CN113796339A - Breeding method for improving heat stress resistance of Bass sand fish - Google Patents

Breeding method for improving heat stress resistance of Bass sand fish Download PDF

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Publication number
CN113796339A
CN113796339A CN202111001180.1A CN202111001180A CN113796339A CN 113796339 A CN113796339 A CN 113796339A CN 202111001180 A CN202111001180 A CN 202111001180A CN 113796339 A CN113796339 A CN 113796339A
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feeding
parts
fish
chinese medicine
weight
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陈恺
周国栋
蔡利
周国桓
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Hainan Tianjiao Technology Development Co ltd
Jiawei Biotechnology Co Ltd
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Hainan Tianjiao Technology Development Co ltd
Jiawei Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/10Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention provides a breeding method for improving heat stress resistance of basha fish, which comprises the following steps: feeding the traditional Chinese medicine additive in a culture period, wherein the daily feeding amount is 1.5-3.1% of the weight of the fish, and stopping feeding for 7-10 days after continuous feeding for 2-4 days; feeding the traditional Chinese medicine additive again, wherein the daily feeding amount is 1.8-2.5% of the weight of the fish, stopping feeding after 2-4 days of continuous feeding, and naturally feeding; the traditional Chinese medicine additive is prepared from the following raw materials in parts by weight: 50-80 parts of hawthorn, 100 parts of dried orange peel, 150 parts of isatis root, 70-100 parts of rhizoma atractylodis and 50-80 parts of gentian. The method for breeding the basha fish effectively improves the heat stress resistance of the basha fish, enables the basha fish to adapt to climate change better, effectively improves the survival rate of the basha fish, also effectively improves the growth speed of the basha fish, ensures the quality of the basha fish more effectively and improves the economic benefit.

Description

Breeding method for improving heat stress resistance of Bass sand fish
Technical Field
The invention relates to the field of aquaculture, in particular to a culture method for improving heat stress resistance of basha fish.
Background
The Pangarius bocourti Sauvage belongs to the order Parasilurus in Clariales, and the family Parasilurus. The Pacific saury is high-quality economic fish. The barbeque has the advantages of white and tender meat, delicious taste, rich nutrition, no small thorns among muscles and the like, and is popular with farmers and consumers due to quick growth and delicious meat. In recent years, the market demand of the basha is continuously increased, the artificial breeding of the basha is gradually increased, but the basha often has the situation of heat stress resistance due to overhigh temperature in summer, so that the survival rate and the meat quality of the basha are influenced. CN103549208A discloses a compound premix feed for enhancing anti-stress ability of freshwater fish, which is not suitable for breeding of basha fish, and cannot improve anti-heat stress ability of basha fish. In order to adapt the basha to the temperature climate change, the stress reaction of the basha caused by the high-temperature floating of the tropical zone of the basha adapting to the tropical monsoon climate needs to be solved, and the quality of the basha can be effectively guaranteed.
Disclosure of Invention
The invention provides a culture method for improving heat stress resistance of basha, which effectively improves the heat stress resistance of the basha, adapts to climate change and improves the quality of the basha.
The technical scheme of the invention is realized as follows:
a breeding method for improving heat stress resistance of the Bass sand comprises the following steps: feeding the Chinese herbal additive in a Bass-Shake fish culture period, wherein the daily feeding amount is 1.5-3.0% of the weight of the fish, and stopping feeding for 7-10 days after continuously feeding for 2-4 days; feeding the traditional Chinese medicine additive again, wherein the daily feeding amount is 1.8-2.5% of the weight of the fish, stopping feeding after 2-4 days of continuous feeding, and naturally feeding; the traditional Chinese medicine additive is prepared from the following raw materials in parts by weight: 50-80 parts of hawthorn, 100 parts of dried orange peel, 150 parts of isatis root, 70-100 parts of rhizoma atractylodis and 50-80 parts of gentian.
Preferably, the traditional Chinese medicine additive is fed during the culture period, the daily feeding amount is 2.2 percent of the weight of the fish, and the feeding is stopped for 8 days after the continuous feeding for 3 days; feeding the traditional Chinese medicine additive again, wherein the daily feeding amount is 1.9% of the weight of the fish, stopping feeding after continuously feeding for 3 days, and naturally feeding. The traditional Chinese medicine additive is prepared from the following raw materials in parts by weight: 65 parts of hawthorn, 120 parts of dried orange peel, 85 parts of isatis root, 90 parts of rhizoma atractylodis and 70 parts of gentian.
Further, the preparation method of the traditional Chinese medicine additive comprises the following steps: the raw materials are crushed according to the weight parts, sieved by a sieve of 80-120 meshes, stirred for 25-30 min at 30-40 ℃ and 1000-1200 r/min, and granulated to obtain the finished product. Preferably, the raw materials are crushed, sieved by a 100-mesh sieve, stirred for 27min at the temperature of 38 ℃ and at the speed of 1050r/min, and granulated to obtain the finished product.
Further, the number of daily feeding is 2.
Compared with the prior art, the invention has the beneficial effects that:
the method for breeding the basha fish effectively improves the heat stress resistance of the basha fish, enables the basha fish to adapt to climate change better, effectively improves the survival rate of the basha fish, also effectively improves the growth speed of the basha fish, ensures the quality of the basha fish more effectively and improves the economic benefit. The traditional Chinese medicine additive with specific medicinal materials in a certain proportion effectively improves the heat stress resistance of the basha fish, promotes growth, improves survival rate and daily gain. By adopting the feeding mode of the invention, the heat stress resistance of the basha fish is further promoted, and the growth speed is improved. In addition, the traditional Chinese medicine additive is simple to prepare, is safer than other medicines with heat stress resistance additives, and is convenient to apply.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
Example 1
(1) Preparation of Chinese medicine additive
Taking the traditional Chinese medicine raw materials according to the following weight: 65 parts of hawthorn, 120 parts of dried orange peel, 85 parts of isatis root, 90 parts of rhizoma atractylodis and 70 parts of gentian, and crushing and sieving the materials with a 100-mesh sieve; stirring the above raw materials at 38 deg.C and 1050r/min for 27min, and granulating with a feed granulator to obtain granular Chinese medicinal feed as Chinese medicinal additive I.
(2) Bass-sand fish culture
Feeding the traditional Chinese medicine additive I during the breeding period of the basha fish, wherein the daily feeding amount is 2.2 percent of the weight of the fish, the feeding amount is divided into 2 times per day, and the feeding is stopped for 8 days after 3 days of continuous feeding; and feeding the traditional Chinese medicine additive I again, wherein the daily feeding amount is 1.9 percent of the weight of the fish, the daily feeding amount is divided into 2 times, and the feeding is stopped after 3 days of continuous feeding for natural feeding.
Example 2
(1) Preparation of Chinese medicine additive
Taking the traditional Chinese medicine raw materials according to the following weight: 50 parts of hawthorn, 150 parts of dried orange peel, 70 parts of isatis root, 100 parts of rhizoma atractylodis and 50 parts of gentian, and crushing and sieving the materials by a 100-mesh sieve; stirring the above raw materials at 38 deg.C and 1050r/min for 27min, and granulating with a feed granulator to obtain granular Chinese medicinal feed as Chinese medicinal additive II.
(2) Bass-sand fish culture
Feeding the traditional Chinese medicine additive II in a Bass-Sasa breeding period, wherein the daily feeding amount is 2.2% of the weight of the Bass-Sasa, feeding is divided into 2 times every day, and feeding is stopped for 8 days after 3 days of continuous feeding; and feeding the traditional Chinese medicine additive II again, wherein the daily feeding amount is 1.9 percent of the weight of the fish, the daily feeding amount is divided into 2 times, and the feeding is stopped after 3 days of continuous feeding for natural feeding.
Example 3
(1) Preparation of Chinese medicine additive
Taking the traditional Chinese medicine raw materials according to the following weight: 80 parts of hawthorn, 100 parts of dried orange peel, 100 parts of radix isatidis, 70-parts of rhizoma atractylodis and 80 parts of gentian, and crushing and sieving the materials by a 100-mesh sieve; stirring the above raw materials at 38 deg.C and 1050r/min for 27min, and granulating with a feed granulator to obtain granular Chinese medicinal feed as Chinese medicinal additive III.
(2) Bass-sand fish culture
Feeding the traditional Chinese medicine additive III during the culture period of the basha fish, wherein the daily feeding amount is 2.2 percent of the weight of the fish, the feeding amount is divided into 2 times every day, and the feeding is stopped for 8 days after 3 days of continuous feeding; and feeding the traditional Chinese medicine additive III again, wherein the daily feeding amount is 1.9 percent of the weight of the fish, the daily feeding amount is divided into 2 times, and the feeding is stopped after 3 days of continuous feeding for natural feeding.
Example 4
(1) Preparation of Chinese medicine additive
The Chinese medicinal additive I prepared in example 1 was used.
(2) Bass-sand fish culture
Feeding the traditional Chinese medicine additive I during the breeding period of the basha fish, wherein the daily feeding amount is 1.5 percent of the weight of the fish, the feeding amount is divided into 2 times every day, and the feeding is stopped for 7 days after 4 days of continuous feeding; and feeding the traditional Chinese medicine additive I again, wherein the daily feeding amount is 2.5 percent of the weight of the fish, the daily feeding amount is divided into 2 times, and the feeding is stopped after 2 days of continuous feeding for natural feeding.
Example 5
(1) Preparation of Chinese medicine additive
The Chinese medicinal additive I prepared in example 1 was used.
(2) Bass-sand fish culture
Feeding the traditional Chinese medicine additive I during the breeding period of the basha fish, wherein the daily feeding amount is 3.0 percent of the weight of the fish, the feeding amount is divided into 2 times per day, and the feeding is stopped for 10 days after 2 days of continuous feeding; and feeding the traditional Chinese medicine additive I again, wherein the daily feeding amount is 1.8 percent of the weight of the fish, the daily feeding amount is divided into 2 times, and the feeding is stopped after 4 days of continuous feeding for natural feeding.
Example 6
The main difference from the embodiment 1 lies in the preparation method of the traditional Chinese medicine additive, which is characterized in that the traditional Chinese medicine raw materials are taken according to the following weight: 65 parts of hawthorn, 120 parts of dried orange peel, 85 parts of isatis root, 90 parts of rhizoma atractylodis and 70 parts of gentian, and crushing and sieving the materials with a 100-mesh sieve; stirring the above raw materials at 30 deg.C and 1000r/min for 30min, and granulating with a feed granulator to obtain granular Chinese medicinal feed as Chinese medicinal additive I-1.
Example 7
The main difference from the embodiment 1 lies in the preparation method of the traditional Chinese medicine additive, which is characterized in that the traditional Chinese medicine raw materials are taken according to the following weight: 65 parts of hawthorn, 120 parts of dried orange peel, 85 parts of isatis root, 90 parts of rhizoma atractylodis and 70 parts of gentian, and crushing and sieving the materials with a 100-mesh sieve; stirring the above raw materials at 40 deg.C and 1200r/min for 25min, and granulating with a feed granulator to obtain granular Chinese medicinal feed as Chinese medicinal additive I-2.
Comparative example 1
The main difference between the comparative example and the example 1 is that the traditional Chinese medicine additive is prepared by replacing the malt with hawthorn and the medicated leaven with dried orange peel.
Comparative example 2
The main difference between the comparative example and the example 1 lies in the preparation of the traditional Chinese medicine additive, wherein the same amount of honeysuckle is replaced by isatis root, the same amount of dandelion is replaced by atractylodes rhizome, and the same amount of wrinkled gianthyssop herb is replaced by gentian.
Comparative example 3
This comparative example differs from example 1 in that: the traditional Chinese medicine additive comprises the following raw materials in parts by weight: 100 parts of hawthorn, 100 parts of dried orange peel, 100 parts of radix isatidis, 100 parts of rhizoma atractylodis and 100 parts of gentian.
Comparative example 4
The main difference between this comparative example and example 1 is that the barnacle culture process:
feeding the traditional Chinese medicine additive I during the breeding period of the basha fish, wherein the daily feeding amount is 1% of the weight of the fish, the feeding amount is divided into 2 times per day, and the feeding is stopped for 8 days after 3 days of continuous feeding; and feeding the traditional Chinese medicine additive I again, wherein the daily feeding amount is 1.5 percent of the weight of the fish, the daily feeding amount is divided into 2 times, and the feeding is stopped after 3 days of continuous feeding for natural feeding.
Test examples
Experiments were conducted in 7 months in 2020, outside the methylene blue compartment cultivation base in Haikou, Hainan province, and examples 1-7 were respectively set upGroups, comparative examples 1-4 and a control group (conventional culture group, i.e. no traditional Chinese medicine additive of the invention is fed), the groups of examples 1-7 and comparative examples 1-4 are cultured by the methods of examples 1-6 and comparative examples 1-4 on the basis of the conventional culture of the control group, and the culture scale of each test group is 100m2. Before the experiment begins, the size of the Pacific saury is 300-320 g, the stocking density is 10000 tails, the experiment period is 60 days, the indexes of daily average weight gain, survival rate and the like are counted, liver tissues are collected, and the anti-oxidation index of the liver is detected. The results are shown in tables 1 and 2 below.
TABLE 1 average daily gain and survival
Figure BDA0003235692970000051
Figure BDA0003235692970000061
TABLE 2 anti-oxidation index of liver
Figure BDA0003235692970000062
From the results, the method disclosed by the embodiment 1-7 of the invention has the advantages of high survival rate, great daily increase and high activity of liver superoxide dismutase.
Wherein, compared with the comparative examples 1-3, the traditional Chinese medicine additive with a certain proportion and specific medicinal materials is adopted to effectively improve the heat stress resistance of the basha fish and promote the growth of the basha fish. Example 1 in comparison with comparative example 4, the feeding method of the present invention further promotes the heat stress resistance of the basha fish and increases the growth rate.
In addition, the method of the embodiment 1 is applied to crucian carps, groupers and the like in 7 months in 2020, compared with the conventional culture, the daily gain is increased by less than 4%, the survival rate is increased by less than 6%, the activity of liver superoxide dismutase is increased by less than 5%, and the comprehensive evaluation shows that the method of the invention obviously reduces the heat stress resistance of other fishes. Therefore, the method disclosed by the invention has specificity on the basha fish.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (6)

1. A culture method for improving heat stress resistance of the Bass sand fish is characterized by comprising the following steps: the method comprises the following steps: feeding the Chinese herbal additive in a Bass-Shake fish culture period, wherein the daily feeding amount is 1.5-3.0% of the weight of the fish, and stopping feeding for 7-10 days after continuously feeding for 2-4 days; feeding the traditional Chinese medicine additive again, wherein the daily feeding amount is 1.8-2.5% of the weight of the fish, stopping feeding after 2-4 days of continuous feeding, and naturally feeding; the traditional Chinese medicine additive is prepared from the following raw materials in parts by weight: 50-80 parts of hawthorn, 100 parts of dried orange peel, 150 parts of isatis root, 70-100 parts of rhizoma atractylodis and 50-80 parts of gentian.
2. The culture method for improving the heat stress resistance of the basha fish according to claim 1, wherein the culture method comprises the following steps: the preparation method of the traditional Chinese medicine additive comprises the following steps: the raw materials are crushed according to the weight parts, sieved by a sieve of 80-120 meshes, stirred for 25-30 min at 30-40 ℃ and 1000-1200 r/min, and granulated to obtain the finished product.
3. The culture method for improving the heat stress resistance of the basha fish according to claim 1, wherein the culture method comprises the following steps: the method comprises the following steps: feeding traditional Chinese medicine additives in a culture period, wherein the daily feeding amount is 2.2% of the weight of the fish, and stopping feeding for 8 days after continuously feeding for 3 days; feeding the traditional Chinese medicine additive again, wherein the daily feeding amount is 1.9% of the weight of the fish, stopping feeding after continuously feeding for 3 days, and naturally feeding.
4. The culture method for improving heat stress resistance of the Bass sand fish according to claim 3, wherein the culture method comprises the following steps: the traditional Chinese medicine additive is prepared from the following raw materials in parts by weight: 65 parts of hawthorn, 120 parts of dried orange peel, 85 parts of isatis root, 90 parts of rhizoma atractylodis and 70 parts of gentian.
5. The culture method for improving heat stress resistance of the Bass sand fish according to claim 4, wherein the culture method comprises the following steps: pulverizing the raw materials, sieving with 100 mesh sieve, stirring at 38 deg.C and 1050r/min for 27min, and granulating to obtain the final product.
6. The culture method for improving heat stress resistance of the Bass sand fish according to any one of claims 1 to 5, wherein: the number of daily feeding is 2.
CN202111001180.1A 2021-05-14 2021-08-30 Breeding method for improving heat stress resistance of Bass sand fish Pending CN113796339A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170140601A (en) * 2016-06-13 2017-12-21 건국대학교 산학협력단 A feed additive comprising agricultural, forestry and marine products extract
CN110800653A (en) * 2019-11-29 2020-02-18 海南晨海水产有限公司 Heat stress resisting method for grouper culture
CN112638168A (en) * 2018-07-25 2021-04-09 联邦科学和工业研究组织 Method for feeding fish

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170140601A (en) * 2016-06-13 2017-12-21 건국대학교 산학협력단 A feed additive comprising agricultural, forestry and marine products extract
CN112638168A (en) * 2018-07-25 2021-04-09 联邦科学和工业研究组织 Method for feeding fish
CN110800653A (en) * 2019-11-29 2020-02-18 海南晨海水产有限公司 Heat stress resisting method for grouper culture

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
王晓力,周学辉主编: "《现代畜牧业高效养殖技术》", 31 July 2016 *

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