CN110770354B - 用于文库构建和序列分析的组合物和方法 - Google Patents

用于文库构建和序列分析的组合物和方法 Download PDF

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CN110770354B
CN110770354B CN201880040459.0A CN201880040459A CN110770354B CN 110770354 B CN110770354 B CN 110770354B CN 201880040459 A CN201880040459 A CN 201880040459A CN 110770354 B CN110770354 B CN 110770354B
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dna
chile
person
sequence
stranded
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CN110770354A (zh
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杰弗里·A·戈尔
阿瑟瓦·高赫
刘蕊
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Wuyuan Gene Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Structural Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Polyurethanes Or Polyureas (AREA)
  • Peptides Or Proteins (AREA)
CN201880040459.0A 2017-04-19 2018-04-18 用于文库构建和序列分析的组合物和方法 Active CN110770354B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762487423P 2017-04-19 2017-04-19
US62/487,423 2017-04-19
US201862657544P 2018-04-13 2018-04-13
US62/657,544 2018-04-13
PCT/US2018/028191 WO2018195217A1 (en) 2017-04-19 2018-04-18 Compositions and methods for library construction and sequence analysis

Publications (2)

Publication Number Publication Date
CN110770354A CN110770354A (zh) 2020-02-07
CN110770354B true CN110770354B (zh) 2024-03-19

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US (1) US11965157B2 (https=)
EP (1) EP3612641A4 (https=)
JP (1) JP7220200B2 (https=)
KR (1) KR102601593B1 (https=)
CN (1) CN110770354B (https=)
AU (1) AU2018256387A1 (https=)
CA (1) CA3060555A1 (https=)
SG (2) SG11201909618TA (https=)
TW (1) TWI797118B (https=)
WO (1) WO2018195217A1 (https=)

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US12234514B2 (en) 2018-12-21 2025-02-25 Grail, Inc. Source of origin deconvolution based on methylation fragments in cell-free DNA samples
CN112080555A (zh) * 2019-06-14 2020-12-15 上海鹍远健康科技有限公司 Dna甲基化检测试剂盒及检测方法
CN110964782A (zh) * 2019-12-09 2020-04-07 上海鹍远健康科技有限公司 单链核酸连接效率检测方法
CN111321208B (zh) * 2020-02-14 2023-10-03 上海厦维医学检验实验室有限公司 一种基于高通量测序的建库方法
BR112021026795A2 (pt) * 2020-02-26 2022-02-15 Illumina Cambridge Ltd Kits para genotipagem
CN111979583B (zh) * 2020-09-10 2023-09-12 杭州求臻医学检验实验室有限公司 一种单链核酸分子高通量测序文库的构建方法及其应用
CN114250298B (zh) * 2020-09-23 2024-09-24 中国医学科学院北京协和医院 胰腺导管腺癌的dna甲基化标志物及其应用
WO2022181858A1 (ko) * 2021-02-26 2022-09-01 지니너스 주식회사 분자 바코딩 효율을 향상시키기 위한 조성물 및 이의 용도
KR102924534B1 (ko) 2021-02-26 2026-02-06 지니너스 주식회사 분자 바코딩 효율을 향상시키기 위한 조성물 및 이의 용도
KR102695246B1 (ko) * 2021-11-25 2024-08-14 안신애 유전체와 후성 유전체 동시 분석 방법 및 분석 시스템
TWI878653B (zh) * 2022-03-03 2025-04-01 宏碁股份有限公司 核醣體rna資料庫的建構方法
US20250297406A1 (en) * 2022-04-18 2025-09-25 Chengdu Boe Optoelectronics Technology Co., Ltd. Adapters, adapter ligation reagent, kit, method for constructing dna library and method for sequencing gene
WO2024015869A2 (en) * 2022-07-12 2024-01-18 University Of Washington Systems and methods for variant detection in cells
KR102892827B1 (ko) * 2022-12-08 2025-12-02 주식회사 아이온셀 Dna 단편의 멀티 결합 조립 반응을 이용한 단일세포의 다중체 전장 시퀀싱 분석방법
WO2025117738A1 (en) * 2023-11-28 2025-06-05 Illumina, Inc. Methods of improving unique molecular index ligation efficiency

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Also Published As

Publication number Publication date
WO2018195217A1 (en) 2018-10-25
CN110770354A (zh) 2020-02-07
AU2018256387A1 (en) 2019-10-31
TWI797118B (zh) 2023-04-01
EP3612641A4 (en) 2021-01-20
SG10202111266VA (en) 2021-11-29
CA3060555A1 (en) 2018-10-25
JP2020517298A (ja) 2020-06-18
KR102601593B1 (ko) 2023-11-13
TW201842189A (zh) 2018-12-01
US11965157B2 (en) 2024-04-23
KR20190140961A (ko) 2019-12-20
JP7220200B2 (ja) 2023-02-09
SG11201909618TA (en) 2019-11-28
EP3612641A1 (en) 2020-02-26
US20200123538A1 (en) 2020-04-23

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