CA3060555A1 - Compositions and methods for library construction and sequence analysis - Google Patents
Compositions and methods for library construction and sequence analysis Download PDFInfo
- Publication number
- CA3060555A1 CA3060555A1 CA3060555A CA3060555A CA3060555A1 CA 3060555 A1 CA3060555 A1 CA 3060555A1 CA 3060555 A CA3060555 A CA 3060555A CA 3060555 A CA3060555 A CA 3060555A CA 3060555 A1 CA3060555 A1 CA 3060555A1
- Authority
- CA
- Canada
- Prior art keywords
- seq
- sequence
- stranded
- ligation
- library
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Structural Engineering (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Polyurethanes Or Polyureas (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762487423P | 2017-04-19 | 2017-04-19 | |
| US62/487,423 | 2017-04-19 | ||
| US201862657544P | 2018-04-13 | 2018-04-13 | |
| US62/657,544 | 2018-04-13 | ||
| PCT/US2018/028191 WO2018195217A1 (en) | 2017-04-19 | 2018-04-18 | Compositions and methods for library construction and sequence analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3060555A1 true CA3060555A1 (en) | 2018-10-25 |
Family
ID=63856830
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3060555A Pending CA3060555A1 (en) | 2017-04-19 | 2018-04-18 | Compositions and methods for library construction and sequence analysis |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US11965157B2 (https=) |
| EP (1) | EP3612641A4 (https=) |
| JP (1) | JP7220200B2 (https=) |
| KR (1) | KR102601593B1 (https=) |
| CN (1) | CN110770354B (https=) |
| AU (1) | AU2018256387A1 (https=) |
| CA (1) | CA3060555A1 (https=) |
| SG (2) | SG11201909618TA (https=) |
| TW (1) | TWI797118B (https=) |
| WO (1) | WO2018195217A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979583A (zh) * | 2020-09-10 | 2020-11-24 | 杭州求臻医学检验实验室有限公司 | 一种单链核酸分子高通量测序文库的构建方法及其应用 |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2019234843B2 (en) | 2018-03-13 | 2025-12-04 | Grail, Inc. | Anomalous fragment detection and classification |
| DE202019005627U1 (de) | 2018-04-02 | 2021-05-31 | Grail, Inc. | Methylierungsmarker und gezielte Methylierungssondenpanels |
| US20210164048A1 (en) * | 2018-08-07 | 2021-06-03 | Singlera Genomics, Inc. | A non-invasive prenatal test with accurate fetal fraction measurement |
| WO2020069350A1 (en) | 2018-09-27 | 2020-04-02 | Grail, Inc. | Methylation markers and targeted methylation probe panel |
| US12234514B2 (en) | 2018-12-21 | 2025-02-25 | Grail, Inc. | Source of origin deconvolution based on methylation fragments in cell-free DNA samples |
| CN112080555A (zh) * | 2019-06-14 | 2020-12-15 | 上海鹍远健康科技有限公司 | Dna甲基化检测试剂盒及检测方法 |
| CN110964782A (zh) * | 2019-12-09 | 2020-04-07 | 上海鹍远健康科技有限公司 | 单链核酸连接效率检测方法 |
| CN111321208B (zh) * | 2020-02-14 | 2023-10-03 | 上海厦维医学检验实验室有限公司 | 一种基于高通量测序的建库方法 |
| BR112021026795A2 (pt) * | 2020-02-26 | 2022-02-15 | Illumina Cambridge Ltd | Kits para genotipagem |
| CN114250298B (zh) * | 2020-09-23 | 2024-09-24 | 中国医学科学院北京协和医院 | 胰腺导管腺癌的dna甲基化标志物及其应用 |
| WO2022181858A1 (ko) * | 2021-02-26 | 2022-09-01 | 지니너스 주식회사 | 분자 바코딩 효율을 향상시키기 위한 조성물 및 이의 용도 |
| KR102924534B1 (ko) | 2021-02-26 | 2026-02-06 | 지니너스 주식회사 | 분자 바코딩 효율을 향상시키기 위한 조성물 및 이의 용도 |
| KR102695246B1 (ko) * | 2021-11-25 | 2024-08-14 | 안신애 | 유전체와 후성 유전체 동시 분석 방법 및 분석 시스템 |
| TWI878653B (zh) * | 2022-03-03 | 2025-04-01 | 宏碁股份有限公司 | 核醣體rna資料庫的建構方法 |
| US20250297406A1 (en) * | 2022-04-18 | 2025-09-25 | Chengdu Boe Optoelectronics Technology Co., Ltd. | Adapters, adapter ligation reagent, kit, method for constructing dna library and method for sequencing gene |
| WO2024015869A2 (en) * | 2022-07-12 | 2024-01-18 | University Of Washington | Systems and methods for variant detection in cells |
| KR102892827B1 (ko) * | 2022-12-08 | 2025-12-02 | 주식회사 아이온셀 | Dna 단편의 멀티 결합 조립 반응을 이용한 단일세포의 다중체 전장 시퀀싱 분석방법 |
| WO2025117738A1 (en) * | 2023-11-28 | 2025-06-05 | Illumina, Inc. | Methods of improving unique molecular index ligation efficiency |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005040399A2 (en) | 2003-10-21 | 2005-05-06 | Orion Genomics Llc | Methods for quantitative determination of methylation density in a dna locus |
| EA013634B1 (ru) | 2005-04-15 | 2010-06-30 | Эпиджиномикс Аг | Способы и нуклеиновые кислоты для анализов клеточных пролиферативных нарушений |
| WO2006113770A1 (en) | 2005-04-15 | 2006-10-26 | Epigenomics Ag | A method for providing dna fragments derived from a remote sample |
| US7582405B2 (en) | 2005-10-26 | 2009-09-01 | Hewlett-Packard Development Company, L.P. | Image recording media and image layers |
| GB0522310D0 (en) | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| US7901882B2 (en) | 2006-03-31 | 2011-03-08 | Affymetrix, Inc. | Analysis of methylation using nucleic acid arrays |
| US20080261217A1 (en) | 2006-10-17 | 2008-10-23 | Melnikov Anatoliy A | Methylation Profile of Cancer |
| EP2542698B1 (en) | 2010-03-03 | 2015-04-22 | Zymo Research Corporation | Detection of dna methylation |
| CA2826696C (en) | 2011-02-02 | 2019-12-03 | Mayo Foundation For Medical Education And Research | Digital sequence analysis of dna methylation |
| NO2729579T3 (https=) | 2011-07-08 | 2018-03-03 | ||
| EP4001436B1 (en) | 2012-05-11 | 2024-03-13 | Clinical Genomics Pty. Ltd. | Diagnostic gene marker panel |
| US20150011396A1 (en) * | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| US20140093873A1 (en) | 2012-07-13 | 2014-04-03 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| US10482994B2 (en) | 2012-10-04 | 2019-11-19 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
| US10179933B2 (en) | 2013-02-07 | 2019-01-15 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Hybrid nanopores and uses thereof for detection of analytes |
| EP2959016A4 (en) * | 2013-02-21 | 2016-10-12 | Toma Biosciences Inc | METHOD, COMPOSITIONS AND NUCLEIC ACID ANALYSIS KITS |
| ES2746237T3 (es) | 2013-03-14 | 2020-03-05 | Univ Cornell | Método para la cuantificación relativa de cambios en la metilación del ADN, usando reacciones combinadas de nucleasa, ligamiento, y polimerasa |
| CN120700143A (zh) | 2014-03-31 | 2025-09-26 | 梅奥医学教育和研究基金会 | 检测结直肠赘生物 |
| CA2951141C (en) | 2014-06-04 | 2021-11-30 | Quest Diagnostics Investments Incorporated | Methylated markers for colorectal cancer |
| JP6810612B2 (ja) | 2014-06-05 | 2021-01-06 | クリニカル ジェノミクス ピーティーワイ リミテッド | メチル化分析のための方法 |
| US10480021B2 (en) | 2014-06-23 | 2019-11-19 | Yale University | Methods for closed chromatin mapping and DNA methylation analysis for single cells |
| US11783911B2 (en) | 2014-07-30 | 2023-10-10 | Sequenom, Inc | Methods and processes for non-invasive assessment of genetic variations |
| JP6769969B2 (ja) * | 2015-01-12 | 2020-10-14 | １０エックス ジェノミクス， インコーポレイテッド | 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー |
| US9984201B2 (en) | 2015-01-18 | 2018-05-29 | Youhealth Biotech, Limited | Method and system for determining cancer status |
| WO2016172442A1 (en) | 2015-04-23 | 2016-10-27 | Quest Diagnostics Investments Incorporated | Mlh1 methylation assay |
| US20170101674A1 (en) * | 2015-08-21 | 2017-04-13 | Toma Biosciences, Inc. | Methods, compositions, and kits for nucleic acid analysis |
| GB201515557D0 (en) | 2015-09-02 | 2015-10-14 | 14M Genomics Ltd | Method of sequencing |
| HUE050491T2 (hu) | 2015-11-10 | 2020-12-28 | Eurofins Lifecodexx Gmbh | Magzati kromoszomális aneuploidiák kimutatása olyan DNS régiókat alkalmazva, amelyek különbözõképpen vannak metilezve a magzat és a terhes nõstény között |
| KR102006803B1 (ko) | 2016-10-06 | 2019-08-05 | (주)지노믹트리 | 메틸화 dna 다중 검출방법 |
| CA3060553A1 (en) | 2017-04-19 | 2018-10-25 | Singlera Genomics, Inc. | Compositions and methods for detection of genomic variance and dna methylation status |
-
2018
- 2018-04-18 WO PCT/US2018/028191 patent/WO2018195217A1/en not_active Ceased
- 2018-04-18 SG SG11201909618T patent/SG11201909618TA/en unknown
- 2018-04-18 KR KR1020197033449A patent/KR102601593B1/ko active Active
- 2018-04-18 SG SG10202111266VA patent/SG10202111266VA/en unknown
- 2018-04-18 CA CA3060555A patent/CA3060555A1/en active Pending
- 2018-04-18 CN CN201880040459.0A patent/CN110770354B/zh active Active
- 2018-04-18 JP JP2020507488A patent/JP7220200B2/ja active Active
- 2018-04-18 AU AU2018256387A patent/AU2018256387A1/en not_active Abandoned
- 2018-04-18 US US16/605,203 patent/US11965157B2/en active Active
- 2018-04-18 EP EP18788271.7A patent/EP3612641A4/en active Pending
- 2018-04-19 TW TW107113306A patent/TWI797118B/zh active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979583A (zh) * | 2020-09-10 | 2020-11-24 | 杭州求臻医学检验实验室有限公司 | 一种单链核酸分子高通量测序文库的构建方法及其应用 |
| CN111979583B (zh) * | 2020-09-10 | 2023-09-12 | 杭州求臻医学检验实验室有限公司 | 一种单链核酸分子高通量测序文库的构建方法及其应用 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2018195217A1 (en) | 2018-10-25 |
| CN110770354A (zh) | 2020-02-07 |
| AU2018256387A1 (en) | 2019-10-31 |
| TWI797118B (zh) | 2023-04-01 |
| EP3612641A4 (en) | 2021-01-20 |
| SG10202111266VA (en) | 2021-11-29 |
| JP2020517298A (ja) | 2020-06-18 |
| KR102601593B1 (ko) | 2023-11-13 |
| TW201842189A (zh) | 2018-12-01 |
| CN110770354B (zh) | 2024-03-19 |
| US11965157B2 (en) | 2024-04-23 |
| KR20190140961A (ko) | 2019-12-20 |
| JP7220200B2 (ja) | 2023-02-09 |
| SG11201909618TA (en) | 2019-11-28 |
| EP3612641A1 (en) | 2020-02-26 |
| US20200123538A1 (en) | 2020-04-23 |
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