CN110754358A - Device and method for adjusting fruit setting rate of fruit trees by position and flow double-control liquid pollination - Google Patents

Device and method for adjusting fruit setting rate of fruit trees by position and flow double-control liquid pollination Download PDF

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CN110754358A
CN110754358A CN201911290192.3A CN201911290192A CN110754358A CN 110754358 A CN110754358 A CN 110754358A CN 201911290192 A CN201911290192 A CN 201911290192A CN 110754358 A CN110754358 A CN 110754358A
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liquid
pollination
fruit
spray
protective cover
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CN110754358B (en
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沈向
杨露
王芸芸
张璐璐
毛云飞
殷昕羽
尹伊君
宿夏菲
刘业萍
庄会灵
胡艳丽
毛志泉
陈学森
王文莉
魏绍冲
陈晓流
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Shandong Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/027Apparatus for pollination

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Abstract

The invention discloses a liquid pollination spraying device and a method for carrying out liquid pollination by using the device. The device comprises: the spray rod is connected with the liquid storage barrel, and at least one spray head is arranged on the spray rod; the spray head is provided with a protective cover; one end of the protective cover is fixed on the spray rod, and the other end of the protective cover is provided with an opening; and a shutter cover is arranged at one open end of the protective cover. During pollination, the length of the spray rod and the number of the spray nozzles are determined according to the height of a fruit tree to be pollinated and the distribution position of flowers, the angle of fan blades on the louver is adjusted according to the load of the fruit tree, and the concentration of pollen in the liquid pollination liquid is adjusted according to the fruit setting rate required. The invention greatly reduces the labor intensity and improves the efficiency; can be flexibly adjusted according to the orchard load. The fruit setting agent is simple and practical, convenient to use, wide in application range and low in cost, and can greatly reduce labor for pollination and flower and fruit thinning in an orchard.

Description

Device and method for adjusting fruit setting rate of fruit trees by position and flow double-control liquid pollination
Technical Field
The invention relates to the technical field of fruit tree pollination, in particular to a device and a method for adjusting fruit setting rate of fruit trees by position-flow double-control liquid pollination.
Background
Plant self-incompatibility is a ubiquitous mechanism in angiosperms that limits self-fertilization, preventing pollen tubes of the same genotype from growing normally in pistils, fertilizing. The reaction involved more than 70 families, more than 250 genera. The occurrence of self-incompatibility prevents close-relative propagation, promotes cross pollination and fertilization, and is beneficial to species diversity. The types of self-incompatibility can be classified into sporophytes and gametophytes according to their control factors. Gametophytic self-incompatibility refers to the fact that in the interaction process of pollen and pistil, the behavior of the pollen is determined by the property of pollen grain inner wall protein, namely the haploid genotype of the pollen grain, and various fruit trees of the Rosaceae, such as pears, apples, sweet cherries, apricots, prunus mume, plums, almonds and the like, show gametophytic self-incompatibility. The phenomenon of self-incompatibility has pushed species evolution to some extent, but also poses certain obstacles in the production of commercial crops, such as apple production. Because the self-pollination of the fruit trees can not fruit, pollination varieties must be configured in production, and fruit setting can be ensured only by artificial supplementary pollination, thereby increasing the production cost. However, after pollination is completed, the fruit setting quantity is far beyond the production requirement, the fruit quality cannot be guaranteed, and flower thinning and fruit thinning can be performed again. Flower thinning and fruit thinning are necessary measures for improving fruit setting rate and fruit quality of fruit trees, and are important for high yield and stable yield of orchards and improvement of fruit quality. In the prior art, flower and fruit thinning operation completely depends on manual operation, a large amount of labor is needed, and as labor cost rises, manual flower and fruit thinning becomes a bottleneck problem restricting orchard benefit. Although the cost of chemical flower thinning and fruit thinning is low, the effect is extremely unstable, and the chemical flower thinning and fruit thinning cannot be popularized in a large area for many years. How to balance the contradiction between the pollination and the fruit setting, realize the double regulation and control of pollination and fruit setting and realize the simplified cultivation is the starting point of the invention.
Disclosure of Invention
Aiming at the prior art, the invention aims to provide a device for adjusting fruit setting rate of a fruit tree by position and flow double-control liquid pollination, which adjusts the concentration and the spray volume of the liquid pollination according to the expected yield and adjusts the pollination position according to the flower volume distribution, thereby adjusting and controlling the fruit setting rate of the fruit tree in the flowering phase.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a position and flow double-control liquid pollination spraying device, which comprises a liquid storage barrel and a spray rod connected with the liquid storage barrel, and is characterized in that at least one spray head is arranged on the spray rod; the spray head is provided with a protective cover; one end of the protective cover is fixed on the spray rod, and the other end of the protective cover is provided with an opening; and a shutter cover is arranged at one open end of the protective cover.
Preferably, a plurality of spray heads are arranged on the spray rod, and the distance between the spray heads is 30-50 cm.
Preferably, a plurality of fan blades are arranged on the louver cover; the fan blades are movably connected with a longitudinal fan blade shaft.
Preferably, the lower end of the fan blade shaft is movably connected with a sliding groove fixed on the outer edge of the protective cover, and a fastening bolt is arranged on the sliding groove.
Preferably, the bottommost part of the open end of the protective cover is connected with the solution backflow branch pipe.
Preferably, the solution backflow branch pipe is connected with the multi-way connector, and one end of the multi-way connector is connected with the liquid storage barrel through the solution backflow header pipe.
The second aspect of the invention provides the application of the position-flow double-control liquid pollination spraying device in adjusting the fruit setting rate of fruit trees.
The third aspect of the invention provides a method for adjusting fruit setting rate of a fruit tree by using a position-flow double-control liquid pollination spraying device, which comprises the following steps:
(1) building a garden for a single variety, or unifying pollinated trees to main cultivated varieties by a high grafting and head changing method, and uniformly shaping and pruning to ensure that the tree height, the crown diameter and the branch structure are consistent as much as possible;
(2) determining the length of a spray rod and the number of spray heads according to the height of a fruit tree to be pollinated and the distribution position of flowers, adjusting the angle of fan blades on a louver cover according to the distribution position of the flowers and the load of the fruit tree, and adjusting the concentration of pollen in liquid pollination liquid according to the fruit setting rate required to be achieved; spraying the liquid pollination liquid on fruit trees to be pollinated by using the position flow double-control liquid pollination spraying device of any one of claims 1 to 6.
Preferably, the liquid pollination liquid comprises the following raw materials in parts by weight: 4-10 parts of pollen, 4-6 parts of xanthomonas polysaccharide, 120-180 parts of cane sugar, 10-15 parts of borax and 10000 parts of water.
Preferably, the liquid pollination liquid is prepared by the following method:
A. adding weighed xanthomonas polysaccharide and sucrose into hot water at 70-80 ℃, continuously stirring until the xanthomonas polysaccharide and the sucrose are completely dissolved, adding borax and stirring;
B. and D, when the solution is cooled to room temperature, adding the pollen into the solution prepared in the step A, and continuously stirring until the pollen is uniformly mixed, thus completing the preparation.
The invention has the beneficial effects that:
the liquid pollination equipment can spray pollen liquid with different densities at different height positions of a fruit tree, greatly reduces the labor intensity and improves the efficiency. The design of shower nozzle position and quantity, the scope and the quantity that can more convenient change pesticide sprays have realized that the two accuses of position flow make equipment operation get up more nimble, can be according to the nimble adjustment of orchard load. The design of the protective cover and the return pipe prevents the waste of resources. The fruit setting agent is simple and practical, convenient to use, wide in application range and low in cost, and can greatly reduce labor for pollination and flower and fruit thinning in an orchard.
Drawings
Fig. 1 is a schematic structural diagram of the position-flow double-control sprayer of the invention: (ii) a
FIG. 2 is a rear view of the blind;
FIG. 3 is a side view of the blind;
wherein: 1. a spray device switch; 2. a liquid storage barrel; 3. a solution reflux header pipe; 4. a multi-way joint; 5. solution backflow branch pipe; 6. a nozzle switch; 7. a spray head; 8. a spray rod; 9. a chute; 10. fastening a bolt; 11. a fan blade shaft; 12. louver cover 13, protective cover 14 and fan blades.
FIG. 4 is a photograph showing pollen dispersion in 0.05% Xanthomonas polysaccharide solution.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
As introduced in the background art, most fruit trees are cross pollinated, and if the number of pollinated pollen can be controlled, the problems of flower thinning and fruit thinning of the fruit trees can be fundamentally solved.
Based on the method, the single-variety orchard is built, the position and flow double-control sprayer nozzle is used for liquid pollination, the liquid pollination concentration is adjusted according to the expected yield, and therefore the fruit setting rate of the fruit trees is regulated and controlled in the flowering phase.
The position and flow double-control liquid pollination spraying device comprises a liquid storage barrel (2) and a spray rod (8) connected with the liquid storage barrel, wherein at least one spray head (7) is arranged on the spray rod (8); a protective cover (13) is arranged on the spray head (7); one end of the protective cover (13) is fixed on the spray rod (8), and the other end of the protective cover is provided with an opening; one end of the opening of the protective cover (13) is provided with a shutter cover (12).
Furthermore, a plurality of spray heads (7) are arranged on the spray rod (8), and the interval between the spray heads (7) is 40-50 cm.
Furthermore, a plurality of fan blades (14) are arranged on the louver cover (12); the fan blades (14) are movably connected with a longitudinal fan blade shaft (11).
Furthermore, the lower end of the fan blade shaft (11) is movably connected with a sliding groove (9) fixed on the outer edge of the protective cover (13), and a fastening bolt (10) is arranged on the sliding groove (9).
Furthermore, the bottommost part of one end of the opening of the protective cover (13) is connected with the solution backflow branch pipe (5).
Furthermore, the solution backflow branch pipe (5) is connected with the multi-way joint (4), and one end of the multi-way joint (4) is connected with the liquid storage barrel (2) through the solution backflow header pipe (3).
The double control of the position and the flow is realized by the following method: the length of the spray rod and the number of the spray heads are determined according to the height of a fruit tree to be pollinated and the distribution position of flowers, the angle of fan blades on the louver cover is adjusted according to the distribution position of the flowers and the load of the fruit tree, and the concentration of pollen in the liquid pollination liquid is adjusted according to the fruit setting rate required.
The liquid pollination liquid comprises the following raw materials in parts by weight: 4-10 parts of pollen, 4-6 parts of xanthomonas polysaccharide, 120-180 parts of cane sugar, 10-15 parts of borax and 10000 parts of water. Wherein the xanthomonas polysaccharide can be quickly dissolved in water and has good water solubility. Especially can be dissolved in cold water, can save complicated processing procedures and is convenient to use. However, because of its strong hydrophilicity, if water is directly added and the stirring is not sufficient, the outer layer absorbs water and expands into a micelle, which can prevent water from entering the inner layer, thereby affecting the performance of the effect. Generally, one part of xanthomonas polysaccharide is uniformly mixed with ten parts or more of other dried raw materials, such as sugar, monosodium glutamate, salt and the like in food preparation, and then slowly poured into water in stirring to be soaked for about two hours, and the stirring is continued until the xanthomonas polysaccharide is completely dissolved. This allows for uniform mixing in the solution without sinking to the bottom of the liquid after pollen addition.
In order to make the technical solutions of the present application more clearly understood by those skilled in the art, the technical solutions of the present application will be described in detail below with reference to specific embodiments.
The test materials used in the examples of the present invention are all conventional in the art and commercially available.
Example (b):
preparing liquid pollination liquid: 6g of xanthomonas polysaccharide and 180g of cane sugar are uniformly mixed in advance, 10Kg of hot water at 70 ℃ is slowly added for dissolving, then 10g of borax is added while stirring, the mixture is uniformly kept still for half an hour until the mixture is cooled to room temperature, 4g of pure begonia pollen is added, and the mixture is uniformly mixed and then added into a liquid storage barrel.
Fruit tree pollination:
checking whether air leakage occurs at each joint of the spraying device, and pouring the prepared liquid pollination liquid into the liquid storage barrel (2).
A Fuji apple tree with the height of 3m is selected as a target fruit tree, the number of the spray heads (7) is determined to be 4 according to the height of the target fruit tree and the number of flowers and fruits on the fruit tree, and the spray rod (8) is 2m long. According to the evaluation of the flower quantity of the fruit trees in the garden, the flower fruit setting rate is determined to be 30 percent, and the inflorescence fruit setting rate is determined to be 90 percent.
According to the determined spraying range, the opening and closing angle of the fan blades (14) on the louver cover (12) is adjusted by moving the fan blade shafts (11) up and down, so that the aim of controlling the spraying range and the spraying angle of the liquid pollination liquid is fulfilled. After the angle is adjusted, the position of the fan blade shaft (11) is fixed through the fastening bolt (10), so that the fan blade shaft (11) is fixed on the sliding chute (9), the fan blade shaft is prevented from moving up and down, and the opening and closing angle of the fan blade (14) is prevented from being changed due to the impact of the liquid pollination liquid.
The switch (1) of the spraying device is turned on, the liquid pollination liquid enters the spray rod (8), and the switch (6) of the spray head to be used is turned on, and the liquid pollination liquid is sprayed out from the spray head (7) in a spray form. A part of the liquid pollination liquid sprayed from the spray head (7) is blocked by the fan blade (14) and can not be sprayed. The liquid pollination liquid which is not sprayed out is blocked in the protective cover (13) and flows to the bottommost end in the protective cover (13). The backflow branch pipe (5) connected to the bottommost end of the protective cover (13) is converged to the backflow main pipe (3) through the multi-way connector (4) and flows back to the liquid storage barrel (2), so that the waste of liquid pollination liquid is avoided.
If adjacent orchards exist within 30 meters, an insect-proof net can be arranged in the flowering phase to prevent interference of exogenous pollen;
after the spraying operation is finished, the switch (1) of the spraying device is closed, the residual liquid pollination liquid in the barrel is poured out, the barrel is cleaned by clear water and is inversely dried, and other parts are also cleaned by the clear water and are dried in the shade. When the product is not used any more in a short period, the main components are cleaned, wiped, dried and arranged in a cool and dry place; if the metal parts are not used for a long time, the metal parts are coated with butter to prevent rusting.
Test example 1 liquid pollination solution formulation test
The test was carried out with the male parent being begonia pollen and the female parent being kning wenshang county four-year-old fuji, and 9 100ml erlenmeyer flasks were divided into 3 groups and dissolved in 70 ℃ hot water, respectively. The concentration of the added xanthomonas polysaccharide is 0.04 percent in the first group, the sucrose is added to assist the dissolution, and the proportion (weight ratio) of the xanthomonas polysaccharide to the sucrose is 1: 10,1: 20,1: 30, of a nitrogen-containing gas; the concentration of the added xanthomonas polysaccharide in the second group is 0.05 percent, and the proportion (weight ratio) of the xanthomonas polysaccharide to the sucrose is 1: 10,1: 20,1: 30, of a nitrogen-containing gas; the concentration of the added xanthomonas polysaccharide in the third group is 0.06 percent, and the proportion (weight ratio) of the xanthomonas polysaccharide to the sucrose is 1: 10,1: 20,1: 30, of a nitrogen-containing gas; the time (min) required for complete dissolution was recorded (see table 1).
Table 1: time required for dissolution
Figure BDA0002318796730000051
Experiments show that the ratio of the xanthomonas polysaccharide to the sucrose added into the hot water solution is 1: at 30 f, the time required for the colloid to dissolve uniformly is short. When the solution is cooled to room temperature, 0.04% of pollen is added into 3 colloidal solutions with different concentrations respectively, the solution is stirred uniformly and then is kept stand for half an hour, the uniform dissolution degree of the pollen in the colloidal solution is observed, and the result shows that the pollen is distributed uniformly under the condition that the concentration of the xanthomonas polysaccharide is 0.06%, and the phenomena of pollen floating or sinking and the like do not occur (see table 2 and figure 4).
Table 2:
Figure BDA0002318796730000052
experimental example 2. effect of liquid pollination on fruit setting rate and quality:
three groups of liquid pollination liquids with prepared pollen concentrations of 0.03%, 0.04% and 0.05% are respectively used for pollinating the four-year Fuji fruit trees in Wenshang county, each group is repeated for three times, natural pollination is used as a control, and the specific experimental result is as follows:
(1) effect of liquid pollination on fruit setting rate:
table 3:
fruit setting rate% Repeat one (%) Repeat two (%) Repeat three (%) Average (%)
Contrast (Natural pollination) 36.9 33.5 32.1 34.2
0.03% 27.3 32.0 29.3 29.5
0.04% 50.0 51.0 52.0 51.0
0.05% 63.3 62.4 63.4 63.0
Table 3 shows that when the pollen concentration in the liquid pollination solution is 0.03%, the fruit setting rate is obviously reduced compared with the control; when the pollen concentration in the liquid pollination liquid is 0.04 percent and 0.05 percent, the fruit setting rate is obviously increased compared with the control.
(2) Effect of liquid pollination on fruit weight per fruit:
table 4:
repeat one (g) Repeat two (g) Repeat three (g) Average (g)
Control 276.1 239.2 231.3 248.9
0.03% 246.6 244.8 235.2 242.2
0.04% 257.7 249.8 258.0 255.2
0.05% 251.3 246.8 253.5 250.5
From table 4, liquid pollination at different pollen concentrations had no significant effect on fruit weight alone.
(3) Effect of liquid pollination on fruit firmness:
table 5:
Figure BDA0002318796730000061
from table 5, liquid pollination at different pollen concentrations had no significant effect on fruit firmness.
(4) Effect of liquid pollination on fruit soluble solids:
table 6:
Figure BDA0002318796730000062
from Table 6, liquid pollination at different pollen concentrations can increase soluble solids content of the fruit; but the soluble solid content of the fruits has no obvious difference when the pollen concentration is different.
(5) Effect of liquid pollination on Vc content of fruits:
table 7:
Figure BDA0002318796730000072
from Table 7, it is clear that liquid pollination at different pollen concentrations had no significant effect on the Vc content of the fruits.
(6) Effect of liquid pollination on fruit soluble sugar content:
table 8:
repeat one (%) Repeat two (%) Repeat three (%) Average (%)
Control 8.83 8.51 8.69 8.69
0.03% 10.57 10.16 10.89 10.33
0.04% 10.05 9.95 10.37 10.12
0.05% 9.81` 10.01 10.26 10.37
From Table 8, liquid pollination at different pollen concentrations can increase the soluble sugar content of the fruit; but the soluble sugar content of the fruits has no obvious difference when the pollen concentration is different.
(7) Effect of liquid pollination on titratable acid content of fruit:
table 9:
repeat one (%) Repeat two (%) Repeat three (%) Average (%)
Control 0.29 0.26 0.26 0.27
0.03% 0.22 0.21 0.22 0.22
0.04% 0.24 0.21 0.24 0.23
0.05% 0.24 0.25 0.29 0.26
From Table 9, liquid pollination at different pollen concentrations reduced the titratable acid content of the fruits; as the concentration of pollen increases, the content of titratable acid in the fruit slightly increases.
(8) Effect of liquid pollination on anthocyanin content of pericarp:
table 10:
from Table 10, liquid pollination with different pollen concentrations can increase the anthocyanin content of the pericarp; but the pollen concentration is different, and the content of the anthocyanin in the pericarp of the pollen is not obviously different.
In conclusion, the fruit setting rate of the fruits can be adjusted by controlling the pollen concentration in the pollination solution through liquid pollination, and the fruit setting rate is increased along with the increase of the pollen concentration; the liquid pollination can also improve the soluble sugar content and anthocyanin content of the fruits, reduce the titratable acid content in the fruits, and improve the flavor, color and other qualities of the fruits.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims (10)

1. A position and flow double-control liquid pollination spraying device comprises a liquid storage barrel and a spray rod connected with the liquid storage barrel, and is characterized in that at least one spray head is arranged on the spray rod; the spray head is provided with a protective cover; one end of the protective cover is fixed on the spray rod, and the other end of the protective cover is provided with an opening; and a shutter cover is arranged at one open end of the protective cover.
2. The liquid pollination spray device of claim 1, wherein a plurality of spray heads are arranged on the spray rod, and the spacing between the spray heads is 30-50 cm.
3. The liquid pollination spray device of claim 1, wherein a plurality of fan blades are arranged on the louver cover; the fan blades are movably connected with a longitudinal fan blade shaft.
4. The liquid pollination spray device of claim 3, wherein the lower end of the fan blade shaft is movably connected with a sliding groove fixed on the outer edge of the protective cover, and a fastening bolt is arranged on the sliding groove.
5. The liquid pollination spray device of claim 1, wherein the bottom of the open end of the protective cover is connected with a solution return branch pipe.
6. The liquid pollination spray device of claim 5, wherein the solution return branch pipe is connected with a multi-way joint, and one end of the multi-way joint is connected with the liquid storage barrel through a solution return main pipe.
7. Use of the position flow double control liquid pollination spray device of any one of claims 1-6 in adjusting fruit setting rate of fruit trees.
8. A method for adjusting fruit setting rate of fruit trees is characterized by comprising the following steps:
(1) building a garden for a single variety, or unifying pollinated trees to main cultivated varieties by a high grafting and head changing method, and uniformly shaping and pruning to ensure that the tree height, the crown diameter and the branch structure are consistent as much as possible;
(2) determining the length of a spray rod and the number of spray heads according to the height of a fruit tree to be pollinated and the distribution position of flowers, adjusting the angle of fan blades on a louver cover according to the distribution position of the flowers and the load of the fruit tree, and adjusting the concentration of pollen in liquid pollination liquid according to the fruit setting rate required to be achieved; the position flow double-control liquid pollination spraying device of any one of claims 1-6 is used for spraying liquid pollination liquid on fruit trees to be pollinated.
9. The method of claim 8, wherein the liquid pollination fluid comprises the following raw materials in parts by weight: 4-10 parts of pollen, 4-6 parts of xanthomonas polysaccharide, 120-180 parts of cane sugar, 10-15 parts of borax and 10000 parts of water.
10. The method of claim 8 or 9, wherein the liquid pollination liquid is prepared by:
A. adding weighed xanthomonas polysaccharide and sucrose into hot water at 70-80 ℃, continuously stirring until the xanthomonas polysaccharide and the sucrose are completely dissolved, adding borax and stirring;
B. and D, when the solution is cooled to room temperature, adding the pollen into the solution prepared in the step A, and continuously stirring until the pollen is uniformly mixed, thus completing the preparation.
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CN208676032U (en) * 2018-08-15 2019-04-02 中国林业科学研究院亚热带林业研究所 A kind of artificial pollination pollination device
CN208924898U (en) * 2018-10-15 2019-06-04 李帅 Hazel Liquid pollen pollination device
CN110100725A (en) * 2019-06-14 2019-08-09 南京农业大学 A kind of apple liquid pollination medicament and pollinating method

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Inventor after: Shen Xiang

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Correct: Shen Xiang|Yang Lu|Wang Yunyun|Zhang Lulu|Mao Yunfei|Yin Xinyu|Yin Yijun|Su Xiafei|Liu Yeping|Pang Huiling|Hu Yanli|Mao Zhiquan|Chen Xuesen|Wang Wenli|Wei Shaochong|Chen Xiaoliu

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