AU2018256387A1 - Compositions and methods for library construction and sequence analysis - Google Patents
Compositions and methods for library construction and sequence analysis Download PDFInfo
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- AU2018256387A1 AU2018256387A1 AU2018256387A AU2018256387A AU2018256387A1 AU 2018256387 A1 AU2018256387 A1 AU 2018256387A1 AU 2018256387 A AU2018256387 A AU 2018256387A AU 2018256387 A AU2018256387 A AU 2018256387A AU 2018256387 A1 AU2018256387 A1 AU 2018256387A1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
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- C—CHEMISTRY; METALLURGY
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
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- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
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| DE202019005627U1 (de) | 2018-04-02 | 2021-05-31 | Grail, Inc. | Methylierungsmarker und gezielte Methylierungssondenpanels |
| US20210164048A1 (en) * | 2018-08-07 | 2021-06-03 | Singlera Genomics, Inc. | A non-invasive prenatal test with accurate fetal fraction measurement |
| WO2020069350A1 (en) | 2018-09-27 | 2020-04-02 | Grail, Inc. | Methylation markers and targeted methylation probe panel |
| US12234514B2 (en) | 2018-12-21 | 2025-02-25 | Grail, Inc. | Source of origin deconvolution based on methylation fragments in cell-free DNA samples |
| CN112080555A (zh) * | 2019-06-14 | 2020-12-15 | 上海鹍远健康科技有限公司 | Dna甲基化检测试剂盒及检测方法 |
| CN110964782A (zh) * | 2019-12-09 | 2020-04-07 | 上海鹍远健康科技有限公司 | 单链核酸连接效率检测方法 |
| CN111321208B (zh) * | 2020-02-14 | 2023-10-03 | 上海厦维医学检验实验室有限公司 | 一种基于高通量测序的建库方法 |
| BR112021026795A2 (pt) * | 2020-02-26 | 2022-02-15 | Illumina Cambridge Ltd | Kits para genotipagem |
| CN111979583B (zh) * | 2020-09-10 | 2023-09-12 | 杭州求臻医学检验实验室有限公司 | 一种单链核酸分子高通量测序文库的构建方法及其应用 |
| CN114250298B (zh) * | 2020-09-23 | 2024-09-24 | 中国医学科学院北京协和医院 | 胰腺导管腺癌的dna甲基化标志物及其应用 |
| WO2022181858A1 (ko) * | 2021-02-26 | 2022-09-01 | 지니너스 주식회사 | 분자 바코딩 효율을 향상시키기 위한 조성물 및 이의 용도 |
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| TWI878653B (zh) * | 2022-03-03 | 2025-04-01 | 宏碁股份有限公司 | 核醣體rna資料庫的建構方法 |
| US20250297406A1 (en) * | 2022-04-18 | 2025-09-25 | Chengdu Boe Optoelectronics Technology Co., Ltd. | Adapters, adapter ligation reagent, kit, method for constructing dna library and method for sequencing gene |
| WO2024015869A2 (en) * | 2022-07-12 | 2024-01-18 | University Of Washington | Systems and methods for variant detection in cells |
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| US20150011396A1 (en) * | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| US20140093873A1 (en) | 2012-07-13 | 2014-04-03 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| US10482994B2 (en) | 2012-10-04 | 2019-11-19 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US10179933B2 (en) | 2013-02-07 | 2019-01-15 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Hybrid nanopores and uses thereof for detection of analytes |
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| CN120700143A (zh) | 2014-03-31 | 2025-09-26 | 梅奥医学教育和研究基金会 | 检测结直肠赘生物 |
| CA2951141C (en) | 2014-06-04 | 2021-11-30 | Quest Diagnostics Investments Incorporated | Methylated markers for colorectal cancer |
| JP6810612B2 (ja) | 2014-06-05 | 2021-01-06 | クリニカル ジェノミクス ピーティーワイ リミテッド | メチル化分析のための方法 |
| US10480021B2 (en) | 2014-06-23 | 2019-11-19 | Yale University | Methods for closed chromatin mapping and DNA methylation analysis for single cells |
| US11783911B2 (en) | 2014-07-30 | 2023-10-10 | Sequenom, Inc | Methods and processes for non-invasive assessment of genetic variations |
| JP6769969B2 (ja) * | 2015-01-12 | 2020-10-14 | １０エックス ジェノミクス， インコーポレイテッド | 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー |
| US9984201B2 (en) | 2015-01-18 | 2018-05-29 | Youhealth Biotech, Limited | Method and system for determining cancer status |
| WO2016172442A1 (en) | 2015-04-23 | 2016-10-27 | Quest Diagnostics Investments Incorporated | Mlh1 methylation assay |
| US20170101674A1 (en) * | 2015-08-21 | 2017-04-13 | Toma Biosciences, Inc. | Methods, compositions, and kits for nucleic acid analysis |
| GB201515557D0 (en) | 2015-09-02 | 2015-10-14 | 14M Genomics Ltd | Method of sequencing |
| HUE050491T2 (hu) | 2015-11-10 | 2020-12-28 | Eurofins Lifecodexx Gmbh | Magzati kromoszomális aneuploidiák kimutatása olyan DNS régiókat alkalmazva, amelyek különbözõképpen vannak metilezve a magzat és a terhes nõstény között |
| KR102006803B1 (ko) | 2016-10-06 | 2019-08-05 | (주)지노믹트리 | 메틸화 dna 다중 검출방법 |
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| EP3612641A1 (en) | 2020-02-26 |
| US20200123538A1 (en) | 2020-04-23 |
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