and 1 part of compound fertilizer or other mixture ratio according to fertilizer property.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111264199A (en) * | 2020-02-24 | 2020-06-12 | 江门市润之林生态农业科技有限公司 | Xinhui tea branch citrus cutting planting method |
| CN113330929A (en) * | 2021-02-24 | 2021-09-03 | 林为钦 | Citrus seedling growing method |
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| CN113330929A (en) * | 2021-02-24 | 2021-09-03 | 林为钦 | Citrus seedling growing method |
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