CN110709512A - 一种锰过氧化物酶及其基因和在霉菌毒素脱毒上的应用 - Google Patents

一种锰过氧化物酶及其基因和在霉菌毒素脱毒上的应用 Download PDF

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CN110709512A
CN110709512A CN201780091407.1A CN201780091407A CN110709512A CN 110709512 A CN110709512 A CN 110709512A CN 201780091407 A CN201780091407 A CN 201780091407A CN 110709512 A CN110709512 A CN 110709512A
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seq
mnp
manganese peroxidase
amino acid
acid sequence
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CN201780091407.1A
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姚斌
苏小运
秦星
罗会颖
黄火清
柏映国
王苑
王亚茹
马锐
涂涛
马建爽
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Institute of Animal Science of CAAS
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Feed Research Institute of Chinese Academy of Agricultural Sciences
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01—Peroxidases (1.11.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01—Peroxidases (1.11.1)
    • C12Y111/01013—Manganese peroxidase (1.11.1.13)

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  • Enzymes And Modification Thereof (AREA)
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Abstract

锰过氧化物酶在霉菌毒素脱毒上的应用，具体地，本发明提供了五种锰过氧化物酶(MnP‑1、MnP‑2、MnP‑4、MnP‑5和MnP‑6)及其基因和应用。本发明提供了五种来源于木质纤维素降解菌的锰过氧化物酶(MnP‑1、MnP‑2、MnP‑4、MnP‑5和MnP‑6)，其氨基酸序列如SEQ ID NO.1、SEQ ID NO.4、SEQ ID NO.7、SEQ ID NO.10和SEQ ID NO.13所示。

Description

PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN201780091407.1A 2017-05-31 2017-05-31 一种锰过氧化物酶及其基因和在霉菌毒素脱毒上的应用 Pending CN110709512A (zh)

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PCT/CN2017/086534 WO2018218476A1 (zh) 2017-05-31 2017-05-31 一种锰过氧化物酶及其基因和在霉菌毒素脱毒上的应用

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US (1) US11634694B2 (zh)
EP (1) EP3674401A4 (zh)
JP (1) JP7072745B2 (zh)
CN (1) CN110709512A (zh)
WO (1) WO2018218476A1 (zh)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112195163A (zh) * 2020-11-11 2021-01-08 安徽大学 一种不依赖锰离子的锰过氧化物酶突变体及其应用
CN112961791A (zh) * 2021-02-24 2021-06-15 华南理工大学 一种非特异性过氧合酶的重组菌株及其构建方法和应用
CN113604447A (zh) * 2021-09-28 2021-11-05 中国农业科学院北京畜牧兽医研究所 锰过氧化物酶用于降解桔霉素的应用及方法
CN113693189A (zh) * 2021-09-28 2021-11-26 中国农业科学院北京畜牧兽医研究所 锰过氧化物酶用于降解棒曲霉素的应用及方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109370995B (zh) * 2018-12-11 2021-05-25 中国农业科学院北京畜牧兽医研究所 锰过氧化物酶CsMnP及其编码基因和应用
CN111154737B (zh) * 2020-01-14 2022-02-01 江南大学 一种能降解黄曲霉毒素b1的锰过氧化物酶及其应用
CN113528477B (zh) * 2021-08-20 2023-08-08 江南大学 一种能降解黄曲霉毒素b1的锰过氧化物酶的突变体构建方法及其应用

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CN101066095A (zh) * 2007-06-01 2007-11-07 上海三维饲料添加剂有限公司 一种饲料霉菌毒素脱毒添加剂及其使用方法
CN104232555A (zh) * 2014-09-12 2014-12-24 上海交通大学 基于锰过氧化物酶的工程菌及其实现方法
CN106148294A (zh) * 2016-07-26 2016-11-23 中国农业科学院饲料研究所 一种锰过氧化物酶mnp‑1及其基因和应用
CN106434584A (zh) * 2016-07-26 2017-02-22 中国农业科学院饲料研究所 一种锰过氧化物酶mnp‑2及其基因和应用
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CN104232555A (zh) * 2014-09-12 2014-12-24 上海交通大学 基于锰过氧化物酶的工程菌及其实现方法
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112195163A (zh) * 2020-11-11 2021-01-08 安徽大学 一种不依赖锰离子的锰过氧化物酶突变体及其应用
CN112195163B (zh) * 2020-11-11 2022-06-28 安徽大学 一种不依赖锰离子的锰过氧化物酶突变体及其应用
CN112961791A (zh) * 2021-02-24 2021-06-15 华南理工大学 一种非特异性过氧合酶的重组菌株及其构建方法和应用
CN113604447A (zh) * 2021-09-28 2021-11-05 中国农业科学院北京畜牧兽医研究所 锰过氧化物酶用于降解桔霉素的应用及方法
CN113693189A (zh) * 2021-09-28 2021-11-26 中国农业科学院北京畜牧兽医研究所 锰过氧化物酶用于降解棒曲霉素的应用及方法
CN113604447B (zh) * 2021-09-28 2021-12-24 中国农业科学院北京畜牧兽医研究所 锰过氧化物酶用于降解桔霉素的应用及方法
CN113693189B (zh) * 2021-09-28 2023-11-17 中国农业科学院北京畜牧兽医研究所 锰过氧化物酶用于降解棒曲霉素的应用及方法

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JP2020529829A (ja) 2020-10-15
EP3674401A4 (en) 2021-06-23
US20210139864A1 (en) 2021-05-13
WO2018218476A1 (zh) 2018-12-06
EP3674401A1 (en) 2020-07-01
JP7072745B2 (ja) 2022-05-23
US11634694B2 (en) 2023-04-25

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