CN110754587A - Multifunctional compound feed for oplegnathus punctatus and preparation method thereof - Google Patents

Multifunctional compound feed for oplegnathus punctatus and preparation method thereof Download PDF

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CN110754587A
CN110754587A CN201911254876.8A CN201911254876A CN110754587A CN 110754587 A CN110754587 A CN 110754587A CN 201911254876 A CN201911254876 A CN 201911254876A CN 110754587 A CN110754587 A CN 110754587A
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parts
mixture
compound feed
oplegnathus
preparation
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李伟
蔡有森
蔡春有
蔡建顺
黄春仁
蔡四川
蔡金泉
蔡惠明
张国庆
蔡智怀
陈猛猛
吴光灿
陈艺斌
刘赐福
李伟峰
蔡艺新
王景宝
罗磊
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Hainan Chenhai Aquatic Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
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    • A23K20/10Organic substances
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/26Compounds containing phosphorus
    • AHUMAN NECESSITIES
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    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/10Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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Abstract

The invention provides a multifunctional compound feed for oplegnathus punctatus and a preparation method thereof, wherein the multifunctional compound feed comprises the following components: 25-30 parts of fish meal, 10-15 parts of broad bean powder, 10-20 parts of dextrin, 10-15 parts of cellulose, 2-3 parts of fish oil, 0.5-1 part of soybean lecithin, 1-2 parts of mineral mixture, 0.3-0.5 part of vitamin mixture, 0.1-0.2 part of choline chloride, 0.1-0.5 part of monocalcium phosphate, 0.01-0.05 part of glycerol butyrate mixture and 0.01-0.03 composite enzyme preparation; a glycerol butyrate mixture comprising: 1-2 parts of glycerol monobutyrate, 1-3 parts of glycerol dibutyrate, 3-5 parts of glycerol tributyrate, 1-2 parts of vitamin C-phosphate and 1-3 parts of mannan oligosaccharide. The multifunctional feed can enhance the immunoregulation of fish intestinal tract, increase growth speed, improve growth performance, make fish muscle texture more compact, and has good elasticity and chewiness.

Description

Multifunctional compound feed for oplegnathus punctatus and preparation method thereof
Technical Field
The invention relates to the technical field of aquaculture feed, and particularly relates to a porgy multifunctional compound feed and a preparation method thereof.
Background
The Oplegnathus punctatus (Oplegnathuspurtus) belongs to the genus Oplegnathus of family Paralithoidae of order Perciformes, and is commonly called Pagrus punctatus, black mouth, Oplegnathus fasciatus, etc. The body of the oplegnathus punctatus is dark brown, has irregular black spots, short small head and mouth, dense and sharp teeth, short and hard dorsal fin, obviously raised front fin ray, short back part and truncated shape at the rear edge of the fin strip part. Widely distributed in the island of Taiwan in Korea, Japan and China, and in the sea areas of south China, east China, yellow sea, etc., and belongs to tropical fishes. The oplegnathus punctatus has high edible and medicinal values, fine and smooth meat quality, rich collagen, unique taste and rich various amino acids and unsaturated fatty acids.
At present, the artificial breeding of the oplegnathus fasciatus is relatively less, and the existing breeding of the oplegnathus fasciatus mainly adopts two modes of cage breeding and pond breeding. However, in the process of artificial breeding, the food composition of the fish is often changed, so that the life style, the exercise capacity, the feeding capacity, the immunity and the like are changed, and the artificially bred oplegnathus maculatus has soft muscle texture, light flavor and low chewiness compared with wild fish, and the taste and the quality of the oplegnathus maculatus are greatly influenced. Therefore, the nutrition of the feed for the oplegnathus punctatus needs to be optimized and controlled, the growth quality of the fish is improved, the muscle nutrient content and the taste quality of the oplegnathus punctatus are closer to those of the wild oplegnathus punctatus, and the application prospect of the artificial culture technology of the oplegnathus punctatus is further improved.
Disclosure of Invention
In view of the above, the invention provides a multifunctional compound feed for oplegnathus punctatus and a preparation method thereof.
The technical scheme of the invention is realized as follows:
the invention provides a multifunctional compound feed for oplegnathus punctatus, which comprises the following raw material components in parts by weight: 25-30 parts of fish meal, 10-15 parts of broad bean powder, 10-20 parts of dextrin, 10-15 parts of cellulose, 2-3 parts of fish oil, 0.5-1 part of soybean lecithin, 1-2 parts of mineral mixture, 0.3-0.5 part of vitamin mixture, 0.1-0.2 part of choline chloride, 0.1-0.5 part of monocalcium phosphate, 0.01-0.05 part of glycerol butyrate mixture and 0.01-0.03 composite enzyme preparation; the butyrin mixture comprises the following components in parts by weight: 1-2 parts of glycerol monobutyrate, 1-3 parts of glycerol dibutyrate, 3-5 parts of glycerol tributyrate, 1-2 parts of vitamin C-phosphate and 1-3 parts of mannan oligosaccharide. The multifunctional feed can effectively promote the digestion and absorption of nutrient substances, improve the immunoregulation of fish intestinal tracts, improve the growth speed of the oplegnathus fasciatus and improve the growth performance of the oplegnathus fasciatus, so that the fish muscle texture is more compact, elastic, good in chewiness and delicious in flavor, and the muscle nutrient components and the taste quality of the artificially cultured oplegnathus fasciatus are closer to those of the wild oplegnathus fasciatus.
Further explaining, the beverage comprises the following raw material components in parts by weight: 28 parts of fish meal, 13 parts of broad bean powder, 15 parts of dextrin, 12 parts of cellulose, 2.5 parts of fish oil, 0.8 part of soybean lecithin, 1.5 parts of mineral mixture, 0.4 part of vitamin mixture, 0.15 part of choline chloride, 0.3 part of monocalcium phosphate, 0.03 part of butyrin mixture and 0.02 part of complex enzyme preparation.
Further, the glyceryl butyrate mixture comprises the following components in parts by weight: 1.5 parts of glycerol monobutyrate, 2 parts of glycerol dibutyrate, 4 parts of glycerol tributyrate, 1.5 parts of vitamin C-phosphate and 2 parts of mannan oligosaccharide.
Further, the complex enzyme preparation comprises 10000-30000 IU/g of xylanase, 5000-8000 IU/g of mannase, 1000-3000 IU/g of β -glucanase, 1000-3000 IU/g of pectinase, 1000-2000 IU/g of cellulase and 5000-10000 IU/g of phytase.
The composite microecological preparation is prepared by mixing a bacillus coagulans microbial agent, a bacillus natto microbial agent, a saccharomyces cerevisiae microbial agent and an aspergillus niger microbial agent according to the weight ratio of 1:3:3: 2. By adding the compound microecological preparation, the digestion and absorption of fish intestinal tracts are effectively promoted, the viscosity of digestive minced esophagus is reduced, beneficial microorganisms in a water body are adjusted, and the disease resistance and the growth speed of the oplegnathus punctatus are improved.
Further, the cellulose is a composite vitamin formed by mixing carboxymethyl cellulose and microcrystalline cellulose according to a mass ratio of 1 (2-3). The loss rate of the feed in the water body is reduced through the composite cellulose in a certain proportion, and the full digestion and absorption of fish intestinal tracts are ensured.
A preparation method of a multifunctional compound feed for oplegnathus punctatus comprises the following steps:
(1) weighing fish meal, broad bean powder, dextrin, cellulose, choline chloride and monocalcium phosphate according to a certain proportion, crushing, sieving by a sieve of 60-100 meshes, and uniformly mixing and stirring to obtain a primary mixture;
(2) adding a mineral mixture, a vitamin mixture, a butyrin mixture and a complex enzyme preparation into the mixed solid powder, and uniformly mixing at 25-28 ℃ to obtain a secondary mixture;
(3) adding the fish oil and the soybean lecithin into the secondary mixture, uniformly stirring and mixing at the speed of 100-120 r/min, and granulating at the low temperature of 45-55 ℃ by using a granulator to obtain granulated feed with the particle size of 5-8 mm;
(4) and (3) drying the red sea bream by hot air at a low temperature of 30-35 ℃ for 15-20 h until the water content is 5-10%, thus obtaining the multifunctional compound feed for the oplegnathus punctatus. According to the invention, the raw materials are mixed for multiple times at different temperatures, and meanwhile, the raw materials are subjected to low-temperature granulation and slow low-temperature hot air drying, so that the components in the feed are favorably kept, the homogeneity of the feed is improved, other additives are not required to be added, and the digestion and absorption of the oplegnathus maculatus are favorably realized.
Further explaining, in the step (2), the composite microecological preparation is added into the secondary mixture, and after the mixture is uniformly mixed and stirred, the mixture is preserved for 20-30 min at a constant temperature of 40-45 ℃.
Further explaining, in the step (3), adding the fish oil and the soybean lecithin into the secondary mixture, uniformly stirring and mixing at 110-115 r/min, and granulating at a low temperature of 50 ℃ by using a granulator to obtain the granulated feed with the particle size of 5-8 mm.
Further explaining, in the step (4), the porgy multi-functional compound feed is obtained by drying the porgy multi-functional compound feed for 18 hours at the low temperature of 32 ℃ until the water content is 8 percent.
Compared with the prior art, the invention has the beneficial effects that: the multifunctional compound feed for the oplegnathus punctatus adopts fish meal and broad bean powder as protein sources, optimizes the feed components, takes soybean lecithin and fish oil as fat sources, and is added with a glycerol butyrate mixture and a compound enzyme preparation to combine the soybean lecithin and the fish oil with a mixed protein source and a mixed fat source, so that the digestion and absorption of nutrient substances are effectively promoted, the growth speed of the oplegnathus punctatus is increased, the ingestion capability and the anti-stress capability of the oplegnathus punctatus are enhanced, the immunoregulation of the fish intestinal tract is enhanced, the movement capability of fish is improved, the growth performance of the oplegnathus punctatus is improved, the muscle texture of the fish is more compact, the fish is rich in elasticity, the chewiness is good, and the flavor is delicious, so that the muscle nutrient components and the taste quality of the artificially cultured oplegnatured oplegnathus punctatus are closer to those of the wild oplegnatured.
And the compound microecological preparation is added, so that the digestion and absorption of fish intestinal tracts are effectively promoted, the viscosity of digestive minced esophagus is reduced, beneficial microorganisms in a water body are regulated, and the disease resistance and the growth performance of the oplegnathus fasciatus are further improved.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
Embodiment 1-a multifunctional compound feed for oplegnathus punctatus comprises the following raw material components in parts by weight:
25 parts of fish meal, 15 parts of broad bean powder, 10 parts of dextrin, 10 parts of carboxymethyl cellulose, 2 parts of fish oil, 1 part of soybean lecithin, 1 part of mineral mixture, 0.3 part of vitamin mixture, 0.1 part of choline chloride, 0.1 part of monocalcium phosphate, 0.05 part of butyrin mixture and 0.03 part of complex enzyme preparation;
the butyrin mixture comprises the following components in parts by weight: 1 part of glycerol monobutyrate, 3 parts of glycerol dibutyrate, 3 parts of glycerol tributyrate, 2 parts of vitamin C-phosphate and 1 part of mannan oligosaccharide.
The compound enzyme preparation is aquatic compound enzyme MTS-003, the mineral mixture is compound seawater fish microelement premix C1420, and the vitamin mixture is compound vitamin SFV1, which are conventional commercial products.
The preparation method of the multifunctional compound feed for the oplegnathus punctatus comprises the steps of weighing the raw materials in proportion, sequentially adding the raw materials into the raw materials, uniformly mixing the raw materials, granulating the mixture by a granulator to obtain a granulated feed, and finally drying the granulated feed to obtain the multifunctional compound feed for the oplegnathus punctatus.
Embodiment 2-a multifunctional compound feed for oplegnathus punctatus comprises the following raw material components in parts by weight:
30 parts of fish meal, 10 parts of broad bean powder, 20 parts of dextrin, 15 parts of carboxymethyl cellulose, 3 parts of fish oil, 0.5 part of soybean lecithin, 2 parts of mineral mixture, 0.5 part of vitamin mixture, 0.2 part of choline chloride, 0.5 part of monocalcium phosphate, 0.01 part of glycerol butyrate mixture, 0.01 part of complex enzyme preparation and 0.01 part of complex microecological preparation;
the butyrin mixture comprises the following components in parts by weight: 2 parts of glycerol monobutyrate, 1 part of glycerol dibutyrate, 5 parts of glycerol tributyrate, 1 part of vitamin C-phosphate and 3 parts of mannan oligosaccharide.
The compound enzyme preparation is aquatic compound enzyme MTS-003, the mineral mixture is compound seawater fish microelement premix C1420, and the vitamin mixture is compound vitamin SFV1, which are conventional commercial products.
The composite microecological preparation is prepared by mixing a bacillus coagulans microbial agent, a bacillus natto microbial agent, a saccharomyces cerevisiae microbial agent and an aspergillus niger microbial agent according to the weight ratio of 1:3:3: 2.
The preparation method of the multifunctional compound feed for the oplegnathus punctatus comprises the steps of weighing the raw materials in proportion, sequentially adding the raw materials into the raw materials, uniformly mixing the raw materials, granulating the mixture by a granulator to obtain a granulated feed, and finally drying the granulated feed to obtain the multifunctional compound feed for the oplegnathus punctatus.
Embodiment 3-a multifunctional compound feed for oplegnathus punctatus comprises the following raw material components in parts by weight:
28 parts of fish meal, 13 parts of broad bean powder, 15 parts of dextrin, 12 parts of cellulose, 2.5 parts of fish oil, 0.8 part of soybean lecithin, 1.5 parts of mineral mixture, 0.4 part of vitamin mixture, 0.15 part of choline chloride, 0.3 part of monocalcium phosphate, 0.03 part of glyceryl butyrate mixture, 0.02 part of complex enzyme preparation and 0.02 part of complex microecological preparation;
the butyrin mixture comprises the following components in parts by weight: 1.5 parts of glycerol monobutyrate, 2 parts of glycerol dibutyrate, 4 parts of glycerol tributyrate, 1.5 parts of vitamin C-phosphate and 2 parts of mannan oligosaccharide.
The compound enzyme preparation is aquatic compound enzyme MTS-003, the mineral mixture is compound seawater fish microelement premix C1420, the vitamin mixture is compound vitamin SFV1 which are conventional commercial products, and the cellulose is compound vitamin formed by mixing carboxymethyl cellulose and microcrystalline cellulose according to the mass ratio of 1: 2.
The composite microecological preparation is prepared by mixing a bacillus coagulans microbial agent, a bacillus natto microbial agent, a saccharomyces cerevisiae microbial agent and an aspergillus niger microbial agent according to the weight ratio of 1:3:3: 2.
The preparation method of the multifunctional compound feed for the oplegnathus punctatus comprises the steps of weighing the raw materials in proportion, sequentially adding the raw materials into the raw materials, uniformly mixing the raw materials, granulating the mixture by a granulator to obtain a granulated feed, and finally drying the granulated feed to obtain the multifunctional compound feed for the oplegnathus punctatus.
Embodiment 4-a multifunctional compound feed for oplegnathus punctatus comprises the following raw material components in parts by weight:
28 parts of fish meal, 13 parts of broad bean powder, 15 parts of dextrin, 12 parts of cellulose, 2.5 parts of fish oil, 0.8 part of soybean lecithin, 1.5 parts of mineral mixture, 0.4 part of vitamin mixture, 0.15 part of choline chloride, 0.3 part of monocalcium phosphate, 0.03 part of glycerol butyrate mixture, 0.02 part of complex enzyme preparation and 0.02 part of complex microecological preparation;
the butyrin mixture comprises the following components in parts by weight: 1.5 parts of glycerol monobutyrate, 2 parts of glycerol dibutyrate, 4 parts of glycerol tributyrate, 1.5 parts of vitamin C-phosphate and 2 parts of mannan oligosaccharide.
The complex enzyme preparation comprises 20000 IU/g of xylanase, 6500 IU/g of mannase, 2000 IU/g of β -glucanase, 2000 IU/g of pectinase, 2000 IU/g of cellulase and 8000 IU/g of phytase.
The mineral mixture comprises the following components in percentage by weight: 40 parts of magnesium sulfate, 50 parts of sodium dihydrogen phosphate, 33 parts of potassium dihydrogen phosphate, 8 parts of sodium chloride, 15 parts of ferrous sulfate, 5 parts of calcium lactate, 9 parts of starch, 0.7 part of zinc sulfate, 0.5 part of manganese sulfate, 0.05 part of copper sulfate, 0.02 part of cobalt sulfate and 0.01 part of potassium iodide.
The vitamin mixture comprises the following components in percentage by weight: 95 parts of starch, 0.8 part of vitamin B1, 0.8 part of vitamin B2, 0.8 part of vitamin B6, 6 parts of vitamin C, 8 parts of vitamin A, 0.8 part of vitamin D, 2 parts of vitamin E, 0.3 part of vitamin K, 3 parts of pantothenic acid, 0.08 part of folic acid, 6 parts of inositol, 6 parts of nicotinic acid and 0.002 part of biotin.
The cellulose is a composite vitamin formed by mixing carboxymethyl cellulose and microcrystalline cellulose according to the mass ratio of 1: 3.
The composite microecological preparation is prepared by mixing a bacillus coagulans microbial agent, a bacillus natto microbial agent, a saccharomyces cerevisiae microbial agent and an aspergillus niger microbial agent according to the weight ratio of 1:3:3: 2.
The preparation method of the multifunctional compound feed for the oplegnathus punctatus comprises the steps of weighing the raw materials in proportion, sequentially adding the raw materials into the raw materials, uniformly mixing the raw materials, granulating the mixture by a granulator to obtain a granulated feed, and finally drying the granulated feed to obtain the multifunctional compound feed for the oplegnathus punctatus.
Embodiment 5-a method for preparing a multifunctional compound feed for oplegnathus punctatus, comprising the following steps:
(1) according to the formula components of the multifunctional compound feed for the oplegnathus punctatus in the embodiment 4, the fish meal, the broad bean powder, the dextrin, the cellulose, the choline chloride and the monocalcium phosphate are weighed according to the proportion, crushed, sieved by a sieve of 60-100 meshes, and uniformly mixed and stirred to obtain a primary mixture;
(2) adding a mineral mixture, a vitamin mixture, a butyrin mixture and a complex enzyme preparation into the mixed solid powder, uniformly mixing at 25-28 ℃ to obtain a secondary mixture, adding a complex microecological preparation, uniformly mixing and stirring, and preserving at a constant temperature of 40-45 ℃ for 20-30 min;
(3) adding the fish oil and the soybean lecithin into the secondary mixture, uniformly stirring and mixing at the speed of 100-120 r/min, and granulating at the low temperature of 45-55 ℃ by using a granulator to obtain granulated feed with the particle size of 5-8 mm;
(4) and (3) drying the red sea bream by hot air at a low temperature of 30-35 ℃ for 15-20 h until the water content is 5-10%, thus obtaining the multifunctional compound feed for the oplegnathus punctatus.
Comparative example 1-method for preparing a multifunctional compound feed for oplegnathus punctatus according to example 4, the difference is that: the same as in example 4 was repeated except that 0.03 parts of the butyrin mixture was replaced with 0.03 parts of tributyrin.
Comparative example 2-method for preparing a multifunctional compound feed for oplegnathus punctatus according to example 4, the difference is that: the butyrin mixture comprises the following components in parts by weight: 1.5 parts of glycerol monobutyrate, 2 parts of glycerol dibutyrate, 4 parts of glycerol tributyrate, 1.5 parts of vitamin C-phosphate, and the rest are the same as in example 4.
Comparative example 3-method for preparing a multifunctional compound feed for oplegnathus punctatus according to example 4, the difference is that: the components and the proportion of the complex enzyme preparation are removed, and the rest is the same as the embodiment 4.
Comparative example 4-method for preparing a multifunctional compound feed for oplegnathus punctatus according to example 4, the difference is that: 28 parts of fish meal and 13 parts of broad bean powder were replaced with 41 parts of fish meal, and the rest was the same as in example 4.
The multifunctional compound feed for the oplegnathus fasciatus, prepared in the examples 1-5 and the comparative examples 1-4, is used for feeding the oplegnathus fasciatus in a pond farm, wherein the pond farm is divided into 9 independent culture areas, the independent culture areas are used as 9 experimental groups, 50 oplegnathus fasciatus are fed in each pond, the oplegnathus fasciatus is fed for 2 times every day, the oplegnathus fasciatus is fed according to the weight of 1% -3%, and after the oplegnathus fasciatus is fed for 2 months, the pond culture effect of the oplegnathus fasciatus is counted, and the following table is shown:
1. before the experiment, measuring the weight of the fish in each experimental group, and taking an average value; after the experiment was completed and feeding was stopped for 1 day, the fish weight in each experimental group was measured and averaged.
2. And (5) measuring the conventional nutrient components. The method comprises the following steps: determination of crude protein content, crude fat content and total carbohydrate. Wherein, the crude protein content is determined by adopting a Kjeldahl method, the crude fat content is determined by adopting a Soxhlet extraction method, and the water content is determined by adopting a constant-temperature drying method under normal pressure. The total carbohydrate assay comprises total sugar and cellulose content measurements with the results as given in table 1 below.
3. And (4) measuring the texture. Taking the intact fish meat at the same part of the oplegnathus fasciatus sample of each experimental group, determining each texture parameter mainly comprising elasticity, chewiness and cohesiveness by a texture analyzer in a Texture Profile Analysis (TPA) mode, repeatedly determining each parameter for 3 times, and taking an average value, wherein the results are shown in the following table 2.
TABLE 1 comparison of conventional nutritional ingredient determination
As can be seen from the above table, in the embodiments 1 to 5 of the present invention, compared with the comparative examples 1 to 4, the growth rate and the nutrient content of the oplegnathus maculatus are significantly increased, and in the artificial breeding process, no phenomena such as head floating, swimming test and the like of the oplegnathus maculatus occur, and no inflammation and swelling of intestinal tracts occur after dissection.
Comparing examples 1 and 2, it is known that the compound microecological preparation can further effectively promote the digestion and absorption of fish intestinal tracts, reduce the viscosity of digestive esophageal paste, and regulate beneficial microorganisms in water, so that the disease resistance and growth speed of the oplegnathus punctatus are improved.
From the embodiment 2 and the embodiment 3, the loss rate of the feed in the water body is reduced by the composite cellulose in a certain proportion, the full digestion and absorption of the fish intestinal tract are ensured, and the accumulation of nutrient components is facilitated.
As can be seen from the comparison between the embodiment 4 and the comparative examples 1 to 3, the fish meal and the broad bean powder are used as protein sources, meanwhile, the feed components are optimized, the soybean lecithin and the fish oil are used as fat sources, and the butyrin mixture and the complex enzyme preparation are added, so that the feed components are combined with the mixed protein source and the mixed fat source, the digestion and absorption of fish intestinal tracts are effectively promoted, the immunoregulation is improved, the ingestion ability, the anti-stress ability and the immunity of the oplegnathus maculatus are enhanced, and the growth performance of the oplegnathus maculatus is effectively improved.
TABLE 2 comparison of muscle texture parameters
Figure BDA0002309938130000091
As can be seen from the above table, the elasticity, chewiness and cohesiveness of the muscle of the oplegnathus punctatus are significantly increased in examples 1 to 5 of the present invention as compared with comparative examples 1 to 4. The multifunctional feed for the oplegnathus punctatus, prepared by the invention, is shown to effectively improve the movement ability of fish, further improve the growth performance of the oplegnathus punctatus, and enable the muscle texture of the fish to be more compact, elastic, good in chewiness and delicious in flavor, so that the muscle nutrient content and the taste quality of the artificially cultured oplegnathus punctatus are closer to those of the wild oplegnathus punctatus.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (10)

1. A multifunctional compound feed for oplegnathus punctatus is characterized in that: the composite material comprises the following raw material components in parts by weight: 25-30 parts of fish meal, 10-15 parts of broad bean powder, 10-20 parts of dextrin, 10-15 parts of cellulose, 2-3 parts of fish oil, 0.5-1 part of soybean lecithin, 1-2 parts of mineral mixture, 0.3-0.5 part of vitamin mixture, 0.1-0.2 part of choline chloride, 0.1-0.5 part of monocalcium phosphate, 0.01-0.05 part of glycerol butyrate mixture and 0.01-0.03 composite enzyme preparation;
the butyrin mixture comprises the following components in parts by weight: 1-2 parts of glycerol monobutyrate, 1-3 parts of glycerol dibutyrate, 3-5 parts of glycerol tributyrate, 1-2 parts of vitamin C-phosphate and 1-3 parts of mannan oligosaccharide.
2. The multifunctional compound feed for oplegnathus punctatus according to claim 1, wherein the compound feed comprises the following components in parts by weight: the composite material comprises the following raw material components in parts by weight: 28 parts of fish meal, 13 parts of broad bean powder, 15 parts of dextrin, 12 parts of cellulose, 2.5 parts of fish oil, 0.8 part of soybean lecithin, 1.5 parts of mineral mixture, 0.4 part of vitamin mixture, 0.15 part of choline chloride, 0.3 part of monocalcium phosphate, 0.03 part of butyrin mixture and 0.02 part of complex enzyme preparation.
3. The multifunctional compound feed for oplegnathus punctatus according to claim 2, wherein the compound feed comprises the following components in parts by weight: the butyrin mixture comprises the following components in parts by weight: 1.5 parts of glycerol monobutyrate, 2 parts of glycerol dibutyrate, 4 parts of glycerol tributyrate, 1.5 parts of vitamin C-phosphate and 2 parts of mannan oligosaccharide.
4. The multifunctional compound feed for oplegnathus fasciatus as claimed in claim 3, wherein the compound enzyme preparation comprises 10000-30000 IU/g of xylanase, 5000-8000 IU/g of mannase, 1000-3000 IU/g of β -glucanase, 1000-3000 IU/g of pectinase, 1000-2000 IU/g of cellulase and 5000-10000 IU/g of phytase.
5. The multifunctional compound feed for oplegnathus punctatus according to claim 4, wherein the compound feed comprises the following components in parts by weight: the composite microecological preparation is prepared by mixing a bacillus coagulans microbial agent, a bacillus natto microbial agent, a saccharomyces cerevisiae microbial agent and an aspergillus niger microbial agent according to the weight ratio of 1:3:3: 2.
6. The multifunctional compound feed for oplegnathus punctatus according to claim 1, wherein the compound feed comprises the following components in parts by weight: the cellulose is a composite vitamin formed by mixing carboxymethyl cellulose and microcrystalline cellulose according to a mass ratio of 1 (2-3).
7. A preparation method of the multifunctional compound feed for oplegnathus punctatus as claimed in any one of claims 1 to 6, wherein the preparation method comprises the following steps: the method comprises the following steps:
(1) weighing fish meal, broad bean powder, dextrin, cellulose, choline chloride and monocalcium phosphate according to a certain proportion, crushing, sieving by a sieve of 60-100 meshes, and uniformly mixing and stirring to obtain a primary mixture;
(2) adding a mineral mixture, a vitamin mixture, a butyrin mixture and a complex enzyme preparation into the mixed solid powder, and uniformly mixing at 25-28 ℃ to obtain a secondary mixture;
(3) adding the fish oil and the soybean lecithin into the secondary mixture, uniformly stirring and mixing at the speed of 100-120 r/min, and granulating at the low temperature of 45-55 ℃ by using a granulator to obtain granulated feed with the particle size of 5-8 mm;
(4) and (3) drying the red sea bream by hot air at a low temperature of 30-35 ℃ for 15-20 h until the water content is 5-10%, thus obtaining the multifunctional compound feed for the oplegnathus punctatus.
8. The preparation method of the multifunctional compound feed for oplegnathus punctatus according to claim 7, wherein the preparation method comprises the following steps: and (2) adding the composite microecologics into the secondary mixture, uniformly mixing and stirring, and preserving at constant temperature of 40-45 ℃ for 20-30 min.
9. The preparation method of the multifunctional compound feed for oplegnathus punctatus according to claim 7, wherein the preparation method comprises the following steps: and (3) adding the fish oil and the soybean lecithin into the secondary mixture, uniformly stirring and mixing at 110-115 r/min, and granulating at a low temperature of 50 ℃ by using a granulator to obtain the granulated feed with the particle size of 5-8 mm.
10. The preparation method of the multifunctional compound feed for oplegnathus punctatus according to claim 7, wherein the preparation method comprises the following steps: and (4) drying the oplegnathus fasciatus by hot air at a low temperature of 32 ℃ for 18 hours until the water content is 8 percent, thus obtaining the multifunctional compound feed for the oplegnathus fasciatus.
CN201911254876.8A 2019-12-10 2019-12-10 Multifunctional compound feed for oplegnathus punctatus and preparation method thereof Pending CN110754587A (en)

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