CN110754588A - Seaweed functional feed suitable for grouper fries and preparation method thereof - Google Patents
Seaweed functional feed suitable for grouper fries and preparation method thereof Download PDFInfo
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- CN110754588A CN110754588A CN201911281877.1A CN201911281877A CN110754588A CN 110754588 A CN110754588 A CN 110754588A CN 201911281877 A CN201911281877 A CN 201911281877A CN 110754588 A CN110754588 A CN 110754588A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/153—Nucleic acids; Hydrolysis products or derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
Abstract
The invention provides a seaweed functional feed suitable for grouper fries and a preparation method thereof, wherein the seaweed functional feed comprises the following raw materials in parts by weight: 38-58 parts of a compound seaweed extract, 10-15 parts of taurine, 11-18 parts of glutamic acid, 12-21 parts of alanine, 10-20 parts of glycine, 14-19 parts of sodium inosinate and 2-8 parts of an antioxidant, wherein the compound seaweed extract is prepared from the following components in parts by weight: 2-5: 1.2-2.6, the seaweed functional feed applicable to the epinephelus coioides fingerlings can change the palatability of the feed, can obviously improve the anti-stress capability and growth speed of the epinephelus coioides fingerlings while enhancing the food calling property, improves the survival rate, ingestion rate and growth rate of the epinephelus coioides fingerlings, has theoretical and practical guiding significance for artificial breeding technology of the epinephelus coioides fingerlings and improvement of the breeding economic benefits of the epinephelus coioides fingerlings, lays a technical foundation for large-scale breeding of the epinephelus coioides fingerlings, and has great social and economic benefits.
Description
Technical Field
The invention relates to the field of aquaculture, and in particular relates to a seaweed functional feed suitable for grouper fries and a preparation method thereof.
Background
Grouper is a general name of fishes in the subfamily grouper (Epinephalae) and belongs to Osteichthyes, nephridae, Acanthopterygii, Perciformes, Percoidei and Serranidae, is warm water large and medium-sized marine fishes and is widely distributed in tropical and subtropical sea areas, is mainly distributed in Taiwan strait and south sea areas in China, is fat and delicious and is popular aquatic fishes, and is fed with other chemical baits or other fish fries in the current grouper breeding process, so that under natural conditions, the survival rate of the grouper is low, the utilization rate of the grouper on the feed is low, the phenomenon of spitting is generated during feeding, the feed palatability is poor, and more flavor substances are generated, so that the appetite of the grouper is influenced, the feed intake is reduced, and the growth is hindered.
Disclosure of Invention
In view of the above, the invention provides a seaweed functional feed suitable for grouper fries and a preparation method thereof, and solves the problems.
The technical scheme of the invention is realized as follows: a seaweed functional feed suitable for grouper fries and a preparation method thereof are as follows:
the seaweed functional feed comprises the following raw materials in parts by weight: 38-58 parts of a compound seaweed extract, 10-15 parts of taurine, 11-18 parts of glutamic acid, 12-21 parts of alanine, 10-20 parts of glycine, 14-19 parts of sodium inosinate and 2-8 parts of an antioxidant, wherein the compound seaweed extract is prepared from the following components in parts by weight: 2-5: 1.2-2.6 microwave extracting seaweed, kaempferia galangal and gallnut;
further, the seaweed functional feed comprises the following raw materials in parts by weight: 48 parts of compound seaweed extract, 12 parts of taurine, 14 parts of glutamic acid, 17 parts of alanine, 15 parts of glycine, 16 parts of sodium inosinate and 5 parts of antioxidant, wherein the compound seaweed extract is prepared from the following components in parts by weight: 3: 2, performing microwave extraction on the seaweed, the kaempferia galangal and the gallnut;
further, the antioxidant is one or more of vitamin C, butyl hydroxy anisole and green tea polyphenol;
further, the preparation method of the seaweed functional feed comprises the following steps:
(1) seaweed concentrated solution: pulverizing seaweed, adding purified water 40-60 times of the seaweed, adjusting the pH value to 1-2 with hydrochloric acid or sulfuric acid, heating and extracting at 50-60 ℃ for 3-4 hours, separating filter residues to obtain a seaweed concentrated solution,
(2) compound seaweed extract: crushing the kaempferia galanga and gallnut, sieving with a 150-300-mesh sieve, grinding and homogenizing in a dilute acid solution with the pH value of 2-4, adding a seaweed concentrated solution, performing microwave extraction at the pH value of 5.0-5.5 at the microwave frequency of 3800-7500 MHz for 1-3 min, curing and sterilizing at the temperature of 121-135 ℃ and under the pressure of 1-5 MPa for 0.5-2 h, degrading, and adding sodium hydroxide to the degraded composite seaweed extract to adjust the pH value to 5.3-7.0 to obtain the composite seaweed extract;
(3) mixing and stirring the compound seaweed extract, taurine, glutamic acid, alanine, glycine and inosinic acid, adding an antioxidant, heating at the temperature of 60-120 ℃ for 0.5-2 hours, and then carrying out freeze drying to obtain the seaweed functional feed.
Compared with the prior art, the invention has the beneficial effects that:
the seaweed functional feed suitable for the epinephelus coioides fingerlings can change the palatability of the feed, improve the appetite of the epinephelus coioides fingerlings by odor attraction and taste perception to achieve the effects of promoting growth and shortening the culture period, shorten the retention time of the feed in water, play a certain role in improving water quality, obviously improve the anti-stress capability and the growth speed of the epinephelus coioides fingerlings while enhancing the food attraction, improve the survival rate, the ingestion rate and the growth rate of the epinephelus coioides fingerlings, wherein the seaweed retains the original fishy smell after being crushed, is combined with the rhizoma kaempferiae and the gallnut for extraction, has unique taste, can stimulate the taste and the smell of the epinephelus coioides fingerlings to play a good role in promoting the feeding, has rich amino acids, has good natural odor attraction to the epinephrine, and can be well fused with the composite seaweed extract by adding the antioxidant, the fish species growth promoting agent has the advantages that the growth of fish species and the activity of digestive enzymes and the like are promoted, the raw materials are scientifically matched, the optimal food calling and growth promoting effect is achieved, the artificial breeding technology of the grouper fish species and the improvement of the breeding economic benefit of the grouper fish species have theoretical and practical guiding significance, the technical foundation is laid for large-scale breeding, and the social and economic benefits are great.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
Example 1
The seaweed functional feed comprises the following raw materials in parts by weight: 38 parts of a compound seaweed extract, 10 parts of taurine, 11 parts of glutamic acid, 12 parts of alanine, 10 parts of glycine, 14 parts of sodium inosinate and 2 parts of vitamin C, wherein the compound seaweed extract is prepared from the following components in parts by weight: 2: 1.2 microwave extracting seaweed, kaempferia galangal and gallnut.
Example 2
The seaweed functional feed comprises the following raw materials in parts by weight: 58 parts of compound seaweed extract, 15 parts of taurine, 18 parts of glutamic acid, 21 parts of alanine, 20 parts of glycine, 19 parts of sodium inosinate and 8 parts of green tea polyphenol, wherein the compound seaweed extract is prepared from the following components in parts by weight: 5: 2.6 microwave extracting seaweed, rhizoma kaempferiae and Galla chinensis.
Example 3
The seaweed functional feed comprises the following raw materials in parts by weight: 48 parts of compound seaweed extract, 12 parts of taurine, 14 parts of glutamic acid, 17 parts of alanine, 15 parts of glycine, 16 parts of sodium inosinate and 5 parts of antioxidant, wherein the compound seaweed extract is prepared from the following components in parts by weight: 3: 2, performing microwave extraction on the seaweed, the kaempferia galangal and gallnut, wherein the antioxidant consists of vitamin C and butyl hydroxy anisol in parts by weight of 1-2;
the preparation method of the seaweed functional feed in the embodiments 1-3 comprises the following steps:
(1) seaweed concentrated solution: pulverizing Sargassum, adding 50 times of purified water, adjusting pH to 1.3 with hydrochloric acid or sulfuric acid, heating and extracting at 55 deg.C for 3.5 hr, separating residue to obtain Sargassum concentrated solution,
(2) compound seaweed extract: pulverizing rhizoma Kaempferiae and Galla chinensis, sieving with 200 mesh sieve, grinding in dilute acid solution with pH of 3, homogenizing, adding Sargassum concentrated solution, performing microwave extraction at pH of 5.0, microwave frequency of 5000MHz for 2min, aging and sterilizing at 128 deg.C and 3MPa for 1.5 hr, degrading, and adjusting pH to 6.1 with sodium hydroxide to obtain composite Sargassum extract;
(3) mixing and stirring the compound seaweed extract, taurine, glutamic acid, alanine, glycine and inosinic acid, adding the antioxidant, heating at 90 ℃ for 1.2 hours, and then carrying out freeze drying to obtain the seaweed functional feed.
Example 4
In this example, the same weight parts of raw materials as in example 3 were used, and the preparation method included the following steps:
(1) seaweed concentrated solution: pulverizing Sargassum, adding 40 times of purified water, adjusting pH to 1 with hydrochloric acid or sulfuric acid, heating and extracting at 50 deg.C for 3 hr, separating residue to obtain Sargassum concentrated solution,
(2) compound seaweed extract: pulverizing rhizoma Kaempferiae and Galla chinensis, sieving with 150 mesh sieve, grinding in dilute acid solution with pH of 2, homogenizing, adding Sargassum concentrated solution, performing microwave extraction at pH of 5.0, microwave frequency of 3800MHz for 1min, aging and sterilizing at 121 deg.C and 1MPa for 0.5 hr, degrading, and adding sodium hydroxide to adjust pH to 5.3 to obtain composite Sargassum extract;
(3) mixing and stirring the compound seaweed extract, taurine, glutamic acid, alanine, glycine and inosinic acid, adding the antioxidant, heating at 90 ℃ for 1.2 hours, and then carrying out freeze drying to obtain the seaweed functional feed.
Example 5
In this example, the same weight parts of raw materials as in example 3 were used, and the preparation method included the following steps:
(1) seaweed concentrated solution: pulverizing seaweed, adding purified water 40-60 times of the seaweed, adjusting pH to 2 with hydrochloric acid or sulfuric acid, heating and extracting at 60 deg.C for 4 hr, separating filter residue to obtain seaweed concentrated solution,
(2) compound seaweed extract: pulverizing rhizoma Kaempferiae and Galla chinensis, sieving with 300 mesh sieve, grinding in dilute acid solution with pH 4, homogenizing, adding concentrated Sargassum solution, performing microwave extraction at pH 5.5 with microwave frequency of 7500MHz for 3min, aging and sterilizing at 135 deg.C and 5MPa for 2 hr, degrading, and adjusting pH to 7.0 with sodium hydroxide to obtain composite Sargassum extract;
(3) mixing and stirring the compound seaweed extract, taurine, glutamic acid, alanine, glycine and inosinic acid, adding the antioxidant, heating at 90 ℃ for 1.5 hours, and then carrying out freeze drying to obtain the seaweed functional feed.
Example 6
This example differs from example 3 in that: compound seaweed extract: pulverizing rhizoma Kaempferiae and Galla chinensis, sieving with 200 mesh sieve, grinding in dilute acid solution with pH of 3, homogenizing, adding Sargassum concentrated solution, extracting with water vapor under reflux at pH of 5.0 for 3 times for 3 hr, aging and sterilizing at 128 deg.C and 3MPa for 1.5 hr, degrading, and adjusting pH to 6.1 with sodium hydroxide to obtain composite Sargassum extract.
Comparative example 1
This comparative example differs from example 3 in that: the seaweed functional feed comprises the following raw materials in parts by weight: the compound seaweed extract comprises, by weight, 30 parts of a compound seaweed extract, 12 parts of taurine, 10 parts of glutamic acid, 10 parts of alanine, 15 parts of glycine, 13 parts of sodium inosinate and 1 part of an antioxidant, wherein the compound seaweed extract is prepared from the following components in parts by weight: 3: 2 microwave extracting the seaweed, the kaempferia galangal and the gallnut.
Comparative example 2
This comparative example differs from example 3 in that: the seaweed functional feed comprises the following raw materials in parts by weight: 30 parts of compound seaweed extract, 12 parts of taurine, 14 parts of glutamic acid, 17 parts of alanine, 15 parts of glycine, 16 parts of sodium inosinate and 5 parts of antioxidant, wherein the compound seaweed extract is prepared from the following components in parts by weight: 3: 2 microwave extracting the seaweed, the kaempferia galangal and the gallnut.
Comparative example 3
This comparative example differs from example 3 in that: the seaweed functional feed comprises the following raw materials in parts by weight: 48 parts of compound seaweed extract, 12 parts of taurine, 14 parts of glutamic acid, 17 parts of alanine, 15 parts of glycine, 16 parts of sodium inosinate and 5 parts of antioxidant, wherein the compound seaweed extract is prepared from the following components in parts by weight: 1: 2 microwave extracting the seaweed, the kaempferia galangal and the gallnut.
First, growth index
Feeding the seaweed functional feeds prepared in the examples 1-6 and the comparative examples 1-3 with rockfish fingerlings respectively, feeding 3 times a day, 3-6% of the fish amount each time, after 4 weeks, carrying out starvation treatment on the fingerlings for 2 days, and collecting experimental data,
body length growth rate (%) [ (average end body length-average initial body length)/average initial body length ] × 100
The data are as follows:
as can be seen from the above table, in the embodiments 1 to 3 of the prepared seaweed functional feed, the raw materials are scientifically proportioned, the original fishy smell of the seaweed is kept after the seaweed is crushed, the seaweed is combined with the kaempferia galangal and the gallnut for extraction, the seaweed functional feed has special taste and can stimulate the taste and smell of the fingerlings, and the antioxidant is added to fuse the taurine, the glutamic acid, the alanine, the glycine and the inosinic acid with the composite seaweed extract so as to promote the growth of the fingerlings and the activities of digestive enzymes and the like; in examples 3 to 6, specific process parameters in the preparation method are adjusted to achieve the best food calling and growth promoting effect, the body length growth rate of example 3 is 2.94%, the ingestion rate is 98.3%, and the survival rate is 89.5%, and compared with comparative examples 1 to 3, the functional feed with high ingestion rate can be obtained according to the raw materials and the proportion of the invention.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. A seaweed functional feed suitable for grouper fries is characterized in that: the feed comprises the following raw materials in parts by weight: 38-58 parts of a compound seaweed extract, 10-15 parts of taurine, 11-18 parts of glutamic acid, 12-21 parts of alanine, 10-20 parts of glycine, 14-19 parts of sodium inosinate and 2-8 parts of an antioxidant, wherein the compound seaweed extract is prepared from the following components in parts by weight: 2-5: 1.2-2.6 microwave extracting seaweed, kaempferia galanga and gallnut.
2. The seaweed functional feed as claimed in claim 1, which is suitable for grouper fries, and is characterized in that: the feed comprises the following raw materials in parts by weight: 48 parts of compound seaweed extract, 12 parts of taurine, 14 parts of glutamic acid, 17 parts of alanine, 15 parts of glycine, 16 parts of sodium inosinate and 5 parts of antioxidant, wherein the compound seaweed extract is prepared from the following components in parts by weight: 3: 2 microwave extracting the seaweed, the kaempferia galangal and the gallnut.
3. The seaweed functional feed suitable for the epinephelus seedlings according to claim 1, wherein the antioxidant is one or more of vitamin C, butyl hydroxy anisole and green tea polyphenol.
4. The method for preparing the seaweed functional feed suitable for the grouper fries as claimed in claim 1, wherein the method comprises the following steps: the method comprises the following steps:
(1) seaweed concentrated solution: pulverizing seaweed, adding purified water 40-60 times of the seaweed, adjusting the pH value to 1-2 with hydrochloric acid or sulfuric acid, heating and extracting for 3-4 hours, separating filter residues to obtain a seaweed concentrated solution,
(2) compound seaweed extract: pulverizing the kaempferia galanga and the gallnut, sieving with a 150-300-mesh sieve, grinding and homogenizing in a dilute acid solution with the pH value of 2-4, adding a seaweed concentrated solution, performing microwave extraction for 1-3 min at the pH value of 5.0-5.5, curing and sterilizing at the temperature of 121-135 ℃ under the pressure of 1-5 MPa for 0.5-2 h, degrading, adding sodium hydroxide into the degraded composite seaweed extract to adjust the pH value to 5.3-7.0 to obtain the composite seaweed extract,
(3) mixing and stirring the compound seaweed extract, taurine, glutamic acid, alanine, glycine and inosinic acid, adding an antioxidant, heating at the temperature of 60-120 ℃ for 0.5-2 hours, and then carrying out freeze drying to obtain the seaweed functional feed.
5. The method for preparing the seaweed functional feed suitable for the grouper fries as claimed in claim 1, wherein the method comprises the following steps: in the extraction of the seaweed concentrated solution, the heating temperature is 50-60 ℃.
6. The method for preparing the seaweed functional feed suitable for the grouper fries as claimed in claim 1, wherein the method comprises the following steps: the microwave frequency is 3800-7500 MHz.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911281877.1A CN110754588A (en) | 2019-12-13 | 2019-12-13 | Seaweed functional feed suitable for grouper fries and preparation method thereof |
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| CN201911281877.1A CN110754588A (en) | 2019-12-13 | 2019-12-13 | Seaweed functional feed suitable for grouper fries and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772795A (en) * | 2021-01-29 | 2021-05-11 | 海南晨海水产有限公司 | Golden tiger spot multifunctional compound feed and preparation method thereof |
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| CN101744145A (en) * | 2008-12-19 | 2010-06-23 | 中国科学院海洋研究所 | Meat quality flavor modifying agent for grass carp or carp and preparation method thereof |
| CN103445013A (en) * | 2013-08-07 | 2013-12-18 | 利辛县心连心饲料有限责任公司 | Carp feed and preparation method thereof |
| CN104304785A (en) * | 2014-10-17 | 2015-01-28 | 中国水产科学研究院南海水产研究所 | Seaweed functional compound feed for garrupa of seedling stage |
| CN106901062A (en) * | 2015-12-22 | 2017-06-30 | 彭志成 | A kind of grouper phagostimulant |
| CN108157678A (en) * | 2017-12-29 | 2018-06-15 | 磐安县派普特生物科技有限公司 | A kind of preparation method containing Auricularia polysaccharide preparing grass carp feed additive |
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2019
- 2019-12-13 CN CN201911281877.1A patent/CN110754588A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5229118A (en) * | 1991-09-30 | 1993-07-20 | Aqua-10 Corporation | Feed and water additive and method of making same |
| CN101744145A (en) * | 2008-12-19 | 2010-06-23 | 中国科学院海洋研究所 | Meat quality flavor modifying agent for grass carp or carp and preparation method thereof |
| CN103445013A (en) * | 2013-08-07 | 2013-12-18 | 利辛县心连心饲料有限责任公司 | Carp feed and preparation method thereof |
| CN104304785A (en) * | 2014-10-17 | 2015-01-28 | 中国水产科学研究院南海水产研究所 | Seaweed functional compound feed for garrupa of seedling stage |
| CN106901062A (en) * | 2015-12-22 | 2017-06-30 | 彭志成 | A kind of grouper phagostimulant |
| CN108157678A (en) * | 2017-12-29 | 2018-06-15 | 磐安县派普特生物科技有限公司 | A kind of preparation method containing Auricularia polysaccharide preparing grass carp feed additive |
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| CN112772795A (en) * | 2021-01-29 | 2021-05-11 | 海南晨海水产有限公司 | Golden tiger spot multifunctional compound feed and preparation method thereof |
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Application publication date: 20200207 |